Rh5DG071200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010401
Physical Location & Seq
Forward (+)
6163336 .. 6168706
5371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG071200.1

Sequence Viewer

Length: 1374 bp
ATGCTGTCAACCACTACTTCTAGAAACATACTTTCTATGACATTTAGACAAGTAGTGTTAGAACTACTCTGGAGCTTTCAACTAGTTGTTTTCAGACCTGGAACTCAAAGGAATATGAAGGATCTTGAGAACCCCAGAGAGGTCCCTGCATGTGTCACCCTTAGTTCATCAGACGAACGGATTTTATCTGTGCTCGCAGAAGTTGTCTGCATCTCTGCTCTTCAAAATACAGAAAAACAATTTCTTGAAGATTACACGGGCAAAACACCGAGTAAATTCCTTCATTGGTTTCGAAAGCCAGGAAGGACTGTATCAAAAGATTCCTCAGTTGTCATATACAAATTATTTGAAGATGAGATAGTTGAAAATGCCAAGAGTCTGCTCGAACATTTTCATTCCACGAAGAACAGTTTTAAGTCTGTAAAAGCAAAATCAAAGTGGTGGACACCATCTGCACTCTCTAAACTGGAAAAGATTGGTGGTCCAGAATTTAGTTCTTGGACTAGTGAGTATGTTCCAGCTTACAAGCTACAAATTGATGCTAATCGGCTTAAGGATGTGAAATTTGAAGGCTGGAGAAAATGTGGAGAAAATAGTTGGGAAGTTCTTTTGACACACTCCCAGATGGTTGGATTGGCTGATATAATAGATATGTACTATGAAGATCTTTACACCCTGCCTGATAAAGAACTATCTTGTGGTGTTATTGCAAGTTCCACCAACTTATCTAATAAAAAGACGGGCTCGTTCTTATTAAAATTGCTATCTGTCTCCCTTGCAAGTGGAATTCTTCTCGTTGCAATCAGTGCTCTTGGTCAATTTGGTTTACCTTATCTACACAAAGGTGGCAAGTACTTGAGAGAACACAGGTCTATTCCATCGTCTGAAGTTGATAGTGCACTAAATCAGTGTTCAGATGCCATGAAGTTGGAAGCATTTTGCGAATCAGTTGTTAAAAAGATAAAAGATTCTCTGGGTTGGCCAGGTGAGATAACGTTGGAAACAAATGTGGGTGCTTGGACTGGCAAAGTACCAGATTATTTGAGGATGGCTGTTGGCGAAGACGTGCCAATCTCTCCCCCTTTAGATAAAATTGATGAAGACTTGAAAGCGTCGGCACAAGATATTGCTAGTTATCAGGTGGTTCTGTCAGCAGATGGAAAGATAGTGGGATTCCAACCTCTAAGCAGGGCTGCTGTTAACCATTGGGCTGCCAATCCCTTGTCGAAGGAGTTATATGGAGGAAGAAAGCTTTCTCCTGGGTTGACGGAACCTGGTCTCAAGGTCCAGTGCCCAGATGAGGTCGCTGTCATAGAGTTGTTGATGTCAGTAAAACCAGATGCATCATTTGCGTTGGCCCGGCCAGTTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

457

Amino Acids

50.72

Weight (kDa)

7.04

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 995
AclWI GGATC 1 cut(s) 129
AcoI YGGCCR 2 cut(s) 980, 1361
AcsI RAATTY 4 cut(s) 275, 488, 563, 786
AcuI CTGAAG 1 cut(s) 906
AfaI GTAC 3 cut(s) 656, 854, 1032
AfiI CCNNNNNNNGG 2 cut(s) 139, 1300
AflII CTTAAG 1 cut(s) 551
AgsI TTSAA 7 cut(s) 80, 224, 248, 350, 365, 569, 1108
AhlI ACTAGT 2 cut(s) 82, 503
AjiI CACGTC 1 cut(s) 1066
AjnI CCWGG 5 cut(s) 97, 298, 982, 1258, 1273
AleI CACNNNNGTG 1 cut(s) 843
AluBI AGCT 4 cut(s) 75, 521, 529, 1252
AluI AGCT 4 cut(s) 75, 521, 529, 1252
Alw21I GWGCWC 3 cut(s) 195, 811, 901
Alw26I GTCTC 2 cut(s) 775, 1283
