Rh4CG049100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016452
Physical Location & Seq
Forward (+)
9770257 .. 9770553
297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG049100.1

Sequence Viewer

Length: 297 bp
ATGCAAAATCGAACTAACCATGGGATCGGTCATGGCCTTAGGGTTGGGCTTGGAATGGGGGAGATTGGCGTAGACGTTCTTGGCCTTGAAGCTTTGGTATCAAGCGAGAATGGAGCAGAGAAGAGAGAACGATTTGCCGGATCCGATGGGAGACTGGAGCAAGGCATGGCAGTGGTCGTCCTAGACAGCTCGATCGAGAGTGGAGACGACTTTGGTCATGAACGCCTCCTGGGCTGCGTACGGCTGGTACAGGAACTCAACGTCAATGCCTCCGATGCCGTACAAGCTTTTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

98

Amino Acids

10.42

Weight (kDa)

4.88

Isoelectric Point (pI)

24.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 72
AclWI GGATC 3 cut(s) 32, 135, 148
AfaI GTAC 3 cut(s) 240, 249, 282
AgsI TTSAA 1 cut(s) 89
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 3 cut(s) 92, 189, 287
AluI AGCT 3 cut(s) 92, 189, 287
Alw26I GTCTC 2 cut(s) 145, 198
AlwI GGATC 3 cut(s) 32, 135, 148
AoxI GGCC 2 cut(s) 34, 82
ApeKI GCWGC 1 cut(s) 234
AxyI CCTNAGG 1 cut(s) 38
BamHI GGATCC 1 cut(s) 140
BbvI GCAGC 1 cut(s) 221
BccI CCATC 1 cut(s) 140
BceAI ACGGC 2 cut(s) 257, 263
BciT130I CCWGG 1 cut(s) 230
BcoDI GTCTC 2 cut(s) 145, 198
BfaI CTAG 1 cut(s) 182
BglI GCCNNNNNGGC 1 cut(s) 231
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
Bme1390I CCNGG 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 265
BoxI GACNNNNGTC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 19, 229
Bse1I ACTGG 1 cut(s) 159
Bse21I CCTNAGG 1 cut(s) 38
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 19, 229
BseNI ACTGG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 2 cut(s) 36, 84
BsiEI CGRYCG 1 cut(s) 195
BsiSI CCGG 1 cut(s) 138
BsiWI CGTACG 1 cut(s) 238
BsmAI GTCTC 2 cut(s) 145, 198
BsmBI CGTCTC 1 cut(s) 198
BsnI GGCC 2 cut(s) 36, 84
Bsp143I GATC 3 cut(s) 24, 140, 192
Bsp19I CCATGG 1 cut(s) 19
BspANI GGCC 2 cut(s) 36, 84
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 142
BspPI GGATC 3 cut(s) 32, 135, 148
BsrI ACTGG 1 cut(s) 159
BssECI CCNNGG 2 cut(s) 19, 229
BssMI GATC 3 cut(s) 24, 140, 192
BssT1I CCWWGG 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 116
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 19
BstKTI GATC 3 cut(s) 27, 143, 195
BstMAI GTCTC 2 cut(s) 145, 198
BstMBI GATC 3 cut(s) 24, 140, 192
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 3 cut(s) 231, 275, 284
BstNI CCWGG 1 cut(s) 230
BstPAI GACNNNNGTC 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 38
BsuRI GGCC 2 cut(s) 36, 84
BtgI CCRYGG 1 cut(s) 19
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 177
CciI TCATGA 1 cut(s) 217
Csp6I GTAC 3 cut(s) 239, 248, 281
CviAII CATG 4 cut(s) 20, 32, 166, 218
CviJI RGCY 8 cut(s) 36, 49, 84, 92, 189, 234, 244, 287
CviKI_1 RGCY 8 cut(s) 36, 49, 84, 92, 189, 234, 244, 287
CviQI GTAC 3 cut(s) 239, 248, 281
DdeI CTNAG 1 cut(s) 38
DpnI GATC 3 cut(s) 26, 142, 194
DpnII GATC 3 cut(s) 24, 140, 192
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 19
Eco81I CCTNAGG 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 4 cut(s) 23, 35, 169, 221
FaiI YATR 4 cut(s) 21, 33, 167, 219
FatI CATG 4 cut(s) 19, 31, 165, 217
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 1 cut(s) 235
Fsp4HI GCNGC 1 cut(s) 235
FspBI CTAG 1 cut(s) 182
GluI GCNGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 2 cut(s) 36, 84
HapII CCGG 1 cut(s) 138
Hin1II CATG 4 cut(s) 23, 35, 169, 221
HindIII AAGCTT 2 cut(s) 90, 285
HpaII CCGG 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 145, 274
Hpy188III TCNNGA 2 cut(s) 196, 218
Hpy8I GTNNAC 1 cut(s) 73
HpyCH4IV ACGT 2 cut(s) 75, 261
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 275, 284
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 2 cut(s) 75, 261
Hsp92II CATG 4 cut(s) 23, 35, 169, 221
Kzo9I GATC 3 cut(s) 24, 140, 192
LmnI GCTCC 2 cut(s) 113, 157
LpnPI CCDG 6 cut(s) 140, 151, 215, 230, 236, 242
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 182
MaeII ACGT 2 cut(s) 75, 261
MalI GATC 3 cut(s) 26, 142, 194
MboI GATC 3 cut(s) 24, 140, 192
MboII GAAGA 1 cut(s) 133
MflI RGATCY 1 cut(s) 140
MnlI CCTC 2 cut(s) 236, 280
MslI CAYNNNNRTG 1 cut(s) 170
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 1 cut(s) 230
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 3 cut(s) 231, 275, 284
NcoI CCATGG 1 cut(s) 19
NdeII GATC 3 cut(s) 24, 140, 192
NlaIII CATG 4 cut(s) 23, 35, 169, 221
NlaIV GGNNCC 1 cut(s) 142
PagI TCATGA 1 cut(s) 217
Pfl23II CGTACG 1 cut(s) 238
PkrI GCNGC 1 cut(s) 236
Ple19I CGATCG 1 cut(s) 195
PshAI GACNNNNGTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspLI CGTACG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 142
PsuI RGATCY 1 cut(s) 140
PvuI CGATCG 1 cut(s) 195
RsaI GTAC 3 cut(s) 240, 249, 282
RsaNI GTAC 3 cut(s) 239, 248, 281
RseI CAYNNNNRTG 1 cut(s) 170
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 3 cut(s) 24, 140, 192
ScrFI CCNGG 1 cut(s) 230
SetI ASST 5 cut(s) 78, 94, 191, 264, 289
SfaNI GCATC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 170
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 1 cut(s) 19
TaiI ACGT 2 cut(s) 78, 264
TaqI TCGA 4 cut(s) 10, 191, 195, 292
TaqII GACCGA 1 cut(s) 17
TscAI CASTG 1 cut(s) 177
TseI GCWGC 1 cut(s) 234
TspDTI ATGAA 1 cut(s) 234
TspRI CASTG 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.