Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007310

Physical Location & Seq

Genomic Coordinates Forward (+)

13618342 .. 13630033

Gene Structure 11692 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1168400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3282 bp

ATGAACTTCAAATCCTTCGCCGCCCACTCTTTGGCGCTCCAACCCAGGGAGGCCGCCATGGCTGCGATTCAAACCTCCCCCTACCGGGTTCTGTCGCCAAGAACTTGCTCATCCTTTAGGAGCACAGCTTCTGTTGGCTTATTTTATTTCAGAGATAGGTCTTCAGTTAAAGTATTCTCCCTCTTTAACATCGCACACTATTCTACGTATTCCAATGACGAGTTTGGTTCGTCCTCAAAGCGAAGATCTCGAGGACCTGTTATGGCCGCAAAGAAAGTAGCTGAAGGGAGAAAGCAAGAAGAGGGAAAATACAAGCACACAGTTGAGCTGCCAAAGACATCATTTAGCATGAGAGCGAACTCTTTAATGCGAGAGCCTGAAATCCAAAAAATATGGGATGACAATCAAGTGTTCAAAAGAGTTATTGGTAAAAATACAGGGGAAAATTTCATTCTTCACGATGGTCCTCCATATGCCAATGGAGATCTTCACATAGGCCATGCTTTAAATAAGATTTTAAAGGATTTTATCAATCGTTACAAGCTTCTTCAAAATTATAAGGTTCATTATGTGCCTGGTTGGGATTGTCATGGCTTACCAATTGAGTTGAAAGTTCTGCAATCACTTGATCAAGCTGCTCGAAAGGATCTTACACCAATAAAGTTGAGAGCAAAGGCAGCTAAATTTGCCAAGCAAACTGTTAAAACTCAGATGGAATCATTTAAGCGTTATGGAGTGTGGGCAGACTGGAATAATCCTTACCTAACTCTTGATCCAGAATATGAAGCTGCTCAGATAGAAGTATTTGGCCAGATGGTCCTTCAAGGTTTCATCTACAGAGGCAGGAAACCAGTTCACTGGAGCCCCTCATCACGAACTGCTCTTGCAGAGGCTGAGTTGGAGTATCCAGAGGGGCATGTTTCGAGAAGCATATATGCCATTTTCAAATTGGTCAGTGCCTCTCCAACTTCAGGTGGCTTATTAAATGAGTATTTCCCAGATGTGTGCTTGGCCATTTGGACAACAACTCCCTGGACTATTCCAGCCAATGCTGCGGTGGCTGTGAATGCCAAGCTTTTATATGCCATCGTTGAAGTACAGTCACTCCCAGAAGATTCTTTATCTGATGGAAATAAAAAGGGAAGGTCTGGAAATCTTTTGAAGGAAGAAAAGAAGCCTTTCCTTATTGTTGCTTCAGATCTTGTGCCAGCGTTAGAATCAAAATGGGGTGTGAAGCTTGTTGTTAGGAAAAGGGTGTCGGGTTCAGATCTTGAAAACTGCAGGTATGTCCATCCGGTTTTTAATAGGGAATGCCCCGTTGTCATTGGAGGAGATTATATTACAACAGAATCAGGAACAGGACTGGTCCATACAGCTCCTGGACATGGCCAGGAGGATTATGTCACTGGCCAGAAGTACGGACTACCTATGCTTTCTCCTGTAGATGATGATGGAAGGTTCACTGAAGAAGCTGGAAAGTTTTGTGGGCTTGATGTACTTGCTGATGGTAATATTGCTGTCATGAAATACTTGGATGAACATTTATCAATTATCATGGAAGAATCCTACCAGCATAAGTATCCATATGACTGGAGAACAAAAAAACCCACAATATTTAGGGCAACTGAGCAATGGTTTGCTTCGGTAGAAGGGTTTCGTGAGGCTGTTATGGATGCAATTGGTCATGTAAAATGGATTCCACCTAAGGCAGAAAACAGAATTTCTGCAATGACTTCCAGCCGCTCTGATTGGTGTATATCGCGGCAAAGGACATGGGGTGTTCCAATTCCAGTCTTTTATCATGTGGAGTCAAGGGAACCTCTGATGAATGAAGAGACTATTGAGCATATCAAGTCGATAATATCTGAAAAGGGTAGTGATGCGTGGTGGTACATGAAGGTCGAGGATCTTCTCCCTGATAAATATCGTGATAAAGCATCTGATTATGAAAAGGGGACTGATACAATGGATGTATGGTTTGATTCAGGCTCTTCTTGGGCTGCAGTGTTGGGGAAAAGAAATAGCCATAGTCTTCCTGCAGATTTGTACCTTGAGGGTATGGATCAGCATCGTGGGTGGTTCCAAAGTTCTTTGCTAACAAGTGTTGCTACTAAAGGAAAGGCTCCATATTCCAGTGTTATAACACATGGATTTGTATTGGATGAGAAAGGTTTGAAAATGAGCAAATCTTTGGGTAATGTAGTAGATCCACGGACTGTGATTGAAGGAGGGAAGAACCAAAAGGATGGCTATGGAGCGGATGTCCTGCGTCTTTGGGTTTCTAGTGTAGATTATACAGGCGATGTTACAATCGGCCCTCAAATTCTCCGTCAAATGTCAGATATTTATAGGAAGACGCGAGGAACATTGAGATACCTTTTGGGAAATCTTCATGATTGGAATGCTGATAGTGCTATCTCTTACCACGATCTTCCCATGATTGATCAGTACGCACTGTTTCAGCTTGAAATTTTTGTGAAAAACAGTAAAGAGTGCTACGAAAACTATCAGTTCTTTAAGATATTTCAGATCATACAGCGGTTTGTTGTCGTTGACCTGTCAAATTTCTATTTTGATGTCGCCAAAGATCGGCTTTATGTTGGGGGTACAAGTAGTTTTACTAGAAGAAGTTGTCAAACAGTTCTTGCAGCACTTCTCCTCTCTGTAGTGAGAGTGATTGCTCCGATATTACCTCATTTGGCTGAAGATGTATGGCAAAACCTTCCGTTCCAGTACACTGACGATGATGGCTCTGCTGCCAAATTTGTTTTTGAATCAAGATGGCCAGCTTTGAACAACACATGGCTCTCTCTTTCGAAAGAGGAAACTAATTTCTGGGAAAAGGTTCTTGAGTTGAGAACTGAGGTGAACAAAGTCCTGGAGGTTGCTCGTACGGAAAAGCTAATTGGCTCTAGTTTAGATGCCAAGGTCTACCTTCACACTTCGGATTCCGGTCTGGCCTCTAGATTAGGTGAAATGTCTGAGGCCAACAACGACGCAGACACACTGCATCGCATATTCATAACATCTCAGGCAGAGGTTCTTCCTTCGTTGGAAGATGAGCTGATTGCGAACATACCTCACAAAGGAGAATACCTCATCGAAGAAAACGTCAAAGTCTGGATCGGAGTGTCTCGTGCCGAGGGCTTAAAGTGCGAAAGATGCTGGAATTATTCACCTCAAGTTGGTTCCTTTCCAGAGCACAGCACGCTTTGCGGCCGCTGCTATAATGTGGTAAATGTTCAACAATCTCCATCTCCAGCTGTAGCTGTGGTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 zf-FPG_IleRS

Protein Analysis

1094

Amino Acids

123.47

Weight (kDa)

6.75

Isoelectric Point (pI)

43.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	128 - 771	2.5e-133	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	157 - 301	2e-12	tRNA synthetases class I (M)
Anticodon_1	PF08264	815 - 978	7.5e-30	Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS	PF06827	1049 - 1078	3.4e-08	Zinc finger found in FPG and IleRS

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49030 AT5G49030 AT5G49030
fragaria_vesca	FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060
malus_domestica	MD13G1168400.v1.1 MD16G1168800.v1.1
