Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0007310

Physical Location & Seq

Genomic Coordinates Forward (+)

44461011 .. 44474405

Gene Structure 13395 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG237000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGAGAGCGAATTCTTCAACACGAGAGCCTGAACTTCAGAAAATATGGGAGGACAATCAAGTGTTCAAGAGAGTAGTTAGTAAAAATACAGGGGAAAACTTCATTCTTCATGATGGTCCTCCGTACGCCAATGGTGATCTGCACATCGGCCATGCTCTAAATAAGATTTTGAAGGATATGATTAATCGTTATAAGCTTCTTCAAAATTATAAAGTTCATTATGTGCCTGGTTGGGATTGTCATGGCTTACCAATTGAATTGAAAGTCCTGCAATCCCTTGATCAAGCTGCTCGAAAGGATCTCACACCAATAAAGTTGAGAGCGAAGGCAGCTAAGTTTGCCAAGCAAACAGTTAAAAATCAGATGGAATCATTTAAGCGTTATGGAATATGGGCAGACTGGAATAATCCCTACCTGACTCTTGATCCAGAATATGAAGCTGCTCAGATAGAAGTGTTTGGTCAGATGGTCCTTCAAGGTTTTATCTACAGAGGCAGGAAACCAGTTCACTGGAGTCCCTCATCACGAACTGCCCTTGCAGAGGCTGAGTTGGAGTATCCGGAGAGTCATGTTTCAAGGAGCATCTATACCGCTTTCAAATTAGTGAGTGCACCTCCAACTTTAGGTGGCTTATTGAATGAGTATCCAAATGTTTGCTTGGCCATTTGGACAACGACTCCCTGGACTATTCCAGCAAATGCCGCGGTTGCAGTGAATGCCAAGCTTATATATGCCATTGTTGAAGTACAGTCAGTCTCCGAAGATGTTTCTTCACCTGCCGGAAATAGCAAGCAAACGCCTGCTAATTTTCTCAAGGAAGAAAAGAAGCCTTTCCTCATTGTTGCTTCAGATCTTGTGCCAACTCTAGAAGCAAAATGGGGCCTGAAGCTTGTTGTTAGGAAAAGGGTGTCAGGTTCAGATCTTGAGAACTGCAGGTATATCCATCCAATATTTAATAGGGAATGCTCAGTTGTGATTGGAGGAGATTATATCACAACAGAGTCGGGAACTGGACTGGTCCATACAGCTCCTGGACATGGTCAGGAAGATTATGTCACTGGCCTTAAGTATGGACTGCCTATTCTTTCTCCAGTAGATGATGATGGAAAGTTCACAGAAGAAGCTGGAAAATTTTGTGGGCTTGATGTACTTGCTGATGGTAATATTGCTGTTGTGAAATACTTGGATGAGCATTTGTCACTGATCATGGAAGAATCCTACAAACATAAGTATCCATATGATTGGAGAACAAAAAAACCCACTATATTTAGGGCAACTGAGCAATGGTTTGCATCAGTGGAAGGCTTTCGTGAGGCTGTTATGGATGCAATTGGCAATGTAAAATGGATTCCAGCTAAGGCAGAAAACAGAATATCTGCAATGACTTCTAGCCGCTCTGATTGGTGTATATCGCGGCAGAGGACTTGGGGTGTTCCAATTCCAGTCTTTTATCACCTCCAGTCAAAGGAACCTCTAATGAATGAGGAGACTATTGATCATATCAAGTCTATAATTTCTGAAAAGGGTAGTGATGCATGGTGGTACATGAAGGTAGAGGATCTTCTCCCTAGTAAATATCGTGATAAAGCATCCGAATATGAAAAGGGAACTGACACAATGGATGTATGGTTCGACTCAGGCTCTTCTTGGGCTGCAGTTTTAGGAAAAAGAAAAAGCCTTAGTCTCCCTGCAGACTTGTACCTTGAAGGTATGGATCAGCATCGTGGGTGGTTCCAGAGTTCTTTGTTAACAAGTGTTGCTACAAAAGGAAAGGCTCCATATTCCAGTGTCATAACACATGGATTTGTATTGGATGAGAAGGGTTTAAAGATGAGCAAATCTCAGGGTAACGTTGTAGACCCAAGAACTGTGATTGAAGGAGGGAAGAACCAAAAGGATGGCTATGGAGCTGATGTTCTGCGTCTCTGGGTTTCCAGCATAGATTATACAGGCGATGTTATGATAGGTGCTCAAGTTCTCCGTCAAATGTCAGATATATATAGGAAGTTACGAGGAACATTGAGATACCTTTTGGGAAATCTGCATGATTGGCATGCTGATAATGCTATTTCATACCACGATCTTCCCATGATTGATCAGCATGCACTGTTTCAACTTGAAAATTTTGTAAACAACAGCAGAGAGTGCTATGAAAGTTATCAGTTCTTTAAGATATTTCAGATCATACAGCGGTTTGTCATTGTTGACCTATCAAATTTCTACTTTGATATTGCCAAAGATCGTTTATACGTTGGGGGCACGACAAGTTTTACTAGGAGAAGTTGTCAGACGGTTCTTGCAGAACTGCTCCTTTCTATTGTGAGAGTGATTGCTCCAATATTGCCTCATTTGGCTGAGGATGTATGGTTAAATCTTCCATTCCAGTACACTGACAAAAATGGCTCTGTTGCGGAATTTGTTTTTGAATCAAGATGGCCAGCTTCGAACGAAACATGGCTCTCTCTTTCTAAAGAGGAAACTGATTTCTGGACTAAAATTCTTGAGCTGAGAACTGAGGTGAATAAAGTGTTAGAGATTGCTCGTACAACAAAGTTAATTGGCTCTAGCTTAGATGCCAAGGTCTACCTCCATACATCGGATTCTGGCCTGGCCTCAAGATTGGTTCAAATGTCTGCAGCCAAGAACGATGCAGACACATTGAATCGGATATTCATAACTTCCCAGGCAGAGGTTCTTCCATCCTTAGAAGATGACTGGATTGCGAATATACCACATAAAGGAGAATGTCAAGTTGACGAAAGCATTAAAGTTTGGATTGGTGTGTCTCGCGCTGAGGGCCTAAAGTGTGAAAGATGCTGGAACTATTCACCTCAGGTTGGTTCCTTTCCAGACCACAGCACTCTTTGCAGCCGCTGCTATGATGTGGTTGATATTCAACAAGCTGCAGCTGAAGCTGTTGTCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 zf-FPG_IleRS

Protein Analysis

975

Amino Acids

110.23

Weight (kDa)

6.11

Isoelectric Point (pI)

39.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	12 - 654	3.4e-134	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	41 - 185	5e-13	tRNA synthetases class I (M)
Anticodon_1	PF08264	699 - 862	4.9e-29	Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS	PF06827	933 - 962	4.3e-08	Zinc finger found in FPG and IleRS

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49030 AT5G49030 AT5G49030
fragaria_vesca	FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060
malus_domestica	MD13G1168400.v1.1 MD16G1168800.v1.1
prunus_persica	Prupe.1G136500_v2.0.a1 Prupe.1G136500_v2.0.a1
pyrus_communis	pycom13g14400 pycom16g14210
rosa_chinensis	RchiOBHm_Chr4g0422271
rosa_laevigata	RLG00000007623
rosa_multiflora	Rmu_sc0013245.1_g000018
rosa_roxburghii	Rroxscaffold_5G00364380
