Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007310

Physical Location & Seq

Genomic Coordinates Reverse (-)

19874925 .. 19883297

Gene Structure 8373 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G49030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3282 bp

ATGTCGTCTTTCTTCAAATCCTTCGCGGGAAATCCCAGGGAAGCTGCTGCAATGGCGATGGTTCAATCTTCATCTTACAGAGTTTTGTCAGGTAAAAGTTGTTCAAATTTAAGGAGAAACACGCCGTTGGATTCTTTTCTTGCCAAGGGAAGGTCTTCCGTCAAGGCTTTTTCATTCTTATATGTCTCACGGTTTTCAACTGAACCAAACAATGAGTTTGGTCATTCATCAAAACGAAGGTCTCGTGGGCCTGTTATGGCAGCCAAGAAAGCATCTGAAGGGGAAAAGCAAGAAGATGGAAAGTACAAGCAAACGGTTGATTTGCCAAAGACTGGTTTTGGTATGAGAGCGAATTCTTTGACAAGGGAACCTGAACTCCAGAAGTTGTGGGAAGAGAACCAGGTCTTCAAGAGAGTTTCTGATAATAACAATGGAGGGAGTTTCATTCTTCATGATGGTCCTCCTTATGCCAATGGTGATCTGCATATGGGTCATGCTCTTAACAAGATACTGAAGGATATCATTAATCGCTATAAGCTGCTCCAAAACTATAAAGTTCAGTACGTTCCTGGGTGGGATTGCCATGGCCTTCCAATTGAGTTGAAAGTTCTGCAGTCCCTGGATCAGGAAGTAAGAAAGGAACTTACACCATTAAAATTAAGGGCAAAGGCGGCGAAATTTGCAAAAGCAACAGTCAAAACACAAATGGAATCATTTAAGCGATTTGGAGTATGGGCAGACTGGAACAATCCTTATCTAACTCTTGATCCGGAATATGAAGCAGCTCAGATTGAAGTATTTGGCCAGATGGCCTTGAAAGGGTACATTTATAGAGGTAGGAAACCAGTTCACTGGAGCCCTTCATCTCGGACTGCTCTTGCAGAAGCTGAACTAGAGTATCCGGAGGGTCATATTTCCAAAAGCATATATGCTATTTTTAAATTGGTTGGTGGAGCGAAAACAAGCCTTTTAGATGAGTTTATCCCTAATATATACTTGGCAGTATGGACAACTACACCTTGGACTATGCCAGCCAATGCTGCTGTCGCGGTGAATGCAAAGCTTCAGTACTCTGTTGTGGAAGTGCAGTCATTCTCAGAAGATGAATCAACAGTGACAAGCAATAAAAAGAAAATTCCCGGGAAGGTTCTAAAGAATCAACAAAAGCTTTTCGTGATTGTAGCGACCGACCTTGTGCCAGCATTAGAAGCGAAATGGGGTGTGAAGCTTAGCATAAGTAAAACATTCCTTGGTTCAGATCTTGAAAACTGCAGGTACACTCATCCCATTGACAACAGAGACTGTCCAGTCGTTATAGGGGGTGATTACATAACTACAGAATCTGGAACAGGGTTGGTCCACACTGCCCCTGGTCATGGTCAGGAGGATTATGCAACTGGCCTGAAGTACGGGCTGCCTCTTGTCTCTCCGGTGGATGATGAAGGAAAGTTCACTGAAGAAGCTGGACAGTTTAGAGGGCTCTCTGTCCTTGGAGAAGGGAATACCGCTGTTGTCAGTTACCTGGATGAAAATATGTCTCTGGTCATGGAAGAATCTTATGCTCATAAATACCCATATGATTGGCGAACTAAGAAACCGACGATATTTAGAGCGACTGAGCAGTGGTTTGCATCGGTTGAAGGGTTTCGCACAGCCACAATGGATGCAATCAACAATGTAAAATGGGTACCACATCAGGCAGTAAACAGAATATCTGCCATGACTTCCAGTCGATCTGATTGGTGCATCTCAAGACAAAGGACATGGGGTGTCCCTATTCCTGCTTTTTATCATGTTAAGACAAAAGAACCACTCATGAATGAAGAGACAATCAACCATGTTAAATCTATAATTTCCCAAAAGGGTAGTGATGCGTGGTGGTATATGTCAGTGGAAGACCTACTTCCTGAGAAATATCGTGATAAAGCAGCTGATTATGAAAAGGGGACTGATACGATGGACGTCTGGTTTGACTCAGGATCTTCTTGGGCTGGTGTATTAGGGAAACGCGAAGGTCTTAGTTTCCCTGCAGACGTGTATCTAGAAGGTACAGATCAGCATCGTGGATGGTTCCAAAGTTCTTTGTTAACAAGCATCGCAACGCAAGGAAAAGCCCCCTATTCTGCTGTTATAACACATGGATTTGTATTGGATGAGAAGGGTATGAAAATGAGCAAGTCTCTAGGTAACGTAGTGGACCCACGTTTGGTCATTGAAGGAGGGAAAAATTCAAAGGACGCACCTGCTTACGGAGCTGATGTTATGCGTCTTTGGGTTTCTAGTGTAGATTACACAGGGGATGTACTAATAGGTCCCCAGATTCTTCGTCAAATGTCTGATATATATAGGAAGTTAAGGGGAACGTTAAGATACCTCTTGGGAAATCTTCATGATTGGAGAGTTGATAATGCTGTTCCATACCAGGATTTACCTATCATTGATCAGCATGCTCTCTTTCAGCTTGAGAATGTTGTAAAGAATATACAAGAATGTTACGAGAACTACCAGTTCTTTAAAATATTCCAGATCATACAACGATTCACAATTGTTGATCTGTCAAATTTCTACTTCGATATAGCTAAAGATAGACTGTATACTGGGGGTACTTCAAGTTTTACCAGAAGAAGCTGTCAAACAGTTCTTTCAACACATCTTTTATCCATATTGAGAGTGATTGCTCCAATCGTACCTCACCTAGCGGAAGATGTCTGGCAGAATCTTCCATTTGAGTACAGAAATGAAGATGGCTCTGCAGCAGAATTTGTCTTCGAACTTAAATGGCCTACTTTGAATGAACAATGGCTTTCATTTCCTGCTGAAGATGTTCTCTTCTGGCAAAGGCTTCTCGAGTTGAGGACTGAGGTGAACAAAGTGCTGGAGCTAGCACGTAATGAGAAAATGATTGGTTCCAGTTTAGAAGCAAAGGTGTATTTGCATACTGCAGATGCAGGCATGGCTGCAAAATTACTAGAGATGAGTGAAGCAAAGAACGAGGCTGACACTCTGCAACGCATATTCATAACATCACAAGTTGAAGTATTGTCTTCAATGGAGAAGGAGATGATAAGCAGTGTGCAACACACAGGAGAGTATGTGGAGGGAGAGAACAAAGTGTGGATTGGGGTGTCACGAGCCGAGGGGTCTAAGTGTGAGAGGTGCTGGAATTACTCGGGCCAGGTCGGATCATTTTCTGACCATCCTACTCTCTGTGGACGCTGCTTCAGTGTCATTGTTGCTAATCCACCTGAGCCTGCAGTTGCAGCTGTGAACAGCTTAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 zf-FPG_IleRS

