RchiOBHm_Chr4g0422271

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007310
Physical Location & Seq
Forward (+)
47935537 .. 47948861
13325 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39187

Sequence Viewer

Length: 3267 bp
ATGAATTTCAAATCCTTGGCTTCAAGCTCATCCAGAGAAGCCGCCACAATGGCCATGATTCAAACCACTCCATACAAGGTTCTGTCGCAAAGAACTCGCTCAACTTTTCGGGGCACAGCTTCTGTTGGCTTGTTTTATTTCCGAGGAAGGTCTTCTGCGAAAGAATTCTCCCTCTTTAATATCACACACTATTCTACCCATTCCAGTGATGAGTTTGCTTCTTCCTCAAAGCGAAGGTCTCGGGGACCTGTTATGGCTGCAAAGAAAGCGGCTGAAGGGGGAAAGCAGCAGGATGGAAAATACAAGCACACGGTCGATCTGCCAAAGACATCATTTGGCATGAGAGCGAATTCTTCAACACGAGAGCCTGAACTTCAGAAAATATGGGAGGACAATCAAGTGTTCAAGAGAGTTGTTAGTAAAAATACAGGGGAAAACTTCATTCTTCATGATGGTCCTCCGTACGCCAATGGTGATCTGCACATCGGCCATGCTCTAAATAAGATTTTAAAGGATATGATTAATCGTTATAAGCTTCTTCAGAATTATAAAGTTCATTATGTGCCTGGTTGGGATTGTCATGGCTTACCAATTGAATTGAAAGTCCTGCAATCCCTTGATCAAGCTGCTCGAAAGGATCTCACACCAATAAAGTTGAGAGCGAAGGCAGCTAAGTTTGCCAAGCAAACAGTTAAAAATCAGATGGAATCATTTAAGCGTTATGGAATATGGGCAGACTGGAATAATCCCTACCTGACTCTTGATCCAGAATATGAAGCTGCTCAGATAGAAGTGTTTGGTCAGATGGTCCTTCAAGGTTTTATCTACAGAGGCAGGAAACCAGTTCACTGGAGTCCCTCATCACGAACTGCCCTTGCAGAGGCTGAGTTGGAGTATCCGGAGAGTCATGTTTCAAGGAGCATCTATACCGCTTTCAAATTAGTGAGTGCACCTCCAACTTCAGGTGGCTTATTGAATGAGTATCCAGATGTTTGCTTGGCCATTTGGACAACTACTCCCTGGACTATTCCAGCAAATGCCGCGGTTGCAGTGAATGCCAAGCTTATATATGCCATTGTTGAAGTACAGTCAGTCTCCGAAGATGTTTCTTCATCTGCCGGAAATAGCAAGCAAACGCCTGCTAATTTTCTCAAGGAAGAAAAGAAGCCTTTCCTCATTGTTGCTTCAGATCTTGTGCCAACTCTAGAAGCAAAATGGGGCCTGAAGCTTGTTGTTAGGAAAAGGGTGTCAGGTTCATATCTTGAGAACTGCAGGTATATCCATCCAATATTTAATAGAGAATGCTCAGTTGTGATTGGAGGAGATTATATCACAACAGAGTCGGGAACTGGACTGGTCCATACAGCTCCTGGACATGGTCAGGAAGATTATGTCACTGGCCTTAAGTATGGACTGCCTATTCTTTCTCCAGTAGATGATGATGGAAAGTTCACAGAAGAAGCTGGAAAATTTTGTGGGCTTGATGTACTTGCTGATGGTAATATTGCTGTTGTGAAATACTTGGATGAGCATTTGTCACTGATCATGGAAGAATCCTACAAACATAAGTATCCATATGATTGGAGAACAAAAAAGCCCACTATATTTAGGGCAACTGAGCAATGGTTTGCATCAGTGGAAGGCTTTCGTGAGGCTGTTATGGATGCAATTGGCAATGTAAAATGGATTCCACCTAAGGCAGAAAACAGAATATCTGCAATGACTTCTAGCCGCTCTGATTGGTGTATATCGCGGCAGAGGACTTGGGGTGTTCCAATTCCAGTCTTTTATCACCTCCAGTCAAAGGAACCTCTAATGAATGAGGAGACTATTGATCATATCAAGTCTATAATTTCTGAAAAGGGTAGCGATGCATGGTGGTACATGAAGGTAGAGGATCTTCTCCCTAATAAATATCGTGATAAAGCATCCGAATATGAAAAGGGAACTGACACAATGGATGTATGGTTCGACTCAGGCTCTTCTTGGGCTGCAGTTTTAGGAAAAAGAAAAAGCCTTAGTCTCCCTGCAGACTTGTACCTTGAAGGTATGGATCAGCATCGTGGGTGGTTCCAGAGTTCTTTGTTAACAAGTGTTGCTACAAAAGGAAAGGCTCCATATTCCAGTGTCATAACACATGGATTTGTATTGGATGAGAAGGGTTTAAAGATGAGCAAATCTCAGGGTAACGTTGTAGACCCAAGAACTGTGATTGAAGGAGGGAAGAACCAAAAGGATGGCTATGGAGCTGATGTTCTGCGTCTCTGGGTTTCCAGCATAGATTATACAGGCGATGTTATGATAGGTGCTCAAGTTCTCCGTCAAATGTCAGATATATATAGGAAGCTACGAGGAACATTGAGATACCTTTTGGGAAATCTGCATGATTGGCATGCTGATAATGCTATTTCATACCACGATCTTCCCATGATTGATCAGCATGCACTGTTTCAACTTGAAAATTTTGTAAACAACAGCAGAGAGTGCTATGAAAGTTATCAGTTCTTTAAGATATTTCAGATCATACAGCGGTTTGTCATTGTTGACCTATCAAATTTCTACTTTGATATTGCCAAAGATCGTTTATACGTTGGGGGCACGACAAGTTTTACTAGGAGAAGTTGTCAGACGGTTCTTGCAGAACTGCTCCTTTCTATTGTGAGAGTGATTGCTCCAATATTGCCTCATTTGGCTGAGGATGTATGGCAAAATCTTCCATTCCAGTACACTGACAAAAATGGCTCTGTTGCGGAATTTGTTTTTGAATCAAGATGGCCAGCTTCGAACGAAACATGGCTCTCTCTTTCTAAAGAGGAAACTGATTTCTGGACTAAAATTCTTGAGCTGAGAACCGAGGTGAATAAAGTGTTAGAGATTGCTCGTACAACAAAGTTAATTGGCTCTAGCTTAGATGCCAAGGTCTACCTCCATACATCGGATTCTGGTCTGGCCTCAAGATTGGTTCAAATATCTGCAGCCAAGAACGATGCAGACACATTGAATCGGATATTCATAACTTCCCAGGCAGAGGTTCTTCCATCCTTAGAAGATGACTGGATTGCGAATATACCACATAAAGGAGAATGTCAAGTTGACGAAAGCATTAAAGTTTGGATTGGAGTGTCTCGCGCTGAGGGTCTAAAGTGTGAAAGATGCTGGAACTATTCACCTCAGGTTGGTTCCTTTCCAGACCACAGCACTCTTTGCAGCCGCTGCTATGATGTGGTTGATATTCAACAAGCTGCAGCTGAAGCTGTTGTCGTTTGA

Protein Analysis

1088

Amino Acids

122.73

Weight (kDa)

7.35

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 125 - 767 8.8e-134 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 154 - 298 5.4e-13 tRNA synthetases class I (M)
Anticodon_1 PF08264 812 - 975 5.1e-30 Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS PF06827 1046 - 1075 4.9e-08 Zinc finger found in FPG and IleRS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 531, 549
Acc36I ACCTGC 1 cut(s) 1263
AccBSI CCGCTC 1 cut(s) 1734
AccI GTMKAC 2 cut(s) 2196, 2922
AccII CGCG 3 cut(s) 1043, 1753, 3129
AccIII TCCGGA 1 cut(s) 898
AclI AACGTT 1 cut(s) 2190
AclWI GGATC 4 cut(s) 645, 758, 1905, 2061
AcoI YGGCCR 4 cut(s) 51, 487, 999, 2774
AcsI RAATTY 8 cut(s) 4, 164, 349, 1469, 2461, 2554, 2753, 2835
AcuI CTGAAG 6 cut(s) 294, 359, 524, 945, 1170, 1244
AfaI GTAC 7 cut(s) 464, 1086, 1488, 1883, 2039, 2726, 2884
AfiI CCNNNNNNNGG 8 cut(s) 880, 962, 1376, 1804, 2935, 3028, 3077, 3176
AflII CTTAAG 1 cut(s) 1403
AjnI CCWGG 4 cut(s) 565, 1019, 1369, 3021
AloI GAACNNNNNNTCC 4 cut(s) 1952, 1984, 2238, 2270
Alw21I GWGCWC 2 cut(s) 952, 2311
Alw26I GTCTC 6 cut(s) 243, 1099, 1820, 2027, 2267, 3129
