Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0003813

Physical Location & Seq

Genomic Coordinates Forward (+)

65612126 .. 65621974

Gene Structure 9849 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG488400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3276 bp

ATGCTCACTGATGCAACAACAATGTTAGCAAGTTCAACTGGGGTAGATGCTGTACTTTACCCAGGCAGCTGCTCTGCAGATGCTGCTAATGGTCATGATAAGACGGAGCCAGGTGAAAGACTTATGGAGCATGACCACGTAGAGTGTGCAATAATGGTTACCAGTGAAGCAGAAATAACCGAAATTTGTGAGGAGCATGTCCCTGAGCAGCCTTGTGTTTCTGAATCAGTCTCATGTTCCATATATCCAGAAGGTCTTGAGAATGATGCAATTGACAGCCAACAAAGTGGTGATTTTGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATTAGAACACATACTTCAACATGGTACCCACCCGTGGGCATGGAACATTTAGAAAATGTTGATAGCACATATAAGTCAAATGAAGGTGTTGCTGGAGTAACTCAGATGGATGTTACCACAGACTTAAAGGCAACAGATTTATGTGGTTTGAGTAAGTTTGACTCATTTGAAGAACCAATAAATGTTGATGTTTTACAATATCAACGATATGAAGAGGTTTCAAGGGATGTTGAAATTACTGCTGCCAATACTTTATCGGATACATCCACTTCAACTGTCAGTGTAAGCATATGTCCTGAGCTTCCAGATGAACTTAATCAGAGCAATAACAGGTGCAATGATGGAAATGCATCATGCTTGTCATCAAATGTCCAGGACCACATTGCTAGTTGCAGAAACCAAACCATGGAGCAGGTTGCTGATGAGGTCTGCAACAGTGATTTGCAACCAATTTTTGCAGGAAAAAGTGCTGAACCAGATACTTCTGATCTCTATGATAAACCTAATGAAGTTTACTTTTGTGAGGAAATTCCTAAATATGGTGAACATGATGAGGCGATTCAAACATTAGATACAAGCAGCATGTCCAATTCATGCACTGAAGAAGTTGAGGATTGGAATATGCATTCTGAAACTGGAGCTCCTGCAACAAATGAGTTGGAAATGAAATCTGACCCTGCTTTAGTAAAACGGAAAAAGAAAGTGAGAAGAATTCGAAGTCACAGAAAATTCTTCAATGAGAATGAAGAAGTTCTATTTGAAGGATTGCTGGAGAAGTGTTCCACTGATATTGGTAAATATTGGTCTCAGAGATATCTATTATTCTCCAGATATGATGATGGTATAAAAATGGATGAGGAAGGATGGTTTTCAGTTACTCCAGAAGCTCTAGCTATGCATCATGCCCAACGTTGTGGTAGTGGCATTATCATTGACTGTTTTACTGGAGTTGGTGGTAATGCCATCCAATTTGCTCAGATAAGCAAACATGTAACTGCCATTGATATTGATCCAACAAAGATTGATTATGCTCAACATAATGCTGCTATCTATGGAGTAGACGAAAGGATAGATTTCATAAAGGGGGACTTTTTCCATTTGGCATCAAGGTTGAAGGCAGATACAGTCTTTTTGTCACCACCATGGGGAGGACCTGGTTATGCAAGAGAAGAGACATATGACATTATGACAATGCTTAAGCCACATGATGGATATTTTCTCTTCAACACTGCTAAGCAAGTTGCTTCCAAGATTGTCATGTTTCTCCCAAGAAATGTTGATGTCAACCAATTAGCAGAGTTGTCTCTTTCAGGGACCCCACCATGGTCGCTAGAGAACTCGGCGGCGCACCGTCACCAGCTTGAAGAGAGCTCCAGTGGTCCGGATCGCGATCCAGATCAGAAGACGAAGCCCGCTTTACACATCACCAGCCACCACCACGGTCGTCGATCCAGAAAAGGAGAAAAGGCCAAAGTTCAAAAGCAAGACAACGACGCCGACTTGGTTCTTGATTCAGCACGAAGCTCTTTCTCTCTCGCCTTGAAAGGCAAGCCCCCCAATTTTGTCCACTACAATCCAATCCAAAAACCAAATGACCCCTTGTGTTTTATAGAATGCCAGGACCGTCGATCTAGATCTGAAGCTCTGTCCAAGAAGCTAGACCGGCCGAGACCCGCTTCTTTGGATTTGAACAATGTCACCGCCTCGTCTCCGAGATTGGGGACCTTAAAGAAGAGCTCAATGGCGTCTCGCAAGTCTGGAACCTTCCCAAGTCCCGGAACACCTACTTACCGGCATACTAGTCTTGGAGTGCAGAAGGGTTGGAGCTCGGAGCGAGTTCCGGCCAATGCTAATTTGGGGGGCAGGAAGAATACAACTGCTGCTGCTATGTTGCCATTCAACAATGGGAGGACGTTGCCGTCGAAATGGGAAGATGCAGAGAGGTGGATTTTCAGTCCGGTTCAGGGGCAGTCGAATCAGCAGGCCCAGAGGAGGCCCAAGGCGAAGAGTGGTCCGCTCGGCCCTCCTGGAGTTGCATATTATTCATTGTATTCACCAGCAATGCCGATGTTTGATGGAGGTAGCGTTCCTAATTTCATGGCTGGTTCTCCGTTTTCAGCTGGGGTTATGGCAGCGGATGGAATGGCATTCCACTCCTCCGGTGCTCATAATGGAGCCTTTTCCAATAGGGCTGAGCCTTTCATTGCTCGTTCGGTTAGCGTGCATGGTTGCTCTGAGGTGGTGGACCAGCAGTCATCCCTGCCTGAGTCTCAAGATGAAAAGCTTGATGGGGTTAAGGATGCAGCCACCAATGTTTCTCGTGCAGTTTCACGAAGGGACATGGCAACCCAAATGAGCCCTGATGACAGCATTCACTCGTCTCCCAAGGAGGGACATGATTACTCTGCTTCTACGTCCTCTTTCTTCCCTATTGAGGAAATGCAGAGCGCCCCTTCTTTGAAATTGGAAGTCAGGGATGTGCAGGTAGATGAACGGGTCACTGTGACAAGGTGGTCCAAGAAACATAAAGCCCGAATACCTGGGAAGGGCTCTGATATTGTGGATAGATGGAAAAAGAAAGATGCTGATGTTCGATCTTGTGCTTGGGATCTCTCTGATTCAGCAAAAAACGTTTCAAAAGTTAAAAGAGAGGAAGCTAAAATCGCTGCATGGGAGAATCTGCAGAAGGCAAAAGCTGAGGCAGCAATACGGAAACTAGAGATGAAGCTGGAAAAGAAGAGGTCATCATCCATGGATAAAATTATGAACAAGTTGAGATCAGCTCAGAAAAAAGCTCAAGACATGAGAAGCATAGTGTCAGACAATCAAGCACATGATGGAAGGAGCTCACATAAAACTTTATCAATTCGTAGGACTCATCATATGGGTTCTTTTAGTGGTTGCTTTACGTGTCATGCTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016741 GO:0032259 GO:0034641 GO:0036260 GO:0036261 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071164 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Methyltransf_15 WW

Protein Analysis

1091

Amino Acids

121.09

Weight (kDa)

5.78

Isoelectric Point (pI)

50.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_15	PF09445	426 - 552	7.9e-41	RNA cap guanine-N2 methyltransferase
TRM5-TYW2_MTfase	PF02475	426 - 496	4.5e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_31	PF13847	427 - 529	4.1e-07	Methyltransferase domain
Remorin_C	PF03763	981 - 1079	4.2e-24	Remorin, C-terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45207 AT1G45207
fragaria_vesca	FvH4_2g34300 FvH4_2g34300 FvH4_2g34300 FvH4_2g34300 FvH4_2g34300
malus_domestica	MD00G1220400.v1.1 MD08G1098900.v1.1 MD15G1081400.v1.1
prunus_persica	Prupe.1G434200_v2.0.a1 Prupe.1G434200_v2.0.a1
pyrus_communis	pycom08g08060 pycom15g07610
rosa_chinensis	RchiOBHm_Chr6g0308061
rosa_laevigata	RLG00000010671
rosa_multiflora	Rmu_sc0000765.1_g000041 Rmu_sc0002624.1_g000004
rosa_roxburghii	Rroxscaffold_7G00160610
rosa_rugosa	Rorug01G0143000.1 Rorug01G0143100.1 Rorug05G0563400 Rorug05G0565600 Rorug06G0100400.1 Rorug06G0363500 Rorug06G0363500 Rorug07G0183100
rosa_samantha	Rh1CG259500 Rh3BG268800 Rh6AG472400 Rh6BG483500 Rh6CG488400
rosa_wichuraiana	Rw0G008810 Rw6G041230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	2269, 2863
Acc36I	ACCTGC	2 cut(s)	754, 2824
Acc65I	GGTACC	1 cut(s)	375
AccB1I	GGYRCC	1 cut(s)	375
AccB7I	CCANNNNNTGG	2 cut(s)	757, 1559
AccBSI	CCGCTC	1 cut(s)	2368
AccI	GTMKAC	1 cut(s)	1410
AccII	CGCG	1 cut(s)	1740
AccIII	TCCGGA	1 cut(s)	1732
AciI	CCGC	6 cut(s)	1694, 1764, 2025, 2052, 2366, 2486
AclI	AACGTT	2 cut(s)	1261, 2982
AclWI	GGATC	5 cut(s)	1355, 1736, 1743, 1794, 2967
AcoI	YGGCCR	2 cut(s)	2015, 2193
AcsI	RAATTY	4 cut(s)	183, 879, 1062, 1079
AcuI	CTGAAG	2 cut(s)	972, 2010
AcyI	GRCGYC	2 cut(s)	1845, 2096
AfaI	GTAC	2 cut(s)	54, 377
AflII	CTTAAG	1 cut(s)	1547
AflIII	ACRYGT	2 cut(s)	1339, 3260
AhdI	GACNNNNNGTC	1 cut(s)	2602
AhlI	ACTAGT	1 cut(s)	2150
AjnI	CCWGG	7 cut(s)	61, 109, 723, 1504, 1968, 2377, 2890
AloI	GAACNNNNNNTCC	2 cut(s)	2421, 2453
Alw21I	GWGCWC	6 cut(s)	994, 1724, 2090, 2180, 2518, 3200
AlwI	GGATC	5 cut(s)	1355, 1736, 1743, 1794, 2967
AlwNI	CAGNNNCTG	1 cut(s)	83
Aor13HI	TCCGGA	1 cut(s)	1732
AoxI	GGCC	6 cut(s)	1818, 2015, 2193, 2334, 2345, 2371
ApoI	RAATTY	4 cut(s)	183, 879, 1062, 1079
Asp700I	GAANNNNTTC	1 cut(s)	1101
Asp718I	GGTACC	1 cut(s)	375
AspLEI	GCGC	2 cut(s)	1699, 2801
AsuC2I	CCSGG	1 cut(s)	2127
AsuHPI	GGTGA	8 cut(s)	125, 302, 905, 1479, 1697, 1768, 2041, 2397
AsuII	TTCGAA	1 cut(s)	1066
AvaII	GGWCC	9 cut(s)	727, 1502, 1665, 1730, 1972, 2073, 2363, 2596, 2865
BanI	GGYRCC	1 cut(s)	375
BanII	GRGCYC	7 cut(s)	994, 1724, 2090, 2180, 2711, 2903, 3200
BauI	CACGAG	1 cut(s)	2670
BbsI	GAAGAC	1 cut(s)	1760
Bbv12I	GWGCWC	6 cut(s)	994, 1724, 2090, 2180, 2518, 3200
BbvCI	CCTCAGC	1 cut(s)	3048
BceAI	ACGGC	1 cut(s)	2254
BciT130I	CCWGG	7 cut(s)	63, 111, 725, 1506, 1970, 2379, 2892
BciVI	GTATCC	1 cut(s)	604
BcnI	CCSGG	1 cut(s)	2127
BcuI	ACTAGT	1 cut(s)	2150
BfaI	CTAG	7 cut(s)	738, 1241, 1682, 1983, 2009, 2151, 3068
BfmI	CTRYAG	2 cut(s)	75, 3032
BfoI	RGCGCY	1 cut(s)	2802
BfrI	CTTAAG	1 cut(s)	1547
BfuAI	ACCTGC	2 cut(s)	754, 2824
BfuI	GTATCC	1 cut(s)	604
BglII	AGATCT	1 cut(s)	1985
BlpI	GCTNAGC	2 cut(s)	1584, 2544
Bme1390I	CCNGG	8 cut(s)	63, 111, 725, 1506, 1970, 2127, 2379, 2892
Bme18I	GGWCC	9 cut(s)	727, 1502, 1665, 1730, 1972, 2073, 2363, 2596, 2865
BmeRI	GACNNNNNGTC	1 cut(s)	2602
BmiI	GGNNCC	7 cut(s)	108, 377, 1666, 1667, 2074, 2113, 2527
