Rh6BG440100

Nuclear pore complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015152
Physical Location & Seq
Forward (+)
65825801 .. 65833173
7373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG440100.1

Sequence Viewer

Length: 1980 bp
ATGCCCGGCGTCGCATCAAACTCCGGCGAACGTTCTCTGGTCCCCTACTCGCCGGAGCTCCCGCATCCCGTCGTACACTCTATTCACCACGGCCTCAAGCCTCCGATCGCTCGCCTCTCCATCTCGTGGTCCCGCGGCAATACGCTGCGCGTGTCGGTTCTCCGTCCGCCGTCCGATGAAGACTCCGACGACGGCGAGGTCGGCGGGAAAGTAGTGGAGGTGAAGCTCAGCAATGGCGACGGCGAGAATAGCGACGCAACGTGGCGGAGGATCGCCTACGGCTCCGTTACCCCGTTCGCTCATCTCCAGAGCCGCCGGAGCACCATCGCCAACTTATCGAACATGTCACTCAGTCCATCGCCGTATAGCATCGATTGGTGGGAGTATGTGATGGAGTACAGCAAGGACATAAATTCACTCCTTGGTAGTCTCGATTCGGGTCCTGATCCGGTGATTGACGACCCGAAGATGGTTTTAAAGAGAACTGAGGAGCCTACCTCGATAAAAGCTGCTTGGGAGCTGATGGAGATATTTTATGCGGACAAGCAGTCTCAGCCATGGCAACTGGAACGGCTTGTTGATTGGTTGGCTGATTATGATAGCTTGCTCTCGAGTACTCATCCGACAGTCCATTCTAAACTTGTGGATTTCCAAAATGAACTTTCCAGCTTACAGGTTATTGAGGATGATCCTAAATATTGGGATGTGTTATCATCAGCACTCGCAGTTGGCTGGATTGATATTGTGGTGAAAATGTTACGCTTACATGGATCTTATCAACTCCATCAGCTTGGCAACCGTGAGACAGAAAATGGACTTGTAGAGGCAGTTGCTGTTCTTATTTCAACGAAGCCACGCATGCGTCCAGGAGTAGAAGATGGAAAGTTGGGTGAATGCTTTAAAGCAAAGCCTGATTTTATCAAGGCATGGGAGAATTGGAGAGCAAAAATTACGAAGCTAGACTGCAGTGGATTCTGGCTTCAGTGTGATCACCATCAAACCCGGGAGGGATTGAAAAATATGATACAGGTTATGCTTGGAAACATTGACAGTCTCTGCAATGAAACTTGCCACTGGATAGAGCTGTTCATCTCTCACTTTCTATACGTCAGGCCATTAACAGTGGGTCTCGAAAGCATGTATGGTCTAGCCCAGAAATGCTTACAATTGAAACCAATGTTGGGTGCCCATAGGTTGATAGATATTATTCTTGGAATCCTTGGGGAAAATACTGAGGTTGTCTTGGCGGAATGCTCTAGAGCATTTGGGCCTTGGATGGTTGCCCATGCCATTGAGTTGTTGACTACTGGGAGTGATCACGCAGAAAGTCTTCTACATGAAGAGCGCTATAACTTGGGTGGAATTAGTATAGCAGAACTACATCGGCTTGTCTACGCTCAGGTTTTATCATCACATGCATTGACTTGGCAAATTGCTCCAATCTATTTGTCATCGTGCATGAAGCAGGGAATGGGTTTGTTAGAGATTCTATTGTCTAAGCTGCCTATTGAACATAATGAAACACTGCTTAAGAATATAGAGATCTGTCGTCTTTACGAACTTGATACTGTTAGTTCAAACGTTATGAAGATTGCAGGGGTTTACCATTGGAAGCATGGTAGGAAAGGTTGTGGAGTTTTCTGGCTTCAGCAAGCCCGAGATGAAGTTCGCCTTACCAGGATTGCTCAACATTTGTTTGATTCTGTTGGAAAGTCGATCTCCGATGAGAGTTTCAAGCAATGGGAAGGCTTGATTGAATTGTTGGGGTCTGAGTCCAAGACTACTGGGGGTCTAGACTTCCTGCACAAGTATAGGGATTTCAAGAAGTCCCTCAAGCAGGTTCGTGATGGAAAAACTACTGATGCTGCCCATCAAGCTGTAGAATCTCTGATATCGCTCATGAAAAACCCATCTACTCCTCAGCGCTTCTGGCTGCCTCTTCTTCATGACTCAGTAAGTTCATTAAAATATTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nucleopor_Nup85

Protein Analysis

659

Amino Acids

74.22

Weight (kDa)

6.01

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleopor_Nup85 PF07575 81 - 656 5.3e-135 Nup85 Nucleoporin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015152)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32910