prunus_persica	Prupe.1G136500_v2.0.a1 Prupe.1G136500_v2.0.a1
pyrus_communis	pycom13g14400 pycom16g14210
rosa_chinensis	RchiOBHm_Chr4g0422271
rosa_laevigata	RLG00000007623
rosa_multiflora	Rmu_sc0013245.1_g000018
rosa_roxburghii	Rroxscaffold_5G00364380
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238300 Rh4BG241400 Rh4CG253300 Rh4DG237000
rosa_wichuraiana	Rw4G020620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	558, 2141
Acc36I	ACCTGC	1 cut(s)	1272
AccB7I	CCANNNNNTGG	1 cut(s)	31
AccBSI	CCGCTC	2 cut(s)	1743, 2258
AccI	GTMKAC	1 cut(s)	2931
AccII	CGCG	2 cut(s)	1762, 2359
AclWI	GGATC	6 cut(s)	654, 767, 1914, 2070, 2201, 3131
AcoI	YGGCCR	7 cut(s)	264, 808, 1011, 1387, 1408, 2783, 3217
AcsI	RAATTY	7 cut(s)	445, 683, 1719, 2322, 2469, 2563, 2762
AcuI	CTGAAG	6 cut(s)	147, 303, 954, 1179, 1485, 2724
AfaI	GTAC	9 cut(s)	1098, 1418, 1497, 1892, 2048, 2450, 2608, 2735, 2893
AfiI	CCNNNNNNNGG	9 cut(s)	31, 46, 84, 85, 971, 1385, 2589, 3086, 3185
AjnI	CCWGG	6 cut(s)	44, 574, 1031, 1378, 1389, 2877
AjuI	GAANNNNNNNTTGG	2 cut(s)	2889, 2921
AloI	GAACNNNNNNTCC	1 cut(s)	28
Alw21I	GWGCWC	2 cut(s)	125, 3204
Alw26I	GTCTC	2 cut(s)	1829, 3138
AlwI	GGATC	6 cut(s)	654, 767, 1914, 2070, 2201, 3131
AlwNI	CAGNNNCTG	3 cut(s)	131, 893, 1379
Ama87I	CYCGRG	1 cut(s)	249
ApoI	RAATTY	7 cut(s)	445, 683, 1719, 2322, 2469, 2563, 2762
ArsI	GACNNNNNNTTYG	4 cut(s)	2314, 2346, 3096, 3128
Asp700I	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
AspLEI	GCGC	1 cut(s)	37
AspS9I	GGNCC	5 cut(s)	254, 464, 817, 1366, 2315
AsuC2I	CCSGG	1 cut(s)	86
AsuHPI	GGTGA	3 cut(s)	2878, 2984, 3168
AsuII	TTCGAA	1 cut(s)	2816
AvaI	CYCGRG	1 cut(s)	249
AvaII	GGWCC	4 cut(s)	254, 464, 817, 1366
AxyI	CCTNAGG	1 cut(s)	1704
BalI	TGGCCA	5 cut(s)	810, 1013, 1389, 1410, 2785
BanII	GRGCYC	1 cut(s)	866
BauI	CACGAG	1 cut(s)	3135
BbsI	GAAGAC	3 cut(s)	153, 2024, 2360
Bbv12I	GWGCWC	2 cut(s)	125, 3204
BciT130I	CCWGG	6 cut(s)	46, 576, 1033, 1380, 1391, 2879
BciVI	GTATCC	2 cut(s)	915, 1590
BclI	TGATCA	2 cut(s)	628, 2443
BcnI	CCSGG	1 cut(s)	86
BcoDI	GTCTC	2 cut(s)	1829, 3138
BfaI	CTAG	4 cut(s)	2283, 2622, 2913, 2964
BfmI	CTRYAG	7 cut(s)	835, 1279, 1440, 2001, 2037, 2664, 3264
BfoI	RGCGCY	1 cut(s)	38