BmrFI	CCNGG	8 cut(s)	63, 111, 725, 1506, 1970, 2127, 2379, 2892
BmrI	ACTGGG	1 cut(s)	48
BmsI	GCATC	9 cut(s)	37, 70, 256, 710, 1258, 1463, 2275, 2641, 2923
BmuI	ACTGGG	1 cut(s)	48
BpiI	GAAGAC	1 cut(s)	1760
BplI	GAGNNNNNCTC	2 cut(s)	3118, 3150
BpmI	CTGGAG	8 cut(s)	465, 1008, 1142, 1162, 1215, 1317, 1708, 2400
Bpu10I	CCTNAGC	3 cut(s)	204, 648, 3048
Bpu1102I	GCTNAGC	2 cut(s)	1584, 2544
Bpu14I	TTCGAA	1 cut(s)	1066
BpuEI	CTTGAG	3 cut(s)	278, 2607, 3132
BpuMI	CCSGG	1 cut(s)	2127
BsaAI	YACGTR	2 cut(s)	139, 3261
BsaBI	GATNNNNATC	4 cut(s)	1359, 1740, 1746, 1984
BsaHI	GRCGYC	2 cut(s)	1845, 2096
BsaI	GGTCTC	2 cut(s)	1161, 2014
BsaWI	WCCGGW	3 cut(s)	1732, 2308, 2510
BsaXI	ACNNNNNCTCC	4 cut(s)	976, 1006, 2421, 2451
Bse118I	RCCGGY	2 cut(s)	2013, 2142
Bse1I	ACTGG	6 cut(s)	43, 162, 308, 991, 1300, 1725
Bse3DI	GCAATG	4 cut(s)	694, 732, 2418, 2553
Bse8I	GATNNNNATC	4 cut(s)	1359, 1740, 1746, 1984
BseAI	TCCGGA	1 cut(s)	1732
BseBI	CCWGG	7 cut(s)	63, 111, 725, 1506, 1970, 2379, 2892
BseJI	GATNNNNATC	4 cut(s)	1359, 1740, 1746, 1984
BseMI	GCAATG	4 cut(s)	694, 732, 2418, 2553
BseNI	ACTGG	6 cut(s)	43, 162, 308, 991, 1300, 1725
BseRI	GAGGAG	3 cut(s)	206, 2356, 2497
BseX3I	CGGCCG	1 cut(s)	2015
BseYI	CCCAGC	1 cut(s)	2471
BsgI	GTGCAG	3 cut(s)	2183, 2694, 2852
Bsh1236I	CGCG	1 cut(s)	1740
Bsh1285I	CGRYCG	2 cut(s)	1795, 2018
BshFI	GGCC	6 cut(s)	1820, 2017, 2195, 2336, 2347, 2373
BshNI	GGYRCC	1 cut(s)	375
BsiEI	CGRYCG	2 cut(s)	1795, 2018
BsiHKAI	GWGCWC	6 cut(s)	994, 1724, 2090, 2180, 2518, 3200
BsiSI	CCGG	7 cut(s)	1733, 2014, 2127, 2143, 2192, 2309, 2511
BslFI	GGGAC	7 cut(s)	185, 1451, 1678, 2086, 2109, 2702, 2757
BsmBI	CGTCTC	3 cut(s)	2064, 2103, 2736
BsmFI	GGGAC	7 cut(s)	185, 1451, 1678, 2086, 2109, 2702, 2757
BsmI	GAATGC	4 cut(s)	976, 1970, 2498, 2721
BsnI	GGCC	6 cut(s)	1820, 2017, 2195, 2336, 2347, 2373
Bso31I	GGTCTC	2 cut(s)	1161, 2014
Bsp119I	TTCGAA	1 cut(s)	1066
Bsp1286I	GDGCHC	8 cut(s)	994, 1724, 2090, 2180, 2518, 2711, 2903, 3200
Bsp13I	TCCGGA	1 cut(s)	1732
Bsp1720I	GCTNAGC	2 cut(s)	1584, 2544
Bsp19I	CCATGG	4 cut(s)	756, 1493, 1673, 3102
Bsp68I	TCGCGA	1 cut(s)	1740
BspACI	CCGC	6 cut(s)	1694, 1764, 2025, 2052, 2366, 2486
BspANI	GGCC	6 cut(s)	1820, 2017, 2195, 2336, 2347, 2373
BspEI	TCCGGA	1 cut(s)	1732
BspFNI	CGCG	1 cut(s)	1740
BspHI	TCATGA	1 cut(s)	94
BspLI	GGNNCC	7 cut(s)	108, 377, 1666, 1667, 2074, 2113, 2527
BspMAI	CTGCAG	2 cut(s)	79, 3036
BspMI	ACCTGC	2 cut(s)	754, 2824
BspPI	GGATC	5 cut(s)	1355, 1736, 1743, 1794, 2967