Protein Analysis

1093

Amino Acids

123.01

Weight (kDa)

6.68

Isoelectric Point (pI)

38.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	126 - 768	2.8e-136	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	155 - 299	1.5e-12	tRNA synthetases class I (M)
Anticodon_1	PF08264	814 - 977	3.9e-31	Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS	PF06827	1047 - 1076	5.8e-06	Zinc finger found in FPG and IleRS

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49030 AT5G49030 AT5G49030
fragaria_vesca	FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060
malus_domestica	MD13G1168400.v1.1 MD16G1168800.v1.1
prunus_persica	Prupe.1G136500_v2.0.a1 Prupe.1G136500_v2.0.a1
pyrus_communis	pycom13g14400 pycom16g14210
rosa_chinensis	RchiOBHm_Chr4g0422271
rosa_laevigata	RLG00000007623
rosa_multiflora	Rmu_sc0013245.1_g000018
rosa_roxburghii	Rroxscaffold_5G00364380
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238300 Rh4BG241400 Rh4CG253300 Rh4DG237000
rosa_wichuraiana	Rw4G020620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2132
AarI	CACCTGC	1 cut(s)	2251
AasI	GACNNNNNNGTC	1 cut(s)	1307
AatII	GACGTC	1 cut(s)	1965
Acc36I	ACCTGC	2 cut(s)	1263, 2251
Acc65I	GGTACC	1 cut(s)	1687
AccB1I	GGYRCC	1 cut(s)	1687
AccB7I	CCANNNNNTGG	1 cut(s)	332
AccI	GTMKAC	1 cut(s)	2594
AccII	CGCG	3 cut(s)	26, 1049, 2010
AccIII	TCCGGA	2 cut(s)	769, 901
AciI	CCGC	5 cut(s)	26, 671, 1049, 1506, 2699
AclI	AACGTT	1 cut(s)	2363
AclWI	GGATC	4 cut(s)	630, 761, 1987, 3190
AcoI	YGGCCR	1 cut(s)	802
AcsI	RAATTY	7 cut(s)	106, 352, 677, 1134, 2227, 2560, 2759
AcuI	CTGAAG	7 cut(s)	297, 533, 1049, 1424, 1476, 2838, 3205
AcyI	GRCGYC	1 cut(s)	1962
AfiI	CCNNNNNNNGG	7 cut(s)	150, 332, 625, 819, 1376, 2206, 2249
AflIII	ACRYGT	1 cut(s)	2034
AhdI	GACNNNNNGTC	1 cut(s)	1728
AjiI	CACGTC	1 cut(s)	2035
AjnI	CCWGG	8 cut(s)	35, 399, 568, 618, 1369, 1521, 2421, 3174
AloI	GAACNNNNNNTCC	2 cut(s)	360, 392
Alw26I	GTCTC	7 cut(s)	190, 246, 1293, 1429, 1542, 1820, 2184
AlwI	GGATC	4 cut(s)	630, 761, 1987, 3190
AlwNI	CAGNNNCTG	4 cut(s)	619, 887, 1302, 1343
Ama87I	CYCGRG	3 cut(s)	1139, 2846, 3169
Aor13HI	TCCGGA	2 cut(s)	769, 901
AoxI	GGCC	7 cut(s)	248, 586, 802, 810, 1399, 2780, 3172
ApoI	RAATTY	7 cut(s)	106, 352, 677, 1134, 2227, 2560, 2759
ArsI	GACNNNNNNTTYG	4 cut(s)	224, 256, 1952, 1984
AseI	ATTAAT	1 cut(s)	525
Asp700I	GAANNNNTTC	3 cut(s)	154, 1644, 2823
