Rh5CG222600

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013875
Physical Location & Seq
Reverse (-)
22670803 .. 22672191
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG222600.1

Sequence Viewer

Length: 828 bp
ATGGGAAAAAATGCTTTGGTTCTAGCATTTTACATAACCATATGCTGTATTGCTTTTGTAATATCGAAGATTATAATCGCAGTCCTCATCTATAGGCGGTGGAAGAGAAAGCACTTGGTTTTCGAACATGGCTTCTCAGGTGGGAAAATGGTTTTGTTCAGGGCACCAGTAATGCAATCTCTGAAATCAGATGTGTTCTTAAAGAAGACGCTGAAGTTAAGCAACAAGGACATCATTGGCTCAGGAGGGTATGGGACAGTTTATAAACTTACAATAAATGAAACCATGGCCTTTGCTGTGAAAAGACTGAACAGAGGAAGTGCAGATAGAGATCGAGGATTTGAGAGAGAACTCGAAGCAATGGGGGACATAAAGCACCGGAACATTGTGACTCTTCATGGATATTATACTGCTCCACATTATAACCTTCTTATATACGAACTGGTAGCTAATGGCAGCTTGGATGGAGTACTTTATGGAAGATCAATGACCAACAAGCGTTTAGATTGGCCGTCCAGATACAAAATAGCACTAGCTGGAACTTTTGGATACTTAGCTCCTGAATATTTTGACACCGGAAGAGCAACAGCAAAAGGGGATGTTTACAGTTTTGGAGTGGTTTTACTAGAGCTTCTAACTGGTAAAAAGCCTACAGATGAAGCATTTGTTGAGGAAGGAACAAAGCTTGTGACGTGGGTAAGTTATGAAAGAATATCAGAGATTGACCATGGAGAAGCTAACAACTATCAGAGATTTTGGGTTTTATTAAGAGCAAAAGAGTATTATCTTTCAGAACCAACTGTTTCTGTTACTGAAGGCCAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

275

Amino Acids

31.18

Weight (kDa)

9.34

Isoelectric Point (pI)

