Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0007882

Physical Location & Seq

Genomic Coordinates Forward (+)

61128148 .. 61134284

Gene Structure 6137 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG432200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGCCCCGCCTTTCGTGTTCGAACCCCCGAGCGATGAAGAATATTCCGAAGCAGAGGAAGAAGACTACTCAGACGAAGAAGAAGAACAACAAGAGGAACAAGAAGAAGAGGCGTCTGTAAAACCCTCGCGTGCTCAATCCCCATGGGACTTCGCTTCCTACTCCGAAACCGTCGCCGAAGAACACGCCCGCCGGAGCACCACCTCCGTCGACGACAAAATCTCCAAAGCCCGCCAGCAACTCTCCGTCCCGCTCCCCGACCCGGACGACGCTTCTAGCTCCGGATCCGAATCCGATAGACAAGAAGATTATAAACCAGAAGATGAGGAAGACGATGTAGGTACAAATGCGGCTGAGAACAAGCCTTTCTTTGCTTCATCTGACGGCACGTCGTTCCATGCGAATTCGTTCCTGGAGCTCAATTTGTCGAGACCCCTGCTTCGGGCTTGCGAGAAATTGGGCTATGTTAAGCCCACGCCGATTCAGGCAGCGTGCATTCCACTGGCCTTGTCTGGGAGGGATATATGCGGAAGTGCCATTACTGGTTCCGGGAAGACGGCTGCTTTTGCGTTACCTACACTGGAGAGATTATTGTTTCGCCCCAAGCGGGTGCCTGCCATAAGGGTCCTTGTTCTGACTCCTGCCAGAGAACTGGCGGTTCAGGTTCATAGTATGATTGAGAAACTTGCTCAATTTACTGATATCAGATGTTGCCTGGTTGTTGGAGGCCTTTCACTAAAGGTTCAAGAAGCAGCCTTGAGATCCATGCCGGATATCGTCGTGGCTACCCCGGGACGCATTATAGATCATTTACGAAATTCTATGTCTGTGGATTTGGATGATCTTGCTGTTCTAATTCTTGATGAGGCAGATCGCCTGCTGGAAGTTGGGTTTAGTGCTGAAATTCGTGAGCTGCTTCGAGTATGCCCCAAAAGGAGACAAACCATGCTATTTTCAGCTACGATGACTGAAGAAGTTGATGAACTTGTCCAGCTTTCTCTCACCAAACCACTACGCCTTTCAGCTGACCCATCCGCGAAACGGCCAGTGACACTAACTGAGGAGGTGGTTCGGATACGACGTATGCGTGAACTAAACCAGGAAGCAGTTCTACTTGCATTGTGCACAAAGACATTCAGAGAAAGAGTGATCATATTCAGTGGGACAAAACAGGCTGCACATAGGCTGAAGATCTTATTTGGGTTAGCTGGCTTAAAAGCCGCTGAGCTTCATGGCAACCTTACTCAAGTGCAGCGCCTAGATGCATTGGAACTTTTCAGGAGGGAAAAGGTTGACTTTTTGATTGCAACAGATGTCGCTGCTCGTGGTCTTGACATAATTGGTGTTGAAACAGTTATAAATTATGCATGTCCACGTGATCTTACAAGCTATGTTCATCGAGTGGGTCGTACAGCAAGAGCTGGGAAAGAGGGATGCGCTGTTACTTTCGTGACAGATACTGACCGATCTCTGTTAAAAGCTATTGCAAAAAGAGCTGGTTCAAAGCTGAGGAGTCGAATTGTTGCAGAGCAGTCAATTACTAAGTGGTCTCAGATAATTGAGCAAATGGAAGATCAAGTGGCTACAATTCTTCGAGAAGAGAGCGAAGAGACAGCGCTAAGAAGAGCTGAAATGGAAGCAACAAAGGTAGAGAATTTGATTGCACACAGAGATGAAATTTATTCACGCCCCAAAAGGACCTGGATTGTGACTGAAAAGGAGAAGAAGGAGGTAGTGAAGGCATCTAAGGCATCCGGTGAAAGAGAAAAACATTCTGGAAACGAGGTGATCAGTGCTGAACAAGCGGAAGAACTGAAAATGAAAGAAAAGAGGAAACGAGAGCGTGAGAAAAATTTGCCGCGTAAGAAGCGCCGAAATTTGGAAGCAGCTAGAGAAATGTTGGAGGAAGAAAATCAAAATCGAAAGTCAGAAGGTAGTGGAACAACCAAGGACAAGACAGGAGTGAAACTTGTTGACTTGGGTTACCGACGGGCAAAAGCTGTGAAGGCAGCAAATAAGGCAATGGATACTGGCAAGATTGTGAAGAAGGATAGCAAGAAATCTAATCGTCTTCCGCAAAGAACCCAGTCAAGGACAGATGAGATGCAGGACCTGTTCCAGAGTGATATGAGTCAAGGGAAAAAGGATCGAAAAAGCGGTGGCATGGGAAAGAAAAAGTCCAAGAATTCATTTAAAAGCAAGTCGAGGTACAAACGAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

740

Amino Acids

83.16

Weight (kDa)

8.67

Isoelectric Point (pI)

51.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	159 - 329	2.1e-45	DEAD/DEAH box helicase
Helicase_C	PF00271	370 - 475	8.3e-28	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007882)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16630
fragaria_vesca	FvH4_2g38260 FvH4_2g38260 FvH4_2g38260 FvH4_2g38260 FvH4_2g38260 FvH4_2g38260
malus_domestica	MD01G1061100.v1.1 MD15G1442700.v1.1
prunus_persica	Prupe.1G363600_v2.0.a1 Prupe.3G157600_v2.0.a1
pyrus_communis	pycom15g39060
rosa_chinensis	RchiOBHm_Chr6g0303111
rosa_laevigata	RLG00000011107
rosa_multiflora	Rmu_co8350977.1_g000001 Rmu_sc0003721.1_g000002 Rmu_sc0008911.1_g000005
rosa_roxburghii	Rroxscaffold_7G00165010
rosa_rugosa	Rorug06G0319200
rosa_samantha	Rh6AG433100 Rh6BG469600 Rh6CG445400 Rh6DG432200
rosa_wichuraiana	Rw6G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	312, 1358
AccB1I	GGYRCC	1 cut(s)	610
AccBSI	CCGCTC	1 cut(s)	253
AccI	GTMKAC	1 cut(s)	210
AccII	CGCG	3 cut(s)	130, 1037, 1861
AccIII	TCCGGA	1 cut(s)	281
AclWI	GGATC	4 cut(s)	279, 292, 756, 2154
AcoI	YGGCCR	1 cut(s)	1043
AcsI	RAATTY	8 cut(s)	403, 817, 903, 1654, 1677, 1852, 1876, 2185
AcuI	CTGAAG	2 cut(s)	990, 1208
AcvI	CACGTG	1 cut(s)	1376
AcyI	GRCGYC	1 cut(s)	113
AfaI	GTAC	3 cut(s)	343, 1411, 2210
AfeI	AGCGCT	1 cut(s)	1617
AfiI	CCNNNNNNNGG	8 cut(s)	262, 441, 442, 513, 607, 1041, 1879, 2091
AgsI	TTSAA	3 cut(s)	746, 1349, 1503
AhdI	GACNNNNNGTC	1 cut(s)	388
AjiI	CACGTC	1 cut(s)	390
AjnI	CCWGG	4 cut(s)	411, 714, 1098, 1700
Alw21I	GWGCWC	4 cut(s)	136, 200, 420, 1127
Alw26I	GTCTC	4 cut(s)	424, 931, 1554, 1604
Alw44I	GTGCAC	1 cut(s)	1123
AlwI	GGATC	4 cut(s)	279, 292, 756, 2154
AlwNI	CAGNNNCTG	2 cut(s)	1460, 2113
Ama87I	CYCGRG	2 cut(s)	28, 790
Aor13HI	TCCGGA	1 cut(s)	281
Aor51HI	AGCGCT	1 cut(s)	1617
AoxI	GGCC	4 cut(s)	3, 504, 727, 1043
ApaLI	GTGCAC	1 cut(s)	1123
ApeKI	GCWGC	9 cut(s)	488, 560, 752, 913, 1175, 1252, 1319, 1886, 2009
ApoI	RAATTY	8 cut(s)	403, 817, 903, 1654, 1677, 1852, 1876, 2185
ArsI	GACNNNNNNTTYG	2 cut(s)	423, 455
Asp700I	GAANNNNTTC	2 cut(s)	407, 1107
AspLEI	GCGC	4 cut(s)	1257, 1439, 1618, 1872
AspS9I	GGNCC	4 cut(s)	4, 625, 1698, 2110
AsuC2I	CCSGG	4 cut(s)	263, 550, 791, 792
AsuHPI	GGTGA	3 cut(s)	994, 1769, 1798
AsuII	TTCGAA	1 cut(s)	21
AvaI	CYCGRG	2 cut(s)	28, 790
AvaII	GGWCC	3 cut(s)	625, 1698, 2110
BaeGI	GKGCMC	1 cut(s)	1127
BamHI	GGATCC	1 cut(s)	284
BanI	GGYRCC	1 cut(s)	610
BanII	GRGCYC	1 cut(s)	420
BarI	GAAGNNNNNNTAC	4 cut(s)	523, 555, 1095, 1127
BauI	CACGAG	1 cut(s)	1323
BbrPI	CACGTG	1 cut(s)	1376
BbsI	GAAGAC	4 cut(s)	69, 336, 560, 2063
Bbv12I	GWGCWC	4 cut(s)	136, 200, 420, 1127
BbvCI	CCTCAGC	1 cut(s)	1508
BbvI	GCAGC	9 cut(s)	500, 547, 764, 900, 1162, 1264, 1306, 1898, 2021
BccI	CCATC	1 cut(s)	1039
BceAI	ACGGC	3 cut(s)	400, 573, 1058
BcgI	CGANNNNNNTGC	2 cut(s)	418, 452
BciT130I	CCWGG	4 cut(s)	413, 716, 1100, 1702
BciVI	GTATCC	2 cut(s)	1068, 2020
BclI	TGATCA	2 cut(s)	1149, 1788
BcnI	CCSGG	4 cut(s)	263, 550, 791, 792
BcoDI	GTCTC	4 cut(s)	424, 931, 1554, 1604
BfaI	CTAG	3 cut(s)	276, 1259, 1890
BfoI	RGCGCY	3 cut(s)	1258, 1619, 1873
BfuI	GTATCC	2 cut(s)	1068, 2020
BglII	AGATCT	1 cut(s)	1191
BlpI	GCTNAGC	1 cut(s)	1224
Bme1390I	CCNGG	8 cut(s)	263, 413, 550, 716, 791, 792, 1100, 1702
Bme18I	GGWCC	3 cut(s)	625, 1698, 2110
BmeRI	GACNNNNNGTC	1 cut(s)	388
BmeT110I	CYCGRG	2 cut(s)	28, 790
BmgBI	CACGTC	1 cut(s)	390
BmgT120I	GGNCC	4 cut(s)	4, 625, 1698, 2110
BmiI	GGNNCC	5 cut(s)	6, 286, 547, 612, 626
BmrFI	CCNGG	8 cut(s)	263, 413, 550, 716, 791, 792, 1100, 1702
BmrI	ACTGGG	1 cut(s)	2080
BmsI	GCATC	5 cut(s)	1252, 1424, 1751, 1760, 2094
BmuI	ACTGGG	1 cut(s)	2080
BpiI	GAAGAC	4 cut(s)	69, 336, 560, 2063
BpmI	CTGGAG	2 cut(s)	434, 602
Bpu10I	CCTNAGC	1 cut(s)	1508
Bpu1102I	GCTNAGC	1 cut(s)	1224
Bpu14I	TTCGAA	1 cut(s)	21
BpuEI	CTTGAG	2 cut(s)	778, 1230
BpuMI	CCSGG	4 cut(s)	263, 550, 791, 792
BsaAI	YACGTR	1 cut(s)	1376
BsaBI	GATNNNNATC	1 cut(s)	289
BsaHI	GRCGYC	1 cut(s)	113