Alw44I GTGCAC 1 cut(s) 897
AlwI GGATC 1 cut(s) 129
AoxI GGCC 3 cut(s) 980, 1356, 1361
ApaLI GTGCAC 1 cut(s) 897
ApeKI GCWGC 2 cut(s) 1193, 1211
ApoI RAATTY 4 cut(s) 275, 488, 563, 786
Asp700I GAANNNNTTC 2 cut(s) 390, 1252
AspS9I GGNCC 4 cut(s) 142, 482, 1285, 1357
AsuC2I CCSGG 1 cut(s) 1360
AsuHPI GGTGA 2 cut(s) 148, 998
AsuII TTCGAA 1 cut(s) 292
AvaII GGWCC 3 cut(s) 142, 482, 1285
BaeGI GKGCMC 2 cut(s) 901, 1295
BalI TGGCCA 1 cut(s) 982
BanII GRGCYC 1 cut(s) 746
BbsI GAAGAC 2 cut(s) 1068, 1107
Bbv12I GWGCWC 3 cut(s) 195, 811, 901
BbvI GCAGC 2 cut(s) 1180, 1198
BccI CCATC 5 cut(s) 457, 619, 886, 1042, 1151
BciT130I CCWGG 5 cut(s) 99, 300, 984, 1260, 1275
BcnI CCSGG 1 cut(s) 1360
BcoDI GTCTC 2 cut(s) 775, 1283
BcuI ACTAGT 2 cut(s) 82, 503
BfaI CTAG 4 cut(s) 21, 83, 504, 1131
BfrI CTTAAG 1 cut(s) 551
BglII AGATCT 1 cut(s) 664
BisI GCNGC 2 cut(s) 1194, 1212
BlsI GCNGC 2 cut(s) 1195, 1213
BmcAI AGTACT 1 cut(s) 854
Bme1390I CCNGG 6 cut(s) 99, 300, 984, 1260, 1275, 1360
Bme18I GGWCC 3 cut(s) 142, 482, 1285
BmgBI CACGTC 1 cut(s) 1066
BmgT120I GGNCC 4 cut(s) 142, 482, 1285, 1357
BmiI GGNNCC 2 cut(s) 144, 1272
BmrFI CCNGG 6 cut(s) 99, 300, 984, 1260, 1275, 1360
BmsI GCATC 5 cut(s) 219, 529, 907, 1330, 1352
BpiI GAAGAC 2 cut(s) 1068, 1107
BpmI CTGGAG 2 cut(s) 91, 595
Bpu14I TTCGAA 1 cut(s) 292
BpuEI CTTGAG 3 cut(s) 146, 877, 1265
BpuMI CCSGG 1 cut(s) 1360
BsaBI GATNNNNATC 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 1283
BsaJI CCNNGG 1 cut(s) 1259
BsaXI ACNNNNNCTCC 2 cut(s) 568, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 1300
Bse1I ACTGG 4 cut(s) 471, 1027, 1288, 1364
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 5 cut(s) 99, 300, 984, 1260, 1275
BseDI CCNNGG 1 cut(s) 1259
BseGI GGATG 2 cut(s) 562, 1053
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 2 cut(s) 139, 1300
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 4 cut(s) 471, 1027, 1288, 1364
BseSI GKGCMC 2 cut(s) 901, 1295
BseXI GCAGC 2 cut(s) 1180, 1198
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 3 cut(s) 982, 1358, 1363
BsiHKAI GWGCWC 3 cut(s) 195, 811, 901
BsiSI CCGG 1 cut(s) 1360
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 139, 1300
BsmAI GTCTC 2 cut(s) 775, 1283
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 3 cut(s) 982, 1358, 1363
