Rw2G003330

Fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014289
Physical Location & Seq
Forward (+)
3088562 .. 3089674
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003330.1

Sequence Viewer

Length: 741 bp
ATGGCAGCTTCCACACATTGCCTCCCTGTCCTCCTCCTCGCCGTCGCCGCCTTCTTCGTCCTCCATGCTCAAGCCCAAACGGCACCCGCCCCTGCCCCGCCCGGCAAACTTAACTTCACCGGCATCCTAGACAAAGCTGGCCAATACACCACCTTCCTCCGCCTCCTCACCCAAACCCAAGTTGCCACCCAAATCCAAAGCCAGCTCAACTCCTCCACCGAGGGCCTAACCGTCCTTGCACCCACCGACAACGCCTTCACAAACCTCAAAGCCGGTACCTTAAACGACCTCTCCTCCCAACAACAAGTCCAGCTAGTTCTGTACCATGTTCTCCCCAAGTACTACACTCTCGCCAGTCTCCTAACGGTCTCGAACCCCGTGAGAACTCAGTCCTCGTCCCAAAACGGCGGCGGATCTTACGGCCTCAACTTCACCGGATCCGGTGGGAACCAAGTGAATGTGTCGAGCGGGCCGGTGACTACTCAGATCAACAATGCGTTGAGGCAGCAGTTTCCGTTGGCTGTGTACCAAGTTGACAAGGTGTTGTTACCTAATGACCTGTTTAGTGACTCGTCACCGGCTCCTACTCCGACTAAAACTCGGGCCGGAAGCACCAACACGACGACGGCGACAAAGGCCGCGGAGCCATCTGCGGACAGTGCTAATGGTTCGAGCAAGAAGAGCGTGGGATTCGTGGGATTGGCTCTAGGGGTGGTTTTGTTTTTCATGGGAACTAGCTAG

Protein Analysis

246

Amino Acids

25.61

Weight (kDa)