BfuAI	ACCTGC	1 cut(s)	1272
BfuI	GTATCC	2 cut(s)	915, 1590
BglI	GCCNNNNNGGC	1 cut(s)	59
BglII	AGATCT	4 cut(s)	245, 484, 1198, 1267
Bme1390I	CCNGG	7 cut(s)	46, 86, 576, 1033, 1380, 1391, 2879
Bme18I	GGWCC	4 cut(s)	254, 464, 817, 1366
BmeT110I	CYCGRG	1 cut(s)	249
BmgT120I	GGNCC	5 cut(s)	254, 464, 817, 1366, 2315
BmiI	GGNNCC	5 cut(s)	863, 1818, 2081, 2124, 3190
BmrFI	CCNGG	7 cut(s)	46, 86, 576, 1033, 1380, 1391, 2879
BmsI	GCATC	7 cut(s)	1663, 1870, 1946, 2077, 2911, 3019, 3152
BpiI	GAAGAC	3 cut(s)	153, 2024, 2360
BplI	GAGNNNNNCTC	2 cut(s)	3081, 3113
BpmI	CTGGAG	4 cut(s)	880, 1612, 2900, 3243
Bpu14I	TTCGAA	1 cut(s)	2816
BpuEI	CTTGAG	3 cut(s)	2072, 2870, 3165
BpuMI	CCSGG	1 cut(s)	86
BsaAI	YACGTR	1 cut(s)	207
BsaBI	GATNNNNATC	3 cut(s)	402, 1923, 2067
BsaJI	CCNNGG	7 cut(s)	44, 45, 57, 1031, 2210, 2925, 3141
BsaWI	WCCGGW	2 cut(s)	1294, 2951
BsaXI	ACNNNNNCTCC	6 cut(s)	1012, 1042, 1089, 1119, 2247, 2277
Bsc4I	CCNNNNNNNGG	9 cut(s)	31, 46, 84, 85, 971, 1385, 2589, 3086, 3185
Bse1I	ACTGG	9 cut(s)	752, 851, 863, 1368, 1411, 1595, 1790, 2133, 2731
Bse21I	CCTNAGG	1 cut(s)	1704
Bse3DI	GCAATG	2 cut(s)	1637, 1734
Bse8I	GATNNNNATC	3 cut(s)	402, 1923, 2067
BseBI	CCWGG	6 cut(s)	46, 576, 1033, 1380, 1391, 2879
BseDI	CCNNGG	7 cut(s)	44, 45, 57, 1031, 2210, 2925, 3141
BseGI	GGATG	9 cut(s)	110, 403, 1291, 1540, 1678, 1975, 2167, 2251, 2266
BseJI	GATNNNNATC	3 cut(s)	402, 1923, 2067
BseLI	CCNNNNNNNGG	9 cut(s)	31, 46, 84, 85, 971, 1385, 2589, 3086, 3185
BseMI	GCAATG	2 cut(s)	1637, 1734
BseMII	CTCAG	7 cut(s)	722, 806, 885, 1617, 2853, 2973, 3044
BseNI	ACTGG	9 cut(s)	752, 851, 863, 1368, 1411, 1595, 1790, 2133, 2731
BseRI	GAGGAG	2 cut(s)	1344, 2648
BseX3I	CGGCCG	1 cut(s)	3217
Bsh1236I	CGCG	2 cut(s)	1762, 2359
Bsh1285I	CGRYCG	1 cut(s)	3220
BsiEI	CGRYCG	1 cut(s)	3220
BsiHKAI	GWGCWC	2 cut(s)	125, 3204
BsiHKCI	CYCGRG	1 cut(s)	249
BsiSI	CCGG	3 cut(s)	85, 1295, 2952
BsiWI	CGTACG	1 cut(s)	2891
BslFI	GGGAC	1 cut(s)	1969
BslI	CCNNNNNNNGG	9 cut(s)	31, 46, 84, 85, 971, 1385, 2589, 3086, 3185
BsmAI	GTCTC	2 cut(s)	1829, 3138
BsmFI	GGGAC	1 cut(s)	1969
BsmI	GAATGC	3 cut(s)	1072, 1316, 2407
BsoBI	CYCGRG	1 cut(s)	249
Bsp119I	TTCGAA	1 cut(s)	2816