BsaI	GGTCTC	2 cut(s)	424, 1554
BsaJI	CCNNGG	4 cut(s)	143, 789, 790, 1947
BsaWI	WCCGGW	2 cut(s)	281, 1754
BsaXI	ACNNNNNCTCC	2 cut(s)	206, 236
Bsc4I	CCNNNNNNNGG	8 cut(s)	262, 441, 442, 513, 607, 1041, 1879, 2091
Bse1I	ACTGG	7 cut(s)	507, 547, 585, 657, 1046, 2035, 2086
Bse3DI	GCAATG	1 cut(s)	2028
Bse8I	GATNNNNATC	1 cut(s)	289
BseAI	TCCGGA	1 cut(s)	281
BseBI	CCWGG	4 cut(s)	413, 716, 1100, 1702
BseDI	CCNNGG	4 cut(s)	143, 789, 790, 1947
BseGI	GGATG	4 cut(s)	844, 1031, 1439, 1751
BseJI	GATNNNNATC	1 cut(s)	289
BseLI	CCNNNNNNNGG	8 cut(s)	262, 441, 442, 513, 607, 1041, 1879, 2091
BseMI	GCAATG	1 cut(s)	2028
BseMII	CTCAG	6 cut(s)	84, 345, 1050, 1215, 1499, 1565
BseNI	ACTGG	7 cut(s)	507, 547, 585, 657, 1046, 2035, 2086
BseRI	GAGGAG	2 cut(s)	1076, 1525
BseSI	GKGCMC	1 cut(s)	1127
BseXI	GCAGC	9 cut(s)	500, 547, 764, 900, 1162, 1264, 1306, 1898, 2021
BseYI	CCCAGC	1 cut(s)	1421
BsgI	GTGCAG	2 cut(s)	1161, 1271
Bsh1236I	CGCG	3 cut(s)	130, 1037, 1861
BshFI	GGCC	4 cut(s)	5, 506, 729, 1045
BshNI	GGYRCC	1 cut(s)	610
BsiHKAI	GWGCWC	4 cut(s)	136, 200, 420, 1127
BsiHKCI	CYCGRG	2 cut(s)	28, 790
BsiSI	CCGG	7 cut(s)	193, 263, 282, 549, 770, 791, 1755
BslFI	GGGAC	4 cut(s)	161, 233, 807, 1177
BslI	CCNNNNNNNGG	8 cut(s)	262, 441, 442, 513, 607, 1041, 1879, 2091
BsmAI	GTCTC	4 cut(s)	424, 931, 1554, 1604
BsmFI	GGGAC	4 cut(s)	161, 233, 807, 1177
BsmI	GAATGC	1 cut(s)	495
BsnI	GGCC	4 cut(s)	5, 506, 729, 1045
Bso31I	GGTCTC	2 cut(s)	424, 1554
BsoBI	CYCGRG	2 cut(s)	28, 790
Bsp119I	TTCGAA	1 cut(s)	21
Bsp1286I	GDGCHC	4 cut(s)	136, 200, 420, 1127
Bsp13I	TCCGGA	1 cut(s)	281
Bsp1720I	GCTNAGC	1 cut(s)	1224
Bsp19I	CCATGG	1 cut(s)	143
BspANI	GGCC	4 cut(s)	5, 506, 729, 1045
BspCNI	CTCAG	6 cut(s)	83, 346, 1051, 1216, 1500, 1564
BspEI	TCCGGA	1 cut(s)	281
BspFNI	CGCG	3 cut(s)	130, 1037, 1861
BspLI	GGNNCC	5 cut(s)	6, 286, 547, 612, 626
BspPI	GGATC	4 cut(s)	279, 292, 756, 2154
BspQI	GCTCTTC	1 cut(s)	1618
BspT104I	TTCGAA	1 cut(s)	21
BspT107I	GGYRCC	1 cut(s)	610
BspTNI	GGTCTC	2 cut(s)	424, 1554
BsrBI	CCGCTC	1 cut(s)	253
BsrDI	GCAATG	1 cut(s)	2028
BsrI	ACTGG	7 cut(s)	507, 547, 585, 657, 1046, 2035, 2086
BssECI	CCNNGG	4 cut(s)	143, 789, 790, 1947
BssNI	GRCGYC	1 cut(s)	113
