Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0004721

Physical Location & Seq

Genomic Coordinates Reverse (-)

40353142 .. 40366998

Gene Structure 13857 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG238100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7593 bp

ATGGCCAACCTGAGTAGCCTACTCCACGAGCTCCGAGATCGCATAGCAGCCGCTCCATCCCCTCCTCCTAACAACTCATACGACGTCGCTTTGGAGATCCGATTCCGCGCCGTCCTTCCCAACCTTCTTCAAGCTCACGTCGTCCCCTCTCCCTCCGCGAATGAGAGAGAAGTGATAGCTATATTGAGGTTCATCGGTTTCACGGTGAAGTATTTTCCCGGAGTCTTTTACCATGGCAAGGCTGCCGCGATTCTTCCTATGATCGGTCGTATTCTGCCTTTCTTTGCAGAGCCGGCGTTACGTTCTAGGCATGGAGTGATATTTGATACGCTTGGATCGCTTTTAACCTTACTTCGTAGTGGAGCTCAGGATGCATATCGTCAGTTTTTTGTAGATTCCATGCTCGTTGTTCAAGATCTTTTGTGTGTCGCATCAGTCTCTGCAGACATATCAAGTTTTACAGAATCAACTAGAGTGACTTTAAAGTGTTTCTGCGAGTCCTTCAATGCAATTTTTGATGATCCTTCACATCTTGGTGATCTCCCACATAGTATCAAACCTGTTGATGGCACTGGGATCTTGATTAACCTTACAGGAAAAGCAAGGTGGCAACCTTTCGCAACTTGGATGATTAAGCTTCTTGGAAAATGCTTAACTGAAGGAACTCTATATGTGGATGGACTCATCAATATGCCATTTGTTATGGCTGCATGGTCCCTTTTTTGTTATGGGGATGCTGATTTGCACATGGCATTTTTTGAATTTGCACGCATCGTTGGATCTACGATAAAATGTGACGTCATTCCTTATCATAACATAGTCCAGTCGATATCATCTATATTAAGCGAGGACAAAGAGGGGACTCGTGCATTTAGAAACACGGTTTATGATTCGTCCATGGGTGGTTGCCTAGAGGCATTGCACTCTAATTGCCCTGAGGGTATAGTCAAGCTAACAGCTGCACATTTTGTCAATGTATTTCCTCATTCAATGAGGAGAACCCGAAGCCAGGAGCTTAAGGCAAAGCAAGGAAGTACAGTTGAACTTGATATTTCCATCTACCTGGAAGGAATTCACTGTATGTTGCGGTTGCAAAATTGCTTAATCATGAAGTGTATGGACAGTATGCTTGTTGATGCTGATCTTATACATGTCCTTCTAAAGCTTCCATGGACCCATTCTCATGTAGTTACTGACTGCCATCAATTGTGGAAAACAAAATGCATCTCAGTTCAAGTTGCATGCAAACTTGGTCATAGCACAAGAATTGAAACTCAACTTGAAGTATTGGATTTGAGTCTCAATGATGAATGTGACGAAGTTATAATTGAAGCTATAACTTCTATGCCCGTGATTGTCATGTGGTCCGGCCTCAGTGGACTTTCTCACATATTCAGAAGGCTTGAGCTCTTGGGGCGACAGAGAAGTGAGAAGGTTAAGAATATCATTCCCTTTTCTCTTGGTTTTTTGTCATGTCTTTACGGATCTCATAGCAATGGAGATGACATAAATAGAAGTCAGTGTAAACTATTTTTAAAAATGAAAAGTGAGAGACACAGTCAAACAGTGGATTATTTACTGCAAGGATTCTGGTGTTCAAAGTGTGACCGAAGTGTTCAACACAATACTGAGTCATATTCAAATATCATAAATCTACCTGACAAGCCCAGAGAATTGGACTGTAATTTTCTCCATCTTCAGTCTATATATTTTCAGCTTCTTAATGATGAGTCATCTGAAGAGATTCAAGTGGCTTGTGTGAAAAATATCCGCCGAGTTCTTGTACATGGGGGCATAGAGAATCTGTTGGAGTCAAGATTTGAATGGATTAAATGTGTTAAATATCTACTCATTAATACCAAGAAGGCTATAAGAGAAGCATTCTGCACTCAGATTAGTTCATTCGTTGAGGATTCAATTTTGAGTTGCTTATTTCCTGATGAGGGCATGTCAAGTAATGGTAGAGAAGAAGGGTTTTTGAACATAATCAACAGCTCCTTTTCAGAAACTGAAGACCTCCAAATCATTGAGACTCTTTCGGAATCAACAGCTGAAATAATGATTGCAGTTGATATTCAGAGTCAATTATTCTTGTTTTCTCTTCTTTTGTTGGTTGGTCAACTTGATAATCCACACACGACAGTGAGAATGATTGCAGCGAAGTTGATACACAAAGCTTGCTGTTTTCATCTTAAAGGAGGTTTCCAAGTAATCCTTCTGAAAGTTGTTCATGTTCGAAATGAGCTATTTGACTATCTATCTTCGAGCCTCTCTAGCCGTCCGACTATGGTCCAAGAGTTTGCAGAATCAGTTCTTGGTATTGAAATAGAAGAACTTGTCAAGAAAATGGTTCCCGTAGTTCTTCCGAAGCTTGTAGTGTCTCAGCAGGATGATGATCAAGCAGTTGACACCTTATATGAACTGGCCAAGTGGTTAAACACTGATGTTGCAGGTCTTATAGTTCTATGGGTGCCAAAAGTGCTAGCTTTTGCTCTCCATCAACCAGATGTGCGAGAATTAGATGCTGCTGTACAGTTTTACCATACACAAATCAGTTCTAACAAAAGAGAAATCTTTGCTGCTGCATTAAATAATCTTTTAGATGAACTCATATGCTTTCTGGATGGAGGTGATTCAGATGAGATAAGCGGAAGGTTAGCAAGAGTACCTGAGACGATAAAAGAGGTTGCTAGAGTTTTGGATGAAGGTGAAGATCTACCAAACTTTTTGAGGAATCATTTTGCCCACCTTCTTAACAGTATCGATAGAAAGATGCTCCATGCAGAAGAATTTTCATTGCAGAAACAAGCCTTGCAACGAATTGAGATGCTAATTAAAATGATGGGTCGTCATCTTAGTACTTATGTGCCTAAACTAATGGTTCTTCTCATGCATGCTATTGATAAAGACATCCTCCAGAGTGAGGGTCTCTCAGTGTTGCATTTTTTCATTGTGCAGCTGGTGAAAGTATCACCGGCTAGCACTAAACATGTAATTTCACAAGTTTTTGCTGCCCTTATTCCCTTTCTTGAGAGAGATGAAGAAAGTCCAGTACATCTGGATAAAGTGGTAAAAATTTTAGAAGAACTTGTGCTAAATAACAGGGATATTCTAAAGCAGCATATTCGTGAGTTCCCTCCATTGCCCAGTATTCCAGCCTTAATAAAAGTGAACAAGGCTATACAAGAAGCCAGAGGGTCAATGACTTTGAGGGATCGATTGCGGGATGTTGTTGATGGTCTGAATCATGACAGCTTAAATGTGAGATACATGGTTGTGTGTGAGTTGAAAAAGTTGCTGAACCTGGAACGGGAAGGGGTTACATCTCTAATAACTGCTGAAGGTGGTGCGGATATGGAAATTTTGAGCTCTTTAATCAGATCTCTACTTAAAGGATGTGCTGAAGAATCAAGGAGAGCAGTTGGACAGCGGCTAAAGTTGGTGTGTGCTGACTGCCTTGGGGCCCTGGGTGCAGTTGACCCTGCTAAAGTGAAGGCCTTTTCATGCCAACGCTTTAAAATTGAATGCTCTGATGATGACCTTATCTATGAGTTGATTCAAAAGCAACTTGCACGGGCCTTTAGAGCTGCACCTGACACTATAATTCAAGATTCAGCTGTAGTGGCCATACAGGAACTGCTAAAGATTGCTGGTTGTGAGGCCTCATTACATGAGAATGCTGCTGCTTCTATGTTGGCTCCAAAGGACAAAGAGCCTCTGATGGGTTCTGCTTCTGGAATGATGACTACTGATGGTACAAATGAGATGACTAGAAGGGGTCAGAGATTATGGGACCGGTTCTCCAATTATGTGAAAGAAATAATTGCCCCTTATTTGTCCTCTAGATTTCAACTTCCTAATGTTGCTGATCCTGTATTTGTTGGCCCAATTTACCGTCCATCTATGTCATTCAGAAGATGGATATTCTTCTGGATAAGAAAGCTGACTGCACATGCTACTGGTTCTCGTGCAAGAATTTTCAATGCTTGTCGAGGTATAGTGCGCCAGGATATGCAAACAGCAATTTATCTGCTTCCATATTTAGTTCTTAATGCTGTCTGTCATGGCACAGAGGAGGCACGCTATGGCATAACAGAAGAAATCCTATATGTTCTTGATGTTGCTGCATCTCATAACAGTGAGGATGCAGTGCATGGTGTCAATGGTCGGAAAAATGAAGTTTGTGTTCAAGCTGTGTTCATTCTCCTTGATAATCTCGGTCAATGGGTGGATGACGTTAGACAAGAACTATTCCGTTCTTTTCAATTATTAGCTTCTAAGCAACAAATATCCAAGACCAGGCATCAAACTCAGAATACTACCAATCAGGACCAACTTCGTCTACAGTGTGAACATGTTTCAAAACTCTTGTCTGCAGTTCCAAAGGAGACCCTTGCCAGGGCCTCCTTCAGGTGTCAGGCTTATGCAAGATCCTTCATGTATTTTGAGTTGTACGTGAGGGGAAAGTCAGGTTCTTTCAACCCTGCATCTGAGCGAAGTGGTTCATTTGATGATGCAGATGTTTCATATCTGATGGAAATATACAGCTGCCTGGATGAGCCTGATGGTCTTTCTGGATTTGCATTTTTACGTAAATCTTTGGAATTACAAGACCAGCTCTTGATAAACAAAAAAGCCGGGAACTGGGCAGAGGTTTTAACTTCTTGTGAACAAGCTTTGCAGATGGAACCAACATCAGTTCAAAAGCAGTCTGATGTGCTTAACTGTTTATTAAACATGTGTCACCTTCAGGCTATGGTGACTCATGTGGATGGTTTAATGTGTAGGATTCCCCAATACAAGAAAACATGGTGCATGCAAGGTGTGCAGGCAGCATGGAGACTTGGCAGGTGGGATTTGATGGATGAGTACCTTAGTGGAGCTGATCAAGAAGGTTTACTTTGTAGCAGTTCTGAAAGTAATGCGTCATTTGATATGGATGTTGCAAAAATTCTGCAGGCAATGATGAAGAAGGATCAGTTCTCAGTTGAAGAAAAAATTGCCCTCTCCAAGCAAGCTCTAATTGCTCCCCTTTCTGCTGCCGGCATGGATTCTTATGTGCGGGCTTACCCATTTGTCGTGAAACTTCACCTGCTACGAGAGCTTGAGGATTTTTATACTCTTCTTGGTGATGCTTCTTTCTTGGAGAAATCGTTTGCATTTGATGATCCTAAATTCTCAAAAGTGAGGGAGAACTGGGAAAACCGTCTCAGATTTACTCAGCCATCACTCTGGGCAAGAGAGCCACTTTTGGCTTTCCGAAGATTGGTTTTTGCTAACAGTGGTCTTGAGGCTCAAGTTGGGAACTGCTGGCTTCAATATGCAAAGCTCTGTCGCATGGCTGGTCATTATGAGACAGCAAATCAGGCTATTCTAGAAGCCCAAGCTACCAATGCACCTAACGTTCACATGGAGAAGGCTAAACTTCTCTGGAGCACCAGGCGATCTGATGGTGCAATTGCACAGTTGCAACAGTGTCTCTTGAACATGCCTGAAGGGATTGTAGGCTCCAATGCAATTGCATCAATTACCAGTCTTTCACTGGTTCCGCCAAACCTGCCCCCTTTAGTTAATAATACTCAAGCTTGGAATGAAAATCGAGATATTGCAAAGACACTTCTCCTCTATTCTAGGTGGATCCATTACACTGGGCAGAAGCAGAAAGAAGATGTCATAAGTCTATATAGCAGGGTGAGGGAACTGCAGCCCAAGTGGGAGAAAGGATATTTTTACATGGCTAAATATTATGAGGAAGTACTAGCTGATGCCAGGAAGCGTCAGGAAGAAAATCTTGAACTGGGTCCTAGGAAATTACCATCAAATAGTGCTATTGTTGGTTCTTCAAATTTAAATACAGAGAAGGCTTGGTGGGCATATGTGCCTGACATACTGATATTCTATGCAAAAGGGCTTCATCGGGGCCACAAGAATCTCTTTCAGGCACTTCCAAGGTTGTTAACCCTATGGTTTGACTTTGGGACTATGTATGAAAGGAGTGGCACATCATCCAATAAGGATCTCAAAAATGTCCACATGATGGTGATGAGATGCATGCGAGGGTGTTTGAAGGACTTGCCAACATATCAGTGGCTAACAGTACTGCCACAGTTGGTTTCAAGAATCTGCCACCAGAATGAGGAAGTAGTTCGATTGGTCAAAAGCATCATCACATCAGTTCTCCGGCAATACCCACAACAAGCCCTGTGGATTATGGCAGCGGTTTCAAAATCCACTGTTCCATCCAGGAGGGATGCAGCTGCTGAAATTCTACAGGAGGCACGGAAAGGGTTCAGCCAAGGAACTGGTGGAAGCAATCTGTTTGCTCAGTTTGCCAGTCTGATTGATCATTTTATCAAGTTGTGCTTCCATGCTTGTCAGACAAAGGCGAAAACAATCAATGTCTCTACTGAATTTAGTCTCTTAAAGAGGATGATGCCACTGGGAATTATCATGCCAATTCAGCAATCTCTAACTGTTAGCCTACCGACATACGATGGAAAGCTCACTGACTCACTTAAGTCTGATATCTTTTCTGCGACTGATCTTCCTACAATATCAGGAATACGTGATGAGGCTGAGATTCTTTCTTCCCTTCAGCGACCTAAGAAAATTGTTCTATTGGGCAGCGACGGCATTGAACGTCCGTTCCTCTGCAAACCCAAGGATGACCTCAGGAAAGATGCCCGGATGATGGAGTTCACAGCAATGATAAACCGTTTGTTGTCTAAATACCCAGCAAGCCGTCGGAGGAAGCTCTACATTCGCACCTTTGCAGTAATTCCTTTGACAGAGGATTGTGGCATGATAGAATGGGTGCCCCACACACGCGGACTTCGGCATATACTTCAAGACATATATATGAACTGCGGAAACTTTGGTAGTGATACAAATTCAAAGATCAAGCGCTTATATGATCAATGCAAGGGTAAAATACCAGAAGATGAGATGTTGAAGGACAAAATTCTTCCAATGTTCCCTCCAGTTTTCCACAAGTGGTTCCTGACCACTTTTTCTGAGCCAGCTGCTTGGTTTAGGGCACGGATTGCTTATGCACACACTACTGCAGTTTGGTCCATGGTTGGACATATTGTGGGGCTTGGTGACCGACACGGGGAAAACATTCTTTTTGATTCTACAACAGGTGATTGTGTTCATGTTGATTTCAGTTGCTTATTCGATAAAGGCTTGCAGTTGGAGAAGCCTGAACTGGTGCCTTTCAGATTGACTCAGAATATGATTGATGGCTTGGGCATCACTGGTTATGAGGGAATCTTTTTGAGGATCTGCGAAATCACACTTTCAGTACTGAGGATGCATCGGGAGACATTGATGAGTGTTCTTGAGACTTTTGTCCATGATCCTCTTGTAGAATGGACAAAATCACACAAATCTAGTAGAGTAGAAGTTCAGAATCCACACGCACAGCTCGCCATCAGTAACATTGAGGCAAGATTGCAAGGAGTAGTTGTCGGTGTCGGAGCAGCACCATCTTTGCCCTTGGCTGTAGAAGGTCAGGCTCGTCGATTAATAGCTGAAGCAGTCTCCCACAAAAATCTTGGAAAGATGTATATCTGGTGGATGCCATGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000723 GO:0000726 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006139 GO:0006259 GO:0006278 GO:0006281 GO:0006282 GO:0006302 GO:0006303 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010035 GO:0010038 GO:0010044 GO:0010212 GO:0010332 GO:0010833 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031323 GO:0031347 GO:0032200 GO:0032204 GO:0032501 GO:0032504 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043247 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051276 GO:0051321 GO:0051716 GO:0055044 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:1901360 GO:1901362 GO:1901576 GO:2001020