Bso31I GGTCTC 1 cut(s) 1283
Bsp119I TTCGAA 1 cut(s) 292
Bsp1286I GDGCHC 5 cut(s) 195, 746, 811, 901, 1295
Bsp143I GATC 2 cut(s) 121, 664
BspANI GGCC 3 cut(s) 982, 1358, 1363
BspCNI CTCAG 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 144, 1272
BspPI GGATC 1 cut(s) 129
BspQI GCTCTTC 1 cut(s) 225
BspT104I TTCGAA 1 cut(s) 292
BspTI CTTAAG 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 1283
BsrI ACTGG 4 cut(s) 471, 1027, 1288, 1364
BssECI CCNNGG 1 cut(s) 1259
BssMI GATC 2 cut(s) 121, 664
Bst2UI CCWGG 5 cut(s) 99, 300, 984, 1260, 1275
Bst4CI ACNGT 2 cut(s) 310, 410
Bst6I CTCTTC 1 cut(s) 225
BstAFI CTTAAG 1 cut(s) 551
BstAPI GCANNNNNTGC 2 cut(s) 806, 1349
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 3 cut(s) 161, 325, 1184
BstF5I GGATG 2 cut(s) 562, 1053
BstKTI GATC 2 cut(s) 124, 667
BstMAI GTCTC 2 cut(s) 775, 1283
BstMBI GATC 2 cut(s) 121, 664
BstMWI GCNNNNNNNGC 2 cut(s) 806, 1349
BstNI CCWGG 5 cut(s) 99, 300, 984, 1260, 1275
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 6 cut(s) 97, 298, 982, 1258, 1273, 1358
BstSLI GKGCMC 2 cut(s) 901, 1295
BstV1I GCAGC 2 cut(s) 1180, 1198
BstV2I GAAGAC 2 cut(s) 1068, 1107
BstX2I RGATCY 2 cut(s) 121, 664
BstXI CCANNNNNNTGG 2 cut(s) 629, 928
BstYI RGATCY 2 cut(s) 121, 664
BsuRI GGCC 3 cut(s) 982, 1358, 1363
BtrI CACGTC 1 cut(s) 1066
BtsCI GGATG 2 cut(s) 562, 1053
BtsIMutI CAGTG 3 cut(s) 811, 914, 1295
Cac8I GCNNGC 1 cut(s) 195
Cfr13I GGNCC 4 cut(s) 142, 482, 1285, 1357
CseI GACGC 1 cut(s) 1101
CsiI ACCWGGT 1 cut(s) 1273
Csp6I GTAC 3 cut(s) 655, 853, 1031
CviAII CATG 2 cut(s) 150, 922
CviQI GTAC 3 cut(s) 655, 853, 1031
DdeI CTNAG 3 cut(s) 161, 325, 1184
DpnI GATC 2 cut(s) 123, 666
DpnII GATC 2 cut(s) 121, 664
EaeI YGGCCR 2 cut(s) 980, 1361
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 746
Eco31I GGTCTC 1 cut(s) 1283
Eco47I GGWCC 3 cut(s) 142, 482, 1285
Eco57I CTGAAG 1 cut(s) 906
EcoO109I RGGNCCY 1 cut(s) 142
EcoRI GAATTC 1 cut(s) 786
EcoRII CCWGG 5 cut(s) 97, 298, 982, 1258, 1273
EcoT22I ATGCAT 1 cut(s) 1345
EcoT38I GRGCYC 1 cut(s) 746
FaeI CATG 2 cut(s) 153, 925
FaqI GGGAC 1 cut(s) 128
FatI CATG 2 cut(s) 149, 921
Fnu4HI GCNGC 2 cut(s) 1194, 1212
FokI GGATG 2 cut(s) 569, 1060
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 2 cut(s) 1194, 1212