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238300 Rh4BG241400 Rh4CG253300 Rh4DG237000
rosa_wichuraiana	Rw4G020620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	192, 210
AarI	CACCTGC	1 cut(s)	784
Acc36I	ACCTGC	2 cut(s)	784, 924
AccBSI	CCGCTC	1 cut(s)	1395
AccI	GTMKAC	2 cut(s)	1857, 2583
AccII	CGCG	3 cut(s)	704, 1414, 2790
AccIII	TCCGGA	1 cut(s)	559
AciI	CCGC	8 cut(s)	591, 702, 704, 1393, 1414, 2191, 2411, 2872
AclI	AACGTT	1 cut(s)	1851
AclWI	GGATC	4 cut(s)	306, 419, 1566, 1722
AcoI	YGGCCR	3 cut(s)	148, 660, 2435
AcsI	RAATTY	6 cut(s)	10, 1130, 2122, 2215, 2414, 2496
AcuI	CTGAAG	3 cut(s)	20, 831, 905
AfaI	GTAC	7 cut(s)	125, 747, 1149, 1544, 1700, 2387, 2545
AfiI	CCNNNNNNNGG	8 cut(s)	541, 623, 1037, 1465, 2596, 2689, 2738, 2837
AflII	CTTAAG	1 cut(s)	1064
AjnI	CCWGG	5 cut(s)	226, 680, 1030, 2607, 2682
AloI	GAACNNNNNNTCC	4 cut(s)	1613, 1645, 1899, 1931
Alw21I	GWGCWC	2 cut(s)	613, 1972
Alw26I	GTCTC	5 cut(s)	760, 1481, 1688, 1928, 2790
Alw44I	GTGCAC	1 cut(s)	609
AlwI	GGATC	4 cut(s)	306, 419, 1566, 1722
AlwNI	CAGNNNCTG	3 cut(s)	545, 1031, 2874
Aor13HI	TCCGGA	1 cut(s)	559
AoxI	GGCC	8 cut(s)	148, 660, 880, 1060, 2435, 2605, 2610, 2797
ApaLI	GTGCAC	1 cut(s)	609
ApeKI	GCWGC	9 cut(s)	287, 329, 440, 1652, 2636, 2868, 2874, 2903, 2906
ApoI	RAATTY	6 cut(s)	10, 1130, 2122, 2215, 2414, 2496
AseI	ATTAAT	1 cut(s)	183
Asp700I	GAANNNNTTC	2 cut(s)	830, 1305
AspLEI	GCGC	1 cut(s)	2792
AspS9I	GGNCC	5 cut(s)	116, 469, 880, 1018, 2797
AsuHPI	GGTGA	5 cut(s)	146, 765, 1445, 2530, 2820
AsuII	TTCGAA	1 cut(s)	2444
AvaII	GGWCC	3 cut(s)	116, 469, 1018
AxyI	CCTNAGG	1 cut(s)	2832
BaeGI	GKGCMC	2 cut(s)	613, 2261
BaeI	ACNNNNGTAYC	2 cut(s)	1214, 1247
BalI	TGGCCA	2 cut(s)	662, 2437
BarI	GAAGNNNNNNTAC	2 cut(s)	1545, 1577
BauI	CACGAG	1 cut(s)	21
Bbv12I	GWGCWC	2 cut(s)	613, 1972
BbvCI	CCTCAGC	2 cut(s)	2355, 2793
BbvI	GCAGC	9 cut(s)	274, 341, 427, 1639, 2648, 2861, 2880, 2890, 2918
BccI	CCATC	9 cut(s)	107, 358, 460, 951, 1097, 1151, 1892, 2427, 2707
BciT130I	CCWGG	5 cut(s)	228, 682, 1032, 2609, 2684
BciVI	GTATCC	3 cut(s)	567, 654, 1242
BclI	TGATCA	4 cut(s)	280, 1203, 1495, 2095
