Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0010671

Physical Location & Seq

Genomic Coordinates Reverse (-)

81056614 .. 81059691

Gene Structure 3078 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G053250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGGATTCTGAAGCCGTGGCGTCTTCATTGAGCATTGGCATCAAACACCATCTCTCTCTCCAATCTAATAATGCATCCTCTAGTCCCAGGGCCCCAACTAAGCAATCCTTTTTCATTGGTGTTGCTGGAGGAACTGCATCTGGAAAAACTACAGTGTGCAATTTGATCATCTCCCAGCTCCGTGATCAGCGTGTTGTTTTAGTAAATCAAGATTCATTTTATCACTCCTTGAATGAGGAGAAGTTGAAGACGGTGCATGAATACAACTTTGACCATCCTGAGTCCTTTGATACGGAGTTGTTGCTTTCTTGTATGGAGAAATTAAAGCTTGGACAGGCAGTTAGCATCCCAAATTATGATTTTAAGACCCATCAAAGCATAGAACCCGCTCGCCAGGTTAACCCCTCAGACATTATCATTTTAGAAGGAATACTTGTTCTCCATGATCCTCGTGTCCGTGATCTCATGAACATGAAAATCTTCGTCGACACAGACTCTGATGTACGGCTCTCTAGGAGAATTCAACGTGATACTGTAGAAAGAGGCAGAAATATACAGAATGTGCTTGAACAATATACCAAATTCGTGAAGCCTAGTTTTGATGAATTCATATTGCCCTCGAAAAAGTATGCAGATATAATCATTCCTCGAGTGCAACATATTCATACAAAGCTTGGCCAACATGATCTGTGTAAAATATATCCAAATCTTTTCCTTATCTTTTCCACATTTCAGATACGAGGAATGCATACATTAATCCGTGATGCCCAAACAACAAAGCATGACTTTGTCTTTTACTCAGACCGACTTATTCGATTGGTTGTGGAGCATGGTCTAGGTCATCTTCCCTTCCAAGAACAACAGATTATTACCCCAACAGGATCTATATACAGTGGAGTTGTTTTCTGTAAAAGGTTGTGTGGTGTTTCTGTTATTAGAAGTGGAGAAAGCATGGAGAATGCGCTCAGAGCATGTTGTAAGGGAATAAAAATCGGAAAAATCCTCATCCACGGAGAGGGTAACAGTGGTCGACAGTTAATCTATGAGAAGTTACCGAAAGACATCTCAAGCCGCCATGTCTTATTCCTCGATCCTGTTCTGTCTTCAGGAAACTCTGCCATCAAAGCTATATCTCTGCTACTTAGTAAGGGTGTACCAGAATCCAACATCATCTTCCTTAACCTTATAGCAGCACCGGAGGGAATTCATGCGGTTTTGAAGAGATTTCCAATGCTAAAACTTGTTACTTCAGAGATTGACTTATCTTTGAATGAAGATTTGCGTGTGATCCCAGGAATGGGAGAGTTTGGAGATCGTTATTTTGGTACTGATAGTAATGAGACCGGTTCTGGTTCAAGTTCGAGATCAAGCTCAAAGACACCTAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004849 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006206 GO:0006213 GO:0006220 GO:0006221 GO:0006222 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008655 GO:0009058 GO:0009112 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009173 GO:0009174 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0034404 GO:0034641 GO:0034654 GO:0042455 GO:0043094 GO:0043097 GO:0043174 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046049 GO:0046131 GO:0046132 GO:0046134 GO:0046390 GO:0046483 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00240 ko00983 ko01100 map00240 map00983 map01100

Pfam Domains

Protein Families

PRK UPRTase

Protein Analysis

462

Amino Acids

51.63

Weight (kDa)

8.69

Isoelectric Point (pI)