BspQI	GCTCTTC	1 cut(s)	2078
BspT104I	TTCGAA	1 cut(s)	1066
BspT107I	GGYRCC	1 cut(s)	375
BspTI	CTTAAG	1 cut(s)	1547
BspTNI	GGTCTC	2 cut(s)	1161, 2014
BsrBI	CCGCTC	1 cut(s)	2368
BsrDI	GCAATG	4 cut(s)	694, 732, 2418, 2553
BsrFI	RCCGGY	2 cut(s)	2013, 2142
BsrI	ACTGG	6 cut(s)	43, 162, 308, 991, 1300, 1725
BssAI	RCCGGY	2 cut(s)	2013, 2142
BssNI	GRCGYC	2 cut(s)	1845, 2096
BssSI	CACGAG	1 cut(s)	2670
BssT1I	CCWWGG	6 cut(s)	756, 1493, 1673, 2349, 2736, 3102
Bst2BI	CACGAG	1 cut(s)	2670
Bst2UI	CCWGG	7 cut(s)	63, 111, 725, 1506, 1970, 2379, 2892
Bst4CI	ACNGT	8 cut(s)	628, 788, 1289, 1477, 1703, 1793, 1976, 2854
Bst6I	CTCTTC	7 cut(s)	558, 1515, 1577, 1710, 2078, 2351, 3083
BstACI	GRCGYC	2 cut(s)	1845, 2096
BstAFI	CTTAAG	1 cut(s)	1547
BstAPI	GCANNNNNTGC	1 cut(s)	83
BstBAI	YACGTR	2 cut(s)	139, 3261
BstBI	TTCGAA	1 cut(s)	1066
BstC8I	GCNNGC	4 cut(s)	1764, 1901, 2334, 2573
BstDSI	CCRYGG	6 cut(s)	384, 756, 1493, 1673, 1789, 3102
BstEII	GGTNACC	1 cut(s)	157
BstFNI	CGCG	1 cut(s)	1740
BstH2I	RGCGCY	1 cut(s)	2802
BstHHI	GCGC	2 cut(s)	1699, 2801
BstMCI	CGRYCG	2 cut(s)	1795, 2018
BstMWI	GCNNNNNNNGC	4 cut(s)	83, 2014, 3014, 3053
BstNI	CCWGG	7 cut(s)	63, 111, 725, 1506, 1970, 2379, 2892
BstNSI	RCATGY	3 cut(s)	200, 937, 1343
BstPI	GGTNACC	1 cut(s)	157
BstSCI	CCNGG	8 cut(s)	61, 109, 723, 1504, 1968, 2125, 2377, 2890
BstSFI	CTRYAG	2 cut(s)	75, 3032
BstUI	CGCG	1 cut(s)	1740
BstV2I	GAAGAC	1 cut(s)	1760
BstX2I	RGATCY	2 cut(s)	1985, 2959
BstXI	CCANNNNNNTGG	2 cut(s)	287, 1265
BstYI	RGATCY	2 cut(s)	1985, 2959
BstZI	CGGCCG	1 cut(s)	2015
BsuI	GTATCC	1 cut(s)	604
BsuRI	GGCC	6 cut(s)	1820, 2017, 2195, 2336, 2347, 2373
BtgI	CCRYGG	6 cut(s)	384, 756, 1493, 1673, 1789, 3102
BtsI	GCAGTG	1 cut(s)	1578
BtsIMutI	CAGTG	9 cut(s)	6, 169, 637, 793, 948, 1134, 1578, 1732, 2850
BtuMI	TCGCGA	1 cut(s)	1740
BveI	ACCTGC	2 cut(s)	754, 2824
Cac8I	GCNNGC	4 cut(s)	1764, 1901, 2334, 2573
CaiI	CAGNNNCTG	1 cut(s)	83
CciI	TCATGA	1 cut(s)	94
CfoI	GCGC	2 cut(s)	1699, 2801
Cfr10I	RCCGGY	2 cut(s)	2013, 2142
CseI	GACGC	2 cut(s)	1853, 2085
CsiI	ACCWGGT	1 cut(s)	1504
Csp6I	GTAC	2 cut(s)	53, 376
CspCI	CAANNNNNGTGG	2 cut(s)	1123, 1158
CviQI	GTAC	2 cut(s)	53, 376
DrdI	GACNNNNNNGTC	2 cut(s)	2269, 2863
DriI	GACNNNNNGTC	1 cut(s)	2602
DseDI	GACNNNNNNGTC	2 cut(s)	2269, 2863
EaeI	YGGCCR	2 cut(s)	2015, 2193
EagI	CGGCCG	1 cut(s)	2015
Eam1104I	CTCTTC	7 cut(s)	558, 1515, 1577, 1710, 2078, 2351, 3083
Eam1105I	GACNNNNNGTC	1 cut(s)	2602
