Rh5BG383000

Protein SENSITIVE TO PROTON RHIZOTOXICITY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016106
Physical Location & Seq
Reverse (-)
60828089 .. 60829279
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG383000.1

Sequence Viewer

Length: 1191 bp
ATGAATATTAATCCGGGGGCCACATCGAATTGTTTCCAAGGTATGTACTCCATGACGGGTCAAGATACAGTTTCGTCGTTTTCACTCGAACCAGACTCATCATCATCTGGAGCAGTACTACCCCAAGATCAGAATCACTCGACTTCTCTTCTCTACAGCCTCTCCGTCCTCAAAGAGAAAGTTCGTCAAGCCAAATCGCTGGTCGGCATCCTTATTGCTCCTCCGGATCATCACCAAAGTCAACAACAACAACAACTTCCAGAGTCAACGACCATGGGGGCCGTGGCGAGCATGAACAATGTTATCCAAGAAATCATCGTCACCGCATCTGCGATGATGTTCACTTGCCAGCAGATGGCCCTTGTTGCTCCAGGTAACGACACAAGATCTGAACCAAACTTTAGTATTAGTACTGCTGGTCGTAGTAACATTAGAGACGCTGCTTTCTATTCAACAAATGATCAAACACTCGATTGGTTTAGTACTGAGAACTACAACAACTGTCCCACTAGGGATGTGATCAGTAGGAGTCATGGACAGGTCGAAACGAAAGAGTTTCCTCAAAGAGTTGAAACCAACAATGCGTTACAGGTACAAAATCTTCCCCCAAAGAACTCGAGCTACGACATAATTGAGTTGGATGCTGCTGATTTGTTGGCCAAGTACACTCATTACTGCCAAGTTTGCGGGAAAGGGTTCAAGCGTGATGCAAACTTGAGAATGCACATGAGGGCTCATGGGGATGAATACAAGACTAGCATTGCCTTGAGCAACCCTTTGAAGAACAACAATAAGCAGAATAGTGCAGTGAGCATACATGGATCGAGCGATGCTGAGTGTTCGGTGAAATTACCTAGGAAATATTCATGTCCTCAAGAAGGGTGCAGGTGGAATCAAAAGCACGCCAAATTCCAGCCTCTGAAGTCCATGATCTGCGTGAAAAATCACTACAAGAGGAGCCATTGTCCGAAGATGTATGTATGTAAGAGGTGCAATCGGAAGCAGTTTTCTGTGTTATCTGATCTAAGAACGCATGAAAAGCACTGTGGGGATCTCAGGTGGCAATGCTCTTGCGGAACCACATTTTCTAGGAAAGATAAGCTTATGGGTCATGTTGCTTTGTTCGTTGGACACACACCAGTTATACTCGGTTCTAATTTGCCTATTAGGCATGGAAAAGCTGTGAACTAA

Protein Analysis

396

Amino Acids

44.15

Weight (kDa)