BcoDI	GTCTC	5 cut(s)	760, 1481, 1688, 1928, 2790
BfaI	CTAG	5 cut(s)	866, 1389, 1569, 2274, 2565
BfmI	CTRYAG	6 cut(s)	487, 931, 1653, 1689, 2634, 2904
BfrI	CTTAAG	1 cut(s)	1064
BfuAI	ACCTGC	2 cut(s)	784, 924
BfuI	GTATCC	3 cut(s)	567, 654, 1242
BglII	AGATCT	2 cut(s)	850, 919
Bme1390I	CCNGG	5 cut(s)	228, 682, 1032, 2609, 2684
Bme18I	GGWCC	3 cut(s)	116, 469, 1018
BmgT120I	GGNCC	5 cut(s)	116, 469, 880, 1018, 2797
BmiI	GGNNCC	5 cut(s)	881, 1470, 1733, 1776, 2842
BmrFI	CCNGG	5 cut(s)	228, 682, 1032, 2609, 2684
BmsI	GCATC	9 cut(s)	591, 1301, 1315, 1522, 1598, 1729, 2563, 2638, 2804
BplI	GAGNNNNNCTC	4 cut(s)	598, 630, 1825, 1857
BpmI	CTGGAG	3 cut(s)	532, 1074, 1442
Bpu10I	CCTNAGC	3 cut(s)	1356, 2355, 2793
Bpu14I	TTCGAA	1 cut(s)	2444
BpuEI	CTTGAG	5 cut(s)	797, 944, 1956, 2522, 2599
BsaBI	GATNNNNATC	1 cut(s)	1719
BsaJI	CCNNGG	4 cut(s)	680, 702, 2577, 2682
BsaWI	WCCGGW	1 cut(s)	559
BsaXI	ACNNNNNCTCC	6 cut(s)	661, 691, 1899, 1929, 2269, 2299
Bsc4I	CCNNNNNNNGG	8 cut(s)	541, 623, 1037, 1465, 2596, 2689, 2738, 2837
Bse21I	CCTNAGG	1 cut(s)	2832
Bse3DI	GCAATG	3 cut(s)	1289, 1342, 1386
Bse8I	GATNNNNATC	1 cut(s)	1719
BseAI	TCCGGA	1 cut(s)	559
BseBI	CCWGG	5 cut(s)	228, 682, 1032, 2609, 2684
BseDI	CCNNGG	4 cut(s)	680, 702, 2577, 2682
BseGI	GGATG	9 cut(s)	943, 1192, 1330, 1589, 1627, 1819, 1903, 2365, 2699
BseJI	GATNNNNATC	1 cut(s)	1719
BseLI	CCNNNNNNNGG	8 cut(s)	541, 623, 1037, 1465, 2596, 2689, 2738, 2837
BseMI	GCAATG	3 cut(s)	1289, 1342, 1386
BseRI	GAGGAG	2 cut(s)	996, 1499
BseSI	GKGCMC	2 cut(s)	613, 2261
BseXI	GCAGC	9 cut(s)	274, 341, 427, 1639, 2648, 2861, 2880, 2890, 2918
BsgI	GTGCAG	1 cut(s)	125
Bsh1236I	CGCG	3 cut(s)	704, 1414, 2790
BshFI	GGCC	8 cut(s)	150, 662, 882, 1062, 2437, 2607, 2612, 2799
BsiHKAI	GWGCWC	2 cut(s)	613, 1972
BsiSI	CCGG	2 cut(s)	560, 780
BsiWI	CGTACG	1 cut(s)	123
BslFI	GGGAC	1 cut(s)	501
BslI	CCNNNNNNNGG	8 cut(s)	541, 623, 1037, 1465, 2596, 2689, 2738, 2837
BsmAI	GTCTC	5 cut(s)	760, 1481, 1688, 1928, 2790
BsmBI	CGTCTC	1 cut(s)	1928
BsmFI	GGGAC	1 cut(s)	501
BsmI	GAATGC	2 cut(s)	721, 968
BsnI	GGCC	8 cut(s)	150, 662, 882, 1062, 2437, 2607, 2612, 2799