44.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRK	PF00485	38 - 222	1.3e-49	Phosphoribulokinase / Uridine kinase family
UPRTase	PF14681	242 - 443	3.8e-71	Uracil phosphoribosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010671)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27440 AT3G27440 AT3G27440
fragaria_vesca	FvH4_6g51940
malus_domestica	MD09G1021600.v1.1
prunus_persica	Prupe.3G294700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0173421
rosa_laevigata	RLG00000022188
rosa_multiflora	Rmu_co8519037.1_g000001 Rmu_ssc0000282.1_g000014
rosa_roxburghii	Rroxscaffold_2G00078870
rosa_rugosa	Rorug02G0568200 Rorug02G0568300 Rorug02G0568400 Rorug02G0568500
rosa_samantha	Rh2AG648700 Rh2BG659300 Rh2CG624600 Rh2DG674500
rosa_wichuraiana	Rw2G053250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	389
AccI	GTMKAC	2 cut(s)	486, 1030
AciI	CCGC	3 cut(s)	387, 1072, 1211
AclWI	GGATC	4 cut(s)	440, 889, 1085, 1282
AcoI	YGGCCR	1 cut(s)	676
AcsI	RAATTY	4 cut(s)	519, 581, 605, 1203
AcuI	CTGAAG	3 cut(s)	30, 1089, 1233
AcyI	GRCGYC	1 cut(s)	20
AfaI	GTAC	3 cut(s)	504, 1155, 1327
AfiI	CCNNNNNNNGG	3 cut(s)	1015, 1297, 1298
AgeI	ACCGGT	1 cut(s)	1343
AgsI	TTSAA	7 cut(s)	232, 247, 524, 569, 1219, 1270, 1356
AjnI	CCWGG	3 cut(s)	86, 393, 1291
AjuI	GAANNNNNNNTTGG	2 cut(s)	1157, 1189
AloI	GAACNNNNNNTCC	2 cut(s)	420, 452
AluBI	AGCT	5 cut(s)	178, 328, 673, 1127, 1371
AluI	AGCT	5 cut(s)	178, 328, 673, 1127, 1371
Alw26I	GTCTC	1 cut(s)	1334
AlwI	GGATC	4 cut(s)	440, 889, 1085, 1282
AlwNI	CAGNNNCTG	1 cut(s)	497
Ama87I	CYCGRG	1 cut(s)	648
AoxI	GGCC	2 cut(s)	90, 676
ApaI	GGGCCC	1 cut(s)	94
ApeKI	GCWGC	1 cut(s)	1190
ApoI	RAATTY	4 cut(s)	519, 581, 605, 1203
AseI	ATTAAT	1 cut(s)	755
AsiGI	ACCGGT	1 cut(s)	1343
Asp700I	GAANNNNTTC	1 cut(s)	479
AspLEI	GCGC	1 cut(s)	964
AspS9I	GGNCC	2 cut(s)	90, 91
AvaI	CYCGRG	1 cut(s)	648
BaeGI	GKGCMC	1 cut(s)	94
BalI	TGGCCA	1 cut(s)	678
BanII	GRGCYC	1 cut(s)	94
BauI	CACGAG	1 cut(s)	450
BbsI	GAAGAC	3 cut(s)	15, 254, 1095
BbvI	GCAGC	1 cut(s)	1202
