Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0004418

Physical Location & Seq

Genomic Coordinates Forward (+)

3954687 .. 3957997

Gene Structure 3311 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG041000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGGCTTATCTTGGCATTTTTGTGCTCTTGTTATTGCTTAGTTGTAGGTTCAGCCATGGTGTTGAGAGTGATATAAACTGCTTGAGAAGTATAAAAGCAACATTGGAGGACCCTTTAGACATGTTAACCTCTTCATGGGATTTTAACAATGCCACCGAAGGATTCATCTGTAACTTTCTGGGAATCGAGTGTTGGCACCCTGATGAGAGCAGAGTTTTAAACATCAAGCTTGCGGATTTGGGACTCAAAGGCCACTTTCCTCGTGGTGTAGCGAATTGTACAAGCTTAACAGGCTTAGATCTTTCAAGCAACAACCTCAGTGGAGCTATTCCTGATGATATTGGTCGTTTAATCCCTTTCATTACGTCGCTTGAGCTCTCGTCCAACAGCTTCTCAGGGCTAATTCCCAGGAATATCTCCAATTGTACTTATCTGAATGTCCTTAAACTTGACAACAACAAGTTAACGGGTCAGATTCCTGGAGAACTTGGTCAGCTCAGCAGGCTTAAAACATTTAGCGTGGCCAACAATCAACTGTCTGGGCAAGTCCCCAACATCAAGAATGGCACAATCGGAGCGGACAGCTATGCAAACAATCCTGGACTTTGTGGTGCTCCTTTGGAAAGTTGCCAGGCAGCTGCAAAGAAGTCTAACAGTGTAGTTATAGTGGCAGCAGGTGTCAGGGGTGCAACTTTTGCAGCATTAGTTGTGATTATTGGTTTGTTCTTCTTCATGCGTAGAGTGTCTGCAAGGAAGAAGGAAGAGGACCCTGAAGGCAACAAATGGGCAAGGAGTTTGAAGGGAACTAAAGGAATCAAGGTTTCCATGTTCAAGAAGTCGGTTTCTAAAATGAGACTGAGTGATCTCATGAAGGCTACCAACAATTTCAGCAAAGAAAATATCATTGGCTCAGGAAGAACTGGAACTGTTTACAAGGCATTGCTTGATGATGGCACCTCACTTATGGTCAAGAGGCTGCAGGAATCTCAACACTCTGAGAAAGAGTTTCTGTCTGAGATGTCTACTCTGGGTGGTGTAGAACACCGTAACTTGGTTCCCCTTTTGGGTTTTTGTGTGGCAAAGAAGGAAAGGCTTTTGGTGTATAAGCACATGCCTCATGGTACCCTCCATGAGCAGCTACATCCTGTGGAAGCTGATGGTGCCAAGATTATGGACTGGCCTACAAGGCTGAAAATTGGGATAGGAGCAGCCAGAGGATTAGCGTGGCTTCATCATAACTGTAATCCTCGAATTATCCACCGAAACATAAGCTCCAAATGCATCTTACTAGATGCAGATTTTGAGCCGAAGATTTCTGATTTTGGCCTAGCTAGGCTCATGAATCCAATTGATACACATTTGAGTACTTTTGTGAATGGGGAGTTCGGAGATCTGGGTTATGTGGCTCCTGAGTATACAAGAACTTTAGTGGCCACTCCCAAGGGGGATGTTTACAGCTTTGGAACTGTTCTTCTTGAGTTGGTTACTGGTGAAAGAGCTACCCATATTTCTAAAGCTCCAGAAGATTTCAAAGGAAACTTGGTGGAATGGATCTTGCAGCTGTCAAGCAAATCTCAACTCACAGATGCGATTGATAAATCCTTGATTGGAAAGGGTGTGGATGAGGAGGTTTTTCAGTTCCTTAAAGTTGCATGCAACTGTGTGGTGCCTACTTCCAAGGAGAGGCCTACTATGTTTGAAGTATTCCAGCTTCTACGAGCCATCGGACAGAAATATAACTTCACAATTGAAGAGGAGATGTCGACGCCTTCAGACACTGCTGATTGTGCAGGAGAACTCATTGTTGCTCGAGAGATGATGGAGATTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0031348 GO:0033612 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0060548 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