Asp718I	GGTACC	1 cut(s)	1687
AspS9I	GGNCC	6 cut(s)	248, 458, 1357, 2197, 2312, 3172
AsuC2I	CCSGG	2 cut(s)	1140, 1141
AsuHPI	GGTGA	5 cut(s)	488, 1063, 1334, 2684, 2875
AsuII	TTCGAA	1 cut(s)	2769
AsuNHI	GCTAGC	1 cut(s)	2881
AvaI	CYCGRG	3 cut(s)	1139, 2846, 3169
AvaII	GGWCC	4 cut(s)	458, 1357, 2197, 2312
BaeI	ACNNNNGTAYC	2 cut(s)	881, 914
BalI	TGGCCA	1 cut(s)	804
BanI	GGYRCC	1 cut(s)	1687
BanII	GRGCYC	2 cut(s)	860, 1482
BauI	CACGAG	2 cut(s)	243, 3129
BbsI	GAAGAC	5 cut(s)	147, 397, 1902, 2758, 3036
BccI	CCATC	8 cut(s)	52, 290, 449, 802, 1951, 2061, 2738, 3204
BceAI	ACGGC	1 cut(s)	109
BciT130I	CCWGG	8 cut(s)	37, 401, 570, 620, 1371, 1523, 2423, 3176
BciVI	GTATCC	1 cut(s)	909
BclI	TGATCA	1 cut(s)	2440
BcnI	CCSGG	2 cut(s)	1140, 1141
BcoDI	GTCTC	7 cut(s)	190, 246, 1293, 1429, 1542, 1820, 2184
BfaI	CTAG	7 cut(s)	893, 2042, 2183, 2280, 2696, 2882, 2969
BfmI	CTRYAG	7 cut(s)	611, 1270, 1335, 2028, 2751, 2940, 3252
BfuAI	ACCTGC	2 cut(s)	1263, 2251
BfuI	GTATCC	1 cut(s)	909
BglII	AGATCT	1 cut(s)	1258
BlpI	GCTNAGC	2 cut(s)	1229, 3274
BmcAI	AGTACT	1 cut(s)	1070
Bme18I	GGWCC	4 cut(s)	458, 1357, 2197, 2312
BmeRI	GACNNNNNGTC	1 cut(s)	1728
BmeT110I	CYCGRG	3 cut(s)	1139, 2846, 3169
BmgBI	CACGTC	1 cut(s)	2035
BmgT120I	GGNCC	6 cut(s)	248, 458, 1357, 2197, 2312, 3172
BmiI	GGNNCC	7 cut(s)	369, 857, 1689, 2072, 2199, 2314, 2908
BmrI	ACTGGG	1 cut(s)	2607
BmsI	GCATC	8 cut(s)	281, 1640, 1654, 1755, 1861, 2068, 2103, 2935
BmtI	GCTAGC	1 cut(s)	2885
BmuI	ACTGGG	1 cut(s)	2607
BpiI	GAAGAC	5 cut(s)	147, 397, 1902, 2758, 3036
BplI	GAGNNNNNCTC	2 cut(s)	2164, 2196
BpmI	CTGGAG	3 cut(s)	362, 874, 2897
Bpu10I	CCTNAGC	1 cut(s)	3246
Bpu1102I	GCTNAGC	2 cut(s)	1229, 3274
Bpu14I	TTCGAA	1 cut(s)	2769
BpuEI	CTTGAG	2 cut(s)	1735, 2483
BpuMI	CCSGG	2 cut(s)	1140, 1141
BsaAI	YACGTR	1 cut(s)	2888
BsaBI	GATNNNNATC	1 cut(s)	2058
BsaHI	GRCGYC	1 cut(s)	1962
BsaI	GGTCTC	1 cut(s)	246
BsaWI	WCCGGW	3 cut(s)	769, 901, 1429
BsaXI	ACNNNNNCTCC	6 cut(s)	360, 390, 2244, 2274, 3078, 3108
Bsc4I	CCNNNNNNNGG	7 cut(s)	150, 332, 625, 819, 1376, 2206, 2249
Bse3DI	GCAATG	1 cut(s)	57
Bse8I	GATNNNNATC	1 cut(s)	2058
BseAI	TCCGGA	2 cut(s)	769, 901
BseBI	CCWGG	8 cut(s)	37, 401, 570, 620, 1371, 1523, 2423, 3176
BseGI	GGATG	8 cut(s)	1282, 1441, 1531, 1669, 2072, 2158, 2305, 3196
BseJI	GATNNNNATC	1 cut(s)	2058
BseLI	CCNNNNNNNGG	7 cut(s)	150, 332, 625, 819, 1376, 2206, 2249