24.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 75 - 159 1.2e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 76 - 165 8.8e-13 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 178 - 220 4.8e-07 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 183 - 225 1.1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78530 AT1G78530
fragaria_vesca FvH4_3g17360
malus_domestica MD11G1273500.v1.1
prunus_persica Prupe.4G153600_v2.0.a1
pyrus_communis pycom11g24120
rosa_chinensis RchiOBHm_Chr5g0028881
rosa_laevigata RLG00000033122
rosa_multiflora Rmu_co8260251.1_g000001 Rmu_sc0000638.1_g000038
rosa_roxburghii Rroxscaffold_1G00051000
rosa_rugosa Rorug05G0111200
rosa_samantha Rh5AG203500 Rh5BG201600 Rh5CG222600 Rh5DG205200
rosa_wichuraiana Rw5G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 74, 264, 423
AccB1I GGYRCC 1 cut(s) 163
AciI CCGC 1 cut(s) 97
AcoI YGGCCR 1 cut(s) 509
AcuI CTGAAG 1 cut(s) 233
AfaI GTAC 1 cut(s) 471
AjiI CACGTC 1 cut(s) 693
AluBI AGCT 7 cut(s) 449, 459, 536, 557, 631, 685, 737
AluI AGCT 7 cut(s) 449, 459, 536, 557, 631, 685, 737
AoxI GGCC 3 cut(s) 288, 509, 817
ApeKI GCWGC 1 cut(s) 456
AsuII TTCGAA 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 468
BccI CCATC 1 cut(s) 458
BceAI ACGGC 1 cut(s) 496
BciVI GTATCC 1 cut(s) 542
BfaI CTAG 3 cut(s) 23, 533, 626
BfmI CTRYAG 2 cut(s) 91, 651
BfuI GTATCC 1 cut(s) 542
BisI GCNGC 1 cut(s) 457
BlsI GCNGC 1 cut(s) 458
BmcAI AGTACT 1 cut(s) 471
BmgBI CACGTC 1 cut(s) 693
BmiI GGNNCC 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 212
Bpu10I CCTNAGC 1 cut(s) 241
Bpu14I TTCGAA 1 cut(s) 123
BsaBI GATNNNNATC 2 cut(s) 74, 330
BsaJI CCNNGG 2 cut(s) 285, 727
BsaWI WCCGGW 2 cut(s) 378, 575
Bse1I ACTGG 3 cut(s) 167, 447, 643
Bse3DI GCAATG 1 cut(s) 366
Bse8I GATNNNNATC 2 cut(s) 74, 330
BseDI CCNNGG 2 cut(s) 285, 727
BseGI GGATG 2 cut(s) 469, 604
BseJI GATNNNNATC 2 cut(s) 74, 330
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 150, 255
BseNI ACTGG 3 cut(s) 167, 447, 643
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 1 cut(s) 468
BsgI GTGCAG 1 cut(s) 342
BshFI GGCC 3 cut(s) 290, 511, 819
BshNI GGYRCC 1 cut(s) 163
BsiSI CCGG 2 cut(s) 379, 576
BslFI GGGAC 2 cut(s) 268, 380
BsmFI GGGAC 2 cut(s) 268, 380
BsnI GGCC 3 cut(s) 290, 511, 819
Bsp119I TTCGAA 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 331, 482
Bsp19I CCATGG 2 cut(s) 285, 727
BspACI CCGC 1 cut(s) 97
BspANI GGCC 3 cut(s) 290, 511, 819
BspCNI CTCAG 2 cut(s) 149, 254
BspLI GGNNCC 1 cut(s) 165
BspQI GCTCTTC 1 cut(s) 574
BspT104I TTCGAA 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 3 cut(s) 167, 447, 643
BssECI CCNNGG 2 cut(s) 285, 727
BssMI GATC 2 cut(s) 331, 482
BssT1I CCWWGG 2 cut(s) 285, 727
Bst4CI ACNGT 3 cut(s) 259, 608, 802
Bst6I CTCTTC 3 cut(s) 98, 399, 574
BstBI TTCGAA 1 cut(s) 123
BstDEI CTNAG 3 cut(s) 136, 241, 553
BstDSI CCRYGG 2 cut(s) 285, 727
BstF5I GGATG 2 cut(s) 469, 604
BstKTI GATC 2 cut(s) 334, 485
BstMBI GATC 2 cut(s) 331, 482
BstSFI CTRYAG 2 cut(s) 91, 651
BstSLI GKGCMC 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 468
BstV2I GAAGAC 1 cut(s) 212