BccI	CCATC	4 cut(s)	57, 282, 378, 1127
BceAI	ACGGC	1 cut(s)	521
BciT130I	CCWGG	3 cut(s)	88, 395, 1293
BclI	TGATCA	2 cut(s)	165, 184
BcoDI	GTCTC	1 cut(s)	1334
BfaI	CTAG	4 cut(s)	81, 513, 594, 836
BfmI	CTRYAG	2 cut(s)	150, 534
BisI	GCNGC	2 cut(s)	1072, 1191
BlsI	GCNGC	2 cut(s)	1073, 1192
Bme1390I	CCNGG	3 cut(s)	88, 395, 1293
BmeT110I	CYCGRG	1 cut(s)	648
BmgT120I	GGNCC	2 cut(s)	90, 91
BmiI	GGNNCC	2 cut(s)	92, 93
BmrFI	CCNGG	3 cut(s)	88, 395, 1293
BmsI	GCATC	5 cut(s)	48, 83, 146, 354, 754
BpiI	GAAGAC	3 cut(s)	15, 254, 1095
BpmI	CTGGAG	1 cut(s)	147
BpuEI	CTTGAG	1 cut(s)	1051
BsaHI	GRCGYC	1 cut(s)	20
BsaI	GGTCTC	1 cut(s)	1334
BsaJI	CCNNGG	5 cut(s)	15, 86, 87, 1009, 1291
BsaWI	WCCGGW	2 cut(s)	1195, 1343
BsaXI	ACNNNNNCTCC	2 cut(s)	423, 453
Bsc4I	CCNNNNNNNGG	3 cut(s)	1015, 1297, 1298
Bse118I	RCCGGY	1 cut(s)	1343
BseBI	CCWGG	3 cut(s)	88, 395, 1293
BseDI	CCNNGG	5 cut(s)	15, 86, 87, 1009, 1291
BseGI	GGATG	4 cut(s)	74, 274, 345, 1005
BseLI	CCNNNNNNNGG	3 cut(s)	1015, 1297, 1298
BseMII	CTCAG	4 cut(s)	270, 420, 813, 979
BseRI	GAGGAG	1 cut(s)	251
BseSI	GKGCMC	1 cut(s)	94
BseXI	GCAGC	1 cut(s)	1202
BseYI	CCCAGC	1 cut(s)	174
BshFI	GGCC	2 cut(s)	92, 678
BshTI	ACCGGT	1 cut(s)	1343
BsiHKCI	CYCGRG	1 cut(s)	648
BsiSI	CCGG	2 cut(s)	1196, 1344
BslFI	GGGAC	1 cut(s)	69
BslI	CCNNNNNNNGG	3 cut(s)	1015, 1297, 1298
BsmAI	GTCTC	1 cut(s)	1334
BsmFI	GGGAC	1 cut(s)	69
BsmI	GAATGC	2 cut(s)	750, 964
BsnI	GGCC	2 cut(s)	92, 678
Bso31I	GGTCTC	1 cut(s)	1334
BsoBI	CYCGRG	1 cut(s)	648
Bsp120I	GGGCCC	1 cut(s)	90
Bsp1286I	GDGCHC	1 cut(s)	94
BspACI	CCGC	3 cut(s)	387, 1072, 1211
BspANI	GGCC	2 cut(s)	92, 678
BspCNI	CTCAG	4 cut(s)	271, 419, 812, 978
BspHI	TCATGA	1 cut(s)	465
BspLI	GGNNCC	2 cut(s)	92, 93
BspPI	GGATC	4 cut(s)	440, 889, 1085, 1282
BspTNI	GGTCTC	1 cut(s)	1334
BsrBI	CCGCTC	1 cut(s)	389
BsrFI	RCCGGY	1 cut(s)	1343
BssAI	RCCGGY	1 cut(s)	1343
BssECI	CCNNGG	5 cut(s)	15, 86, 87, 1009, 1291
BssNI	GRCGYC	1 cut(s)	20
BssSI	CACGAG	1 cut(s)	450
Bst2BI	CACGAG	1 cut(s)	450
Bst2UI	CCWGG	3 cut(s)	88, 395, 1293
Bst4CI	ACNGT	6 cut(s)	154, 253, 535, 893, 1025, 1035
