Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0007310

Physical Location & Seq

Genomic Coordinates Reverse (-)

19116107 .. 19128628

Gene Structure 12522 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007623

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3267 bp

ATGAATTTCAAATCCTTGGCTTCAAGCTCATCCAGAGAAGCCGCCACAATGGCCATGATTCAAACCACTCCATACAAGGTTCTGTCGCAAAGAACTCACTCAACTTTTCGGGGCACAGCTTCTGTTGGCTTGTTTTATTTCCGAGGAAGGTCTTCTGCGAAAGAATTCTCCCTCTTTAATATCAGACACTCTTCTACGCATTCCAGTGATGAGTTTGCTTCTTCCTCAAAGCGAAGGTCTCGGGGACCTGTTATGGCTGCAAAGAAAGCGGCTGAAGGGGGAAAGCAGCAGGATGGAAAATACAAGCACACGGTCAATCTGCCAAAGACATCATTTGGCATGAGAGCGAATTCTTCAACACGAGAGCCTGAACTTCAGAAAATATGGGAGGACAATCAAGTGTTCAAGAGAGTAGTTGGTAAAAATACAGGGGAAAACTTCATTCTTCATGATGGTCCTCCATACGCCAACGGTGATCTGCACATCGGCCATGCTCTAAATAAAATTTTGAAGGATATGATTAATCGTTATAAGCTTCTTCAAAATTATAAAGTTCATTATGTGCCTGGTTGGGATTGTCATGGCTTACCAATTGAATTGAAAGTCCTGCAATCCCTTGATCAAGCTGCTCGAAAGGATCTCACACCAATAAAGTTGAGAGCGAAGGCAGCTAAGTTTGCCAAGCAAACAGTTAAAAATCAGATGGAATCATTTAAGCGTTATGGGATATGGGCAGACTGGAATAATCCCTACCTGACTCTTGATCCAGAATATGAAGCTGCTCAGATAGAAGTGTTTGGTCAGATGGTCCTTCAAGGTTTTATCTACAGAGGCAGGAAACCAGTTCACTGGAGTCCCTCATCACGAACTGCCCTTGCAGAGGCTGAGTTGGAGTATCCGGAGAGTCATGTTTCAAGGAGTATCTATACCGCTTTCAAATTAGTGAGTGCACCTCCAACTTCAGGTGGCTTATTGAATGAGTATCCAGATGTTTGCTTGGCCATTTGGACAACTACTCCCTGGACTATTCCAGCAAATGCCGCGGTTGCAGTGAATGCCAAGCTTATATATGCCATTGTTGAAGTACAGTCAGTCTCCGAAGATGTTTCTTCATCTGCCGGAAATAGCAAGCAAACGCCTGCTAATTTTCTCAAGGAAGAAAAGAAGCCTTTCCTCATTGTAGCTTCAGATCTTGTACCAACTTTAGAAGCAAAATGGGGCCTCAAGCTTGTTGTTAGGAAAAGGGTGTCAGGTTCAGATCTTGAGAACTGCAGGTATATCCATCCAGTATTTAATAGGGAATGTTCAGTTGTGATTGGAGGAGATTATATCACAACAGAGTCGGGAACTGGACTGGTCCATACAGCTCCTGGACATGGTCAGGAAGATTATGTCACTGGCCTTAAGTATGGACTGCCTATTCTTTCCCCAGTAGATGATGATGGAAAGTTCACAGAAGAAGCTGGAAAATTTTGTGGGCTTGATGTACTTGCTGATGGTAATATTGCTGTTGTGAAATACTTGGATGAGCATTTGTCACTGATCATGGAAGAATCCTACAAACATAAGTATCCATATGATTGGAGAACGAAAAAGCCCACTATATTTAGGGCAACTGAGCAATGGTTTGCATCAGTGGAAGGCTTTCGTGAGGCTGTTATGGATGCAATTGGCAATGTAAAATGGATTCCAGCTAAGGCAGAAAACAGAATATCGGCAATGACTTCTAGCCGCTCTGATTGGTGTATATCGCGGCAGAGGACGTGGGGTGTTCCAATTCCTGTCTTTTATCACCTCCAGTCAAAGGAACCTTTAATGAATGAGGAGACTATTGATCATATCAAGTCTATAATTTCTGAAAAGGGTAGCGATGCATGGTGGTACATGAAGGTAGAGGATCTTCTCCCTAGTAAATATCGTGATAAAGCATCTGAATATGAAAAGGGAACTGACACAATGGATGTATGGTTCGACTCAGGCTCTTCTTGGGCTGCAGTTTTAGGAAAAAGAAAAGGCCTTAGTCTCCCTGCAGACTTGTACCTTGAAGGTATGGATCAGCATCGTGGGTGGTTCCAGAGTTCTTTGTTAACAAGTGTTGCTACAAAAGGAAAGGCTCCATATTCCAGTGTCATAACACATGGATTTGTATTGGATGAGAAGGGTTTAAAGATGAGCAAATCTCAGGGTAACGTTGTAGACCCAAGAACTGTGATTGAAGGAGGGAAGAACCAAAAGGATGGCTATGGAGCTGATGTTCTGCGTCTCTGGGTTTCCAGTATAGATTATACAGGCGATGTTATGATAGGTGCTCAAGTTCTCCGTCAAATGTCAGATATATATAGGAAGCTACGAGGAACATTGAGATACCTTTTGGGAAATCTGCATGATTGGCATGCTGATAATGCTATTTCATACCATGATCTTCCCATGATTGATCAGCATGCACTGTTTCAACTTGAAAATTTTGTAAACAACAGCAGAGAGTGCTATGAAAGTTATCAGTTCTTTAAGATATTTCAGATCATACAGCGGTTTGTTATTGTTGACCTATCAAATTTCTACTTTGATATTGCCAAAGATCGTTTATATGTTGGGGGCACTACAAGTTTTACTAGGAGAAGTTGTCAGACGGTTCTTGCAGAACTGCTCCTTTCTATTGTGAGAGTGATTGCTCCAATATTGCCTCATTTGGCTGAGGATGTATGGCAAAATCTTCCATTCCAGTACACTGACAAAAATGGCTCTGTTGCGGAATTTGTTTTTGAATCAAGATGGCCAGCTTCAAACGAAACATGGCTCTCTCTTTCTAAAGAGGAAACTGATTTCTGGACTAAAATTCTTGAGCTGAGAACCGAGGTGAATAAAGTGTTGGAGATTGCTCGTACAACAAAGTTAATTGGCTCTAGCTTAGATGCCAAGGTCTACCTCCATACATGGGATTCTGGTCTGGCCTCAAGATTGGTTCAAATGTCTGCAGCCAACAACGATGCAGACACATTGAATCGGATATTCATAACTTCCCAGGCAGAGGTTCTTCCATCCTTAGAAGATGACTGGATTGCGAAAATACCACATAAAGGAGAATGTCAAGTTGACGAAAGCATTAAAGTTTGGATTGGTGTGTCTCGTGCTGAGGGCCTAAAGTGTGAAAGATGCTGGAACTATTCACCTCAGGTTGGTTCCTTTCCGGACCACAGCACCCTTTGCAGCCGCTGCTATGATGTGGTTGATATTCAGCAAGCTGCAGCTGAAGCTGTTGTCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 zf-FPG_IleRS

Protein Analysis

1089

Amino Acids

122.63

Weight (kDa)

7.36

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	125 - 767	2.3e-133	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	154 - 298	5.4e-13	tRNA synthetases class I (M)
Anticodon_1	PF08264	812 - 975	5.1e-30	Anticodon-binding domain of tRNA ligase
zf-FPG_IleRS	PF06827	1046 - 1075	4.9e-08	Zinc finger found in FPG and IleRS

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0007310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49030 AT5G49030 AT5G49030
fragaria_vesca	FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060 FvH4_4g18060
