Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0010289

Physical Location & Seq

Genomic Coordinates Forward (+)

5616233 .. 5619569

Gene Structure 3337 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG054800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGAAGAACCAAGTTCTCTTGCATCTTTCACTTTCAGTATTTCTCCTAATAGTCCCCATTGTAGCTCAACAATTTGCATGTGACACCAAAGCTTCCACAACTAGCCAATTTCCCTTCTGCAACACTTCTCTGCCTTACGAGGACCGAGCCAAGGACTTGGTCTCGCGCCTCACTCTTCAAGAAAAGGTCCAACAACTAGTGGATGCATCCACAGGCATATCTCGGCTAGGCGTGCCTAAATATGAGTGGTGGTCCGAGGCACTCCATGGTGTTTCGAATGTTGGCCCAGGCACTCGCTTTAATGCCACGGTGCCTGGTGCCACTAGTTTCCCAGCAGTGATTCTATCCGCAGCGAGTTTTAATGAATCACTGTGGTTGAGGATGGGGCAAGTTGTGTCAACCGAGGCTCGAGCCATGTACAATTTGGGTCTAGCCGGGTTGACATTTTGGAGTCCTAACGTCAATGTGTTCCGTGACCCGAGATGGGGTCGAGCGCAAGAGACACCGGGCGAGGACCCCTTAGTGGTGTCAAATTATGCAGTGAACTATGTCAAGGGTCTCCAAGACGTTGGTGAGGGCAATGCCACTGGTGACAGGCTCAAGGTCTCAAGCTGCTGCAAGCATTACACTGCTTATGATGTTGATAATTGGAAAGGTGTTGATAGATTTCACTTTGATGCTCAGGTGACAAAGCAGGACATGGAGGATACATACCAGCCACCATTCAAAAGTTGTGTGCAGGAGGGCCATGTTAGCAGTGTCATGTGTTCTTATAATAGGGTGAATGGGATTCCCACCTGTGCTGACCCAGACCTGCTGGAAGGGGTAGTCAGAGGCCAATGGGGTCTAGATGGATATATAGTTTCTGACTGTGATTCTGTGGAAGTTTATTACGATGCAATCCATTATACTGAAACACGTGAGGATGCAGCAGCCCTGGCCTTGAAAGCAGGTCTAAACATGAACTGTGGGGATTATCTAGGGAAGTACACAGAAAATGCAGTCAACTTGAAGAAAGTGGAAGAATCTGTTGTTGATCAGTCCTTGATATACAACTACATAGTCCTCATGAGGCTTGGCTTCTTTGATGGTGATCCGAAACTGCTCGAATTTGGAAACCTTGGGGTATCGGATGTTTGCAGTGACGAGCATAAAGCACTGGCACTTGACGCTGCTAAGCAGGGCATAGTTTTGCTAGATAACAATGGAGCTCTTCCTTTGTCCCCAACTACAACCAAGAATTTGGCCATTATTGGACCTAATGCAAATGCCACTAACGTTATGATAAGCAACTATGCTGGAATACCATGTAGCTACACTAGCCCTCTACAAGGGTTACAGAAGTATGTGTCGACCGCAAAATATGAGCCCGGGTGTAGCAGTGTAAAATGTGCTGATGAGAGCCTCATCGGGGCAGCGGTTCAGGCTGCTGCCACAGCTAATGCAGTGGTGTTAGTAGTGGGGCTTGATCAGTCCATCGAAGCAGAAGGGCTAGACAGAGTGAACTTGACTTTACCAGGCTTTCAGGAAAAGCTTGTGGAGCAAGTTGTTGATGCAACAAATGGAACAGTCATTCTGGTCATTATGTCAGCTGGTCCAATTGATGTTTCGTTTGCTAAAAATTTGAGCAAAGTAGGAGGGATTCTATGGGTTGGGTATCCAGGTCAAGATGGAGGAGATGCCATAGCTCAAGTCATATTTGGGGACTATAATCCAGCTGGGAAGAGTCCTTTTACATGGTACCCACAGGAGTATGTAGACCAACTGCCAATGACAGACATGAACATGCGGGCCAACACTAGTAGAAACTACCCTGGAAGAACATATAGGTTCTACACTGGAAAAACCGTCTATAAGTTTGGTCACGGGCTCAGCTATTCAACCTTTTCCAAGTTCATAATCTCTGCACCTTCCACTCTACTCATCCAATCAAACTCCAATGCTACTTTCAACCCACACTATAACATTCAATTGGAACCCATTTCCGTCACCCAAGAAATTGACATATCAAGCATAGATTGCCAGAGCTTAGCATTTGATCTAGTTGTTGGGGTCAGGAACAAAGGCCCAATAGATGGCTCCCACGTGGTGCTCGCTTTCTGGAAACCACCACTCTCACAAGGGTTGATTGGGGCACCAAAGTTGCAGCTTGTGGGTTTTGAAAGAGTGAAGGTGAAGAATAGAAAGACAAAGTCATTGAGAATGAGGGTGGATGTGTGCAAGGGACTGAACTTTGTGGATAGTGAAGGGAAGAGGAAGTTGGTCACCGGGCTGCACACCATTCTCGTCGGCTCTCCTAGTGAGAAACAAGTGAAGCACTACCTCAATGTTAGGCTGGCTGCAGAGAAACATGAACTGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

787

Amino Acids

85.89

Weight (kDa)

5.88

Isoelectric Point (pI)

31.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	106 - 348	3.4e-34	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	396 - 626	2e-50	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	695 - 765	5.6e-09	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010289)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19620
fragaria_vesca	FvH4_2g04280
malus_domestica	MD05G1099400.v1.1
prunus_persica	Prupe.8G143900_v2.0.a1
pyrus_communis	pycom05g09830
rosa_chinensis	RchiOBHm_Chr6g0253671 RchiOBHm_Chr6g0253691
rosa_laevigata	RLG00000014993
rosa_multiflora	Rmu_sc0000331.1_g000019 Rmu_sc0001351.1_g000008 Rmu_sc0006378.1_g000004 Rmu_sc0017598.1_g000001
rosa_roxburghii	Rroxscaffold_7G00211710
rosa_rugosa	Rorug05G0545400 Rorug05G0545500 Rorug05G0545600 Rorug05G0545700
rosa_samantha	Rh6AG061200 Rh6CG054800 Rh6DG052300
rosa_wichuraiana	Rw6G005440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	774
Acc36I	ACCTGC	2 cut(s)	822, 941
Acc65I	GGTACC	1 cut(s)	1740
AccB1I	GGYRCC	4 cut(s)	310, 317, 1740, 2134
AccB7I	CCANNNNNTGG	1 cut(s)	2075
AccI	GTMKAC	2 cut(s)	1352, 1758
AccII	CGCG	1 cut(s)	166
AciI	CCGC	4 cut(s)	348, 1356, 1418, 1789
AclI	AACGTT	1 cut(s)	1278
AclWI	GGATC	1 cut(s)	1088
AcoI	YGGCCR	1 cut(s)	1245
AcsI	RAATTY	3 cut(s)	1109, 1240, 1621
AcvI	CACGTG	2 cut(s)	920, 2086
AdeI	CACNNNGTG	1 cut(s)	2089
AfaI	GTAC	3 cut(s)	419, 989, 1742
AfiI	CCNNNNNNNGG	7 cut(s)	151, 484, 485, 523, 1331, 1411, 2075
AflIII	ACRYGT	1 cut(s)	917
AgsI	TTSAA	8 cut(s)	179, 727, 946, 1012, 1881, 1951, 1970, 2162
AhlI	ACTAGT	3 cut(s)	196, 323, 1799
AjnI	CCWGG	6 cut(s)	286, 313, 936, 1516, 1660, 1813
AjuI	GAANNNNNNNTTGG	6 cut(s)	1591, 1623, 1931, 1963, 2243, 2275
Alw21I	GWGCWC	2 cut(s)	1213, 2094
Alw26I	GTCTC	4 cut(s)	166, 494, 563, 610
AlwI	GGATC	1 cut(s)	1088
Ama87I	CYCGRG	3 cut(s)	408, 478, 1370
