Rw5G011990

Gamma-soluble nsf attachment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008536
Physical Location & Seq
Reverse (-)
13956430 .. 13960457
4028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011990.1

Sequence Viewer

Length: 810 bp
ATGCTGATTGGAAAAGCACTACTCTTCTGTATGAACAAGCGGGTACCTAATGGGTTTAGGATTGCCCAGGATGATGAGAAAGCAAAGGACGCGCTGGAAAAAGCATTAAAAGGACAAGAACCCTGGGATGCTGCAAAGCATATGGAATCTGCTGCTGCTCTAGCAAAGGAGTTAGGCAAGTGGAATGAAGTTGCTGATTTTTACACAAAGGCATCGGAATTATACACTGTTTGCGGAAGAGCACAGCCTGTATCAGAGGCTCTTGCAAAAGGAGCCCGTGCTCTTGAAGTTCCTGTGCCCGAAGAAGCTATTCAACTTTACATTGAGGCGTGCACTATTCTTGAAGACGATGACAAAGAGCAAATGGCCTTTGATCTATATCGAGATGTTACTAGTGCGTATATAAAACTACCGAAGTATACAAATGCTGTGGCTTTCCTGTTGAGATGGGGCTTAGCAGCTGATAAGTGCAATGCTACCCATAGCCAGTGCAAGGCATATCTTAGTGCAATTCTCATAGACCTGTATATTCATGACGTCAAGCAGGCAGAAAAATGCTACAACGATTGCTCCCAGGTCGATGCTTTTTTGGGCAATGATCATGGCCAATGTGCTAGTAAACTTCTTTCCGCTTACACGGAAGGTGATATTGAAGAAATCAAACGACTTGCTCAGTCAAGCGCAGTTTCTCATCTTGATCACATGGTCATCAGACTCGCCAGGAAACTGCCTACAGGTGATGTCAGTGGACTAAAGACAAATGTGTCTAAGGAGCAAGAAGAAGAATTGGATGAAGATGACCTTACATAG

Protein Analysis

269

Amino Acids

29.87

Weight (kDa)

