Rh6BG092200

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017765
Physical Location & Seq
Forward (+)
14560134 .. 14560890
757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG092200.1

Sequence Viewer

Length: 273 bp
ATGGACCGGCACAGCTCCTCATCTGTGGATTCGTTGGCGAAGATTGGGCGGTTCGCCAAGGAGTCGGAAGAGAACATGAAGTTTCTCGCCAAGTGCGATGGGTTTGTGGCGCTGCTCGTCGAGGCTTTTGGCGGCGGCAAGGCCAGTGACGGAGGTCACGGGAGCAACAACATTCGCATTCTCAAGCAAATCATCAGGATCATTCATTTTCTGATTGGAGACATCCAAGAAAATGATGAATTGATGAGATTGATGTTGAAGAAGAGCGGCTAG

Protein Analysis

90

Amino Acids

9.89

Weight (kDa)

7.92

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 7 - 79 6.9e-06 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 267
AciI CCGC 4 cut(s) 49, 132, 135, 267
AclWI GGATC 1 cut(s) 206
AgsI TTSAA 1 cut(s) 259
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 213
AlwI GGATC 1 cut(s) 206
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 4
BbvI GCAGC 1 cut(s) 99
BccI CCATC 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 1 cut(s) 271
BfoI RGCGCY 1 cut(s) 113
BisI GCNGC 4 cut(s) 113, 133, 136, 268
BlsI GCNGC 4 cut(s) 114, 134, 137, 269
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BoxI GACNNNNGTC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 6
Bse1I ACTGG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 7
BseXI GCAGC 1 cut(s) 99
BshFI GGCC 1 cut(s) 143
BsiSI CCGG 1 cut(s) 7
BsmAI GTCTC 1 cut(s) 213
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 1 cut(s) 143
Bsp143I GATC 1 cut(s) 198
BspACI CCGC 4 cut(s) 49, 132, 135, 267
BspANI GGCC 1 cut(s) 143
BspPI GGATC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 267
BsrFI RCCGGY 1 cut(s) 6
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 6
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 57
Bst6I CTCTTC 2 cut(s) 63, 257
BstF5I GGATG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 1 cut(s) 198
BstPAI GACNNNNGTC 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 99
BsuRI GGCC 1 cut(s) 143
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 1 cut(s) 76
CviJI RGCY 4 cut(s) 15, 125, 143, 270
CviKI_1 RGCY 4 cut(s) 15, 125, 143, 270
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
Eam1104I CTCTTC 2 cut(s) 63, 257
EarI CTCTTC 2 cut(s) 63, 257
Eco130I CCWWGG 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 1 cut(s) 79
FaiI YATR 1 cut(s) 77
FatI CATG 1 cut(s) 75
Fnu4HI GCNGC 4 cut(s) 113, 133, 136, 268
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 4 cut(s) 113, 133, 136, 268
FspBI CTAG 1 cut(s) 271
GlaI GCGC 1 cut(s) 111
GluI GCNGC 4 cut(s) 113, 133, 136, 268
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 1 cut(s) 143
HapII CCGG 1 cut(s) 7
HhaI GCGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HinfI GANTC 2 cut(s) 29, 62
HpaII CCGG 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 67, 213
Hpy188III TCNNGA 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 122
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 1 cut(s) 198
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 2 cut(s) 20, 162
LpnPI CCDG 3 cut(s) 20, 157, 181
Lsp1109I GCAGC 1 cut(s) 99
MaeI CTAG 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 146, 155
MalI GATC 1 cut(s) 200
MbiI CCGCTC 1 cut(s) 267
MboI GATC 1 cut(s) 198
MboII GAAGA 3 cut(s) 52, 80, 271
MluCI AATT 1 cut(s) 239
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 3 cut(s) 28, 115, 146
MspI CCGG 1 cut(s) 7
Mva1269I GAATGC 1 cut(s) 177
NdeII GATC 1 cut(s) 198
NlaIII CATG 1 cut(s) 79
NmuCI GTSAC 2 cut(s) 146, 155
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 1 cut(s) 177
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 4 cut(s) 114, 134, 137, 269
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshAI GACNNNNGTC 1 cut(s) 153
PspPI GGNCC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 257
SatI GCNGC 4 cut(s) 113, 133, 136, 268
Sau3AI GATC 1 cut(s) 198
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 71
SetI ASST 2 cut(s) 17, 157
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 1 cut(s) 239
SsiI CCGC 4 cut(s) 49, 132, 135, 267
SspMI CTAG 1 cut(s) 271
StyI CCWWGG 1 cut(s) 57
TaqI TCGA 1 cut(s) 120
TasI AATT 1 cut(s) 239
TauI GCSGC 3 cut(s) 135, 138, 270
TfiI GAWTC 1 cut(s) 29
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 2 cut(s) 146, 155
TseI GCWGC 1 cut(s) 112
Tsp45I GTSAC 2 cut(s) 146, 155
TspDTI ATGAA 3 cut(s) 92, 194, 252
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 4
XspI CTAG 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.