Rw1G040130

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015111
Physical Location & Seq
Forward (+)
67524727 .. 67526124
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G040130.1

Sequence Viewer

Length: 1398 bp
ATGATCAAGCTTTGTAGAATCCAACTACCCAAAGCCACCAGAATTTCAGACCTTCTCAACCGAAGCTGCTTGACTACAACGAAAACTGCTGGAAATTTGGATGTACAATCTCCTCCAAGTATCCCGGACCCGGAAACACTAGCAACCCAGTTGTCCAATTGCACTAGTTTATCTGAACTGAACCAAATCTATGCCCACATTATCAGAACCCAATTCCTACGTCTCCACCCAGCCCCATTTCACTGGAACAATATCATAAGGTCATACACCAGACGCGATGCTCCTACCAAGTCGCTCTTTGTTTACGGTTCCATGTCACGCGCCGGTGTTTTACCCGACTGCTACACCATCCCCATTGTTTTGAAAACACTGTGTCAGCTTTTTGCTGTGGAGATCGGCAGGCAGCTTCATTCTGTGGCTGTGAGGATTGGGCTCGACTCAAATGAGTTCTGTGAGAGTGGGTTTATTAACTTGTATTCCAAGGCGGGAGAGTTTGAGAATGCACGGAACATGTTCGATCAAAACCCTGAGAGAAAGCTGGGTTCTTGGAATGCCCTCATTGCGGGGCTTTCGCAAGGCGGGCGTGCTAGGGAAGCTGTAGAGATGTTTATAGAGCTTAGGAAATGCGGGTTACTGCCGGATGATGTGACCATGGTTAGTGTCACGTCAGCTTGTGGTGGTCTGGGTGACTTGGGGTTGGCTCTTCAGTTGCATAAGTGTGTTTATCAAGCTGAAATCGTTGCGGAGAAATCGGATGTGCTGATGTTGAATTCGCTTGTTGATATGTATGGGAAATGTGGGAGGATGGACTTGGCTCACAGGGTGTTTTCGAGGATGGAGCAACAGAATGTGTCGTCGTGGACGTCGATGATTGTGGGGTATGGGATGCACGGGCATGTGGATGAGGCGCTGGAATGCTTTCGGTGCATGAGGGAGGCGGGAGTGAGGCCGAACCATGTGACCTTTGTTGGGGTGCTCAGTGCATGTGTTCATGGGGGGACAGTCCAAGAGGGAAGATATTACTTTGAAATGATGAACAAGGATTATGGGATAAACCCTCGCTTGCAGCATTATGGATGCATGGTGGATCTTCTTGGCAGAGCGGGGTTGCTTGAGGAGGCGAGGAAAATGGTGGAAGACATGCCGATGAAAGCAAATTCGGCAGTCTGGGGCTGCCTGATGGGAGCCTGTGAGAAATATGGGAATGTGAAGATGGGGGAGTGGGTGGCTAAGCATTTGCAAGAACTAGAGCCATGGAATGATGGGGCTTTTGTGGTTTTATCTAATATCTATGCCAGTAGGGGTTTATGGAAAGAGGTTGAGAGGCTAAGACGGATTATGCACCAGAGAAAGCTTGCTAAGGTTCCTGGTTATAGCTTGGCAGGAAGTGCAGATTGA

Protein Analysis

465

Amino Acids

51.96

Weight (kDa)