EarI	CTCTTC	7 cut(s)	558, 1515, 1577, 1710, 2078, 2351, 3083
Ecl136II	GAGCTC	5 cut(s)	992, 1722, 2088, 2178, 3198
EclXI	CGGCCG	1 cut(s)	2015
Eco130I	CCWWGG	6 cut(s)	756, 1493, 1673, 2349, 2736, 3102
Eco24I	GRGCYC	7 cut(s)	994, 1724, 2090, 2180, 2711, 2903, 3200
Eco31I	GGTCTC	2 cut(s)	1161, 2014
Eco32I	GATATC	1 cut(s)	1166
Eco47I	GGWCC	9 cut(s)	727, 1502, 1665, 1730, 1972, 2073, 2363, 2596, 2865
Eco52I	CGGCCG	1 cut(s)	2015
Eco53kI	GAGCTC	5 cut(s)	992, 1722, 2088, 2178, 3198
Eco57I	CTGAAG	2 cut(s)	972, 2010
Eco91I	GGTNACC	1 cut(s)	157
EcoICRI	GAGCTC	5 cut(s)	992, 1722, 2088, 2178, 3198
EcoO109I	RGGNCCY	3 cut(s)	1502, 1665, 2073
EcoO65I	GGTNACC	1 cut(s)	157
EcoRI	GAATTC	1 cut(s)	1062
EcoRII	CCWGG	7 cut(s)	61, 109, 723, 1504, 1968, 2377, 2890
EcoRV	GATATC	1 cut(s)	1166
EcoT14I	CCWWGG	6 cut(s)	756, 1493, 1673, 2349, 2736, 3102
EcoT22I	ATGCAT	3 cut(s)	703, 978, 1251
EcoT38I	GRGCYC	7 cut(s)	994, 1724, 2090, 2180, 2711, 2903, 3200
ErhI	CCWWGG	6 cut(s)	756, 1493, 1673, 2349, 2736, 3102
Esp3I	CGTCTC	3 cut(s)	2064, 2103, 2736
FalI	AAGNNNNNCTT	2 cut(s)	1424, 1456
FaqI	GGGAC	7 cut(s)	185, 1451, 1678, 2086, 2109, 2702, 2757
FauI	CCCGC	2 cut(s)	1771, 2032
FauNDI	CATATG	3 cut(s)	641, 1528, 3234
FblI	GTMKAC	1 cut(s)	1410
FriOI	GRGCYC	7 cut(s)	994, 1724, 2090, 2180, 2711, 2903, 3200
FspBI	CTAG	7 cut(s)	738, 1241, 1682, 1983, 2009, 2151, 3068
GlaI	GCGC	2 cut(s)	1698, 2800
GsaI	CCCAGC	1 cut(s)	2475
GsuI	CTGGAG	8 cut(s)	465, 1008, 1142, 1162, 1215, 1317, 1708, 2400
HaeII	RGCGCY	1 cut(s)	2802
HaeIII	GGCC	6 cut(s)	1820, 2017, 2195, 2336, 2347, 2373
HapII	CCGG	7 cut(s)	1733, 2014, 2127, 2143, 2192, 2309, 2511
HgaI	GACGC	2 cut(s)	1853, 2085
HhaI	GCGC	2 cut(s)	1699, 2801
Hin1I	GRCGYC	2 cut(s)	1845, 2096
Hin6I	GCGC	2 cut(s)	1697, 2799
HinP1I	GCGC	2 cut(s)	1697, 2799
HincII	GTYRAC	1 cut(s)	1636
HindII	GTYRAC	1 cut(s)	1636
HindIII	AAGCTT	1 cut(s)	2633
HpaII	CCGG	7 cut(s)	1733, 2014, 2127, 2143, 2192, 2309, 2511
HphI	GGTGA	8 cut(s)	125, 302, 905, 1479, 1697, 1768, 2041, 2397
Hpy166II	GTNNAC	6 cut(s)	865, 896, 1411, 1636, 1918, 2596
Hpy8I	GTNNAC	6 cut(s)	865, 896, 1411, 1636, 1918, 2596
Hpy99I	CGWCG	4 cut(s)	1800, 1847, 1980, 2275
HpyCH4III	ACNGT	8 cut(s)	628, 788, 1289, 1477, 1703, 1793, 1976, 2854
HpyCH4IV	ACGT	6 cut(s)	138, 1261, 2264, 2765, 2982, 3260
HpyF10VI	GCNNNNNNNGC	4 cut(s)	83, 2014, 3014, 3053
HpySE526I	ACGT	6 cut(s)	138, 1261, 2264, 2765, 2982, 3260
Hsp92I	GRCGYC	2 cut(s)	1845, 2096