fragaria_vesca FvH4_2g35520
malus_domestica MD15G1093200.v1.1
prunus_persica Prupe.1G448000_v2.0.a1
pyrus_communis pycom15g08600
rosa_chinensis RchiOBHm_Chr6g0306261
rosa_laevigata RLG00000010809
rosa_multiflora Rmu_sc0017002.1_g000002 Rmu_sc0037804.1_g000002
rosa_roxburghii Rroxscaffold_7G00161900
rosa_rugosa Rorug06G0348400
rosa_samantha Rh6AG460900 Rh6BG440100 Rh6CG475200 Rh6DG461500
rosa_wichuraiana Rw6G040160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 1828
AccB1I GGYRCC 1 cut(s) 1184
AccB7I CCANNNNNTGG 1 cut(s) 126
AccI GTMKAC 1 cut(s) 1392
AccII CGCG 2 cut(s) 135, 150
AciI CCGC 9 cut(s) 62, 133, 135, 167, 204, 265, 313, 539, 1247
AclI AACGTT 2 cut(s) 31, 1581
AclWI GGATC 4 cut(s) 278, 440, 683, 778
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 2 cut(s) 965, 1631
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 3 cut(s) 75, 398, 616
AfeI AGCGCT 2 cut(s) 1346, 1925
AfiI CCNNNNNNNGG 4 cut(s) 126, 469, 1181, 1837
AflII CTTAAG 1 cut(s) 1529
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 8 cut(s) 846, 1015, 1171, 1511, 1578, 1735, 1757, 1822
AhdI GACNNNNNGTC 1 cut(s) 547
AjnI CCWGG 2 cut(s) 865, 1676
AjuI GAANNNNNNNTTGG 2 cut(s) 1163, 1195
Alw21I GWGCWC 2 cut(s) 60, 323
Alw26I GTCTC 5 cut(s) 434, 555, 797, 1058, 1133
AlwI GGATC 4 cut(s) 278, 440, 683, 778
AlwNI CAGNNNCTG 2 cut(s) 833, 1056
Ama87I CYCGRG 3 cut(s) 610, 1002, 1656
Aor51HI AGCGCT 2 cut(s) 1346, 1925
AoxI GGCC 3 cut(s) 91, 1112, 1268
ApeKI GCWGC 5 cut(s) 145, 509, 1501, 1865, 1933
ApoI RAATTY 1 cut(s) 412
Asp700I GAANNNNTTC 1 cut(s) 1329
AspLEI GCGC 3 cut(s) 150, 1347, 1926
AspS9I GGNCC 4 cut(s) 40, 129, 440, 1268
AsuC2I CCSGG 3 cut(s) 6, 1003, 1004
AsuHPI GGTGA 6 cut(s) 77, 232, 463, 760, 902, 983
AvaI CYCGRG 3 cut(s) 610, 1002, 1656
AvaII GGWCC 3 cut(s) 40, 129, 440
BaeGI GKGCMC 1 cut(s) 1189
BanI GGYRCC 1 cut(s) 1184
BanII GRGCYC 1 cut(s) 60
BauI CACGAG 1 cut(s) 124
BbsI GAAGAC 2 cut(s) 186, 1322
Bbv12I GWGCWC 2 cut(s) 60, 323
BbvCI CCTCAGC 1 cut(s) 1920
BbvI GCAGC 5 cut(s) 132, 496, 1488, 1852, 1920
BceAI ACGGC 7 cut(s) 106, 154, 208, 256, 295, 346, 587
BciT130I CCWGG 2 cut(s) 867, 1678
BclI TGATCA 2 cut(s) 988, 1315
BcnI CCSGG 3 cut(s) 6, 1003, 1004
BcoDI GTCTC 5 cut(s) 434, 555, 797, 1058, 1133
BfaI CTAG 4 cut(s) 959, 1148, 1257, 1793
BfmI CTRYAG 2 cut(s) 964, 1878
BfoI RGCGCY 2 cut(s) 1348, 1927
BfrI CTTAAG 1 cut(s) 1529
BfuAI ACCTGC 1 cut(s) 1828