KEGG Pathways

Metabolic & Signaling

ko03460 ko04110 ko04115 ko04214 ko04218 ko05165 ko05166 map03460 map04110 map04115 map04214 map04218 map05165 map05166

Pfam Domains

Protein Families

FAT FATC PI3_PI4_kinase UME

Protein Analysis

2530

Amino Acids

285.75

Weight (kDa)

6.86

Isoelectric Point (pI)

49.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UME	PF08064	909 - 1009	2.6e-16	UME (NUC010) domain
M-HEAT_ATR	PF25030	1296 - 1541	8.3e-33	Serine/threonine-protein kinase ATR, M-HEAT region
FAT	PF02259	1608 - 1987	3.2e-59	FAT domain
HEAT_ATR	PF23593	2014 - 2113	3.9e-32	Serine/threonine-protein kinase ATR-like, HEAT repeats
PI3_PI4_kinase	PF00454	2215 - 2459	5.9e-56	Phosphatidylinositol 3- and 4-kinase
FATC	PF02260	2499 - 2530	9.4e-13	FATC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40820 AT5G40820 AT5G40820 AT5G40820
fragaria_vesca	FvH4_2g15770 FvH4_2g15770 FvH4_2g15770 FvH4_2g15770 FvH4_2g15770
malus_domestica	MD05G1126600.v1.1
prunus_persica	Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1
pyrus_communis	pycom05g12250 pycom10g11100 pycom10g11110
rosa_chinensis	RchiOBHm_Chr6g0279121
rosa_laevigata	RLG00000013166
rosa_multiflora	Rmu_sc0014203.1_g000022
rosa_roxburghii	Rroxscaffold_7G00189110
rosa_rugosa	Rorug06G0122400
rosa_samantha	Rh6AG231100 Rh6BG236000 Rh6CG238100 Rh6DG229700
rosa_wichuraiana	Rw6G020240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1325
AarI	CACCTGC	2 cut(s)	4839, 5100
AatII	GACGTC	2 cut(s)	87, 801
Acc36I	ACCTGC	4 cut(s)	2441, 4839, 5100, 5565
AccB1I	GGYRCC	3 cut(s)	2470, 6814, 7210
AccB7I	CCANNNNNTGG	1 cut(s)	6037
AccBSI	CCGCTC	1 cut(s)	53
AccI	GTMKAC	1 cut(s)	4342
AccII	CGCG	4 cut(s)	108, 158, 248, 6828
AclI	AACGTT	1 cut(s)	5403
AcoI	YGGCCR	3 cut(s)	3, 2424, 3624
AcyI	GRCGYC	2 cut(s)	84, 798
AfeI	AGCGCT	1 cut(s)	6905
AflII	CTTAAG	2 cut(s)	1016, 6515
AflIII	ACRYGT	4 cut(s)	1150, 2989, 4354, 4737
AgeI	ACCGGT	1 cut(s)	3795
AjiI	CACGTC	1 cut(s)	139
AjuI	GAANNNNNNNTTGG	4 cut(s)	2199, 2231, 2298, 2330
AleI	CACNNNNGTG	1 cut(s)	2141
AloI	GAACNNNNNNTCC	2 cut(s)	6630, 6662
Alw21I	GWGCWC	5 cut(s)	33, 367, 1410, 3371, 5438
AlwNI	CAGNNNCTG	4 cut(s)	440, 2009, 3637, 6260
Aor51HI	AGCGCT	1 cut(s)	6905
ApaI	GGGCCC	1 cut(s)	3466
ArsI	GACNNNNNNTTYG	2 cut(s)	4355, 4387
AseI	ATTAAT	2 cut(s)	1854, 7526
AsiGI	ACCGGT	1 cut(s)	3795
Asp700I	GAANNNNTTC	7 cut(s)	1071, 1743, 2310, 4501, 6144, 6287, 7119
AspA2I	CCTAGG	1 cut(s)	5804
AspLEI	GCGC	3 cut(s)	110, 4005, 6906
AsuC2I	CCSGG	3 cut(s)	219, 4639, 6685
AsuII	TTCGAA	1 cut(s)	2236
AsuNHI	GCTAGC	2 cut(s)	2482, 2978
AvaII	GGWCC	8 cut(s)	714, 1173, 1365, 2290, 3793, 4330, 5801, 7071
AvrII	CCTAGG	1 cut(s)	5804
AxyI	CCTNAGG	2 cut(s)	936, 6671
BaeGI	GKGCMC	3 cut(s)	3466, 6819, 7039
