Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004721

Physical Location & Seq

Genomic Coordinates Reverse (-)

40504146 .. 40516502

Gene Structure 12357 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G020240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7839 bp

ATGGCCAACCTGAGTAGCCTACTCCACGAGCTCCGAGAACGCATAGCAGCCTCCTCATCCACTCCTCCTAACAACTCACACGACGTCGCTTTGGAGATCCGATTCCGCGCCGTCCTTCCCAACCTTCTTCAAGCTTACGTCGTCCCCTCTCCCTCCGCGGATGAGAGAGAAGTGATAGCTGTATTGAGGTTCATCGGTTACACGGTGAAGCATTTTCCCGGAGTCTTTTACCATGGCAAGGCTGCCGCGATTCTTCCTATGATCGGTCGTATTCTGCCTTTCTTTGCAGAGCCGGCGTTGCGTTCTAGGCATGGAGTGATATTTGATACGCTTGGATCGCTTTTAACCTTACTTCGTAGTGGAGCTCAGGATGCATATCGTCAGTTTTTTGTAGATTCCATGCTCGTTGTTCAAGATCTTTTGTGTGTCGCATCAGTCTCTGCAGACATATCAAGTTTTTCAGAATCAACTAGAGTGACTTTAAAGTGTTTCTGCGAGTCCTTCAATGCAATTTTTGATGATCCTTCACATCTTGGTGATCTCCCACATAGTATCAAACCTGTTGATGGCACTGGGATCTTGATTAACCTTACAGGAAAAGCAAGGTGGCAACCTTTCGCAACTTGGATGATTAAGCTTCTTGGAAAATGCTTAACTGAAGGAACTCTATATGTGGATGGACTCATCAATATGCCATTTGTTATGGCTGCATGGTCCCTTTTTTGTTATGGGGATGCTGATTTGCACATGGCATTTTTTGAATTTGCACGCATCGTTGGATCTGTGATAAAATGTGACGTCATTCCTTATCATAACATAGTCCAGTCGATATCATCTATATTAAGCGAGGACAAAGAGGGGACTCCTGCATTTAGAAACACGGTTTATGATTCGTCCATGGGTGGTTGCCTAGAGGCATTGCACTCTAATTGCCCTGAGGGTGTAGTCAAGCTAACAGCTGCACATTTTGTCAATGTATTTCCTCATTCAATGAGGAGAACCCGAAGCCAGGAGCTTAAGGTTGCATTGTGCAACACATACATACGGATTGCGAAGATTTGCCCCCCTCATATCTGGAGGCCAGAAATTCTCCTATATGTACTTTCATGTCCAGATCCCTGCTTTTCATTGATAGATTGCTTACATGTAGCTCTCTCTATACTTGGTCCTGATCTTGTTGGAGGAAGAATTACAGATAATTCCTGCCAGAGTTTATCCACATTAAGTGATAAATCAATTGAAAACTCACAGGTTGGAGAAAAAAGACCTATTGAGGATGTGGATTCTTTCAACACTAAACGCCAAAAGGTTGATGAAAAAATCATCAATCCTGATTCTAATATTCAGTTGGAATGGCAGTGTGCTCATATAGTTCCTTGTGAGACAGAAGACTATGCAAATCAGATGCATAAATCACTCCTTTCTTTTGCTGAATGTTTAAAATCTCCTGGTGTCTGTCCTAATTCCTTAAGACCTGATCTCGCGTTAATGGCTCTTAGCATTCTTTGCATTGCGTTCTGTAGATATCCTGAGACCAATCTGTCACTATGCATCTTTCATCAGATGTATACATGGATACCTTGGATATGCCAACAGGCAAAGCAAGGAAGTACATTTGAACTTGATATTTCCATCTACCTGGAAGGAATTCACTGTATGTTGCGGTTGCAAAATTGCTTAATCATGAAGTGTATGGACAGTACGCTTGTCGATGCTGATCTTATACATGTCCTTCTAAAGCTTCCATGGACCCATTCTCATGTAGTTACTGACTGCCATCAATTGTGGAAAACAAAATGCATCTCAGTTCAAGTTGCATGCAAACTTGGTCATAGCACAAGAATTGAAACTCAACTTGAAGTATTGGATTTGAGTCTCAATGATGAATGTGACAAAGTTATAATTGAAGCTATAACTTCTATGCCCGTGATTGTCATGTGGTCTGGCCTCAGTGGACTTTCTCACATATTCAGAAGGCTTGAGCTCTTGGGGCGACAGAGAAGTGAGAAGGTTAAGAATATCATTCCCTTTTCTCTTGGTTTTTTGTCATGTCTTTACGGATCTCATAGCAATGGAGATGACATAAATAGAAGTCAGTGTAAACTATTTTTAAAAATGAAAAGTGAGAGACACAGTCAAACAGTGGATTATTTACTGCAAGGATTCTGGTGTTCAAAGTGTGACCGAAGTGTTCAACACAATACTGAGTCATATTCAAATATCATAAATCTACCTGACAAGCCCAGAGAATTGGACTGTAATTTTCTCCATCTTCAGTCTATATATTTTCAGCTTCTTAATGATGAGTCATCTGAAGAGATTCAAGTGGCTTGTGTGAAAAATATCCGCCGAGTTCTTGTACATGGGGGCATAGAGAATCTGTTGGAGTCAAGATTTGAATGGATTACATGTGTTAAATATCTACTCATTAATACCAAGAAGGCTATAAGAGAAGCATTCTGCACTCAGATTATTTTGAGTTGCTTATTTCCTGATGAGGGCATGTCAAGTAATGGTAGAGAAGAAGGGTTTTTGAACATAATCAACAGCTCCTTTTCAGAAACTGAAGACCTCCAAATCATTGAGACTCTTTTGGAATCAACAGCTGAAATAATGATTGCAGTTGATATTCAGAGTCAATTATTCTTGTTTTCTCTTCTTTTGTTGGTTGGTCAACTTGATAATCCACACACGACAGTGAGAATGATTGCAGCGAAGTTGATACACAAAGCTTGCTGTTTTCATCTTAAAGGAGGTTTCCAAGTAATCCTTCTGAAAGTTGTTCATGTTCGAAATGAGCTATTTGACTATCTGTCTTCCAGCCTCTCTAGCCGTCCGACTATGGTCCAAGAGTTTGCAGAATCAGTTCTTGGTATTGAAATAGAAGAACTTCTTGTAGTGTCTCAGCAGGATGATGATCAAGCAGTTGACACCTTATATGAACTGGCCAAGTGGTTAAACACCGATGTTGCAGGTCTTATAGTTCTATGGGTGCCAAAAGTGCTAGCTTTTGCTCTCCATCAACCAGATGTGCGAGAATTAGATGCTGCTGTACAGTTTTACCATACACAAATCAGTTCTAACAAAAGAGAAATCTTTGCTGCTGCATTAAATAATCTTTTAGATGAACTCATATGCTTTCTGGATGGAGGTGATTCAGATGAGATAAGCGGAAGGTTAGCAAGAGTACCTGAGACGATAAAAGAGGTTGCTAGAGTTTTGGATGAAGGTGAAGATCTACCAAACTTTTTGAGGAATCATTTTGCCCACCTTCTTAACAGTATCGATAGAAAGATGCTCCATGCAGAAGAATTTTCATTGCAGAAACAAGCCTTGCAACGAATTGAGATGCTAATTAAAATGATGGGTCGTCATCTTAGTACTTATGTGCCTAAACTAATGGTTCTTCTCATGCATGCTATTGATAAAGACAGCCTCCAGAGTGAGGGTCTCTCAGTGTTGCATTTTTTCATTGTGCAGCTGGTGAAAGTATCACCGGCTAGCACTAAACATGTAATTTCACAAGTTTTTGCTGCCCTTATTCCCTTTCTTGAGAGAGATGAAGAAAGTCCAGTACATCTGGATAAAGTGGTAAAAATTTTAGAAGAACTTGTGCTAAATAACAGGGATATTCTAAAGCAGCATATTCGTGAGTTCCCTCCATTGCCCAGTATTCCAGCCTTAATAAAAGTGAACAAGGCTATACAAGAAGCCAGAGGGTCAATGACTTTGAGGGATCGATTGCGGGATGTTGTTGATGGTCTGAATCATGACAGCTTAAATGTGAGATACATGGTTGTGTGTGAGTTGAAAAAGTTGCTGAACCTGGAACGGGAAGGGGTTACATCTCTAATAACTGCTGAAGGTGGTGCGGATATGGAAATTTTGAGCTCTTTAATCAGATCTCTACTTAAAGGATGTGCTGAAGAATCAAGGAGAGCAGTTGGACAGCGGCTAAAGTTGGTGTGTGCTGACTGCCTTGGGGCCCTGGGTGCAGTTGACCCTGCTAAAGTGAAGGCCTTTTCATGCCAACGCTCTGATGATGACCTTATCTATGAGTTGATTCAAAAGCAACTTGCACGGGCCTTTAGAGCTGCACCTGACACTATAATTCAAGATTCAGCTGTAGTGGCCATACAGGAACTGCTAAAGATTGCTGGTTGTGAGGCCTCATTACATGAGAATGCTGCTGCTTCTATGTTGGCTCCAAAGGACAAAGAGCCTCTGATGGGTTCTGCTTCTGGAATGATGACTACTGATGGTACAAATGAGATGACTAGAAGGGGTCAGAGATTGTGGGACCGATTTCAACTTCCTAATGTTGCTGATCCTGTATTTGTTGGCCCAATTTACCGTCCATCTATGTCATTCAGAAGATGGATATTCTTCTGGATAAGAAAGCTGACTGCACATGCTACTGGTTCTCGTGCAAGAATTTTCAATGCTTGTCGAGGTATAGTGCGCCAGGATATGCAAACAGCAATTTATCTGCTTCCATATTTAGTTCTTAATGCTGTCTGTCATGGCACAGAGGAGGCACGCTATGGCATAACAGAAGAAATCCTATATGTTCTTGATGTTGCTGCTGCATCTCATAACAGTGAGGATGCAGTGCATGGTGTCAATGGTCGGAAAAATGAAGTTTGTGTTCAAGCTGTGTTCATTCTCCTTGATAATCTTGGTCAATGGGTGGATGACGTTACACAAGAACTATTCCGTTCTTTTCAATTATTAGCTTCTAAGCAACAAATATCCAAGACCAGGCATCAAACTCAGAATACTACCAATCAGGACCAACTTCGTCTACAGTGTGAACATGTTTCAAAACTCTTGTCTGCAGTTCCAAAGGAGACCCTTGCCAGGGCCTCCTTCAGGTGTCAGGCTTATGCAAGATCCTTCATGTATTTTGATTGTACTGGTTCATTTGATGATGCAGATGTTTCATATCTGATGGAAATATACAGCTGCCTGGATGAGCCTGATGGTCTTTCTGGATTTGCATTTTTACGTAAATCTTTGGAATTACAAGACCAGCTCTTGATAAACAAAAAAGCCGGGAACTGGGCAGAGGTTTTAACTTCTTGCGAACAAGCTTTGCAGATGGAACCAACATCAGTTCAAAAGCAGTCTGATGTGCTTAACTGTTTATTAAACATGTGTCACCTTCAGGCTATGGTGACTCATGTGGATGGTTTAATGTGTAGGATTCCCCAATACAAGAAAACATGGTGCATGCAAGGTGTGCAGGCAGCATGGAGACTTGGCAGGTGGGATTTGATGGATGAGTACCTTAGTGGAGCTGATCAAGAAGGTTTACTTTGTAGCAGTTCTGAAAGTAATGCGTCATTTGATATGGATGTTGCAAAAATTCTGCAGGCAATGATGAAGAAGGATCAGTTCTCAGTTGAAGAAAAAATTGCCCTCTCCAAGCAAGCTCTAATTGCTCCCCTTTCTGCTGCAGGCATGGATTCTTATGTGCGGGCTTACCCATTTGTCGTGAACTTCACCTGCTACGAGAGCTTGAGGATTTTTATACTCTTCTTGGTGATGCTTCTTTCTTGGAGAAATCGTTTGCATTTGATGATCCTAAATTCTCAAAACCACTTTTGGCTTTCCGAAGATTGGTTATTTGCTAACAGTGGTCTTGAGGCTCAAGTTGGGAACTGCTGGCTTCAATATGCAAAGCTCTGTCGCATGGCTGGTCATTATGAGACAGCAAATCAGGCTATTCTGGAAGCCCAAGCTACCAATGCACCTAACGTTCACATGGAGAAGGCTAAACTTCTCTGGAGCACCAGGCGATCTGATGGTGCAATTGCACAGTTGCAACAGTGCCTCTTGAACATGCCTGAAGGGATTGTAGGCTCCAATGCAATTGCATCAATTACCAGTCTTTCACTGGTTCCGCCAAACCTGCCCCCTTTAGTTATAATACTCAAGCTTGGAATGAAAATCGAGATATTGCAAAGACACTTCTCCTCTATTCTAGGTGGATCCATTACACTGGGCAGAAGCAGAAGAAAGAAGATCGTAAATCTTGAACTGGGTCCTAGGAAATTACCATCAAATAGTGCTATTGTTGGTTCTTCAAATTTAAATACAGAGAAGGCTTGGTGGGCATATGTGCCTGACATACTGATATTCTATGCAAAAGGGCTTCATCGGGGCCACAAGAATCTCTTTCAGGCACTTCCAAGGTTGTTAACCCTATGGTTTGACTTTGGGACTATGTATGAAAGGAGTGGCACATCATCCAATAAGGATCTCAAAAATGTCCACATGATGGTGATGAGATGCATGCGAGGGTGTTTGAAGGACTTGCCAACATATCAGTGGCTAACAGTACTACCACAGTTGGTTTCAAGAATCTGCCACCAGAATGAGGAAGTAGTTCGATTGGTCAAAAGCATCATCACATCAGTTCTCCGGCAATACCCACAACAAGCCCTGTGGATTATGGCAGCGGTTTCAAAATCCACTGTTCCATCGAGGAGGGATGCAGCTGCTGAAATTCTACAGGAGGCACGGAAAGGGTTCAGCCAAGGAACTGGTGGAAGCAATCTGTTTTCTCAGTTTGCCAGTCTGATTGATCATTTTATCAAGTTGTGCTTCCATGCTTGTCAGACAAAGGCGAAAACAATCAATGTCTCTACTGAATTTAGTCTCTTAAAGAGGATGATGCCGCTGGGAATTATCATGCCAATTCAGCAATCTCTAACTGTTAGCCTACCGACATACGATGGGAAGCTCACTGACTCACTTAAGTCTGATATCTTTTCTGCGACTGATCTTCCTACAATATCAGGAATACGTGATGAGGCTGAGATTCTTTCTTCCCTTCAGCGACCTAAGAAAATTGTTCTATTGGGCAGCGACGGCATTGAACGTCCGTTCCTCTGCAAACCCAAGGATGACCTCAGGAAAGATGCCCGGATGATGGAGTTCACAGCAATGATAAACCGTTTGTTGTCTAAATACCCAGCAAGCCGTCGGAGGAAGCTCTACATTCGCACCTTTGCAGTAATTCCTTTGACAGAGGATTGTGGCATGATAGAATGGGTGCCCCACACACGTGGACTTCGGCATATACTTCAAGACATATATATGAACTGCGGAAACTTTGGTATTGATACAAATTCAAAGATCAAGCGCTTATATGATCAATGCAAGGGTAAAATACCAGAAGATGAGATGTTGAAGGACAAAATTCTTCCAATGTTCCCTCCAGTTTTCCACAAGTGGTTCTTGACCACTTTTTCTGAGCCAGCTGCTTGGTTTAGGGCACGGATTGCTTATGCACACACTACTGCAGTTTGGTCCATGGTTGGACATATTGTGGGGCTTGGTGACCGACACGGGGAAAACATTCTTTTTGATTCTACAACAGGTGATTGTGTTCATGTTGATTTCAGTTGCTTATTCGATAAAGGCTTGCAGTTGGAGAAGCCTGAACTGGTGCCTTTCAGATTGACTCAGAATATGATTGATGGCTTGGGCATCACTGGTTATGAGGGAATCTTTTTGAGGATCTGTGAAATCACACTTTCAGTACTGAGGATGCATCGGGAGACATTGATGAGTGTTCTTGAGACTTTTGTCCATGATCCTCTTGTAGAATGGACAAAATCACACAAATCTAGTAGAGTAGAAGTTCAGAATCCACACGCACAGCTAGCCATCAGTAACATTGAGGCAAGATTGCAAGGAGTAGTTGTCGGTGTCGGAGCAGCACCATCTTTGCCCTTGGCTGTAGAAGGTCAGGCTCGTCGATTAATAGCTGAAGCAGTCTCCCACAAAAATCTCGGAAAGATGTATATCTGGTGGATGCCATGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000723 GO:0000726 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005911 GO:0006139 GO:0006259 GO:0006278 GO:0006281 GO:0006282 GO:0006302 GO:0006303 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010035 GO:0010038 GO:0010044 GO:0010212 GO:0010332 GO:0010833 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031323 GO:0031347 GO:0032200 GO:0032204 GO:0032501 GO:0032504 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043247 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051276 GO:0051321 GO:0051716 GO:0055044 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:1901360 GO:1901362 GO:1901576 GO:2001020