610

Amino Acids

66.93

Weight (kDa)

7.19

Isoelectric Point (pI)

29.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	122 - 179	1.6e-06	Leucine rich repeat
Pkinase	PF00069	296 - 570	5.7e-34	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	299 - 567	4.5e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004418)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48380
fragaria_vesca	FvH4_2g17440 FvH4_2g17440 FvH4_2g17440
malus_domestica	MD05G1153300.v1.1
prunus_persica	Prupe.8G186700_v2.0.a1
pyrus_communis	pycom05g14770 pycom10g12770
rosa_chinensis	RchiOBHm_Chr6g0248541 RchiOBHm_Chr6g0282151
rosa_laevigata	RLG00000012905 RLG00000015144
rosa_multiflora	Rmu_sc0002553.1_g000025 Rmu_sc0002553.1_g000026 Rmu_sc0011940.1_g000001 Rmu_sc0021471.1_g000006
rosa_roxburghii	Rroxscaffold_7G00186440 Rroxscaffold_7G00213230
rosa_rugosa	Rorug05G0532000 Rorug06G0145800
rosa_samantha	Rh6AG048900 Rh6AG258800 Rh6BG044100 Rh6BG044400 Rh6BG262100 Rh6CG041000 Rh6CG261100 Rh6DG037200 Rh6DG253100
rosa_wichuraiana	Rw6G004150 Rw6G004230 Rw6G022400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	665
Acc36I	ACCTGC	1 cut(s)	665
Acc65I	GGTACC	1 cut(s)	1121
AccB1I	GGYRCC	5 cut(s)	195, 953, 1121, 1160, 1666
AccBSI	CCGCTC	1 cut(s)	578
AccI	GTMKAC	3 cut(s)	1022, 1415, 1763
AciI	CCGC	2 cut(s)	233, 578
AclWI	GGATC	1 cut(s)	1559
AcoI	YGGCCR	2 cut(s)	522, 1431
AcuI	CTGAAG	2 cut(s)	792, 1755
AcyI	GRCGYC	1 cut(s)	1766
AfaI	GTAC	4 cut(s)	280, 427, 1123, 1366
AfiI	CCNNNNNNNGG	4 cut(s)	135, 1051, 1064, 1683
AflIII	ACRYGT	1 cut(s)	120
AgsI	TTSAA	6 cut(s)	306, 799, 832, 1531, 1700, 1751
AjnI	CCWGG	4 cut(s)	407, 478, 598, 630
AjuI	GAANNNNNNNTTGG	4 cut(s)	175, 207, 888, 920
Alw21I	GWGCWC	3 cut(s)	27, 378, 616
Alw26I	GTCTC	1 cut(s)	847
AlwI	GGATC	1 cut(s)	1559
AlwNI	CAGNNNCTG	1 cut(s)	1778
Ama87I	CYCGRG	1 cut(s)	1809
AoxI	GGCC	6 cut(s)	250, 522, 1178, 1324, 1431, 1685
