Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0007882

Physical Location & Seq

Genomic Coordinates Forward (+)

61827578 .. 61834016

Gene Structure 6439 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G037540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGGCCCCGTCTTTCGTGTTCGAACCCCCGAGCGATGAAGAATATTCCGAAGCAGAGGAAGAAGACTACTCAGACGAAGAAGAAGAAGAAGAACAAGAGGAACAAGAAGCGTCTGTAAAACCCTCGCGTGCTCGCACTTCTCAATCCCCATGGGACTTCGCTTCCTACTCCGAAACCGTCGCCGAAGAACACGCCCGCCGGAGCACCACCTCCGTCGACGACAAAATCTCCAAAGCCCGCCAGCAACTATCCGTCCCGCTCCCCGACCCGGACGACGCTTCTAGCTCCGGATCCGAATCCGATAGACAAGAAGATTATAAACCAGAAGATGAGGAAGACGATGTAGGTGCAAATGCGGCTGAGAATAAGCCTTTCTTTGCTTCATCCGACGGCACGTCGTTTCATGCGAATTCGTTCCTGGAGCTCAATTTGTCGAGACCCCTGCTTCGGGCTTGCGAGAAATTGGGCTATGTTAAGCCCACGCCAATTCAGGCAGCTTGCATTCCACTGGCCTTGTCTGGGAGGGATATATGCGGAAGTGCCATTACTGGTTCCGGGAAGACGGCTGCTTTTGCGTTACCTACACTGGAGAGATTATTGTTTCGCCCCAAGCGGGTGCCTGCCATAAGGGTCCTTGTTCTGACTCCTGCCAGAGAACTGGCGGTTCAGGTTCATAGTATGATTGAGAAACTTGCTCAATTTACTGATATCAGATGTTGCCTGGTTGTTGGAGGCCTTTCACTAAAGGTTCAAGAAGCAGCCTTGAGATCCATGCCGGATATCGTTGTGGCTACCCCGGGACGCATTATAGATCATTTACGAAATTCTATGTCTGTGGATTTGGATGATCTTGCTGTTCTAATTCTTGATGAGGCAGATCGCCTGCTGGAAGTTGGGTTTAGTGCTGAAATTCGTGAGCTGCTTCGAGTATGCCCCAAAAGGAGACAAACCATGCTATTTTCAGCTACGATGACTGAAGAAGTTGATGAACTTGTCCAGCTTTCTCTCACCAAACCACTACGCCTTTCAGCTGACCCATCCGCGAAACGGCCAGTGACACTAACCGAGGAGGTGGTTCGGATACGACGTATGCGTGAACTAAACCAGGAAGCAGTTCTACTTGCATTGTGCACAAAGACATTCAGAGAAAGAGTGATCATATTCAGTGGGACAAAACAGGCCGCACATAGGCTGAAGATCTTATTTGGGTTAGCTGGCTTGAAAGCCGCTGAGCTTCATGGCAATCTTACTCAAGTGCAGCGCCTAGATGCATTGGAACTTTTCAGGAGGGAAAAGGTTGACTTTTTGATTGCAACAGATGTCGCTGCTCGTGGTCTTGACATAATTGGTGTTGAAACAGTTATAAATTATGCATGTCCACGTGATCTTACAAGCTATGTTCATCGAGTGGGTCGTACAGCAAGAGCAGGGAAAGAGGGATGCGCTGTTACTTTCGTGACAGATACTGACCGATCTCTGTTAAAAGCTATTGCAAAAAGAGCTGGTTCAAAGCTGAGGAGTCGAATTGTTGCAGAACAGTCAATTACTAAGTGGTCTCAGATAATTGAGCAAATGGAAGATCAAGTGGCTACAATTCTTCGAGAAGAGAGCGAAGAGACAGCGCTAAGAAGAGCTGAAATGGAAGCAACAAAGGTAGAGAATTTGATTGCACACAGAGATGAAATTTATTCACGCCCCAAAAGGACCTGGATTGTGACTGAAAAGGAGAAGAAGGAGGTAGTGAAGGCATCTAAGGCATCTGGTGAAAGAGAAAAACATTCTGGAAACGAGGTGATCAGTGCCGAACAAGTGGAAGAACTGAAAATGAAAGAAAAGAGGAAACGAGAGCGTGAGAAAAATTTGCCGCGTAAGAAGCGCCGAAATTTGGAAGCCGCTAGAGAAATGTTGGAGGAAGAAAATCAAAATCGAAAGTCAGAAGGTAGTGGAACAACTAAGGACAAGACAGGAGTGAAACTTGTTGACTTGGGTTACCGACGGGCAAAAGCTGTGAAGGCAGCAAATAAGGCAATGGATACTGGCAAGATTGTGAAGAAGGATAGCAAGAAATCTAATCGTCCTCCGCAAAGAACCCCGTCAAGGACAGATGAGATGCAGGACCTGTTCCAGAGTGATATGAGTCAAGGGAAAAAGGATCGAAAAAGCGGTGGCATGGGAAAGAAAAAGTCCAAGAATTCATTTAAAAGCAAGTCGAGGTACAAACGAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

742

Amino Acids

83.32

Weight (kDa)

8.75

Isoelectric Point (pI)

51.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	161 - 331	2.1e-45	DEAD/DEAH box helicase
Helicase_C	PF00271	372 - 477	8.3e-28	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007882)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16630
fragaria_vesca	FvH4_2g38260 FvH4_2g38260 FvH4_2g38260 FvH4_2g38260 FvH4_2g38260 FvH4_2g38260
malus_domestica	MD01G1061100.v1.1 MD15G1442700.v1.1
prunus_persica	Prupe.1G363600_v2.0.a1 Prupe.3G157600_v2.0.a1
pyrus_communis	pycom15g39060
rosa_chinensis	RchiOBHm_Chr6g0303111
rosa_laevigata	RLG00000011107
rosa_multiflora	Rmu_co8350977.1_g000001 Rmu_sc0003721.1_g000002 Rmu_sc0008911.1_g000005
rosa_roxburghii	Rroxscaffold_7G00165010
rosa_rugosa	Rorug06G0319200
rosa_samantha	Rh6AG433100 Rh6BG469600 Rh6CG445400 Rh6DG432200
rosa_wichuraiana	Rw6G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	318, 1364
AccB1I	GGYRCC	1 cut(s)	616
AccBSI	CCGCTC	1 cut(s)	259
AccI	GTMKAC	1 cut(s)	216
AccII	CGCG	3 cut(s)	127, 1043, 1867
AccIII	TCCGGA	1 cut(s)	287
AclWI	GGATC	4 cut(s)	285, 298, 762, 2160
AcoI	YGGCCR	1 cut(s)	1049
AcsI	RAATTY	8 cut(s)	409, 823, 909, 1660, 1683, 1858, 1882, 2191
AcuI	CTGAAG	2 cut(s)	996, 1214
AcvI	CACGTG	1 cut(s)	1382
AfaI	GTAC	2 cut(s)	1417, 2216
AfeI	AGCGCT	1 cut(s)	1623
AfiI	CCNNNNNNNGG	9 cut(s)	268, 447, 448, 519, 613, 1047, 1188, 1885, 2097
AgsI	TTSAA	4 cut(s)	752, 1222, 1355, 1509
AhdI	GACNNNNNGTC	1 cut(s)	394
AjiI	CACGTC	1 cut(s)	396
AjnI	CCWGG	4 cut(s)	417, 720, 1104, 1706
Alw21I	GWGCWC	4 cut(s)	133, 206, 426, 1133
Alw26I	GTCTC	4 cut(s)	430, 937, 1560, 1610