BssSI	CACGAG	1 cut(s)	1323
BssT1I	CCWWGG	2 cut(s)	143, 1947
Bst2BI	CACGAG	1 cut(s)	1323
Bst2UI	CCWGG	4 cut(s)	413, 716, 1100, 1702
Bst4CI	ACNGT	2 cut(s)	172, 1354
Bst6I	CTCTTC	4 cut(s)	102, 1593, 1602, 1618
BstACI	GRCGYC	1 cut(s)	113
BstBAI	YACGTR	1 cut(s)	1376
BstBI	TTCGAA	1 cut(s)	21
BstC8I	GCNNGC	9 cut(s)	132, 190, 232, 236, 448, 493, 615, 878, 1210
BstDEI	CTNAG	9 cut(s)	70, 354, 1059, 1224, 1508, 1542, 1551, 1619, 1746
BstDSI	CCRYGG	1 cut(s)	143
BstEII	GGTNACC	1 cut(s)	1982
BstF5I	GGATG	4 cut(s)	844, 1031, 1439, 1751
BstFNI	CGCG	3 cut(s)	130, 1037, 1861
BstH2I	RGCGCY	3 cut(s)	1258, 1619, 1873
BstHHI	GCGC	4 cut(s)	1257, 1439, 1618, 1872
BstMAI	GTCTC	4 cut(s)	424, 931, 1554, 1604
BstMWI	GCNNNNNNNGC	7 cut(s)	566, 1493, 1748, 1802, 1867, 2006, 2018
BstNI	CCWGG	4 cut(s)	413, 716, 1100, 1702
BstNSI	RCATGY	1 cut(s)	1371
BstPI	GGTNACC	1 cut(s)	1982
BstSCI	CCNGG	8 cut(s)	261, 411, 548, 714, 789, 790, 1098, 1700
BstSLI	GKGCMC	1 cut(s)	1127
BstUI	CGCG	3 cut(s)	130, 1037, 1861
BstV1I	GCAGC	9 cut(s)	500, 547, 764, 900, 1162, 1264, 1306, 1898, 2021
BstV2I	GAAGAC	4 cut(s)	69, 336, 560, 2063
BstX2I	RGATCY	3 cut(s)	284, 761, 1191
BstXI	CCANNNNNNTGG	1 cut(s)	652
BstYI	RGATCY	3 cut(s)	284, 761, 1191
BsuI	GTATCC	2 cut(s)	1068, 2020
BsuRI	GGCC	4 cut(s)	5, 506, 729, 1045
BtgI	CCRYGG	1 cut(s)	143
BtgZI	GCGATG	1 cut(s)	48
BtrI	CACGTC	1 cut(s)	390
BtsCI	GGATG	4 cut(s)	844, 1031, 1439, 1751
BtsIMutI	CAGTG	5 cut(s)	500, 578, 1053, 1165, 1798
Cac8I	GCNNGC	9 cut(s)	132, 190, 232, 236, 448, 493, 615, 878, 1210
CaiI	CAGNNNCTG	2 cut(s)	1460, 2113
CfoI	GCGC	4 cut(s)	1257, 1439, 1618, 1872
Cfr13I	GGNCC	4 cut(s)	4, 625, 1698, 2110
Cfr9I	CCCGGG	1 cut(s)	790
CseI	GACGC	3 cut(s)	102, 278, 804
Csp6I	GTAC	3 cut(s)	342, 1410, 2209
CspCI	CAANNNNNGTGG	2 cut(s)	489, 524
CviAII	CATG	7 cut(s)	144, 398, 766, 946, 1232, 1368, 2164
CviQI	GTAC	3 cut(s)	342, 1410, 2209
DdeI	CTNAG	9 cut(s)	70, 354, 1059, 1224, 1508, 1542, 1551, 1619, 1746
DraI	TTTAAA	1 cut(s)	2194
DriI	GACNNNNNGTC	1 cut(s)	388
EaeI	YGGCCR	1 cut(s)	1043
Eam1104I	CTCTTC	4 cut(s)	102, 1593, 1602, 1618
Eam1105I	GACNNNNNGTC	1 cut(s)	388
EarI	CTCTTC	4 cut(s)	102, 1593, 1602, 1618
Ecl136II	GAGCTC	1 cut(s)	418