ApeKI	GCWGC	8 cut(s)	635, 638, 671, 698, 976, 1135, 1208, 1558
AseI	ATTAAT	1 cut(s)	1827
Asp718I	GGTACC	1 cut(s)	1121
AspS9I	GGNCC	2 cut(s)	109, 766
AsuHPI	GGTGA	1 cut(s)	1502
AvaI	CYCGRG	1 cut(s)	1809
AvaII	GGWCC	2 cut(s)	109, 766
BalI	TGGCCA	2 cut(s)	524, 1433
BanI	GGYRCC	5 cut(s)	195, 953, 1121, 1160, 1666
BanII	GRGCYC	1 cut(s)	378
BarI	GAAGNNNNNNTAC	2 cut(s)	1695, 1727
BauI	CACGAG	1 cut(s)	261
Bbv12I	GWGCWC	3 cut(s)	27, 378, 616
BbvI	GCAGC	8 cut(s)	625, 647, 683, 710, 963, 1147, 1220, 1570
BccI	CCATC	4 cut(s)	944, 1151, 1730, 1813
BciT130I	CCWGG	4 cut(s)	409, 480, 600, 632
BcoDI	GTCTC	1 cut(s)	847
BfaI	CTAG	3 cut(s)	1289, 1328, 1332
BfmI	CTRYAG	1 cut(s)	977
BfuAI	ACCTGC	1 cut(s)	665
BglI	GCCNNNNNGGC	1 cut(s)	1186
BglII	AGATCT	2 cut(s)	298, 1390
BisI	GCNGC	8 cut(s)	636, 639, 672, 699, 977, 1136, 1209, 1559
BlpI	GCTNAGC	1 cut(s)	497
BlsI	GCNGC	8 cut(s)	637, 640, 673, 700, 978, 1137, 1210, 1560
BmcAI	AGTACT	1 cut(s)	1366
Bme1390I	CCNGG	4 cut(s)	409, 480, 600, 632
Bme18I	GGWCC	2 cut(s)	109, 766
BmeT110I	CYCGRG	1 cut(s)	1809
BmgT120I	GGNCC	2 cut(s)	109, 766
BmiI	GGNNCC	9 cut(s)	111, 197, 768, 955, 1056, 1123, 1162, 1407, 1668
BmrFI	CCNGG	4 cut(s)	409, 480, 600, 632
BmsI	GCATC	3 cut(s)	1282, 1290, 1576
BpmI	CTGGAG	2 cut(s)	501, 1503
Bpu10I	CCTNAGC	1 cut(s)	910
Bpu1102I	GCTNAGC	1 cut(s)	497
BpuEI	CTTGAG	3 cut(s)	103, 392, 1496
BsaHI	GRCGYC	1 cut(s)	1766
BsaJI	CCNNGG	4 cut(s)	55, 407, 1440, 1677
BsaXI	ACNNNNNCTCC	2 cut(s)	1256, 1286
Bsc4I	CCNNNNNNNGG	4 cut(s)	135, 1051, 1064, 1683
Bse1I	ACTGG	3 cut(s)	925, 1181, 1492
Bse3DI	GCAATG	1 cut(s)	938
BseBI	CCWGG	4 cut(s)	409, 480, 600, 632
BseDI	CCNNGG	4 cut(s)	55, 407, 1440, 1677
BseGI	GGATG	3 cut(s)	1141, 1453, 1627
BseLI	CCNNNNNNNGG	4 cut(s)	135, 1051, 1064, 1683
BseMI	GCAATG	1 cut(s)	938
BseMII	CTCAG	8 cut(s)	331, 408, 511, 848, 924, 987, 1005, 1401
BseNI	ACTGG	3 cut(s)	925, 1181, 1492
BseRI	GAGGAG	2 cut(s)	1640, 1769
BseXI	GCAGC	8 cut(s)	625, 647, 683, 710, 963, 1147, 1220, 1570
BsgI	GTGCAG	1 cut(s)	1809
BshFI	GGCC	6 cut(s)	252, 524, 1180, 1326, 1433, 1687
BshNI	GGYRCC	5 cut(s)	195, 953, 1121, 1160, 1666
BsiHKAI	GWGCWC	3 cut(s)	27, 378, 616
