Rh6DG069000

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013909
Physical Location & Seq
Reverse (-)
7227495 .. 7237217
9723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG069000.1

Sequence Viewer

Length: 570 bp
ATGATGGGCTCAAACTCAATCCAAATCCCAATCGCTCTGGCCTATCACACCTCCCCTCCTTCTTCTCTTTCACCTCTCACCTTCTCCCTTCCTCTACTTCCTCGCTCGCCTTTACCTCCCTCTCTACGACGTCGTTTCCAGACTCTCCGCCTCGCAATCCATCGCCGCTCTTCAATTCTCGCCTCCTCCTCCTCCGCGGCCGGCGTCACGAATTCCGCGCCGAGAAATGGAAACTACACCGTCAGCGATTTCATGACCACCAAAGAGCATTTACACGTCGTTAAGCCCTCCACAACCGTCGACCAAGCATTGGATTTTCTGGTGGAGAAGAGAATTACCGGATTTCCTGTGATTGATGATGACTGGAAGCTGGTTGGTGTTGTTTCGGATTATGACTTGTTAGCATTGGACTCCATATCAGGAAATATAGGAGGTATGGGTGTGTTGCAAGATCTGAATTTTTATACTTTCGTTTGCCTTTTCAGTTCTGTTATATGGTGTGCTTATGATGTGTTGGTTAAGAAGTTATGGTTTGTTATGTTAAGACAATTTGTCATGTGTAGAATTTAG

Protein Analysis

189

Amino Acids

21.0

Weight (kDa)

9.12

Isoelectric Point (pI)