9.01

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_STOP2_3rd PF23115 288 - 322 4.3e-22 STOP2-like, C2H2-type zinc finger
C2CH-3rd_BIRD-IDD PF22995 326 - 350 6.2e-06 BIRD-IDD transcription factor, third C2HC zinc finger
zf-C2H2_STOP2_C PF23118 353 - 382 3.3e-17 STOP2-like, C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22890
fragaria_vesca FvH4_3g30580
malus_domestica MD03G1128200.v1.1 MD11G1149000.v1.1
prunus_persica Prupe.6G113300_v2.0.a1
pyrus_communis pycom11g12190
rosa_chinensis RchiOBHm_Chr5g0056621
rosa_laevigata RLG00000035112
rosa_multiflora Rmu_sc0004101.1_g000040 Rmu_sc0008982.1_g000004
rosa_rugosa Rorug05G0301000
rosa_samantha Rh5BG383000 Rh5CG406200 Rh5DG395800
rosa_wichuraiana Rw5G034790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 876
Acc36I ACCTGC 1 cut(s) 876
AccB7I CCANNNNNTGG 1 cut(s) 355
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 3 cut(s) 324, 687, 1074
AclWI GGATC 3 cut(s) 234, 829, 1059
AcoI YGGCCR 1 cut(s) 657
AcsI RAATTY 1 cut(s) 908
AcuI CTGAAG 1 cut(s) 941
AfaI GTAC 6 cut(s) 47, 117, 412, 484, 594, 665
AfiI CCNNNNNNNGG 2 cut(s) 355, 878
AgsI TTSAA 4 cut(s) 453, 572, 700, 781
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 3 cut(s) 621, 1102, 1181
AluI AGCT 3 cut(s) 621, 1102, 1181
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 3 cut(s) 234, 829, 1059
AlwNI CAGNNNCTG 1 cut(s) 919
Ama87I CYCGRG 1 cut(s) 616
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 4 cut(s) 18, 279, 357, 657
ApeKI GCWGC 2 cut(s) 440, 644
ApoI RAATTY 1 cut(s) 908
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AseI ATTAAT 1 cut(s) 9
Asp700I GAANNNNTTC 2 cut(s) 32, 695
AspA2I CCTAGG 1 cut(s) 854
AspS9I GGNCC 3 cut(s) 18, 279, 358
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 3 cut(s) 224, 313, 856
AvaI CYCGRG 1 cut(s) 616
AvrII CCTAGG 1 cut(s) 854
BalI TGGCCA 1 cut(s) 659
BanII GRGCYC 1 cut(s) 736
BarI GAAGNNNNNNTAC 2 cut(s) 585, 617
BbvI GCAGC 2 cut(s) 427, 631
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 266
BciT130I CCWGG 1 cut(s) 372
BclI TGATCA 2 cut(s) 460, 519
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 4 cut(s) 510, 756, 855, 1089
BfmI CTRYAG 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 876
BglI GCCNNNNNGGC 1 cut(s) 1168
BglII AGATCT 1 cut(s) 386
BisI GCNGC 2 cut(s) 441, 645
BlnI CCTAGG 1 cut(s) 854
BlsI GCNGC 2 cut(s) 442, 646
BmcAI AGTACT 3 cut(s) 117, 412, 484
Bme1390I CCNGG 2 cut(s) 15, 372
BmeT110I CYCGRG 1 cut(s) 616
BmgT120I GGNCC 3 cut(s) 18, 279, 358
BmiI GGNNCC 4 cut(s) 19, 280, 959, 1078
BmrFI CCNGG 2 cut(s) 15, 372
BmsI GCATC 5 cut(s) 216, 335, 631, 697, 820
BpmI CTGGAG 2 cut(s) 129, 354
BpuEI CTTGAG 3 cut(s) 736, 787, 858
BpuMI CCSGG 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 5 cut(s) 14, 37, 273, 282, 854
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 949, 979
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 878
Bse1I ACTGG 1 cut(s) 1139
Bse3DI GCAATG 2 cut(s) 759, 1070
Bse8I GATNNNNATC 1 cut(s) 132
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 5 cut(s) 14, 37, 273, 282, 854
BseGI GGATG 4 cut(s) 207, 520, 646, 748
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 355, 878
BseMI GCAATG 2 cut(s) 759, 1070
BseMII CTCAG 3 cut(s) 477, 825, 1069
BseNI ACTGG 1 cut(s) 1139
BseRI GAGGAG 2 cut(s) 210, 970
BseXI GCAGC 2 cut(s) 427, 631
BsgI GTGCAG 2 cut(s) 825, 904
BshFI GGCC 4 cut(s) 20, 281, 359, 659
BsiHKCI CYCGRG 1 cut(s) 616
BsiSI CCGG 2 cut(s) 14, 224
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 2 cut(s) 355, 878