BsiHKCI	CYCGRG	1 cut(s)	1809
BslFI	GGGAC	2 cut(s)	255, 533
BslI	CCNNNNNNNGG	4 cut(s)	135, 1051, 1064, 1683
BsmAI	GTCTC	1 cut(s)	847
BsmFI	GGGAC	2 cut(s)	255, 533
BsnI	GGCC	6 cut(s)	252, 524, 1180, 1326, 1433, 1687
BsoBI	CYCGRG	1 cut(s)	1809
Bsp1286I	GDGCHC	3 cut(s)	27, 378, 616
Bsp1407I	TGTACA	1 cut(s)	278
Bsp143I	GATC	4 cut(s)	298, 862, 1390, 1551
Bsp1720I	GCTNAGC	1 cut(s)	497
Bsp19I	CCATGG	1 cut(s)	55
BspACI	CCGC	2 cut(s)	233, 578
BspANI	GGCC	6 cut(s)	252, 524, 1180, 1326, 1433, 1687
BspCNI	CTCAG	8 cut(s)	330, 407, 510, 849, 923, 988, 1006, 1402
BspHI	TCATGA	2 cut(s)	867, 1338
BspLI	GGNNCC	9 cut(s)	111, 197, 768, 955, 1056, 1123, 1162, 1407, 1668
BspMAI	CTGCAG	1 cut(s)	981
BspMI	ACCTGC	1 cut(s)	665
BspPI	GGATC	1 cut(s)	1559
BspT107I	GGYRCC	5 cut(s)	195, 953, 1121, 1160, 1666
BsrBI	CCGCTC	1 cut(s)	578
BsrDI	GCAATG	1 cut(s)	938
BsrGI	TGTACA	1 cut(s)	278
BsrI	ACTGG	3 cut(s)	925, 1181, 1492
BssECI	CCNNGG	4 cut(s)	55, 407, 1440, 1677
BssMI	GATC	4 cut(s)	298, 862, 1390, 1551
BssNAI	GTATAC	1 cut(s)	1416
BssNI	GRCGYC	1 cut(s)	1766
BssSI	CACGAG	1 cut(s)	261
BssT1I	CCWWGG	3 cut(s)	55, 1440, 1677
Bst1107I	GTATAC	1 cut(s)	1416
Bst2BI	CACGAG	1 cut(s)	261
Bst2UI	CCWGG	4 cut(s)	409, 480, 600, 632
Bst4CI	ACNGT	7 cut(s)	537, 656, 928, 1046, 1241, 1468, 1661
Bst6I	CTCTTC	3 cut(s)	136, 756, 1746
BstACI	GRCGYC	1 cut(s)	1766
BstAPI	GCANNNNNTGC	1 cut(s)	695
BstAUI	TGTACA	1 cut(s)	278
BstC8I	GCNNGC	3 cut(s)	231, 503, 1654
BstDSI	CCRYGG	1 cut(s)	55
BstF5I	GGATG	3 cut(s)	1141, 1453, 1627
BstKTI	GATC	4 cut(s)	301, 865, 1393, 1554
BstMAI	GTCTC	1 cut(s)	847
BstMBI	GATC	4 cut(s)	298, 862, 1390, 1551
BstMWI	GCNNNNNNNGC	7 cut(s)	291, 502, 695, 1160, 1186, 1278, 1787
BstNI	CCWGG	4 cut(s)	409, 480, 600, 632
BstNSI	RCATGY	3 cut(s)	124, 1114, 1656
BstSCI	CCNGG	4 cut(s)	407, 478, 598, 630
BstSFI	CTRYAG	1 cut(s)	977
BstV1I	GCAGC	8 cut(s)	625, 647, 683, 710, 963, 1147, 1220, 1570
BstX2I	RGATCY	3 cut(s)	298, 1390, 1551
BstXI	CCANNNNNNTGG	1 cut(s)	1171
BstYI	RGATCY	3 cut(s)	298, 1390, 1551
BstZ17I	GTATAC	1 cut(s)	1416
BsuRI	GGCC	6 cut(s)	252, 524, 1180, 1326, 1433, 1687
BtgI	CCRYGG	1 cut(s)	55