Alw44I	GTGCAC	1 cut(s)	1129
AlwI	GGATC	4 cut(s)	285, 298, 762, 2160
AlwNI	CAGNNNCTG	2 cut(s)	1466, 2119
Ama87I	CYCGRG	2 cut(s)	28, 796
Aor13HI	TCCGGA	1 cut(s)	287
Aor51HI	AGCGCT	1 cut(s)	1623
AoxI	GGCC	5 cut(s)	3, 510, 733, 1049, 1179
ApaLI	GTGCAC	1 cut(s)	1129
ApeKI	GCWGC	7 cut(s)	494, 566, 758, 919, 1258, 1325, 2015
ApoI	RAATTY	8 cut(s)	409, 823, 909, 1660, 1683, 1858, 1882, 2191
ArsI	GACNNNNNNTTYG	2 cut(s)	429, 461
Asp700I	GAANNNNTTC	2 cut(s)	413, 1113
AspLEI	GCGC	4 cut(s)	1263, 1445, 1624, 1878
AspS9I	GGNCC	4 cut(s)	4, 631, 1704, 2116
AsuC2I	CCSGG	4 cut(s)	269, 556, 797, 798
AsuHPI	GGTGA	3 cut(s)	1000, 1775, 1804
AsuII	TTCGAA	1 cut(s)	21
AvaI	CYCGRG	2 cut(s)	28, 796
AvaII	GGWCC	3 cut(s)	631, 1704, 2116
BaeGI	GKGCMC	1 cut(s)	1133
BamHI	GGATCC	1 cut(s)	290
BanI	GGYRCC	1 cut(s)	616
BanII	GRGCYC	1 cut(s)	426
BarI	GAAGNNNNNNTAC	4 cut(s)	529, 561, 1101, 1133
BauI	CACGAG	1 cut(s)	1329
BbrPI	CACGTG	1 cut(s)	1382
BbsI	GAAGAC	3 cut(s)	69, 342, 566
Bbv12I	GWGCWC	4 cut(s)	133, 206, 426, 1133
BbvCI	CCTCAGC	1 cut(s)	1514
BbvI	GCAGC	7 cut(s)	506, 553, 770, 906, 1270, 1312, 2027
BccI	CCATC	1 cut(s)	1045
BceAI	ACGGC	3 cut(s)	406, 579, 1064
BcgI	CGANNNNNNTGC	4 cut(s)	329, 363, 424, 458
BciT130I	CCWGG	4 cut(s)	419, 722, 1106, 1708
BciVI	GTATCC	2 cut(s)	1074, 2026
BclI	TGATCA	2 cut(s)	1155, 1794
BcnI	CCSGG	4 cut(s)	269, 556, 797, 798
BcoDI	GTCTC	4 cut(s)	430, 937, 1560, 1610
BfaI	CTAG	3 cut(s)	282, 1265, 1896
BfoI	RGCGCY	3 cut(s)	1264, 1625, 1879
BfuI	GTATCC	2 cut(s)	1074, 2026
BglII	AGATCT	1 cut(s)	1197
BlpI	GCTNAGC	1 cut(s)	1230
Bme1390I	CCNGG	8 cut(s)	269, 419, 556, 722, 797, 798, 1106, 1708
Bme18I	GGWCC	3 cut(s)	631, 1704, 2116
BmeRI	GACNNNNNGTC	1 cut(s)	394
BmeT110I	CYCGRG	2 cut(s)	28, 796
BmgBI	CACGTC	1 cut(s)	396
BmgT120I	GGNCC	4 cut(s)	4, 631, 1704, 2116
BmiI	GGNNCC	5 cut(s)	6, 292, 553, 618, 632
BmrFI	CCNGG	8 cut(s)	269, 419, 556, 722, 797, 798, 1106, 1708
BmsI	GCATC	5 cut(s)	1258, 1430, 1757, 1766, 2100
BpiI	GAAGAC	3 cut(s)	69, 342, 566
BpmI	CTGGAG	2 cut(s)	440, 608
Bpu10I	CCTNAGC	1 cut(s)	1514
Bpu1102I	GCTNAGC	1 cut(s)	1230
Bpu14I	TTCGAA	1 cut(s)	21