Alw44I GTGCAC 1 cut(s) 948
AlwI GGATC 4 cut(s) 645, 758, 1905, 2061
AlwNI CAGNNNCTG 4 cut(s) 122, 884, 1370, 3213
Ama87I CYCGRG 1 cut(s) 240
Aor13HI TCCGGA 1 cut(s) 898
AoxI GGCC 7 cut(s) 51, 487, 999, 1219, 1399, 2774, 2949
ApaLI GTGCAC 1 cut(s) 948
ApoI RAATTY 8 cut(s) 4, 164, 349, 1469, 2461, 2554, 2753, 2835
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
AseI ATTAAT 1 cut(s) 522
Asp700I GAANNNNTTC 4 cut(s) 151, 164, 1169, 1644
AspLEI GCGC 1 cut(s) 3131
AspS9I GGNCC 5 cut(s) 245, 455, 808, 1219, 1357
AsuHPI GGTGA 4 cut(s) 485, 1784, 2869, 3159
AsuII TTCGAA 1 cut(s) 2783
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 4 cut(s) 245, 455, 808, 1357
AxyI CCTNAGG 2 cut(s) 1695, 3171
BaeGI GKGCMC 3 cut(s) 116, 952, 2600
BaeI ACNNNNGTAYC 2 cut(s) 1553, 1586
BalI TGGCCA 3 cut(s) 53, 1001, 2776
BarI GAAGNNNNNNTAC 2 cut(s) 1884, 1916
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 2 cut(s) 952, 2311
BbvCI CCTCAGC 2 cut(s) 2694, 3132
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 4 cut(s) 567, 1021, 1371, 3023
BciVI GTATCC 3 cut(s) 906, 993, 1581
BclI TGATCA 4 cut(s) 619, 1542, 1834, 2434
BcoDI GTCTC 6 cut(s) 243, 1099, 1820, 2027, 2267, 3129
BfaI CTAG 4 cut(s) 1205, 1728, 2613, 2904
BfmI CTRYAG 6 cut(s) 826, 1270, 1992, 2028, 2973, 3243
BfrI CTTAAG 1 cut(s) 1403
BfuAI ACCTGC 1 cut(s) 1263
BfuI GTATCC 3 cut(s) 906, 993, 1581
BglI GCCNNNNNGGC 1 cut(s) 50
BglII AGATCT 1 cut(s) 1189
Bme1390I CCNGG 4 cut(s) 567, 1021, 1371, 3023
Bme18I GGWCC 4 cut(s) 245, 455, 808, 1357
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 5 cut(s) 245, 455, 808, 1219, 1357
BmiI GGNNCC 6 cut(s) 246, 1220, 1809, 2072, 2115, 3181
BmrFI CCNGG 4 cut(s) 567, 1021, 1371, 3023
BmsI GCATC 9 cut(s) 930, 1640, 1654, 1861, 1937, 2068, 2902, 2977, 3143
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 4 cut(s) 937, 969, 2164, 2196
BpmI CTGGAG 3 cut(s) 871, 1413, 1781
Bpu10I CCTNAGC 2 cut(s) 2694, 3132
Bpu14I TTCGAA 1 cut(s) 2783
BpuEI CTTGAG 5 cut(s) 1136, 1283, 2295, 2861, 2938
BsaBI GATNNNNATC 1 cut(s) 2058
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 7 cut(s) 15, 142, 1019, 1041, 2853, 2916, 3021
BsaWI WCCGGW 1 cut(s) 898
BsaXI ACNNNNNCTCC 6 cut(s) 1000, 1030, 2238, 2268, 2608, 2638
Bsc4I CCNNNNNNNGG 8 cut(s) 880, 962, 1376, 1804, 2935, 3028, 3077, 3176
Bse21I CCTNAGG 2 cut(s) 1695, 3171