Bsp119I	TTCGAA	1 cut(s)	2444
Bsp1286I	GDGCHC	3 cut(s)	613, 1972, 2261
Bsp13I	TCCGGA	1 cut(s)	559
BspACI	CCGC	8 cut(s)	591, 702, 704, 1393, 1414, 2191, 2411, 2872
BspANI	GGCC	8 cut(s)	150, 662, 882, 1062, 2437, 2607, 2612, 2799
BspEI	TCCGGA	1 cut(s)	559
BspFNI	CGCG	3 cut(s)	704, 1414, 2790
BspHI	TCATGA	1 cut(s)	109
BspLI	GGNNCC	5 cut(s)	881, 1470, 1733, 1776, 2842
BspMAI	CTGCAG	5 cut(s)	935, 1657, 1693, 2638, 2908
BspMI	ACCTGC	2 cut(s)	784, 924
BspPI	GGATC	4 cut(s)	306, 419, 1566, 1722
BspQI	GCTCTTC	1 cut(s)	1648
BspT104I	TTCGAA	1 cut(s)	2444
BspTI	CTTAAG	1 cut(s)	1064
BsrBI	CCGCTC	1 cut(s)	1395
BsrDI	GCAATG	3 cut(s)	1289, 1342, 1386
BssECI	CCNNGG	4 cut(s)	680, 702, 2577, 2682
BssSI	CACGAG	1 cut(s)	21
BssT1I	CCWWGG	1 cut(s)	2577
Bst2BI	CACGAG	1 cut(s)	21
Bst2UI	CCWGG	5 cut(s)	228, 682, 1032, 2609, 2684
Bst4CI	ACNGT	5 cut(s)	352, 750, 1870, 2109, 2293
Bst6I	CTCTTC	1 cut(s)	1648
BstAFI	CTTAAG	1 cut(s)	1064
BstAPI	GCANNNNNTGC	4 cut(s)	716, 2145, 2865, 2874
BstBI	TTCGAA	1 cut(s)	2444
BstC8I	GCNNGC	5 cut(s)	791, 801, 2055, 2103, 2439
BstDSI	CCRYGG	1 cut(s)	702
BstENI	CCTNNNNNAGG	1 cut(s)	539
BstF5I	GGATG	9 cut(s)	943, 1192, 1330, 1589, 1627, 1819, 1903, 2365, 2699
BstFNI	CGCG	3 cut(s)	704, 1414, 2790
BstHHI	GCGC	1 cut(s)	2792
BstMAI	GTCTC	5 cut(s)	760, 1481, 1688, 1928, 2790
BstNI	CCWGG	5 cut(s)	228, 682, 1032, 2609, 2684
BstNSI	RCATGY	2 cut(s)	2057, 2105
BstSCI	CCNGG	5 cut(s)	226, 680, 1030, 2607, 2682
BstSFI	CTRYAG	6 cut(s)	487, 931, 1653, 1689, 2634, 2904
BstSLI	GKGCMC	2 cut(s)	613, 2261
BstUI	CGCG	3 cut(s)	704, 1414, 2790
BstV1I	GCAGC	9 cut(s)	274, 341, 427, 1639, 2648, 2861, 2880, 2890, 2918
BstX2I	RGATCY	4 cut(s)	298, 850, 919, 1558
BstXI	CCANNNNNNTGG	3 cut(s)	510, 1242, 1898
BstYI	RGATCY	4 cut(s)	298, 850, 919, 1558
Bsu36I	CCTNAGG	1 cut(s)	2832
BsuI	GTATCC	3 cut(s)	567, 654, 1242
BsuRI	GGCC	8 cut(s)	150, 662, 882, 1062, 2437, 2607, 2612, 2799
BtgI	CCRYGG	1 cut(s)	702
BtgZI	GCGATG	1 cut(s)	1968
BtsCI	GGATG	9 cut(s)	943, 1192, 1330, 1589, 1627, 1819, 1903, 2365, 2699
BtsI	GCAGTG	1 cut(s)	717
BtsIMutI	CAGTG	8 cut(s)	508, 717, 1056, 1199, 1302, 1792, 2105, 2388