9.27

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 48 - 184 4.4e-22 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0014289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03870 AT5G44130
fragaria_vesca FvH4_1g03420
malus_domestica MD15G1174300.v1.1
prunus_persica Prupe.7G241800_v2.0.a1
pyrus_communis pycom15g15590
rosa_chinensis RchiOBHm_Chr2g0088651
rosa_laevigata RLG00000015972
rosa_multiflora Rmu_sc0006835.1_g000014 Rmu_sc0015442.1_g000011
rosa_roxburghii Rroxscaffold_2G00152430
rosa_rugosa Rorug01G0485600
rosa_samantha Rh2AG041100 Rh2BG039800 Rh2CG040600 Rh2DG040200
rosa_wichuraiana Rw2G003330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 82, 275
AccBSI CCGCTC 1 cut(s) 468
AccII CGCG 1 cut(s) 641
AclWI GGATC 3 cut(s) 421, 432, 445
AcoI YGGCCR 1 cut(s) 139
AfaI GTAC 4 cut(s) 277, 323, 341, 527
AluBI AGCT 5 cut(s) 8, 137, 205, 313, 738
AluI AGCT 5 cut(s) 8, 137, 205, 313, 738
Alw26I GTCTC 2 cut(s) 362, 373
AlwI GGATC 3 cut(s) 421, 432, 445
Ama87I CYCGRG 1 cut(s) 600
AoxI GGCC 6 cut(s) 139, 223, 421, 470, 603, 636
ApeKI GCWGC 2 cut(s) 5, 505
Asp718I GGTACC 1 cut(s) 275
AspS9I GGNCC 3 cut(s) 223, 470, 603
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 5 cut(s) 109, 160, 424, 487, 567
AvaI CYCGRG 1 cut(s) 600
BalI TGGCCA 1 cut(s) 141
BamHI GGATCC 1 cut(s) 437
BanI GGYRCC 2 cut(s) 82, 275
BbvI GCAGC 2 cut(s) 17, 517
BccI CCATC 1 cut(s) 655
BceAI ACGGC 5 cut(s) 26, 96, 421, 436, 642
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 362, 373
BfaI CTAG 5 cut(s) 128, 314, 707, 735, 739
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 5 cut(s) 6, 48, 409, 506, 639
BlsI GCNGC 5 cut(s) 7, 49, 410, 507, 640
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 1 cut(s) 102
BmeT110I CYCGRG 1 cut(s) 600
BmgT120I GGNCC 3 cut(s) 223, 470, 603
BmiI GGNNCC 6 cut(s) 84, 277, 439, 449, 582, 645
BmrFI CCNGG 1 cut(s) 102
BmsI GCATC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 54
BpuMI CCSGG 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 2 cut(s) 219, 639
BsaWI WCCGGW 2 cut(s) 434, 440
BsaXI ACNNNNNCTCC 6 cut(s) 6, 36, 275, 278, 305, 308
Bse118I RCCGGY 4 cut(s) 119, 272, 472, 577
Bse1I ACTGG 1 cut(s) 354
Bse3DI GCAATG 1 cut(s) 16
BseDI CCNNGG 2 cut(s) 219, 639
BseGI GGATG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 401, 497
BseNI ACTGG 1 cut(s) 354
BseRI GAGGAG 5 cut(s) 23, 26, 155, 202, 283
BseXI GCAGC 2 cut(s) 17, 517
Bsh1236I CGCG 1 cut(s) 641
BshFI GGCC 6 cut(s) 141, 225, 423, 472, 605, 638
BshNI GGYRCC 2 cut(s) 82, 275
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 8 cut(s) 102, 120, 273, 435, 441, 473, 578, 606
BslFI GGGAC 1 cut(s) 382
BsmAI GTCTC 2 cut(s) 362, 373
BsmFI GGGAC 1 cut(s) 382
BsnI GGCC 6 cut(s) 141, 225, 423, 472, 605, 638
Bso31I GGTCTC 1 cut(s) 373
BsoBI CYCGRG 1 cut(s) 600
Bsp143I GATC 3 cut(s) 413, 437, 486
BspANI GGCC 6 cut(s) 141, 225, 423, 472, 605, 638
BspCNI CTCAG 2 cut(s) 400, 496
BspFNI CGCG 1 cut(s) 641
BspLI GGNNCC 6 cut(s) 84, 277, 439, 449, 582, 645
BspPI GGATC 3 cut(s) 421, 432, 445
BspQI GCTCTTC 1 cut(s) 674
BspT107I GGYRCC 2 cut(s) 82, 275
BspTNI GGTCTC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 4 cut(s) 119, 272, 472, 577
BsrI ACTGG 1 cut(s) 354
BssAI RCCGGY 4 cut(s) 119, 272, 472, 577
BssECI CCNNGG 2 cut(s) 219, 639
BssMI GATC 3 cut(s) 413, 437, 486
Bst4CI ACNGT 3 cut(s) 232, 367, 659
Bst6I CTCTTC 1 cut(s) 674
BstC8I GCNNGC 3 cut(s) 139, 203, 470
BstDEI CTNAG 2 cut(s) 387, 483
BstDSI CCRYGG 1 cut(s) 639
BstF5I GGATG 1 cut(s) 123
BstFNI CGCG 1 cut(s) 641
BstKTI GATC 3 cut(s) 416, 440, 489
BstMAI GTCTC 2 cut(s) 362, 373
BstMBI GATC 3 cut(s) 413, 437, 486
BstMWI GCNNNNNNNGC 5 cut(s) 47, 80, 635, 659, 681
BstSCI CCNGG 1 cut(s) 100
BstUI CGCG 1 cut(s) 641
BstV1I GCAGC 2 cut(s) 17, 517
BstX2I RGATCY 2 cut(s) 413, 437
BstYI RGATCY 2 cut(s) 413, 437
BsuRI GGCC 6 cut(s) 141, 225, 423, 472, 605, 638
BtgI CCRYGG 1 cut(s) 639
BtsCI GGATG 1 cut(s) 123
BtsIMutI CAGTG 1 cut(s) 664