BalI	TGGCCA	3 cut(s)	5, 2426, 3626
BamHI	GGATCC	1 cut(s)	5637
BanI	GGYRCC	3 cut(s)	2470, 6814, 7210
BanII	GRGCYC	5 cut(s)	33, 367, 1410, 3371, 3466
BauI	CACGAG	3 cut(s)	26, 864, 3966
BbsI	GAAGAC	1 cut(s)	2019
Bbv12I	GWGCWC	5 cut(s)	33, 367, 1410, 3371, 5438
BceAI	ACGGC	4 cut(s)	95, 2262, 6646, 6726
BcgI	CGANNNNNNTGC	2 cut(s)	3107, 3141
BclI	TGATCA	4 cut(s)	2395, 4885, 6343, 6913
BcnI	CCSGG	3 cut(s)	219, 4639, 6685
BfmI	CTRYAG	9 cut(s)	441, 3618, 4343, 4374, 4955, 5702, 6269, 7062, 7503
BfoI	RGCGCY	1 cut(s)	6907
BfrI	CTTAAG	2 cut(s)	1016, 6515
BfuAI	ACCTGC	4 cut(s)	2441, 4839, 5100, 5565
BglII	AGATCT	3 cut(s)	415, 2713, 3380
BlnI	CCTAGG	1 cut(s)	5804
BmcAI	AGTACT	4 cut(s)	2860, 5757, 6099, 7305
Bme18I	GGWCC	8 cut(s)	714, 1173, 1365, 2290, 3793, 4330, 5801, 7071
BmgBI	CACGTC	1 cut(s)	139
BmrI	ACTGGG	7 cut(s)	582, 3141, 4654, 5206, 5658, 5807, 6449
BmtI	GCTAGC	2 cut(s)	2486, 2982
BmuI	ACTGGG	7 cut(s)	582, 3141, 4654, 5206, 5658, 5807, 6449
BoxI	GACNNNNGTC	2 cut(s)	2288, 7349
BpiI	GAAGAC	1 cut(s)	2019
BplI	GAGNNNNNCTC	2 cut(s)	2915, 2947
BpmI	CTGGAG	3 cut(s)	2900, 5452, 6963
Bpu10I	CCTNAGC	1 cut(s)	366
Bpu14I	TTCGAA	1 cut(s)	2236
BpuEI	CTTGAG	7 cut(s)	1424, 3050, 5126, 5280, 5309, 5565, 7361
BpuMI	CCSGG	3 cut(s)	219, 4639, 6685
Bsa29I	ATCGAT	2 cut(s)	2763, 3217
BsaAI	YACGTR	3 cut(s)	4456, 4592, 6566
BsaBI	GATNNNNATC	4 cut(s)	375, 1140, 2394, 7568
BsaHI	GRCGYC	2 cut(s)	84, 798
BsaI	GGTCTC	2 cut(s)	2933, 4382
BsaWI	WCCGGW	1 cut(s)	3795
BsaXI	ACNNNNNCTCC	6 cut(s)	3785, 3815, 5138, 5168, 7452, 7482
Bse118I	RCCGGY	4 cut(s)	292, 2974, 3795, 5042
Bse21I	CCTNAGG	2 cut(s)	936, 6671
Bse3DI	GCAATG	6 cut(s)	917, 1501, 2795, 3140, 4968, 6711
Bse8I	GATNNNNATC	4 cut(s)	375, 1140, 2394, 7568
BseCI	ATCGAT	2 cut(s)	2763, 3217
BseJI	GATNNNNATC	4 cut(s)	375, 1140, 2394, 7568
BseMI	GCAATG	6 cut(s)	917, 1501, 2795, 3140, 4968, 6711
BseRI	GAGGAG	4 cut(s)	54, 1009, 4088, 5612
BseSI	GKGCMC	3 cut(s)	3466, 6819, 7039
BseYI	CCCAGC	1 cut(s)	6733
BsgI	GTGCAG	7 cut(s)	945, 1870, 2975, 3492, 3573, 3933, 4847
Bsh1236I	CGCG	4 cut(s)	108, 158, 248, 6828
Bsh1285I	CGRYCG	1 cut(s)	268
BshNI	GGYRCC	3 cut(s)	2470, 6814, 7210
BshTI	ACCGGT	1 cut(s)	3795
BshVI	ATCGAT	2 cut(s)	2763, 3217
BsiEI	CGRYCG	1 cut(s)	268
BsiHKAI	GWGCWC	5 cut(s)	33, 367, 1410, 3371, 5438
BsiSI	CCGG	9 cut(s)	219, 293, 1368, 2975, 3796, 4638, 5043, 6181, 6685
BslFI	GGGAC	5 cut(s)	128, 700, 874, 3806, 5992
BsmBI	CGTCTC	2 cut(s)	2666, 5213
BsmFI	GGGAC	5 cut(s)	128, 700, 874, 3806, 5992
BsmI	GAATGC	3 cut(s)	1880, 3530, 3682
Bso31I	GGTCTC	2 cut(s)	2933, 4382
Bsp119I	TTCGAA	1 cut(s)	2236
Bsp120I	GGGCCC	1 cut(s)	3462
Bsp1286I	GDGCHC	8 cut(s)	33, 367, 1410, 3371, 3466, 5438, 6819, 7039
Bsp1407I	TGTACA	2 cut(s)	1783, 2530