Bsp1286I	GDGCHC	3 cut(s)	125, 866, 3204
Bsp19I	CCATGG	1 cut(s)	57
BspCNI	CTCAG	7 cut(s)	721, 805, 886, 1618, 2854, 2974, 3043
BspFNI	CGCG	2 cut(s)	1762, 2359
BspHI	TCATGA	2 cut(s)	1521, 2392
BspLI	GGNNCC	5 cut(s)	863, 1818, 2081, 2124, 3190
BspMAI	CTGCAG	3 cut(s)	1283, 2005, 2041
BspMI	ACCTGC	1 cut(s)	1272
BspPI	GGATC	6 cut(s)	654, 767, 1914, 2070, 2201, 3131
BspQI	GCTCTTC	1 cut(s)	1996
BspT104I	TTCGAA	1 cut(s)	2816
BsrBI	CCGCTC	2 cut(s)	1743, 2258
BsrDI	GCAATG	2 cut(s)	1637, 1734
BsrI	ACTGG	9 cut(s)	752, 851, 863, 1368, 1411, 1595, 1790, 2133, 2731
BssECI	CCNNGG	7 cut(s)	44, 45, 57, 1031, 2210, 2925, 3141
BssSI	CACGAG	1 cut(s)	3135
BssT1I	CCWWGG	2 cut(s)	57, 2925
Bst2BI	CACGAG	1 cut(s)	3135
Bst2UI	CCWGG	6 cut(s)	46, 576, 1033, 1380, 1391, 2879
Bst4CI	ACNGT	7 cut(s)	322, 700, 1101, 2218, 2457, 2486, 2641
Bst6I	CTCTTC	3 cut(s)	294, 1827, 1996
BstAPI	GCANNNNNTGC	1 cut(s)	3213
BstBAI	YACGTR	1 cut(s)	207
BstBI	TTCGAA	1 cut(s)	2816
BstC8I	GCNNGC	3 cut(s)	1209, 2787, 3209
BstDEI	CTNAG	8 cut(s)	708, 792, 894, 1626, 1704, 2862, 2982, 3030
BstDSI	CCRYGG	2 cut(s)	57, 2210
BstENI	CCTNNNNNAGG	1 cut(s)	3084
BstF5I	GGATG	9 cut(s)	110, 403, 1291, 1540, 1678, 1975, 2167, 2251, 2266
BstFNI	CGCG	2 cut(s)	1762, 2359
BstH2I	RGCGCY	1 cut(s)	38
BstHHI	GCGC	1 cut(s)	37
BstMAI	GTCTC	2 cut(s)	1829, 3138
BstMCI	CGRYCG	1 cut(s)	3220
BstNI	CCWGG	6 cut(s)	46, 576, 1033, 1380, 1391, 2879
BstNSI	RCATGY	1 cut(s)	920
BstSCI	CCNGG	7 cut(s)	44, 84, 574, 1031, 1378, 1389, 2877
BstSFI	CTRYAG	7 cut(s)	835, 1279, 1440, 2001, 2037, 2664, 3264
BstSNI	TACGTA	1 cut(s)	207
BstUI	CGCG	2 cut(s)	1762, 2359
BstV2I	GAAGAC	3 cut(s)	153, 2024, 2360
BstX2I	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
BstXI	CCANNNNNNTGG	3 cut(s)	858, 1590, 2246
BstYI	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
BstZI	CGGCCG	1 cut(s)	3217
Bsu36I	CCTNAGG	1 cut(s)	1704
BsuI	GTATCC	2 cut(s)	915, 1590
BtgI	CCRYGG	2 cut(s)	57, 2210
BtgZI	GCGATG	3 cut(s)	175, 2316, 2996
BtsCI	GGATG	9 cut(s)	110, 403, 1291, 1540, 1678, 1975, 2167, 2251, 2266
BtsI	GCAGTG	2 cut(s)	2010, 3005
BtsIMutI	CAGTG	9 cut(s)	856, 961, 1404, 1461, 2010, 2140, 2453, 2736, 3005