malus_domestica	MD13G1168400.v1.1 MD16G1168800.v1.1
prunus_persica	Prupe.1G136500_v2.0.a1 Prupe.1G136500_v2.0.a1
pyrus_communis	pycom13g14400 pycom16g14210
rosa_chinensis	RchiOBHm_Chr4g0422271
rosa_laevigata	RLG00000007623
rosa_multiflora	Rmu_sc0013245.1_g000018
rosa_roxburghii	Rroxscaffold_5G00364380
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238300 Rh4BG241400 Rh4CG253300 Rh4DG237000
rosa_wichuraiana	Rw4G020620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	531, 549
Acc36I	ACCTGC	1 cut(s)	1263
AccBSI	CCGCTC	1 cut(s)	1734
AccI	GTMKAC	2 cut(s)	2196, 2922
AccII	CGCG	2 cut(s)	1043, 1753
AccIII	TCCGGA	2 cut(s)	898, 3187
AclI	AACGTT	1 cut(s)	2190
AclWI	GGATC	4 cut(s)	645, 758, 1905, 2061
AcoI	YGGCCR	4 cut(s)	51, 487, 999, 2774
AcsI	RAATTY	9 cut(s)	4, 164, 349, 504, 1469, 2461, 2554, 2753, 2835
AcuI	CTGAAG	4 cut(s)	294, 359, 945, 1170
AfaI	GTAC	7 cut(s)	1086, 1197, 1488, 1883, 2039, 2726, 2884
AfiI	CCNNNNNNNGG	8 cut(s)	880, 962, 1376, 1804, 2935, 3028, 3077, 3176
AflII	CTTAAG	1 cut(s)	1403
AjiI	CACGTC	1 cut(s)	1764
AjnI	CCWGG	4 cut(s)	565, 1019, 1369, 3021
AjuI	GAANNNNNNNTTGG	2 cut(s)	2852, 2884
AloI	GAACNNNNNNTCC	4 cut(s)	1952, 1984, 2238, 2270
Alw21I	GWGCWC	2 cut(s)	952, 2311
Alw26I	GTCTC	6 cut(s)	243, 1099, 1820, 2027, 2267, 3129
Alw44I	GTGCAC	1 cut(s)	948
AlwI	GGATC	4 cut(s)	645, 758, 1905, 2061
AlwNI	CAGNNNCTG	4 cut(s)	122, 884, 1370, 3213
Ama87I	CYCGRG	1 cut(s)	240
Aor13HI	TCCGGA	2 cut(s)	898, 3187
AoxI	GGCC	9 cut(s)	51, 487, 999, 1219, 1399, 2014, 2774, 2949, 3136
ApaLI	GTGCAC	1 cut(s)	948
ApoI	RAATTY	9 cut(s)	4, 164, 349, 504, 1469, 2461, 2554, 2753, 2835
ArsI	GACNNNNNNTTYG	2 cut(s)	221, 253
AseI	ATTAAT	1 cut(s)	522
Asp700I	GAANNNNTTC	4 cut(s)	151, 164, 1169, 1644
AspS9I	GGNCC	7 cut(s)	245, 455, 808, 1219, 1357, 3136, 3190
AsuHPI	GGTGA	4 cut(s)	485, 1784, 2869, 3159
AvaI	CYCGRG	1 cut(s)	240
AvaII	GGWCC	5 cut(s)	245, 455, 808, 1357, 3190
AxyI	CCTNAGG	1 cut(s)	3171
BaeGI	GKGCMC	3 cut(s)	116, 952, 2600
BaeI	ACNNNNGTAYC	2 cut(s)	1553, 1586
BalI	TGGCCA	3 cut(s)	53, 1001, 2776
BarI	GAAGNNNNNNTAC	2 cut(s)	1884, 1916
BauI	CACGAG	2 cut(s)	360, 3126
BbsI	GAAGAC	1 cut(s)	144