Bsp19I	CCATGG	5 cut(s)	232, 897, 1169, 7074, 7583
BspDI	ATCGAT	2 cut(s)	2763, 3217
BspFNI	CGCG	4 cut(s)	108, 158, 248, 6828
BspHI	TCATGA	2 cut(s)	1107, 3247
BspMAI	CTGCAG	5 cut(s)	445, 4378, 4959, 5706, 7066
BspMI	ACCTGC	4 cut(s)	2441, 4839, 5100, 5565
BspOI	GCTAGC	2 cut(s)	2486, 2982
BspT104I	TTCGAA	1 cut(s)	2236
BspT107I	GGYRCC	3 cut(s)	2470, 6814, 7210
BspTI	CTTAAG	2 cut(s)	1016, 6515
BspTNI	GGTCTC	2 cut(s)	2933, 4382
BsrBI	CCGCTC	1 cut(s)	53
BsrDI	GCAATG	6 cut(s)	917, 1501, 2795, 3140, 4968, 6711
BsrFI	RCCGGY	4 cut(s)	292, 2974, 3795, 5042
BsrGI	TGTACA	2 cut(s)	1783, 2530
BssAI	RCCGGY	4 cut(s)	292, 2974, 3795, 5042
BssNI	GRCGYC	2 cut(s)	84, 798
BssSI	CACGAG	3 cut(s)	26, 864, 3966
Bst2BI	CACGAG	3 cut(s)	26, 864, 3966
Bst6I	CTCTTC	3 cut(s)	1734, 2106, 5127
BstACI	GRCGYC	2 cut(s)	84, 798
BstAFI	CTTAAG	2 cut(s)	1016, 6515
BstAPI	GCANNNNNTGC	2 cut(s)	4825, 7043
BstAUI	TGTACA	2 cut(s)	1783, 2530
BstBAI	YACGTR	3 cut(s)	4456, 4592, 6566
BstBI	TTCGAA	1 cut(s)	2236
BstDSI	CCRYGG	5 cut(s)	232, 897, 1169, 7074, 7583
BstEII	GGTNACC	1 cut(s)	7100
BstENI	CCTNNNNNAGG	1 cut(s)	4408
BstFNI	CGCG	4 cut(s)	108, 158, 248, 6828
BstH2I	RGCGCY	1 cut(s)	6907
BstHHI	GCGC	3 cut(s)	110, 4005, 6906
BstMCI	CGRYCG	1 cut(s)	268
BstPAI	GACNNNNGTC	2 cut(s)	2288, 7349
BstPI	GGTNACC	1 cut(s)	7100
BstSFI	CTRYAG	9 cut(s)	441, 3618, 4343, 4374, 4955, 5702, 6269, 7062, 7503
BstSLI	GKGCMC	3 cut(s)	3466, 6819, 7039
BstSNI	TACGTA	1 cut(s)	4592
BstUI	CGCG	4 cut(s)	108, 158, 248, 6828
BstV2I	GAAGAC	1 cut(s)	2019
BstXI	CCANNNNNNTGG	6 cut(s)	1063, 1675, 5232, 5648, 6302, 7026
Bsu15I	ATCGAT	2 cut(s)	2763, 3217
Bsu36I	CCTNAGG	2 cut(s)	936, 6671
BsuTUI	ATCGAT	2 cut(s)	2763, 3217
BtgI	CCRYGG	5 cut(s)	232, 897, 1169, 7074, 7583
BtrI	CACGTC	1 cut(s)	139
BtsI	GCAGTG	1 cut(s)	4155
BveI	ACCTGC	4 cut(s)	2441, 4839, 5100, 5565
CaiI	CAGNNNCTG	4 cut(s)	440, 2009, 3637, 6260
CciI	TCATGA	2 cut(s)	1107, 3247
CfoI	GCGC	3 cut(s)	110, 4005, 6906
Cfr10I	RCCGGY	4 cut(s)	292, 2974, 3795, 5042
ClaI	ATCGAT	2 cut(s)	2763, 3217
CseI	GACGC	2 cut(s)	4914, 5765
CspAI	ACCGGT	1 cut(s)	3795
CspCI	CAANNNNNGTGG	2 cut(s)	6185, 6220
DraI	TTTAAA	4 cut(s)	483, 1536, 3517, 5850
EaeI	YGGCCR	3 cut(s)	3, 2424, 3624
Eam1104I	CTCTTC	3 cut(s)	1734, 2106, 5127
EarI	CTCTTC	3 cut(s)	1734, 2106, 5127
EciI	GGCGGA	2 cut(s)	1760, 5538
Ecl136II	GAGCTC	4 cut(s)	31, 365, 1408, 3369
Eco105I	TACGTA	1 cut(s)	4592
Eco147I	AGGCCT	2 cut(s)	3497, 3662
Eco24I	GRGCYC	5 cut(s)	33, 367, 1410, 3371, 3466
Eco31I	GGTCTC	2 cut(s)	2933, 4382
Eco32I	GATATC	2 cut(s)	831, 6526
Eco47I	GGWCC	8 cut(s)	714, 1173, 1365, 2290, 3793, 4330, 5801, 7071
Eco47III	AGCGCT	1 cut(s)	6905
Eco53kI	GAGCTC	4 cut(s)	31, 365, 1408, 3369
Eco81I	CCTNAGG	2 cut(s)	936, 6671
Eco91I	GGTNACC	1 cut(s)	7100
