Rh6BG299800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015632
Physical Location & Seq
Reverse (-)
52889422 .. 52890122
701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG299800.1

Sequence Viewer

Length: 645 bp
ATGGAGATGATAGCGGAACGATCCCTAGCGGCGATGCAGGGTAAGTATCTCTACATATGTACCAGCAAAACGCACAAATTTTTTCCCGATCGGTGGGTTGTTCACCGAATCGAAATGAGCGATGTGTTATCTGCATCGGAAGTCCATATTCGTTATCTGCCTTTCAAGCAAGTGGCTAATGAAATTGGCGACCGTGACCTTCCGTATATGGTGGGTTGTGGTGTCTTCAAATCCAAAATTCTCATCGGCGGCGGCCTGAAGAAGAGACACAACGGATCAGAAACTCAAGTTGGAGAGCTTTTCCCCTTTCCGAGGAGGGAAAGCTCAACCCTGCTAGTAGAGATGAAGGGGCAACTTTATGCTCTTGCCGGTGACCTGATCAAGTTGAGATACGATAGTCCTTTTGAGAGATTTGACCCGTCCTGCAAAAAATGGTCACCCCTGCCCGGACCTCCTCTTTGGGATCCTGACTCTCCATTCGATAGGACTCCCCTCGTCGGTTCAGGTTATGTAATTACAGACACCAGCATTCTTTCCTCACTTGAGGCTACCCCGGTGTTCCGTTTTGACATCACGAAGCCGAATGCAGGATGGTCTGTACAGGAATCTTTCTTTTCCCTTTCAAGGCACAACTCTAGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

24.17

Weight (kDa)

8.38

Isoelectric Point (pI)