BglII AGATCT 1 cut(s) 1542
BisI GCNGC 7 cut(s) 136, 146, 313, 510, 1502, 1866, 1934
BlpI GCTNAGC 1 cut(s) 227
BlsI GCNGC 7 cut(s) 137, 147, 314, 511, 1503, 1867, 1935
BmcAI AGTACT 1 cut(s) 616
Bme1390I CCNGG 5 cut(s) 6, 867, 1003, 1004, 1678
Bme18I GGWCC 3 cut(s) 40, 129, 440
BmeRI GACNNNNNGTC 1 cut(s) 547
BmeT110I CYCGRG 3 cut(s) 610, 1002, 1656
BmgT120I GGNCC 4 cut(s) 40, 129, 440, 1268
BmiI GGNNCC 6 cut(s) 42, 131, 283, 441, 492, 1186
BmrFI CCNGG 5 cut(s) 6, 867, 1003, 1004, 1678
BmrI ACTGGG 2 cut(s) 1317, 1794
BmsI GCATC 4 cut(s) 23, 73, 378, 1852
BmuI ACTGGG 2 cut(s) 1317, 1794
BpiI GAAGAC 2 cut(s) 186, 1322
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpmI CTGGAG 1 cut(s) 290
Bpu10I CCTNAGC 2 cut(s) 1398, 1920
Bpu1102I GCTNAGC 1 cut(s) 227
BpuEI CTTGAG 2 cut(s) 80, 1817
BpuMI CCSGG 3 cut(s) 6, 1003, 1004
Bsa29I ATCGAT 1 cut(s) 372
BsaBI GATNNNNATC 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 1133
BsaJI CCNNGG 7 cut(s) 88, 133, 421, 557, 1002, 1219, 1271
BsaWI WCCGGW 1 cut(s) 448
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 469, 1181, 1837
Bse1I ACTGG 4 cut(s) 570, 1079, 1312, 1789
Bse3DI GCAATG 3 cut(s) 238, 1066, 1745
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 2 cut(s) 867, 1678
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 7 cut(s) 88, 133, 421, 557, 1002, 1219, 1271
BseGI GGATG 5 cut(s) 64, 619, 691, 709, 1281
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 4 cut(s) 126, 469, 1181, 1837
BseMI GCAATG 3 cut(s) 238, 1066, 1745
BseMII CTCAG 9 cut(s) 241, 364, 477, 566, 1224, 1412, 1761, 1934, 1965
BseNI ACTGG 4 cut(s) 570, 1079, 1312, 1789
BseRI GAGGAG 2 cut(s) 503, 1908
BseSI GKGCMC 1 cut(s) 1189
BseXI GCAGC 5 cut(s) 132, 496, 1488, 1852, 1920
BsgI GTGCAG 1 cut(s) 1787
Bsh1236I CGCG 2 cut(s) 135, 150
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 93, 1114, 1270
BshNI GGYRCC 1 cut(s) 1184
BshVI ATCGAT 1 cut(s) 372
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 60, 323
BsiHKCI CYCGRG 3 cut(s) 610, 1002, 1656
BsiSI CCGG 6 cut(s) 6, 24, 53, 316, 449, 1003
BslFI GGGAC 3 cut(s) 26, 115, 1813
BslI CCNNNNNNNGG 4 cut(s) 126, 469, 1181, 1837
BsmAI GTCTC 5 cut(s) 434, 555, 797, 1058, 1133
BsmFI GGGAC 3 cut(s) 26, 115, 1813
BsmI GAATGC 2 cut(s) 899, 1256
BsnI GGCC 3 cut(s) 93, 1114, 1270
Bso31I GGTCTC 1 cut(s) 1133
BsoBI CYCGRG 3 cut(s) 610, 1002, 1656
Bsp1286I GDGCHC 3 cut(s) 60, 323, 1189
Bsp143I GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
Bsp1720I GCTNAGC 1 cut(s) 227
Bsp19I CCATGG 1 cut(s) 557