KEGG Pathways

Metabolic & Signaling

ko03460 ko04110 ko04115 ko04214 ko04218 ko05165 ko05166 map03460 map04110 map04115 map04214 map04218 map05165 map05166

Pfam Domains

Protein Families

FAT FATC PI3_PI4_kinase UME

Protein Analysis

2612

Amino Acids

294.11

Weight (kDa)

6.59

Isoelectric Point (pI)

49.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UME	PF08064	1083 - 1183	6.4e-17	UME (NUC010) domain
M-HEAT_ATR	PF25030	1449 - 1680	8.2e-28	Serine/threonine-protein kinase ATR, M-HEAT region
FAT	PF02259	1747 - 1952	1.6e-23	FAT domain
HEAT_ATR	PF23593	2096 - 2195	2.7e-32	Serine/threonine-protein kinase ATR-like, HEAT repeats
PI3_PI4_kinase	PF00454	2297 - 2541	3.2e-56	Phosphatidylinositol 3- and 4-kinase
FATC	PF02260	2581 - 2612	9.7e-13	FATC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004721)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40820 AT5G40820 AT5G40820 AT5G40820
fragaria_vesca	FvH4_2g15770 FvH4_2g15770 FvH4_2g15770 FvH4_2g15770 FvH4_2g15770
malus_domestica	MD05G1126600.v1.1
prunus_persica	Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1 Prupe.8G170300_v2.0.a1
pyrus_communis	pycom05g12250 pycom10g11100 pycom10g11110
rosa_chinensis	RchiOBHm_Chr6g0279121
rosa_laevigata	RLG00000013166
rosa_multiflora	Rmu_sc0014203.1_g000022
rosa_roxburghii	Rroxscaffold_7G00189110
rosa_rugosa	Rorug06G0122400
rosa_samantha	Rh6AG231100 Rh6BG236000 Rh6CG238100 Rh6DG229700
rosa_wichuraiana	Rw6G020240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1901, 5930
AarI	CACCTGC	2 cut(s)	5256, 5516
AasI	GACNNNNNNGTC	1 cut(s)	1540
AatII	GACGTC	2 cut(s)	87, 801
Acc36I	ACCTGC	4 cut(s)	2963, 5256, 5516, 5922
AccB1I	GGYRCC	3 cut(s)	2992, 7060, 7456
AccB7I	CCANNNNNTGG	1 cut(s)	6283
AccI	GTMKAC	2 cut(s)	1568, 4804
AccII	CGCG	4 cut(s)	108, 158, 248, 1484
AclI	AACGTT	1 cut(s)	5760
AcoI	YGGCCR	3 cut(s)	3, 2946, 4131
AcvI	CACGTG	1 cut(s)	7073
AcyI	GRCGYC	2 cut(s)	84, 798
AfeI	AGCGCT	1 cut(s)	7151
AflII	CTTAAG	3 cut(s)	1016, 1468, 6761
AflIII	ACRYGT	7 cut(s)	1144, 1726, 2408, 3511, 4816, 5154, 7070
AhdI	GACNNNNNGTC	1 cut(s)	2812
AjuI	GAANNNNNNNTTGG	4 cut(s)	2754, 2786, 2853, 2885
AleI	CACNNNNGTG	2 cut(s)	2696, 7071
AloI	GAACNNNNNNTCC	2 cut(s)	6876, 6908
Alw21I	GWGCWC	6 cut(s)	33, 367, 1366, 1986, 3893, 5795
AlwNI	CAGNNNCTG	4 cut(s)	440, 2564, 4144, 6506
Aor51HI	AGCGCT	1 cut(s)	7151
ApaI	GGGCCC	1 cut(s)	3988
ArsI	GACNNNNNNTTYG	2 cut(s)	4817, 4849
AseI	ATTAAT	2 cut(s)	2430, 7772
Asp700I	GAANNNNTTC	7 cut(s)	1647, 2319, 2865, 2889, 6390, 6533, 7365
AspA2I	CCTAGG	1 cut(s)	6050
AspLEI	GCGC	3 cut(s)	110, 4464, 7152
AsuC2I	CCSGG	3 cut(s)	219, 5056, 6931
AsuII	TTCGAA	1 cut(s)	2791
AsuNHI	GCTAGC	3 cut(s)	3004, 3500, 7672
AvaII	GGWCC	8 cut(s)	714, 1166, 1749, 2845, 4300, 4792, 6047, 7317
AvrII	CCTAGG	1 cut(s)	6050
AxyI	CCTNAGG	2 cut(s)	936, 6917
