Rw1G007350

Nucleotide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018247
Physical Location & Seq
Forward (+)
15503108 .. 15506798
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G007350.1

Sequence Viewer

Length: 681 bp
ATGGACGGCCACACCCTTGGAAGACATTTTCAAGTGACTAGTCTGGTTAGGAAGGTTTCCCCCAGTTGTGAAGACAGCAGTGCCCGTAGATGGGAGGAACAGGAACATAATCATCCCGACATATTGCTTCTGCAGATTGGAAACACATTCTGCAAACTTCCTGGTGGACGACTGAAGACTGGGGACAATGGCTTGGACGGAGACGTGGTTGAAACGGTATGTGGCTGGCATGTTGTAACCCAGATCCAAATTGCTCTGGTTACTAGGAGGTTCCGGTGGTTGGCTGGGTTGCCTGGCGGTGCAAGTGCTTACGAGGAGTGTGATCCTGGGTTCGGAGAAAGGGCGGCATGGGGATCTGATGGGGATGAGGCGGGTTGTCCAATTGGACTTCCCGATTATCTTCTGGGTCCGATTGGGTGGTCGGATGTGAAGAAGGAGGCGTCTGATGGTTGCTCCGGTGATGGGTTTACGGTTTGGCCATCCGAAAGCTGCTTTCATGGTGCGCAAGCGGAGGTGTGGGTGGATGGGGCTGGAAGGAATTCCAGATCCGAACTGGATCGGGTCTGCCTTCTGGGAGGCTTACCTGTGTACGATGGGGTGACTGATCTGTGGTGGCACCAAGGGGCGACGGCGACGGCGGTGCTGCTGCGGGGTTGGTGGTGCGTGTCTGCTACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005849 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010608 GO:0010628 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0017091 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0031323 GO:0031325 GO:0031437 GO:0031439 GO:0031440 GO:0031442 GO:0031974 GO:0031981 GO:0032991 GO:0034622 GO:0034641 GO:0042382 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050684 GO:0050685 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051259 GO:0051262 GO:0051290 GO:0051291 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0110104 GO:1900363 GO:1900365 GO:1901360 GO:1901363 GO:1901532 GO:1901534 GO:1903311 GO:1903313 GO:1903706 GO:1903708 GO:1905456 GO:1905458 GO:1990120 GO:2000026 GO:2000736 GO:2000738 GO:2000973 GO:2000975
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
NUDIX_2

Protein Analysis

226

Amino Acids

24.45

Weight (kDa)

4.9

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX_2 PF13869 34 - 66 5.3e-06 Nucleotide hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018247)