Bbv12I	GWGCWC	2 cut(s)	952, 2311
BbvCI	CCTCAGC	2 cut(s)	2694, 3132
BciT130I	CCWGG	4 cut(s)	567, 1021, 1371, 3023
BciVI	GTATCC	3 cut(s)	906, 993, 1581
BclI	TGATCA	4 cut(s)	619, 1542, 1834, 2434
BcoDI	GTCTC	6 cut(s)	243, 1099, 1820, 2027, 2267, 3129
BfaI	CTAG	4 cut(s)	1728, 1908, 2613, 2904
BfmI	CTRYAG	6 cut(s)	826, 1270, 1992, 2028, 2973, 3243
BfrI	CTTAAG	1 cut(s)	1403
BfuAI	ACCTGC	1 cut(s)	1263
BfuI	GTATCC	3 cut(s)	906, 993, 1581
BglI	GCCNNNNNGGC	1 cut(s)	50
BglII	AGATCT	2 cut(s)	1189, 1258
Bme1390I	CCNGG	4 cut(s)	567, 1021, 1371, 3023
Bme18I	GGWCC	5 cut(s)	245, 455, 808, 1357, 3190
BmeT110I	CYCGRG	1 cut(s)	240
BmgBI	CACGTC	1 cut(s)	1764
BmgT120I	GGNCC	7 cut(s)	245, 455, 808, 1219, 1357, 3136, 3190
BmiI	GGNNCC	6 cut(s)	246, 1220, 1809, 2072, 2115, 3181
BmrFI	CCNGG	4 cut(s)	567, 1021, 1371, 3023
BmrI	ACTGGG	1 cut(s)	1424
BmsI	GCATC	8 cut(s)	1640, 1654, 1861, 1937, 2068, 2902, 2977, 3143
BmuI	ACTGGG	1 cut(s)	1424
BpiI	GAAGAC	1 cut(s)	144
BplI	GAGNNNNNCTC	4 cut(s)	937, 969, 2164, 2196
BpmI	CTGGAG	2 cut(s)	871, 1781
Bpu10I	CCTNAGC	3 cut(s)	1695, 2694, 3132
BpuEI	CTTGAG	6 cut(s)	1136, 1208, 1283, 2295, 2861, 2938
BsaBI	GATNNNNATC	1 cut(s)	2058
BsaI	GGTCTC	1 cut(s)	243
BsaJI	CCNNGG	7 cut(s)	15, 142, 1019, 1041, 2853, 2916, 3021
BsaWI	WCCGGW	2 cut(s)	898, 3187
BsaXI	ACNNNNNCTCC	6 cut(s)	1000, 1030, 2238, 2268, 2608, 2638
Bsc4I	CCNNNNNNNGG	8 cut(s)	880, 962, 1376, 1804, 2935, 3028, 3077, 3176
Bse21I	CCTNAGG	1 cut(s)	3171
Bse3DI	GCAATG	3 cut(s)	1628, 1681, 1725
Bse8I	GATNNNNATC	1 cut(s)	2058
BseAI	TCCGGA	2 cut(s)	898, 3187
BseBI	CCWGG	4 cut(s)	567, 1021, 1371, 3023
BseDI	CCNNGG	7 cut(s)	15, 142, 1019, 1041, 2853, 2916, 3021
BseJI	GATNNNNATC	1 cut(s)	2058
BseLI	CCNNNNNNNGG	8 cut(s)	880, 962, 1376, 1804, 2935, 3028, 3077, 3176
BseMI	GCAATG	3 cut(s)	1628, 1681, 1725
BseMII	CTCAG	9 cut(s)	797, 876, 1608, 1989, 2195, 2685, 2837, 3123, 3185
BseRI	GAGGAG	2 cut(s)	1335, 1838
BseSI	GKGCMC	3 cut(s)	116, 952, 2600
BsgI	GTGCAG	1 cut(s)	464
Bsh1236I	CGCG	2 cut(s)	1043, 1753
BshFI	GGCC	9 cut(s)	53, 489, 1001, 1221, 1401, 2016, 2776, 2951, 3138
BsiHKAI	GWGCWC	2 cut(s)	952, 2311
BsiHKCI	CYCGRG	1 cut(s)	240
