Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0007310

Physical Location & Seq

Genomic Coordinates Reverse (-)

31433498 .. 31440833

Gene Structure 7336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0179100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGCTTCTTCTTCTTCTTCTTCAACTCCAGCGACGACCACGACGACGACCGCGACGACGTCGTCTTCCGCCATGAAAAGAGTCGCGCTCACTCTCACAACGTCGTGCCGGAGCTTTCCTCTTCTCTTCCTCCTCCTGATTTCCACCAACATCACCGCCGGCGTCGCTCTCGACAACGCCGTCTTGAGCGTCAAGTACAAATACGCCGGCCGCGACCGCACTCTCAGCGCCCTAAAAGCTCACGACACCAAACGCACTCTCCGATTTCTCGCCGGCGTCGATCTCCCTCTCGGCGGCTCCGGCCGCCCTGACGCCGTCGGATTATACTATGCGAAAATTGGAATAGGAACTCCTCCTAGAGACTACTATGTACAAGTTGATACTGGAAGTGATATTATGTGGGTCAACTGTATTCAATGCAAGGATTGCCCTGAAAGAAGCTCACTTGGTTTTGAGCTATCACTATATGATATCAAGCAGTCTTCCACTGGTAAACTGATTCCTTGTGATCAAGAGTTTTGCGTGGCGGTGAATAATGGTCCATTGGCTGGATGTACGGCCAATATGTCGTGTCCTTATTTACAAATTTACGGAGACGGCACTTCCACTGCTGGTTACTTTGTCAAAGATATTGTGTTGTATGACCAAGTGTCGGGTGACCTCAAGACTAGCTCTTCCAATGGAAGCGTCGTTTTTGGGTGTGGTGCTACACAATCTGGTGATATAGGTTCATCTGGTGAAGAAGCACTTGATGGAATTCTTGGCTTTGGAAAGTCCAATTCATCCATAATTTCACAGCTAGGTTCATCAGGAAAAGTGAAGAAGATGTTTGCTCACTGCTTAGATGGAAAGAATGGAGGCGGTATCTTTGCTATTGGGCGTGTTGTGCAGCCGAAAGTTAACATGACTCCATTGGTACCAAACCAGCCGCACTACAATGTCAATATGAAGGCAGTTCAAGTCGGCCATGCTTTCCTAAATATTTCTACAGATGTATTTGAAGCGGGAGATAGAAAAGGGACTATAATTGACAGCGGTACAACTTTGGCCTATCTTCCAGACGTGGTGTACGAGCCCTTAGTAAGTAAGATCTTCTCTCAGCAGCCTGATTTGAAAATTCACACTCTTCGTGGCGAGTATACATGCTTTGAGTACTCGGGAAGTGTTGATGATGGATTTCCTGCAGTCACCTTTTATTTTGAAAATTCACTTTCTTTGAAGGTTTATCCACATGACTACTTGTTCCCCTCTGAAGGCTATTGGTGCATTGGTTGGCAAAACAGTGGGTTGCAATCCAGGGATACTAAGAACTTGACCCTTTTGGGAGATTTGGTACTTTCGAATAAGCTAGTGTTGTATGATCTTGAAAATCAAGTCATTGGATGGACTGAGTATAACTGCTCATCAAGTATCAAGGTACAGGATGAACAGAGTGGAACAGTGCATTTAGTAGGTTCCCACTACATCTCTTCTTCTGCTTGCCATTTGAATCACCAATGGACTATAAACTTCTTCCTTACGCTGCTGCTGTACAGTTTACTTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 zf-FPG_IleRS

Protein Analysis

515

Amino Acids

55.64

Weight (kDa)

5.93

Isoelectric Point (pI)