EcoICRI	GAGCTC	4 cut(s)	31, 365, 1408, 3369
EcoNI	CCTNNNNNAGG	1 cut(s)	4408
EcoO109I	RGGNCCY	4 cut(s)	3462, 3463, 4401, 5801
EcoO65I	GGTNACC	1 cut(s)	7100
EcoRI	GAATTC	1 cut(s)	1071
EcoRV	GATATC	2 cut(s)	831, 6526
EcoT22I	ATGCAT	5 cut(s)	376, 1226, 2895, 6053, 7317
EcoT38I	GRGCYC	5 cut(s)	33, 367, 1410, 3371, 3466
Esp3I	CGTCTC	2 cut(s)	2666, 5213
FaqI	GGGAC	5 cut(s)	128, 700, 874, 3806, 5992
FauI	CCCGC	2 cut(s)	3216, 5055
FauNDI	CATATG	2 cut(s)	2612, 5875
FbaI	TGATCA	4 cut(s)	2395, 4885, 6343, 6913
FblI	GTMKAC	1 cut(s)	4342
FriOI	GRGCYC	5 cut(s)	33, 367, 1410, 3371, 3466
GlaI	GCGC	3 cut(s)	109, 4004, 6905
GsaI	CCCAGC	1 cut(s)	6737
GsuI	CTGGAG	3 cut(s)	2900, 5452, 6963
HaeII	RGCGCY	1 cut(s)	6907
HapII	CCGG	9 cut(s)	219, 293, 1368, 2975, 3796, 4638, 5043, 6181, 6685
HgaI	GACGC	2 cut(s)	4914, 5765
HhaI	GCGC	3 cut(s)	110, 4005, 6906
Hin1I	GRCGYC	2 cut(s)	84, 798
Hin6I	GCGC	3 cut(s)	108, 4003, 6904
HinP1I	GCGC	3 cut(s)	108, 4003, 6904
HincII	GTYRAC	4 cut(s)	2120, 2407, 3478, 5958
HindII	GTYRAC	4 cut(s)	2120, 2407, 3478, 5958
HindIII	AAGCTT	6 cut(s)	635, 1163, 2175, 2369, 4674, 5583
HpaI	GTTAAC	1 cut(s)	5958
HpaII	CCGG	9 cut(s)	219, 293, 1368, 2975, 3796, 4638, 5043, 6181, 6685
Hpy99I	CGWCG	6 cut(s)	86, 89, 143, 6632, 6747, 7524
Hsp92I	GRCGYC	2 cut(s)	84, 798
HspAI	GCGC	3 cut(s)	108, 4003, 6904
KroI	GCCGGC	2 cut(s)	292, 5042
KroNI	GCCGGC	2 cut(s)	294, 5044
Ksp22I	TGATCA	4 cut(s)	2395, 4885, 6343, 6913
KspAI	GTTAAC	1 cut(s)	5958
MbiI	CCGCTC	1 cut(s)	53
MfeI	CAATTG	3 cut(s)	1205, 5457, 5517
MhlI	GDGCHC	8 cut(s)	33, 367, 1410, 3371, 3466, 5438, 6819, 7039
MlsI	TGGCCA	3 cut(s)	5, 2426, 3626
MluNI	TGGCCA	3 cut(s)	5, 2426, 3626
MmeI	TCCRAC	9 cut(s)	757, 1788, 2306, 3403, 4148, 6725, 7060, 7173, 7456
Mox20I	TGGCCA	3 cut(s)	5, 2426, 3626
Mph1103I	ATGCAT	5 cut(s)	376, 1226, 2895, 6053, 7317
MroNI	GCCGGC	2 cut(s)	292, 5042
MroXI	GAANNNNTTC	7 cut(s)	1071, 1743, 2310, 4501, 6144, 6287, 7119
MscI	TGGCCA	3 cut(s)	5, 2426, 3626
Msp20I	TGGCCA	3 cut(s)	5, 2426, 3626
MspA1I	CMGCKG	9 cut(s)	959, 2051, 2959, 3430, 3617, 4548, 6218, 6257, 7022
MspCI	CTTAAG	2 cut(s)	1016, 6515
MspI	CCGG	9 cut(s)	219, 293, 1368, 2975, 3796, 4638, 5043, 6181, 6685
MunI	CAATTG	3 cut(s)	1205, 5457, 5517
Mva1269I	GAATGC	3 cut(s)	1880, 3530, 3682
MvnI	CGCG	4 cut(s)	108, 158, 248, 6828
NaeI	GCCGGC	2 cut(s)	294, 5044
NciI	CCSGG	3 cut(s)	219, 4639, 6685
NcoI	CCATGG	5 cut(s)	232, 897, 1169, 7074, 7583
NdeI	CATATG	2 cut(s)	2612, 5875
NgoMIV	GCCGGC	2 cut(s)	292, 5042
NheI	GCTAGC	2 cut(s)	2482, 2978
NmeAIII	GCCGAG	1 cut(s)	1799
NmuCI	GTSAC	7 cut(s)	475, 794, 1313, 1604, 4742, 4759, 7100
NsiI	ATGCAT	5 cut(s)	376, 1226, 2895, 6053, 7317
NspV	TTCGAA	1 cut(s)	2236
OliI	CACNNNNGTG	1 cut(s)	2141
PaeI	GCATGC	4 cut(s)	1245, 2897, 4819, 6055