Bst6I	CTCTTC	1 cut(s)	1214
BstACI	GRCGYC	1 cut(s)	20
BstC8I	GCNNGC	1 cut(s)	391
BstDEI	CTNAG	6 cut(s)	99, 279, 406, 799, 965, 1142
BstDSI	CCRYGG	2 cut(s)	15, 1009
BstF5I	GGATG	4 cut(s)	74, 274, 345, 1005
BstHHI	GCGC	1 cut(s)	964
BstMAI	GTCTC	1 cut(s)	1334
BstMWI	GCNNNNNNNGC	2 cut(s)	968, 1124
BstNI	CCWGG	3 cut(s)	88, 395, 1293
BstNSI	RCATGY	1 cut(s)	975
BstSCI	CCNGG	3 cut(s)	86, 393, 1291
BstSFI	CTRYAG	2 cut(s)	150, 534
BstSLI	GKGCMC	1 cut(s)	94
BstV1I	GCAGC	1 cut(s)	1202
BstV2I	GAAGAC	3 cut(s)	15, 254, 1095
BstX2I	RGATCY	1 cut(s)	881
BstYI	RGATCY	1 cut(s)	881
BsuRI	GGCC	2 cut(s)	92, 678
BtgI	CCRYGG	2 cut(s)	15, 1009
BtsCI	GGATG	4 cut(s)	74, 274, 345, 1005
BtsIMutI	CAGTG	3 cut(s)	159, 898, 1030
Cac8I	GCNNGC	1 cut(s)	391
CaiI	CAGNNNCTG	1 cut(s)	497
CciI	TCATGA	1 cut(s)	465
CfoI	GCGC	1 cut(s)	964
Cfr10I	RCCGGY	1 cut(s)	1343
Cfr13I	GGNCC	2 cut(s)	90, 91
CseI	GACGC	1 cut(s)	9
Csp6I	GTAC	3 cut(s)	503, 1154, 1326
CspAI	ACCGGT	1 cut(s)	1343
CviQI	GTAC	3 cut(s)	503, 1154, 1326
DdeI	CTNAG	6 cut(s)	99, 279, 406, 799, 965, 1142
EaeI	YGGCCR	1 cut(s)	676
Eam1104I	CTCTTC	1 cut(s)	1214
EarI	CTCTTC	1 cut(s)	1214
Eco24I	GRGCYC	1 cut(s)	94
Eco31I	GGTCTC	1 cut(s)	1334
Eco57I	CTGAAG	3 cut(s)	30, 1089, 1233
Eco88I	CYCGRG	1 cut(s)	648
EcoO109I	RGGNCCY	2 cut(s)	90, 91
EcoRI	GAATTC	3 cut(s)	519, 605, 1203
EcoRII	CCWGG	3 cut(s)	86, 393, 1291
EcoT22I	ATGCAT	2 cut(s)	76, 750
EcoT38I	GRGCYC	1 cut(s)	94
FalI	AAGNNNNNCTT	6 cut(s)	92, 124, 417, 449, 770, 802
FaqI	GGGAC	1 cut(s)	69
FauI	CCCGC	1 cut(s)	394
FbaI	TGATCA	2 cut(s)	165, 184
FblI	GTMKAC	2 cut(s)	486, 1030
Fnu4HI	GCNGC	2 cut(s)	1072, 1191
FokI	GGATG	4 cut(s)	61, 261, 332, 992
FriOI	GRGCYC	1 cut(s)	94
Fsp4HI	GCNGC	2 cut(s)	1072, 1191
FspBI	CTAG	4 cut(s)	81, 513, 594, 836
GlaI	GCGC	1 cut(s)	963
GluI	GCNGC	2 cut(s)	1072, 1191
GsaI	CCCAGC	1 cut(s)	178
GsuI	CTGGAG	1 cut(s)	147
HaeIII	GGCC	2 cut(s)	92, 678
HapII	CCGG	2 cut(s)	1196, 1344
HgaI	GACGC	1 cut(s)	9
HhaI	GCGC	1 cut(s)	964
Hin1I	GRCGYC	1 cut(s)	20
Hin6I	GCGC	1 cut(s)	962