37.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TAXi_N	PF14543	109 - 293	3e-37	Xylanase inhibitor N-terminal
Asp	PF00026	109 - 463	7.5e-21	Eukaryotic aspartyl protease
TAXi_C	PF14541	311 - 463	1.7e-17	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49030 AT5G49030 AT5G49030
fragaria_vesca	FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060
malus_domestica	MD13G1168400.v1.1 MD16G1168800.v1.1
prunus_persica	Prupe.1G136500_v2.0.a1 Prupe.1G136500_v2.0.a1
pyrus_communis	pycom13g14400 pycom16g14210
rosa_chinensis	RchiOBHm_Chr4g0422271
rosa_laevigata	RLG00000007623
rosa_multiflora	Rmu_sc0013245.1_g000018
rosa_roxburghii	Rroxscaffold_5G00364380
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238300 Rh4BG241400 Rh4CG253300 Rh4DG237000
rosa_wichuraiana	Rw4G020620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	61, 179
AatII	GACGTC	1 cut(s)	62
Acc65I	GGTACC	1 cut(s)	916
AccB1I	GGYRCC	1 cut(s)	916
AccB7I	CCANNNNNTGG	1 cut(s)	548
AccI	GTMKAC	1 cut(s)	1139
AccII	CGCG	3 cut(s)	53, 86, 213
AcoI	YGGCCR	4 cut(s)	208, 301, 558, 964
AcsI	RAATTY	4 cut(s)	585, 756, 1116, 1204
AcuI	CTGAAG	1 cut(s)	1272
AcyI	GRCGYC	4 cut(s)	59, 162, 276, 312
AfiI	CCNNNNNNNGG	4 cut(s)	293, 548, 652, 1253
AgsI	TTSAA	9 cut(s)	24, 416, 959, 1001, 1114, 1202, 1219, 1367, 1489
AhdI	GACNNNNNGTC	1 cut(s)	649
AjiI	CACGTC	1 cut(s)	1063
AjnI	CCWGG	1 cut(s)	1295
AluBI	AGCT	7 cut(s)	114, 239, 441, 457, 672, 799, 1348
AluI	AGCT	7 cut(s)	114, 239, 441, 457, 672, 799, 1348
Alw26I	GTCTC	2 cut(s)	354, 588
Ama87I	CYCGRG	1 cut(s)	1156
AoxI	GGCC	5 cut(s)	208, 301, 558, 964, 1047
ApeKI	GCWGC	4 cut(s)	889, 1102, 1522, 1525
ApoI	RAATTY	4 cut(s)	585, 756, 1116, 1204
Asp718I	GGTACC	1 cut(s)	916
AspLEI	GCGC	2 cut(s)	88, 230
AspS9I	GGNCC	1 cut(s)	539
AsuHPI	GGTGA	7 cut(s)	145, 541, 668, 731, 749, 1180, 1484
AsuII	TTCGAA	1 cut(s)	1340
AvaI	CYCGRG	1 cut(s)	1156
AvaII	GGWCC	1 cut(s)	539
BanI	GGYRCC	1 cut(s)	916
BanII	GRGCYC	1 cut(s)	1077
BbsI	GAAGAC	2 cut(s)	57, 474
BbvI	GCAGC	4 cut(s)	901, 1114, 1509, 1512
BccI	CCATC	4 cut(s)	746, 839, 1166, 1377