BpuEI	CTTGAG	2 cut(s)	784, 1236
BpuMI	CCSGG	4 cut(s)	269, 556, 797, 798
BsaAI	YACGTR	1 cut(s)	1382
BsaBI	GATNNNNATC	1 cut(s)	295
BsaI	GGTCTC	2 cut(s)	430, 1560
BsaJI	CCNNGG	4 cut(s)	149, 795, 796, 1065
BsaWI	WCCGGW	1 cut(s)	287
BsaXI	ACNNNNNCTCC	2 cut(s)	212, 242
Bsc4I	CCNNNNNNNGG	9 cut(s)	268, 447, 448, 519, 613, 1047, 1188, 1885, 2097
Bse1I	ACTGG	6 cut(s)	513, 553, 591, 663, 1052, 2041
Bse3DI	GCAATG	1 cut(s)	2034
Bse8I	GATNNNNATC	1 cut(s)	295
BseAI	TCCGGA	1 cut(s)	287
BseBI	CCWGG	4 cut(s)	419, 722, 1106, 1708
BseDI	CCNNGG	4 cut(s)	149, 795, 796, 1065
BseGI	GGATG	4 cut(s)	383, 850, 1037, 1445
BseJI	GATNNNNATC	1 cut(s)	295
BseLI	CCNNNNNNNGG	9 cut(s)	268, 447, 448, 519, 613, 1047, 1188, 1885, 2097
BseMI	GCAATG	1 cut(s)	2034
BseMII	CTCAG	5 cut(s)	84, 351, 1221, 1505, 1571
BseNI	ACTGG	6 cut(s)	513, 553, 591, 663, 1052, 2041
BseRI	GAGGAG	2 cut(s)	1082, 1531
BseSI	GKGCMC	1 cut(s)	1133
BseXI	GCAGC	7 cut(s)	506, 553, 770, 906, 1270, 1312, 2027
BsgI	GTGCAG	1 cut(s)	1277
Bsh1236I	CGCG	3 cut(s)	127, 1043, 1867
BshFI	GGCC	5 cut(s)	5, 512, 735, 1051, 1181
BshNI	GGYRCC	1 cut(s)	616
BsiHKAI	GWGCWC	4 cut(s)	133, 206, 426, 1133
BsiHKCI	CYCGRG	2 cut(s)	28, 796
BsiSI	CCGG	6 cut(s)	199, 269, 288, 555, 776, 797
BslFI	GGGAC	4 cut(s)	167, 239, 813, 1183
BslI	CCNNNNNNNGG	9 cut(s)	268, 447, 448, 519, 613, 1047, 1188, 1885, 2097
BsmAI	GTCTC	4 cut(s)	430, 937, 1560, 1610
BsmFI	GGGAC	4 cut(s)	167, 239, 813, 1183
BsmI	GAATGC	1 cut(s)	501
BsnI	GGCC	5 cut(s)	5, 512, 735, 1051, 1181
Bso31I	GGTCTC	2 cut(s)	430, 1560
BsoBI	CYCGRG	2 cut(s)	28, 796
Bsp119I	TTCGAA	1 cut(s)	21
Bsp1286I	GDGCHC	4 cut(s)	133, 206, 426, 1133
Bsp13I	TCCGGA	1 cut(s)	287
Bsp1720I	GCTNAGC	1 cut(s)	1230
Bsp19I	CCATGG	1 cut(s)	149
BspANI	GGCC	5 cut(s)	5, 512, 735, 1051, 1181
BspCNI	CTCAG	5 cut(s)	83, 352, 1222, 1506, 1570
BspEI	TCCGGA	1 cut(s)	287
BspFNI	CGCG	3 cut(s)	127, 1043, 1867
BspLI	GGNNCC	5 cut(s)	6, 292, 553, 618, 632
BspPI	GGATC	4 cut(s)	285, 298, 762, 2160
BspQI	GCTCTTC	1 cut(s)	1624
BspT104I	TTCGAA	1 cut(s)	21
BspT107I	GGYRCC	1 cut(s)	616
BspTNI	GGTCTC	2 cut(s)	430, 1560
BsrBI	CCGCTC	1 cut(s)	259
