Rh4BG037400

Electron transfer flavoprotein subunit beta

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011199
Physical Location & Seq
Reverse (-)
6656445 .. 6660563
4119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG037400.1

Sequence Viewer

Length: 885 bp
ATGCCGTTACGAAACATATCTCTTCTTCTCACTCTCATTCTCTCAAGTCTGTCTCACCATAACAGATCATCCAACTTCAATCTCTTTAACCCATTACCATTTCACTCTCACACCCACCACCGCTCAAGCATGAAGATAATGGTAGCAATCAAACGCGTGGTCGACTACGCCGTCAAAATCCGAGTCAAGTCCGACAAGAGCGGCGTGGAGACCCAGAACGTGAAGATGTCGATGAACCCATTTTGCGAGATCGCTTTGGAAGAGGCCCTTCGGATCAAAGAGTCGGGCTTGGCCTCCGAGGTCGTGGCGGTCACCATGGGCATGAAACAGAGCGTCGATACGTTAAGGACGGGTCTGGCTATGGGGGCTGATAGGGCTATACACGTGGAGACTAGTGGGCAGTTATACCCTTTATCGGTTGCTAAGCTTTTGAGAGCTTTAGTGGAGCTTGAGAAGCCTGGTCTTCTCATTCTGGGCAAACAGGCTATTGATGATGATTGCAATCAAACTGGGCAAATGGTGGCAGGGCTGTTGAACTGGCCTCAAGGCACTTTTGCTTCAAAGGTTGTGCTGGATAAGGAGAAGCAGGTAGTGACAGTGGACAGAGAGGTAGATGGGGGTCTTGAGACTCTCTGTCTAGATTTACCAGCAGTGATTACCACTGATTTGAGGCTAAATCAACCAAGGTATGCAACACTCCCCAACATAATGAAAGCAAAATCAAAGGTCATAAAGAAGTTCACTCCACAGGATCTGAATGTGGAAATCAAATCCGACATAGAAGAGGTTCAAGTCACAGAACCTCCCAAGAGAAAAGCAGGGGTTATCGTTTCTTCTGTGGATGAGCTGATTGACAAGCTGAAAAACGAAGCCCGTGTCATTTGA

Protein Analysis

294

Amino Acids

32.51

Weight (kDa)