BceAI	ACGGC	4 cut(s)	164, 299, 573, 613
BciT130I	CCWGG	1 cut(s)	1297
BciVI	GTATCC	1 cut(s)	1294
BclI	TGATCA	1 cut(s)	508
BcoDI	GTCTC	2 cut(s)	354, 588
BfaI	CTAG	4 cut(s)	357, 669, 800, 1349
BfmI	CTRYAG	2 cut(s)	987, 1182
BfoI	RGCGCY	1 cut(s)	231
BfuI	GTATCC	1 cut(s)	1294
BglII	AGATCT	1 cut(s)	1089
BisI	GCNGC	8 cut(s)	211, 295, 304, 890, 929, 1103, 1523, 1526
BlsI	GCNGC	8 cut(s)	212, 296, 305, 891, 930, 1104, 1524, 1527
BmcAI	AGTACT	1 cut(s)	1154
Bme1390I	CCNGG	1 cut(s)	1297
Bme18I	GGWCC	1 cut(s)	539
BmeRI	GACNNNNNGTC	1 cut(s)	649
BmeT110I	CYCGRG	1 cut(s)	1156
BmgBI	CACGTC	1 cut(s)	1063
BmgT120I	GGNCC	1 cut(s)	539
BmiI	GGNNCC	3 cut(s)	298, 918, 1456
BmrFI	CCNGG	1 cut(s)	1297
BpiI	GAAGAC	2 cut(s)	57, 474
BplI	GAGNNNNNCTC	4 cut(s)	72, 104, 103, 135
BpmI	CTGGAG	1 cut(s)	12
Bpu14I	TTCGAA	1 cut(s)	1340
BpuEI	CTTGAG	2 cut(s)	205, 647
BsaHI	GRCGYC	4 cut(s)	59, 162, 276, 312
BsaJI	CCNNGG	1 cut(s)	1296
BsaXI	ACNNNNNCTCC	4 cut(s)	243, 273, 1317, 1347
Bsc4I	CCNNNNNNNGG	4 cut(s)	293, 548, 652, 1253
Bse118I	RCCGGY	3 cut(s)	158, 206, 272
Bse1I	ACTGG	2 cut(s)	388, 493
BseBI	CCWGG	1 cut(s)	1297
BseDI	CCNNGG	1 cut(s)	1296
BseGI	GGATG	4 cut(s)	557, 782, 1388, 1429
BseLI	CCNNNNNNNGG	4 cut(s)	293, 548, 652, 1253
BseMII	CTCAG	3 cut(s)	238, 1112, 1380
BseNI	ACTGG	2 cut(s)	388, 493
BseRI	GAGGAG	2 cut(s)	122, 342
BseX3I	CGGCCG	2 cut(s)	208, 301
BseXI	GCAGC	4 cut(s)	901, 1114, 1509, 1512
BsgI	GTGCAG	1 cut(s)	908
Bsh1236I	CGCG	3 cut(s)	53, 86, 213
Bsh1285I	CGRYCG	4 cut(s)	51, 211, 217, 304
BshFI	GGCC	5 cut(s)	210, 303, 560, 966, 1049
BshNI	GGYRCC	1 cut(s)	916
BsiEI	CGRYCG	4 cut(s)	51, 211, 217, 304
BsiHKCI	CYCGRG	1 cut(s)	1156
BsiSI	CCGG	5 cut(s)	109, 159, 207, 273, 300
BslFI	GGGAC	1 cut(s)	1033
BslI	CCNNNNNNNGG	4 cut(s)	293, 548, 652, 1253
BsmAI	GTCTC	2 cut(s)	354, 588
BsmBI	CGTCTC	1 cut(s)	588
BsmFI	GGGAC	1 cut(s)	1033
BsnI	GGCC	5 cut(s)	210, 303, 560, 966, 1049
BsoBI	CYCGRG	1 cut(s)	1156
Bsp119I	TTCGAA	1 cut(s)	1340
Bsp1286I	GDGCHC	1 cut(s)	1077
Bsp1407I	TGTACA	2 cut(s)	370, 1530
Bsp143I	GATC	4 cut(s)	280, 508, 1089, 1360
BspANI	GGCC	5 cut(s)	210, 303, 560, 966, 1049