BsiSI	CCGG	3 cut(s)	899, 1119, 3188
BslFI	GGGAC	2 cut(s)	258, 840
BslI	CCNNNNNNNGG	8 cut(s)	880, 962, 1376, 1804, 2935, 3028, 3077, 3176
BsmAI	GTCTC	6 cut(s)	243, 1099, 1820, 2027, 2267, 3129
BsmBI	CGTCTC	1 cut(s)	2267
BsmFI	GGGAC	2 cut(s)	258, 840
BsmI	GAATGC	2 cut(s)	199, 1060
BsnI	GGCC	9 cut(s)	53, 489, 1001, 1221, 1401, 2016, 2776, 2951, 3138
Bso31I	GGTCTC	1 cut(s)	243
BsoBI	CYCGRG	1 cut(s)	240
Bsp1286I	GDGCHC	4 cut(s)	116, 952, 2311, 2600
Bsp13I	TCCGGA	2 cut(s)	898, 3187
BspANI	GGCC	9 cut(s)	53, 489, 1001, 1221, 1401, 2016, 2776, 2951, 3138
BspCNI	CTCAG	9 cut(s)	796, 877, 1609, 1988, 2194, 2686, 2838, 3124, 3184
BspEI	TCCGGA	2 cut(s)	898, 3187
BspFNI	CGCG	2 cut(s)	1043, 1753
BspHI	TCATGA	1 cut(s)	448
BspLI	GGNNCC	6 cut(s)	246, 1220, 1809, 2072, 2115, 3181
BspMAI	CTGCAG	5 cut(s)	1274, 1996, 2032, 2977, 3247
BspMI	ACCTGC	1 cut(s)	1263
BspPI	GGATC	4 cut(s)	645, 758, 1905, 2061
BspQI	GCTCTTC	1 cut(s)	1987
BspTI	CTTAAG	1 cut(s)	1403
BspTNI	GGTCTC	1 cut(s)	243
BsrBI	CCGCTC	1 cut(s)	1734
BsrDI	GCAATG	3 cut(s)	1628, 1681, 1725
BssECI	CCNNGG	7 cut(s)	15, 142, 1019, 1041, 2853, 2916, 3021
BssSI	CACGAG	2 cut(s)	360, 3126
BssT1I	CCWWGG	2 cut(s)	15, 2916
Bst2BI	CACGAG	2 cut(s)	360, 3126
Bst2UI	CCWGG	4 cut(s)	567, 1021, 1371, 3023
Bst4CI	ACNGT	7 cut(s)	313, 473, 691, 1089, 2209, 2448, 2632
Bst6I	CTCTTC	2 cut(s)	196, 1987
BstAFI	CTTAAG	1 cut(s)	1403
BstAPI	GCANNNNNTGC	4 cut(s)	1055, 2484, 3204, 3213
BstC8I	GCNNGC	6 cut(s)	1130, 1140, 2394, 2442, 2778, 3240
BstDSI	CCRYGG	1 cut(s)	1041
BstENI	CCTNNNNNAGG	1 cut(s)	878
BstFNI	CGCG	2 cut(s)	1043, 1753
BstMAI	GTCTC	6 cut(s)	243, 1099, 1820, 2027, 2267, 3129
BstNI	CCWGG	4 cut(s)	567, 1021, 1371, 3023
BstNSI	RCATGY	2 cut(s)	2396, 2444
BstSCI	CCNGG	4 cut(s)	565, 1019, 1369, 3021
BstSFI	CTRYAG	6 cut(s)	826, 1270, 1992, 2028, 2973, 3243
BstSLI	GKGCMC	3 cut(s)	116, 952, 2600
BstUI	CGCG	2 cut(s)	1043, 1753
BstV2I	GAAGAC	1 cut(s)	144
BstX2I	RGATCY	4 cut(s)	637, 1189, 1258, 1897
BstXI	CCANNNNNNTGG	3 cut(s)	849, 1581, 2237
BstYI	RGATCY	4 cut(s)	637, 1189, 1258, 1897
Bsu36I	CCTNAGG	1 cut(s)	3171
BsuI	GTATCC	3 cut(s)	906, 993, 1581