HinP1I	GCGC	1 cut(s)	962
HincII	GTYRAC	3 cut(s)	400, 487, 1031
HindII	GTYRAC	3 cut(s)	400, 487, 1031
HindIII	AAGCTT	2 cut(s)	326, 671
HinfI	GANTC	5 cut(s)	5, 212, 281, 494, 1160
HpaI	GTTAAC	1 cut(s)	400
HpaII	CCGG	2 cut(s)	1196, 1344
Hpy166II	GTNNAC	4 cut(s)	400, 487, 1031, 1154
Hpy188I	TCNGA	8 cut(s)	10, 409, 499, 735, 802, 968, 995, 1252
Hpy188III	TCNNGA	7 cut(s)	141, 209, 278, 466, 586, 1107, 1362
Hpy8I	GTNNAC	4 cut(s)	400, 487, 1031, 1154
Hpy99I	CGWCG	1 cut(s)	488
HpyAV	CCTTC	2 cut(s)	419, 859
HpyCH4III	ACNGT	6 cut(s)	154, 253, 535, 893, 1025, 1035
HpyCH4IV	ACGT	1 cut(s)	526
HpyCH4V	TGCA	7 cut(s)	74, 137, 159, 256, 632, 655, 748
HpyF10VI	GCNNNNNNNGC	2 cut(s)	968, 1124
HpyF3I	CTNAG	6 cut(s)	99, 279, 406, 799, 965, 1142
HpySE526I	ACGT	1 cut(s)	526
Hsp92I	GRCGYC	1 cut(s)	20
HspAI	GCGC	1 cut(s)	962
Ksp22I	TGATCA	2 cut(s)	165, 184
KspAI	GTTAAC	1 cut(s)	400
LmnI	GCTCC	2 cut(s)	183, 826
Lsp1109I	GCAGC	1 cut(s)	1202
LweI	GCATC	5 cut(s)	48, 83, 146, 354, 754
MaeI	CTAG	4 cut(s)	81, 513, 594, 836
MaeII	ACGT	1 cut(s)	526
MaeIII	GTNAC	3 cut(s)	1019, 1050, 1243
MbiI	CCGCTC	1 cut(s)	389
MboII	GAAGA	8 cut(s)	15, 259, 472, 836, 1095, 1165, 1231, 1286
MflI	RGATCY	1 cut(s)	881
MhlI	GDGCHC	1 cut(s)	94
MlsI	TGGCCA	1 cut(s)	678
MluCI	AATT	7 cut(s)	160, 320, 352, 519, 581, 605, 1203
MluNI	TGGCCA	1 cut(s)	678
MlyI	GAGTC	2 cut(s)	290, 488
MmeI	TCCRAC	1 cut(s)	1188
Mox20I	TGGCCA	1 cut(s)	678
Mph1103I	ATGCAT	2 cut(s)	76, 750
MroXI	GAANNNNTTC	1 cut(s)	479
MscI	TGGCCA	1 cut(s)	678
MseI	TTAA	6 cut(s)	323, 363, 399, 755, 1037, 1179
MslI	CAYNNNNRTG	1 cut(s)	470
Msp20I	TGGCCA	1 cut(s)	678
MspI	CCGG	2 cut(s)	1196, 1344
MspR9I	CCNGG	3 cut(s)	88, 395, 1293
Mva1269I	GAATGC	2 cut(s)	750, 964
MvaI	CCWGG	3 cut(s)	88, 395, 1293
MwoI	GCNNNNNNNGC	2 cut(s)	968, 1124
NlaIV	GGNNCC	2 cut(s)	92, 93
NsiI	ATGCAT	2 cut(s)	76, 750
NspI	RCATGY	1 cut(s)	975
PaeR7I	CTCGAG	1 cut(s)	648
PagI	TCATGA	1 cut(s)	465
PasI	CCCWGGG	1 cut(s)	87
PctI	GAATGC	2 cut(s)	750, 964
PdmI	GAANNNNTTC	1 cut(s)	479
PfeI	GAWTC	3 cut(s)	5, 212, 1160