8.26

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 81 - 126 1e-05 PPR repeat family
PPR_2 PF13041 182 - 223 1e-06 PPR repeat family
PPR PF01535 182 - 211 4.7e-06 PPR repeat
PPR_2 PF13041 283 - 330 2.2e-10 PPR repeat family
PPR PF01535 286 - 315 3.3e-08 PPR repeat
E_motif PF20431 400 - 459 6.3e-16 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77170
fragaria_vesca FvH4_7g33970
malus_domestica MD01G1235900.v1.1
prunus_persica Prupe.2G326000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0384201
rosa_laevigata RLG00000026060
rosa_multiflora Rmu_co8317187.1_g000001 Rmu_sc0005139.1_g000005 Rmu_sc0014578.1_g000005
rosa_roxburghii Rroxscaffold_4G00276860
rosa_rugosa Rorug01G0445700
rosa_samantha Rh1AG471200 Rh1BG429000 Rh1CG441600 Rh1DG461700
rosa_wichuraiana Rw1G040130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 866
AccBSI CCGCTC 1 cut(s) 1103
AccII CGCG 2 cut(s) 276, 321
AciI CCGC 7 cut(s) 485, 563, 579, 627, 743, 938, 1103
AclWI GGATC 1 cut(s) 1095
AcsI RAATTY 4 cut(s) 42, 94, 769, 1156
AcuI CTGAAG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 863
AdeI CACNNNGTG 1 cut(s) 823
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 3 cut(s) 130, 562, 969
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 3 cut(s) 364, 769, 1028
AhlI ACTAGT 1 cut(s) 164
AjiI CACGTC 1 cut(s) 666
AjnI CCWGG 1 cut(s) 1366
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 1095
AoxI GGCC 1 cut(s) 947
ApeKI GCWGC 4 cut(s) 66, 403, 1066, 1173
ApoI RAATTY 4 cut(s) 42, 94, 769, 1156
Asp700I GAANNNNTTC 2 cut(s) 512, 918
AspLEI GCGC 2 cut(s) 323, 910
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 2 cut(s) 125, 131
AsuHPI GGTGA 1 cut(s) 698
AvaII GGWCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 1143
Bbv12I GWGCWC 1 cut(s) 978
BbvI GCAGC 4 cut(s) 53, 415, 1078, 1160
BccI CCATC 6 cut(s) 356, 799, 829, 1174, 1207, 1256
BciT130I CCWGG 1 cut(s) 1368
BciVI GTATCC 1 cut(s) 131
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 125, 131
BcoDI GTCTC 1 cut(s) 227
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 4 cut(s) 140, 165, 588, 1247
BfmI CTRYAG 1 cut(s) 597
BfoI RGCGCY 1 cut(s) 911
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 4 cut(s) 67, 404, 1067, 1174
BlpI GCTNAGC 1 cut(s) 1230
BlsI GCNGC 4 cut(s) 68, 405, 1068, 1175
Bme1390I CCNGG 3 cut(s) 125, 131, 1368
Bme18I GGWCC 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 666
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 4 cut(s) 129, 310, 1186, 1365
BmrFI CCNGG 3 cut(s) 125, 131, 1368
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 3 cut(s) 268, 876, 1067
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 1143
Bpu10I CCTNAGC 2 cut(s) 617, 1359
Bpu1102I GCTNAGC 1 cut(s) 1230
BpuEI CTTGAG 1 cut(s) 1133
BpuMI CCSGG 2 cut(s) 125, 131