Eco130I	CCWWGG	2 cut(s)	143, 1947
Eco147I	AGGCCT	1 cut(s)	729
Eco24I	GRGCYC	1 cut(s)	420
Eco31I	GGTCTC	2 cut(s)	424, 1554
Eco32I	GATATC	2 cut(s)	703, 775
Eco47I	GGWCC	3 cut(s)	625, 1698, 2110
Eco47III	AGCGCT	1 cut(s)	1617
Eco53kI	GAGCTC	1 cut(s)	418
Eco57I	CTGAAG	2 cut(s)	990, 1208
Eco72I	CACGTG	1 cut(s)	1376
Eco88I	CYCGRG	2 cut(s)	28, 790
Eco91I	GGTNACC	1 cut(s)	1982
EcoICRI	GAGCTC	1 cut(s)	418
EcoO109I	RGGNCCY	3 cut(s)	625, 1698, 2110
EcoO65I	GGTNACC	1 cut(s)	1982
EcoRI	GAATTC	2 cut(s)	403, 2185
EcoRII	CCWGG	4 cut(s)	411, 714, 1098, 1700
EcoRV	GATATC	2 cut(s)	703, 775
EcoT14I	CCWWGG	2 cut(s)	143, 1947
EcoT22I	ATGCAT	2 cut(s)	1267, 1369
EcoT38I	GRGCYC	1 cut(s)	420
ErhI	CCWWGG	2 cut(s)	143, 1947
FaeI	CATG	7 cut(s)	147, 401, 769, 949, 1235, 1371, 2167
FalI	AAGNNNNNCTT	4 cut(s)	353, 385, 1280, 1312
FaqI	GGGAC	4 cut(s)	161, 233, 807, 1177
FatI	CATG	7 cut(s)	143, 397, 765, 945, 1231, 1367, 2163
FauI	CCCGC	5 cut(s)	15, 197, 239, 258, 600
FbaI	TGATCA	2 cut(s)	1149, 1788
FblI	GTMKAC	1 cut(s)	210
FokI	GGATG	4 cut(s)	851, 1018, 1446, 1738
FriOI	GRGCYC	1 cut(s)	420
FspBI	CTAG	3 cut(s)	276, 1259, 1890
GlaI	GCGC	4 cut(s)	1256, 1438, 1617, 1871
GsaI	CCCAGC	1 cut(s)	1425
GsuI	CTGGAG	2 cut(s)	434, 602
HaeII	RGCGCY	3 cut(s)	1258, 1619, 1873
HaeIII	GGCC	4 cut(s)	5, 506, 729, 1045
HapII	CCGG	7 cut(s)	193, 263, 282, 549, 770, 791, 1755
HgaI	GACGC	3 cut(s)	102, 278, 804
HhaI	GCGC	4 cut(s)	1257, 1439, 1618, 1872
Hin1I	GRCGYC	1 cut(s)	113
Hin1II	CATG	7 cut(s)	147, 401, 769, 949, 1235, 1371, 2167
Hin6I	GCGC	4 cut(s)	1255, 1437, 1616, 1870
HinP1I	GCGC	4 cut(s)	1255, 1437, 1616, 1870
HincII	GTYRAC	3 cut(s)	211, 1294, 1975
HindII	GTYRAC	3 cut(s)	211, 1294, 1975
HinfI	GANTC	5 cut(s)	290, 481, 637, 1513, 2131
HpaII	CCGG	7 cut(s)	193, 263, 282, 549, 770, 791, 1755
HphI	GGTGA	3 cut(s)	994, 1769, 1798
Hpy166II	GTNNAC	6 cut(s)	211, 1091, 1125, 1294, 1373, 1975
Hpy8I	GTNNAC	6 cut(s)	211, 1091, 1125, 1294, 1373, 1975
Hpy99I	CGWCG	8 cut(s)	176, 212, 215, 272, 394, 782, 1083, 1992
HpyAV	CCTTC	5 cut(s)	1720, 1732, 1925, 1999, 2041
HpyCH4III	ACNGT	2 cut(s)	172, 1354
HpyCH4IV	ACGT	3 cut(s)	389, 1081, 1375
HpyF10VI	GCNNNNNNNGC	7 cut(s)	566, 1493, 1748, 1802, 1867, 2006, 2018