BveI	ACCTGC	1 cut(s)	1272
Cac8I	GCNNGC	3 cut(s)	1209, 2787, 3209
CaiI	CAGNNNCTG	3 cut(s)	131, 893, 1379
CciI	TCATGA	2 cut(s)	1521, 2392
CciNI	GCGGCCGC	1 cut(s)	3217
CfoI	GCGC	1 cut(s)	37
Cfr13I	GGNCC	5 cut(s)	254, 464, 817, 1366, 2315
CseI	GACGC	3 cut(s)	2258, 2365, 3005
Csp6I	GTAC	9 cut(s)	1097, 1417, 1496, 1891, 2047, 2449, 2607, 2734, 2892
CviQI	GTAC	9 cut(s)	1097, 1417, 1496, 1891, 2047, 2449, 2607, 2734, 2892
DdeI	CTNAG	8 cut(s)	708, 792, 894, 1626, 1704, 2862, 2982, 3030
DraI	TTTAAA	2 cut(s)	507, 519
EaeI	YGGCCR	7 cut(s)	264, 808, 1011, 1387, 1408, 2783, 3217
EagI	CGGCCG	1 cut(s)	3217
Eam1104I	CTCTTC	3 cut(s)	294, 1827, 1996
EarI	CTCTTC	3 cut(s)	294, 1827, 1996
EclXI	CGGCCG	1 cut(s)	3217
Eco105I	TACGTA	1 cut(s)	207
Eco130I	CCWWGG	2 cut(s)	57, 2925
Eco24I	GRGCYC	1 cut(s)	866
Eco47I	GGWCC	4 cut(s)	254, 464, 817, 1366
Eco52I	CGGCCG	1 cut(s)	3217
Eco57I	CTGAAG	6 cut(s)	147, 303, 954, 1179, 1485, 2724
Eco81I	CCTNAGG	1 cut(s)	1704
Eco88I	CYCGRG	1 cut(s)	249
EcoNI	CCTNNNNNAGG	1 cut(s)	3084
EcoO109I	RGGNCCY	1 cut(s)	254
EcoRII	CCWGG	6 cut(s)	44, 574, 1031, 1378, 1389, 2877
EcoT14I	CCWWGG	2 cut(s)	57, 2925
EcoT38I	GRGCYC	1 cut(s)	866
ErhI	CCWWGG	2 cut(s)	57, 2925
FalI	AAGNNNNNCTT	2 cut(s)	2577, 2609
FaqI	GGGAC	1 cut(s)	1969
FauNDI	CATATG	2 cut(s)	472, 1585
FbaI	TGATCA	2 cut(s)	628, 2443
FblI	GTMKAC	1 cut(s)	2931
FokI	GGATG	9 cut(s)	97, 410, 1278, 1547, 1685, 1982, 2174, 2258, 2273
FriOI	GRGCYC	1 cut(s)	866
FspBI	CTAG	4 cut(s)	2283, 2622, 2913, 2964
GlaI	GCGC	1 cut(s)	36
GsuI	CTGGAG	4 cut(s)	880, 1612, 2900, 3243
HaeII	RGCGCY	1 cut(s)	38
HapII	CCGG	3 cut(s)	85, 1295, 2952
HgaI	GACGC	3 cut(s)	2258, 2365, 3005
HhaI	GCGC	1 cut(s)	37
Hin6I	GCGC	1 cut(s)	35
HinP1I	GCGC	1 cut(s)	35
HincII	GTYRAC	1 cut(s)	2554
HindII	GTYRAC	1 cut(s)	2554
HindIII	AAGCTT	3 cut(s)	542, 1073, 1235
HpaII	CCGG	3 cut(s)	85, 1295, 2952
HphI	GGTGA	3 cut(s)	2878, 2984, 3168
Hpy166II	GTNNAC	6 cut(s)	856, 1461, 2554, 2736, 2869, 2932
Hpy8I	GTNNAC	6 cut(s)	856, 1461, 2554, 2736, 2869, 2932
Hpy99I	CGWCG	1 cut(s)	2999
HpyCH4III	ACNGT	7 cut(s)	322, 700, 1101, 2218, 2457, 2486, 2641
HpyCH4IV	ACGT	2 cut(s)	206, 3111