BsaHI GRCGYC 1 cut(s) 863
BsaJI CCNNGG 3 cut(s) 480, 651, 1253
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 562, 969
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 3 cut(s) 148, 248, 1296
Bse3DI GCAATG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 1368
BseDI CCNNGG 3 cut(s) 480, 651, 1253
BseGI GGATG 9 cut(s) 106, 348, 646, 760, 810, 840, 891, 907, 1082
BseLI CCNNNNNNNGG 3 cut(s) 130, 562, 969
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 2 cut(s) 519, 991
BseNI ACTGG 3 cut(s) 148, 248, 1296
BseRI GAGGAG 2 cut(s) 102, 1130
BseXI GCAGC 4 cut(s) 53, 415, 1078, 1160
BseYI CCCAGC 2 cut(s) 229, 538
Bsh1236I CGCG 2 cut(s) 276, 321
BshFI GGCC 1 cut(s) 949
BsiHKAI GWGCWC 1 cut(s) 978
BsiSI CCGG 4 cut(s) 125, 131, 324, 638
BslFI GGGAC 1 cut(s) 1012
BslI CCNNNNNNNGG 3 cut(s) 130, 562, 969
BsmAI GTCTC 1 cut(s) 227
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 1012
BsmI GAATGC 3 cut(s) 505, 556, 920
BsnI GGCC 1 cut(s) 949
Bsp1286I GDGCHC 2 cut(s) 435, 978
Bsp1407I TGTACA 1 cut(s) 103
Bsp143I GATC 4 cut(s) 3, 393, 517, 1087
Bsp1720I GCTNAGC 1 cut(s) 1230
Bsp19I CCATGG 2 cut(s) 651, 1253
BspACI CCGC 7 cut(s) 485, 563, 579, 627, 743, 938, 1103
BspANI GGCC 1 cut(s) 949
BspCNI CTCAG 2 cut(s) 520, 990
BspFNI CGCG 2 cut(s) 276, 321
BspLI GGNNCC 4 cut(s) 129, 310, 1186, 1365
BspPI GGATC 1 cut(s) 1095
BspQI GCTCTTC 1 cut(s) 708
BsrBI CCGCTC 1 cut(s) 1103
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 103
BsrI ACTGG 3 cut(s) 148, 248, 1296
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 480, 651, 1253
BssMI GATC 4 cut(s) 3, 393, 517, 1087
BssNI GRCGYC 1 cut(s) 863
BssT1I CCWWGG 3 cut(s) 480, 651, 1253
Bst2UI CCWGG 1 cut(s) 1368
Bst4CI ACNGT 3 cut(s) 308, 372, 1003
Bst6I CTCTTC 1 cut(s) 708
BstACI GRCGYC 1 cut(s) 863
BstAPI GCANNNNNTGC 1 cut(s) 1388
BstAUI TGTACA 1 cut(s) 103
BstC8I GCNNGC 5 cut(s) 401, 581, 585, 1064, 1356
BstDEI CTNAG 6 cut(s) 528, 617, 977, 1230, 1328, 1359
BstDSI CCRYGG 2 cut(s) 651, 1253
BstF5I GGATG 9 cut(s) 106, 348, 646, 760, 810, 840, 891, 907, 1082
BstFNI CGCG 2 cut(s) 276, 321
BstH2I RGCGCY 1 cut(s) 911
BstHHI GCGC 2 cut(s) 323, 910
BstKTI GATC 4 cut(s) 6, 396, 520, 1090
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 3, 393, 517, 1087
BstMWI GCNNNNNNNGC 6 cut(s) 560, 580, 593, 924, 1160, 1388
BstNI CCWGG 1 cut(s) 1368
BstNSI RCATGY 4 cut(s) 514, 899, 987, 1144
BstSCI CCNGG 3 cut(s) 123, 129, 1366
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 2 cut(s) 276, 321
BstV1I GCAGC 4 cut(s) 53, 415, 1078, 1160
BstV2I GAAGAC 1 cut(s) 1143
BstX2I RGATCY 1 cut(s) 1087
BstXI CCANNNNNNTGG 1 cut(s) 243
BstYI RGATCY 1 cut(s) 1087
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 949
BtgI CCRYGG 2 cut(s) 651, 1253
BtgZI GCGATG 1 cut(s) 291
BtrI CACGTC 1 cut(s) 666
