Rh6AG475700

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009924
Physical Location & Seq
Reverse (-)
65760856 .. 65762509
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG475700.1

Sequence Viewer

Length: 471 bp
ATGGAAGGAGAGTACAGTTGTTCGTCATCGTCTTCTTCCTCGACCACGAGCTTAGAGAAGCGCAAGAAGAGACAACATGAGCACGATGATAACCAGGAAAAGCCCTACAGAGGAATAAGGATGAGAAAGTGGGGCAAGTGGGTGGCCGAGATTAGAGAACCCAACAAGCGGTCCAGGATTTGGCTTGGTTCGTACACAACTCCTGTCGCCGCCGCCAGAGCCTACGACACCGCCGTTTTCTACCTCCGAGGCCCTTCTGCGAGACTCAACTTCCCGGAGTTAGTTTTCCAAGAAGGCCAGCTGCACGACATGTCGTCCGCTTCCATACGCAAGAAGGCCACCGAGGTTGGGGCCAAAGTCGACGCTCTCCAAACTGCTCTCCGGTCGCCGTCGTCGCAGTCGAAAACTGGCTCCCAGATTAAGCCTGATTTGAACGAATACCCGGACCCGGAAAACTCCGATGACAACTGA

Protein Analysis

156

Amino Acids

17.64

Weight (kDa)

9.18

Isoelectric Point (pI)