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 3 cut(s) 290, 511, 819
BtgI CCRYGG 2 cut(s) 285, 727
BtrI CACGTC 1 cut(s) 693
BtsCI GGATG 2 cut(s) 469, 604
CseI GACGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 470
CviAII CATG 4 cut(s) 128, 286, 398, 728
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 3 cut(s) 136, 241, 553
DpnI GATC 2 cut(s) 333, 484
DpnII GATC 2 cut(s) 331, 482
EaeI YGGCCR 1 cut(s) 509
Eam1104I CTCTTC 3 cut(s) 98, 399, 574
EarI CTCTTC 3 cut(s) 98, 399, 574
Eco130I CCWWGG 2 cut(s) 285, 727
Eco57I CTGAAG 1 cut(s) 233
EcoT14I CCWWGG 2 cut(s) 285, 727
ErhI CCWWGG 2 cut(s) 285, 727
FaeI CATG 4 cut(s) 131, 289, 401, 731
FaqI GGGAC 2 cut(s) 268, 380
FatI CATG 4 cut(s) 127, 285, 397, 727
FauNDI CATATG 1 cut(s) 41
Fnu4HI GCNGC 1 cut(s) 457
FokI GGATG 2 cut(s) 476, 611
Fsp4HI GCNGC 1 cut(s) 457
FspBI CTAG 3 cut(s) 23, 533, 626
GluI GCNGC 1 cut(s) 457
HaeIII GGCC 3 cut(s) 290, 511, 819
HapII CCGG 2 cut(s) 379, 576
HgaI GACGC 1 cut(s) 217
Hin1II CATG 4 cut(s) 131, 289, 401, 731
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 1 cut(s) 391
HpaII CCGG 2 cut(s) 379, 576
Hpy166II GTNNAC 1 cut(s) 604
Hpy188I TCNGA 5 cut(s) 183, 190, 718, 750, 793
Hpy188III TCNNGA 3 cut(s) 243, 516, 560
Hpy8I GTNNAC 1 cut(s) 604
HpyAV CCTTC 3 cut(s) 437, 668, 809
HpyCH4III ACNGT 3 cut(s) 259, 608, 802
HpyCH4IV ACGT 1 cut(s) 692
HpyCH4V TGCA 2 cut(s) 175, 323
HpyF3I CTNAG 3 cut(s) 136, 241, 553
HpySE526I ACGT 1 cut(s) 692
Hsp92II CATG 4 cut(s) 131, 289, 401, 731
Kzo9I GATC 2 cut(s) 331, 482
LguI GCTCTTC 1 cut(s) 574
LmnI GCTCC 2 cut(s) 418, 562
Lsp1109I GCAGC 1 cut(s) 468
MaeI CTAG 3 cut(s) 23, 533, 626
MaeII ACGT 1 cut(s) 692
MaeIII GTNAC 3 cut(s) 388, 688, 808
MalI GATC 2 cut(s) 333, 484
MboI GATC 2 cut(s) 331, 482
MboII GAAGA 6 cut(s) 79, 115, 217, 386, 492, 591
MhlI GDGCHC 1 cut(s) 166
MlyI GAGTC 1 cut(s) 385
MnlI CCTC 5 cut(s) 95, 239, 308, 329, 664
MseI TTAA 3 cut(s) 200, 218, 767
MspI CCGG 2 cut(s) 379, 576
NcoI CCATGG 2 cut(s) 285, 727
NdeI CATATG 1 cut(s) 41
NdeII GATC 2 cut(s) 331, 482
NlaIII CATG 4 cut(s) 131, 289, 401, 731
NlaIV GGNNCC 1 cut(s) 165
NmuCI GTSAC 2 cut(s) 388, 688
NspV TTCGAA 1 cut(s) 123
PciSI GCTCTTC 1 cut(s) 574
PkrI GCNGC 1 cut(s) 458
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PsiI TTATAA 3 cut(s) 74, 264, 423
PspN4I GGNNCC 1 cut(s) 165
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
SapI GCTCTTC 1 cut(s) 574
SaqAI TTAA 3 cut(s) 200, 218, 767
SatI GCNGC 1 cut(s) 457
Sau3AI GATC 2 cut(s) 331, 482
ScaI AGTACT 1 cut(s) 471
SchI GAGTC 1 cut(s) 385
SduI GDGCHC 1 cut(s) 166
SfcI CTRYAG 2 cut(s) 91, 651
SfuI TTCGAA 1 cut(s) 123
SsiI CCGC 1 cut(s) 97
SspI AATATT 1 cut(s) 566
SspMI CTAG 3 cut(s) 23, 533, 626
StyI CCWWGG 2 cut(s) 285, 727
TaaI ACNGT 3 cut(s) 259, 608, 802
TaiI ACGT 1 cut(s) 695
TaqI TCGA 4 cut(s) 65, 123, 334, 354
TatI WGTACW 1 cut(s) 469
Tru1I TTAA 3 cut(s) 200, 218, 767
Tru9I TTAA 3 cut(s) 200, 218, 767
TseFI GTSAC 2 cut(s) 388, 688
TseI GCWGC 1 cut(s) 456
Tsp45I GTSAC 2 cut(s) 388, 688
TspDTI ATGAA 4 cut(s) 294, 386, 672, 720
XspI CTAG 3 cut(s) 23, 533, 626
ZrmI AGTACT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.