HpyCH4V	TGCA	9 cut(s)	619, 887, 1281, 1676, 1727, 2003, 2039, 2648, 3010
HpyF3I	CTNAG	8 cut(s)	708, 792, 894, 1626, 1704, 2862, 2982, 3030
HpySE526I	ACGT	2 cut(s)	206, 3111
HspAI	GCGC	1 cut(s)	35
Ksp22I	TGATCA	2 cut(s)	628, 2443
LguI	GCTCTTC	1 cut(s)	1996
LmnI	GCTCC	7 cut(s)	42, 120, 861, 1381, 2128, 2255, 2686
LweI	GCATC	7 cut(s)	1663, 1870, 1946, 2077, 2911, 3019, 3152
MaeI	CTAG	4 cut(s)	2283, 2622, 2913, 2964
MaeII	ACGT	2 cut(s)	206, 3111
MaeIII	GTNAC	4 cut(s)	536, 1101, 1402, 2305
MbiI	CCGCTC	2 cut(s)	1743, 2258
MfeI	CAATTG	2 cut(s)	600, 1677
MflI	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
MhlI	GDGCHC	3 cut(s)	125, 866, 3204
MlsI	TGGCCA	5 cut(s)	810, 1013, 1389, 1410, 2785
MluNI	TGGCCA	5 cut(s)	810, 1013, 1389, 1410, 2785
MlyI	GAGTC	1 cut(s)	1817
MmeI	TCCRAC	4 cut(s)	64, 879, 989, 3033
Mox20I	TGGCCA	5 cut(s)	810, 1013, 1389, 1410, 2785
MroXI	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
MscI	TGGCCA	5 cut(s)	810, 1013, 1389, 1410, 2785
Msp20I	TGGCCA	5 cut(s)	810, 1013, 1389, 1410, 2785
MspA1I	CMGCKG	3 cut(s)	2539, 3222, 3263
MspI	CCGG	3 cut(s)	85, 1295, 2952
MspR9I	CCNGG	7 cut(s)	46, 86, 576, 1033, 1380, 1391, 2879
MunI	CAATTG	2 cut(s)	600, 1677
Mva1269I	GAATGC	3 cut(s)	1072, 1316, 2407
MvaI	CCWGG	6 cut(s)	46, 576, 1033, 1380, 1391, 2879
MvnI	CGCG	2 cut(s)	1762, 2359
NciI	CCSGG	1 cut(s)	86
NcoI	CCATGG	1 cut(s)	57
NdeI	CATATG	2 cut(s)	472, 1585
NlaIV	GGNNCC	5 cut(s)	863, 1818, 2081, 2124, 3190
NmeAIII	GCCGAG	1 cut(s)	3166
NmuCI	GTSAC	2 cut(s)	1101, 1402
NotI	GCGGCCGC	1 cut(s)	3217
NspI	RCATGY	1 cut(s)	920
NspV	TTCGAA	1 cut(s)	2816
PaeR7I	CTCGAG	1 cut(s)	249
PagI	TCATGA	2 cut(s)	1521, 2392
PasI	CCCWGGG	1 cut(s)	45
PciSI	GCTCTTC	1 cut(s)	1996
PcsI	WCGNNNNNNNCGW	1 cut(s)	3108
PctI	GAATGC	3 cut(s)	1072, 1316, 2407
PdmI	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
Pfl23II	CGTACG	1 cut(s)	2891
PflMI	CCANNNNNTGG	1 cut(s)	31
PfoI	TCCNGGA	2 cut(s)	1378, 2877
PleI	GAGTC	1 cut(s)	1816
PpsI	GAGTC	1 cut(s)	1816
Ppu21I	YACGTR	1 cut(s)	207
PpuMI	RGGWCCY	1 cut(s)	254
PsiI	TTATAA	2 cut(s)	558, 2141
Psp5II	RGGWCCY	1 cut(s)	254
Psp6I	CCWGG	6 cut(s)	44, 574, 1031, 1378, 1389, 2877
PspGI	CCWGG	6 cut(s)	44, 574, 1031, 1378, 1389, 2877