BaeGI	GKGCMC	3 cut(s)	3988, 7065, 7285
BalI	TGGCCA	3 cut(s)	5, 2948, 4133
BamHI	GGATCC	1 cut(s)	5993
BanI	GGYRCC	3 cut(s)	2992, 7060, 7456
BanII	GRGCYC	5 cut(s)	33, 367, 1986, 3893, 3988
BauI	CACGAG	2 cut(s)	26, 4425
BbrPI	CACGTG	1 cut(s)	7073
BbsI	GAAGAC	3 cut(s)	1395, 2574, 2808
Bbv12I	GWGCWC	6 cut(s)	33, 367, 1366, 1986, 3893, 5795
BceAI	ACGGC	4 cut(s)	95, 2817, 6892, 6972
BcgI	CGANNNNNNTGC	2 cut(s)	3629, 3663
BciVI	GTATCC	1 cut(s)	1569
BclI	TGATCA	4 cut(s)	2917, 5302, 6589, 7159
BcnI	CCSGG	3 cut(s)	219, 5056, 6931
BfoI	RGCGCY	1 cut(s)	7153
BfrI	CTTAAG	3 cut(s)	1016, 1468, 6761
BfuAI	ACCTGC	4 cut(s)	2963, 5256, 5516, 5922
BfuI	GTATCC	1 cut(s)	1569
BglII	AGATCT	3 cut(s)	415, 3235, 3902
BlnI	CCTAGG	1 cut(s)	6050
BmcAI	AGTACT	3 cut(s)	3382, 6345, 7551
Bme18I	GGWCC	8 cut(s)	714, 1166, 1749, 2845, 4300, 4792, 6047, 7317
BmeRI	GACNNNNNGTC	1 cut(s)	2812
BmrI	ACTGGG	5 cut(s)	582, 3663, 5071, 6014, 6053
BmtI	GCTAGC	3 cut(s)	3008, 3504, 7676
BmuI	ACTGGG	5 cut(s)	582, 3663, 5071, 6014, 6053
BoxI	GACNNNNGTC	2 cut(s)	2843, 7595
BpiI	GAAGAC	3 cut(s)	1395, 2574, 2808
BplI	GAGNNNNNCTC	2 cut(s)	3437, 3469
BpmI	CTGGAG	4 cut(s)	1096, 3422, 5809, 7209
Bpu10I	CCTNAGC	1 cut(s)	366
Bpu14I	TTCGAA	1 cut(s)	2791
BpuEI	CTTGAG	7 cut(s)	2000, 3572, 5542, 5637, 5666, 5921, 7607
BpuMI	CCSGG	3 cut(s)	219, 5056, 6931
Bsa29I	ATCGAT	2 cut(s)	3285, 3739
BsaAI	YACGTR	3 cut(s)	5009, 6812, 7073
BsaBI	GATNNNNATC	4 cut(s)	375, 1716, 2916, 7814
BsaHI	GRCGYC	2 cut(s)	84, 798
BsaI	GGTCTC	3 cut(s)	1526, 3455, 4844
BsaXI	ACNNNNNCTCC	4 cut(s)	5554, 5584, 7698, 7728
Bse118I	RCCGGY	2 cut(s)	292, 3496
Bse21I	CCTNAGG	2 cut(s)	936, 6917
Bse3DI	GCAATG	7 cut(s)	917, 1509, 2077, 3317, 3662, 5385, 6957
Bse8I	GATNNNNATC	4 cut(s)	375, 1716, 2916, 7814
BseCI	ATCGAT	2 cut(s)	3285, 3739
BseJI	GATNNNNATC	4 cut(s)	375, 1716, 2916, 7814
BseMI	GCAATG	7 cut(s)	917, 1509, 2077, 3317, 3662, 5385, 6957
BseRI	GAGGAG	6 cut(s)	43, 54, 1009, 4547, 5968, 6505
BseSI	GKGCMC	3 cut(s)	3988, 7065, 7285
BseYI	CCCAGC	2 cut(s)	6685, 6979
BsgI	GTGCAG	7 cut(s)	945, 2446, 3497, 4014, 4080, 4392, 5264
Bsh1236I	CGCG	4 cut(s)	108, 158, 248, 1484
Bsh1285I	CGRYCG	1 cut(s)	268
BshNI	GGYRCC	3 cut(s)	2992, 7060, 7456
BshVI	ATCGAT	2 cut(s)	3285, 3739
BsiEI	CGRYCG	1 cut(s)	268
BsiHKAI	GWGCWC	6 cut(s)	33, 367, 1366, 1986, 3893, 5795
BsiSI	CCGG	6 cut(s)	219, 293, 3497, 5055, 6427, 6931
BslFI	GGGAC	5 cut(s)	128, 700, 874, 4313, 6238
BsmBI	CGTCTC	1 cut(s)	3188
BsmFI	GGGAC	5 cut(s)	128, 700, 874, 4313, 6238
BsmI	GAATGC	3 cut(s)	1500, 2456, 4189
Bso31I	GGTCTC	3 cut(s)	1526, 3455, 4844
Bsp119I	TTCGAA	1 cut(s)	2791
Bsp120I	GGGCCC	1 cut(s)	3984
Bsp1286I	GDGCHC	9 cut(s)	33, 367, 1366, 1986, 3893, 3988, 5795, 7065, 7285
Bsp1407I	TGTACA	2 cut(s)	2359, 3052
Bsp19I	CCATGG	5 cut(s)	232, 897, 1745, 7320, 7829
