Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007310

Physical Location & Seq

Genomic Coordinates Forward (+)

14029371 .. 14040464

Gene Structure 11094 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1168800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3282 bp

ATGAACTTCAAATCCTTCGCAGCCCACTCTTGGGCGCTCCAACCCAGGGAAGCCGCCATGGCTGTGATTCAGACCTCCCCCTACAGGGTTCTGTCACAAAGAACTTGCTCATCTTTTAGGAGCACAGCTTCTGTTGGCTTATTTTATTTCAGAGACAGGTCTTCAGTTAAAGTTTTCTCTCTCTTTAACATGGCGCACTACTCTGCTTTTTCCAATGATGAGTTTGGTTCTTCCTCAAAGCGAAGATCTCGAGGACCTGTTATGGCCGCAAAGAAAGTAGCTGAAGGGAAAAAGCAAGAAGAGGGAAAATACAAGCACACGGTTGAACTGCCAAAGACATCATTTAGCATGAGAGCGAACTCTTTAACACGAGAGCCTGAAATCCAAAAAATATGGGATGACAATCAAGTTTTCAAGAGAGTTGTTGGTAAAAATACAGGGGAAAATTTCATTCTTCATGATGGTCCTCCGTATGCCAATGGAGATCTTCACATAGGCCATGCTTTAAATAAGATTTTAAAGGATTTTATCAATCGTTATAAGCTTCTTCAAAATTATAAAGTTCATTATGTGCCTGGTTGGGATTGTCATGGCTTACCAATTGAGTTGAAAGTTCTTCAATCACTTGATCAAGCTGCTCGAAAGGATCTAACACCAATAAAGTTGAGAGCAAAGGCAGCCAAATTTGCCAAGCAAACCGTTAAAACTCAGATGGAATCATTTAAGCGTTATGGAGTGTGGGCAGATTGGAATAATCCCTACCTCACTCTTGATCCAGAATATGAAGCTGCTCAGATAGAAGTGTTTGGCCAGATGGTCCTTCAAGGTTTTATCTACAGAGGCAAGAAACCAGTTCACTGGAGCCCCTCATCACGAACTGCTCTTGCAGAGGCTGAGTTGGAGTATCCGGAGGGGCATGTTTCGAGAAGCATATACGCCATTTTCAAATTGGTCAGTGCCTCTCCTACTTCAGGTGGCTTATTAAATGAGTATTTCCCAGATGTGTGCTTGGCCATTTGGACAACTACTCCCTGGACTATTCCAGCCAATGCTGCTGTTGCAGTGAATGCCAAGCTTTTATATGCCATTGTTGAAGTACAGTCACTCCCAGAAGATTCTTTATCTGATGGAAATAAAAAGGGAAGGCCTGGAAATTTTTTGAAGGAAGAAAAGAAGCCTTTCCTAATTGTCGCTTCAGATCTTGTGCCAGTGTTAGAAGCAAAATGGCGTGTGAAGCTTCTTGTTAGGAAAAGGGTGTCAGGTTCAGATCTGGAAAACTGCAGGTATGTCCATCCGATATTTAATAGGGAATGCCCGGTTGTCATTGGAGGAGATTATATTACAACGGACTCAGGAACAGGACTGGTCCATACAGCTCCTGGACATGGTCAGGAGGATTATGTCACTGGCCAGAAGTACGGACTACCTATGCTTTCTCCTGTAGATGATGATGGAAAGTTCACTGAAGAAGCTGGAAAGTTTTGTGGGCTTGATGTGCTTGCGGATGGTAATATTGCTGTGGTGAAATACTTGGATGAACATTTATCAATTATCATGGAAGAATCCTACAATCATAAGTATCCATACGATTGGAGAACAAAAAAGCCAACAATATTTAGGGCAACTGAGCAATGGTTTGCTTCAGTAGAAGGGTTTCGCGACGCTGTTATGGATGCAATTGGTCATGTAAAATGGATTCCACCTAAGGCAGAAAACAGAATATCTGCAATGACTTCTAGCCGCTCTGATTGGTGTATATCGCGGCAGAGGACATGGGGTGTTCCAATTCCAGTCTTTTATCATGTCGATTCAAAGGAACCTCTGATGAATGAAGAGACTATTGAGTATATCAAGTCTATAATATCCGAAAAGGGTAGTGATGCGTGGTGGTACATGAAGGTGGAGGATCTCCTCCCCGATAAATATCGTGATAAAGCATCTGAATATGAAAAGGGGACTGATACAATGGATGTATGGTTTGATTCAGGCTCTTCTTGGGCTGCAGTATTGGGAAAAAGAAATAGCCATAGTCTTCCGGCGGATTTGTACCTTGAGGGTATGGATCAGCATCGTGGGTGGTTCCAGAGTTCTTTGTTAACAAGTATTGCTACTAAAGGAAAGGCTCCATATTCCAGTGTTATAACACATGGATTTGTATTGGATGAGAAAGGTTTGAAAATGAGCAAATCTTTGGGTAACGTAGTAGATCCACGGACTGTGATTGAAGGAGGGAAGAACCAAAAGGATGGCTATGGAGCTGATGTCCTGCGTCTCTGGGTTTCTAGTGTAGATTATACAGGCGATGTAACAATTGGCCCCCAAATTCTCCGTCAAATGTCAGATATTTATAGGAAGATGCGAGGAACATTGAGATACCTTTTGGGAAATCTTCATGATTGGAATGCTGATAATGCTATCGCATACCATGATCTTCCCATGATTGATCAGTACGTGCTGTTTCAGCTCGAAAATTTTGTGAAGAACAGTAGAGAGTGCTATGAAAACTATCAATTCTTCAAGATATTTCAGATCATACAGCGGTTTGTCGTTGTTGACCTCTCAAATTTCTATTTTGATGTCGCCAAAGATCGCCTTTATGTTGGGGGCACAACAAGTTTTACTAGGAGAAGTTGTCAAACAGTTCTTTCAGCACTTCTCCTATCTGTAGTGAGAGTGATTGCTCCAATATTACCTCATTTGGCTGAAGATGTATGGCAAAACCTTCCGTTCCAGTACACTGATGAAGGTGGCTCTGCTGCTGAATTTGTTTTTGAATCAAGATGGCCAGCTTTGAACAACACATGGCTCTCTCTTTCGAAAGAGGAAACTGATTTCTGGGAAAAAGTTCTTGAGTTGAGAACTGAGGTGAATAAAGTTCTGGAGGTTGCTCGTACGGAAAAGCTAATTGGTTCTAGTTTAGATGCCAAGGTCTACCTCCACACTTCTGATTCCAGTCTGGCCTCTAGATTAGTTGAAATTTCTGCAGCCGACAATGATGCAGACACATTGAATCGGATTTTCATCACATCTCAAGCAGAGGTTCTCCCGTCCTTGGAGGAGGAGCTGATTGCGAATATACCGCACAAAGGAGAGTACCTCATCGAAGAAAACATCAAAGTCTGGATTGGAGTGTCTCGCGCTGAGGGCCTAAAGTGCGAAAGATGCTGGAATTATTCACCTCAAGTTGGCTCCTTTCCAGAGCACAACACTCTTTGCAGCCGCTGTTATAATGTGGTTGATATTCAACAGTCTCAATCTCCAGCTGTAGCTGTGGTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 zf-FPG_IleRS

Protein Analysis

1094

Amino Acids

123.76

Weight (kDa)

6.54

Isoelectric Point (pI)

41.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	128 - 771	4.5e-134	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	157 - 301	1.3e-12	tRNA synthetases class I (M)
Anticodon_1	PF08264	815 - 978	1.2e-30	Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS	PF06827	1049 - 1078	8.3e-08	Zinc finger found in FPG and IleRS

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49030 AT5G49030 AT5G49030
fragaria_vesca	FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060
malus_domestica	MD13G1168400.v1.1 MD16G1168800.v1.1
prunus_persica	Prupe.1G136500_v2.0.a1 Prupe.1G136500_v2.0.a1
pyrus_communis	pycom13g14400 pycom16g14210
rosa_chinensis	RchiOBHm_Chr4g0422271
rosa_laevigata	RLG00000007623
rosa_multiflora	Rmu_sc0013245.1_g000018
rosa_roxburghii	Rroxscaffold_5G00364380