BsrDI	GCAATG	1 cut(s)	2034
BsrI	ACTGG	6 cut(s)	513, 553, 591, 663, 1052, 2041
BssECI	CCNNGG	4 cut(s)	149, 795, 796, 1065
BssSI	CACGAG	1 cut(s)	1329
BssT1I	CCWWGG	1 cut(s)	149
Bst2BI	CACGAG	1 cut(s)	1329
Bst2UI	CCWGG	4 cut(s)	419, 722, 1106, 1708
Bst4CI	ACNGT	3 cut(s)	178, 1360, 1539
Bst6I	CTCTTC	3 cut(s)	1599, 1608, 1624
BstBAI	YACGTR	1 cut(s)	1382
BstBI	TTCGAA	1 cut(s)	21
BstDEI	CTNAG	9 cut(s)	70, 360, 1230, 1514, 1548, 1557, 1625, 1752, 1953
BstDSI	CCRYGG	1 cut(s)	149
BstEII	GGTNACC	1 cut(s)	1988
BstF5I	GGATG	4 cut(s)	383, 850, 1037, 1445
BstFNI	CGCG	3 cut(s)	127, 1043, 1867
BstH2I	RGCGCY	3 cut(s)	1264, 1625, 1879
BstHHI	GCGC	4 cut(s)	1263, 1445, 1624, 1878
BstMAI	GTCTC	4 cut(s)	430, 937, 1560, 1610
BstMWI	GCNNNNNNNGC	7 cut(s)	356, 572, 1499, 1754, 1873, 2012, 2024
BstNI	CCWGG	4 cut(s)	419, 722, 1106, 1708
BstNSI	RCATGY	1 cut(s)	1377
BstPI	GGTNACC	1 cut(s)	1988
BstSCI	CCNGG	8 cut(s)	267, 417, 554, 720, 795, 796, 1104, 1706
BstSLI	GKGCMC	1 cut(s)	1133
BstUI	CGCG	3 cut(s)	127, 1043, 1867
BstV1I	GCAGC	7 cut(s)	506, 553, 770, 906, 1270, 1312, 2027
BstV2I	GAAGAC	3 cut(s)	69, 342, 566
BstX2I	RGATCY	3 cut(s)	290, 767, 1197
BstXI	CCANNNNNNTGG	1 cut(s)	658
BstYI	RGATCY	3 cut(s)	290, 767, 1197
BsuI	GTATCC	2 cut(s)	1074, 2026
BsuRI	GGCC	5 cut(s)	5, 512, 735, 1051, 1181
BtgI	CCRYGG	1 cut(s)	149
BtgZI	GCGATG	1 cut(s)	48
BtrI	CACGTC	1 cut(s)	396
BtsCI	GGATG	4 cut(s)	383, 850, 1037, 1445
BtsIMutI	CAGTG	5 cut(s)	506, 584, 1059, 1171, 1804
CaiI	CAGNNNCTG	2 cut(s)	1466, 2119
CfoI	GCGC	4 cut(s)	1263, 1445, 1624, 1878
Cfr13I	GGNCC	4 cut(s)	4, 631, 1704, 2116
Cfr9I	CCCGGG	1 cut(s)	796
CseI	GACGC	3 cut(s)	99, 284, 810
Csp6I	GTAC	2 cut(s)	1416, 2215
CspCI	CAANNNNNGTGG	2 cut(s)	495, 530
CviAII	CATG	7 cut(s)	150, 404, 772, 952, 1238, 1374, 2170
CviQI	GTAC	2 cut(s)	1416, 2215
DdeI	CTNAG	9 cut(s)	70, 360, 1230, 1514, 1548, 1557, 1625, 1752, 1953
DraI	TTTAAA	1 cut(s)	2200
DriI	GACNNNNNGTC	1 cut(s)	394
EaeI	YGGCCR	1 cut(s)	1049
Eam1104I	CTCTTC	3 cut(s)	1599, 1608, 1624
Eam1105I	GACNNNNNGTC	1 cut(s)	394
EarI	CTCTTC	3 cut(s)	1599, 1608, 1624
Ecl136II	GAGCTC	1 cut(s)	424