BspACI CCGC 9 cut(s) 62, 133, 135, 167, 204, 265, 313, 539, 1247
BspANI GGCC 3 cut(s) 93, 1114, 1270
BspCNI CTCAG 9 cut(s) 240, 363, 478, 565, 1225, 1411, 1762, 1933, 1964
BspDI ATCGAT 1 cut(s) 372
BspFNI CGCG 2 cut(s) 135, 150
BspHI TCATGA 2 cut(s) 1899, 1945
BspLI GGNNCC 6 cut(s) 42, 131, 283, 441, 492, 1186
BspMAI CTGCAG 1 cut(s) 968
BspMI ACCTGC 1 cut(s) 1828
BspPI GGATC 4 cut(s) 278, 440, 683, 778
BspQI GCTCTTC 1 cut(s) 1335
BspT107I GGYRCC 1 cut(s) 1184
BspTI CTTAAG 1 cut(s) 1529
BspTNI GGTCTC 1 cut(s) 1133
BsrDI GCAATG 3 cut(s) 238, 1066, 1745
BsrI ACTGG 4 cut(s) 570, 1079, 1312, 1789
BssECI CCNNGG 7 cut(s) 88, 133, 421, 557, 1002, 1219, 1271
BssMI GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
BssNI GRCGYC 1 cut(s) 9
BssSI CACGAG 1 cut(s) 124
BssT1I CCWWGG 4 cut(s) 421, 557, 1219, 1271
Bst2BI CACGAG 1 cut(s) 124
Bst2UI CCWGG 2 cut(s) 867, 1678
Bst4CI ACNGT 5 cut(s) 628, 800, 1052, 1123, 1570
Bst6I CTCTTC 2 cut(s) 1335, 1944
BstACI GRCGYC 1 cut(s) 9
BstAFI CTTAAG 1 cut(s) 1529
BstC8I GCNNGC 4 cut(s) 112, 605, 860, 1653
BstDSI CCRYGG 3 cut(s) 88, 133, 557
BstENI CCTNNNNNAGG 1 cut(s) 1835
BstF5I GGATG 5 cut(s) 64, 619, 691, 709, 1281
BstFNI CGCG 2 cut(s) 135, 150
BstH2I RGCGCY 2 cut(s) 1348, 1927
BstHHI GCGC 3 cut(s) 150, 1347, 1926
BstKTI GATC 9 cut(s) 108, 273, 448, 691, 773, 991, 1318, 1545, 1719
BstMAI GTCTC 5 cut(s) 434, 555, 797, 1058, 1133
BstMBI GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 7 cut(s) 201, 249, 318, 553, 859, 1874, 1930
BstNI CCWGG 2 cut(s) 867, 1678
BstNSI RCATGY 4 cut(s) 346, 862, 1141, 1418
BstSCI CCNGG 5 cut(s) 4, 865, 1001, 1002, 1676
BstSFI CTRYAG 2 cut(s) 964, 1878
BstSLI GKGCMC 1 cut(s) 1189
BstUI CGCG 2 cut(s) 135, 150
BstV1I GCAGC 5 cut(s) 132, 496, 1488, 1852, 1920
BstV2I GAAGAC 2 cut(s) 186, 1322
BstX2I RGATCY 2 cut(s) 770, 1542
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 2 cut(s) 770, 1542
Bsu15I ATCGAT 1 cut(s) 372
BsuRI GGCC 3 cut(s) 93, 1114, 1270
BsuTUI ATCGAT 1 cut(s) 372
BtgI CCRYGG 3 cut(s) 88, 133, 557
BtgZI GCGATG 2 cut(s) 310, 342
BtsCI GGATG 5 cut(s) 64, 619, 691, 709, 1281
BtsI GCAGTG 2 cut(s) 973, 1523
BtsIMutI CAGTG 5 cut(s) 973, 989, 1072, 1128, 1523
BveI ACCTGC 1 cut(s) 1828
Cac8I GCNNGC 4 cut(s) 112, 605, 860, 1653
CaiI CAGNNNCTG 2 cut(s) 833, 1056
CciI TCATGA 2 cut(s) 1899, 1945
CfoI GCGC 3 cut(s) 150, 1347, 1926
Cfr13I GGNCC 4 cut(s) 40, 129, 440, 1268
Cfr42I CCGCGG 1 cut(s) 136
Cfr9I CCCGGG 1 cut(s) 1002
ClaI ATCGAT 1 cut(s) 372
CseI GACGC 2 cut(s) 263, 851
