Rw6G007430

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013909
Physical Location & Seq
Reverse (-)
12223539 .. 12229319
5781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G007430.1

Sequence Viewer

Length: 726 bp
ATGATGGGCTCAAACTCAATCCAAATCCCAATCGCTCTGGCCTATCACACCTCCCCTCCTTCTTCTCTTTCACCTCTCACCTTCTCCCTTCCTCGCTCGCCTTTACCTCCCTCTCTACGACGTCGTTTCCAGACTCTCCGCCTCGCAATCCATCGCCGCTCTTCAATTCTCGCCTCCTCCTCCTCCGCGGCCGGCGTCACCAATTCCGCGCCGAGAAATGGAAACTACACCGTCAGCGAATTCATGACCACCAAAGAGCATTTACACGTCGTTAAACCCTCCACAACCGTCGACCAAGCATTGGATTTTCTGGTGGAGAAGAGAATTACCGGATTTCCTGTGATCGATGATGACTGGAAGCTGGTTGGTGTTGTTTCGGATTATGACTTGTTAGCATTGGACTCCATATCAGGAAATATAGGAGGTGGTAGTCAACATGACACAAACTTATTTCCGGATGTGGACAGTTCTTGGAAGACATTCAATGAAATACAGAAATTGCTTAGCAAAACGAATGGAAAAGTTGTTGGCGACTTGATGACACCTGCTCCACTTGTTGTTCGTGAAACCACCAACCTAGAAGATGCTGCTAGGTTGTTGCTTGCGACAAAATATCGCCGACTGCCAGTTGTAGATAGTGATGGCAAGCTGGTTGGGATCATTACAAGGGGAAATGTTGTTAAAGCTGCCCTTCAGATAAAACGTGGTAGTCAAAAATTAGCTTAG

Protein Analysis

241

Amino Acids

26.19

Weight (kDa)