Species Orthologous Gene IDs
rosa_laevigata RLG00000035741
rosa_roxburghii Rroxscaffold_7G00199610
rosa_samantha Rh1AG094200 Rh1CG397700 Rh2BG309400 Rh4BG396000 Rh4CG411100 Rh6AG245000 Rh7AG015700 Rh7BG228400
rosa_wichuraiana Rw1G007350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 615
AciI CCGC 6 cut(s) 297, 344, 371, 509, 638, 649
AclWI GGATC 5 cut(s) 238, 317, 361, 540, 564
AcoI YGGCCR 2 cut(s) 7, 476
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 1 cut(s) 194
AcyI GRCGYC 1 cut(s) 440
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 5 cut(s) 90, 91, 280, 332, 462
AgsI TTSAA 2 cut(s) 32, 212
AhlI ACTAGT 1 cut(s) 38
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 3 cut(s) 160, 292, 325
AluBI AGCT 1 cut(s) 489
AluI AGCT 1 cut(s) 489
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 5 cut(s) 238, 317, 361, 540, 564
AoxI GGCC 2 cut(s) 7, 476
ApeKI GCWGC 3 cut(s) 489, 643, 646
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 538
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 407
AsuHPI GGTGA 2 cut(s) 470, 610
AvaII GGWCC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 85
BalI TGGCCA 1 cut(s) 478
BanI GGYRCC 1 cut(s) 615
BbsI GAAGAC 3 cut(s) 28, 78, 182
BbvI GCAGC 3 cut(s) 476, 630, 633
BccI CCATC 7 cut(s) 84, 353, 440, 455, 487, 518, 587
BceAI ACGGC 3 cut(s) 22, 645, 651
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BciT130I CCWGG 3 cut(s) 162, 294, 327
BcoDI GTCTC 1 cut(s) 195
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 3 cut(s) 39, 264, 679
BfmI CTRYAG 1 cut(s) 131
BisI GCNGC 4 cut(s) 345, 490, 644, 647
BlsI GCNGC 4 cut(s) 346, 491, 645, 648
Bme1390I CCNGG 3 cut(s) 162, 294, 327
Bme18I GGWCC 1 cut(s) 407
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 3 cut(s) 272, 408, 617
BmrFI CCNGG 3 cut(s) 162, 294, 327
BmrI ACTGGG 2 cut(s) 57, 189
BmuI ACTGGG 2 cut(s) 57, 189
BpiI GAAGAC 3 cut(s) 28, 78, 182
BsaHI GRCGYC 1 cut(s) 440
BsaJI CCNNGG 3 cut(s) 16, 326, 619
BsaWI WCCGGW 2 cut(s) 273, 455
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 91, 280, 332, 462
Bse1I ACTGG 3 cut(s) 63, 184, 558
BseBI CCWGG 3 cut(s) 162, 294, 327
BseDI CCNNGG 3 cut(s) 16, 326, 619
BseGI GGATG 5 cut(s) 112, 370, 430, 479, 529
BseLI CCNNNNNNNGG 5 cut(s) 90, 91, 280, 332, 462
BseNI ACTGG 3 cut(s) 63, 184, 558
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 85
BseXI GCAGC 3 cut(s) 476, 630, 633
BseYI CCCAGC 1 cut(s) 284
BshFI GGCC 2 cut(s) 9, 478
BshNI GGYRCC 1 cut(s) 615
BsiSI CCGG 2 cut(s) 274, 456
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 5 cut(s) 90, 91, 280, 332, 462
BsmAI GTCTC 1 cut(s) 195
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 2 cut(s) 9, 478
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 6 cut(s) 243, 322, 353, 545, 556, 604
BspACI CCGC 6 cut(s) 297, 344, 371, 509, 638, 649
BspANI GGCC 2 cut(s) 9, 478
BspLI GGNNCC 3 cut(s) 272, 408, 617
BspMAI CTGCAG 1 cut(s) 135
BspPI GGATC 5 cut(s) 238, 317, 361, 540, 564
BspT107I GGYRCC 1 cut(s) 615
BsrI ACTGG 3 cut(s) 63, 184, 558
BssECI CCNNGG 3 cut(s) 16, 326, 619
BssMI GATC 6 cut(s) 243, 322, 353, 545, 556, 604
BssNI GRCGYC 1 cut(s) 440
BssT1I CCWWGG 2 cut(s) 16, 619
Bst2UI CCWGG 3 cut(s) 162, 294, 327
Bst4CI ACNGT 3 cut(s) 217, 472, 676
BstACI GRCGYC 1 cut(s) 440
BstC8I GCNNGC 2 cut(s) 227, 507
BstF5I GGATG 5 cut(s) 112, 370, 430, 479, 529
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 6 cut(s) 246, 325, 356, 548, 559, 607
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 6 cut(s) 243, 322, 353, 545, 556, 604
BstNI CCWGG 3 cut(s) 162, 294, 327
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 160, 292, 325
BstSFI CTRYAG 1 cut(s) 131
BstSLI GKGCMC 1 cut(s) 85
BstV1I GCAGC 3 cut(s) 476, 630, 633
BstV2I GAAGAC 3 cut(s) 28, 78, 182
BstX2I RGATCY 3 cut(s) 243, 353, 545
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 3 cut(s) 243, 353, 545
BsuRI GGCC 2 cut(s) 9, 478
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 5 cut(s) 112, 370, 430, 479, 529
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 2 cut(s) 227, 507
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 1 cut(s) 429
Csp6I GTAC 1 cut(s) 589