8.85

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETF PF01012 69 - 251 2.5e-39 Electron transfer flavoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43430 AT5G43430 AT5G43430 AT5G43430
fragaria_vesca FvH4_4g03430 FvH4_4g03430
malus_domestica MD13G1212500.v1.1
prunus_persica Prupe.1G037500_v2.0.a1
pyrus_communis pycom13g18430
rosa_chinensis RchiOBHm_Chr4g0392411
rosa_laevigata RLG00000009813
rosa_multiflora Rmu_sc0001800.1_g000016
rosa_roxburghii Rroxscaffold_5G00337590
rosa_rugosa Rorug03G0338700 Rorug03G0338800
rosa_samantha Rh4AG042500 Rh4BG037400 Rh4CG045300 Rh4DG039800
rosa_wichuraiana Rw4G003320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 577
AccBSI CCGCTC 2 cut(s) 123, 201
AccI GTMKAC 1 cut(s) 162
AccII CGCG 1 cut(s) 156
AciI CCGC 3 cut(s) 121, 201, 308
AclWI GGATC 2 cut(s) 281, 759
AcvI CACGTG 1 cut(s) 385
AfiI CCNNNNNNNGG 1 cut(s) 415
AflIII ACRYGT 2 cut(s) 154, 382
AgsI TTSAA 4 cut(s) 79, 535, 561, 791
AhdI GACNNNNNGTC 1 cut(s) 633
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 5 cut(s) 427, 437, 448, 847, 859
AluI AGCT 5 cut(s) 427, 437, 448, 847, 859
Alw26I GTCTC 4 cut(s) 57, 203, 383, 620
AlwI GGATC 2 cut(s) 281, 759
AlwNI CAGNNNCTG 1 cut(s) 754
AoxI GGCC 3 cut(s) 264, 291, 539
ArsI GACNNNNNNTTYG 5 cut(s) 144, 168, 176, 200, 861
Asp700I GAANNNNTTC 1 cut(s) 786
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 2 cut(s) 47, 304
BbrPI CACGTG 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 455
BccI CCATC 1 cut(s) 608
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 4 cut(s) 57, 203, 383, 620
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 2 cut(s) 393, 638
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 1 cut(s) 202
BlpI GCTNAGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 203
Bme1390I CCNGG 1 cut(s) 459
BmeRI GACNNNNNGTC 1 cut(s) 633
BmgT120I GGNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 459
BmrI ACTGGG 1 cut(s) 519
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 455
Bpu1102I GCTNAGC 1 cut(s) 423
BpuEI CTTGAG 5 cut(s) 28, 109, 470, 528, 644
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 297, 315, 683
BsaXI ACNNNNNCTCC 2 cut(s) 787, 817
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 2 cut(s) 514, 542
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 297, 315, 683
BseGI GGATG 2 cut(s) 68, 847
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 1 cut(s) 415
BseNI ACTGG 2 cut(s) 514, 542
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 3 cut(s) 266, 293, 541
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 4 cut(s) 57, 203, 383, 620
BsnI GGCC 3 cut(s) 266, 293, 541
Bso31I GGTCTC 1 cut(s) 203
Bsp143I GATC 4 cut(s) 65, 249, 273, 751
Bsp1720I GCTNAGC 1 cut(s) 423
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 3 cut(s) 121, 201, 308
BspANI GGCC 3 cut(s) 266, 293, 541
BspFNI CGCG 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 2 cut(s) 281, 759
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 2 cut(s) 123, 201
BsrI ACTGG 2 cut(s) 514, 542
BssECI CCNNGG 3 cut(s) 297, 315, 683
BssMI GATC 4 cut(s) 65, 249, 273, 751
BssT1I CCWWGG 2 cut(s) 315, 683
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 598
Bst6I CTCTTC 3 cut(s) 27, 255, 777
BstBAI YACGTR 1 cut(s) 385
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 310
BstF5I GGATG 2 cut(s) 68, 847
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 4 cut(s) 68, 252, 276, 754
BstMAI GTCTC 4 cut(s) 57, 203, 383, 620
BstMBI GATC 4 cut(s) 65, 249, 273, 751
BstMWI GCNNNNNNNGC 3 cut(s) 365, 374, 454
BstNI CCWGG 1 cut(s) 459
BstPI GGTNACC 1 cut(s) 310
BstSCI CCNGG 1 cut(s) 457
BstUI CGCG 1 cut(s) 156
BstV2I GAAGAC 1 cut(s) 455
BstX2I RGATCY 1 cut(s) 751
BstYI RGATCY 1 cut(s) 751
BsuRI GGCC 3 cut(s) 266, 293, 541
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 68, 847
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 3 cut(s) 603, 657, 660