BsuRI	GGCC	9 cut(s)	53, 489, 1001, 1221, 1401, 2016, 2776, 2951, 3138
BtgI	CCRYGG	1 cut(s)	1041
BtgZI	GCGATG	2 cut(s)	1884, 2307
BtrI	CACGTC	1 cut(s)	1764
BtsI	GCAGTG	1 cut(s)	1056
BtsIMutI	CAGTG	9 cut(s)	211, 847, 1056, 1395, 1538, 1641, 2131, 2444, 2727
BveI	ACCTGC	1 cut(s)	1263
Cac8I	GCNNGC	6 cut(s)	1130, 1140, 2394, 2442, 2778, 3240
CaiI	CAGNNNCTG	4 cut(s)	122, 884, 1370, 3213
CciI	TCATGA	1 cut(s)	448
Cfr13I	GGNCC	7 cut(s)	245, 455, 808, 1219, 1357, 3136, 3190
Cfr42I	CCGCGG	1 cut(s)	1044
CseI	GACGC	1 cut(s)	2249
Csp6I	GTAC	7 cut(s)	1085, 1196, 1487, 1882, 2038, 2725, 2883
CviQI	GTAC	7 cut(s)	1085, 1196, 1487, 1882, 2038, 2725, 2883
DraI	TTTAAA	1 cut(s)	2166
EaeI	YGGCCR	4 cut(s)	51, 487, 999, 2774
Eam1104I	CTCTTC	2 cut(s)	196, 1987
EarI	CTCTTC	2 cut(s)	196, 1987
Eco130I	CCWWGG	2 cut(s)	15, 2916
Eco147I	AGGCCT	1 cut(s)	2016
Eco31I	GGTCTC	1 cut(s)	243
Eco47I	GGWCC	5 cut(s)	245, 455, 808, 1357, 3190
Eco57I	CTGAAG	4 cut(s)	294, 359, 945, 1170
Eco81I	CCTNAGG	1 cut(s)	3171
Eco88I	CYCGRG	1 cut(s)	240
EcoNI	CCTNNNNNAGG	1 cut(s)	878
EcoO109I	RGGNCCY	3 cut(s)	245, 1219, 3136
EcoRI	GAATTC	2 cut(s)	164, 349
EcoRII	CCWGG	4 cut(s)	565, 1019, 1369, 3021
EcoT14I	CCWWGG	2 cut(s)	15, 2916
EcoT22I	ATGCAT	1 cut(s)	1876
ErhI	CCWWGG	2 cut(s)	15, 2916
Esp3I	CGTCTC	1 cut(s)	2267
FaqI	GGGAC	2 cut(s)	258, 840
FauNDI	CATATG	1 cut(s)	1576
FbaI	TGATCA	4 cut(s)	619, 1542, 1834, 2434
FblI	GTMKAC	2 cut(s)	2196, 2922
FspBI	CTAG	4 cut(s)	1728, 1908, 2613, 2904
GsuI	CTGGAG	2 cut(s)	871, 1781
HaeIII	GGCC	9 cut(s)	53, 489, 1001, 1221, 1401, 2016, 2776, 2951, 3138
HapII	CCGG	3 cut(s)	899, 1119, 3188
HgaI	GACGC	1 cut(s)	2249
HincII	GTYRAC	3 cut(s)	2088, 2545, 3094
HindII	GTYRAC	3 cut(s)	2088, 2545, 3094
HindIII	AAGCTT	3 cut(s)	533, 1061, 1226
HpaI	GTTAAC	1 cut(s)	2088
HpaII	CCGG	3 cut(s)	899, 1119, 3188
HphI	GGTGA	4 cut(s)	485, 1784, 2869, 3159
HpyCH4III	ACNGT	7 cut(s)	313, 473, 691, 1089, 2209, 2448, 2632
HpyCH4IV	ACGT	2 cut(s)	1763, 2190
HpySE526I	ACGT	2 cut(s)	1763, 2190
Kpn2I	TCCGGA	2 cut(s)	898, 3187
Ksp22I	TGATCA	4 cut(s)	619, 1542, 1834, 2434
KspAI	GTTAAC	1 cut(s)	2088
KspI	CCGCGG	1 cut(s)	1044
LguI	GCTCTTC	1 cut(s)	1987
