Rh4BG372800

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012626
Physical Location & Seq
Forward (+)
52968188 .. 52968724
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG372800.1

Sequence Viewer

Length: 537 bp
ATGAAGAAGAAGAAGAAGAAAACCAAAACTGAGCCAGCCAAGATTGACAGTGAAGATGTCCTTCTCCGACTCCCATACTACATAATCCTGGACATCTTTTGCAAAATCCCCACCAAAAAACTACTTCAGTTCAAAACTGTGTGCAAGTCTTGTCACTCTTTCCTCTCCGATCCTCAGTTCGCCAAAGACTTATCTTCTCAAACTCATCCTTGCTTTATGATGTCGAGAAGCCCCCCGAATGGCTTGTTTATAGTCGATCCTGAAAAGGCCTCGTGCCCGGACGGCCTCTTACTTAGGGTGCCTAAGCCTGCTAACGATGATCCATATTGGATACGTGATATCGTGGGTTCATGCAATGGCCTCCTCTGCATACGCCACTCTCGCTTCTTCAAGCATAAAAAACAGCCTAATTTTTTCGCTTTTTACCTTTTGCATCCCATTACTGGTGAGACTTTAGCTCTTCCGAGACTCAAGAACGTTCAAAAGAGTGGAGCCTTTATGTGCTTATGGGTTTCGTCCCATCACCCACGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

178

Amino Acids

20.45

Weight (kDa)