HspAI	GCGC	2 cut(s)	1697, 2799
KflI	GGGWCCC	1 cut(s)	1665
Kpn2I	TCCGGA	1 cut(s)	1732
KpnI	GGTACC	1 cut(s)	379
LguI	GCTCTTC	1 cut(s)	2078
LweI	GCATC	9 cut(s)	37, 70, 256, 710, 1258, 1463, 2275, 2641, 2923
MabI	ACCWGGT	1 cut(s)	1504
MaeI	CTAG	7 cut(s)	738, 1241, 1682, 1983, 2009, 2151, 3068
MaeII	ACGT	6 cut(s)	138, 1261, 2264, 2765, 2982, 3260
MbiI	CCGCTC	1 cut(s)	2368
MfeI	CAATTG	1 cut(s)	270
MflI	RGATCY	2 cut(s)	1985, 2959
MhlI	GDGCHC	8 cut(s)	994, 1724, 2090, 2180, 2518, 2711, 2903, 3200
MlyI	GAGTC	3 cut(s)	506, 2627, 3220
MmeI	TCCRAC	3 cut(s)	990, 1388, 2153
Mph1103I	ATGCAT	3 cut(s)	703, 978, 1251
MroI	TCCGGA	1 cut(s)	1732
MroXI	GAANNNNTTC	1 cut(s)	1101
MseI	TTAA	6 cut(s)	476, 666, 1548, 2078, 2646, 2994
MslI	CAYNNNNRTG	1 cut(s)	1492
MspA1I	CMGCKG	3 cut(s)	69, 2471, 2486
MspCI	CTTAAG	1 cut(s)	1547
MspI	CCGG	7 cut(s)	1733, 2014, 2127, 2143, 2192, 2309, 2511
MspR9I	CCNGG	8 cut(s)	63, 111, 725, 1506, 1970, 2127, 2379, 2892
MunI	CAATTG	1 cut(s)	270
Mva1269I	GAATGC	4 cut(s)	976, 1970, 2498, 2721
MvaI	CCWGG	7 cut(s)	63, 111, 725, 1506, 1970, 2379, 2892
MvnI	CGCG	1 cut(s)	1740
MwoI	GCNNNNNNNGC	4 cut(s)	83, 2014, 3014, 3053
NciI	CCSGG	1 cut(s)	2127
NcoI	CCATGG	4 cut(s)	756, 1493, 1673, 3102
NdeI	CATATG	3 cut(s)	641, 1528, 3234
NlaIV	GGNNCC	7 cut(s)	108, 377, 1666, 1667, 2074, 2113, 2527
NmeAIII	GCCGAG	3 cut(s)	1670, 2043, 2349
NmuCI	GTSAC	6 cut(s)	1070, 1485, 1703, 2047, 2848, 2854
NruI	TCGCGA	1 cut(s)	1740
NsiI	ATGCAT	3 cut(s)	703, 978, 1251
NspI	RCATGY	3 cut(s)	200, 937, 1343
NspV	TTCGAA	1 cut(s)	1066
PagI	TCATGA	1 cut(s)	94
PciI	ACATGT	1 cut(s)	1339
PciSI	GCTCTTC	1 cut(s)	2078
PcsI	WCGNNNNNNNCGW	1 cut(s)	2270
PctI	GAATGC	4 cut(s)	976, 1970, 2498, 2721
PdmI	GAANNNNTTC	1 cut(s)	1101
PfeI	GAWTC	7 cut(s)	224, 320, 910, 1862, 2326, 2969, 3028
PflMI	CCANNNNNTGG	2 cut(s)	757, 1559
PfoI	TCCNGGA	3 cut(s)	723, 2125, 2377
PleI	GAGTC	3 cut(s)	506, 2626, 3220
PpsI	GAGTC	3 cut(s)	506, 2626, 3220
Ppu21I	YACGTR	2 cut(s)	139, 3261
PpuMI	RGGWCCY	3 cut(s)	1502, 1665, 2073
PscI	ACATGT	1 cut(s)	1339
Psp124BI	GAGCTC	5 cut(s)	994, 1724, 2090, 2180, 3200
Psp1406I	AACGTT	2 cut(s)	1261, 2982
Psp5II	RGGWCCY	3 cut(s)	1502, 1665, 2073
Psp6I	CCWGG	7 cut(s)	61, 109, 723, 1504, 1968, 2377, 2890
PspEI	GGTNACC	1 cut(s)	157
PspFI	CCCAGC	1 cut(s)	2471
PspGI	CCWGG	7 cut(s)	61, 109, 723, 1504, 1968, 2377, 2890
PspN4I	GGNNCC	7 cut(s)	108, 377, 1666, 1667, 2074, 2113, 2527
PspPPI	RGGWCCY	3 cut(s)	1502, 1665, 2073