LmnI	GCTCC	5 cut(s)	1372, 2119, 2246, 2652, 2677
LweI	GCATC	8 cut(s)	1640, 1654, 1861, 1937, 2068, 2902, 2977, 3143
MaeI	CTAG	4 cut(s)	1728, 1908, 2613, 2904
MaeII	ACGT	2 cut(s)	1763, 2190
MaeIII	GTNAC	3 cut(s)	1393, 1536, 2186
MbiI	CCGCTC	1 cut(s)	1734
MfeI	CAATTG	2 cut(s)	591, 1668
MflI	RGATCY	4 cut(s)	637, 1189, 1258, 1897
MhlI	GDGCHC	4 cut(s)	116, 952, 2311, 2600
MlsI	TGGCCA	3 cut(s)	53, 1001, 2776
MluNI	TGGCCA	3 cut(s)	53, 1001, 2776
MlyI	GAGTC	5 cut(s)	751, 862, 913, 1349, 1967
MmeI	TCCRAC	3 cut(s)	870, 980, 2850
Mox20I	TGGCCA	3 cut(s)	53, 1001, 2776
Mph1103I	ATGCAT	1 cut(s)	1876
MroI	TCCGGA	2 cut(s)	898, 3187
MroXI	GAANNNNTTC	4 cut(s)	151, 164, 1169, 1644
MscI	TGGCCA	3 cut(s)	53, 1001, 2776
MslI	CAYNNNNRTG	1 cut(s)	204
Msp20I	TGGCCA	3 cut(s)	53, 1001, 2776
MspA1I	CMGCKG	4 cut(s)	1043, 2530, 3213, 3248
MspCI	CTTAAG	1 cut(s)	1403
MspI	CCGG	3 cut(s)	899, 1119, 3188
MspR9I	CCNGG	4 cut(s)	567, 1021, 1371, 3023
MunI	CAATTG	2 cut(s)	591, 1668
Mva1269I	GAATGC	2 cut(s)	199, 1060
MvaI	CCWGG	4 cut(s)	567, 1021, 1371, 3023
MvnI	CGCG	2 cut(s)	1043, 1753
NdeI	CATATG	1 cut(s)	1576
NlaIV	GGNNCC	6 cut(s)	246, 1220, 1809, 2072, 2115, 3181
NmuCI	GTSAC	2 cut(s)	1393, 1536
NsiI	ATGCAT	1 cut(s)	1876
NspI	RCATGY	2 cut(s)	2396, 2444
PaeI	GCATGC	2 cut(s)	2396, 2444
PagI	TCATGA	1 cut(s)	448
PceI	AGGCCT	1 cut(s)	2016
PciSI	GCTCTTC	1 cut(s)	1987
PctI	GAATGC	2 cut(s)	199, 1060
PdmI	GAANNNNTTC	4 cut(s)	151, 164, 1169, 1644
PfeI	GAWTC	7 cut(s)	58, 707, 1553, 1687, 2765, 2939, 3001
PflFI	GACNNNGTC	1 cut(s)	1377
PfoI	TCCNGGA	1 cut(s)	1369
PleI	GAGTC	5 cut(s)	751, 861, 912, 1348, 1967
PpsI	GAGTC	5 cut(s)	751, 861, 912, 1348, 1967
PpuMI	RGGWCCY	1 cut(s)	245
PshBI	ATTAAT	1 cut(s)	522
PsiI	TTATAA	2 cut(s)	531, 549
Psp1406I	AACGTT	1 cut(s)	2190
Psp5II	RGGWCCY	1 cut(s)	245
Psp6I	CCWGG	4 cut(s)	565, 1019, 1369, 3021
PspGI	CCWGG	4 cut(s)	565, 1019, 1369, 3021
PspN4I	GGNNCC	6 cut(s)	246, 1220, 1809, 2072, 2115, 3181
PspPI	GGNCC	7 cut(s)	245, 455, 808, 1219, 1357, 3136, 3190
PspPPI	RGGWCCY	1 cut(s)	245
PstI	CTGCAG	5 cut(s)	1274, 1996, 2032, 2977, 3247
PstNI	CAGNNNCTG	4 cut(s)	122, 884, 1370, 3213
PsuI	RGATCY	4 cut(s)	637, 1189, 1258, 1897