Bse3DI GCAATG 3 cut(s) 1628, 1681, 1725
Bse8I GATNNNNATC 1 cut(s) 2058
BseAI TCCGGA 1 cut(s) 898
BseBI CCWGG 4 cut(s) 567, 1021, 1371, 3023
BseDI CCNNGG 7 cut(s) 15, 142, 1019, 1041, 2853, 2916, 3021
BseJI GATNNNNATC 1 cut(s) 2058
BseLI CCNNNNNNNGG 8 cut(s) 880, 962, 1376, 1804, 2935, 3028, 3077, 3176
BseMI GCAATG 3 cut(s) 1628, 1681, 1725
BseRI GAGGAG 2 cut(s) 1335, 1838
BseSI GKGCMC 3 cut(s) 116, 952, 2600
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 3 cut(s) 1043, 1753, 3129
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 7 cut(s) 53, 489, 1001, 1221, 1401, 2776, 2951
BsiEI CGRYCG 1 cut(s) 315
BsiHKAI GWGCWC 2 cut(s) 952, 2311
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 2 cut(s) 899, 1119
BsiWI CGTACG 1 cut(s) 462
BslFI GGGAC 2 cut(s) 258, 840
BslI CCNNNNNNNGG 8 cut(s) 880, 962, 1376, 1804, 2935, 3028, 3077, 3176
BsmAI GTCTC 6 cut(s) 243, 1099, 1820, 2027, 2267, 3129
BsmBI CGTCTC 1 cut(s) 2267
BsmFI GGGAC 2 cut(s) 258, 840
BsmI GAATGC 2 cut(s) 1060, 1307
BsnI GGCC 7 cut(s) 53, 489, 1001, 1221, 1401, 2776, 2951
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 240
Bsp119I TTCGAA 1 cut(s) 2783
Bsp1286I GDGCHC 4 cut(s) 116, 952, 2311, 2600
Bsp13I TCCGGA 1 cut(s) 898
BspANI GGCC 7 cut(s) 53, 489, 1001, 1221, 1401, 2776, 2951
BspEI TCCGGA 1 cut(s) 898
BspFNI CGCG 3 cut(s) 1043, 1753, 3129
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 6 cut(s) 246, 1220, 1809, 2072, 2115, 3181
BspMAI CTGCAG 5 cut(s) 1274, 1996, 2032, 2977, 3247
BspMI ACCTGC 1 cut(s) 1263
BspPI GGATC 4 cut(s) 645, 758, 1905, 2061
BspQI GCTCTTC 1 cut(s) 1987
BspT104I TTCGAA 1 cut(s) 2783
BspTI CTTAAG 1 cut(s) 1403
BspTNI GGTCTC 1 cut(s) 243
BsrBI CCGCTC 1 cut(s) 1734
BsrDI GCAATG 3 cut(s) 1628, 1681, 1725
BssECI CCNNGG 7 cut(s) 15, 142, 1019, 1041, 2853, 2916, 3021
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 15, 2916
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 4 cut(s) 567, 1021, 1371, 3023
Bst4CI ACNGT 6 cut(s) 313, 691, 1089, 2209, 2448, 2632
Bst6I CTCTTC 1 cut(s) 1987
BstAFI CTTAAG 1 cut(s) 1403
BstAPI GCANNNNNTGC 4 cut(s) 1055, 2484, 3204, 3213
BstBI TTCGAA 1 cut(s) 2783
BstC8I GCNNGC 5 cut(s) 1130, 1140, 2394, 2442, 2778
BstDSI CCRYGG 1 cut(s) 1041
BstENI CCTNNNNNAGG 1 cut(s) 878
BstFNI CGCG 3 cut(s) 1043, 1753, 3129
BstHHI GCGC 1 cut(s) 3131
BstMAI GTCTC 6 cut(s) 243, 1099, 1820, 2027, 2267, 3129