BtsCI GGATG 9 cut(s) 106, 348, 646, 760, 810, 840, 891, 907, 1082
BtsIMutI CAGTG 3 cut(s) 241, 368, 985
Cac8I GCNNGC 5 cut(s) 401, 581, 585, 1064, 1356
CfoI GCGC 2 cut(s) 323, 910
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 282
Csp6I GTAC 1 cut(s) 104
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 6 cut(s) 528, 617, 977, 1230, 1328, 1359
DpnI GATC 4 cut(s) 5, 395, 519, 1089
DpnII GATC 4 cut(s) 3, 393, 517, 1087
DraIII CACNNNGTG 1 cut(s) 823
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
Eco130I CCWWGG 3 cut(s) 480, 651, 1253
Eco24I GRGCYC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 689
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 1 cut(s) 1366
EcoT14I CCWWGG 3 cut(s) 480, 651, 1253
EcoT22I ATGCAT 1 cut(s) 1082
EcoT38I GRGCYC 1 cut(s) 435
ErhI CCWWGG 3 cut(s) 480, 651, 1253
Esp3I CGTCTC 1 cut(s) 227
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 1 cut(s) 1012
FauI CCCGC 6 cut(s) 478, 556, 572, 620, 931, 1096
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 4 cut(s) 67, 404, 1067, 1174
FokI GGATG 9 cut(s) 113, 335, 653, 767, 817, 847, 898, 914, 1089
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 4 cut(s) 67, 404, 1067, 1174
FspBI CTAG 4 cut(s) 140, 165, 588, 1247
GlaI GCGC 2 cut(s) 322, 909
GluI GCNGC 4 cut(s) 67, 404, 1067, 1174
GsaI CCCAGC 2 cut(s) 233, 542
HaeII RGCGCY 1 cut(s) 911
HaeIII GGCC 1 cut(s) 949
HapII CCGG 4 cut(s) 125, 131, 324, 638
HgaI GACGC 1 cut(s) 282
HhaI GCGC 2 cut(s) 323, 910
Hin1I GRCGYC 1 cut(s) 863
Hin6I GCGC 2 cut(s) 321, 908
HinP1I GCGC 2 cut(s) 321, 908
HindIII AAGCTT 2 cut(s) 8, 1352
HinfI GANTC 2 cut(s) 18, 437
HpaII CCGG 4 cut(s) 125, 131, 324, 638
HphI GGTGA 1 cut(s) 698
Hpy166II GTNNAC 2 cut(s) 304, 861
Hpy188I TCNGA 4 cut(s) 49, 175, 206, 754
Hpy8I GTNNAC 2 cut(s) 304, 861
Hpy99I CGWCG 2 cut(s) 859, 868
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 3 cut(s) 308, 372, 1003
HpyCH4IV ACGT 3 cut(s) 220, 665, 863
HpyF10VI GCNNNNNNNGC 6 cut(s) 560, 580, 593, 924, 1160, 1388
HpyF3I CTNAG 6 cut(s) 528, 617, 977, 1230, 1328, 1359
HpySE526I ACGT 3 cut(s) 220, 665, 863
Hsp92I GRCGYC 1 cut(s) 863
HspAI GCGC 2 cut(s) 321, 908
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 393, 517, 1087
LguI GCTCTTC 1 cut(s) 708
LmnI GCTCC 3 cut(s) 286, 838, 1184
Lsp1109I GCAGC 4 cut(s) 53, 415, 1078, 1160
LweI GCATC 3 cut(s) 268, 876, 1067
MaeI CTAG 4 cut(s) 140, 165, 588, 1247
MaeII ACGT 3 cut(s) 220, 665, 863
MaeIII GTNAC 6 cut(s) 315, 630, 646, 661, 686, 958
MalI GATC 4 cut(s) 5, 395, 519, 1089
MbiI CCGCTC 1 cut(s) 1103
MboI GATC 4 cut(s) 3, 393, 517, 1087
MboII GAAGA 5 cut(s) 695, 1026, 1082, 1148, 1222
MfeI CAATTG 1 cut(s) 157
MflI RGATCY 1 cut(s) 1087
MhlI GDGCHC 2 cut(s) 435, 978
MluCI AATT 6 cut(s) 42, 94, 157, 212, 769, 1156
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 1082