BseMI	GCAATG	1 cut(s)	57
BseMII	CTCAG	7 cut(s)	800, 1110, 1608, 1899, 1989, 2850, 3237
BsgI	GTGCAG	1 cut(s)	1106
Bsh1236I	CGCG	3 cut(s)	26, 1049, 2010
Bsh1285I	CGRYCG	1 cut(s)	1188
BshFI	GGCC	7 cut(s)	250, 588, 804, 812, 1401, 2782, 3174
BshNI	GGYRCC	1 cut(s)	1687
BsiEI	CGRYCG	1 cut(s)	1188
BsiHKCI	CYCGRG	3 cut(s)	1139, 2846, 3169
BsiSI	CCGG	4 cut(s)	770, 902, 1140, 1430
BslFI	GGGAC	4 cut(s)	601, 1757, 1960, 2298
BslI	CCNNNNNNNGG	7 cut(s)	150, 332, 625, 819, 1376, 2206, 2249
BsmAI	GTCTC	7 cut(s)	190, 246, 1293, 1429, 1542, 1820, 2184
BsmFI	GGGAC	4 cut(s)	601, 1757, 1960, 2298
BsmI	GAATGC	1 cut(s)	1060
BsnI	GGCC	7 cut(s)	250, 588, 804, 812, 1401, 2782, 3174
Bso31I	GGTCTC	1 cut(s)	246
BsoBI	CYCGRG	3 cut(s)	1139, 2846, 3169
Bsp119I	TTCGAA	1 cut(s)	2769
Bsp1286I	GDGCHC	2 cut(s)	860, 1482
Bsp13I	TCCGGA	2 cut(s)	769, 901
Bsp1720I	GCTNAGC	2 cut(s)	1229, 3274
Bsp19I	CCATGG	1 cut(s)	583
BspACI	CCGC	5 cut(s)	26, 671, 1049, 1506, 2699
BspANI	GGCC	7 cut(s)	250, 588, 804, 812, 1401, 2782, 3174
BspCNI	CTCAG	7 cut(s)	799, 1109, 1609, 1900, 1988, 2851, 3238
BspEI	TCCGGA	2 cut(s)	769, 901
BspFNI	CGCG	3 cut(s)	26, 1049, 2010
BspHI	TCATGA	3 cut(s)	451, 1815, 2389
BspLI	GGNNCC	7 cut(s)	369, 857, 1689, 2072, 2199, 2314, 2908
BspMAI	CTGCAG	6 cut(s)	615, 1274, 2032, 2755, 2944, 3256
BspMI	ACCTGC	2 cut(s)	1263, 2251
BspOI	GCTAGC	1 cut(s)	2885
BspPI	GGATC	4 cut(s)	630, 761, 1987, 3190
BspT104I	TTCGAA	1 cut(s)	2769
BspT107I	GGYRCC	1 cut(s)	1687
BspTNI	GGTCTC	1 cut(s)	246
BsrDI	GCAATG	1 cut(s)	57
BssNAI	GTATAC	1 cut(s)	2595
BssNI	GRCGYC	1 cut(s)	1962
BssSI	CACGAG	2 cut(s)	243, 3129
BssT1I	CCWWGG	5 cut(s)	144, 583, 1019, 1249, 1489
Bst1107I	GTATAC	1 cut(s)	2595
Bst2BI	CACGAG	2 cut(s)	243, 3129
Bst2UI	CCWGG	8 cut(s)	37, 401, 570, 620, 1371, 1523, 2423, 3176
Bst4CI	ACNGT	8 cut(s)	192, 316, 694, 1114, 1304, 1470, 2592, 2638
Bst6I	CTCTTC	3 cut(s)	387, 1818, 2834
BstACI	GRCGYC	1 cut(s)	1962
BstBAI	YACGTR	1 cut(s)	2888
BstBI	TTCGAA	1 cut(s)	2769
BstC8I	GCNNGC	6 cut(s)	1032, 1200, 2448, 2883, 2950, 3252
BstDSI	CCRYGG	1 cut(s)	583
BstENI	CCTNNNNNAGG	1 cut(s)	623
BstF5I	GGATG	8 cut(s)	1282, 1441, 1531, 1669, 2072, 2158, 2305, 3196
BstFNI	CGCG	3 cut(s)	26, 1049, 2010
BstMAI	GTCTC	7 cut(s)	190, 246, 1293, 1429, 1542, 1820, 2184
BstMCI	CGRYCG	1 cut(s)	1188
BstNI	CCWGG	8 cut(s)	37, 401, 570, 620, 1371, 1523, 2423, 3176
BstNSI	RCATGY	1 cut(s)	2450