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238300 Rh4BG241400 Rh4CG253300 Rh4DG237000
rosa_wichuraiana	Rw4G020620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	540, 558, 2141, 3228
Acc36I	ACCTGC	1 cut(s)	1272
AccBSI	CCGCTC	1 cut(s)	1743
AccI	GTMKAC	1 cut(s)	2931
AccII	CGCG	3 cut(s)	1659, 1762, 3138
AccIII	TCCGGA	1 cut(s)	907
AciI	CCGC	9 cut(s)	54, 267, 1502, 1741, 1762, 2039, 2539, 3080, 3220
AclWI	GGATC	5 cut(s)	654, 767, 1914, 2070, 2201
AcoI	YGGCCR	5 cut(s)	264, 808, 1011, 1408, 2783
AcsI	RAATTY	8 cut(s)	445, 683, 1153, 2322, 2470, 2563, 2762, 2976
AcuI	CTGAAG	7 cut(s)	147, 303, 954, 1179, 1485, 1626, 2724
AfaI	GTAC	8 cut(s)	1098, 1418, 1892, 2048, 2450, 2735, 2893, 3095
AjnI	CCWGG	5 cut(s)	44, 574, 1031, 1147, 1378
AjuI	GAANNNNNNNTTGG	2 cut(s)	2889, 2921
AloI	GAACNNNNNNTCC	1 cut(s)	28
Alw21I	GWGCWC	2 cut(s)	125, 3204
Alw26I	GTCTC	5 cut(s)	147, 1829, 2276, 3138, 3255
AlwI	GGATC	5 cut(s)	654, 767, 1914, 2070, 2201
AlwNI	CAGNNNCTG	4 cut(s)	131, 893, 1379, 3222
Ama87I	CYCGRG	1 cut(s)	249
Aor13HI	TCCGGA	1 cut(s)	907
ApeKI	GCWGC	9 cut(s)	20, 635, 677, 788, 1052, 2000, 2756, 2984, 3216
ApoI	RAATTY	8 cut(s)	445, 683, 1153, 2322, 2470, 2563, 2762, 2976
ArsI	GACNNNNNNTTYG	2 cut(s)	2314, 2346
Asp700I	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
AspLEI	GCGC	3 cut(s)	37, 196, 3140
AspS9I	GGNCC	6 cut(s)	254, 464, 817, 1366, 2315, 3145
AsuC2I	CCSGG	1 cut(s)	1316
AsuHPI	GGTGA	3 cut(s)	1534, 2878, 3168
AsuII	TTCGAA	1 cut(s)	2816
AvaI	CYCGRG	1 cut(s)	249
AvaII	GGWCC	4 cut(s)	254, 464, 817, 1366
AxyI	CCTNAGG	1 cut(s)	1704
BaeGI	GKGCMC	1 cut(s)	2609
BalI	TGGCCA	4 cut(s)	810, 1013, 1410, 2785
BanII	GRGCYC	1 cut(s)	866
BauI	CACGAG	1 cut(s)	369
BbsI	GAAGAC	2 cut(s)	153, 2024
Bbv12I	GWGCWC	2 cut(s)	125, 3204
BbvCI	CCTCAGC	1 cut(s)	3141
BbvI	GCAGC	9 cut(s)	32, 622, 689, 775, 1039, 1987, 2743, 2996, 3228
BccI	CCATC	9 cut(s)	455, 706, 808, 1121, 1299, 1445, 1499, 2240, 2775
BcgI	CGANNNNNNTGC	2 cut(s)	2978, 3012
BciT130I	CCWGG	5 cut(s)	46, 576, 1033, 1149, 1380
BciVI	GTATCC	2 cut(s)	915, 1590
BclI	TGATCA	2 cut(s)	628, 2443
BcnI	CCSGG	1 cut(s)	1316
BcoDI	GTCTC	5 cut(s)	147, 1829, 2276, 3138, 3255
BfaI	CTAG	5 cut(s)	1737, 2283, 2622, 2913, 2964
BfmI	CTRYAG	8 cut(s)	82, 835, 1279, 1440, 2001, 2664, 2982, 3264
BfoI	RGCGCY	1 cut(s)	38