BstMCI CGRYCG 1 cut(s) 315
BstNI CCWGG 4 cut(s) 567, 1021, 1371, 3023
BstNSI RCATGY 2 cut(s) 2396, 2444
BstSCI CCNGG 4 cut(s) 565, 1019, 1369, 3021
BstSFI CTRYAG 6 cut(s) 826, 1270, 1992, 2028, 2973, 3243
BstSLI GKGCMC 3 cut(s) 116, 952, 2600
BstUI CGCG 3 cut(s) 1043, 1753, 3129
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 3 cut(s) 637, 1189, 1897
BstXI CCANNNNNNTGG 3 cut(s) 849, 1581, 2237
BstYI RGATCY 3 cut(s) 637, 1189, 1897
Bsu36I CCTNAGG 2 cut(s) 1695, 3171
BsuI GTATCC 3 cut(s) 906, 993, 1581
BsuRI GGCC 7 cut(s) 53, 489, 1001, 1221, 1401, 2776, 2951
BtgI CCRYGG 1 cut(s) 1041
BtgZI GCGATG 2 cut(s) 1884, 2307
BtsI GCAGTG 1 cut(s) 1056
BtsIMutI CAGTG 9 cut(s) 211, 847, 1056, 1395, 1538, 1641, 2131, 2444, 2727
BveI ACCTGC 1 cut(s) 1263
Cac8I GCNNGC 5 cut(s) 1130, 1140, 2394, 2442, 2778
CaiI CAGNNNCTG 4 cut(s) 122, 884, 1370, 3213
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 3131
Cfr13I GGNCC 5 cut(s) 245, 455, 808, 1219, 1357
Cfr42I CCGCGG 1 cut(s) 1044
CseI GACGC 1 cut(s) 2249
Csp6I GTAC 7 cut(s) 463, 1085, 1487, 1882, 2038, 2725, 2883
CviQI GTAC 7 cut(s) 463, 1085, 1487, 1882, 2038, 2725, 2883
DraI TTTAAA 2 cut(s) 510, 2166
EaeI YGGCCR 4 cut(s) 51, 487, 999, 2774
Eam1104I CTCTTC 1 cut(s) 1987
EarI CTCTTC 1 cut(s) 1987
Eco130I CCWWGG 2 cut(s) 15, 2916
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 4 cut(s) 245, 455, 808, 1357
Eco57I CTGAAG 6 cut(s) 294, 359, 524, 945, 1170, 1244
Eco81I CCTNAGG 2 cut(s) 1695, 3171
Eco88I CYCGRG 1 cut(s) 240
EcoNI CCTNNNNNAGG 1 cut(s) 878
EcoO109I RGGNCCY 2 cut(s) 245, 1219
EcoRI GAATTC 2 cut(s) 164, 349
EcoRII CCWGG 4 cut(s) 565, 1019, 1369, 3021
EcoT14I CCWWGG 2 cut(s) 15, 2916
EcoT22I ATGCAT 1 cut(s) 1876
ErhI CCWWGG 2 cut(s) 15, 2916
Esp3I CGTCTC 1 cut(s) 2267
FaqI GGGAC 2 cut(s) 258, 840
FauNDI CATATG 1 cut(s) 1576
FbaI TGATCA 4 cut(s) 619, 1542, 1834, 2434
FblI GTMKAC 2 cut(s) 2196, 2922
FspBI CTAG 4 cut(s) 1205, 1728, 2613, 2904
GlaI GCGC 1 cut(s) 3130
GsuI CTGGAG 3 cut(s) 871, 1413, 1781
HaeIII GGCC 7 cut(s) 53, 489, 1001, 1221, 1401, 2776, 2951
HapII CCGG 2 cut(s) 899, 1119
HgaI GACGC 1 cut(s) 2249
HhaI GCGC 1 cut(s) 3131
Hin6I GCGC 1 cut(s) 3129
HinP1I GCGC 1 cut(s) 3129
HincII GTYRAC 3 cut(s) 2088, 2545, 3094
HindII GTYRAC 3 cut(s) 2088, 2545, 3094
HindIII AAGCTT 3 cut(s) 533, 1061, 1226
HpaI GTTAAC 1 cut(s) 2088