BspCNI	CTCAG	3 cut(s)	237, 1111, 1381
BspFNI	CGCG	3 cut(s)	53, 86, 213
BspLI	GGNNCC	3 cut(s)	298, 918, 1456
BspMAI	CTGCAG	1 cut(s)	1186
BspQI	GCTCTTC	1 cut(s)	679
BspT104I	TTCGAA	1 cut(s)	1340
BspT107I	GGYRCC	1 cut(s)	916
BsrFI	RCCGGY	3 cut(s)	158, 206, 272
BsrGI	TGTACA	2 cut(s)	370, 1530
BsrI	ACTGG	2 cut(s)	388, 493
BssAI	RCCGGY	3 cut(s)	158, 206, 272
BssECI	CCNNGG	1 cut(s)	1296
BssMI	GATC	4 cut(s)	280, 508, 1089, 1360
BssNAI	GTATAC	1 cut(s)	1140
BssNI	GRCGYC	4 cut(s)	59, 162, 276, 312
Bst1107I	GTATAC	1 cut(s)	1140
Bst2UI	CCWGG	1 cut(s)	1297
Bst4CI	ACNGT	4 cut(s)	410, 1283, 1441, 1535
Bst6I	CTCTTC	5 cut(s)	126, 131, 679, 1131, 1474
BstACI	GRCGYC	4 cut(s)	59, 162, 276, 312
BstAPI	GCANNNNNTGC	1 cut(s)	426
BstAUI	TGTACA	2 cut(s)	370, 1530
BstBI	TTCGAA	1 cut(s)	1340
BstC8I	GCNNGC	4 cut(s)	160, 208, 274, 1480
BstDEI	CTNAG	6 cut(s)	224, 841, 1078, 1098, 1305, 1389
BstEII	GGTNACC	1 cut(s)	656
BstF5I	GGATG	4 cut(s)	557, 782, 1388, 1429
BstFNI	CGCG	3 cut(s)	53, 86, 213
BstH2I	RGCGCY	1 cut(s)	231
BstHHI	GCGC	2 cut(s)	88, 230
BstKTI	GATC	4 cut(s)	283, 511, 1092, 1363
BstMAI	GTCTC	2 cut(s)	354, 588
BstMBI	GATC	4 cut(s)	280, 508, 1089, 1360
BstMCI	CGRYCG	4 cut(s)	51, 211, 217, 304
BstMWI	GCNNNNNNNGC	9 cut(s)	164, 216, 225, 236, 300, 303, 426, 886, 1263
BstNI	CCWGG	1 cut(s)	1297
BstNSI	RCATGY	1 cut(s)	1146
BstPI	GGTNACC	1 cut(s)	656
BstSCI	CCNGG	1 cut(s)	1295
BstSFI	CTRYAG	2 cut(s)	987, 1182
BstUI	CGCG	3 cut(s)	53, 86, 213
BstV1I	GCAGC	4 cut(s)	901, 1114, 1509, 1512
BstV2I	GAAGAC	2 cut(s)	57, 474
BstX2I	RGATCY	1 cut(s)	1089
BstYI	RGATCY	1 cut(s)	1089
BstZ17I	GTATAC	1 cut(s)	1140
BstZI	CGGCCG	2 cut(s)	208, 301
BsuI	GTATCC	1 cut(s)	1294
BsuRI	GGCC	5 cut(s)	210, 303, 560, 966, 1049
BtrI	CACGTC	1 cut(s)	1063
BtsCI	GGATG	4 cut(s)	557, 782, 1388, 1429
BtsI	GCAGTG	2 cut(s)	606, 835
BtsIMutI	CAGTG	5 cut(s)	486, 606, 835, 1288, 1446
Cac8I	GCNNGC	4 cut(s)	160, 208, 274, 1480
CfoI	GCGC	2 cut(s)	88, 230
Cfr10I	RCCGGY	3 cut(s)	158, 206, 272
Cfr13I	GGNCC	1 cut(s)	539
CseI	GACGC	5 cut(s)	151, 178, 265, 320, 676