FspBI CTAG 4 cut(s) 21, 83, 504, 1131
GluI GCNGC 2 cut(s) 1194, 1212
GsuI CTGGAG 2 cut(s) 91, 595
HaeIII GGCC 3 cut(s) 982, 1358, 1363
HapII CCGG 1 cut(s) 1360
HgaI GACGC 1 cut(s) 1101
Hin1II CATG 2 cut(s) 153, 925
HincII GTYRAC 3 cut(s) 9, 1201, 1266
HindII GTYRAC 3 cut(s) 9, 1201, 1266
HindIII AAGCTT 1 cut(s) 1250
HinfI GANTC 5 cut(s) 320, 376, 944, 968, 1173
HpaI GTTAAC 1 cut(s) 1201
HpaII CCGG 1 cut(s) 1360
HphI GGTGA 2 cut(s) 148, 998
Hpy166II GTNNAC 6 cut(s) 9, 444, 827, 899, 1201, 1266
Hpy188I TCNGA 4 cut(s) 95, 172, 886, 916
Hpy188III TCNNGA 5 cut(s) 21, 70, 125, 245, 485
Hpy8I GTNNAC 6 cut(s) 9, 444, 827, 899, 1201, 1266
Hpy99I CGWCG 1 cut(s) 1117
HpyAV CCTTC 5 cut(s) 112, 290, 297, 563, 1222
HpyCH4III ACNGT 2 cut(s) 310, 410
HpyCH4IV ACGT 2 cut(s) 995, 1065
HpyCH4V TGCA 8 cut(s) 149, 210, 455, 710, 779, 800, 899, 1343
HpyF10VI GCNNNNNNNGC 2 cut(s) 806, 1349
HpyF3I CTNAG 3 cut(s) 161, 325, 1184
HpySE526I ACGT 2 cut(s) 995, 1065
Hsp92II CATG 2 cut(s) 153, 925
KspAI GTTAAC 1 cut(s) 1201
Kzo9I GATC 2 cut(s) 121, 664
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 2 cut(s) 1180, 1198
LweI GCATC 5 cut(s) 219, 529, 907, 1330, 1352
MabI ACCWGGT 1 cut(s) 1273
MaeI CTAG 4 cut(s) 21, 83, 504, 1131
MaeII ACGT 2 cut(s) 995, 1065
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 123, 666
MboI GATC 2 cut(s) 121, 664
MboII GAAGA 9 cut(s) 212, 260, 362, 415, 674, 782, 1073, 1112, 1257
MflI RGATCY 2 cut(s) 121, 664
MhlI GDGCHC 5 cut(s) 195, 746, 811, 901, 1295
MlsI TGGCCA 1 cut(s) 982
MluNI TGGCCA 1 cut(s) 982
MlyI GAGTC 1 cut(s) 385
MmeI TCCRAC 4 cut(s) 610, 909, 978, 1201
MnlI CCTC 6 cut(s) 133, 334, 1038, 1191, 1235, 1294
Mox20I TGGCCA 1 cut(s) 982
Mph1103I ATGCAT 1 cut(s) 1345
MroXI GAANNNNTTC 2 cut(s) 390, 1252
MscI TGGCCA 1 cut(s) 982
MseI TTAA 5 cut(s) 414, 552, 755, 954, 1200
MslI CAYNNNNRTG 1 cut(s) 843
Msp20I TGGCCA 1 cut(s) 982
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 1 cut(s) 1360
MspR9I CCNGG 6 cut(s) 99, 300, 984, 1260, 1275, 1360
MvaI CCWGG 5 cut(s) 99, 300, 984, 1260, 1275
MwoI GCNNNNNNNGC 2 cut(s) 806, 1349
NciI CCSGG 1 cut(s) 1360
NdeII GATC 2 cut(s) 121, 664
NlaIII CATG 2 cut(s) 153, 925
NlaIV GGNNCC 2 cut(s) 144, 1272
NmuCI GTSAC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 1345
NspI RCATGY 1 cut(s) 153
NspV TTCGAA 1 cut(s) 292