9.63

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 21 - 60 6.2e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 298
AclI AACGTT 1 cut(s) 477
AclWI GGATC 3 cut(s) 164, 251, 314
AcuI CTGAAG 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 239, 443
AgsI TTSAA 3 cut(s) 133, 391, 482
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 1 cut(s) 458
AluI AGCT 1 cut(s) 458
Alw26I GTCTC 2 cut(s) 443, 460
AlwI GGATC 3 cut(s) 164, 251, 314
AoxI GGCC 3 cut(s) 267, 283, 358
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 458, 515
BaeGI GKGCMC 1 cut(s) 278
BanI GGYRCC 1 cut(s) 298
BauI CACGAG 2 cut(s) 271, 528
BccI CCATC 1 cut(s) 528
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 1 cut(s) 89
BciVI GTATCC 1 cut(s) 324
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 2 cut(s) 443, 460
BfuI GTATCC 1 cut(s) 324
BglI GCCNNNNNGGC 1 cut(s) 282
Bme1390I CCNGG 2 cut(s) 89, 278
BmiI GGNNCC 2 cut(s) 300, 493
BmrFI CCNGG 2 cut(s) 89, 278
BmsI GCATC 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 303
BpuEI CTTGAG 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 335
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 443
Bse1I ACTGG 1 cut(s) 448
Bse3DI GCAATG 1 cut(s) 361
BseBI CCWGG 1 cut(s) 89
BseGI GGATG 2 cut(s) 205, 433
BseLI CCNNNNNNNGG 2 cut(s) 239, 443
BseMI GCAATG 1 cut(s) 361
BseMII CTCAG 2 cut(s) 21, 188
BseNI ACTGG 1 cut(s) 448
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 1 cut(s) 278
BshFI GGCC 3 cut(s) 269, 285, 360
BshNI GGYRCC 1 cut(s) 298
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 1 cut(s) 502
BslI CCNNNNNNNGG 2 cut(s) 239, 443
BsmAI GTCTC 2 cut(s) 443, 460
BsmFI GGGAC 1 cut(s) 502
BsnI GGCC 3 cut(s) 269, 285, 360
Bsp1286I GDGCHC 1 cut(s) 278
Bsp143I GATC 3 cut(s) 169, 256, 319
BspANI GGCC 3 cut(s) 269, 285, 360
BspCNI CTCAG 2 cut(s) 22, 187
BspLI GGNNCC 2 cut(s) 300, 493
BspPI GGATC 3 cut(s) 164, 251, 314
BspQI GCTCTTC 1 cut(s) 465
BspT107I GGYRCC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 361
BsrI ACTGG 1 cut(s) 448
BssMI GATC 3 cut(s) 169, 256, 319
BssSI CACGAG 2 cut(s) 271, 528
Bst2BI CACGAG 2 cut(s) 271, 528
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 2 cut(s) 50, 139
Bst6I CTCTTC 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 36, 309
BstDEI CTNAG 4 cut(s) 30, 174, 293, 303
BstF5I GGATG 2 cut(s) 205, 433
BstKTI GATC 3 cut(s) 172, 259, 322
BstMAI GTCTC 2 cut(s) 443, 460
BstMBI GATC 3 cut(s) 169, 256, 319
BstMWI GCNNNNNNNGC 3 cut(s) 282, 366, 381
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 2 cut(s) 87, 276
BstSLI GKGCMC 1 cut(s) 278
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 3 cut(s) 269, 285, 360
BtsCI GGATG 2 cut(s) 205, 433
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 36, 309
CviAII CATG 1 cut(s) 351
DdeI CTNAG 4 cut(s) 30, 174, 293, 303
DpnI GATC 3 cut(s) 171, 258, 321
DpnII GATC 3 cut(s) 169, 256, 319
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Eco147I AGGCCT 1 cut(s) 269
Eco32I GATATC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 87
EcoRV GATATC 1 cut(s) 340
FaeI CATG 1 cut(s) 354
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 1 cut(s) 502
FatI CATG 1 cut(s) 350
FokI GGATG 2 cut(s) 192, 420
HaeIII GGCC 3 cut(s) 269, 285, 360
HapII CCGG 1 cut(s) 278
Hin1II CATG 1 cut(s) 354
HinfI GANTC 2 cut(s) 69, 468
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 458, 515
Hpy188I TCNGA 3 cut(s) 68, 169, 465
Hpy188III TCNNGA 3 cut(s) 225, 260, 472
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 2 cut(s) 50, 139
HpyCH4IV ACGT 2 cut(s) 334, 477
HpyCH4V TGCA 5 cut(s) 102, 144, 354, 369, 433
HpyF10VI GCNNNNNNNGC 3 cut(s) 282, 366, 381
HpyF3I CTNAG 4 cut(s) 30, 174, 293, 303
HpySE526I ACGT 2 cut(s) 334, 477
Hsp92II CATG 1 cut(s) 354
Kzo9I GATC 3 cut(s) 169, 256, 319
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 1 cut(s) 491
LpnPI CCDG 7 cut(s) 48, 74, 101, 273, 291, 321, 429
LweI GCATC 1 cut(s) 442
MaeII ACGT 2 cut(s) 334, 477
MaeIII GTNAC 1 cut(s) 152
MalI GATC 3 cut(s) 171, 258, 321
MboI GATC 3 cut(s) 169, 256, 319
MboII GAAGA 9 cut(s) 16, 19, 22, 25, 28, 65, 186, 379, 452
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 1 cut(s) 409
MlyI GAGTC 2 cut(s) 63, 462
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 6 cut(s) 173, 183, 280, 296, 371, 374
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 2 cut(s) 89, 278
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 3 cut(s) 282, 366, 381
NciI CCSGG 1 cut(s) 278
NdeII GATC 3 cut(s) 169, 256, 319
NlaIII CATG 1 cut(s) 354
NlaIV GGNNCC 2 cut(s) 300, 493
NmuCI GTSAC 1 cut(s) 152
PceI AGGCCT 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 465
PfoI TCCNGGA 1 cut(s) 87
PleI GAGTC 2 cut(s) 63, 462
PpsI GAGTC 2 cut(s) 63, 462
Ppu21I YACGTR 1 cut(s) 335
Psp1406I AACGTT 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 300, 493
SapI GCTCTTC 1 cut(s) 465
Sau3AI GATC 3 cut(s) 169, 256, 319
SchI GAGTC 2 cut(s) 63, 462
ScrFI CCNGG 2 cut(s) 89, 278
SduI GDGCHC 1 cut(s) 278
SetI ASST 4 cut(s) 337, 429, 460, 480
SfaNI GCATC 1 cut(s) 442
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 1 cut(s) 409
SseBI AGGCCT 1 cut(s) 269
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 87, 276
TaaI ACNGT 2 cut(s) 50, 139
TaiI ACGT 2 cut(s) 337, 480
TaqI TCGA 2 cut(s) 224, 255
TasI AATT 1 cut(s) 409
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 17, 339
TspRI CASTG 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.