Csp6I GTAC 3 cut(s) 74, 397, 615
CviQI GTAC 3 cut(s) 74, 397, 615
DpnI GATC 9 cut(s) 107, 272, 447, 690, 772, 990, 1317, 1544, 1718
DpnII GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
DraI TTTAAA 2 cut(s) 477, 901
DrdI GACNNNNNNGTC 1 cut(s) 197
DriI GACNNNNNGTC 1 cut(s) 547
DseDI GACNNNNNNGTC 1 cut(s) 197
Eam1104I CTCTTC 2 cut(s) 1335, 1944
Eam1105I GACNNNNNGTC 1 cut(s) 547
EarI CTCTTC 2 cut(s) 1335, 1944
EciI GGCGGA 3 cut(s) 156, 280, 1262
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 4 cut(s) 421, 557, 1219, 1271
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 1133
Eco32I GATATC 1 cut(s) 1893
Eco47I GGWCC 3 cut(s) 40, 129, 440
Eco47III AGCGCT 2 cut(s) 1346, 1925
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 2 cut(s) 965, 1631
Eco88I CYCGRG 3 cut(s) 610, 1002, 1656
EcoICRI GAGCTC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 1835
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 865, 1676
EcoRV GATATC 1 cut(s) 1893
EcoT14I CCWWGG 4 cut(s) 421, 557, 1219, 1271
EcoT22I ATGCAT 1 cut(s) 1420
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 4 cut(s) 421, 557, 1219, 1271
FalI AAGNNNNNCTT 2 cut(s) 1656, 1688
FaqI GGGAC 3 cut(s) 26, 115, 1813
FauI CCCGC 3 cut(s) 69, 140, 197
FbaI TGATCA 2 cut(s) 988, 1315
FblI GTMKAC 1 cut(s) 1392
Fnu4HI GCNGC 7 cut(s) 136, 146, 313, 510, 1502, 1866, 1934
FokI GGATG 5 cut(s) 51, 606, 698, 716, 1288
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 7 cut(s) 136, 146, 313, 510, 1502, 1866, 1934
FspBI CTAG 4 cut(s) 959, 1148, 1257, 1793
GlaI GCGC 3 cut(s) 149, 1346, 1925
GluI GCNGC 7 cut(s) 136, 146, 313, 510, 1502, 1866, 1934
GsuI CTGGAG 1 cut(s) 290
HaeII RGCGCY 2 cut(s) 1348, 1927
HaeIII GGCC 3 cut(s) 93, 1114, 1270
HapII CCGG 6 cut(s) 6, 24, 53, 316, 449, 1003
HgaI GACGC 2 cut(s) 263, 851
HhaI GCGC 3 cut(s) 150, 1347, 1926
Hin1I GRCGYC 1 cut(s) 9
Hin6I GCGC 3 cut(s) 148, 1345, 1924
HinP1I GCGC 3 cut(s) 148, 1345, 1924
HincII GTYRAC 1 cut(s) 1302
HindII GTYRAC 1 cut(s) 1302
HinfI GANTC 9 cut(s) 182, 434, 972, 1215, 1486, 1700, 1772, 1883, 1949
HpaII CCGG 6 cut(s) 6, 24, 53, 316, 449, 1003
HphI GGTGA 6 cut(s) 77, 232, 463, 760, 902, 983
Hpy166II GTNNAC 4 cut(s) 76, 1302, 1393, 1603
Hpy188I TCNGA 7 cut(s) 105, 175, 187, 624, 1723, 1771, 1890
Hpy8I GTNNAC 4 cut(s) 76, 1302, 1393, 1603
Hpy99I CGWCG 6 cut(s) 14, 74, 191, 194, 242, 257
HpyAV CCTTC 1 cut(s) 1739
HpyCH4III ACNGT 5 cut(s) 628, 800, 1052, 1123, 1570
HpyCH4IV ACGT 4 cut(s) 31, 260, 1107, 1581