OliI CACNNNNGTG 1 cut(s) 843
PciSI GCTCTTC 1 cut(s) 225
PdmI GAANNNNTTC 2 cut(s) 390, 1252
PfeI GAWTC 4 cut(s) 320, 944, 968, 1173
PkrI GCNGC 2 cut(s) 1195, 1213
PleI GAGTC 1 cut(s) 384
PpsI GAGTC 1 cut(s) 384
PpuMI RGGWCCY 1 cut(s) 142
Psp1406I AACGTT 1 cut(s) 995
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 5 cut(s) 97, 298, 982, 1258, 1273
PspGI CCWGG 5 cut(s) 97, 298, 982, 1258, 1273
PspN4I GGNNCC 2 cut(s) 144, 1272
PspPI GGNCC 4 cut(s) 142, 482, 1285, 1357
PspPPI RGGWCCY 1 cut(s) 142
PsuI RGATCY 2 cut(s) 121, 664
RsaI GTAC 3 cut(s) 656, 854, 1032
RsaNI GTAC 3 cut(s) 655, 853, 1031
RseI CAYNNNNRTG 1 cut(s) 843
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 5 cut(s) 414, 552, 755, 954, 1200
SatI GCNGC 2 cut(s) 1194, 1212
Sau3AI GATC 2 cut(s) 121, 664
Sau96I GGNCC 4 cut(s) 142, 482, 1285, 1357
ScaI AGTACT 1 cut(s) 854
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 6 cut(s) 99, 300, 984, 1260, 1275, 1360
SduI GDGCHC 5 cut(s) 195, 746, 811, 901, 1295
SexAI ACCWGGT 1 cut(s) 1273
SfaNI GCATC 5 cut(s) 219, 529, 907, 1330, 1352
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 3 cut(s) 142, 482, 1285
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 4 cut(s) 125, 551, 856, 1280
SmoI CTYRAG 4 cut(s) 125, 551, 856, 1280
SpeI ACTAGT 2 cut(s) 82, 503
SspMI CTAG 4 cut(s) 21, 83, 504, 1131
StyD4I CCNGG 6 cut(s) 97, 298, 982, 1258, 1273, 1358
TaaI ACNGT 2 cut(s) 310, 410
TaiI ACGT 2 cut(s) 998, 1068
TaqI TCGA 4 cut(s) 292, 384, 1226, 1369
TatI WGTACW 2 cut(s) 654, 852
TfiI GAWTC 4 cut(s) 320, 944, 968, 1173
Tru1I TTAA 5 cut(s) 414, 552, 755, 954, 1200
Tru9I TTAA 5 cut(s) 414, 552, 755, 954, 1200
TscAI CASTG 3 cut(s) 811, 914, 1295
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 2 cut(s) 1193, 1211
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 7 cut(s) 131, 156, 272, 383, 675, 938, 1113
TspGWI ACGGA 2 cut(s) 193, 1283
TspRI CASTG 3 cut(s) 811, 914, 1295
Vha464I CTTAAG 1 cut(s) 551
VneI GTGCAC 1 cut(s) 897
VpaK11BI GGWCC 3 cut(s) 142, 482, 1285
XapI RAATTY 4 cut(s) 275, 488, 563, 786
XbaI TCTAGA 1 cut(s) 20
XceI RCATGY 1 cut(s) 153
XmnI GAANNNNTTC 2 cut(s) 390, 1252
XspI CTAG 4 cut(s) 21, 83, 504, 1131
ZrmI AGTACT 1 cut(s) 854
Zsp2I ATGCAT 1 cut(s) 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.