BveI ACCTGC 1 cut(s) 577
CaiI CAGNNNCTG 1 cut(s) 754
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 1 cut(s) 322
CviAII CATG 3 cut(s) 130, 316, 322
DdeI CTNAG 1 cut(s) 423
DpnI GATC 4 cut(s) 67, 251, 275, 753
DpnII GATC 4 cut(s) 65, 249, 273, 751
DrdI GACNNNNNNGTC 1 cut(s) 170
DriI GACNNNNNGTC 1 cut(s) 633
DseDI GACNNNNNNGTC 1 cut(s) 170
Eam1104I CTCTTC 3 cut(s) 27, 255, 777
Eam1105I GACNNNNNGTC 1 cut(s) 633
EarI CTCTTC 3 cut(s) 27, 255, 777
Eco130I CCWWGG 2 cut(s) 315, 683
Eco31I GGTCTC 1 cut(s) 203
Eco72I CACGTG 1 cut(s) 385
Eco91I GGTNACC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 457
EcoT14I CCWWGG 2 cut(s) 315, 683
ErhI CCWWGG 2 cut(s) 315, 683
FaeI CATG 3 cut(s) 133, 319, 325
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FatI CATG 3 cut(s) 129, 315, 321
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 2 cut(s) 55, 854
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 2 cut(s) 393, 638
GluI GCNGC 1 cut(s) 202
HaeIII GGCC 3 cut(s) 266, 293, 541
HgaI GACGC 1 cut(s) 322
Hin1II CATG 3 cut(s) 133, 319, 325
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 3 cut(s) 183, 281, 628
HphI GGTGA 2 cut(s) 47, 304
Hpy166II GTNNAC 3 cut(s) 163, 601, 741
Hpy188I TCNGA 6 cut(s) 182, 193, 273, 298, 756, 775
Hpy188III TCNNGA 2 cut(s) 623, 638
Hpy8I GTNNAC 3 cut(s) 163, 601, 741
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4IV ACGT 3 cut(s) 219, 341, 384
HpyCH4V TGCA 2 cut(s) 501, 692
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 374, 454
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 3 cut(s) 219, 341, 384
Hsp92II CATG 3 cut(s) 133, 319, 325
Kzo9I GATC 4 cut(s) 65, 249, 273, 751
LmnI GCTCC 1 cut(s) 445
MaeI CTAG 2 cut(s) 393, 638
MaeII ACGT 3 cut(s) 219, 341, 384
MaeIII GTNAC 4 cut(s) 6, 310, 592, 793
MalI GATC 4 cut(s) 67, 251, 275, 753
MbiI CCGCTC 2 cut(s) 123, 201
MboI GATC 4 cut(s) 65, 249, 273, 751
MboII GAAGA 8 cut(s) 14, 17, 145, 235, 272, 455, 794, 825
MflI RGATCY 1 cut(s) 751
MluI ACGCGT 1 cut(s) 154
MlyI GAGTC 3 cut(s) 192, 290, 622
MmeI TCCRAC 3 cut(s) 96, 216, 798
MnlI CCTC 8 cut(s) 256, 292, 304, 552, 601, 663, 778, 813
MroXI GAANNNNTTC 1 cut(s) 786
MseI TTAA 2 cut(s) 87, 344
MslI CAYNNNNRTG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 365, 374, 454
NcoI CCATGG 1 cut(s) 315
NdeII GATC 4 cut(s) 65, 249, 273, 751
NlaIII CATG 3 cut(s) 133, 319, 325
NmuCI GTSAC 3 cut(s) 310, 592, 793
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 786
PkrI GCNGC 1 cut(s) 203
PleI GAGTC 3 cut(s) 191, 289, 622
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 3 cut(s) 191, 289, 622
Ppu21I YACGTR 1 cut(s) 385
Psp6I CCWGG 1 cut(s) 457
PspCI CACGTG 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 310
PspGI CCWGG 1 cut(s) 457
PspPI GGNCC 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 754
PsuI RGATCY 1 cut(s) 751
RseI CAYNNNNRTG 1 cut(s) 320
SalI GTCGAC 1 cut(s) 161
SaqAI TTAA 2 cut(s) 87, 344
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 4 cut(s) 65, 249, 273, 751
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 3 cut(s) 192, 290, 622
ScrFI CCNGG 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 5 cut(s) 43, 124, 449, 543, 623
SmoI CTYRAG 5 cut(s) 43, 124, 449, 543, 623
SpeI ACTAGT 1 cut(s) 392
SsiI CCGC 3 cut(s) 121, 201, 308
SspMI CTAG 2 cut(s) 393, 638
StyD4I CCNGG 1 cut(s) 457
StyI CCWWGG 2 cut(s) 315, 683
TaaI ACNGT 1 cut(s) 598
TaiI ACGT 3 cut(s) 222, 344, 387
TaqI TCGA 3 cut(s) 162, 230, 336
TauI GCSGC 1 cut(s) 204
Tru1I TTAA 2 cut(s) 87, 344
Tru9I TTAA 2 cut(s) 87, 344
TscAI CASTG 3 cut(s) 603, 657, 667
TseFI GTSAC 3 cut(s) 310, 592, 793
Tsp45I GTSAC 3 cut(s) 310, 592, 793
TspDTI ATGAA 4 cut(s) 146, 248, 338, 725
TspRI CASTG 3 cut(s) 603, 657, 667
XbaI TCTAGA 1 cut(s) 637
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 2 cut(s) 393, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.