Cac8I GCNNGC 3 cut(s) 139, 203, 470
Cfr10I RCCGGY 4 cut(s) 119, 272, 472, 577
Cfr13I GGNCC 3 cut(s) 223, 470, 603
Cfr42I CCGCGG 1 cut(s) 642
Csp6I GTAC 4 cut(s) 276, 322, 340, 526
CviAII CATG 3 cut(s) 65, 326, 727
CviQI GTAC 4 cut(s) 276, 322, 340, 526
DdeI CTNAG 2 cut(s) 387, 483
DpnI GATC 3 cut(s) 415, 439, 488
DpnII GATC 3 cut(s) 413, 437, 486
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 674
EarI CTCTTC 1 cut(s) 674
EciI GGCGGA 2 cut(s) 149, 426
Eco31I GGTCTC 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 223
FaeI CATG 3 cut(s) 68, 329, 730
FaiI YATR 3 cut(s) 66, 327, 728
FaqI GGGAC 1 cut(s) 382
FatI CATG 3 cut(s) 64, 325, 726
FauI CCCGC 3 cut(s) 94, 105, 461
Fnu4HI GCNGC 5 cut(s) 6, 48, 409, 506, 639
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 5 cut(s) 6, 48, 409, 506, 639
FspBI CTAG 5 cut(s) 128, 314, 707, 735, 739
GluI GCNGC 5 cut(s) 6, 48, 409, 506, 639
HaeIII GGCC 6 cut(s) 141, 225, 423, 472, 605, 638
HapII CCGG 8 cut(s) 102, 120, 273, 435, 441, 473, 578, 606
Hin1II CATG 3 cut(s) 68, 329, 730
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 2 cut(s) 569, 690
HpaII CCGG 8 cut(s) 102, 120, 273, 435, 441, 473, 578, 606
HphI GGTGA 5 cut(s) 109, 160, 424, 487, 567
Hpy166II GTNNAC 2 cut(s) 526, 535
Hpy188I TCNGA 2 cut(s) 486, 591
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 2 cut(s) 526, 535
Hpy99I CGWCG 3 cut(s) 47, 625, 628
HpyAV CCTTC 3 cut(s) 61, 163, 265
HpyCH4III ACNGT 3 cut(s) 232, 367, 659
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 80, 635, 659, 681
HpyF3I CTNAG 2 cut(s) 387, 483
Hsp92II CATG 3 cut(s) 68, 329, 730
KpnI GGTACC 1 cut(s) 279
KspI CCGCGG 1 cut(s) 642
Kzo9I GATC 3 cut(s) 413, 437, 486
LguI GCTCTTC 1 cut(s) 674
LmnI GCTCC 2 cut(s) 586, 643
Lsp1109I GCAGC 2 cut(s) 17, 517
LweI GCATC 1 cut(s) 132
MaeI CTAG 5 cut(s) 128, 314, 707, 735, 739
MaeIII GTNAC 4 cut(s) 475, 546, 566, 573
MalI GATC 3 cut(s) 415, 439, 488
MbiI CCGCTC 1 cut(s) 468
MboI GATC 3 cut(s) 413, 437, 486
MboII GAAGA 2 cut(s) 46, 691
MflI RGATCY 2 cut(s) 413, 437
MlsI TGGCCA 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 1 cut(s) 614
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 2 cut(s) 111, 281
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 8 cut(s) 102, 120, 273, 435, 441, 473, 578, 606
MspR9I CCNGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 641
MwoI GCNNNNNNNGC 5 cut(s) 47, 80, 635, 659, 681
NciI CCSGG 1 cut(s) 102
NdeII GATC 3 cut(s) 413, 437, 486
NlaIII CATG 3 cut(s) 68, 329, 730
NlaIV GGNNCC 6 cut(s) 84, 277, 439, 449, 582, 645
NmuCI GTSAC 3 cut(s) 475, 566, 573
PciSI GCTCTTC 1 cut(s) 674
PcsI WCGNNNNNNNCGW 1 cut(s) 626
PfeI GAWTC 1 cut(s) 690
PkrI GCNGC 5 cut(s) 7, 49, 410, 507, 640
PleI GAGTC 1 cut(s) 563
PpsI GAGTC 1 cut(s) 563
PspN4I GGNNCC 6 cut(s) 84, 277, 439, 449, 582, 645
PspPI GGNCC 3 cut(s) 223, 470, 603
PsuI RGATCY 2 cut(s) 413, 437
RsaI GTAC 4 cut(s) 277, 323, 341, 527
RsaNI GTAC 4 cut(s) 276, 322, 340, 526
SacII CCGCGG 1 cut(s) 642
SapI GCTCTTC 1 cut(s) 674
SaqAI TTAA 2 cut(s) 111, 281
SatI GCNGC 5 cut(s) 6, 48, 409, 506, 639
Sau3AI GATC 3 cut(s) 413, 437, 486
Sau96I GGNCC 3 cut(s) 223, 470, 603
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 1 cut(s) 102
SfaNI GCATC 1 cut(s) 132
Sfr303I CCGCGG 1 cut(s) 642
SgrBI CCGCGG 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
SspMI CTAG 5 cut(s) 128, 314, 707, 735, 739
StyD4I CCNGG 1 cut(s) 100
TaaI ACNGT 3 cut(s) 232, 367, 659
TaqI TCGA 3 cut(s) 371, 464, 671
TatI WGTACW 1 cut(s) 339
TauI GCSGC 3 cut(s) 50, 411, 641
TfiI GAWTC 1 cut(s) 690
Tru1I TTAA 2 cut(s) 111, 281
Tru9I TTAA 2 cut(s) 111, 281
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 3 cut(s) 475, 566, 573
TseI GCWGC 2 cut(s) 5, 505
Tsp45I GTSAC 3 cut(s) 475, 566, 573
TspDTI ATGAA 1 cut(s) 715
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 1 cut(s) 664
XspI CTAG 5 cut(s) 128, 314, 707, 735, 739
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.