CviAII CATG 3 cut(s) 230, 348, 497
CviJI RGCY 8 cut(s) 9, 192, 225, 284, 478, 489, 530, 579
CviKI_1 RGCY 8 cut(s) 9, 192, 225, 284, 478, 489, 530, 579
CviQI GTAC 1 cut(s) 589
DpnI GATC 6 cut(s) 245, 324, 355, 547, 558, 606
DpnII GATC 6 cut(s) 243, 322, 353, 545, 556, 604
EaeI YGGCCR 2 cut(s) 7, 476
Eco130I CCWWGG 2 cut(s) 16, 619
Eco47I GGWCC 1 cut(s) 407
Eco57I CTGAAG 1 cut(s) 194
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 3 cut(s) 160, 292, 325
EcoT14I CCWWGG 2 cut(s) 16, 619
ErhI CCWWGG 2 cut(s) 16, 619
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 3 cut(s) 233, 351, 500
FaiI YATR 6 cut(s) 108, 122, 220, 231, 349, 498
FaqI GGGAC 1 cut(s) 197
FatI CATG 3 cut(s) 229, 347, 496
FauI CCCGC 2 cut(s) 364, 642
Fnu4HI GCNGC 4 cut(s) 345, 490, 644, 647
FokI GGATG 5 cut(s) 99, 377, 437, 466, 536
Fsp4HI GCNGC 4 cut(s) 345, 490, 644, 647
FspBI CTAG 3 cut(s) 39, 264, 679
FspI TGCGCA 1 cut(s) 504
GlaI GCGC 1 cut(s) 504
GluI GCNGC 4 cut(s) 345, 490, 644, 647
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 2 cut(s) 9, 478
HapII CCGG 2 cut(s) 274, 456
HgaI GACGC 1 cut(s) 429
HhaI GCGC 1 cut(s) 505
Hin1I GRCGYC 1 cut(s) 440
Hin1II CATG 3 cut(s) 233, 351, 500
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HpaII CCGG 2 cut(s) 274, 456
HphI GGTGA 2 cut(s) 470, 610
Hpy166II GTNNAC 3 cut(s) 167, 468, 589
Hpy188I TCNGA 7 cut(s) 335, 358, 411, 424, 445, 484, 550
Hpy188III TCNNGA 3 cut(s) 116, 392, 543
Hpy8I GTNNAC 3 cut(s) 167, 468, 589
Hpy99I CGWCG 2 cut(s) 631, 637
HpyAV CCTTC 4 cut(s) 46, 427, 528, 578
HpyCH4III ACNGT 3 cut(s) 217, 472, 676
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 3 cut(s) 133, 153, 302
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 440
Hsp92II CATG 3 cut(s) 233, 351, 500
HspAI GCGC 1 cut(s) 503
Kzo9I GATC 6 cut(s) 243, 322, 353, 545, 556, 604
LmnI GCTCC 1 cut(s) 458
Lsp1109I GCAGC 3 cut(s) 476, 630, 633
MaeI CTAG 3 cut(s) 39, 264, 679
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 4 cut(s) 34, 235, 259, 598
MalI GATC 6 cut(s) 245, 324, 355, 547, 558, 606
MboI GATC 6 cut(s) 243, 322, 353, 545, 556, 604
MboII GAAGA 5 cut(s) 33, 83, 187, 392, 442
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 3 cut(s) 243, 353, 545
MhlI GDGCHC 1 cut(s) 85
MlsI TGGCCA 1 cut(s) 478
MluCI AATT 3 cut(s) 249, 381, 538
MluNI TGGCCA 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 7 cut(s) 88, 261, 307, 361, 430, 505, 569
Mox20I TGGCCA 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 478
Msp20I TGGCCA 1 cut(s) 478
MspI CCGG 2 cut(s) 274, 456
MspR9I CCNGG 3 cut(s) 162, 294, 327
MunI CAATTG 1 cut(s) 381
MvaI CCWGG 3 cut(s) 162, 294, 327
NdeII GATC 6 cut(s) 243, 322, 353, 545, 556, 604
NlaIII CATG 3 cut(s) 233, 351, 500
NlaIV GGNNCC 3 cut(s) 272, 408, 617
NmuCI GTSAC 2 cut(s) 34, 598
NsbI TGCGCA 1 cut(s) 504
NspI RCATGY 1 cut(s) 233
PdmI GAANNNNTTC 1 cut(s) 538
PkrI GCNGC 4 cut(s) 346, 491, 645, 648
Psp6I CCWGG 3 cut(s) 160, 292, 325
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 3 cut(s) 160, 292, 325
PspN4I GGNNCC 3 cut(s) 272, 408, 617
PspPI GGNCC 1 cut(s) 407
PstI CTGCAG 1 cut(s) 135
PsuI RGATCY 3 cut(s) 243, 353, 545
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
SatI GCNGC 4 cut(s) 345, 490, 644, 647
Sau3AI GATC 6 cut(s) 243, 322, 353, 545, 556, 604
Sau96I GGNCC 1 cut(s) 407
ScrFI CCNGG 3 cut(s) 162, 294, 327
SduI GDGCHC 1 cut(s) 85
SetI ASST 6 cut(s) 57, 207, 272, 491, 516, 586
SfcI CTRYAG 1 cut(s) 131
SinI GGWCC 1 cut(s) 407
SpeI ACTAGT 1 cut(s) 38
Sse9I AATT 3 cut(s) 249, 381, 538
SsiI CCGC 6 cut(s) 297, 344, 371, 509, 638, 649
SspMI CTAG 3 cut(s) 39, 264, 679
StyD4I CCNGG 3 cut(s) 160, 292, 325
StyI CCWWGG 2 cut(s) 16, 619
TaaI ACNGT 3 cut(s) 217, 472, 676
TaiI ACGT 1 cut(s) 207
TasI AATT 3 cut(s) 249, 381, 538
TauI GCSGC 1 cut(s) 347
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 2 cut(s) 34, 598
TseI GCWGC 3 cut(s) 489, 643, 646
Tsp45I GTSAC 2 cut(s) 34, 598
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 1 cut(s) 538
XceI RCATGY 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 538
XspI CTAG 3 cut(s) 39, 264, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.