BtsCI	GGATG	3 cut(s)	1141, 1453, 1627
BtsI	GCAGTG	1 cut(s)	1776
BtsIMutI	CAGTG	3 cut(s)	325, 661, 1776
BveI	ACCTGC	1 cut(s)	665
Cac8I	GCNNGC	3 cut(s)	231, 503, 1654
CaiI	CAGNNNCTG	1 cut(s)	1778
CciI	TCATGA	2 cut(s)	867, 1338
Cfr13I	GGNCC	2 cut(s)	109, 766
CseI	GACGC	1 cut(s)	1774
Csp6I	GTAC	4 cut(s)	279, 426, 1122, 1365
CspCI	CAANNNNNGTGG	2 cut(s)	301, 336
CviQI	GTAC	4 cut(s)	279, 426, 1122, 1365
DpnI	GATC	4 cut(s)	300, 864, 1392, 1553
DpnII	GATC	4 cut(s)	298, 862, 1390, 1551
DraI	TTTAAA	1 cut(s)	219
EaeI	YGGCCR	2 cut(s)	522, 1431
Eam1104I	CTCTTC	3 cut(s)	136, 756, 1746
EarI	CTCTTC	3 cut(s)	136, 756, 1746
Ecl136II	GAGCTC	1 cut(s)	376
Eco130I	CCWWGG	3 cut(s)	55, 1440, 1677
Eco147I	AGGCCT	1 cut(s)	1687
Eco24I	GRGCYC	1 cut(s)	378
Eco47I	GGWCC	2 cut(s)	109, 766
Eco53kI	GAGCTC	1 cut(s)	376
Eco57I	CTGAAG	2 cut(s)	792, 1755
Eco88I	CYCGRG	1 cut(s)	1809
EcoICRI	GAGCTC	1 cut(s)	376
EcoO109I	RGGNCCY	2 cut(s)	109, 766
EcoRII	CCWGG	4 cut(s)	407, 478, 598, 630
EcoT14I	CCWWGG	3 cut(s)	55, 1440, 1677
EcoT22I	ATGCAT	1 cut(s)	1283
EcoT38I	GRGCYC	1 cut(s)	378
ErhI	CCWWGG	3 cut(s)	55, 1440, 1677
FaqI	GGGAC	2 cut(s)	255, 533
FblI	GTMKAC	3 cut(s)	1022, 1415, 1763
Fnu4HI	GCNGC	8 cut(s)	636, 639, 672, 699, 977, 1136, 1209, 1559
FokI	GGATG	3 cut(s)	1128, 1460, 1634
FriOI	GRGCYC	1 cut(s)	378
Fsp4HI	GCNGC	8 cut(s)	636, 639, 672, 699, 977, 1136, 1209, 1559
FspBI	CTAG	3 cut(s)	1289, 1328, 1332
GluI	GCNGC	8 cut(s)	636, 639, 672, 699, 977, 1136, 1209, 1559
GsuI	CTGGAG	2 cut(s)	501, 1503
HaeIII	GGCC	6 cut(s)	252, 524, 1180, 1326, 1433, 1687
HgaI	GACGC	1 cut(s)	1774
Hin1I	GRCGYC	1 cut(s)	1766
HincII	GTYRAC	3 cut(s)	126, 465, 1764
HindII	GTYRAC	3 cut(s)	126, 465, 1764
HindIII	AAGCTT	2 cut(s)	227, 283
HinfI	GANTC	7 cut(s)	162, 183, 243, 475, 813, 983, 1342
HpaI	GTTAAC	2 cut(s)	126, 465
HphI	GGTGA	1 cut(s)	1502
Hpy166II	GTNNAC	7 cut(s)	126, 465, 931, 1023, 1416, 1453, 1764
Hpy188I	TCNGA	9 cut(s)	435, 474, 575, 997, 1015, 1318, 1388, 1727, 1774
Hpy8I	GTNNAC	7 cut(s)	126, 465, 931, 1023, 1416, 1453, 1764
Hpy99I	CGWCG	2 cut(s)	370, 1768
HpyAV	CCTTC	7 cut(s)	152, 751, 767, 793, 865, 1078, 1779