57.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 36 - 84 7.8e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009924)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G46768 AT1G46768 AT1G46768 AT4G06746
fragaria_vesca FvH4_2g34020
malus_domestica MD08G1096000.v1.1 MD15G1078900.v1.1
prunus_persica Prupe.1G432000_v2.0.a1
pyrus_communis pycom08g07820 pycom15g07350
rosa_chinensis RchiOBHm_Chr6g0308371
rosa_laevigata RLG00000010647
rosa_multiflora Rmu_sc0005523.1_g000015
rosa_roxburghii Rroxscaffold_7G00160190
rosa_rugosa Rorug06G0366300 Rorug06G0366400
rosa_samantha Rh6AG475700 Rh6BG486000 Rh6CG491200 Rh6DG477500
rosa_wichuraiana Rw6G041460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AccI GTMKAC 1 cut(s) 360
AciI CCGC 5 cut(s) 169, 210, 213, 231, 318
AcoI YGGCCR 1 cut(s) 144
AfaI GTAC 2 cut(s) 14, 194
AfiI CCNNNNNNNGG 5 cut(s) 110, 168, 180, 348, 448
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 1 cut(s) 433
AhdI GACNNNNNGTC 1 cut(s) 313
AjnI CCWGG 2 cut(s) 93, 173
AluBI AGCT 2 cut(s) 51, 301
AluI AGCT 2 cut(s) 51, 301
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 64, 256
AoxI GGCC 5 cut(s) 144, 250, 295, 336, 351
ApeKI GCWGC 1 cut(s) 301
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 4 cut(s) 171, 251, 351, 445
AsuC2I CCSGG 3 cut(s) 275, 443, 449
AvaII GGWCC 2 cut(s) 171, 445
BauI CACGAG 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 288
BceAI ACGGC 2 cut(s) 218, 373
BciT130I CCWGG 2 cut(s) 95, 175
BcnI CCSGG 3 cut(s) 275, 443, 449
BcoDI GTCTC 2 cut(s) 64, 256
BfmI CTRYAG 1 cut(s) 106
BisI GCNGC 3 cut(s) 210, 213, 302
BlsI GCNGC 3 cut(s) 211, 214, 303
Bme1390I CCNGG 5 cut(s) 95, 175, 275, 443, 449
Bme18I GGWCC 2 cut(s) 171, 445
BmeRI GACNNNNNGTC 1 cut(s) 313
BmgT120I GGNCC 4 cut(s) 171, 251, 351, 445
BmiI GGNNCC 3 cut(s) 352, 412, 447
BmrFI CCNGG 5 cut(s) 95, 175, 275, 443, 449
BpiI GAAGAC 1 cut(s) 24
BpuMI CCSGG 3 cut(s) 275, 443, 449
BsaJI CCNNGG 2 cut(s) 247, 342
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 5 cut(s) 110, 168, 180, 348, 448
Bse1I ACTGG 1 cut(s) 412
BseBI CCWGG 2 cut(s) 95, 175
BseDI CCNNGG 2 cut(s) 247, 342
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 5 cut(s) 110, 168, 180, 348, 448
BseNI ACTGG 1 cut(s) 412
BseXI GCAGC 1 cut(s) 288
BsgI GTGCAG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 386
BshFI GGCC 5 cut(s) 146, 252, 297, 338, 353
BsiEI CGRYCG 1 cut(s) 386
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 4 cut(s) 275, 382, 443, 449
BslI CCNNNNNNNGG 5 cut(s) 110, 168, 180, 348, 448
BsmAI GTCTC 2 cut(s) 64, 256
BsnI GGCC 5 cut(s) 146, 252, 297, 338, 353
Bsp1286I GDGCHC 1 cut(s) 84
BspACI CCGC 5 cut(s) 169, 210, 213, 231, 318
BspANI GGCC 5 cut(s) 146, 252, 297, 338, 353
BspLI GGNNCC 3 cut(s) 352, 412, 447
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 2 cut(s) 247, 342
BssSI CACGAG 1 cut(s) 46
Bst2BI CACGAG 1 cut(s) 46
Bst2UI CCWGG 2 cut(s) 95, 175
Bst4CI ACNGT 1 cut(s) 17
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 52
BstF5I GGATG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 63
BstMAI GTCTC 2 cut(s) 64, 256
BstMCI CGRYCG 1 cut(s) 386
BstMWI GCNNNNNNNGC 2 cut(s) 218, 394
BstNI CCWGG 2 cut(s) 95, 175
BstNSI RCATGY 1 cut(s) 313
BstSCI CCNGG 5 cut(s) 93, 173, 273, 441, 447
BstSFI CTRYAG 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 24
BsuRI GGCC 5 cut(s) 146, 252, 297, 338, 353
BtsCI GGATG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 299
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 4 cut(s) 171, 251, 351, 445
CseI GACGC 1 cut(s) 371
Csp6I GTAC 2 cut(s) 13, 193
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 2 cut(s) 77, 310
CviQI GTAC 2 cut(s) 13, 193
DdeI CTNAG 1 cut(s) 52
DriI GACNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 62
Eco47I GGWCC 2 cut(s) 171, 445
EcoO109I RGGNCCY 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 93, 173
FaeI CATG 2 cut(s) 80, 313
FaiI YATR 3 cut(s) 78, 311, 326
FatI CATG 2 cut(s) 76, 309
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 210, 213, 302
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 3 cut(s) 210, 213, 302
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 210, 213, 302
HaeIII GGCC 5 cut(s) 146, 252, 297, 338, 353
HapII CCGG 4 cut(s) 275, 382, 443, 449
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 63
Hin1II CATG 2 cut(s) 80, 313
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 264
HpaII CCGG 4 cut(s) 275, 382, 443, 449
Hpy166II GTNNAC 2 cut(s) 195, 361
Hpy188I TCNGA 2 cut(s) 248, 460
Hpy8I GTNNAC 2 cut(s) 195, 361
Hpy99I CGWCG 3 cut(s) 365, 394, 397
HpyAV CCTTC 3 cut(s) 264, 287, 328
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 394
HpyF3I CTNAG 1 cut(s) 52
Hsp92II CATG 2 cut(s) 80, 313
HspAI GCGC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 1 cut(s) 288
MboII GAAGA 3 cut(s) 24, 27, 79
MhlI GDGCHC 1 cut(s) 84
MlyI GAGTC 1 cut(s) 258
MnlI CCTC 5 cut(s) 49, 104, 242, 254, 337
MseI TTAA 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 4 cut(s) 275, 382, 443, 449
MspR9I CCNGG 5 cut(s) 95, 175, 275, 443, 449
MvaI CCWGG 2 cut(s) 95, 175
MwoI GCNNNNNNNGC 2 cut(s) 218, 394
NciI CCSGG 3 cut(s) 275, 443, 449
NlaIII CATG 2 cut(s) 80, 313
NlaIV GGNNCC 3 cut(s) 352, 412, 447
NmeAIII GCCGAG 1 cut(s) 172
NspI RCATGY 1 cut(s) 313
PciI ACATGT 1 cut(s) 309
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 398
PflMI CCANNNNNTGG 1 cut(s) 180
PfoI TCCNGGA 2 cut(s) 173, 273
PkrI GCNGC 3 cut(s) 211, 214, 303
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
PscI ACATGT 1 cut(s) 309
Psp6I CCWGG 2 cut(s) 93, 173
PspGI CCWGG 2 cut(s) 93, 173
PspN4I GGNNCC 3 cut(s) 352, 412, 447
PspPI GGNCC 4 cut(s) 171, 251, 351, 445
PvuII CAGCTG 1 cut(s) 301
RsaI GTAC 2 cut(s) 14, 194
RsaNI GTAC 2 cut(s) 13, 193
SalI GTCGAC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 3 cut(s) 210, 213, 302
Sau96I GGNCC 4 cut(s) 171, 251, 351, 445
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 5 cut(s) 95, 175, 275, 443, 449
SduI GDGCHC 1 cut(s) 84
SetI ASST 4 cut(s) 53, 246, 303, 348
SfcI CTRYAG 1 cut(s) 106
SinI GGWCC 2 cut(s) 171, 445
SsiI CCGC 5 cut(s) 169, 210, 213, 231, 318
StyD4I CCNGG 5 cut(s) 93, 173, 273, 441, 447
TaaI ACNGT 1 cut(s) 17
TaqI TCGA 3 cut(s) 41, 360, 401
TatI WGTACW 1 cut(s) 12
TauI GCSGC 2 cut(s) 212, 215
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TseI GCWGC 1 cut(s) 301
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 171, 445
XceI RCATGY 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.