AoxI	GGCC	7 cut(s)	283, 745, 835, 939, 1245, 1791, 2065
ApoI	RAATTY	3 cut(s)	1109, 1240, 1621
Asp718I	GGTACC	1 cut(s)	1740
AspLEI	GCGC	2 cut(s)	168, 496
AsuC2I	CCSGG	5 cut(s)	436, 507, 1371, 1372, 2269
AsuHPI	GGTGA	8 cut(s)	584, 602, 697, 793, 1103, 1981, 2185, 2257
AsuII	TTCGAA	1 cut(s)	275
AvaI	CYCGRG	3 cut(s)	408, 478, 1370
AvaII	GGWCC	6 cut(s)	142, 187, 252, 514, 1256, 1595
BaeGI	GKGCMC	1 cut(s)	2137
BalI	TGGCCA	1 cut(s)	1247
BanI	GGYRCC	4 cut(s)	310, 317, 1740, 2134
BanII	GRGCYC	3 cut(s)	1213, 1371, 1872
BbrPI	CACGTG	2 cut(s)	920, 2086
Bbv12I	GWGCWC	2 cut(s)	1213, 2094
BccI	CCATC	7 cut(s)	376, 477, 845, 1082, 1484, 1664, 2069
BciT130I	CCWGG	6 cut(s)	288, 315, 938, 1518, 1662, 1815
BciVI	GTATCC	2 cut(s)	700, 1668
BclI	TGATCA	2 cut(s)	1036, 1468
BcnI	CCSGG	5 cut(s)	436, 507, 1371, 1372, 2269
BcoDI	GTCTC	4 cut(s)	166, 494, 563, 610
BcuI	ACTAGT	3 cut(s)	196, 323, 1799
BfmI	CTRYAG	1 cut(s)	2340
BfuAI	ACCTGC	2 cut(s)	822, 941
BfuI	GTATCC	2 cut(s)	700, 1668
BlpI	GCTNAGC	3 cut(s)	1176, 1871, 2029
Bme18I	GGWCC	6 cut(s)	142, 187, 252, 514, 1256, 1595
BmeT110I	CYCGRG	3 cut(s)	408, 478, 1370
BmiI	GGNNCC	7 cut(s)	312, 319, 516, 1742, 1977, 2080, 2136
BmsI	GCATC	8 cut(s)	31, 193, 215, 667, 886, 916, 1543, 1669
Bpu10I	CCTNAGC	1 cut(s)	681
Bpu1102I	GCTNAGC	3 cut(s)	1176, 1871, 2029
Bpu14I	TTCGAA	1 cut(s)	275
BpuEI	CTTGAG	3 cut(s)	584, 592, 1674
BpuMI	CCSGG	5 cut(s)	436, 507, 1371, 1372, 2269
BsaAI	YACGTR	2 cut(s)	920, 2086
BsaBI	GATNNNNATC	1 cut(s)	1092
BsaI	GGTCTC	3 cut(s)	166, 563, 610
Bsc4I	CCNNNNNNNGG	7 cut(s)	151, 484, 485, 523, 1331, 1411, 2075
Bse1I	ACTGG	3 cut(s)	592, 1164, 1843
Bse3DI	GCAATG	1 cut(s)	586
Bse8I	GATNNNNATC	1 cut(s)	1092
BseBI	CCWGG	6 cut(s)	288, 315, 938, 1518, 1662, 1815
BseGI	GGATG	7 cut(s)	206, 208, 387, 931, 1138, 1923, 2218
BseJI	GATNNNNATC	1 cut(s)	1092
BseLI	CCNNNNNNNGG	7 cut(s)	151, 484, 485, 523, 1331, 1411, 2075
BseMI	GCAATG	1 cut(s)	586
BseMII	CTCAG	2 cut(s)	695, 1885
BseNI	ACTGG	3 cut(s)	592, 1164, 1843
BseRI	GAGGAG	1 cut(s)	1689
BseSI	GKGCMC	1 cut(s)	2137
BseYI	CCCAGC	2 cut(s)	331, 1718
BsgI	GTGCAG	3 cut(s)	758, 1890, 2258
Bsh1236I	CGCG	1 cut(s)	166
Bsh1285I	CGRYCG	1 cut(s)	1356
BshFI	GGCC	7 cut(s)	285, 747, 837, 941, 1247, 1793, 2067
BshNI	GGYRCC	4 cut(s)	310, 317, 1740, 2134
BsiEI	CGRYCG	1 cut(s)	1356
BsiHKAI	GWGCWC	2 cut(s)	1213, 2094