63.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 14, 29, 249, 252
AclWI GGATC 4 cut(s) 15, 283, 458, 471
AcsI RAATTY 2 cut(s) 77, 237
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 2 cut(s) 61, 600
AfiI CCNNNNNNNGG 6 cut(s) 93, 312, 446, 497, 587, 624
AgsI TTSAA 3 cut(s) 166, 229, 624
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 2 cut(s) 298, 324
AluI AGCT 2 cut(s) 298, 324
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 4 cut(s) 15, 283, 458, 471
AoxI GGCC 1 cut(s) 253
ApoI RAATTY 2 cut(s) 77, 237
ArsI GACNNNNNNTTYG 4 cut(s) 441, 461, 473, 493
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 2 cut(s) 447, 554
AsuHPI GGTGA 3 cut(s) 95, 383, 429
AvaII GGWCC 1 cut(s) 449
BaeI ACNNNNGTAYC 4 cut(s) 43, 76, 382, 415
BamHI GGATCC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 217
BccI CCATC 1 cut(s) 585
BclI TGATCA 1 cut(s) 378
BcnI CCSGG 2 cut(s) 447, 554
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 3 cut(s) 26, 335, 636
BisI GCNGC 3 cut(s) 30, 250, 253
BlsI GCNGC 3 cut(s) 31, 251, 254
Bme1390I CCNGG 2 cut(s) 447, 554
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 2 cut(s) 447, 554
BmsI GCATC 2 cut(s) 24, 143
BpiI GAAGAC 1 cut(s) 217
BpuEI CTTGAG 2 cut(s) 270, 563
BpuMI CCSGG 2 cut(s) 447, 554
BsaJI CCNNGG 2 cut(s) 311, 552
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 312, 446, 497, 587, 624
Bse118I RCCGGY 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 311, 552
BseGI GGATG 1 cut(s) 596
BseLI CCNNNNNNNGG 6 cut(s) 93, 312, 446, 497, 587, 624
BseRI GAGGAG 2 cut(s) 328, 444
Bsh1285I CGRYCG 2 cut(s) 91, 193
BshFI GGCC 1 cut(s) 255
BsiEI CGRYCG 2 cut(s) 91, 193
BsiSI CCGG 3 cut(s) 369, 447, 554
BslI CCNNNNNNNGG 6 cut(s) 93, 312, 446, 497, 587, 624
BsmAI GTCTC 1 cut(s) 259
BsmI GAATGC 2 cut(s) 528, 589
BsnI GGCC 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 5 cut(s) 20, 88, 275, 378, 463
BspACI CCGC 4 cut(s) 14, 29, 249, 252
BspANI GGCC 1 cut(s) 255
BspHI TCATGA 1 cut(s) 641
BspLI GGNNCC 1 cut(s) 465
BspPI GGATC 4 cut(s) 15, 283, 458, 471
BsrFI RCCGGY 1 cut(s) 368
BsrGI TGTACA 1 cut(s) 598
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 2 cut(s) 311, 552
BssMI GATC 5 cut(s) 20, 88, 275, 378, 463
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 598
BstEII GGTNACC 2 cut(s) 371, 435
BstENI CCTNNNNNAGG 1 cut(s) 310
BstF5I GGATG 1 cut(s) 596
BstKTI GATC 5 cut(s) 23, 91, 278, 381, 466
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 5 cut(s) 20, 88, 275, 378, 463
BstMCI CGRYCG 2 cut(s) 91, 193
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstPI GGTNACC 2 cut(s) 371, 435
BstSCI CCNGG 2 cut(s) 445, 552
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
BsuRI GGCC 1 cut(s) 255
BtgZI GCGATG 2 cut(s) 47, 135
BtsCI GGATG 1 cut(s) 596
CciI TCATGA 1 cut(s) 641
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 60, 599
CviAII CATG 1 cut(s) 642
CviJI RGCY 6 cut(s) 176, 255, 298, 324, 548, 580
CviKI_1 RGCY 6 cut(s) 176, 255, 298, 324, 548, 580
CviQI GTAC 2 cut(s) 60, 599
DpnI GATC 5 cut(s) 22, 90, 277, 380, 465
DpnII GATC 5 cut(s) 20, 88, 275, 378, 463
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 278
Eco91I GGTNACC 2 cut(s) 371, 435
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoO65I GGTNACC 2 cut(s) 371, 435
FaeI CATG 1 cut(s) 645
FaiI YATR 8 cut(s) 56, 58, 147, 207, 209, 360, 510, 643
FatI CATG 1 cut(s) 641
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 3 cut(s) 30, 250, 253
FokI GGATG 1 cut(s) 603
Fsp4HI GCNGC 3 cut(s) 30, 250, 253
FspBI CTAG 3 cut(s) 26, 335, 636
GluI GCNGC 3 cut(s) 30, 250, 253
HaeIII GGCC 1 cut(s) 255
HapII CCGG 3 cut(s) 369, 447, 554
Hin1II CATG 1 cut(s) 645
HinfI GANTC 4 cut(s) 108, 470, 487, 605
HpaII CCGG 3 cut(s) 369, 447, 554
HphI GGTGA 3 cut(s) 95, 383, 429
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 3 cut(s) 139, 280, 312
Hpy188III TCNNGA 4 cut(s) 86, 467, 574, 642
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 2 cut(s) 209, 340
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4V TGCA 4 cut(s) 37, 134, 426, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
Hsp92II CATG 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 5 cut(s) 20, 88, 275, 378, 463
LweI GCATC 2 cut(s) 24, 143
MaeI CTAG 3 cut(s) 26, 335, 636
MaeIII GTNAC 3 cut(s) 194, 371, 435
MalI GATC 5 cut(s) 22, 90, 277, 380, 465
MboI GATC 5 cut(s) 20, 88, 275, 378, 463
MboII GAAGA 3 cut(s) 217, 271, 274
MflI RGATCY 1 cut(s) 463
MluCI AATT 4 cut(s) 77, 183, 237, 513
MlyI GAGTC 2 cut(s) 464, 481
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 7 cut(s) 306, 309, 462, 465, 503, 538, 547
MspI CCGG 3 cut(s) 369, 447, 554
MspR9I CCNGG 2 cut(s) 447, 554
Mva1269I GAATGC 2 cut(s) 528, 589
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 2 cut(s) 447, 554
NdeI CATATG 1 cut(s) 56
NdeII GATC 5 cut(s) 20, 88, 275, 378, 463
NlaIII CATG 1 cut(s) 645
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 3 cut(s) 194, 371, 435
PagI TCATGA 1 cut(s) 641
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 2 cut(s) 528, 589
PfeI GAWTC 2 cut(s) 108, 605
PkrI GCNGC 3 cut(s) 31, 251, 254
Ple19I CGATCG 1 cut(s) 91
PleI GAGTC 2 cut(s) 464, 481
PpsI GAGTC 2 cut(s) 464, 481
PspEI GGTNACC 2 cut(s) 371, 435
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 449
PsuI RGATCY 1 cut(s) 463
PvuI CGATCG 1 cut(s) 91
RsaI GTAC 2 cut(s) 61, 600
RsaNI GTAC 2 cut(s) 60, 599
SatI GCNGC 3 cut(s) 30, 250, 253
Sau3AI GATC 5 cut(s) 20, 88, 275, 378, 463
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 2 cut(s) 464, 481
ScrFI CCNGG 2 cut(s) 447, 554
SetI ASST 6 cut(s) 201, 300, 326, 378, 454, 508
SfaNI GCATC 2 cut(s) 24, 143
SinI GGWCC 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 285, 542
SmoI CTYRAG 2 cut(s) 285, 542
Sse9I AATT 4 cut(s) 77, 183, 237, 513
SsiI CCGC 4 cut(s) 14, 29, 249, 252
SspMI CTAG 3 cut(s) 26, 335, 636
StyD4I CCNGG 2 cut(s) 445, 552
TaaI ACNGT 1 cut(s) 194
TaqI TCGA 2 cut(s) 111, 480
TasI AATT 4 cut(s) 77, 183, 237, 513
TatI WGTACW 1 cut(s) 598
TauI GCSGC 3 cut(s) 32, 252, 255
TfiI GAWTC 2 cut(s) 108, 605
TseFI GTSAC 3 cut(s) 194, 371, 435
Tsp45I GTSAC 3 cut(s) 194, 371, 435
TspDTI ATGAA 3 cut(s) 195, 359, 630
TspGWI ACGGA 3 cut(s) 192, 288, 551
VpaK11BI GGWCC 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 310
XapI RAATTY 2 cut(s) 77, 237
XspI CTAG 3 cut(s) 26, 335, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.