60.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBS PF00571 81 - 135 3.6e-13 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013909)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34120
fragaria_vesca FvH4_2g05710 FvH4_2g05710
malus_domestica MD05G1085200.v1.1
prunus_persica Prupe.8G129500_v2.0.a1
pyrus_communis pycom05g08450 pycom1609g00040
rosa_chinensis RchiOBHm_Chr6g0256581
rosa_laevigata RLG00000014753
rosa_multiflora Rmu_sc0003012.1_g000012
rosa_roxburghii Rroxscaffold_7G00209210
rosa_rugosa Rorug05G0564700
rosa_samantha Rh6AG082500 Rh6BG075200 Rh6CG071800 Rh6DG069000
rosa_wichuraiana Rw6G007430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 133
AccB7I CCANNNNNTGG 1 cut(s) 310
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 300
AccII CGCG 2 cut(s) 197, 218
AciI CCGC 5 cut(s) 148, 166, 195, 197, 216
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 3 cut(s) 211, 457, 564
AcyI GRCGYC 2 cut(s) 130, 204
AfiI CCNNNNNNNGG 2 cut(s) 227, 310
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 1 cut(s) 174
AhdI GACNNNNNGTC 1 cut(s) 551
AjiI CACGTC 1 cut(s) 277
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
AoxI GGCC 2 cut(s) 39, 198
ApoI RAATTY 3 cut(s) 211, 457, 564
AspLEI GCGC 1 cut(s) 220
AsuHPI GGTGA 2 cut(s) 63, 70
BanII GRGCYC 1 cut(s) 11
BccI CCATC 1 cut(s) 168
BglII AGATCT 1 cut(s) 451
BisI GCNGC 2 cut(s) 166, 198
BlsI GCNGC 2 cut(s) 167, 199
BmeRI GACNNNNNGTC 1 cut(s) 551
BmgBI CACGTC 1 cut(s) 277
BsaHI GRCGYC 2 cut(s) 130, 204
BsaJI CCNNGG 1 cut(s) 195
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 310
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 227, 310
BseNI ACTGG 1 cut(s) 368
BseRI GAGGAG 3 cut(s) 175, 178, 181
BseX3I CGGCCG 1 cut(s) 198
Bsh1236I CGCG 2 cut(s) 197, 218
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 41, 200
BsiEI CGRYCG 1 cut(s) 201
BsiSI CCGG 2 cut(s) 201, 339
BslI CCNNNNNNNGG 2 cut(s) 227, 310
BsnI GGCC 2 cut(s) 41, 200
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 1 cut(s) 451
BspACI CCGC 5 cut(s) 148, 166, 195, 197, 216
BspANI GGCC 2 cut(s) 41, 200
BspFNI CGCG 2 cut(s) 197, 218
BspHI TCATGA 1 cut(s) 252
BspQI GCTCTTC 1 cut(s) 175
BsrBI CCGCTC 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 368
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 1 cut(s) 451
BssNI GRCGYC 2 cut(s) 130, 204
Bst4CI ACNGT 2 cut(s) 241, 298
Bst6I CTCTTC 2 cut(s) 175, 323
BstACI GRCGYC 2 cut(s) 130, 204
BstC8I GCNNGC 2 cut(s) 107, 202
BstDSI CCRYGG 1 cut(s) 195
BstFNI CGCG 2 cut(s) 197, 218
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 1 cut(s) 454
BstMBI GATC 1 cut(s) 451
BstMCI CGRYCG 1 cut(s) 201
BstUI CGCG 2 cut(s) 197, 218
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BstZI CGGCCG 1 cut(s) 198
BsuRI GGCC 2 cut(s) 41, 200
BtgI CCRYGG 1 cut(s) 195
BtgZI GCGATG 1 cut(s) 146
BtrI CACGTC 1 cut(s) 277
Cac8I GCNNGC 2 cut(s) 107, 202
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 200
Cfr42I CCGCGG 1 cut(s) 198
CseI GACGC 1 cut(s) 193
CviAII CATG 2 cut(s) 253, 556
CviJI RGCY 5 cut(s) 9, 41, 200, 286, 370
CviKI_1 RGCY 5 cut(s) 9, 41, 200, 286, 370
DpnI GATC 1 cut(s) 453
DpnII GATC 1 cut(s) 451
DriI GACNNNNNGTC 1 cut(s) 551
EaeI YGGCCR 1 cut(s) 198
EagI CGGCCG 1 cut(s) 198
Eam1104I CTCTTC 2 cut(s) 175, 323
Eam1105I GACNNNNNGTC 1 cut(s) 551
EarI CTCTTC 2 cut(s) 175, 323
EciI GGCGGA 1 cut(s) 137
EclXI CGGCCG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 11
Eco52I CGGCCG 1 cut(s) 198
EcoRI GAATTC 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 2 cut(s) 256, 559
FatI CATG 2 cut(s) 252, 555
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 166, 198
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 2 cut(s) 166, 198
GlaI GCGC 1 cut(s) 219
GluI GCNGC 2 cut(s) 166, 198
HaeIII GGCC 2 cut(s) 41, 200
HapII CCGG 2 cut(s) 201, 339
HgaI GACGC 1 cut(s) 193
HhaI GCGC 1 cut(s) 220
Hin1I GRCGYC 2 cut(s) 130, 204
Hin1II CATG 2 cut(s) 256, 559
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 2 cut(s) 142, 410
HpaII CCGG 2 cut(s) 201, 339
HphI GGTGA 2 cut(s) 63, 70
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 2 cut(s) 388, 456
Hpy188III TCNNGA 4 cut(s) 139, 208, 253, 420
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 4 cut(s) 132, 135, 281, 302
HpyAV CCTTC 3 cut(s) 69, 91, 98
HpyCH4III ACNGT 2 cut(s) 241, 298
HpyCH4IV ACGT 2 cut(s) 130, 276
HpyCH4V TGCA 1 cut(s) 448
HpySE526I ACGT 2 cut(s) 130, 276
Hsp92I GRCGYC 2 cut(s) 130, 204
Hsp92II CATG 2 cut(s) 256, 559
HspAI GCGC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
KspI CCGCGG 1 cut(s) 198
Kzo9I GATC 1 cut(s) 451
LguI GCTCTTC 1 cut(s) 175
LpnPI CCDG 9 cut(s) 23, 152, 214, 305, 349, 352, 356, 360, 405
MaeII ACGT 2 cut(s) 130, 276
MaeIII GTNAC 1 cut(s) 205
MalI GATC 1 cut(s) 453
MbiI CCGCTC 1 cut(s) 168
MboI GATC 1 cut(s) 451
MboII GAAGA 3 cut(s) 54, 162, 340
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 6 cut(s) 174, 211, 333, 457, 548, 564
MlyI GAGTC 2 cut(s) 136, 404
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 3 cut(s) 282, 519, 542
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 2 cut(s) 201, 339
MvnI CGCG 2 cut(s) 197, 218
NaeI GCCGGC 1 cut(s) 202
NdeII GATC 1 cut(s) 451
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 2 cut(s) 256, 559
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 205
PagI TCATGA 1 cut(s) 252
PciSI GCTCTTC 1 cut(s) 175
PdiI GCCGGC 1 cut(s) 202
PflMI CCANNNNNTGG 1 cut(s) 310
PkrI GCNGC 2 cut(s) 167, 199
PleI GAGTC 2 cut(s) 136, 404
PpsI GAGTC 2 cut(s) 136, 404
PsuI RGATCY 1 cut(s) 451
SacII CCGCGG 1 cut(s) 198
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 175
SaqAI TTAA 3 cut(s) 282, 519, 542
SatI GCNGC 2 cut(s) 166, 198
Sau3AI GATC 1 cut(s) 451
SchI GAGTC 2 cut(s) 136, 404
SduI GDGCHC 1 cut(s) 11
SetI ASST 8 cut(s) 53, 76, 83, 118, 133, 279, 372, 436
Sfr303I CCGCGG 1 cut(s) 198
SgrBI CCGCGG 1 cut(s) 198
Sse9I AATT 6 cut(s) 174, 211, 333, 457, 548, 564
SsiI CCGC 5 cut(s) 148, 166, 195, 197, 216
TaaI ACNGT 2 cut(s) 241, 298
TaiI ACGT 2 cut(s) 133, 279
TaqI TCGA 1 cut(s) 300
TasI AATT 6 cut(s) 174, 211, 333, 457, 548, 564
TauI GCSGC 2 cut(s) 168, 200
Tru1I TTAA 3 cut(s) 282, 519, 542
Tru9I TTAA 3 cut(s) 282, 519, 542
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 310
XapI RAATTY 3 cut(s) 211, 457, 564
XmiI GTMKAC 1 cut(s) 300
ZraI GACGTC 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.