CviAII	CATG	5 cut(s)	73, 904, 968, 1143, 1232
DdeI	CTNAG	6 cut(s)	224, 841, 1078, 1098, 1305, 1389
DpnI	GATC	4 cut(s)	282, 510, 1091, 1362
DpnII	GATC	4 cut(s)	280, 508, 1089, 1360
DrdI	GACNNNNNNGTC	2 cut(s)	61, 179
DriI	GACNNNNNGTC	1 cut(s)	649
DseDI	GACNNNNNNGTC	2 cut(s)	61, 179
EaeI	YGGCCR	4 cut(s)	208, 301, 558, 964
EagI	CGGCCG	2 cut(s)	208, 301
Eam1104I	CTCTTC	5 cut(s)	126, 131, 679, 1131, 1474
Eam1105I	GACNNNNNGTC	1 cut(s)	649
EarI	CTCTTC	5 cut(s)	126, 131, 679, 1131, 1474
EciI	GGCGGA	1 cut(s)	58
EclXI	CGGCCG	2 cut(s)	208, 301
Eco24I	GRGCYC	1 cut(s)	1077
Eco32I	GATATC	1 cut(s)	472
Eco47I	GGWCC	1 cut(s)	539
Eco52I	CGGCCG	2 cut(s)	208, 301
Eco57I	CTGAAG	1 cut(s)	1272
Eco88I	CYCGRG	1 cut(s)	1156
Eco91I	GGTNACC	1 cut(s)	656
EcoO65I	GGTNACC	1 cut(s)	656
EcoRI	GAATTC	1 cut(s)	756
EcoRII	CCWGG	1 cut(s)	1295
EcoRV	GATATC	1 cut(s)	472
EcoT38I	GRGCYC	1 cut(s)	1077
Esp3I	CGTCTC	1 cut(s)	588
FaeI	CATG	5 cut(s)	76, 907, 971, 1146, 1235
FalI	AAGNNNNNCTT	2 cut(s)	732, 764
FaqI	GGGAC	1 cut(s)	1033
FatI	CATG	5 cut(s)	72, 903, 967, 1142, 1231
FauI	CCCGC	1 cut(s)	997
FbaI	TGATCA	1 cut(s)	508
FblI	GTMKAC	1 cut(s)	1139
Fnu4HI	GCNGC	8 cut(s)	211, 295, 304, 890, 929, 1103, 1523, 1526
FokI	GGATG	4 cut(s)	564, 769, 1395, 1436
FriOI	GRGCYC	1 cut(s)	1077
Fsp4HI	GCNGC	8 cut(s)	211, 295, 304, 890, 929, 1103, 1523, 1526
FspBI	CTAG	4 cut(s)	357, 669, 800, 1349
GlaI	GCGC	2 cut(s)	87, 229
GluI	GCNGC	8 cut(s)	211, 295, 304, 890, 929, 1103, 1523, 1526
GsuI	CTGGAG	1 cut(s)	12
HaeII	RGCGCY	1 cut(s)	231
HaeIII	GGCC	5 cut(s)	210, 303, 560, 966, 1049
HapII	CCGG	5 cut(s)	109, 159, 207, 273, 300
HgaI	GACGC	5 cut(s)	151, 178, 265, 320, 676
HhaI	GCGC	2 cut(s)	88, 230
Hin1I	GRCGYC	4 cut(s)	59, 162, 276, 312
Hin1II	CATG	5 cut(s)	76, 907, 971, 1146, 1235
Hin6I	GCGC	2 cut(s)	86, 228
HinP1I	GCGC	2 cut(s)	86, 228
HincII	GTYRAC	2 cut(s)	406, 901
HindII	GTYRAC	2 cut(s)	406, 901
HinfI	GANTC	4 cut(s)	81, 499, 907, 1489
HpaI	GTTAAC	1 cut(s)	901
HpaII	CCGG	5 cut(s)	109, 159, 207, 273, 300
HphI	GGTGA	7 cut(s)	145, 541, 668, 731, 749, 1180, 1484
Hpy166II	GTNNAC	6 cut(s)	406, 494, 901, 1069, 1140, 1538