9.73

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBS PF00571 78 - 132 9e-13 CBS domain
CBS PF00571 176 - 230 2.2e-17 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013909)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34120
fragaria_vesca FvH4_2g05710 FvH4_2g05710
malus_domestica MD05G1085200.v1.1
prunus_persica Prupe.8G129500_v2.0.a1
pyrus_communis pycom05g08450 pycom1609g00040
rosa_chinensis RchiOBHm_Chr6g0256581
rosa_laevigata RLG00000014753
rosa_multiflora Rmu_sc0003012.1_g000012
rosa_roxburghii Rroxscaffold_7G00209210
rosa_rugosa Rorug05G0564700
rosa_samantha Rh6AG082500 Rh6BG075200 Rh6CG071800 Rh6DG069000
rosa_wichuraiana Rw6G007430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 553
AatII GACGTC 1 cut(s) 124
Acc36I ACCTGC 1 cut(s) 553
AccB7I CCANNNNNTGG 1 cut(s) 301
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 291
AccII CGCG 2 cut(s) 188, 209
AccIII TCCGGA 1 cut(s) 454
AciI CCGC 5 cut(s) 139, 157, 186, 188, 207
AclWI GGATC 1 cut(s) 665
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 677
AcyI GRCGYC 2 cut(s) 121, 195
AfiI CCNNNNNNNGG 2 cut(s) 218, 301
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 2 cut(s) 165, 484
AjiI CACGTC 1 cut(s) 268
AluBI AGCT 4 cut(s) 361, 649, 686, 722
AluI AGCT 4 cut(s) 361, 649, 686, 722
AlwI GGATC 1 cut(s) 665
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 2 cut(s) 39, 189
ApeKI GCWGC 2 cut(s) 587, 686
ApoI RAATTY 1 cut(s) 239
Asp700I GAANNNNTTC 1 cut(s) 479
AspLEI GCGC 1 cut(s) 211
AsuHPI GGTGA 3 cut(s) 63, 70, 190
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 574, 673
BccI CCATC 2 cut(s) 159, 635
BfaI CTAG 2 cut(s) 578, 591
BfuAI ACCTGC 1 cut(s) 553
BisI GCNGC 4 cut(s) 157, 189, 588, 687
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 4 cut(s) 158, 190, 589, 688
BmgBI CACGTC 1 cut(s) 268
BmsI GCATC 1 cut(s) 574
BpiI GAAGAC 1 cut(s) 482
Bpu1102I GCTNAGC 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 2 cut(s) 121, 195
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 2 cut(s) 329, 454
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 532, 562
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 301
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 359, 626
BseAI TCCGGA 1 cut(s) 454
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 2 cut(s) 218, 301
BseNI ACTGG 2 cut(s) 359, 626
BseRI GAGGAG 3 cut(s) 166, 169, 172
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 2 cut(s) 574, 673
Bsh1236I CGCG 2 cut(s) 188, 209
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 2 cut(s) 41, 191
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 1 cut(s) 192
BsiSI CCGG 3 cut(s) 192, 330, 455
BslI CCNNNNNNNGG 2 cut(s) 218, 301
BsnI GGCC 2 cut(s) 41, 191
Bsp1286I GDGCHC 1 cut(s) 11
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 2 cut(s) 342, 657
Bsp1720I GCTNAGC 1 cut(s) 503
BspACI CCGC 5 cut(s) 139, 157, 186, 188, 207
BspANI GGCC 2 cut(s) 41, 191
BspDI ATCGAT 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 2 cut(s) 188, 209
BspHI TCATGA 1 cut(s) 243
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 1 cut(s) 665
BspQI GCTCTTC 1 cut(s) 166
BsrBI CCGCTC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 2 cut(s) 359, 626
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 2 cut(s) 342, 657
BssNI GRCGYC 2 cut(s) 121, 195
Bst4CI ACNGT 3 cut(s) 232, 289, 467
Bst6I CTCTTC 2 cut(s) 166, 314
BstACI GRCGYC 2 cut(s) 121, 195
BstC8I GCNNGC 4 cut(s) 98, 193, 603, 647
BstDEI CTNAG 2 cut(s) 503, 723
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 463
BstFNI CGCG 2 cut(s) 188, 209
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 2 cut(s) 345, 660
BstMBI GATC 2 cut(s) 342, 657
BstMCI CGRYCG 1 cut(s) 192
BstUI CGCG 2 cut(s) 188, 209
BstV1I GCAGC 2 cut(s) 574, 673
BstV2I GAAGAC 1 cut(s) 482
BstZI CGGCCG 1 cut(s) 189
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 2 cut(s) 41, 191
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 186
BtgZI GCGATG 1 cut(s) 137
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 1 cut(s) 463
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 4 cut(s) 98, 193, 603, 647