BveI	ACCTGC	2 cut(s)	784, 924
Cac8I	GCNNGC	5 cut(s)	791, 801, 2055, 2103, 2439
CaiI	CAGNNNCTG	3 cut(s)	545, 1031, 2874
CciI	TCATGA	1 cut(s)	109
CfoI	GCGC	1 cut(s)	2792
Cfr13I	GGNCC	5 cut(s)	116, 469, 880, 1018, 2797
Cfr42I	CCGCGG	1 cut(s)	705
CseI	GACGC	1 cut(s)	1910
Csp6I	GTAC	7 cut(s)	124, 746, 1148, 1543, 1699, 2386, 2544
CviQI	GTAC	7 cut(s)	124, 746, 1148, 1543, 1699, 2386, 2544
DraI	TTTAAA	1 cut(s)	1827
EaeI	YGGCCR	3 cut(s)	148, 660, 2435
Eam1104I	CTCTTC	1 cut(s)	1648
EarI	CTCTTC	1 cut(s)	1648
Eco130I	CCWWGG	1 cut(s)	2577
Eco47I	GGWCC	3 cut(s)	116, 469, 1018
Eco57I	CTGAAG	3 cut(s)	20, 831, 905
Eco81I	CCTNAGG	1 cut(s)	2832
EcoNI	CCTNNNNNAGG	1 cut(s)	539
EcoO109I	RGGNCCY	2 cut(s)	880, 2797
EcoRI	GAATTC	1 cut(s)	10
EcoRII	CCWGG	5 cut(s)	226, 680, 1030, 2607, 2682
EcoT14I	CCWWGG	1 cut(s)	2577
EcoT22I	ATGCAT	1 cut(s)	1537
ErhI	CCWWGG	1 cut(s)	2577
Esp3I	CGTCTC	1 cut(s)	1928
FaqI	GGGAC	1 cut(s)	501
FauNDI	CATATG	1 cut(s)	1237
FbaI	TGATCA	4 cut(s)	280, 1203, 1495, 2095
FblI	GTMKAC	2 cut(s)	1857, 2583
FokI	GGATG	9 cut(s)	930, 1199, 1337, 1576, 1634, 1826, 1910, 2372, 2686
FspBI	CTAG	5 cut(s)	866, 1389, 1569, 2274, 2565
GlaI	GCGC	1 cut(s)	2791
GsuI	CTGGAG	3 cut(s)	532, 1074, 1442
HaeIII	GGCC	8 cut(s)	150, 662, 882, 1062, 2437, 2607, 2612, 2799
HapII	CCGG	2 cut(s)	560, 780
HgaI	GACGC	1 cut(s)	1910
HhaI	GCGC	1 cut(s)	2792
Hin6I	GCGC	1 cut(s)	2790
HinP1I	GCGC	1 cut(s)	2790
HincII	GTYRAC	3 cut(s)	1749, 2206, 2755
HindII	GTYRAC	3 cut(s)	1749, 2206, 2755
HindIII	AAGCTT	3 cut(s)	194, 722, 887
HpaI	GTTAAC	1 cut(s)	1749
HpaII	CCGG	2 cut(s)	560, 780
HphI	GGTGA	5 cut(s)	146, 765, 1445, 2530, 2820
HpyAV	CCTTC	8 cut(s)	166, 319, 482, 1295, 1543, 1700, 1813, 1871
HpyCH4III	ACNGT	5 cut(s)	352, 750, 1870, 2109, 2293
HpyCH4IV	ACGT	2 cut(s)	1851, 2250
HpySE526I	ACGT	2 cut(s)	1851, 2250
HspAI	GCGC	1 cut(s)	2790
Kpn2I	TCCGGA	1 cut(s)	559
Ksp22I	TGATCA	4 cut(s)	280, 1203, 1495, 2095
KspAI	GTTAAC	1 cut(s)	1749
KspI	CCGCGG	1 cut(s)	705
LguI	GCTCTTC	1 cut(s)	1648
LmnI	GCTCC	6 cut(s)	579, 1033, 1780, 1907, 2313, 2338