BstSFI	CTRYAG	7 cut(s)	611, 1270, 1335, 2028, 2751, 2940, 3252
BstUI	CGCG	3 cut(s)	26, 1049, 2010
BstV2I	GAAGAC	5 cut(s)	147, 397, 1902, 2758, 3036
BstX2I	RGATCY	2 cut(s)	1258, 1979
BstXI	CCANNNNNNTGG	2 cut(s)	852, 1581
BstYI	RGATCY	2 cut(s)	1258, 1979
BstZ17I	GTATAC	1 cut(s)	2595
BsuI	GTATCC	1 cut(s)	909
BsuRI	GGCC	7 cut(s)	250, 588, 804, 812, 1401, 2782, 3174
BtgI	CCRYGG	1 cut(s)	583
BtgZI	GCGATG	2 cut(s)	71, 2080
BtrI	CACGTC	1 cut(s)	2035
BtsCI	GGATG	8 cut(s)	1282, 1441, 1531, 1669, 2072, 2158, 2305, 3196
BtsI	GCAGTG	3 cut(s)	1362, 1628, 3076
BtsIMutI	CAGTG	8 cut(s)	850, 1119, 1362, 1452, 1628, 1896, 3076, 3229
BveI	ACCTGC	2 cut(s)	1263, 2251
Cac8I	GCNNGC	6 cut(s)	1032, 1200, 2448, 2883, 2950, 3252
CaiI	CAGNNNCTG	4 cut(s)	619, 887, 1302, 1343
CciI	TCATGA	3 cut(s)	451, 1815, 2389
Cfr13I	GGNCC	6 cut(s)	248, 458, 1357, 2197, 2312, 3172
Cfr9I	CCCGGG	1 cut(s)	1139
CseI	GACGC	3 cut(s)	2246, 2255, 3222
CsiI	ACCWGGT	1 cut(s)	399
DraI	TTTAAA	2 cut(s)	940, 2515
DrdI	GACNNNNNNGTC	1 cut(s)	1307
DriI	GACNNNNNGTC	1 cut(s)	1728
DseDI	GACNNNNNNGTC	1 cut(s)	1307
EaeI	YGGCCR	1 cut(s)	802
Eam1104I	CTCTTC	3 cut(s)	387, 1818, 2834
Eam1105I	GACNNNNNGTC	1 cut(s)	1728
EarI	CTCTTC	3 cut(s)	387, 1818, 2834
Eco130I	CCWWGG	5 cut(s)	144, 583, 1019, 1249, 1489
Eco24I	GRGCYC	2 cut(s)	860, 1482
Eco31I	GGTCTC	1 cut(s)	246
Eco32I	GATATC	1 cut(s)	520
Eco47I	GGWCC	4 cut(s)	458, 1357, 2197, 2312
Eco57I	CTGAAG	7 cut(s)	297, 533, 1049, 1424, 1476, 2838, 3205
Eco88I	CYCGRG	3 cut(s)	1139, 2846, 3169
EcoNI	CCTNNNNNAGG	1 cut(s)	623
EcoO109I	RGGNCCY	1 cut(s)	2312
EcoRI	GAATTC	1 cut(s)	352
EcoRII	CCWGG	8 cut(s)	35, 399, 568, 618, 1369, 1521, 2421, 3174
EcoRV	GATATC	1 cut(s)	520
EcoT14I	CCWWGG	5 cut(s)	144, 583, 1019, 1249, 1489
EcoT38I	GRGCYC	2 cut(s)	860, 1482
ErhI	CCWWGG	5 cut(s)	144, 583, 1019, 1249, 1489
FalI	AAGNNNNNCTT	2 cut(s)	1887, 1919
FaqI	GGGAC	4 cut(s)	601, 1757, 1960, 2298
FauI	CCCGC	1 cut(s)	19
FauNDI	CATATG	2 cut(s)	486, 1576
FbaI	TGATCA	1 cut(s)	2440
FblI	GTMKAC	1 cut(s)	2594
FokI	GGATG	8 cut(s)	1269, 1448, 1538, 1676, 2079, 2165, 2312, 3183
FriOI	GRGCYC	2 cut(s)	860, 1482
FspBI	CTAG	7 cut(s)	893, 2042, 2183, 2280, 2696, 2882, 2969
GsuI	CTGGAG	3 cut(s)	362, 874, 2897
HaeIII	GGCC	7 cut(s)	250, 588, 804, 812, 1401, 2782, 3174
HapII	CCGG	4 cut(s)	770, 902, 1140, 1430
HgaI	GACGC	3 cut(s)	2246, 2255, 3222
Hin1I	GRCGYC	1 cut(s)	1962