PflFI	GACNNNGTC	1 cut(s)	788
PinAI	ACCGGT	1 cut(s)	1343
PkrI	GCNGC	2 cut(s)	1073, 1192
PleI	GAGTC	2 cut(s)	289, 488
PpsI	GAGTC	2 cut(s)	289, 488
PshBI	ATTAAT	1 cut(s)	755
Psp6I	CCWGG	3 cut(s)	86, 393, 1291
PspFI	CCCAGC	1 cut(s)	174
PspGI	CCWGG	3 cut(s)	86, 393, 1291
PspN4I	GGNNCC	2 cut(s)	92, 93
PspOMI	GGGCCC	1 cut(s)	90
PspPI	GGNCC	2 cut(s)	90, 91
PspXI	VCTCGAGB	1 cut(s)	648
PstNI	CAGNNNCTG	1 cut(s)	497
PsuI	RGATCY	1 cut(s)	881
PsyI	GACNNNGTC	1 cut(s)	788
RsaI	GTAC	3 cut(s)	504, 1155, 1327
RsaNI	GTAC	3 cut(s)	503, 1154, 1326
RseI	CAYNNNNRTG	1 cut(s)	470
SalI	GTCGAC	2 cut(s)	485, 1029
SaqAI	TTAA	6 cut(s)	323, 363, 399, 755, 1037, 1179
SatI	GCNGC	2 cut(s)	1072, 1191
Sau96I	GGNCC	2 cut(s)	90, 91
SchI	GAGTC	2 cut(s)	290, 488
ScrFI	CCNGG	3 cut(s)	88, 395, 1293
SduI	GDGCHC	1 cut(s)	94
SfaNI	GCATC	5 cut(s)	48, 83, 146, 354, 754
SfcI	CTRYAG	2 cut(s)	150, 534
Sfr274I	CTCGAG	1 cut(s)	648
SlaI	CTCGAG	1 cut(s)	648
SmiMI	CAYNNNNRTG	1 cut(s)	470
SmlI	CTYRAG	2 cut(s)	648, 1066
SmoI	CTYRAG	2 cut(s)	648, 1066
Sse9I	AATT	7 cut(s)	160, 320, 352, 519, 581, 605, 1203
SsiI	CCGC	3 cut(s)	387, 1072, 1211
SspMI	CTAG	4 cut(s)	81, 513, 594, 836
StyD4I	CCNGG	3 cut(s)	86, 393, 1291
TaaI	ACNGT	6 cut(s)	154, 253, 535, 893, 1025, 1035
TaiI	ACGT	1 cut(s)	529
TaqI	TCGA	7 cut(s)	486, 620, 649, 814, 1030, 1089, 1361
TaqII	GACCGA	1 cut(s)	819
TasI	AATT	7 cut(s)	160, 320, 352, 519, 581, 605, 1203
TauI	GCSGC	1 cut(s)	1074
TfiI	GAWTC	3 cut(s)	5, 212, 1160
Tru1I	TTAA	6 cut(s)	323, 363, 399, 755, 1037, 1179
Tru9I	TTAA	6 cut(s)	323, 363, 399, 755, 1037, 1179
TscAI	CASTG	3 cut(s)	159, 898, 1030
TseI	GCWGC	1 cut(s)	1190
TspGWI	ACGGA	5 cut(s)	170, 308, 446, 749, 1026
TspRI	CASTG	3 cut(s)	159, 898, 1030
Tth111I	GACNNNGTC	1 cut(s)	788
VspI	ATTAAT	1 cut(s)	755
XapI	RAATTY	4 cut(s)	519, 581, 605, 1203
XceI	RCATGY	1 cut(s)	975
XhoI	CTCGAG	1 cut(s)	648
XmiI	GTMKAC	2 cut(s)	486, 1030
XmnI	GAANNNNTTC	1 cut(s)	479
XspI	CTAG	4 cut(s)	81, 513, 594, 836
Zsp2I	ATGCAT	2 cut(s)	76, 750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.