HpaII CCGG 2 cut(s) 899, 1119
HphI GGTGA 4 cut(s) 485, 1784, 2869, 3159
HpyCH4III ACNGT 6 cut(s) 313, 691, 1089, 2209, 2448, 2632
HpyCH4IV ACGT 2 cut(s) 2190, 2589
HpySE526I ACGT 2 cut(s) 2190, 2589
HspAI GCGC 1 cut(s) 3129
Kpn2I TCCGGA 1 cut(s) 898
Ksp22I TGATCA 4 cut(s) 619, 1542, 1834, 2434
KspAI GTTAAC 1 cut(s) 2088
KspI CCGCGG 1 cut(s) 1044
LguI GCTCTTC 1 cut(s) 1987
LmnI GCTCC 6 cut(s) 918, 1372, 2119, 2246, 2652, 2677
LweI GCATC 9 cut(s) 930, 1640, 1654, 1861, 1937, 2068, 2902, 2977, 3143
MaeI CTAG 4 cut(s) 1205, 1728, 2613, 2904
MaeII ACGT 2 cut(s) 2190, 2589
MaeIII GTNAC 3 cut(s) 1393, 1536, 2186
MbiI CCGCTC 1 cut(s) 1734
MfeI CAATTG 2 cut(s) 591, 1668
MflI RGATCY 3 cut(s) 637, 1189, 1897
MhlI GDGCHC 4 cut(s) 116, 952, 2311, 2600
MlsI TGGCCA 3 cut(s) 53, 1001, 2776
MluNI TGGCCA 3 cut(s) 53, 1001, 2776
MlyI GAGTC 5 cut(s) 751, 862, 913, 1349, 1967
MmeI TCCRAC 2 cut(s) 870, 980
Mox20I TGGCCA 3 cut(s) 53, 1001, 2776
Mph1103I ATGCAT 1 cut(s) 1876
MroI TCCGGA 1 cut(s) 898
MroXI GAANNNNTTC 4 cut(s) 151, 164, 1169, 1644
MscI TGGCCA 3 cut(s) 53, 1001, 2776
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 3 cut(s) 53, 1001, 2776
MspA1I CMGCKG 4 cut(s) 1043, 2530, 3213, 3248
MspCI CTTAAG 1 cut(s) 1403
MspI CCGG 2 cut(s) 899, 1119
MspR9I CCNGG 4 cut(s) 567, 1021, 1371, 3023
MunI CAATTG 2 cut(s) 591, 1668
Mva1269I GAATGC 2 cut(s) 1060, 1307
MvaI CCWGG 4 cut(s) 567, 1021, 1371, 3023
MvnI CGCG 3 cut(s) 1043, 1753, 3129
NdeI CATATG 1 cut(s) 1576
NlaIV GGNNCC 6 cut(s) 246, 1220, 1809, 2072, 2115, 3181
NmuCI GTSAC 2 cut(s) 1393, 1536
NsiI ATGCAT 1 cut(s) 1876
NspI RCATGY 2 cut(s) 2396, 2444
NspV TTCGAA 1 cut(s) 2783
PaeI GCATGC 2 cut(s) 2396, 2444
PagI TCATGA 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 1987
PctI GAATGC 2 cut(s) 1060, 1307
PdmI GAANNNNTTC 4 cut(s) 151, 164, 1169, 1644
PfeI GAWTC 7 cut(s) 58, 707, 1553, 1687, 2765, 2939, 3001
Pfl23II CGTACG 1 cut(s) 462
PflFI GACNNNGTC 1 cut(s) 1377
PfoI TCCNGGA 1 cut(s) 1369
PleI GAGTC 5 cut(s) 751, 861, 912, 1348, 1967
PpsI GAGTC 5 cut(s) 751, 861, 912, 1348, 1967
PpuMI RGGWCCY 1 cut(s) 245
PshBI ATTAAT 1 cut(s) 522
PsiI TTATAA 2 cut(s) 531, 549
Psp1406I AACGTT 1 cut(s) 2190
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 4 cut(s) 565, 1019, 1369, 3021
PspGI CCWGG 4 cut(s) 565, 1019, 1369, 3021
PspLI CGTACG 1 cut(s) 462