BfuAI	ACCTGC	1 cut(s)	1272
BfuI	GTATCC	2 cut(s)	915, 1590
BglI	GCCNNNNNGGC	1 cut(s)	59
BglII	AGATCT	4 cut(s)	245, 484, 1198, 1267
Bme1390I	CCNGG	6 cut(s)	46, 576, 1033, 1149, 1316, 1380
Bme18I	GGWCC	4 cut(s)	254, 464, 817, 1366
BmeT110I	CYCGRG	1 cut(s)	249
BmgT120I	GGNCC	6 cut(s)	254, 464, 817, 1366, 2315, 3145
BmiI	GGNNCC	6 cut(s)	863, 1818, 2081, 2124, 2317, 3190
BmrFI	CCNGG	6 cut(s)	46, 576, 1033, 1149, 1316, 1380
BmsI	GCATC	8 cut(s)	1663, 1870, 1946, 2077, 2346, 2911, 2986, 3152
BpiI	GAAGAC	2 cut(s)	153, 2024
BplI	GAGNNNNNCTC	2 cut(s)	3081, 3113
BpmI	CTGGAG	3 cut(s)	880, 2900, 3243
Bpu10I	CCTNAGC	1 cut(s)	3141
Bpu14I	TTCGAA	1 cut(s)	2816
BpuEI	CTTGAG	4 cut(s)	2072, 2870, 3015, 3165
BpuMI	CCSGG	1 cut(s)	1316
BsaAI	YACGTR	1 cut(s)	2452
BsaBI	GATNNNNATC	4 cut(s)	402, 1923, 2067, 3020
BsaJI	CCNNGG	7 cut(s)	44, 45, 57, 1031, 2210, 2925, 3051
BsaWI	WCCGGW	1 cut(s)	907
BsaXI	ACNNNNNCTCC	8 cut(s)	1012, 1042, 1089, 1119, 2247, 2277, 2617, 2647
Bse1I	ACTGG	9 cut(s)	851, 863, 1208, 1368, 1411, 1790, 2133, 2731, 2952
Bse21I	CCTNAGG	1 cut(s)	1704
Bse3DI	GCAATG	2 cut(s)	1637, 1734
Bse8I	GATNNNNATC	4 cut(s)	402, 1923, 2067, 3020
BseAI	TCCGGA	1 cut(s)	907
BseBI	CCWGG	5 cut(s)	46, 576, 1033, 1149, 1380
BseDI	CCNNGG	7 cut(s)	44, 45, 57, 1031, 2210, 2925, 3051
BseGI	GGATG	8 cut(s)	403, 1291, 1510, 1540, 1678, 1975, 2167, 2251
BseJI	GATNNNNATC	4 cut(s)	402, 1923, 2067, 3020
BseMI	GCAATG	2 cut(s)	1637, 1734
BseMII	CTCAG	7 cut(s)	722, 806, 885, 1365, 1617, 2853, 3132
BseNI	ACTGG	9 cut(s)	851, 863, 1208, 1368, 1411, 1790, 2133, 2731, 2952
BseRI	GAGGAG	4 cut(s)	1344, 1901, 3071, 3074
BseSI	GKGCMC	1 cut(s)	2609
BseXI	GCAGC	9 cut(s)	32, 622, 689, 775, 1039, 1987, 2743, 2996, 3228
Bsh1236I	CGCG	3 cut(s)	1659, 1762, 3138
BsiHKAI	GWGCWC	2 cut(s)	125, 3204
BsiHKCI	CYCGRG	1 cut(s)	249
BsiSI	CCGG	3 cut(s)	908, 1316, 2036
BsiWI	CGTACG	1 cut(s)	2891
BslFI	GGGAC	1 cut(s)	1969
BsmAI	GTCTC	5 cut(s)	147, 1829, 2276, 3138, 3255
BsmBI	CGTCTC	1 cut(s)	2276
BsmFI	GGGAC	1 cut(s)	1969
BsmI	GAATGC	3 cut(s)	1072, 1316, 2407
BsoBI	CYCGRG	1 cut(s)	249
Bsp119I	TTCGAA	1 cut(s)	2816
Bsp1286I	GDGCHC	4 cut(s)	125, 866, 2609, 3204
Bsp13I	TCCGGA	1 cut(s)	907
Bsp19I	CCATGG	1 cut(s)	57
Bsp68I	TCGCGA	1 cut(s)	1659
BspACI	CCGC	9 cut(s)	54, 267, 1502, 1741, 1762, 2039, 2539, 3080, 3220