Lsp1109I	GCAGC	9 cut(s)	274, 341, 427, 1639, 2648, 2861, 2880, 2890, 2918
LweI	GCATC	9 cut(s)	591, 1301, 1315, 1522, 1598, 1729, 2563, 2638, 2804
MaeI	CTAG	5 cut(s)	866, 1389, 1569, 2274, 2565
MaeII	ACGT	2 cut(s)	1851, 2250
MaeIII	GTNAC	4 cut(s)	1054, 1197, 1847, 2007
MbiI	CCGCTC	1 cut(s)	1395
MfeI	CAATTG	2 cut(s)	252, 1329
MflI	RGATCY	4 cut(s)	298, 850, 919, 1558
MhlI	GDGCHC	3 cut(s)	613, 1972, 2261
MlsI	TGGCCA	2 cut(s)	662, 2437
MluNI	TGGCCA	2 cut(s)	662, 2437
MlyI	GAGTC	6 cut(s)	412, 523, 574, 670, 1010, 1628
MmeI	TCCRAC	2 cut(s)	531, 641
Mox20I	TGGCCA	2 cut(s)	662, 2437
Mph1103I	ATGCAT	1 cut(s)	1537
MroI	TCCGGA	1 cut(s)	559
MroXI	GAANNNNTTC	2 cut(s)	830, 1305
MscI	TGGCCA	2 cut(s)	662, 2437
Msp20I	TGGCCA	2 cut(s)	662, 2437
MspA1I	CMGCKG	4 cut(s)	704, 2191, 2874, 2909
MspCI	CTTAAG	1 cut(s)	1064
MspI	CCGG	2 cut(s)	560, 780
MspR9I	CCNGG	5 cut(s)	228, 682, 1032, 2609, 2684
MunI	CAATTG	2 cut(s)	252, 1329
Mva1269I	GAATGC	2 cut(s)	721, 968
MvaI	CCWGG	5 cut(s)	228, 682, 1032, 2609, 2684
MvnI	CGCG	3 cut(s)	704, 1414, 2790
NdeI	CATATG	1 cut(s)	1237
NlaIV	GGNNCC	5 cut(s)	881, 1470, 1733, 1776, 2842
NmuCI	GTSAC	2 cut(s)	1054, 1197
NsiI	ATGCAT	1 cut(s)	1537
NspI	RCATGY	2 cut(s)	2057, 2105
NspV	TTCGAA	1 cut(s)	2444
PaeI	GCATGC	2 cut(s)	2057, 2105
PagI	TCATGA	1 cut(s)	109
PaqCI	CACCTGC	1 cut(s)	784
PciSI	GCTCTTC	1 cut(s)	1648
PctI	GAATGC	2 cut(s)	721, 968
PdmI	GAANNNNTTC	2 cut(s)	830, 1305
PfeI	GAWTC	6 cut(s)	368, 1214, 1348, 2426, 2600, 2662
Pfl23II	CGTACG	1 cut(s)	123
PflFI	GACNNNGTC	1 cut(s)	1038
PfoI	TCCNGGA	1 cut(s)	1030
PleI	GAGTC	6 cut(s)	412, 522, 573, 670, 1009, 1628
PpsI	GAGTC	6 cut(s)	412, 522, 573, 670, 1009, 1628
PshBI	ATTAAT	1 cut(s)	183
PsiI	TTATAA	2 cut(s)	192, 210
Psp1406I	AACGTT	1 cut(s)	1851
Psp6I	CCWGG	5 cut(s)	226, 680, 1030, 2607, 2682
PspGI	CCWGG	5 cut(s)	226, 680, 1030, 2607, 2682
PspLI	CGTACG	1 cut(s)	123
PspN4I	GGNNCC	5 cut(s)	881, 1470, 1733, 1776, 2842
PspPI	GGNCC	5 cut(s)	116, 469, 880, 1018, 2797
PstI	CTGCAG	5 cut(s)	935, 1657, 1693, 2638, 2908
PstNI	CAGNNNCTG	3 cut(s)	545, 1031, 2874
PsuI	RGATCY	4 cut(s)	298, 850, 919, 1558
PsyI	GACNNNGTC	1 cut(s)	1038