PsyI	GACNNNGTC	1 cut(s)	1377
PvuII	CAGCTG	1 cut(s)	3248
RsaI	GTAC	7 cut(s)	1086, 1197, 1488, 1883, 2039, 2726, 2884
RsaNI	GTAC	7 cut(s)	1085, 1196, 1487, 1882, 2038, 2725, 2883
RseI	CAYNNNNRTG	1 cut(s)	204
SacII	CCGCGG	1 cut(s)	1044
SapI	GCTCTTC	1 cut(s)	1987
Sau96I	GGNCC	7 cut(s)	245, 455, 808, 1219, 1357, 3136, 3190
SchI	GAGTC	5 cut(s)	751, 862, 913, 1349, 1967
ScrFI	CCNGG	4 cut(s)	567, 1021, 1371, 3023
SduI	GDGCHC	4 cut(s)	116, 952, 2311, 2600
SfaNI	GCATC	8 cut(s)	1640, 1654, 1861, 1937, 2068, 2902, 2977, 3143
SfcI	CTRYAG	6 cut(s)	826, 1270, 1992, 2028, 2973, 3243
Sfr303I	CCGCGG	1 cut(s)	1044
SgrBI	CCGCGG	1 cut(s)	1044
SinI	GGWCC	5 cut(s)	245, 455, 808, 1357, 3190
SmiMI	CAYNNNNRTG	1 cut(s)	204
SmlI	CTYRAG	7 cut(s)	1151, 1223, 1262, 1403, 2310, 2840, 2953
SmoI	CTYRAG	7 cut(s)	1151, 1223, 1262, 1403, 2310, 2840, 2953
SphI	GCATGC	2 cut(s)	2396, 2444
SseBI	AGGCCT	1 cut(s)	2016
SspI	AATATT	2 cut(s)	1504, 2679
SspMI	CTAG	4 cut(s)	1728, 1908, 2613, 2904
StuI	AGGCCT	1 cut(s)	2016
StyD4I	CCNGG	4 cut(s)	565, 1019, 1369, 3021
StyI	CCWWGG	2 cut(s)	15, 2916
TaaI	ACNGT	7 cut(s)	313, 473, 691, 1089, 2209, 2448, 2632
TaiI	ACGT	2 cut(s)	1766, 2193
TaqI	TCGA	2 cut(s)	631, 1971
TatI	WGTACW	3 cut(s)	1084, 1486, 2724
TauI	GCSGC	6 cut(s)	44, 272, 1043, 1734, 1756, 3213
TfiI	GAWTC	7 cut(s)	58, 707, 1553, 1687, 2765, 2939, 3001
TscAI	CASTG	9 cut(s)	211, 854, 1056, 1402, 1545, 1641, 2131, 2451, 2734
TseFI	GTSAC	2 cut(s)	1393, 1536
Tsp45I	GTSAC	2 cut(s)	1393, 1536
TspGWI	ACGGA	1 cut(s)	2309
TspRI	CASTG	9 cut(s)	211, 854, 1056, 1402, 1545, 1641, 2131, 2451, 2734
Tth111I	GACNNNGTC	1 cut(s)	1377
Vha464I	CTTAAG	1 cut(s)	1403
VneI	GTGCAC	1 cut(s)	948
VpaK11BI	GGWCC	5 cut(s)	245, 455, 808, 1357, 3190
VspI	ATTAAT	1 cut(s)	522
XagI	CCTNNNNNAGG	1 cut(s)	878
XapI	RAATTY	9 cut(s)	4, 164, 349, 504, 1469, 2461, 2554, 2753, 2835
XceI	RCATGY	2 cut(s)	2396, 2444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1367
XmiI	GTMKAC	2 cut(s)	2196, 2922
XmnI	GAANNNNTTC	4 cut(s)	151, 164, 1169, 1644
XspI	CTAG	4 cut(s)	1728, 1908, 2613, 2904
Zsp2I	ATGCAT	1 cut(s)	1876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.