BspCNI	CTCAG	7 cut(s)	721, 805, 886, 1364, 1618, 2854, 3133
BspEI	TCCGGA	1 cut(s)	907
BspFNI	CGCG	3 cut(s)	1659, 1762, 3138
BspHI	TCATGA	2 cut(s)	457, 2392
BspLI	GGNNCC	6 cut(s)	863, 1818, 2081, 2124, 2317, 3190
BspMAI	CTGCAG	3 cut(s)	1283, 2005, 2986
BspMI	ACCTGC	1 cut(s)	1272
BspPI	GGATC	5 cut(s)	654, 767, 1914, 2070, 2201
BspQI	GCTCTTC	1 cut(s)	1996
BspT104I	TTCGAA	1 cut(s)	2816
BsrBI	CCGCTC	1 cut(s)	1743
BsrDI	GCAATG	2 cut(s)	1637, 1734
BsrI	ACTGG	9 cut(s)	851, 863, 1208, 1368, 1411, 1790, 2133, 2731, 2952
BssECI	CCNNGG	7 cut(s)	44, 45, 57, 1031, 2210, 2925, 3051
BssSI	CACGAG	1 cut(s)	369
BssT1I	CCWWGG	3 cut(s)	57, 2925, 3051
Bst2BI	CACGAG	1 cut(s)	369
Bst2UI	CCWGG	5 cut(s)	46, 576, 1033, 1149, 1380
Bst4CI	ACNGT	7 cut(s)	322, 700, 1101, 2218, 2486, 2641, 3249
Bst6I	CTCTTC	3 cut(s)	294, 1827, 1996
BstAPI	GCANNNNNTGC	1 cut(s)	1067
BstBAI	YACGTR	1 cut(s)	2452
BstBI	TTCGAA	1 cut(s)	2816
BstC8I	GCNNGC	2 cut(s)	1500, 2787
BstDEI	CTNAG	8 cut(s)	708, 792, 894, 1351, 1626, 1704, 2862, 3141
BstDSI	CCRYGG	2 cut(s)	57, 2210
BstENI	CCTNNNNNAGG	1 cut(s)	969
BstF5I	GGATG	8 cut(s)	403, 1291, 1510, 1540, 1678, 1975, 2167, 2251
BstFNI	CGCG	3 cut(s)	1659, 1762, 3138
BstH2I	RGCGCY	1 cut(s)	38
BstHHI	GCGC	3 cut(s)	37, 196, 3140
BstMAI	GTCTC	5 cut(s)	147, 1829, 2276, 3138, 3255
BstNI	CCWGG	5 cut(s)	46, 576, 1033, 1149, 1380
BstNSI	RCATGY	1 cut(s)	920
BstSCI	CCNGG	6 cut(s)	44, 574, 1031, 1147, 1314, 1378
BstSFI	CTRYAG	8 cut(s)	82, 835, 1279, 1440, 2001, 2664, 2982, 3264
BstSLI	GKGCMC	1 cut(s)	2609
BstUI	CGCG	3 cut(s)	1659, 1762, 3138
BstV1I	GCAGC	9 cut(s)	32, 622, 689, 775, 1039, 1987, 2743, 2996, 3228
BstV2I	GAAGAC	2 cut(s)	153, 2024
BstX2I	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
BstXI	CCANNNNNNTGG	3 cut(s)	858, 1590, 2246
BstYI	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
Bsu36I	CCTNAGG	1 cut(s)	1704
BsuI	GTATCC	2 cut(s)	915, 1590
BtgI	CCRYGG	2 cut(s)	57, 2210
BtgZI	GCGATG	1 cut(s)	2316
BtsCI	GGATG	8 cut(s)	403, 1291, 1510, 1540, 1678, 1975, 2167, 2251
BtsI	GCAGTG	1 cut(s)	1068
BtsIMutI	CAGTG	8 cut(s)	856, 961, 1068, 1215, 1404, 1461, 2140, 2736
BtuMI	TCGCGA	1 cut(s)	1659
BveI	ACCTGC	1 cut(s)	1272
Cac8I	GCNNGC	2 cut(s)	1500, 2787
CaiI	CAGNNNCTG	4 cut(s)	131, 893, 1379, 3222
CciI	TCATGA	2 cut(s)	457, 2392