PvuII	CAGCTG	1 cut(s)	2909
RsaI	GTAC	7 cut(s)	125, 747, 1149, 1544, 1700, 2387, 2545
RsaNI	GTAC	7 cut(s)	124, 746, 1148, 1543, 1699, 2386, 2544
SacII	CCGCGG	1 cut(s)	705
SapI	GCTCTTC	1 cut(s)	1648
Sau96I	GGNCC	5 cut(s)	116, 469, 880, 1018, 2797
SchI	GAGTC	6 cut(s)	412, 523, 574, 670, 1010, 1628
ScrFI	CCNGG	5 cut(s)	228, 682, 1032, 2609, 2684
SduI	GDGCHC	3 cut(s)	613, 1972, 2261
SfaNI	GCATC	9 cut(s)	591, 1301, 1315, 1522, 1598, 1729, 2563, 2638, 2804
SfcI	CTRYAG	6 cut(s)	487, 931, 1653, 1689, 2634, 2904
Sfr303I	CCGCGG	1 cut(s)	705
SfuI	TTCGAA	1 cut(s)	2444
SgrBI	CCGCGG	1 cut(s)	705
SinI	GGWCC	3 cut(s)	116, 469, 1018
SmlI	CTYRAG	6 cut(s)	812, 923, 1064, 1971, 2501, 2614
SmoI	CTYRAG	6 cut(s)	812, 923, 1064, 1971, 2501, 2614
SphI	GCATGC	2 cut(s)	2057, 2105
SsiI	CCGC	8 cut(s)	591, 702, 704, 1393, 1414, 2191, 2411, 2872
SspI	AATATT	3 cut(s)	951, 1165, 2340
SspMI	CTAG	5 cut(s)	866, 1389, 1569, 2274, 2565
StyD4I	CCNGG	5 cut(s)	226, 680, 1030, 2607, 2682
StyI	CCWWGG	1 cut(s)	2577
TaaI	ACNGT	5 cut(s)	352, 750, 1870, 2109, 2293
TaiI	ACGT	2 cut(s)	1854, 2253
TaqI	TCGA	3 cut(s)	292, 1632, 2444
TatI	WGTACW	3 cut(s)	745, 1147, 2385
TauI	GCSGC	4 cut(s)	704, 1395, 1417, 2874
TfiI	GAWTC	6 cut(s)	368, 1214, 1348, 2426, 2600, 2662
TscAI	CASTG	8 cut(s)	515, 717, 1063, 1206, 1302, 1792, 2112, 2395
TseFI	GTSAC	2 cut(s)	1054, 1197
TseI	GCWGC	9 cut(s)	287, 329, 440, 1652, 2636, 2868, 2874, 2903, 2906
Tsp45I	GTSAC	2 cut(s)	1054, 1197
TspGWI	ACGGA	2 cut(s)	111, 1970
TspRI	CASTG	8 cut(s)	515, 717, 1063, 1206, 1302, 1792, 2112, 2395
Tth111I	GACNNNGTC	1 cut(s)	1038
Vha464I	CTTAAG	1 cut(s)	1064
VneI	GTGCAC	1 cut(s)	609
VpaK11BI	GGWCC	3 cut(s)	116, 469, 1018
VspI	ATTAAT	1 cut(s)	183
XagI	CCTNNNNNAGG	1 cut(s)	539
XapI	RAATTY	6 cut(s)	10, 1130, 2122, 2215, 2414, 2496
XbaI	TCTAGA	1 cut(s)	865
XceI	RCATGY	2 cut(s)	2057, 2105
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1028
XmiI	GTMKAC	2 cut(s)	1857, 2583
XmnI	GAANNNNTTC	2 cut(s)	830, 1305
XspI	CTAG	5 cut(s)	866, 1389, 1569, 2274, 2565
Zsp2I	ATGCAT	1 cut(s)	1537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.