HpyF3I	CTNAG	9 cut(s)	70, 354, 1059, 1224, 1508, 1542, 1551, 1619, 1746
HpySE526I	ACGT	3 cut(s)	389, 1081, 1375
Hsp92I	GRCGYC	1 cut(s)	113
Hsp92II	CATG	7 cut(s)	147, 401, 769, 949, 1235, 1371, 2167
HspAI	GCGC	4 cut(s)	1255, 1437, 1616, 1870
Kpn2I	TCCGGA	1 cut(s)	281
Ksp22I	TGATCA	2 cut(s)	1149, 1788
LguI	GCTCTTC	1 cut(s)	1618
LmnI	GCTCC	4 cut(s)	195, 258, 284, 415
Lsp1109I	GCAGC	9 cut(s)	500, 547, 764, 900, 1162, 1264, 1306, 1898, 2021
LweI	GCATC	5 cut(s)	1252, 1424, 1751, 1760, 2094
MaeI	CTAG	3 cut(s)	276, 1259, 1890
MaeII	ACGT	3 cut(s)	389, 1081, 1375
MaeIII	GTNAC	6 cut(s)	570, 1048, 1441, 1450, 1708, 1982
MbiI	CCGCTC	1 cut(s)	253
MflI	RGATCY	3 cut(s)	284, 761, 1191
MhlI	GDGCHC	4 cut(s)	136, 200, 420, 1127
MlyI	GAGTC	3 cut(s)	631, 1522, 2140
MmeI	TCCRAC	2 cut(s)	703, 1881
Mph1103I	ATGCAT	2 cut(s)	1267, 1369
MroI	TCCGGA	1 cut(s)	281
MroXI	GAANNNNTTC	2 cut(s)	407, 1107
MseI	TTAA	4 cut(s)	468, 1214, 1475, 2193
MslI	CAYNNNNRTG	1 cut(s)	1671
MspA1I	CMGCKG	2 cut(s)	1025, 1223
MspI	CCGG	7 cut(s)	193, 263, 282, 549, 770, 791, 1755
MspR9I	CCNGG	8 cut(s)	263, 413, 550, 716, 791, 792, 1100, 1702
Mva1269I	GAATGC	1 cut(s)	495
MvaI	CCWGG	4 cut(s)	413, 716, 1100, 1702
MvnI	CGCG	3 cut(s)	130, 1037, 1861
MwoI	GCNNNNNNNGC	7 cut(s)	566, 1493, 1748, 1802, 1867, 2006, 2018
NciI	CCSGG	4 cut(s)	263, 550, 791, 792
NcoI	CCATGG	1 cut(s)	143
NlaIII	CATG	7 cut(s)	147, 401, 769, 949, 1235, 1371, 2167
NlaIV	GGNNCC	5 cut(s)	6, 286, 547, 612, 626
NmuCI	GTSAC	3 cut(s)	1048, 1450, 1708
NsiI	ATGCAT	2 cut(s)	1267, 1369
NspI	RCATGY	1 cut(s)	1371
NspV	TTCGAA	1 cut(s)	21
PceI	AGGCCT	1 cut(s)	729
PciSI	GCTCTTC	1 cut(s)	1618
PcsI	WCGNNNNNNNCGW	4 cut(s)	398, 1078, 1084, 1405
PctI	GAATGC	1 cut(s)	495
PdmI	GAANNNNTTC	2 cut(s)	407, 1107
PfeI	GAWTC	2 cut(s)	290, 481
PfoI	TCCNGGA	2 cut(s)	411, 548
PleI	GAGTC	3 cut(s)	631, 1521, 2139
PmaCI	CACGTG	1 cut(s)	1376
PmlI	CACGTG	1 cut(s)	1376
PpsI	GAGTC	3 cut(s)	631, 1521, 2139
Ppu21I	YACGTR	1 cut(s)	1376
PpuMI	RGGWCCY	3 cut(s)	625, 1698, 2110
PsiI	TTATAA	2 cut(s)	312, 1358
Psp124BI	GAGCTC	1 cut(s)	420
Psp5II	RGGWCCY	3 cut(s)	625, 1698, 2110
Psp6I	CCWGG	4 cut(s)	411, 714, 1098, 1700
PspCI	CACGTG	1 cut(s)	1376
PspEI	GGTNACC	1 cut(s)	1982