BsmAI GTCTC 1 cut(s) 429
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 489
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 4 cut(s) 20, 281, 359, 659
BsoBI CYCGRG 1 cut(s) 616
Bsp1286I GDGCHC 1 cut(s) 736
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 3 cut(s) 324, 687, 1074
BspANI GGCC 4 cut(s) 20, 281, 359, 659
BspCNI CTCAG 3 cut(s) 478, 826, 1068
BspEI TCCGGA 1 cut(s) 223
BspLI GGNNCC 4 cut(s) 19, 280, 959, 1078
BspMI ACCTGC 1 cut(s) 876
BspPI GGATC 3 cut(s) 234, 829, 1059
BsrDI GCAATG 2 cut(s) 759, 1070
BsrI ACTGG 1 cut(s) 1139
BssECI CCNNGG 5 cut(s) 14, 37, 273, 282, 854
BssMI GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
BssT1I CCWWGG 3 cut(s) 37, 273, 854
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 3 cut(s) 70, 503, 1046
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 3 cut(s) 289, 350, 903
BstDEI CTNAG 4 cut(s) 486, 834, 1025, 1055
BstDSI CCRYGG 2 cut(s) 273, 282
BstENI CCTNNNNNAGG 1 cut(s) 876
BstF5I GGATG 4 cut(s) 207, 520, 646, 748
BstKTI GATC 9 cut(s) 130, 229, 389, 463, 522, 824, 933, 1024, 1054
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
BstMWI GCNNNNNNNGC 4 cut(s) 365, 684, 1039, 1168
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 13, 370
BstSFI CTRYAG 1 cut(s) 154
BstV1I GCAGC 2 cut(s) 427, 631
BstX2I RGATCY 2 cut(s) 386, 1051
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 2 cut(s) 386, 1051
BsuRI GGCC 4 cut(s) 20, 281, 359, 659
BtgI CCRYGG 2 cut(s) 273, 282
BtgZI GCGATG 2 cut(s) 347, 843
BtsCI GGATG 4 cut(s) 207, 520, 646, 748
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 2 cut(s) 813, 1042
BveI ACCTGC 1 cut(s) 876
Cac8I GCNNGC 3 cut(s) 289, 350, 903
CaiI CAGNNNCTG 1 cut(s) 919
Cfr13I GGNCC 3 cut(s) 18, 279, 358
CseI GACGC 1 cut(s) 446
Csp6I GTAC 6 cut(s) 46, 116, 411, 483, 593, 664
CviQI GTAC 6 cut(s) 46, 116, 411, 483, 593, 664
DdeI CTNAG 4 cut(s) 486, 834, 1025, 1055
DpnI GATC 9 cut(s) 129, 228, 388, 462, 521, 823, 932, 1023, 1053
DpnII GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco130I CCWWGG 3 cut(s) 37, 273, 854
Eco24I GRGCYC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 941
Eco88I CYCGRG 1 cut(s) 616
EcoNI CCTNNNNNAGG 1 cut(s) 876
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 3 cut(s) 37, 273, 854
EcoT38I GRGCYC 1 cut(s) 736
ErhI CCWWGG 3 cut(s) 37, 273, 854
Esp3I CGTCTC 1 cut(s) 429
FalI AAGNNNNNCTT 2 cut(s) 1086, 1118
FaqI GGGAC 1 cut(s) 489
FauI CCCGC 1 cut(s) 680
FbaI TGATCA 2 cut(s) 460, 519
Fnu4HI GCNGC 2 cut(s) 441, 645
FokI GGATG 4 cut(s) 194, 527, 653, 755
FriOI GRGCYC 1 cut(s) 736
Fsp4HI GCNGC 2 cut(s) 441, 645
FspBI CTAG 4 cut(s) 510, 756, 855, 1089
GluI GCNGC 2 cut(s) 441, 645
GsuI CTGGAG 2 cut(s) 129, 354
HaeIII GGCC 4 cut(s) 20, 281, 359, 659
HapII CCGG 2 cut(s) 14, 224
HgaI GACGC 1 cut(s) 446
HincII GTYRAC 2 cut(s) 242, 267
HindII GTYRAC 2 cut(s) 242, 267
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 5 cut(s) 95, 133, 263, 529, 892
HpaII CCGG 2 cut(s) 14, 224
HphI GGTGA 3 cut(s) 224, 313, 856
Hpy166II GTNNAC 5 cut(s) 242, 267, 342, 666, 1186
Hpy188I TCNGA 6 cut(s) 132, 391, 921, 969, 999, 1021
Hpy188III TCNNGA 5 cut(s) 62, 108, 224, 260, 875
Hpy8I GTNNAC 5 cut(s) 242, 267, 342, 666, 1186
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 872
HpyCH4III ACNGT 3 cut(s) 70, 503, 1046
HpyCH4V TGCA 5 cut(s) 710, 724, 806, 885, 993
HpyF10VI GCNNNNNNNGC 4 cut(s) 365, 684, 1039, 1168
HpyF3I CTNAG 4 cut(s) 486, 834, 1025, 1055
Kpn2I TCCGGA 1 cut(s) 223
Ksp22I TGATCA 2 cut(s) 460, 519
Kzo9I GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