BsiHKCI	CYCGRG	3 cut(s)	408, 478, 1370
BsiSI	CCGG	4 cut(s)	435, 506, 1371, 2268
BslFI	GGGAC	4 cut(s)	38, 1207, 1718, 2238
BslI	CCNNNNNNNGG	7 cut(s)	151, 484, 485, 523, 1331, 1411, 2075
BsmAI	GTCTC	4 cut(s)	166, 494, 563, 610
BsmFI	GGGAC	4 cut(s)	38, 1207, 1718, 2238
BsnI	GGCC	7 cut(s)	285, 747, 837, 941, 1247, 1793, 2067
Bso31I	GGTCTC	3 cut(s)	166, 563, 610
BsoBI	CYCGRG	3 cut(s)	408, 478, 1370
Bsp119I	TTCGAA	1 cut(s)	275
Bsp1286I	GDGCHC	5 cut(s)	1213, 1371, 1872, 2094, 2137
Bsp1407I	TGTACA	1 cut(s)	417
Bsp143I	GATC	4 cut(s)	1036, 1093, 1468, 2038
Bsp1720I	GCTNAGC	3 cut(s)	1176, 1871, 2029
Bsp19I	CCATGG	1 cut(s)	265
BspACI	CCGC	4 cut(s)	348, 1356, 1418, 1789
BspANI	GGCC	7 cut(s)	285, 747, 837, 941, 1247, 1793, 2067
BspCNI	CTCAG	2 cut(s)	694, 1884
BspFNI	CGCG	1 cut(s)	166
BspHI	TCATGA	1 cut(s)	1068
BspLI	GGNNCC	7 cut(s)	312, 319, 516, 1742, 1977, 2080, 2136
BspMAI	CTGCAG	1 cut(s)	2344
BspMI	ACCTGC	2 cut(s)	822, 941
BspPI	GGATC	1 cut(s)	1088
BspQI	GCTCTTC	1 cut(s)	1218
BspT104I	TTCGAA	1 cut(s)	275
BspT107I	GGYRCC	4 cut(s)	310, 317, 1740, 2134
BspTNI	GGTCTC	3 cut(s)	166, 563, 610
BsrDI	GCAATG	1 cut(s)	586
BsrGI	TGTACA	1 cut(s)	417
BsrI	ACTGG	3 cut(s)	592, 1164, 1843
BssMI	GATC	4 cut(s)	1036, 1093, 1468, 2038
BssT1I	CCWWGG	3 cut(s)	150, 265, 1120
Bst2UI	CCWGG	6 cut(s)	288, 315, 938, 1518, 1662, 1815
Bst4CI	ACNGT	6 cut(s)	310, 372, 872, 968, 1570, 1849
Bst6I	CTCTTC	4 cut(s)	180, 1218, 1718, 2246
BstAPI	GCANNNNNTGC	1 cut(s)	2019
BstAUI	TGTACA	1 cut(s)	417
BstBAI	YACGTR	2 cut(s)	920, 2086
BstBI	TTCGAA	1 cut(s)	275
BstC8I	GCNNGC	5 cut(s)	233, 620, 1791, 2094, 2337
BstDEI	CTNAG	5 cut(s)	520, 681, 1176, 1871, 2029
BstDSI	CCRYGG	2 cut(s)	265, 306
BstEII	GGTNACC	1 cut(s)	2263
BstENI	CCTNNNNNAGG	1 cut(s)	1329
BstF5I	GGATG	7 cut(s)	206, 208, 387, 931, 1138, 1923, 2218
BstFNI	CGCG	1 cut(s)	166
BstHHI	GCGC	2 cut(s)	168, 496
BstKTI	GATC	4 cut(s)	1039, 1096, 1471, 2041
BstMAI	GTCTC	4 cut(s)	166, 494, 563, 610
BstMBI	GATC	4 cut(s)	1036, 1093, 1468, 2038
BstMCI	CGRYCG	1 cut(s)	1356
BstNI	CCWGG	6 cut(s)	288, 315, 938, 1518, 1662, 1815
BstNSI	RCATGY	2 cut(s)	81, 1789
BstPI	GGTNACC	1 cut(s)	2263
BstSFI	CTRYAG	1 cut(s)	2340
BstSLI	GKGCMC	1 cut(s)	2137
BstUI	CGCG	1 cut(s)	166
BstXI	CCANNNNNNTGG	3 cut(s)	157, 569, 1243
BsuI	GTATCC	2 cut(s)	700, 1668