HpyCH4V TGCA 6 cut(s) 966, 1059, 1418, 1458, 1595, 1804
HpyF10VI GCNNNNNNNGC 7 cut(s) 201, 249, 318, 553, 859, 1874, 1930
HpySE526I ACGT 4 cut(s) 31, 260, 1107, 1581
Hsp92I GRCGYC 1 cut(s) 9
HspAI GCGC 3 cut(s) 148, 1345, 1924
Ksp22I TGATCA 2 cut(s) 988, 1315
KspI CCGCGG 1 cut(s) 136
Kzo9I GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 7 cut(s) 55, 63, 287, 318, 490, 517, 1441
Lsp1109I GCAGC 5 cut(s) 132, 496, 1488, 1852, 1920
LweI GCATC 4 cut(s) 23, 73, 378, 1852
MaeI CTAG 4 cut(s) 959, 1148, 1257, 1793
MaeII ACGT 4 cut(s) 31, 260, 1107, 1581
MaeIII GTNAC 3 cut(s) 286, 345, 756
MalI GATC 9 cut(s) 107, 272, 447, 690, 772, 990, 1317, 1544, 1718
MboI GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
MboII GAAGA 8 cut(s) 191, 478, 887, 1322, 1352, 1600, 1931, 1934
MfeI CAATTG 1 cut(s) 1166
MflI RGATCY 2 cut(s) 770, 1542
MhlI GDGCHC 3 cut(s) 60, 323, 1189
MluCI AATT 7 cut(s) 412, 934, 948, 1166, 1362, 1431, 1757
MlyI GAGTC 3 cut(s) 176, 1781, 1943
MmeI TCCRAC 3 cut(s) 210, 647, 1687
Mph1103I ATGCAT 1 cut(s) 1420
MroXI GAANNNNTTC 1 cut(s) 1329
MseI TTAA 6 cut(s) 476, 900, 1118, 1530, 1964, 1978
MspA1I CMGCKG 1 cut(s) 135
MspCI CTTAAG 1 cut(s) 1529
MspI CCGG 6 cut(s) 6, 24, 53, 316, 449, 1003
MspR9I CCNGG 5 cut(s) 6, 867, 1003, 1004, 1678
MunI CAATTG 1 cut(s) 1166
Mva1269I GAATGC 2 cut(s) 899, 1256
MvaI CCWGG 2 cut(s) 867, 1678
MvnI CGCG 2 cut(s) 135, 150
MwoI GCNNNNNNNGC 7 cut(s) 201, 249, 318, 553, 859, 1874, 1930
NciI CCSGG 3 cut(s) 6, 1003, 1004
NcoI CCATGG 1 cut(s) 557
NdeII GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
NlaIV GGNNCC 6 cut(s) 42, 131, 283, 441, 492, 1186
NmuCI GTSAC 1 cut(s) 345
NsiI ATGCAT 1 cut(s) 1420
NspI RCATGY 4 cut(s) 346, 862, 1141, 1418
PaeI GCATGC 1 cut(s) 862
PaeR7I CTCGAG 1 cut(s) 610
PagI TCATGA 2 cut(s) 1899, 1945
PciI ACATGT 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 1335
PctI GAATGC 2 cut(s) 899, 1256
PdmI GAANNNNTTC 1 cut(s) 1329
PfeI GAWTC 6 cut(s) 434, 972, 1215, 1486, 1700, 1883
PflMI CCANNNNNTGG 1 cut(s) 126
PfoI TCCNGGA 1 cut(s) 865
PkrI GCNGC 7 cut(s) 137, 147, 314, 511, 1503, 1867, 1935
Ple19I CGATCG 1 cut(s) 108
PleI GAGTC 3 cut(s) 176, 1780, 1943
PpsI GAGTC 3 cut(s) 176, 1780, 1943
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 60
Psp1406I AACGTT 2 cut(s) 31, 1581
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 865, 1676
PspGI CCWGG 2 cut(s) 865, 1676
PspN4I GGNNCC 6 cut(s) 42, 131, 283, 441, 492, 1186
PspPI GGNCC 4 cut(s) 40, 129, 440, 1268
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 1 cut(s) 968