Hpy188I	TCNGA	3 cut(s)	263, 320, 1252
Hpy8I	GTNNAC	6 cut(s)	406, 494, 901, 1069, 1140, 1538
HpyAV	CCTTC	3 cut(s)	943, 1213, 1247
HpyCH4III	ACNGT	4 cut(s)	410, 1283, 1441, 1535
HpyCH4IV	ACGT	3 cut(s)	59, 101, 1062
HpyCH4V	TGCA	6 cut(s)	420, 889, 1184, 1266, 1291, 1444
HpyF10VI	GCNNNNNNNGC	9 cut(s)	164, 216, 225, 236, 300, 303, 426, 886, 1263
HpyF3I	CTNAG	6 cut(s)	224, 841, 1078, 1098, 1305, 1389
HpySE526I	ACGT	3 cut(s)	59, 101, 1062
Hsp92I	GRCGYC	4 cut(s)	59, 162, 276, 312
Hsp92II	CATG	5 cut(s)	76, 907, 971, 1146, 1235
HspAI	GCGC	2 cut(s)	86, 228
KpnI	GGTACC	1 cut(s)	920
KroI	GCCGGC	3 cut(s)	158, 206, 272
KroNI	GCCGGC	3 cut(s)	160, 208, 274
Ksp22I	TGATCA	1 cut(s)	508
KspAI	GTTAAC	1 cut(s)	901
Kzo9I	GATC	4 cut(s)	280, 508, 1089, 1360
LguI	GCTCTTC	1 cut(s)	679
LmnI	GCTCC	2 cut(s)	111, 302
Lsp1109I	GCAGC	4 cut(s)	901, 1114, 1509, 1512
MaeI	CTAG	4 cut(s)	357, 669, 800, 1349
MaeII	ACGT	3 cut(s)	59, 101, 1062
MaeIII	GTNAC	3 cut(s)	614, 656, 1186
MalI	GATC	4 cut(s)	282, 510, 1091, 1362
MboI	GATC	4 cut(s)	280, 508, 1089, 1360
MflI	RGATCY	1 cut(s)	1089
MhlI	GDGCHC	1 cut(s)	1077
MluCI	AATT	8 cut(s)	336, 585, 756, 778, 789, 1026, 1116, 1204
MlyI	GAGTC	2 cut(s)	90, 901
MmeI	TCCRAC	1 cut(s)	298
MnlI	CCTC	8 cut(s)	129, 140, 143, 297, 363, 671, 851, 1258
MreI	CGCCGGCG	2 cut(s)	158, 272
MroNI	GCCGGC	3 cut(s)	158, 206, 272
MseI	TTAA	1 cut(s)	900
MslI	CAYNNNNRTG	1 cut(s)	936
MspA1I	CMGCKG	1 cut(s)	1035
MspI	CCGG	5 cut(s)	109, 159, 207, 273, 300
MspR9I	CCNGG	1 cut(s)	1297
MvaI	CCWGG	1 cut(s)	1297
MvnI	CGCG	3 cut(s)	53, 86, 213
MwoI	GCNNNNNNNGC	9 cut(s)	164, 216, 225, 236, 300, 303, 426, 886, 1263
NaeI	GCCGGC	3 cut(s)	160, 208, 274
NdeII	GATC	4 cut(s)	280, 508, 1089, 1360
NgoMIV	GCCGGC	3 cut(s)	158, 206, 272
NlaIII	CATG	5 cut(s)	76, 907, 971, 1146, 1235
NlaIV	GGNNCC	3 cut(s)	298, 918, 1456
NmeAIII	GCCGAG	1 cut(s)	270
NmuCI	GTSAC	2 cut(s)	656, 1186
NspI	RCATGY	1 cut(s)	1146
NspV	TTCGAA	1 cut(s)	1340
PciSI	GCTCTTC	1 cut(s)	679
PcsI	WCGNNNNNNNCGW	8 cut(s)	41, 50, 53, 177, 210, 259, 276, 1068
PdiI	GCCGGC	3 cut(s)	160, 208, 274