CciI TCATGA 1 cut(s) 243
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 191
Cfr42I CCGCGG 1 cut(s) 189
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 1 cut(s) 184
CviAII CATG 2 cut(s) 244, 437
CviJI RGCY 7 cut(s) 9, 41, 191, 361, 649, 686, 722
CviKI_1 RGCY 7 cut(s) 9, 41, 191, 361, 649, 686, 722
DdeI CTNAG 2 cut(s) 503, 723
DpnI GATC 2 cut(s) 344, 659
DpnII GATC 2 cut(s) 342, 657
EaeI YGGCCR 1 cut(s) 189
EagI CGGCCG 1 cut(s) 189
Eam1104I CTCTTC 2 cut(s) 166, 314
EarI CTCTTC 2 cut(s) 166, 314
EciI GGCGGA 1 cut(s) 128
EclXI CGGCCG 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 11
Eco52I CGGCCG 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 2 cut(s) 247, 440
FaiI YATR 5 cut(s) 245, 384, 407, 419, 438
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FatI CATG 2 cut(s) 243, 436
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 4 cut(s) 157, 189, 588, 687
FokI GGATG 1 cut(s) 470
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 4 cut(s) 157, 189, 588, 687
FspBI CTAG 2 cut(s) 578, 591
GlaI GCGC 1 cut(s) 210
GluI GCNGC 4 cut(s) 157, 189, 588, 687
HaeIII GGCC 2 cut(s) 41, 191
HapII CCGG 3 cut(s) 192, 330, 455
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 211
Hin1I GRCGYC 2 cut(s) 121, 195
Hin1II CATG 2 cut(s) 247, 440
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HincII GTYRAC 2 cut(s) 292, 434
HindII GTYRAC 2 cut(s) 292, 434
HinfI GANTC 2 cut(s) 133, 401
HpaII CCGG 3 cut(s) 192, 330, 455
HphI GGTGA 3 cut(s) 63, 70, 190
Hpy166II GTNNAC 3 cut(s) 292, 434, 463
Hpy188I TCNGA 2 cut(s) 379, 696
Hpy188III TCNNGA 5 cut(s) 130, 244, 411, 455, 563
Hpy8I GTNNAC 3 cut(s) 292, 434, 463
Hpy99I CGWCG 4 cut(s) 123, 126, 272, 293
HpyAV CCTTC 4 cut(s) 69, 91, 98, 701
HpyCH4III ACNGT 3 cut(s) 232, 289, 467
HpyCH4IV ACGT 3 cut(s) 121, 267, 703
HpyF3I CTNAG 2 cut(s) 503, 723
HpySE526I ACGT 3 cut(s) 121, 267, 703
Hsp92I GRCGYC 2 cut(s) 121, 195
Hsp92II CATG 2 cut(s) 247, 440
HspAI GCGC 1 cut(s) 209
Kpn2I TCCGGA 1 cut(s) 454
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
KspI CCGCGG 1 cut(s) 189
Kzo9I GATC 2 cut(s) 342, 657
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 2 cut(s) 574, 673
LweI GCATC 1 cut(s) 574
MaeI CTAG 2 cut(s) 578, 591
MaeII ACGT 3 cut(s) 121, 267, 703
MaeIII GTNAC 1 cut(s) 196
MalI GATC 2 cut(s) 344, 659
MbiI CCGCTC 1 cut(s) 159
MboI GATC 2 cut(s) 342, 657
MboII GAAGA 5 cut(s) 54, 153, 331, 487, 593
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 6 cut(s) 165, 202, 239, 324, 497, 716
MlyI GAGTC 2 cut(s) 127, 395
MroI TCCGGA 1 cut(s) 454
MroNI GCCGGC 1 cut(s) 191
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 2 cut(s) 273, 681
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 3 cut(s) 192, 330, 455
MvnI CGCG 2 cut(s) 188, 209
NaeI GCCGGC 1 cut(s) 193
NdeII GATC 2 cut(s) 342, 657
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 2 cut(s) 247, 440
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 196
PagI TCATGA 1 cut(s) 243
PaqCI CACCTGC 1 cut(s) 553
PciSI GCTCTTC 1 cut(s) 166
PdiI GCCGGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 4 cut(s) 158, 190, 589, 688
PleI GAGTC 2 cut(s) 127, 395
PpsI GAGTC 2 cut(s) 127, 395
SacII CCGCGG 1 cut(s) 189
SalI GTCGAC 1 cut(s) 290
SapI GCTCTTC 1 cut(s) 166
SaqAI TTAA 2 cut(s) 273, 681
SatI GCNGC 4 cut(s) 157, 189, 588, 687
Sau3AI GATC 2 cut(s) 342, 657
SchI GAGTC 2 cut(s) 127, 395
SduI GDGCHC 1 cut(s) 11
SfaNI GCATC 1 cut(s) 574
Sfr303I CCGCGG 1 cut(s) 189
SgrBI CCGCGG 1 cut(s) 189
Sse9I AATT 6 cut(s) 165, 202, 239, 324, 497, 716
SsiI CCGC 5 cut(s) 139, 157, 186, 188, 207
SspMI CTAG 2 cut(s) 578, 591
TaaI ACNGT 3 cut(s) 232, 289, 467
TaiI ACGT 3 cut(s) 124, 270, 706
TaqI TCGA 2 cut(s) 291, 345
TasI AATT 6 cut(s) 165, 202, 239, 324, 497, 716
TauI GCSGC 2 cut(s) 159, 191
Tru1I TTAA 2 cut(s) 273, 681
Tru9I TTAA 2 cut(s) 273, 681
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 587, 686
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 232, 501
Van91I CCANNNNNTGG 1 cut(s) 301
XapI RAATTY 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 2 cut(s) 578, 591
ZraI GACGTC 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.