PspN4I GGNNCC 6 cut(s) 246, 1220, 1809, 2072, 2115, 3181
PspPI GGNCC 5 cut(s) 245, 455, 808, 1219, 1357
PspPPI RGGWCCY 1 cut(s) 245
PstI CTGCAG 5 cut(s) 1274, 1996, 2032, 2977, 3247
PstNI CAGNNNCTG 4 cut(s) 122, 884, 1370, 3213
PsuI RGATCY 3 cut(s) 637, 1189, 1897
PsyI GACNNNGTC 1 cut(s) 1377
PvuII CAGCTG 1 cut(s) 3248
RsaI GTAC 7 cut(s) 464, 1086, 1488, 1883, 2039, 2726, 2884
RsaNI GTAC 7 cut(s) 463, 1085, 1487, 1882, 2038, 2725, 2883
RseI CAYNNNNRTG 1 cut(s) 204
SacII CCGCGG 1 cut(s) 1044
SapI GCTCTTC 1 cut(s) 1987
Sau96I GGNCC 5 cut(s) 245, 455, 808, 1219, 1357
SchI GAGTC 5 cut(s) 751, 862, 913, 1349, 1967
ScrFI CCNGG 4 cut(s) 567, 1021, 1371, 3023
SduI GDGCHC 4 cut(s) 116, 952, 2311, 2600
SfaNI GCATC 9 cut(s) 930, 1640, 1654, 1861, 1937, 2068, 2902, 2977, 3143
SfcI CTRYAG 6 cut(s) 826, 1270, 1992, 2028, 2973, 3243
Sfr303I CCGCGG 1 cut(s) 1044
SfuI TTCGAA 1 cut(s) 2783
SgrBI CCGCGG 1 cut(s) 1044
SinI GGWCC 4 cut(s) 245, 455, 808, 1357
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 6 cut(s) 1151, 1262, 1403, 2310, 2840, 2953
SmoI CTYRAG 6 cut(s) 1151, 1262, 1403, 2310, 2840, 2953
SphI GCATGC 2 cut(s) 2396, 2444
SspI AATATT 3 cut(s) 1290, 1504, 2679
SspMI CTAG 4 cut(s) 1205, 1728, 2613, 2904
StyD4I CCNGG 4 cut(s) 565, 1019, 1369, 3021
StyI CCWWGG 2 cut(s) 15, 2916
TaaI ACNGT 6 cut(s) 313, 691, 1089, 2209, 2448, 2632
TaiI ACGT 2 cut(s) 2193, 2592
TaqI TCGA 4 cut(s) 315, 631, 1971, 2783
TatI WGTACW 3 cut(s) 1084, 1486, 2724
TauI GCSGC 6 cut(s) 44, 272, 1043, 1734, 1756, 3213
TfiI GAWTC 7 cut(s) 58, 707, 1553, 1687, 2765, 2939, 3001
TscAI CASTG 9 cut(s) 211, 854, 1056, 1402, 1545, 1641, 2131, 2451, 2734
TseFI GTSAC 2 cut(s) 1393, 1536
Tsp45I GTSAC 2 cut(s) 1393, 1536
TspGWI ACGGA 2 cut(s) 450, 2309
TspRI CASTG 9 cut(s) 211, 854, 1056, 1402, 1545, 1641, 2131, 2451, 2734
Tth111I GACNNNGTC 1 cut(s) 1377
Vha464I CTTAAG 1 cut(s) 1403
VneI GTGCAC 1 cut(s) 948
VpaK11BI GGWCC 4 cut(s) 245, 455, 808, 1357
VspI ATTAAT 1 cut(s) 522
XagI CCTNNNNNAGG 1 cut(s) 878
XapI RAATTY 8 cut(s) 4, 164, 349, 1469, 2461, 2554, 2753, 2835
XbaI TCTAGA 1 cut(s) 1204
XceI RCATGY 2 cut(s) 2396, 2444
XcmI CCANNNNNNNNNTGG 1 cut(s) 1367
XmiI GTMKAC 2 cut(s) 2196, 2922
XmnI GAANNNNTTC 4 cut(s) 151, 164, 1169, 1644
XspI CTAG 4 cut(s) 1205, 1728, 2613, 2904
Zsp2I ATGCAT 1 cut(s) 1876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.