MroXI GAANNNNTTC 2 cut(s) 512, 918
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 4 cut(s) 717, 894, 900, 1145
MspI CCGG 4 cut(s) 125, 131, 324, 638
MspR9I CCNGG 3 cut(s) 125, 131, 1368
MunI CAATTG 1 cut(s) 157
Mva1269I GAATGC 3 cut(s) 505, 556, 920
MvaI CCWGG 1 cut(s) 1368
MvnI CGCG 2 cut(s) 276, 321
MwoI GCNNNNNNNGC 6 cut(s) 560, 580, 593, 924, 1160, 1388
NciI CCSGG 2 cut(s) 125, 131
NcoI CCATGG 2 cut(s) 651, 1253
NdeII GATC 4 cut(s) 3, 393, 517, 1087
NlaIV GGNNCC 4 cut(s) 129, 310, 1186, 1365
NmuCI GTSAC 5 cut(s) 315, 646, 661, 686, 958
NsiI ATGCAT 1 cut(s) 1082
NspI RCATGY 4 cut(s) 514, 899, 987, 1144
PciI ACATGT 1 cut(s) 510
PciSI GCTCTTC 1 cut(s) 708
PcsI WCGNNNNNNNCGW 2 cut(s) 860, 863
PctI GAATGC 3 cut(s) 505, 556, 920
PdmI GAANNNNTTC 2 cut(s) 512, 918
PfeI GAWTC 1 cut(s) 18
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 4 cut(s) 68, 405, 1068, 1175
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PscI ACATGT 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 1366
PspFI CCCAGC 2 cut(s) 229, 538
PspGI CCWGG 1 cut(s) 1366
PspN4I GGNNCC 4 cut(s) 129, 310, 1186, 1365
PspPI GGNCC 1 cut(s) 127
PsuI RGATCY 1 cut(s) 1087
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
RseI CAYNNNNRTG 4 cut(s) 717, 894, 900, 1145
SapI GCTCTTC 1 cut(s) 708
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 4 cut(s) 67, 404, 1067, 1174
Sau3AI GATC 4 cut(s) 3, 393, 517, 1087
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 3 cut(s) 125, 131, 1368
SduI GDGCHC 2 cut(s) 435, 978
SfaNI GCATC 3 cut(s) 268, 876, 1067
SfcI CTRYAG 1 cut(s) 597
SgrAI CRCCGGYG 1 cut(s) 323
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 4 cut(s) 717, 894, 900, 1145
SmlI CTYRAG 1 cut(s) 1112
SmoI CTYRAG 1 cut(s) 1112
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 6 cut(s) 42, 94, 157, 212, 769, 1156
SsiI CCGC 7 cut(s) 485, 563, 579, 627, 743, 938, 1103
SspMI CTAG 4 cut(s) 140, 165, 588, 1247
StyD4I CCNGG 3 cut(s) 123, 129, 1366
StyI CCWWGG 3 cut(s) 480, 651, 1253
TaaI ACNGT 3 cut(s) 308, 372, 1003
TaiI ACGT 3 cut(s) 223, 668, 866
TaqI TCGA 4 cut(s) 435, 516, 830, 866
TasI AATT 6 cut(s) 42, 94, 157, 212, 769, 1156
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 3 cut(s) 248, 375, 985
TseFI GTSAC 5 cut(s) 315, 646, 661, 686, 958
TseI GCWGC 4 cut(s) 66, 403, 1066, 1173
Tsp45I GTSAC 5 cut(s) 315, 646, 661, 686, 958
TspDTI ATGAA 4 cut(s) 398, 980, 1049, 1163
TspGWI ACGGA 2 cut(s) 520, 1348
TspRI CASTG 3 cut(s) 248, 375, 985
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 4 cut(s) 42, 94, 769, 1156
XceI RCATGY 4 cut(s) 514, 899, 987, 1144
XmnI GAANNNNTTC 2 cut(s) 512, 918
XspI CTAG 4 cut(s) 140, 165, 588, 1247
ZraI GACGTC 1 cut(s) 864
Zsp2I ATGCAT 1 cut(s) 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.