Rh6AG245000

28 kDa heat- and acid-stable phosphoprotein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018247
Physical Location & Seq
Forward (+)
43352310 .. 43363398
11089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG245000.1

Sequence Viewer

Length: 495 bp
ATGGGAGCTGTTAGATGTGCTTACGAGGAGTGTGATCCTGGGTTCGGAGAGAGGGCGACATGGGGATCTGATGGGGATGAGGCGGGTTGTCCAATTGGACTTCCCGATTATCTTCTAGGTCCGATTGGGTGGTATGATGTGAAGAAGGAGGCGTCTGATGGTTGCTCCGGTGATGGGTTTACGGTTTGGCCATCCGAAAGCTGCTTTTATGGTGCGCAGGCGGAGGTGTGGGCGGCTGGGGCTGGAAGGAATTCCAGCTCCGAACTGGATCGGGTCTGCCGTCTGGGAGGCTTACTTGTGTACGCTGGGGTGACTGATCTGTGGTGGCACCAAGGAGCGACGGCGACAGCGGTGTTGCTGCGGGGTTGGTGGAAGGTTGTTAATGCAGAAAGGGAGGAGATTGAAAAACAAAAGGCTCATGAGCGATACATGAGGTTGCAGGAGCAGGGAAAAACTGAACAAGCAAAGAAAGATTTAGGTAATATGCAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

164

Amino Acids

18.03

Weight (kDa)

4.81

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP28 PF10252 130 - 160 1e-11 Casein kinase substrate phosphoprotein PP28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018247)