CfoI	GCGC	3 cut(s)	37, 196, 3140
Cfr13I	GGNCC	6 cut(s)	254, 464, 817, 1366, 2315, 3145
CseI	GACGC	2 cut(s)	1670, 2258
Csp6I	GTAC	8 cut(s)	1097, 1417, 1891, 2047, 2449, 2734, 2892, 3094
CviQI	GTAC	8 cut(s)	1097, 1417, 1891, 2047, 2449, 2734, 2892, 3094
DdeI	CTNAG	8 cut(s)	708, 792, 894, 1351, 1626, 1704, 2862, 3141
DraI	TTTAAA	2 cut(s)	507, 519
EaeI	YGGCCR	5 cut(s)	264, 808, 1011, 1408, 2783
Eam1104I	CTCTTC	3 cut(s)	294, 1827, 1996
EarI	CTCTTC	3 cut(s)	294, 1827, 1996
EciI	GGCGGA	1 cut(s)	2054
Eco130I	CCWWGG	3 cut(s)	57, 2925, 3051
Eco147I	AGGCCT	1 cut(s)	1147
Eco24I	GRGCYC	1 cut(s)	866
Eco47I	GGWCC	4 cut(s)	254, 464, 817, 1366
Eco57I	CTGAAG	7 cut(s)	147, 303, 954, 1179, 1485, 1626, 2724
Eco81I	CCTNAGG	1 cut(s)	1704
Eco88I	CYCGRG	1 cut(s)	249
EcoNI	CCTNNNNNAGG	1 cut(s)	969
EcoO109I	RGGNCCY	2 cut(s)	254, 3145
EcoRII	CCWGG	5 cut(s)	44, 574, 1031, 1147, 1378
EcoT14I	CCWWGG	3 cut(s)	57, 2925, 3051
EcoT38I	GRGCYC	1 cut(s)	866
ErhI	CCWWGG	3 cut(s)	57, 2925, 3051
Esp3I	CGTCTC	1 cut(s)	2276
FalI	AAGNNNNNCTT	2 cut(s)	2577, 2609
FaqI	GGGAC	1 cut(s)	1969
FbaI	TGATCA	2 cut(s)	628, 2443
FblI	GTMKAC	1 cut(s)	2931
FokI	GGATG	8 cut(s)	410, 1278, 1517, 1547, 1685, 1982, 2174, 2258
FriOI	GRGCYC	1 cut(s)	866
FspBI	CTAG	5 cut(s)	1737, 2283, 2622, 2913, 2964
GlaI	GCGC	3 cut(s)	36, 195, 3139
GsuI	CTGGAG	3 cut(s)	880, 2900, 3243
HaeII	RGCGCY	1 cut(s)	38
HapII	CCGG	3 cut(s)	908, 1316, 2036
HgaI	GACGC	2 cut(s)	1670, 2258
HhaI	GCGC	3 cut(s)	37, 196, 3140
Hin6I	GCGC	3 cut(s)	35, 194, 3138
HinP1I	GCGC	3 cut(s)	35, 194, 3138
HincII	GTYRAC	2 cut(s)	2097, 2554
HindII	GTYRAC	2 cut(s)	2097, 2554
HindIII	AAGCTT	3 cut(s)	542, 1073, 1235
HpaI	GTTAAC	1 cut(s)	2097
HpaII	CCGG	3 cut(s)	908, 1316, 2036
HphI	GGTGA	3 cut(s)	1534, 2878, 3168
Hpy166II	GTNNAC	6 cut(s)	856, 1461, 2097, 2554, 2736, 2932
Hpy8I	GTNNAC	6 cut(s)	856, 1461, 2097, 2554, 2736, 2932
Hpy99I	CGWCG	1 cut(s)	1664
HpyCH4III	ACNGT	7 cut(s)	322, 700, 1101, 2218, 2486, 2641, 3249
HpyCH4IV	ACGT	2 cut(s)	2199, 2451
HpyCH4V	TGCA	9 cut(s)	887, 1061, 1281, 1676, 1727, 2003, 2984, 2999, 3216
HpyF3I	CTNAG	8 cut(s)	708, 792, 894, 1351, 1626, 1704, 2862, 3141
HpySE526I	ACGT	2 cut(s)	2199, 2451
HspAI	GCGC	3 cut(s)	35, 194, 3138
Kpn2I	TCCGGA	1 cut(s)	907
Ksp22I	TGATCA	2 cut(s)	628, 2443