PspFI	CCCAGC	1 cut(s)	1421
PspGI	CCWGG	4 cut(s)	411, 714, 1098, 1700
PspN4I	GGNNCC	5 cut(s)	6, 286, 547, 612, 626
PspPI	GGNCC	4 cut(s)	4, 625, 1698, 2110
PspPPI	RGGWCCY	3 cut(s)	625, 1698, 2110
PstNI	CAGNNNCTG	2 cut(s)	1460, 2113
PsuI	RGATCY	3 cut(s)	284, 761, 1191
PvuII	CAGCTG	1 cut(s)	1025
RsaI	GTAC	3 cut(s)	343, 1411, 2210
RsaNI	GTAC	3 cut(s)	342, 1410, 2209
RseI	CAYNNNNRTG	1 cut(s)	1671
SacI	GAGCTC	1 cut(s)	420
SalI	GTCGAC	1 cut(s)	209
SapI	GCTCTTC	1 cut(s)	1618
SaqAI	TTAA	4 cut(s)	468, 1214, 1475, 2193
Sau96I	GGNCC	4 cut(s)	4, 625, 1698, 2110
SchI	GAGTC	3 cut(s)	631, 1522, 2140
ScrFI	CCNGG	8 cut(s)	263, 413, 550, 716, 791, 792, 1100, 1702
SduI	GDGCHC	4 cut(s)	136, 200, 420, 1127
SfaNI	GCATC	5 cut(s)	1252, 1424, 1751, 1760, 2094
SfuI	TTCGAA	1 cut(s)	21
SgrDI	CGTCGACG	1 cut(s)	209
SinI	GGWCC	3 cut(s)	625, 1698, 2110
SmaI	CCCGGG	1 cut(s)	792
SmiMI	CAYNNNNRTG	1 cut(s)	1671
SmlI	CTYRAG	2 cut(s)	757, 1245
SmoI	CTYRAG	2 cut(s)	757, 1245
SseBI	AGGCCT	1 cut(s)	729
SspI	AATATT	1 cut(s)	44
SspMI	CTAG	3 cut(s)	276, 1259, 1890
SstI	GAGCTC	1 cut(s)	420
StuI	AGGCCT	1 cut(s)	729
StyD4I	CCNGG	8 cut(s)	261, 411, 548, 714, 789, 790, 1098, 1700
StyI	CCWWGG	2 cut(s)	143, 1947
TaaI	ACNGT	2 cut(s)	172, 1354
TaiI	ACGT	3 cut(s)	392, 1084, 1378
TaqII	GACCGA	1 cut(s)	1479
TauI	GCSGC	3 cut(s)	353, 1223, 1861
TfiI	GAWTC	2 cut(s)	290, 481
Tru1I	TTAA	4 cut(s)	468, 1214, 1475, 2193
Tru9I	TTAA	4 cut(s)	468, 1214, 1475, 2193
TscAI	CASTG	5 cut(s)	507, 585, 1053, 1165, 1798
TseFI	GTSAC	3 cut(s)	1048, 1450, 1708
TseI	GCWGC	9 cut(s)	488, 560, 752, 913, 1175, 1252, 1319, 1886, 2009
Tsp45I	GTSAC	3 cut(s)	1048, 1450, 1708
TspDTI	ATGAA	9 cut(s)	51, 366, 656, 996, 1220, 1385, 1689, 1835, 2178
TspGWI	ACGGA	2 cut(s)	196, 235
TspMI	CCCGGG	1 cut(s)	790
TspRI	CASTG	5 cut(s)	507, 585, 1053, 1165, 1798
VneI	GTGCAC	1 cut(s)	1123
VpaK11BI	GGWCC	3 cut(s)	625, 1698, 2110
XapI	RAATTY	8 cut(s)	403, 817, 903, 1654, 1677, 1852, 1876, 2185
XceI	RCATGY	1 cut(s)	1371
XmaI	CCCGGG	1 cut(s)	790
XmiI	GTMKAC	1 cut(s)	210
XmnI	GAANNNNTTC	2 cut(s)	407, 1107
XspI	CTAG	3 cut(s)	276, 1259, 1890
Zsp2I	ATGCAT	2 cut(s)	1267, 1369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.