Rh4BG230200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016452
Physical Location & Seq
Reverse (-)
39496521 .. 39496880
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG230200.1

Sequence Viewer

Length: 360 bp
ATGGAGCAGAGGAGAGAGAACGATTTGCCGGATCCGATGGGAGACTCGAGCAAGGCATGGCAGTGGTCGTCCCAGACAGCTCGATCGAGAGTAGAGACGACTCTGCTCATGAATGCCTCCTGGGCTGCATACGGCTGGTACGGGAACTCGACGTCAATGCCTCCGATGCGGTATAATCTTTTCAATGAACTTTTAGGGTTTGTTCCCGGGAACTCGACATCGATGCATCCTCTACTGCCGGGTCCAAATCCATGCCCCAATCACCAACGCTGTTACTCCGCCGCCCAGAACCTTGCTCCCCAAAAACAAGATCAAATCTTGTTCCTCCGTCACCCTTCCCAGAACCTCTGGAATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.63

Weight (kDa)

7.84

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 155
AciI CCGC 3 cut(s) 169, 279, 282
AclWI GGATC 2 cut(s) 26, 39
AcsI RAATTY 1 cut(s) 352
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 1 cut(s) 140
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 1 cut(s) 119
AluBI AGCT 1 cut(s) 80
AluI AGCT 1 cut(s) 80
Alw26I GTCTC 2 cut(s) 36, 89
AlwI GGATC 2 cut(s) 26, 39
Ama87I CYCGRG 2 cut(s) 46, 206
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 1 cut(s) 352
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 3 cut(s) 207, 208, 240
AsuHPI GGTGA 2 cut(s) 254, 323
AvaI CYCGRG 2 cut(s) 46, 206
AvaII GGWCC 1 cut(s) 242
BamHI GGATCC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 112
BccI CCATC 1 cut(s) 31
BceAI ACGGC 1 cut(s) 148
BcgI CGANNNNNNTGC 4 cut(s) 139, 173, 205, 239
BciT130I CCWGG 1 cut(s) 121
BcnI CCSGG 3 cut(s) 207, 208, 240
BcoDI GTCTC 2 cut(s) 36, 89
BfaI CTAG 1 cut(s) 358
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 2 cut(s) 126, 282
BlsI GCNGC 2 cut(s) 127, 283
Bme1390I CCNGG 4 cut(s) 121, 207, 208, 240
Bme18I GGWCC 1 cut(s) 242
BmeT110I CYCGRG 2 cut(s) 46, 206
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 33, 243
BmrFI CCNGG 4 cut(s) 121, 207, 208, 240
BmsI GCATC 3 cut(s) 156, 213, 235
BpuMI CCSGG 3 cut(s) 207, 208, 240
Bsa29I ATCGAT 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 120, 206
BseBI CCWGG 1 cut(s) 121
BseCI ATCGAT 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 120, 206
BseGI GGATG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 112
Bsh1285I CGRYCG 1 cut(s) 86
BshVI ATCGAT 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 86
BsiHKCI CYCGRG 2 cut(s) 46, 206
BsiSI CCGG 3 cut(s) 29, 207, 239
BslFI GGGAC 1 cut(s) 55
BsmAI GTCTC 2 cut(s) 36, 89
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 55
BsmI GAATGC 1 cut(s) 118
BsoBI CYCGRG 2 cut(s) 46, 206
Bsp143I GATC 3 cut(s) 31, 83, 310
BspACI CCGC 3 cut(s) 169, 279, 282
BspDI ATCGAT 1 cut(s) 221
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 33, 243
BspPI GGATC 2 cut(s) 26, 39
BssECI CCNNGG 2 cut(s) 120, 206
BssMI GATC 3 cut(s) 31, 83, 310
BssNI GRCGYC 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 121
BstACI GRCGYC 1 cut(s) 152
BstF5I GGATG 1 cut(s) 226
BstKTI GATC 3 cut(s) 34, 86, 313
BstMAI GTCTC 2 cut(s) 36, 89
BstMBI GATC 3 cut(s) 31, 83, 310
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 2 cut(s) 122, 166
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 4 cut(s) 119, 205, 206, 238
BstV1I GCAGC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 221
BsuTUI ATCGAT 1 cut(s) 221
BtsCI GGATG 1 cut(s) 226
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 68
CciI TCATGA 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 242
Cfr9I CCCGGG 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 221