Eco130I	CCWWGG	1 cut(s)	149
Eco147I	AGGCCT	1 cut(s)	735
Eco24I	GRGCYC	1 cut(s)	426
Eco31I	GGTCTC	2 cut(s)	430, 1560
Eco32I	GATATC	2 cut(s)	709, 781
Eco47I	GGWCC	3 cut(s)	631, 1704, 2116
Eco47III	AGCGCT	1 cut(s)	1623
Eco53kI	GAGCTC	1 cut(s)	424
Eco57I	CTGAAG	2 cut(s)	996, 1214
Eco72I	CACGTG	1 cut(s)	1382
Eco88I	CYCGRG	2 cut(s)	28, 796
Eco91I	GGTNACC	1 cut(s)	1988
EcoICRI	GAGCTC	1 cut(s)	424
EcoO109I	RGGNCCY	3 cut(s)	631, 1704, 2116
EcoO65I	GGTNACC	1 cut(s)	1988
EcoRI	GAATTC	2 cut(s)	409, 2191
EcoRII	CCWGG	4 cut(s)	417, 720, 1104, 1706
EcoRV	GATATC	2 cut(s)	709, 781
EcoT14I	CCWWGG	1 cut(s)	149
EcoT22I	ATGCAT	2 cut(s)	1273, 1375
EcoT38I	GRGCYC	1 cut(s)	426
ErhI	CCWWGG	1 cut(s)	149
FaeI	CATG	7 cut(s)	153, 407, 775, 955, 1241, 1377, 2173
FalI	AAGNNNNNCTT	4 cut(s)	359, 391, 1286, 1318
FaqI	GGGAC	4 cut(s)	167, 239, 813, 1183
FatI	CATG	7 cut(s)	149, 403, 771, 951, 1237, 1373, 2169
FauI	CCCGC	4 cut(s)	203, 245, 264, 606
FbaI	TGATCA	2 cut(s)	1155, 1794
FblI	GTMKAC	1 cut(s)	216
FokI	GGATG	4 cut(s)	370, 857, 1024, 1452
FriOI	GRGCYC	1 cut(s)	426
FspBI	CTAG	3 cut(s)	282, 1265, 1896
GlaI	GCGC	4 cut(s)	1262, 1444, 1623, 1877
GsuI	CTGGAG	2 cut(s)	440, 608
HaeII	RGCGCY	3 cut(s)	1264, 1625, 1879
HaeIII	GGCC	5 cut(s)	5, 512, 735, 1051, 1181
HapII	CCGG	6 cut(s)	199, 269, 288, 555, 776, 797
HgaI	GACGC	3 cut(s)	99, 284, 810
HhaI	GCGC	4 cut(s)	1263, 1445, 1624, 1878
Hin1II	CATG	7 cut(s)	153, 407, 775, 955, 1241, 1377, 2173
Hin6I	GCGC	4 cut(s)	1261, 1443, 1622, 1876
HinP1I	GCGC	4 cut(s)	1261, 1443, 1622, 1876
HincII	GTYRAC	3 cut(s)	217, 1300, 1981
HindII	GTYRAC	3 cut(s)	217, 1300, 1981
HinfI	GANTC	4 cut(s)	296, 643, 1519, 2137
HpaII	CCGG	6 cut(s)	199, 269, 288, 555, 776, 797
HphI	GGTGA	3 cut(s)	1000, 1775, 1804
Hpy166II	GTNNAC	6 cut(s)	217, 1097, 1131, 1300, 1379, 1981
Hpy8I	GTNNAC	6 cut(s)	217, 1097, 1131, 1300, 1379, 1981
Hpy99I	CGWCG	8 cut(s)	182, 218, 221, 278, 392, 400, 1089, 1998
HpyAV	CCTTC	5 cut(s)	1726, 1738, 1931, 2005, 2047
HpyCH4III	ACNGT	3 cut(s)	178, 1360, 1539
HpyCH4IV	ACGT	3 cut(s)	395, 1087, 1381
HpyF10VI	GCNNNNNNNGC	7 cut(s)	356, 572, 1499, 1754, 1873, 2012, 2024