BsuRI	GGCC	7 cut(s)	285, 747, 837, 941, 1247, 1793, 2067
BtgI	CCRYGG	2 cut(s)	265, 306
BtsCI	GGATG	7 cut(s)	206, 208, 387, 931, 1138, 1923, 2218
BtsI	GCAGTG	7 cut(s)	342, 546, 627, 763, 1147, 1387, 1452
BveI	ACCTGC	2 cut(s)	822, 941
Cac8I	GCNNGC	5 cut(s)	233, 620, 1791, 2094, 2337
CciI	TCATGA	1 cut(s)	1068
CfoI	GCGC	2 cut(s)	168, 496
Cfr9I	CCCGGG	1 cut(s)	1370
CseI	GACGC	1 cut(s)	1178
Csp6I	GTAC	3 cut(s)	418, 988, 1741
CviQI	GTAC	3 cut(s)	418, 988, 1741
DdeI	CTNAG	5 cut(s)	520, 681, 1176, 1871, 2029
DpnI	GATC	4 cut(s)	1038, 1095, 1470, 2040
DpnII	GATC	4 cut(s)	1036, 1093, 1468, 2038
DraIII	CACNNNGTG	1 cut(s)	2089
EaeI	YGGCCR	1 cut(s)	1245
Eam1104I	CTCTTC	4 cut(s)	180, 1218, 1718, 2246
EarI	CTCTTC	4 cut(s)	180, 1218, 1718, 2246
Ecl136II	GAGCTC	1 cut(s)	1211
Eco130I	CCWWGG	3 cut(s)	150, 265, 1120
Eco24I	GRGCYC	3 cut(s)	1213, 1371, 1872
Eco31I	GGTCTC	3 cut(s)	166, 563, 610
Eco47I	GGWCC	6 cut(s)	142, 187, 252, 514, 1256, 1595
Eco53kI	GAGCTC	1 cut(s)	1211
Eco72I	CACGTG	2 cut(s)	920, 2086
Eco88I	CYCGRG	3 cut(s)	408, 478, 1370
Eco91I	GGTNACC	1 cut(s)	2263
EcoICRI	GAGCTC	1 cut(s)	1211
EcoNI	CCTNNNNNAGG	1 cut(s)	1329
EcoO109I	RGGNCCY	1 cut(s)	514
EcoO65I	GGTNACC	1 cut(s)	2263
EcoRII	CCWGG	6 cut(s)	286, 313, 936, 1516, 1660, 1813
EcoT14I	CCWWGG	3 cut(s)	150, 265, 1120
EcoT22I	ATGCAT	1 cut(s)	208
EcoT38I	GRGCYC	3 cut(s)	1213, 1371, 1872
ErhI	CCWWGG	3 cut(s)	150, 265, 1120
FaqI	GGGAC	4 cut(s)	38, 1207, 1718, 2238
FauI	CCCGC	1 cut(s)	1782
FbaI	TGATCA	2 cut(s)	1036, 1468
FblI	GTMKAC	2 cut(s)	1352, 1758
FokI	GGATG	7 cut(s)	193, 215, 394, 938, 1145, 1910, 2225
FriOI	GRGCYC	3 cut(s)	1213, 1371, 1872
GlaI	GCGC	2 cut(s)	167, 495
GsaI	CCCAGC	2 cut(s)	335, 1722
HaeIII	GGCC	7 cut(s)	285, 747, 837, 941, 1247, 1793, 2067
HapII	CCGG	4 cut(s)	435, 506, 1371, 2268
HgaI	GACGC	1 cut(s)	1178
HhaI	GCGC	2 cut(s)	168, 496
Hin6I	GCGC	2 cut(s)	166, 494
HinP1I	GCGC	2 cut(s)	166, 494
HincII	GTYRAC	4 cut(s)	399, 441, 1006, 1353
HindII	GTYRAC	4 cut(s)	399, 441, 1006, 1353
HindIII	AAGCTT	2 cut(s)	90, 1532
HinfI	GANTC	8 cut(s)	340, 365, 451, 790, 875, 1025, 1642, 1726
HpaII	CCGG	4 cut(s)	435, 506, 1371, 2268
HphI	GGTGA	8 cut(s)	584, 602, 697, 793, 1103, 1981, 2185, 2257
Hpy166II	GTNNAC	8 cut(s)	399, 441, 544, 990, 1006, 1353, 1504, 1759
Hpy188I	TCNGA	5 cut(s)	256, 833, 868, 1098, 1132