PagI	TCATGA	2 cut(s)	1107, 3247
PaqCI	CACCTGC	2 cut(s)	4839, 5100
PasI	CCCWGGG	1 cut(s)	3466
PceI	AGGCCT	2 cut(s)	3497, 3662
PciI	ACATGT	4 cut(s)	1150, 2989, 4354, 4737
PctI	GAATGC	3 cut(s)	1880, 3530, 3682
PdiI	GCCGGC	2 cut(s)	294, 5044
PdmI	GAANNNNTTC	7 cut(s)	1071, 1743, 2310, 4501, 6144, 6287, 7119
PflFI	GACNNNGTC	1 cut(s)	1557
PflMI	CCANNNNNTGG	1 cut(s)	6037
PfoI	TCCNGGA	2 cut(s)	217, 6242
PinAI	ACCGGT	1 cut(s)	3795
Ppu21I	YACGTR	3 cut(s)	4456, 4592, 6566
PpuMI	RGGWCCY	1 cut(s)	5801
PscI	ACATGT	4 cut(s)	1150, 2989, 4354, 4737
PshAI	GACNNNNGTC	2 cut(s)	2288, 7349
PshBI	ATTAAT	2 cut(s)	1854, 7526
PsiI	TTATAA	1 cut(s)	1325
Psp124BI	GAGCTC	4 cut(s)	33, 367, 1410, 3371
Psp1406I	AACGTT	1 cut(s)	5403
Psp5II	RGGWCCY	1 cut(s)	5801
PspEI	GGTNACC	1 cut(s)	7100
PspFI	CCCAGC	1 cut(s)	6733
PspOMI	GGGCCC	1 cut(s)	3462
PspPPI	RGGWCCY	1 cut(s)	5801
PstI	CTGCAG	5 cut(s)	445, 4378, 4959, 5706, 7066
PstNI	CAGNNNCTG	4 cut(s)	440, 2009, 3637, 6260
PsyI	GACNNNGTC	1 cut(s)	1557
PvuII	CAGCTG	7 cut(s)	959, 2051, 2959, 3617, 4548, 6257, 7022
SacI	GAGCTC	4 cut(s)	33, 367, 1410, 3371
ScaI	AGTACT	4 cut(s)	2860, 5757, 6099, 7305
SduI	GDGCHC	8 cut(s)	33, 367, 1410, 3371, 3466, 5438, 6819, 7039
SfcI	CTRYAG	9 cut(s)	441, 3618, 4343, 4374, 4955, 5702, 6269, 7062, 7503
SfuI	TTCGAA	1 cut(s)	2236
SinI	GGWCC	8 cut(s)	714, 1173, 1365, 2290, 3793, 4330, 5801, 7071
SmiI	ATTTAAAT	1 cut(s)	5850
SmlI	CTYRAG	9 cut(s)	1016, 1403, 3029, 5105, 5288, 5295, 5580, 6515, 7340
SmoI	CTYRAG	9 cut(s)	1016, 1403, 3029, 5105, 5288, 5295, 5580, 6515, 7340
SnaBI	TACGTA	1 cut(s)	4592
SphI	GCATGC	4 cut(s)	1245, 2897, 4819, 6055
SseBI	AGGCCT	2 cut(s)	3497, 3662
SspI	AATATT	1 cut(s)	5744
SstI	GAGCTC	4 cut(s)	33, 367, 1410, 3371
StuI	AGGCCT	2 cut(s)	3497, 3662
SwaI	ATTTAAAT	1 cut(s)	5850
TaqII	GACCGA	4 cut(s)	254, 1623, 4208, 7119
TatI	WGTACW	8 cut(s)	1034, 1783, 2530, 2858, 3052, 5755, 6097, 7303
TauI	GCSGC	3 cut(s)	53, 248, 3433
TseFI	GTSAC	7 cut(s)	475, 794, 1313, 1604, 4742, 4759, 7100
Tsp45I	GTSAC	7 cut(s)	475, 794, 1313, 1604, 4742, 4759, 7100
TspGWI	ACGGA	5 cut(s)	1497, 4244, 6295, 6633, 7054
Tth111I	GACNNNGTC	1 cut(s)	1557
Van91I	CCANNNNNTGG	1 cut(s)	6037
Vha464I	CTTAAG	2 cut(s)	1016, 6515
VpaK11BI	GGWCC	8 cut(s)	714, 1173, 1365, 2290, 3793, 4330, 5801, 7071
VspI	ATTAAT	2 cut(s)	1854, 7526
XagI	CCTNNNNNAGG	1 cut(s)	4408
XbaI	TCTAGA	2 cut(s)	3842, 5374
XcmI	CCANNNNNNNNNTGG	4 cut(s)	5539, 6084, 6302, 7553
XmaJI	CCTAGG	1 cut(s)	5804
XmiI	GTMKAC	1 cut(s)	4342
XmnI	GAANNNNTTC	7 cut(s)	1071, 1743, 2310, 4501, 6144, 6287, 7119
ZraI	GACGTC	2 cut(s)	85, 799
ZrmI	AGTACT	4 cut(s)	2860, 5757, 6099, 7305
Zsp2I	ATGCAT	5 cut(s)	376, 1226, 2895, 6053, 7317

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.