HpyCH4III	ACNGT	7 cut(s)	537, 656, 928, 1046, 1241, 1468, 1661
HpyCH4IV	ACGT	1 cut(s)	365
HpyF10VI	GCNNNNNNNGC	7 cut(s)	291, 502, 695, 1160, 1186, 1278, 1787
HpySE526I	ACGT	1 cut(s)	365
Hsp92I	GRCGYC	1 cut(s)	1766
KpnI	GGTACC	1 cut(s)	1125
KspAI	GTTAAC	2 cut(s)	126, 465
Kzo9I	GATC	4 cut(s)	298, 862, 1390, 1551
LmnI	GCTCC	7 cut(s)	323, 575, 619, 1205, 1277, 1411, 1522
Lsp1109I	GCAGC	8 cut(s)	625, 647, 683, 710, 963, 1147, 1220, 1570
LweI	GCATC	3 cut(s)	1282, 1290, 1576
MaeI	CTAG	3 cut(s)	1289, 1328, 1332
MaeII	ACGT	1 cut(s)	365
MaeIII	GTNAC	3 cut(s)	170, 1046, 1483
MalI	GATC	4 cut(s)	300, 864, 1392, 1553
MbiI	CCGCTC	1 cut(s)	578
MboI	GATC	4 cut(s)	298, 862, 1390, 1551
MfeI	CAATTG	3 cut(s)	421, 1347, 1746
MflI	RGATCY	3 cut(s)	298, 1390, 1551
MhlI	GDGCHC	3 cut(s)	27, 378, 616
MlsI	TGGCCA	2 cut(s)	524, 1433
MluCI	AATT	9 cut(s)	274, 402, 421, 883, 1194, 1251, 1347, 1746, 1828
MluNI	TGGCCA	2 cut(s)	524, 1433
MlyI	GAGTC	1 cut(s)	237
MmeI	TCCRAC	1 cut(s)	408
Mox20I	TGGCCA	2 cut(s)	524, 1433
Mph1103I	ATGCAT	1 cut(s)	1283
MscI	TGGCCA	2 cut(s)	524, 1433
MslI	CAYNNNNRTG	2 cut(s)	20, 201
Msp20I	TGGCCA	2 cut(s)	524, 1433
MspA1I	CMGCKG	2 cut(s)	638, 1561
MspR9I	CCNGG	4 cut(s)	409, 480, 600, 632
MunI	CAATTG	3 cut(s)	421, 1347, 1746
MvaI	CCWGG	4 cut(s)	409, 480, 600, 632
MwoI	GCNNNNNNNGC	7 cut(s)	291, 502, 695, 1160, 1186, 1278, 1787
NcoI	CCATGG	1 cut(s)	55
NdeII	GATC	4 cut(s)	298, 862, 1390, 1551
NlaIV	GGNNCC	9 cut(s)	111, 197, 768, 955, 1056, 1123, 1162, 1407, 1668
NsiI	ATGCAT	1 cut(s)	1283
NspI	RCATGY	3 cut(s)	124, 1114, 1656
PaeI	GCATGC	1 cut(s)	1656
PaeR7I	CTCGAG	1 cut(s)	1809
PagI	TCATGA	2 cut(s)	867, 1338
PaqCI	CACCTGC	1 cut(s)	665
PceI	AGGCCT	1 cut(s)	1687
PciI	ACATGT	1 cut(s)	120
PfeI	GAWTC	6 cut(s)	162, 183, 475, 813, 983, 1342
PfoI	TCCNGGA	2 cut(s)	478, 598
PkrI	GCNGC	8 cut(s)	637, 640, 673, 700, 978, 1137, 1210, 1560
PleI	GAGTC	1 cut(s)	237
PpsI	GAGTC	1 cut(s)	237
PpuMI	RGGWCCY	2 cut(s)	109, 766
PscI	ACATGT	1 cut(s)	120
PshBI	ATTAAT	1 cut(s)	1827
Psp124BI	GAGCTC	1 cut(s)	378
Psp5II	RGGWCCY	2 cut(s)	109, 766
Psp6I	CCWGG	4 cut(s)	407, 478, 598, 630