BspDI	ATCGAT	2 cut(s)	3285, 3739
BspFNI	CGCG	4 cut(s)	108, 158, 248, 1484
BspHI	TCATGA	2 cut(s)	1683, 3769
BspMAI	CTGCAG	5 cut(s)	445, 4840, 5376, 5461, 7312
BspMI	ACCTGC	4 cut(s)	2963, 5256, 5516, 5922
BspOI	GCTAGC	3 cut(s)	3008, 3504, 7676
BspT104I	TTCGAA	1 cut(s)	2791
BspT107I	GGYRCC	3 cut(s)	2992, 7060, 7456
BspTI	CTTAAG	3 cut(s)	1016, 1468, 6761
BspTNI	GGTCTC	3 cut(s)	1526, 3455, 4844
BsrDI	GCAATG	7 cut(s)	917, 1509, 2077, 3317, 3662, 5385, 6957
BsrFI	RCCGGY	2 cut(s)	292, 3496
BsrGI	TGTACA	2 cut(s)	2359, 3052
BssAI	RCCGGY	2 cut(s)	292, 3496
BssNAI	GTATAC	1 cut(s)	1569
BssNI	GRCGYC	2 cut(s)	84, 798
BssSI	CACGAG	2 cut(s)	26, 4425
Bst1107I	GTATAC	1 cut(s)	1569
Bst2BI	CACGAG	2 cut(s)	26, 4425
Bst6I	CTCTTC	3 cut(s)	2310, 2661, 5543
BstACI	GRCGYC	2 cut(s)	84, 798
BstAFI	CTTAAG	3 cut(s)	1016, 1468, 6761
BstAPI	GCANNNNNTGC	3 cut(s)	1506, 5242, 7289
BstAUI	TGTACA	2 cut(s)	2359, 3052
BstBAI	YACGTR	3 cut(s)	5009, 6812, 7073
BstBI	TTCGAA	1 cut(s)	2791
BstDSI	CCRYGG	6 cut(s)	156, 232, 897, 1745, 7320, 7829
BstEII	GGTNACC	1 cut(s)	7346
BstENI	CCTNNNNNAGG	1 cut(s)	4870
BstFNI	CGCG	4 cut(s)	108, 158, 248, 1484
BstH2I	RGCGCY	1 cut(s)	7153
BstHHI	GCGC	3 cut(s)	110, 4464, 7152
BstMCI	CGRYCG	1 cut(s)	268
BstPAI	GACNNNNGTC	2 cut(s)	2843, 7595
BstPI	GGTNACC	1 cut(s)	7346
BstSLI	GKGCMC	3 cut(s)	3988, 7065, 7285
BstSNI	TACGTA	1 cut(s)	5009
BstUI	CGCG	4 cut(s)	108, 158, 248, 1484
BstV2I	GAAGAC	3 cut(s)	1395, 2574, 2808
BstXI	CCANNNNNNTGG	6 cut(s)	1639, 2251, 6004, 6548, 7073, 7272
BstZ17I	GTATAC	1 cut(s)	1569
Bsu15I	ATCGAT	2 cut(s)	3285, 3739
Bsu36I	CCTNAGG	2 cut(s)	936, 6917
BsuI	GTATCC	1 cut(s)	1569
BsuTUI	ATCGAT	2 cut(s)	3285, 3739
BtgI	CCRYGG	6 cut(s)	156, 232, 897, 1745, 7320, 7829
BtsI	GCAGTG	2 cut(s)	1364, 4617
BveI	ACCTGC	4 cut(s)	2963, 5256, 5516, 5922
CaiI	CAGNNNCTG	4 cut(s)	440, 2564, 4144, 6506
CciI	TCATGA	2 cut(s)	1683, 3769
CfoI	GCGC	3 cut(s)	110, 4464, 7152
Cfr10I	RCCGGY	2 cut(s)	292, 3496
Cfr42I	CCGCGG	1 cut(s)	159
ClaI	ATCGAT	2 cut(s)	3285, 3739
CseI	GACGC	1 cut(s)	5331
CspCI	CAANNNNNGTGG	2 cut(s)	6431, 6466
DraI	TTTAAA	4 cut(s)	483, 1440, 2112, 6096
DrdI	GACNNNNNNGTC	1 cut(s)	1540
DriI	GACNNNNNGTC	1 cut(s)	2812
DseDI	GACNNNNNNGTC	1 cut(s)	1540
EaeI	YGGCCR	3 cut(s)	3, 2946, 4131
Eam1104I	CTCTTC	3 cut(s)	2310, 2661, 5543
Eam1105I	GACNNNNNGTC	1 cut(s)	2812
EarI	CTCTTC	3 cut(s)	2310, 2661, 5543
EciI	GGCGGA	2 cut(s)	2336, 5895
Ecl136II	GAGCTC	4 cut(s)	31, 365, 1984, 3891
Eco105I	TACGTA	1 cut(s)	5009
Eco147I	AGGCCT	2 cut(s)	4019, 4169
Eco24I	GRGCYC	5 cut(s)	33, 367, 1986, 3893, 3988
Eco31I	GGTCTC	3 cut(s)	1526, 3455, 4844
Eco32I	GATATC	3 cut(s)	831, 1526, 6772
Eco47I	GGWCC	8 cut(s)	714, 1166, 1749, 2845, 4300, 4792, 6047, 7317
Eco47III	AGCGCT	1 cut(s)	7151
Eco53kI	GAGCTC	4 cut(s)	31, 365, 1984, 3891
Eco72I	CACGTG	1 cut(s)	7073