KspAI	GTTAAC	1 cut(s)	2097
LguI	GCTCTTC	1 cut(s)	1996
LmnI	GCTCC	9 cut(s)	42, 120, 861, 1381, 2128, 2255, 2686, 3061, 3194
Lsp1109I	GCAGC	9 cut(s)	32, 622, 689, 775, 1039, 1987, 2743, 2996, 3228
LweI	GCATC	8 cut(s)	1663, 1870, 1946, 2077, 2346, 2911, 2986, 3152
MaeI	CTAG	5 cut(s)	1737, 2283, 2622, 2913, 2964
MaeII	ACGT	2 cut(s)	2199, 2451
MaeIII	GTNAC	5 cut(s)	93, 1101, 1402, 2195, 2305
MbiI	CCGCTC	1 cut(s)	1743
MfeI	CAATTG	3 cut(s)	600, 1677, 2310
MflI	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
MhlI	GDGCHC	4 cut(s)	125, 866, 2609, 3204
MlsI	TGGCCA	4 cut(s)	810, 1013, 1410, 2785
MluNI	TGGCCA	4 cut(s)	810, 1013, 1410, 2785
MlyI	GAGTC	1 cut(s)	1343
MmeI	TCCRAC	2 cut(s)	64, 879
Mox20I	TGGCCA	4 cut(s)	810, 1013, 1410, 2785
MroI	TCCGGA	1 cut(s)	907
MroXI	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
MscI	TGGCCA	4 cut(s)	810, 1013, 1410, 2785
MslI	CAYNNNNRTG	2 cut(s)	62, 1898
Msp20I	TGGCCA	4 cut(s)	810, 1013, 1410, 2785
MspA1I	CMGCKG	3 cut(s)	2539, 3222, 3263
MspI	CCGG	3 cut(s)	908, 1316, 2036
MspR9I	CCNGG	6 cut(s)	46, 576, 1033, 1149, 1316, 1380
MunI	CAATTG	3 cut(s)	600, 1677, 2310
Mva1269I	GAATGC	3 cut(s)	1072, 1316, 2407
MvaI	CCWGG	5 cut(s)	46, 576, 1033, 1149, 1380
MvnI	CGCG	3 cut(s)	1659, 1762, 3138
NciI	CCSGG	1 cut(s)	1316
NcoI	CCATGG	1 cut(s)	57
NlaIV	GGNNCC	6 cut(s)	863, 1818, 2081, 2124, 2317, 3190
NmuCI	GTSAC	3 cut(s)	93, 1101, 1402
NruI	TCGCGA	1 cut(s)	1659
NspI	RCATGY	1 cut(s)	920
NspV	TTCGAA	1 cut(s)	2816
PaeR7I	CTCGAG	1 cut(s)	249
PagI	TCATGA	2 cut(s)	457, 2392
PasI	CCCWGGG	1 cut(s)	45
PceI	AGGCCT	1 cut(s)	1147
PciSI	GCTCTTC	1 cut(s)	1996
PctI	GAATGC	3 cut(s)	1072, 1316, 2407
PdmI	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
Pfl23II	CGTACG	1 cut(s)	2891
PflFI	GACNNNGTC	1 cut(s)	1386
PfoI	TCCNGGA	1 cut(s)	1378
PleI	GAGTC	1 cut(s)	1343
PpsI	GAGTC	1 cut(s)	1343
Ppu21I	YACGTR	1 cut(s)	2452
PpuMI	RGGWCCY	1 cut(s)	254
PsiI	TTATAA	4 cut(s)	540, 558, 2141, 3228
Psp5II	RGGWCCY	1 cut(s)	254
Psp6I	CCWGG	5 cut(s)	44, 574, 1031, 1147, 1378
PspGI	CCWGG	5 cut(s)	44, 574, 1031, 1147, 1378
PspLI	CGTACG	1 cut(s)	2891
PspN4I	GGNNCC	6 cut(s)	863, 1818, 2081, 2124, 2317, 3190
PspPI	GGNCC	6 cut(s)	254, 464, 817, 1366, 2315, 3145
PspPPI	RGGWCCY	1 cut(s)	254
PstI	CTGCAG	3 cut(s)	1283, 2005, 2986