HincII	GTYRAC	1 cut(s)	2088
HindII	GTYRAC	1 cut(s)	2088
HindIII	AAGCTT	3 cut(s)	1061, 1166, 1226
HpaI	GTTAAC	1 cut(s)	2088
HpaII	CCGG	4 cut(s)	770, 902, 1140, 1430
HphI	GGTGA	5 cut(s)	488, 1063, 1334, 2684, 2875
Hpy99I	CGWCG	1 cut(s)	1603
HpyCH4III	ACNGT	8 cut(s)	192, 316, 694, 1114, 1304, 1470, 2592, 2638
HpyCH4IV	ACGT	7 cut(s)	564, 1962, 2034, 2190, 2203, 2363, 2887
HpySE526I	ACGT	7 cut(s)	564, 1962, 2034, 2190, 2203, 2363, 2887
Hsp92I	GRCGYC	1 cut(s)	1962
Kpn2I	TCCGGA	2 cut(s)	769, 901
KpnI	GGTACC	1 cut(s)	1691
Ksp22I	TGATCA	1 cut(s)	2440
KspAI	GTTAAC	1 cut(s)	2088
LmnI	GCTCC	6 cut(s)	546, 855, 953, 2252, 2683, 2878
LweI	GCATC	8 cut(s)	281, 1640, 1654, 1755, 1861, 2068, 2103, 2935
MabI	ACCWGGT	1 cut(s)	399
MaeI	CTAG	7 cut(s)	893, 2042, 2183, 2280, 2696, 2882, 2969
MaeII	ACGT	7 cut(s)	564, 1962, 2034, 2190, 2203, 2363, 2887
MaeIII	GTNAC	5 cut(s)	1114, 1517, 2186, 2492, 3126
MfeI	CAATTG	2 cut(s)	594, 2544
MflI	RGATCY	2 cut(s)	1258, 1979
MhlI	GDGCHC	2 cut(s)	860, 1482
MlsI	TGGCCA	1 cut(s)	804
MluNI	TGGCCA	1 cut(s)	804
MlyI	GAGTC	1 cut(s)	1967
MmeI	TCCRAC	2 cut(s)	108, 3160
Mox20I	TGGCCA	1 cut(s)	804
MroI	TCCGGA	2 cut(s)	769, 901
MroXI	GAANNNNTTC	3 cut(s)	154, 1644, 2823
MscI	TGGCCA	1 cut(s)	804
Msp20I	TGGCCA	1 cut(s)	804
MspA1I	CMGCKG	3 cut(s)	1508, 1931, 3263
MspI	CCGG	4 cut(s)	770, 902, 1140, 1430
MunI	CAATTG	2 cut(s)	594, 2544
Mva1269I	GAATGC	1 cut(s)	1060
MvaI	CCWGG	8 cut(s)	37, 401, 570, 620, 1371, 1523, 2423, 3176
MvnI	CGCG	3 cut(s)	26, 1049, 2010
NciI	CCSGG	2 cut(s)	1140, 1141
NcoI	CCATGG	1 cut(s)	583
NdeI	CATATG	2 cut(s)	486, 1576
NheI	GCTAGC	1 cut(s)	2881
NlaIV	GGNNCC	7 cut(s)	369, 857, 1689, 2072, 2199, 2314, 2908
NmeAIII	GCCGAG	1 cut(s)	3160
NmuCI	GTSAC	2 cut(s)	1114, 3126
NspI	RCATGY	1 cut(s)	2450
NspV	TTCGAA	1 cut(s)	2769
PaeI	GCATGC	1 cut(s)	2450
PaeR7I	CTCGAG	1 cut(s)	2846
PagI	TCATGA	3 cut(s)	451, 1815, 2389
PaqCI	CACCTGC	1 cut(s)	2251
PasI	CCCWGGG	1 cut(s)	36
PcsI	WCGNNNNNNNCGW	1 cut(s)	241
PctI	GAATGC	1 cut(s)	1060
PdmI	GAANNNNTTC	3 cut(s)	154, 1644, 2823
PfeI	GAWTC	9 cut(s)	131, 710, 1106, 1156, 1340, 1553, 2320, 2538, 2716
PflMI	CCANNNNNTGG	1 cut(s)	332
PleI	GAGTC	1 cut(s)	1967
PpsI	GAGTC	1 cut(s)	1967
Ppu21I	YACGTR	1 cut(s)	2888
PpuMI	RGGWCCY	1 cut(s)	2312
PshBI	ATTAAT	1 cut(s)	525
PsiI	TTATAA	1 cut(s)	2132
Psp1406I	AACGTT	1 cut(s)	2363
Psp5II	RGGWCCY	1 cut(s)	2312