HpyF3I	CTNAG	9 cut(s)	70, 360, 1230, 1514, 1548, 1557, 1625, 1752, 1953
HpySE526I	ACGT	3 cut(s)	395, 1087, 1381
Hsp92II	CATG	7 cut(s)	153, 407, 775, 955, 1241, 1377, 2173
HspAI	GCGC	4 cut(s)	1261, 1443, 1622, 1876
Kpn2I	TCCGGA	1 cut(s)	287
Ksp22I	TGATCA	2 cut(s)	1155, 1794
LguI	GCTCTTC	1 cut(s)	1624
LmnI	GCTCC	4 cut(s)	201, 264, 290, 421
Lsp1109I	GCAGC	7 cut(s)	506, 553, 770, 906, 1270, 1312, 2027
LweI	GCATC	5 cut(s)	1258, 1430, 1757, 1766, 2100
MaeI	CTAG	3 cut(s)	282, 1265, 1896
MaeII	ACGT	3 cut(s)	395, 1087, 1381
MaeIII	GTNAC	6 cut(s)	576, 1054, 1447, 1456, 1714, 1988
MbiI	CCGCTC	1 cut(s)	259
MflI	RGATCY	3 cut(s)	290, 767, 1197
MhlI	GDGCHC	4 cut(s)	133, 206, 426, 1133
MlyI	GAGTC	3 cut(s)	637, 1528, 2146
MmeI	TCCRAC	3 cut(s)	411, 709, 1887
Mph1103I	ATGCAT	2 cut(s)	1273, 1375
MroI	TCCGGA	1 cut(s)	287
MroXI	GAANNNNTTC	2 cut(s)	413, 1113
MseI	TTAA	3 cut(s)	474, 1481, 2199
MslI	CAYNNNNRTG	1 cut(s)	1677
MspA1I	CMGCKG	2 cut(s)	1031, 1229
MspI	CCGG	6 cut(s)	199, 269, 288, 555, 776, 797
MspR9I	CCNGG	8 cut(s)	269, 419, 556, 722, 797, 798, 1106, 1708
Mva1269I	GAATGC	1 cut(s)	501
MvaI	CCWGG	4 cut(s)	419, 722, 1106, 1708
MvnI	CGCG	3 cut(s)	127, 1043, 1867
MwoI	GCNNNNNNNGC	7 cut(s)	356, 572, 1499, 1754, 1873, 2012, 2024
NciI	CCSGG	4 cut(s)	269, 556, 797, 798
NcoI	CCATGG	1 cut(s)	149
NlaIII	CATG	7 cut(s)	153, 407, 775, 955, 1241, 1377, 2173
NlaIV	GGNNCC	5 cut(s)	6, 292, 553, 618, 632
NmuCI	GTSAC	3 cut(s)	1054, 1456, 1714
NsiI	ATGCAT	2 cut(s)	1273, 1375
NspI	RCATGY	1 cut(s)	1377
NspV	TTCGAA	1 cut(s)	21
PceI	AGGCCT	1 cut(s)	735
PciSI	GCTCTTC	1 cut(s)	1624
PcsI	WCGNNNNNNNCGW	4 cut(s)	404, 1084, 1090, 1411
PctI	GAATGC	1 cut(s)	501
PdmI	GAANNNNTTC	2 cut(s)	413, 1113
PfeI	GAWTC	1 cut(s)	296
PfoI	TCCNGGA	2 cut(s)	417, 554
PleI	GAGTC	3 cut(s)	637, 1527, 2145
PmaCI	CACGTG	1 cut(s)	1382
PmlI	CACGTG	1 cut(s)	1382
PpsI	GAGTC	3 cut(s)	637, 1527, 2145
Ppu21I	YACGTR	1 cut(s)	1382
PpuMI	RGGWCCY	3 cut(s)	631, 1704, 2116
PsiI	TTATAA	2 cut(s)	318, 1364
Psp124BI	GAGCTC	1 cut(s)	426
Psp5II	RGGWCCY	3 cut(s)	631, 1704, 2116
Psp6I	CCWGG	4 cut(s)	417, 720, 1104, 1706