PspLI	CGTACG	1 cut(s)	2891
PspN4I	GGNNCC	5 cut(s)	863, 1818, 2081, 2124, 3190
PspPI	GGNCC	5 cut(s)	254, 464, 817, 1366, 2315
PspPPI	RGGWCCY	1 cut(s)	254
PstI	CTGCAG	3 cut(s)	1283, 2005, 2041
PstNI	CAGNNNCTG	3 cut(s)	131, 893, 1379
PsuI	RGATCY	7 cut(s)	245, 484, 646, 1198, 1267, 1906, 2206
PvuII	CAGCTG	1 cut(s)	3263
RsaI	GTAC	9 cut(s)	1098, 1418, 1497, 1892, 2048, 2450, 2608, 2735, 2893
RsaNI	GTAC	9 cut(s)	1097, 1417, 1496, 1891, 2047, 2449, 2607, 2734, 2892
SapI	GCTCTTC	1 cut(s)	1996
Sau96I	GGNCC	5 cut(s)	254, 464, 817, 1366, 2315
SchI	GAGTC	1 cut(s)	1817
ScrFI	CCNGG	7 cut(s)	46, 86, 576, 1033, 1380, 1391, 2879
SduI	GDGCHC	3 cut(s)	125, 866, 3204
SfaNI	GCATC	7 cut(s)	1663, 1870, 1946, 2077, 2911, 3019, 3152
SfcI	CTRYAG	7 cut(s)	835, 1279, 1440, 2001, 2037, 2664, 3264
Sfr274I	CTCGAG	1 cut(s)	249
SfuI	TTCGAA	1 cut(s)	2816
SinI	GGWCC	4 cut(s)	254, 464, 817, 1366
SlaI	CTCGAG	1 cut(s)	249
SmlI	CTYRAG	4 cut(s)	249, 2051, 2849, 3180
SmoI	CTYRAG	4 cut(s)	249, 2051, 2849, 3180
SnaBI	TACGTA	1 cut(s)	207
SspI	AATATT	2 cut(s)	1513, 1614
SspMI	CTAG	4 cut(s)	2283, 2622, 2913, 2964
StyD4I	CCNGG	7 cut(s)	44, 84, 574, 1031, 1378, 1389, 2877
StyI	CCWWGG	2 cut(s)	57, 2925
TaaI	ACNGT	7 cut(s)	322, 700, 1101, 2218, 2457, 2486, 2641
TaiI	ACGT	2 cut(s)	209, 3114
TaqI	TCGA	7 cut(s)	250, 640, 923, 1856, 1902, 2816, 3102
TatI	WGTACW	3 cut(s)	1096, 1495, 2733
TauI	GCSGC	7 cut(s)	23, 56, 269, 1743, 1765, 3219, 3222
TscAI	CASTG	9 cut(s)	863, 961, 1411, 1468, 2010, 2140, 2460, 2743, 3012
TseFI	GTSAC	2 cut(s)	1101, 1402
Tsp45I	GTSAC	2 cut(s)	1101, 1402
TspGWI	ACGGA	5 cut(s)	1434, 2227, 2318, 2715, 2909
TspRI	CASTG	9 cut(s)	863, 961, 1411, 1468, 2010, 2140, 2460, 2743, 3012
Van91I	CCANNNNNTGG	1 cut(s)	31
VpaK11BI	GGWCC	4 cut(s)	254, 464, 817, 1366
XagI	CCTNNNNNAGG	1 cut(s)	3084
XapI	RAATTY	7 cut(s)	445, 683, 1719, 2322, 2469, 2563, 2762
XbaI	TCTAGA	1 cut(s)	2963
XceI	RCATGY	1 cut(s)	920
XcmI	CCANNNNNNNNNTGG	3 cut(s)	946, 1054, 1376
XhoI	CTCGAG	1 cut(s)	249
XmiI	GTMKAC	1 cut(s)	2931
XmnI	GAANNNNTTC	3 cut(s)	1178, 1653, 2844
XspI	CTAG	4 cut(s)	2283, 2622, 2913, 2964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.