Eco81I	CCTNAGG	2 cut(s)	936, 6917
Eco91I	GGTNACC	1 cut(s)	7346
EcoICRI	GAGCTC	4 cut(s)	31, 365, 1984, 3891
EcoNI	CCTNNNNNAGG	1 cut(s)	4870
EcoO109I	RGGNCCY	4 cut(s)	3984, 3985, 4863, 6047
EcoO65I	GGTNACC	1 cut(s)	7346
EcoRI	GAATTC	1 cut(s)	1647
EcoRV	GATATC	3 cut(s)	831, 1526, 6772
EcoT22I	ATGCAT	7 cut(s)	376, 1410, 1553, 1802, 3417, 6299, 7563
EcoT38I	GRGCYC	5 cut(s)	33, 367, 1986, 3893, 3988
Esp3I	CGTCTC	1 cut(s)	3188
FaqI	GGGAC	5 cut(s)	128, 700, 874, 4313, 6238
FauI	CCCGC	2 cut(s)	3738, 5472
FauNDI	CATATG	2 cut(s)	3134, 6121
FbaI	TGATCA	4 cut(s)	2917, 5302, 6589, 7159
FblI	GTMKAC	2 cut(s)	1568, 4804
FriOI	GRGCYC	5 cut(s)	33, 367, 1986, 3893, 3988
GlaI	GCGC	3 cut(s)	109, 4463, 7151
GsaI	CCCAGC	2 cut(s)	6689, 6983
GsuI	CTGGAG	4 cut(s)	1096, 3422, 5809, 7209
HaeII	RGCGCY	1 cut(s)	7153
HapII	CCGG	6 cut(s)	219, 293, 3497, 5055, 6427, 6931
HgaI	GACGC	1 cut(s)	5331
HhaI	GCGC	3 cut(s)	110, 4464, 7152
Hin1I	GRCGYC	2 cut(s)	84, 798
Hin6I	GCGC	3 cut(s)	108, 4462, 7150
HinP1I	GCGC	3 cut(s)	108, 4462, 7150
HincII	GTYRAC	4 cut(s)	2675, 2929, 4000, 6204
HindII	GTYRAC	4 cut(s)	2675, 2929, 4000, 6204
HindIII	AAGCTT	6 cut(s)	132, 635, 1739, 2730, 5091, 5939
HpaI	GTTAAC	1 cut(s)	6204
HpaII	CCGG	6 cut(s)	219, 293, 3497, 5055, 6427, 6931
Hpy99I	CGWCG	6 cut(s)	86, 89, 143, 6878, 6993, 7770
HpyCH4IV	ACGT	9 cut(s)	84, 138, 798, 4698, 5008, 5760, 6811, 6886, 7072
HpySE526I	ACGT	9 cut(s)	84, 138, 798, 4698, 5008, 5760, 6811, 6886, 7072
Hsp92I	GRCGYC	2 cut(s)	84, 798
HspAI	GCGC	3 cut(s)	108, 4462, 7150
KroI	GCCGGC	1 cut(s)	292
KroNI	GCCGGC	1 cut(s)	294
Ksp22I	TGATCA	4 cut(s)	2917, 5302, 6589, 7159
KspAI	GTTAAC	1 cut(s)	6204
KspI	CCGCGG	1 cut(s)	159
MaeII	ACGT	9 cut(s)	84, 138, 798, 4698, 5008, 5760, 6811, 6886, 7072
MfeI	CAATTG	4 cut(s)	1236, 1781, 5814, 5874
MhlI	GDGCHC	9 cut(s)	33, 367, 1366, 1986, 3893, 3988, 5795, 7065, 7285
MlsI	TGGCCA	3 cut(s)	5, 2948, 4133
MluNI	TGGCCA	3 cut(s)	5, 2948, 4133
Mox20I	TGGCCA	3 cut(s)	5, 2948, 4133
Mph1103I	ATGCAT	7 cut(s)	376, 1410, 1553, 1802, 3417, 6299, 7563
MroNI	GCCGGC	1 cut(s)	292
MroXI	GAANNNNTTC	7 cut(s)	1647, 2319, 2865, 2889, 6390, 6533, 7365
MscI	TGGCCA	3 cut(s)	5, 2948, 4133
Msp20I	TGGCCA	3 cut(s)	5, 2948, 4133
MspCI	CTTAAG	3 cut(s)	1016, 1468, 6761
MspI	CCGG	6 cut(s)	219, 293, 3497, 5055, 6427, 6931
MunI	CAATTG	4 cut(s)	1236, 1781, 5814, 5874
Mva1269I	GAATGC	3 cut(s)	1500, 2456, 4189
MvnI	CGCG	4 cut(s)	108, 158, 248, 1484
NaeI	GCCGGC	1 cut(s)	294
NciI	CCSGG	3 cut(s)	219, 5056, 6931
NcoI	CCATGG	5 cut(s)	232, 897, 1745, 7320, 7829
NdeI	CATATG	2 cut(s)	3134, 6121
NgoMIV	GCCGGC	1 cut(s)	292
NheI	GCTAGC	3 cut(s)	3004, 3500, 7672
NmeAIII	GCCGAG	1 cut(s)	2375
NmuCI	GTSAC	8 cut(s)	475, 794, 1542, 1889, 2180, 5159, 5176, 7346
NsiI	ATGCAT	7 cut(s)	376, 1410, 1553, 1802, 3417, 6299, 7563
NspV	TTCGAA	1 cut(s)	2791