PfeI	GAWTC	2 cut(s)	499, 1489
PflFI	GACNNNGTC	3 cut(s)	58, 61, 314
PflMI	CCANNNNNTGG	1 cut(s)	548
PkrI	GCNGC	8 cut(s)	212, 296, 305, 891, 930, 1104, 1524, 1527
PleI	GAGTC	2 cut(s)	89, 901
PpsI	GAGTC	2 cut(s)	89, 901
Psp6I	CCWGG	1 cut(s)	1295
PspEI	GGTNACC	1 cut(s)	656
PspGI	CCWGG	1 cut(s)	1295
PspN4I	GGNNCC	3 cut(s)	298, 918, 1456
PspPI	GGNCC	1 cut(s)	539
PstI	CTGCAG	1 cut(s)	1186
PsuI	RGATCY	1 cut(s)	1089
PsyI	GACNNNGTC	3 cut(s)	58, 61, 314
RseI	CAYNNNNRTG	1 cut(s)	936
SapI	GCTCTTC	1 cut(s)	679
SaqAI	TTAA	1 cut(s)	900
SatI	GCNGC	8 cut(s)	211, 295, 304, 890, 929, 1103, 1523, 1526
Sau3AI	GATC	4 cut(s)	280, 508, 1089, 1360
Sau96I	GGNCC	1 cut(s)	539
ScaI	AGTACT	1 cut(s)	1154
SchI	GAGTC	2 cut(s)	90, 901
ScrFI	CCNGG	1 cut(s)	1297
SduI	GDGCHC	1 cut(s)	1077
SfcI	CTRYAG	2 cut(s)	987, 1182
SfuI	TTCGAA	1 cut(s)	1340
SgrAI	CRCCGGYG	2 cut(s)	158, 272
SinI	GGWCC	1 cut(s)	539
SmiMI	CAYNNNNRTG	1 cut(s)	936
SmlI	CTYRAG	2 cut(s)	184, 662
SmoI	CTYRAG	2 cut(s)	184, 662
Sse9I	AATT	8 cut(s)	336, 585, 756, 778, 789, 1026, 1116, 1204
SspI	AATATT	1 cut(s)	982
SspMI	CTAG	4 cut(s)	357, 669, 800, 1349
StyD4I	CCNGG	1 cut(s)	1295
TaaI	ACNGT	4 cut(s)	410, 1283, 1441, 1535
TaiI	ACGT	3 cut(s)	62, 104, 1065
TaqI	TCGA	3 cut(s)	171, 279, 1340
TasI	AATT	8 cut(s)	336, 585, 756, 778, 789, 1026, 1116, 1204
TatI	WGTACW	4 cut(s)	195, 370, 1152, 1530
TauI	GCSGC	4 cut(s)	213, 297, 306, 931
TfiI	GAWTC	2 cut(s)	499, 1489
Tru1I	TTAA	1 cut(s)	900
Tru9I	TTAA	1 cut(s)	900
TscAI	CASTG	5 cut(s)	493, 613, 842, 1288, 1446
TseFI	GTSAC	2 cut(s)	656, 1186
TseI	GCWGC	4 cut(s)	889, 1102, 1522, 1525
Tsp45I	GTSAC	2 cut(s)	656, 1186
TspDTI	ATGAA	6 cut(s)	89, 720, 771, 795, 962, 1440
TspGWI	ACGGA	1 cut(s)	606
TspRI	CASTG	5 cut(s)	493, 613, 842, 1288, 1446
Tth111I	GACNNNGTC	3 cut(s)	58, 61, 314
Van91I	CCANNNNNTGG	1 cut(s)	548
VpaK11BI	GGWCC	1 cut(s)	539
XapI	RAATTY	4 cut(s)	585, 756, 1116, 1204
XceI	RCATGY	1 cut(s)	1146
XmiI	GTMKAC	1 cut(s)	1139
XspI	CTAG	4 cut(s)	357, 669, 800, 1349
ZraI	GACGTC	1 cut(s)	60
ZrmI	AGTACT	1 cut(s)	1154

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.