Species Orthologous Gene IDs
rosa_laevigata RLG00000035741
rosa_roxburghii Rroxscaffold_7G00199610
rosa_samantha Rh1AG094200 Rh1CG397700 Rh2BG309400 Rh4BG396000 Rh4CG411100 Rh6AG245000 Rh7AG015700 Rh7BG228400
rosa_wichuraiana Rw1G007350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 327
AciI CCGC 5 cut(s) 83, 221, 233, 350, 361
AclWI GGATC 3 cut(s) 29, 73, 276
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 2 cut(s) 44, 174
AgsI TTSAA 1 cut(s) 404
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 3 cut(s) 8, 201, 258
AluI AGCT 3 cut(s) 8, 201, 258
AlwI GGATC 3 cut(s) 29, 73, 276
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 201, 358
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 1 cut(s) 250
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 182, 322
AvaII GGWCC 1 cut(s) 119
BalI TGGCCA 1 cut(s) 190
BanI GGYRCC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 188, 345
BccI CCATC 4 cut(s) 65, 152, 167, 199
BceAI ACGGC 2 cut(s) 264, 357
BciT130I CCWGG 1 cut(s) 39
BfaI CTAG 1 cut(s) 116
BisI GCNGC 3 cut(s) 202, 234, 359
BlsI GCNGC 3 cut(s) 203, 235, 360
Bme1390I CCNGG 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 329
BmrFI CCNGG 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 38, 331
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 174
Bse1I ACTGG 1 cut(s) 270
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 2 cut(s) 38, 331
BseGI GGATG 2 cut(s) 82, 191
BseLI CCNNNNNNNGG 2 cut(s) 44, 174
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 2 cut(s) 41, 410
BseXI GCAGC 2 cut(s) 188, 345
BseYI CCCAGC 2 cut(s) 236, 305
BshFI GGCC 1 cut(s) 190
BshNI GGYRCC 1 cut(s) 327
BsiSI CCGG 1 cut(s) 168
BslI CCNNNNNNNGG 2 cut(s) 44, 174
BsnI GGCC 1 cut(s) 190
Bsp143I GATC 4 cut(s) 34, 65, 268, 316
BspACI CCGC 5 cut(s) 83, 221, 233, 350, 361
BspANI GGCC 1 cut(s) 190
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 1 cut(s) 329
BspPI GGATC 3 cut(s) 29, 73, 276
BspT107I GGYRCC 1 cut(s) 327
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 2 cut(s) 38, 331
BssMI GATC 4 cut(s) 34, 65, 268, 316
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 219
BstF5I GGATG 2 cut(s) 82, 191
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 4 cut(s) 37, 68, 271, 319
BstMBI GATC 4 cut(s) 34, 65, 268, 316
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 188, 345
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 1 cut(s) 190
BtsCI GGATG 2 cut(s) 82, 191
Cac8I GCNNGC 1 cut(s) 219
CciI TCATGA 1 cut(s) 418
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 1 cut(s) 141
Csp6I GTAC 1 cut(s) 301
CviAII CATG 3 cut(s) 60, 419, 430
CviJI RGCY 8 cut(s) 8, 190, 201, 236, 242, 258, 291, 416
CviKI_1 RGCY 8 cut(s) 8, 190, 201, 236, 242, 258, 291, 416
CviQI GTAC 1 cut(s) 301
DpnI GATC 4 cut(s) 36, 67, 270, 318
DpnII GATC 4 cut(s) 34, 65, 268, 316
EaeI YGGCCR 1 cut(s) 188
EciI GGCGGA 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 3 cut(s) 63, 422, 433
FaiI YATR 6 cut(s) 61, 135, 210, 420, 431, 485
FatI CATG 3 cut(s) 59, 418, 429
FauI CCCGC 2 cut(s) 76, 354
Fnu4HI GCNGC 3 cut(s) 202, 234, 359
FokI GGATG 2 cut(s) 89, 178
Fsp4HI GCNGC 3 cut(s) 202, 234, 359
FspBI CTAG 1 cut(s) 116
FspI TGCGCA 1 cut(s) 216
GlaI GCGC 1 cut(s) 216
GluI GCNGC 3 cut(s) 202, 234, 359
GsaI CCCAGC 2 cut(s) 240, 309
HaeIII GGCC 1 cut(s) 190
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 141
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 3 cut(s) 63, 422, 433
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 182, 322
Hpy166II GTNNAC 2 cut(s) 180, 301
Hpy188I TCNGA 6 cut(s) 47, 70, 123, 157, 196, 262
Hpy188III TCNNGA 2 cut(s) 104, 419
Hpy8I GTNNAC 2 cut(s) 180, 301
Hpy99I CGWCG 1 cut(s) 343
HpyAV CCTTC 3 cut(s) 139, 240, 367
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 386, 439, 487
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 3 cut(s) 63, 422, 433
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 4 cut(s) 34, 65, 268, 316
LmnI GCTCC 5 cut(s) 5, 170, 263, 335, 442
Lsp1109I GCAGC 2 cut(s) 188, 345
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 1 cut(s) 310
MalI GATC 4 cut(s) 36, 67, 270, 318
MboI GATC 4 cut(s) 34, 65, 268, 316
MboII GAAGA 2 cut(s) 104, 154
MfeI CAATTG 1 cut(s) 93
MflI RGATCY 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 190
MluCI AATT 3 cut(s) 93, 250, 490
MluNI TGGCCA 1 cut(s) 190
MnlI CCTC 8 cut(s) 19, 45, 73, 142, 217, 281, 388, 426
Mox20I TGGCCA 1 cut(s) 190
MroXI GAANNNNTTC 1 cut(s) 250
MscI TGGCCA 1 cut(s) 190
MseI TTAA 1 cut(s) 381
Msp20I TGGCCA 1 cut(s) 190
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 39
MunI CAATTG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 39
MwoI GCNNNNNNNGC 1 cut(s) 239
NdeII GATC 4 cut(s) 34, 65, 268, 316
NlaIII CATG 3 cut(s) 63, 422, 433
NlaIV GGNNCC 1 cut(s) 329
NmuCI GTSAC 1 cut(s) 310
NsbI TGCGCA 1 cut(s) 216
PagI TCATGA 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 250
PkrI GCNGC 3 cut(s) 203, 235, 360
Psp6I CCWGG 1 cut(s) 37
PspFI CCCAGC 2 cut(s) 236, 305
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 329
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 65
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 3 cut(s) 202, 234, 359
Sau3AI GATC 4 cut(s) 34, 65, 268, 316
Sau96I GGNCC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 39
SetI ASST 8 cut(s) 10, 121, 203, 228, 260, 378, 437, 481
SinI GGWCC 1 cut(s) 119
Sse9I AATT 3 cut(s) 93, 250, 490
SsiI CCGC 5 cut(s) 83, 221, 233, 350, 361
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 184
TasI AATT 3 cut(s) 93, 250, 490
TauI GCSGC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 201, 358
Tsp45I GTSAC 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 250
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.