PstNI	CAGNNNCTG	4 cut(s)	131, 893, 1379, 3222
PsuI	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
PsyI	GACNNNGTC	1 cut(s)	1386
PvuII	CAGCTG	1 cut(s)	3263
RruI	TCGCGA	1 cut(s)	1659
RsaI	GTAC	8 cut(s)	1098, 1418, 1892, 2048, 2450, 2735, 2893, 3095
RsaNI	GTAC	8 cut(s)	1097, 1417, 1891, 2047, 2449, 2734, 2892, 3094
RseI	CAYNNNNRTG	2 cut(s)	62, 1898
SapI	GCTCTTC	1 cut(s)	1996
Sau96I	GGNCC	6 cut(s)	254, 464, 817, 1366, 2315, 3145
SchI	GAGTC	1 cut(s)	1343
ScrFI	CCNGG	6 cut(s)	46, 576, 1033, 1149, 1316, 1380
SduI	GDGCHC	4 cut(s)	125, 866, 2609, 3204
SfaNI	GCATC	8 cut(s)	1663, 1870, 1946, 2077, 2346, 2911, 2986, 3152
SfcI	CTRYAG	8 cut(s)	82, 835, 1279, 1440, 2001, 2664, 2982, 3264
Sfr274I	CTCGAG	1 cut(s)	249
SfuI	TTCGAA	1 cut(s)	2816
SinI	GGWCC	4 cut(s)	254, 464, 817, 1366
SlaI	CTCGAG	1 cut(s)	249
SmiMI	CAYNNNNRTG	2 cut(s)	62, 1898
SmlI	CTYRAG	5 cut(s)	249, 2051, 2849, 3030, 3180
SmoI	CTYRAG	5 cut(s)	249, 2051, 2849, 3030, 3180
SseBI	AGGCCT	1 cut(s)	1147
SsiI	CCGC	9 cut(s)	54, 267, 1502, 1741, 1762, 2039, 2539, 3080, 3220
SspI	AATATT	3 cut(s)	1513, 1614, 2688
SspMI	CTAG	5 cut(s)	1737, 2283, 2622, 2913, 2964
StuI	AGGCCT	1 cut(s)	1147
StyD4I	CCNGG	6 cut(s)	44, 574, 1031, 1147, 1314, 1378
StyI	CCWWGG	3 cut(s)	57, 2925, 3051
TaaI	ACNGT	7 cut(s)	322, 700, 1101, 2218, 2486, 2641, 3249
TaiI	ACGT	2 cut(s)	2202, 2454
TaqI	TCGA	7 cut(s)	250, 640, 923, 1806, 2466, 2816, 3102
TatI	WGTACW	2 cut(s)	1096, 2733
TauI	GCSGC	5 cut(s)	56, 269, 1743, 1765, 3222
TscAI	CASTG	8 cut(s)	863, 961, 1068, 1215, 1411, 1468, 2140, 2743
TseFI	GTSAC	3 cut(s)	93, 1101, 1402
TseI	GCWGC	9 cut(s)	20, 635, 677, 788, 1052, 2000, 2756, 2984, 3216
Tsp45I	GTSAC	3 cut(s)	93, 1101, 1402
TspGWI	ACGGA	7 cut(s)	459, 1361, 1434, 2227, 2318, 2715, 2909
TspRI	CASTG	8 cut(s)	863, 961, 1068, 1215, 1411, 1468, 2140, 2743
Tth111I	GACNNNGTC	1 cut(s)	1386
VpaK11BI	GGWCC	4 cut(s)	254, 464, 817, 1366
XagI	CCTNNNNNAGG	1 cut(s)	969
XapI	RAATTY	8 cut(s)	445, 683, 1153, 2322, 2470, 2563, 2762, 2976
XbaI	TCTAGA	1 cut(s)	2963
XceI	RCATGY	1 cut(s)	920
XcmI	CCANNNNNNNNNTGG	2 cut(s)	946, 1376
XhoI	CTCGAG	1 cut(s)	249
XmiI	GTMKAC	1 cut(s)	2931
XmnI	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
XspI	CTAG	5 cut(s)	1737, 2283, 2622, 2913, 2964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.