OliI	CACNNNNGTG	2 cut(s)	2696, 7071
PaeI	GCATGC	4 cut(s)	1821, 3419, 5236, 6301
PagI	TCATGA	2 cut(s)	1683, 3769
PaqCI	CACCTGC	2 cut(s)	5256, 5516
PasI	CCCWGGG	1 cut(s)	3988
PceI	AGGCCT	2 cut(s)	4019, 4169
PciI	ACATGT	6 cut(s)	1144, 1726, 2408, 3511, 4816, 5154
PctI	GAATGC	3 cut(s)	1500, 2456, 4189
PdiI	GCCGGC	1 cut(s)	294
PdmI	GAANNNNTTC	7 cut(s)	1647, 2319, 2865, 2889, 6390, 6533, 7365
PflFI	GACNNNGTC	1 cut(s)	2133
PflMI	CCANNNNNTGG	1 cut(s)	6283
PfoI	TCCNGGA	1 cut(s)	217
PmaCI	CACGTG	1 cut(s)	7073
PmlI	CACGTG	1 cut(s)	7073
Ppu21I	YACGTR	3 cut(s)	5009, 6812, 7073
PpuMI	RGGWCCY	1 cut(s)	6047
PscI	ACATGT	6 cut(s)	1144, 1726, 2408, 3511, 4816, 5154
PshAI	GACNNNNGTC	2 cut(s)	2843, 7595
PshBI	ATTAAT	2 cut(s)	2430, 7772
PsiI	TTATAA	2 cut(s)	1901, 5930
Psp124BI	GAGCTC	4 cut(s)	33, 367, 1986, 3893
Psp1406I	AACGTT	1 cut(s)	5760
Psp5II	RGGWCCY	1 cut(s)	6047
PspCI	CACGTG	1 cut(s)	7073
PspEI	GGTNACC	1 cut(s)	7346
PspFI	CCCAGC	2 cut(s)	6685, 6979
PspOMI	GGGCCC	1 cut(s)	3984
PspPPI	RGGWCCY	1 cut(s)	6047
PstI	CTGCAG	5 cut(s)	445, 4840, 5376, 5461, 7312
PstNI	CAGNNNCTG	4 cut(s)	440, 2564, 4144, 6506
PsyI	GACNNNGTC	1 cut(s)	2133
PvuII	CAGCTG	7 cut(s)	959, 2606, 3481, 4124, 4965, 6503, 7268
SacI	GAGCTC	4 cut(s)	33, 367, 1986, 3893
SacII	CCGCGG	1 cut(s)	159
ScaI	AGTACT	3 cut(s)	3382, 6345, 7551
SduI	GDGCHC	9 cut(s)	33, 367, 1366, 1986, 3893, 3988, 5795, 7065, 7285
Sfr303I	CCGCGG	1 cut(s)	159
SfuI	TTCGAA	1 cut(s)	2791
SgrBI	CCGCGG	1 cut(s)	159
SinI	GGWCC	8 cut(s)	714, 1166, 1749, 2845, 4300, 4792, 6047, 7317
SmiI	ATTTAAAT	1 cut(s)	6096
SnaBI	TACGTA	1 cut(s)	5009
SphI	GCATGC	4 cut(s)	1821, 3419, 5236, 6301
SseBI	AGGCCT	2 cut(s)	4019, 4169
SspI	AATATT	1 cut(s)	1342
SstI	GAGCTC	4 cut(s)	33, 367, 1986, 3893
StuI	AGGCCT	2 cut(s)	4019, 4169
SwaI	ATTTAAAT	1 cut(s)	6096
TaiI	ACGT	9 cut(s)	87, 141, 801, 4701, 5011, 5763, 6814, 6889, 7075
TaqII	GACCGA	4 cut(s)	254, 2199, 4317, 7365
TatI	WGTACW	9 cut(s)	1099, 1610, 2359, 3052, 3380, 3574, 4913, 6343, 7549
TauI	GCSGC	3 cut(s)	248, 3955, 6685
TseFI	GTSAC	8 cut(s)	475, 794, 1542, 1889, 2180, 5159, 5176, 7346
Tsp45I	GTSAC	8 cut(s)	475, 794, 1542, 1889, 2180, 5159, 5176, 7346
TspGWI	ACGGA	6 cut(s)	1060, 2073, 4706, 6541, 6879, 7300
Tth111I	GACNNNGTC	1 cut(s)	2133
Van91I	CCANNNNNTGG	1 cut(s)	6283
Vha464I	CTTAAG	3 cut(s)	1016, 1468, 6761
VpaK11BI	GGWCC	8 cut(s)	714, 1166, 1749, 2845, 4300, 4792, 6047, 7317
VspI	ATTAAT	2 cut(s)	2430, 7772
XagI	CCTNNNNNAGG	1 cut(s)	4870
XcmI	CCANNNNNNNNNTGG	3 cut(s)	5896, 6330, 6548
XmaJI	CCTAGG	1 cut(s)	6050
XmiI	GTMKAC	2 cut(s)	1568, 4804
XmnI	GAANNNNTTC	7 cut(s)	1647, 2319, 2865, 2889, 6390, 6533, 7365
ZraI	GACGTC	2 cut(s)	85, 799
ZrmI	AGTACT	3 cut(s)	3382, 6345, 7551
Zsp2I	ATGCAT	7 cut(s)	376, 1410, 1553, 1802, 3417, 6299, 7563

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.