Hpy188III	TCNNGA	7 cut(s)	179, 848, 1069, 1526, 1667, 2056, 2101
Hpy8I	GTNNAC	8 cut(s)	399, 441, 544, 990, 1006, 1353, 1504, 1759
Hpy99I	CGWCG	1 cut(s)	2291
HpyAV	CCTTC	6 cut(s)	124, 815, 1481, 1920, 2164, 2240
HpyCH4III	ACNGT	6 cut(s)	310, 372, 872, 968, 1570, 1849
HpyCH4IV	ACGT	5 cut(s)	459, 567, 919, 1278, 2085
HpyF3I	CTNAG	5 cut(s)	520, 681, 1176, 1871, 2029
HpySE526I	ACGT	5 cut(s)	459, 567, 919, 1278, 2085
HspAI	GCGC	2 cut(s)	166, 494
KpnI	GGTACC	1 cut(s)	1744
Ksp22I	TGATCA	2 cut(s)	1036, 1468
Kzo9I	GATC	4 cut(s)	1036, 1093, 1468, 2038
LguI	GCTCTTC	1 cut(s)	1218
LmnI	GCTCC	3 cut(s)	1208, 1540, 2084
LweI	GCATC	8 cut(s)	31, 193, 215, 667, 886, 916, 1543, 1669
MaeII	ACGT	5 cut(s)	459, 567, 919, 1278, 2085
MaeIII	GTNAC	9 cut(s)	80, 473, 590, 685, 1142, 1335, 1862, 1987, 2263
MalI	GATC	4 cut(s)	1038, 1095, 1470, 2040
MboI	GATC	4 cut(s)	1036, 1093, 1468, 2038
MboII	GAAGA	9 cut(s)	16, 167, 1024, 1034, 1205, 1735, 1830, 2188, 2263
MfeI	CAATTG	2 cut(s)	1599, 1970
MhlI	GDGCHC	5 cut(s)	1213, 1371, 1872, 2094, 2137
MlsI	TGGCCA	1 cut(s)	1247
MluNI	TGGCCA	1 cut(s)	1247
MlyI	GAGTC	2 cut(s)	460, 1735
MmeI	TCCRAC	1 cut(s)	214
Mox20I	TGGCCA	1 cut(s)	1247
Mph1103I	ATGCAT	1 cut(s)	208
MscI	TGGCCA	1 cut(s)	1247
MseI	TTAA	2 cut(s)	300, 360
MslI	CAYNNNNRTG	2 cut(s)	675, 1784
Msp20I	TGGCCA	1 cut(s)	1247
MspA1I	CMGCKG	3 cut(s)	1418, 1592, 1718
MspI	CCGG	4 cut(s)	435, 506, 1371, 2268
MunI	CAATTG	2 cut(s)	1599, 1970
MvaI	CCWGG	6 cut(s)	288, 315, 938, 1518, 1662, 1815
MvnI	CGCG	1 cut(s)	166
NciI	CCSGG	5 cut(s)	436, 507, 1371, 1372, 2269
NcoI	CCATGG	1 cut(s)	265
NdeII	GATC	4 cut(s)	1036, 1093, 1468, 2038
NlaIV	GGNNCC	7 cut(s)	312, 319, 516, 1742, 1977, 2080, 2136
NmeAIII	GCCGAG	1 cut(s)	202
NmuCI	GTSAC	8 cut(s)	80, 473, 590, 685, 1142, 1862, 1987, 2263
NsiI	ATGCAT	1 cut(s)	208
NspI	RCATGY	2 cut(s)	81, 1789
NspV	TTCGAA	1 cut(s)	275
PaeR7I	CTCGAG	1 cut(s)	408
PagI	TCATGA	1 cut(s)	1068
PciSI	GCTCTTC	1 cut(s)	1218
PfeI	GAWTC	6 cut(s)	340, 365, 790, 875, 1025, 1642
PflFI	GACNNNGTC	2 cut(s)	158, 2191
PflMI	CCANNNNNTGG	1 cut(s)	2075
PleI	GAGTC	2 cut(s)	459, 1734
PmaCI	CACGTG	2 cut(s)	920, 2086
PmlI	CACGTG	2 cut(s)	920, 2086
PpsI	GAGTC	2 cut(s)	459, 1734
Ppu21I	YACGTR	2 cut(s)	920, 2086
PpuMI	RGGWCCY	1 cut(s)	514
PsiI	TTATAA	1 cut(s)	774
Psp124BI	GAGCTC	1 cut(s)	1213