PspCI	CACGTG	1 cut(s)	1382
PspEI	GGTNACC	1 cut(s)	1988
PspGI	CCWGG	4 cut(s)	417, 720, 1104, 1706
PspN4I	GGNNCC	5 cut(s)	6, 292, 553, 618, 632
PspPI	GGNCC	4 cut(s)	4, 631, 1704, 2116
PspPPI	RGGWCCY	3 cut(s)	631, 1704, 2116
PstNI	CAGNNNCTG	2 cut(s)	1466, 2119
PsuI	RGATCY	3 cut(s)	290, 767, 1197
PvuII	CAGCTG	1 cut(s)	1031
RsaI	GTAC	2 cut(s)	1417, 2216
RsaNI	GTAC	2 cut(s)	1416, 2215
RseI	CAYNNNNRTG	1 cut(s)	1677
SacI	GAGCTC	1 cut(s)	426
SalI	GTCGAC	1 cut(s)	215
SapI	GCTCTTC	1 cut(s)	1624
SaqAI	TTAA	3 cut(s)	474, 1481, 2199
Sau96I	GGNCC	4 cut(s)	4, 631, 1704, 2116
SchI	GAGTC	3 cut(s)	637, 1528, 2146
ScrFI	CCNGG	8 cut(s)	269, 419, 556, 722, 797, 798, 1106, 1708
SduI	GDGCHC	4 cut(s)	133, 206, 426, 1133
SfaNI	GCATC	5 cut(s)	1258, 1430, 1757, 1766, 2100
SfuI	TTCGAA	1 cut(s)	21
SgrDI	CGTCGACG	1 cut(s)	215
SinI	GGWCC	3 cut(s)	631, 1704, 2116
SmaI	CCCGGG	1 cut(s)	798
SmiMI	CAYNNNNRTG	1 cut(s)	1677
SmlI	CTYRAG	2 cut(s)	763, 1251
SmoI	CTYRAG	2 cut(s)	763, 1251
SseBI	AGGCCT	1 cut(s)	735
SspI	AATATT	1 cut(s)	44
SspMI	CTAG	3 cut(s)	282, 1265, 1896
SstI	GAGCTC	1 cut(s)	426
StuI	AGGCCT	1 cut(s)	735
StyD4I	CCNGG	8 cut(s)	267, 417, 554, 720, 795, 796, 1104, 1706
StyI	CCWWGG	1 cut(s)	149
TaaI	ACNGT	3 cut(s)	178, 1360, 1539
TaiI	ACGT	3 cut(s)	398, 1090, 1384
TaqII	GACCGA	1 cut(s)	1485
TauI	GCSGC	5 cut(s)	359, 1184, 1229, 1867, 1895
TfiI	GAWTC	1 cut(s)	296
Tru1I	TTAA	3 cut(s)	474, 1481, 2199
Tru9I	TTAA	3 cut(s)	474, 1481, 2199
TscAI	CASTG	5 cut(s)	513, 591, 1059, 1171, 1804
TseFI	GTSAC	3 cut(s)	1054, 1456, 1714
TseI	GCWGC	7 cut(s)	494, 566, 758, 919, 1258, 1325, 2015
Tsp45I	GTSAC	3 cut(s)	1054, 1456, 1714
TspGWI	ACGGA	2 cut(s)	202, 241
TspMI	CCCGGG	1 cut(s)	796
TspRI	CASTG	5 cut(s)	513, 591, 1059, 1171, 1804
VneI	GTGCAC	1 cut(s)	1129
VpaK11BI	GGWCC	3 cut(s)	631, 1704, 2116
XapI	RAATTY	8 cut(s)	409, 823, 909, 1660, 1683, 1858, 1882, 2191
XceI	RCATGY	1 cut(s)	1377
XmaI	CCCGGG	1 cut(s)	796
XmiI	GTMKAC	1 cut(s)	216
XmnI	GAANNNNTTC	2 cut(s)	413, 1113
XspI	CTAG	3 cut(s)	282, 1265, 1896
Zsp2I	ATGCAT	2 cut(s)	1273, 1375

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.