PspGI	CCWGG	4 cut(s)	407, 478, 598, 630
PspN4I	GGNNCC	9 cut(s)	111, 197, 768, 955, 1056, 1123, 1162, 1407, 1668
PspPI	GGNCC	2 cut(s)	109, 766
PspPPI	RGGWCCY	2 cut(s)	109, 766
PstI	CTGCAG	1 cut(s)	981
PstNI	CAGNNNCTG	1 cut(s)	1778
PsuI	RGATCY	3 cut(s)	298, 1390, 1551
PvuII	CAGCTG	2 cut(s)	638, 1561
RsaI	GTAC	4 cut(s)	280, 427, 1123, 1366
RsaNI	GTAC	4 cut(s)	279, 426, 1122, 1365
RseI	CAYNNNNRTG	2 cut(s)	20, 201
SacI	GAGCTC	1 cut(s)	378
SalI	GTCGAC	1 cut(s)	1762
SatI	GCNGC	8 cut(s)	636, 639, 672, 699, 977, 1136, 1209, 1559
Sau3AI	GATC	4 cut(s)	298, 862, 1390, 1551
Sau96I	GGNCC	2 cut(s)	109, 766
ScaI	AGTACT	1 cut(s)	1366
SchI	GAGTC	1 cut(s)	237
ScrFI	CCNGG	4 cut(s)	409, 480, 600, 632
SduI	GDGCHC	3 cut(s)	27, 378, 616
SfaNI	GCATC	3 cut(s)	1282, 1290, 1576
SfcI	CTRYAG	1 cut(s)	977
Sfr274I	CTCGAG	1 cut(s)	1809
SinI	GGWCC	2 cut(s)	109, 766
SlaI	CTCGAG	1 cut(s)	1809
SmiMI	CAYNNNNRTG	2 cut(s)	20, 201
SmlI	CTYRAG	4 cut(s)	82, 371, 1475, 1809
SmoI	CTYRAG	4 cut(s)	82, 371, 1475, 1809
SphI	GCATGC	1 cut(s)	1656
Sse9I	AATT	9 cut(s)	274, 402, 421, 883, 1194, 1251, 1347, 1746, 1828
SseBI	AGGCCT	1 cut(s)	1687
SsiI	CCGC	2 cut(s)	233, 578
SspMI	CTAG	3 cut(s)	1289, 1328, 1332
SstI	GAGCTC	1 cut(s)	378
StuI	AGGCCT	1 cut(s)	1687
StyD4I	CCNGG	4 cut(s)	407, 478, 598, 630
StyI	CCWWGG	3 cut(s)	55, 1440, 1677
TaaI	ACNGT	7 cut(s)	537, 656, 928, 1046, 1241, 1468, 1661
TaiI	ACGT	1 cut(s)	368
TaqI	TCGA	4 cut(s)	186, 1249, 1763, 1810
TasI	AATT	9 cut(s)	274, 402, 421, 883, 1194, 1251, 1347, 1746, 1828
TatI	WGTACW	3 cut(s)	278, 425, 1364
TfiI	GAWTC	6 cut(s)	162, 183, 475, 813, 983, 1342
TscAI	CASTG	3 cut(s)	325, 661, 1783
TseI	GCWGC	8 cut(s)	635, 638, 671, 698, 976, 1135, 1208, 1558
TspDTI	ATGAA	7 cut(s)	123, 154, 349, 721, 884, 1220, 1355
TspRI	CASTG	3 cut(s)	325, 661, 1783
VpaK11BI	GGWCC	2 cut(s)	109, 766
VspI	ATTAAT	1 cut(s)	1827
XceI	RCATGY	3 cut(s)	124, 1114, 1656
XcmI	CCANNNNNNNNNTGG	1 cut(s)	260
XhoI	CTCGAG	1 cut(s)	1809
XmiI	GTMKAC	3 cut(s)	1022, 1415, 1763
XspI	CTAG	3 cut(s)	1289, 1328, 1332
ZrmI	AGTACT	1 cut(s)	1366
Zsp2I	ATGCAT	1 cut(s)	1283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.