PstI	CTGCAG	2 cut(s)	79, 3036
PstNI	CAGNNNCTG	1 cut(s)	83
PsuI	RGATCY	2 cut(s)	1985, 2959
PvuII	CAGCTG	2 cut(s)	69, 2471
RruI	TCGCGA	1 cut(s)	1740
RsaI	GTAC	2 cut(s)	54, 377
RsaNI	GTAC	2 cut(s)	53, 376
RseI	CAYNNNNRTG	1 cut(s)	1492
SacI	GAGCTC	5 cut(s)	994, 1724, 2090, 2180, 3200
SapI	GCTCTTC	1 cut(s)	2078
SaqAI	TTAA	6 cut(s)	476, 666, 1548, 2078, 2646, 2994
SchI	GAGTC	3 cut(s)	506, 2627, 3220
ScrFI	CCNGG	8 cut(s)	63, 111, 725, 1506, 1970, 2127, 2379, 2892
SduI	GDGCHC	8 cut(s)	994, 1724, 2090, 2180, 2518, 2711, 2903, 3200
SexAI	ACCWGGT	1 cut(s)	1504
SfaNI	GCATC	9 cut(s)	37, 70, 256, 710, 1258, 1463, 2275, 2641, 2923
SfcI	CTRYAG	2 cut(s)	75, 3032
SfuI	TTCGAA	1 cut(s)	1066
SinI	GGWCC	9 cut(s)	727, 1502, 1665, 1730, 1972, 2073, 2363, 2596, 2865
SmiMI	CAYNNNNRTG	1 cut(s)	1492
SmlI	CTYRAG	4 cut(s)	257, 1547, 2622, 3147
SmoI	CTYRAG	4 cut(s)	257, 1547, 2622, 3147
SpeI	ACTAGT	1 cut(s)	2150
SsiI	CCGC	6 cut(s)	1694, 1764, 2025, 2052, 2366, 2486
SspI	AATATT	2 cut(s)	352, 1151
SspMI	CTAG	7 cut(s)	738, 1241, 1682, 1983, 2009, 2151, 3068
SstI	GAGCTC	5 cut(s)	994, 1724, 2090, 2180, 3200
StyD4I	CCNGG	8 cut(s)	61, 109, 723, 1504, 1968, 2125, 2377, 2890
StyI	CCWWGG	6 cut(s)	756, 1493, 1673, 2349, 2736, 3102
TaaI	ACNGT	8 cut(s)	628, 788, 1289, 1477, 1703, 1793, 1976, 2854
TaiI	ACGT	6 cut(s)	141, 1264, 2267, 2768, 2985, 3263
TaqI	TCGA	6 cut(s)	1066, 1798, 1978, 2273, 2324, 2944
TatI	WGTACW	1 cut(s)	52
TauI	GCSGC	1 cut(s)	1697
TfiI	GAWTC	7 cut(s)	224, 320, 910, 1862, 2326, 2969, 3028
Tru1I	TTAA	6 cut(s)	476, 666, 1548, 2078, 2646, 2994
Tru9I	TTAA	6 cut(s)	476, 666, 1548, 2078, 2646, 2994
TscAI	CASTG	9 cut(s)	13, 169, 637, 793, 955, 1141, 1585, 1732, 2857
TseFI	GTSAC	6 cut(s)	1070, 1485, 1703, 2047, 2848, 2854
Tsp45I	GTSAC	6 cut(s)	1070, 1485, 1703, 2047, 2848, 2854
TspGWI	ACGGA	4 cut(s)	119, 1057, 2451, 3076
TspRI	CASTG	9 cut(s)	13, 169, 637, 793, 955, 1141, 1585, 1732, 2857
Van91I	CCANNNNNTGG	2 cut(s)	757, 1559
Vha464I	CTTAAG	1 cut(s)	1547
VpaK11BI	GGWCC	9 cut(s)	727, 1502, 1665, 1730, 1972, 2073, 2363, 2596, 2865
XapI	RAATTY	4 cut(s)	183, 879, 1062, 1079
XbaI	TCTAGA	1 cut(s)	1982
XceI	RCATGY	3 cut(s)	200, 937, 1343
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2203
XmiI	GTMKAC	1 cut(s)	1410
XmnI	GAANNNNTTC	1 cut(s)	1101
XspI	CTAG	7 cut(s)	738, 1241, 1682, 1983, 2009, 2151, 3068
Zsp2I	ATGCAT	3 cut(s)	703, 978, 1251

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.