PstNI CAGNNNCTG 2 cut(s) 833, 1056
PsuI RGATCY 2 cut(s) 770, 1542
PvuI CGATCG 1 cut(s) 108
RsaI GTAC 3 cut(s) 75, 398, 616
RsaNI GTAC 3 cut(s) 74, 397, 615
SacI GAGCTC 1 cut(s) 60
SacII CCGCGG 1 cut(s) 136
SapI GCTCTTC 1 cut(s) 1335
SaqAI TTAA 6 cut(s) 476, 900, 1118, 1530, 1964, 1978
SatI GCNGC 7 cut(s) 136, 146, 313, 510, 1502, 1866, 1934
Sau3AI GATC 9 cut(s) 105, 270, 445, 688, 770, 988, 1315, 1542, 1716
Sau96I GGNCC 4 cut(s) 40, 129, 440, 1268
ScaI AGTACT 1 cut(s) 616
SchI GAGTC 3 cut(s) 176, 1781, 1943
ScrFI CCNGG 5 cut(s) 6, 867, 1003, 1004, 1678
SduI GDGCHC 3 cut(s) 60, 323, 1189
SfaNI GCATC 4 cut(s) 23, 73, 378, 1852
SfcI CTRYAG 2 cut(s) 964, 1878
Sfr274I CTCGAG 1 cut(s) 610
Sfr303I CCGCGG 1 cut(s) 136
SgrBI CCGCGG 1 cut(s) 136
SinI GGWCC 3 cut(s) 40, 129, 440
SlaI CTCGAG 1 cut(s) 610
SmaI CCCGGG 1 cut(s) 1004
SmlI CTYRAG 4 cut(s) 95, 610, 1529, 1832
SmoI CTYRAG 4 cut(s) 95, 610, 1529, 1832
SphI GCATGC 1 cut(s) 862
Sse9I AATT 7 cut(s) 412, 934, 948, 1166, 1362, 1431, 1757
SsiI CCGC 9 cut(s) 62, 133, 135, 167, 204, 265, 313, 539, 1247
SspI AATATT 2 cut(s) 698, 1970
SspMI CTAG 4 cut(s) 959, 1148, 1257, 1793
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 5 cut(s) 4, 865, 1001, 1002, 1676
StyI CCWWGG 4 cut(s) 421, 557, 1219, 1271
TaaI ACNGT 5 cut(s) 628, 800, 1052, 1123, 1570
TaiI ACGT 4 cut(s) 34, 263, 1110, 1584
TaqI TCGA 7 cut(s) 338, 372, 432, 500, 611, 1131, 1715
TasI AATT 7 cut(s) 412, 934, 948, 1166, 1362, 1431, 1757
TatI WGTACW 2 cut(s) 396, 614
TauI GCSGC 2 cut(s) 138, 315
TfiI GAWTC 6 cut(s) 434, 972, 1215, 1486, 1700, 1883
Tru1I TTAA 6 cut(s) 476, 900, 1118, 1530, 1964, 1978
Tru9I TTAA 6 cut(s) 476, 900, 1118, 1530, 1964, 1978
TscAI CASTG 5 cut(s) 973, 989, 1079, 1128, 1530
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 5 cut(s) 145, 509, 1501, 1865, 1933
Tsp45I GTSAC 1 cut(s) 345
TspGWI ACGGA 2 cut(s) 152, 274
TspMI CCCGGG 1 cut(s) 1002
TspRI CASTG 5 cut(s) 973, 989, 1079, 1128, 1530
Van91I CCANNNNNTGG 1 cut(s) 126
Vha464I CTTAAG 1 cut(s) 1529
VpaK11BI GGWCC 3 cut(s) 40, 129, 440
XagI CCTNNNNNAGG 1 cut(s) 1835
XapI RAATTY 1 cut(s) 412
XbaI TCTAGA 2 cut(s) 1256, 1792
XceI RCATGY 4 cut(s) 346, 862, 1141, 1418
XcmI CCANNNNNNNNNTGG 1 cut(s) 1613
XhoI CTCGAG 1 cut(s) 610
XmaI CCCGGG 1 cut(s) 1002
XmiI GTMKAC 1 cut(s) 1392
XmnI GAANNNNTTC 1 cut(s) 1329
XspI CTAG 4 cut(s) 959, 1148, 1257, 1793
ZrmI AGTACT 1 cut(s) 616
Zsp2I ATGCAT 1 cut(s) 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.