Psp6I	CCWGG	8 cut(s)	35, 399, 568, 618, 1369, 1521, 2421, 3174
PspGI	CCWGG	8 cut(s)	35, 399, 568, 618, 1369, 1521, 2421, 3174
PspN4I	GGNNCC	7 cut(s)	369, 857, 1689, 2072, 2199, 2314, 2908
PspPI	GGNCC	6 cut(s)	248, 458, 1357, 2197, 2312, 3172
PspPPI	RGGWCCY	1 cut(s)	2312
PsrI	GAACNNNNNNTAC	2 cut(s)	85, 117
PstI	CTGCAG	6 cut(s)	615, 1274, 2032, 2755, 2944, 3256
PstNI	CAGNNNCTG	4 cut(s)	619, 887, 1302, 1343
PsuI	RGATCY	2 cut(s)	1258, 1979
PvuII	CAGCTG	2 cut(s)	1931, 3263
Sau96I	GGNCC	6 cut(s)	248, 458, 1357, 2197, 2312, 3172
ScaI	AGTACT	1 cut(s)	1070
SchI	GAGTC	1 cut(s)	1967
SduI	GDGCHC	2 cut(s)	860, 1482
SexAI	ACCWGGT	1 cut(s)	399
SfaNI	GCATC	8 cut(s)	281, 1640, 1654, 1755, 1861, 2068, 2103, 2935
SfcI	CTRYAG	7 cut(s)	611, 1270, 1335, 2028, 2751, 2940, 3252
Sfr274I	CTCGAG	1 cut(s)	2846
SfuI	TTCGAA	1 cut(s)	2769
SinI	GGWCC	4 cut(s)	458, 1357, 2197, 2312
SlaI	CTCGAG	1 cut(s)	2846
SmaI	CCCGGG	1 cut(s)	1141
SmlI	CTYRAG	3 cut(s)	1750, 2462, 2846
SmoI	CTYRAG	3 cut(s)	1750, 2462, 2846
SphI	GCATGC	1 cut(s)	2450
SsiI	CCGC	5 cut(s)	26, 671, 1049, 1506, 2699
SspI	AATATT	1 cut(s)	2520
SspMI	CTAG	7 cut(s)	893, 2042, 2183, 2280, 2696, 2882, 2969
StyI	CCWWGG	5 cut(s)	144, 583, 1019, 1249, 1489
TaaI	ACNGT	8 cut(s)	192, 316, 694, 1114, 1304, 1470, 2592, 2638
TaiI	ACGT	7 cut(s)	567, 1965, 2037, 2193, 2206, 2366, 2890
TaqI	TCGA	4 cut(s)	1732, 2571, 2769, 2847
TaqII	GACCGA	1 cut(s)	1202
TatI	WGTACW	4 cut(s)	303, 1068, 2302, 2730
TauI	GCSGC	1 cut(s)	674
TfiI	GAWTC	9 cut(s)	131, 710, 1106, 1156, 1340, 1553, 2320, 2538, 2716
TscAI	CASTG	8 cut(s)	857, 1119, 1369, 1459, 1628, 1896, 3076, 3229
TseFI	GTSAC	2 cut(s)	1114, 3126
Tsp45I	GTSAC	2 cut(s)	1114, 3126
TspGWI	ACGGA	2 cut(s)	148, 2265
TspMI	CCCGGG	1 cut(s)	1139
TspRI	CASTG	8 cut(s)	857, 1119, 1369, 1459, 1628, 1896, 3076, 3229
Van91I	CCANNNNNTGG	1 cut(s)	332
VpaK11BI	GGWCC	4 cut(s)	458, 1357, 2197, 2312
VspI	ATTAAT	1 cut(s)	525
XagI	CCTNNNNNAGG	1 cut(s)	623
XapI	RAATTY	7 cut(s)	106, 352, 677, 1134, 2227, 2560, 2759
XbaI	TCTAGA	1 cut(s)	2041
XceI	RCATGY	1 cut(s)	2450
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1367
XhoI	CTCGAG	1 cut(s)	2846
XmaI	CCCGGG	1 cut(s)	1139
XmiI	GTMKAC	1 cut(s)	2594
XmnI	GAANNNNTTC	3 cut(s)	154, 1644, 2823
XspI	CTAG	7 cut(s)	893, 2042, 2183, 2280, 2696, 2882, 2969
ZraI	GACGTC	1 cut(s)	1963
ZrmI	AGTACT	1 cut(s)	1070

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.