Csp6I GTAC 1 cut(s) 139
CviAII CATG 3 cut(s) 57, 109, 252
CviJI RGCY 3 cut(s) 80, 125, 135
CviKI_1 RGCY 3 cut(s) 80, 125, 135
CviQI GTAC 1 cut(s) 139
DpnI GATC 3 cut(s) 33, 85, 312
DpnII GATC 3 cut(s) 31, 83, 310
EciI GGCGGA 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 242
Eco88I CYCGRG 2 cut(s) 46, 206
EcoRII CCWGG 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 3 cut(s) 60, 112, 255
FaiI YATR 5 cut(s) 58, 110, 130, 174, 253
FaqI GGGAC 1 cut(s) 55
FatI CATG 3 cut(s) 56, 108, 251
Fnu4HI GCNGC 2 cut(s) 126, 282
FokI GGATG 1 cut(s) 213
Fsp4HI GCNGC 2 cut(s) 126, 282
FspBI CTAG 1 cut(s) 358
GluI GCNGC 2 cut(s) 126, 282
HapII CCGG 3 cut(s) 29, 207, 239
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 3 cut(s) 60, 112, 255
HinfI GANTC 2 cut(s) 44, 100
HpaII CCGG 3 cut(s) 29, 207, 239
HphI GGTGA 2 cut(s) 254, 323
Hpy188I TCNGA 2 cut(s) 36, 165
Hpy188III TCNNGA 3 cut(s) 87, 109, 349
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 1 cut(s) 345
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 128, 226
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 166
HpySE526I ACGT 1 cut(s) 152
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 3 cut(s) 60, 112, 255
Kzo9I GATC 3 cut(s) 31, 83, 310
LmnI GCTCC 2 cut(s) 4, 301
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 3 cut(s) 156, 213, 235
MaeI CTAG 1 cut(s) 358
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 2 cut(s) 272, 329
MalI GATC 3 cut(s) 33, 85, 312
MboI GATC 3 cut(s) 31, 83, 310
MflI RGATCY 1 cut(s) 31
MluCI AATT 1 cut(s) 352
MlyI GAGTC 2 cut(s) 38, 94
MnlI CCTC 6 cut(s) 3, 127, 171, 240, 335, 356
Mph1103I ATGCAT 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 61
MspI CCGG 3 cut(s) 29, 207, 239
MspR9I CCNGG 4 cut(s) 121, 207, 208, 240
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 2 cut(s) 122, 166
NciI CCSGG 3 cut(s) 207, 208, 240
NdeII GATC 3 cut(s) 31, 83, 310
NlaIII CATG 3 cut(s) 60, 112, 255
NlaIV GGNNCC 2 cut(s) 33, 243
NmuCI GTSAC 1 cut(s) 329
NsiI ATGCAT 1 cut(s) 228
PaeR7I CTCGAG 1 cut(s) 46
PagI TCATGA 1 cut(s) 108
PctI GAATGC 1 cut(s) 118
PkrI GCNGC 2 cut(s) 127, 283
Ple19I CGATCG 1 cut(s) 86
PleI GAGTC 2 cut(s) 38, 94
PpsI GAGTC 2 cut(s) 38, 94
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 33, 243
PspPI GGNCC 1 cut(s) 242
PspXI VCTCGAGB 1 cut(s) 46
PsuI RGATCY 1 cut(s) 31
PvuI CGATCG 1 cut(s) 86
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 61
SatI GCNGC 2 cut(s) 126, 282
Sau3AI GATC 3 cut(s) 31, 83, 310
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 2 cut(s) 38, 94
ScrFI CCNGG 4 cut(s) 121, 207, 208, 240
SetI ASST 4 cut(s) 82, 155, 294, 348
SfaNI GCATC 3 cut(s) 156, 213, 235
Sfr274I CTCGAG 1 cut(s) 46
SinI GGWCC 1 cut(s) 242
SlaI CTCGAG 1 cut(s) 46
SmaI CCCGGG 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 1 cut(s) 352
SsiI CCGC 3 cut(s) 169, 279, 282
SspMI CTAG 1 cut(s) 358
StyD4I CCNGG 4 cut(s) 119, 205, 206, 238
TaiI ACGT 1 cut(s) 155
TaqI TCGA 6 cut(s) 47, 82, 86, 149, 215, 221
TasI AATT 1 cut(s) 352
TauI GCSGC 1 cut(s) 284
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 1 cut(s) 125
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 2 cut(s) 125, 201
TspGWI ACGGA 1 cut(s) 317
TspMI CCCGGG 1 cut(s) 206
TspRI CASTG 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 46
XmaI CCCGGG 1 cut(s) 206
XspI CTAG 1 cut(s) 358
ZraI GACGTC 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.