Psp1406I	AACGTT	1 cut(s)	1278
Psp5II	RGGWCCY	1 cut(s)	514
Psp6I	CCWGG	6 cut(s)	286, 313, 936, 1516, 1660, 1813
PspCI	CACGTG	2 cut(s)	920, 2086
PspEI	GGTNACC	1 cut(s)	2263
PspFI	CCCAGC	2 cut(s)	331, 1718
PspGI	CCWGG	6 cut(s)	286, 313, 936, 1516, 1660, 1813
PspN4I	GGNNCC	7 cut(s)	312, 319, 516, 1742, 1977, 2080, 2136
PspPPI	RGGWCCY	1 cut(s)	514
PspXI	VCTCGAGB	1 cut(s)	408
PstI	CTGCAG	1 cut(s)	2344
PsyI	GACNNNGTC	2 cut(s)	158, 2191
PvuII	CAGCTG	2 cut(s)	1592, 1718
RsaI	GTAC	3 cut(s)	419, 989, 1742
RsaNI	GTAC	3 cut(s)	418, 988, 1741
RseI	CAYNNNNRTG	2 cut(s)	675, 1784
SacI	GAGCTC	1 cut(s)	1213
SalI	GTCGAC	1 cut(s)	1351
SapI	GCTCTTC	1 cut(s)	1218
SaqAI	TTAA	2 cut(s)	300, 360
Sau3AI	GATC	4 cut(s)	1036, 1093, 1468, 2038
SchI	GAGTC	2 cut(s)	460, 1735
SduI	GDGCHC	5 cut(s)	1213, 1371, 1872, 2094, 2137
SfaNI	GCATC	8 cut(s)	31, 193, 215, 667, 886, 916, 1543, 1669
SfcI	CTRYAG	1 cut(s)	2340
Sfr274I	CTCGAG	1 cut(s)	408
SfuI	TTCGAA	1 cut(s)	275
SinI	GGWCC	6 cut(s)	142, 187, 252, 514, 1256, 1595
SlaI	CTCGAG	1 cut(s)	408
SmaI	CCCGGG	1 cut(s)	1372
SmiMI	CAYNNNNRTG	2 cut(s)	675, 1784
SmlI	CTYRAG	4 cut(s)	408, 599, 607, 1689
SmoI	CTYRAG	4 cut(s)	408, 599, 607, 1689
SpeI	ACTAGT	3 cut(s)	196, 323, 1799
SsiI	CCGC	4 cut(s)	348, 1356, 1418, 1789
SstI	GAGCTC	1 cut(s)	1213
StyI	CCWWGG	3 cut(s)	150, 265, 1120
TaaI	ACNGT	6 cut(s)	310, 372, 872, 968, 1570, 1849
TaiI	ACGT	5 cut(s)	462, 570, 922, 1281, 2088
TaqI	TCGA	6 cut(s)	275, 409, 490, 1107, 1352, 1479
TaqII	GACCGA	1 cut(s)	159
TatI	WGTACW	2 cut(s)	417, 987
TfiI	GAWTC	6 cut(s)	340, 365, 790, 875, 1025, 1642
Tru1I	TTAA	2 cut(s)	300, 360
Tru9I	TTAA	2 cut(s)	300, 360
TseFI	GTSAC	8 cut(s)	80, 473, 590, 685, 1142, 1862, 1987, 2263
Tsp45I	GTSAC	8 cut(s)	80, 473, 590, 685, 1142, 1862, 1987, 2263
TspDTI	ATGAA	5 cut(s)	17, 378, 977, 1796, 1885
TspGWI	ACGGA	2 cut(s)	461, 1975
TspMI	CCCGGG	1 cut(s)	1370
Tth111I	GACNNNGTC	2 cut(s)	158, 2191
Van91I	CCANNNNNTGG	1 cut(s)	2075
VpaK11BI	GGWCC	6 cut(s)	142, 187, 252, 514, 1256, 1595
XagI	CCTNNNNNAGG	1 cut(s)	1329
XapI	RAATTY	3 cut(s)	1109, 1240, 1621
XbaI	TCTAGA	1 cut(s)	847
XceI	RCATGY	2 cut(s)	81, 1789
XcmI	CCANNNNNNNNNTGG	1 cut(s)	421
XhoI	CTCGAG	1 cut(s)	408
XmaI	CCCGGG	1 cut(s)	1370
XmiI	GTMKAC	2 cut(s)	1352, 1758
Zsp2I	ATGCAT	1 cut(s)	208

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.