LmnI GCTCC 4 cut(s) 110, 223, 373, 957
Lsp1109I GCAGC 2 cut(s) 427, 631
LweI GCATC 5 cut(s) 216, 335, 631, 697, 820
MaeI CTAG 4 cut(s) 510, 756, 855, 1089
MaeIII GTNAC 4 cut(s) 319, 374, 425, 585
MalI GATC 9 cut(s) 129, 228, 388, 462, 521, 823, 932, 1023, 1053
MboI GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
MboII GAAGA 4 cut(s) 140, 593, 793, 982
MflI RGATCY 2 cut(s) 386, 1051
MhlI GDGCHC 1 cut(s) 736
MlsI TGGCCA 1 cut(s) 659
MluCI AATT 5 cut(s) 28, 630, 848, 908, 1156
MluNI TGGCCA 1 cut(s) 659
MlyI GAGTC 3 cut(s) 89, 272, 538
MmeI TCCRAC 2 cut(s) 618, 1108
MnlI CCTC 9 cut(s) 170, 179, 231, 570, 723, 882, 927, 948, 981
Mox20I TGGCCA 1 cut(s) 659
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 2 cut(s) 32, 695
MscI TGGCCA 1 cut(s) 659
MseI TTAA 1 cut(s) 9
MslI CAYNNNNRTG 1 cut(s) 741
Msp20I TGGCCA 1 cut(s) 659
MspI CCGG 2 cut(s) 14, 224
MspR9I CCNGG 2 cut(s) 15, 372
Mva1269I GAATGC 1 cut(s) 726
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 4 cut(s) 365, 684, 1039, 1168
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 273
NdeII GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
NlaIV GGNNCC 4 cut(s) 19, 280, 959, 1078
NmuCI GTSAC 1 cut(s) 319
PaeR7I CTCGAG 1 cut(s) 616
PaqCI CACCTGC 1 cut(s) 876
PctI GAATGC 1 cut(s) 726
PdmI GAANNNNTTC 2 cut(s) 32, 695
PfeI GAWTC 2 cut(s) 133, 892
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 2 cut(s) 442, 646
PleI GAGTC 3 cut(s) 89, 271, 537
PpsI GAGTC 3 cut(s) 89, 271, 537
PshBI ATTAAT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 4 cut(s) 19, 280, 959, 1078
PspPI GGNCC 3 cut(s) 18, 279, 358
PspXI VCTCGAGB 1 cut(s) 616
PsrI GAACNNNNNNTAC 2 cut(s) 605, 637
PstNI CAGNNNCTG 1 cut(s) 919
PsuI RGATCY 2 cut(s) 386, 1051
RsaI GTAC 6 cut(s) 47, 117, 412, 484, 594, 665
RsaNI GTAC 6 cut(s) 46, 116, 411, 483, 593, 664
RseI CAYNNNNRTG 1 cut(s) 741
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 2 cut(s) 441, 645
Sau3AI GATC 9 cut(s) 127, 226, 386, 460, 519, 821, 930, 1021, 1051
Sau96I GGNCC 3 cut(s) 18, 279, 358
ScaI AGTACT 3 cut(s) 117, 412, 484
SchI GAGTC 3 cut(s) 89, 272, 538
ScrFI CCNGG 2 cut(s) 15, 372
SduI GDGCHC 1 cut(s) 736
SfaNI GCATC 5 cut(s) 216, 335, 631, 697, 820
SfcI CTRYAG 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 616
SlaI CTCGAG 1 cut(s) 616
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 4 cut(s) 616, 715, 766, 873
SmoI CTYRAG 4 cut(s) 616, 715, 766, 873
Sse9I AATT 5 cut(s) 28, 630, 848, 908, 1156
SsiI CCGC 3 cut(s) 324, 687, 1074
SspI AATATT 2 cut(s) 7, 863
SspMI CTAG 4 cut(s) 510, 756, 855, 1089
StyD4I CCNGG 2 cut(s) 13, 370
StyI CCWWGG 3 cut(s) 37, 273, 854
TaaI ACNGT 3 cut(s) 70, 503, 1046
TaqI TCGA 7 cut(s) 26, 87, 140, 471, 543, 617, 824
TasI AATT 5 cut(s) 28, 630, 848, 908, 1156
TatI WGTACW 5 cut(s) 45, 115, 410, 482, 663
TfiI GAWTC 2 cut(s) 133, 892
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 2 cut(s) 813, 1049
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 2 cut(s) 440, 644
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 5 cut(s) 17, 308, 759, 855, 1050
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 2 cut(s) 813, 1049
Van91I CCANNNNNTGG 1 cut(s) 355
VspI ATTAAT 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 876
XapI RAATTY 1 cut(s) 908
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 616
XmaJI CCTAGG 1 cut(s) 854
XmnI GAANNNNTTC 2 cut(s) 32, 695
XspI CTAG 4 cut(s) 510, 756, 855, 1089
ZrmI AGTACT 3 cut(s) 117, 412, 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.