4.98

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNAP PF14938 17 - 237 4.3e-13 Soluble NSF attachment protein, SNAP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 763
AatII GACGTC 1 cut(s) 540
Acc65I GGTACC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 419
AccII CGCG 1 cut(s) 92
AciI CCGC 3 cut(s) 40, 234, 630
AcoI YGGCCR 1 cut(s) 604
AcyI GRCGYC 1 cut(s) 537
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 493
AgsI TTSAA 4 cut(s) 287, 314, 344, 653
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 4 cut(s) 66, 122, 573, 719
AluBI AGCT 2 cut(s) 308, 461
AluI AGCT 2 cut(s) 308, 461
Alw21I GWGCWC 3 cut(s) 244, 283, 335
Alw44I GTGCAC 1 cut(s) 331
AoxI GGCC 2 cut(s) 366, 604
ApaLI GTGCAC 1 cut(s) 331
ApeKI GCWGC 4 cut(s) 131, 152, 155, 458
Asp700I GAANNNNTTC 1 cut(s) 309
Asp718I GGTACC 1 cut(s) 43
AspLEI GCGC 2 cut(s) 94, 683
AsuHPI GGTGA 2 cut(s) 656, 749
BaeGI GKGCMC 2 cut(s) 300, 335
BalI TGGCCA 1 cut(s) 606
BanI GGYRCC 1 cut(s) 43
BanII GRGCYC 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 3 cut(s) 244, 283, 335
BbvI GCAGC 4 cut(s) 118, 139, 142, 470
BccI CCATC 1 cut(s) 441
BciT130I CCWGG 4 cut(s) 68, 124, 575, 721
BclI TGATCA 2 cut(s) 598, 697
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 3 cut(s) 161, 393, 615
BfmI CTRYAG 1 cut(s) 732
BisI GCNGC 4 cut(s) 132, 153, 156, 459
BlpI GCTNAGC 1 cut(s) 454
BlsI GCNGC 4 cut(s) 133, 154, 157, 460
Bme1390I CCNGG 4 cut(s) 68, 124, 575, 721
BmiI GGNNCC 2 cut(s) 45, 274
BmrFI CCNGG 4 cut(s) 68, 124, 575, 721
BmsI GCATC 3 cut(s) 118, 221, 571
BpiI GAAGAC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 454
BsaBI GATNNNNATC 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 4 cut(s) 66, 122, 123, 573
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 2 cut(s) 478, 601
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 4 cut(s) 68, 124, 575, 721
BseDI CCNNGG 4 cut(s) 66, 122, 123, 573
BseGI GGATG 3 cut(s) 76, 133, 796
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMI GCAATG 2 cut(s) 478, 601
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 1 cut(s) 487
BseSI GKGCMC 2 cut(s) 300, 335
BseXI GCAGC 4 cut(s) 118, 139, 142, 470
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 2 cut(s) 368, 606
BshNI GGYRCC 1 cut(s) 43
BsiHKAI GWGCWC 3 cut(s) 244, 283, 335
BslI CCNNNNNNNGG 1 cut(s) 493
BsnI GGCC 2 cut(s) 368, 606
Bsp1286I GDGCHC 5 cut(s) 244, 277, 283, 300, 335
Bsp143I GATC 3 cut(s) 373, 598, 697
Bsp1720I GCTNAGC 1 cut(s) 454
BspACI CCGC 3 cut(s) 40, 234, 630
BspANI GGCC 2 cut(s) 368, 606
BspCNI CTCAG 1 cut(s) 685
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 2 cut(s) 45, 274
BspQI GCTCTTC 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 43
BsrDI GCAATG 2 cut(s) 478, 601
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 4 cut(s) 66, 122, 123, 573
BssMI GATC 3 cut(s) 373, 598, 697
BssNAI GTATAC 1 cut(s) 420
BssNI GRCGYC 1 cut(s) 537
Bst1107I GTATAC 1 cut(s) 420
Bst2UI CCWGG 4 cut(s) 68, 124, 575, 721
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 2 cut(s) 29, 232
BstACI GRCGYC 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 331, 546
BstDEI CTNAG 4 cut(s) 454, 503, 672, 768
BstF5I GGATG 3 cut(s) 76, 133, 796
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 2 cut(s) 94, 683
BstKTI GATC 3 cut(s) 376, 601, 700
BstMBI GATC 3 cut(s) 373, 598, 697
BstMWI GCNNNNNNNGC 3 cut(s) 89, 161, 272
BstNI CCWGG 4 cut(s) 68, 124, 575, 721
BstSCI CCNGG 4 cut(s) 66, 122, 573, 719
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 2 cut(s) 300, 335
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 4 cut(s) 118, 139, 142, 470
BstV2I GAAGAC 1 cut(s) 351
BstZ17I GTATAC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 368, 606
BtsCI GGATG 3 cut(s) 76, 133, 796
BtsIMutI CAGTG 3 cut(s) 225, 494, 751
Cac8I GCNNGC 2 cut(s) 331, 546
CciI TCATGA 1 cut(s) 532
CfoI GCGC 2 cut(s) 94, 683
CseI GACGC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 44
CspCI CAANNNNNGTGG 2 cut(s) 411, 446
CviAII CATG 3 cut(s) 533, 602, 703
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 4 cut(s) 454, 503, 672, 768
DpnI GATC 3 cut(s) 375, 600, 699
DpnII GATC 3 cut(s) 373, 598, 697
DrdI GACNNNNNNGTC 1 cut(s) 763
DseDI GACNNNNNNGTC 1 cut(s) 763
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 2 cut(s) 29, 232
EarI CTCTTC 2 cut(s) 29, 232
Eco24I GRGCYC 1 cut(s) 277
EcoRII CCWGG 4 cut(s) 66, 122, 573, 719
EcoT38I GRGCYC 1 cut(s) 277
FaeI CATG 3 cut(s) 536, 605, 706
FalI AAGNNNNNCTT 1 cut(s) 786
FatI CATG 3 cut(s) 532, 601, 702
FauI CCCGC 1 cut(s) 33
FauNDI CATATG 1 cut(s) 141
FbaI TGATCA 2 cut(s) 598, 697
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 4 cut(s) 132, 153, 156, 459
FokI GGATG 3 cut(s) 83, 140, 803
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 4 cut(s) 132, 153, 156, 459
FspBI CTAG 3 cut(s) 161, 393, 615
GlaI GCGC 2 cut(s) 93, 682
GluI GCNGC 4 cut(s) 132, 153, 156, 459
HaeIII GGCC 2 cut(s) 368, 606
HgaI GACGC 1 cut(s) 98
HhaI GCGC 2 cut(s) 94, 683
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 3 cut(s) 536, 605, 706
Hin6I GCGC 2 cut(s) 92, 681
HinP1I GCGC 2 cut(s) 92, 681
HinfI GANTC 2 cut(s) 146, 714
HphI GGTGA 2 cut(s) 656, 749
Hpy166II GTNNAC 4 cut(s) 333, 420, 620, 749
Hpy188I TCNGA 3 cut(s) 217, 256, 713
Hpy188III TCNNGA 5 cut(s) 284, 341, 383, 533, 695
Hpy8I GTNNAC 4 cut(s) 333, 420, 620, 749
HpyAV CCTTC 1 cut(s) 635
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 537
HpyCH4V TGCA 6 cut(s) 134, 266, 333, 471, 492, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 161, 272
HpyF3I CTNAG 4 cut(s) 454, 503, 672, 768
HpySE526I ACGT 1 cut(s) 537
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 3 cut(s) 536, 605, 706
HspAI GCGC 2 cut(s) 92, 681
KpnI GGTACC 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 598, 697
Kzo9I GATC 3 cut(s) 373, 598, 697
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 3 cut(s) 272, 575, 772
Lsp1109I GCAGC 4 cut(s) 118, 139, 142, 470
LweI GCATC 3 cut(s) 118, 221, 571
MaeI CTAG 3 cut(s) 161, 393, 615
MaeII ACGT 1 cut(s) 537
MaeIII GTNAC 1 cut(s) 388
MalI GATC 3 cut(s) 375, 600, 699
MboI GATC 3 cut(s) 373, 598, 697
MboII GAAGA 8 cut(s) 16, 249, 314, 356, 665, 791, 794, 806
MhlI GDGCHC 5 cut(s) 244, 277, 283, 300, 335
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 3 cut(s) 218, 510, 785
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 1 cut(s) 708
MnlI CCTC 2 cut(s) 250, 319
Mox20I TGGCCA 1 cut(s) 606
MroXI GAANNNNTTC 1 cut(s) 309
MscI TGGCCA 1 cut(s) 606
MseI TTAA 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 606
MspA1I CMGCKG 1 cut(s) 461
MspR9I CCNGG 4 cut(s) 68, 124, 575, 721
MvaI CCWGG 4 cut(s) 68, 124, 575, 721
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 89, 161, 272
NdeI CATATG 1 cut(s) 141
NdeII GATC 3 cut(s) 373, 598, 697
NlaIII CATG 3 cut(s) 536, 605, 706
NlaIV GGNNCC 2 cut(s) 45, 274
PagI TCATGA 1 cut(s) 532
PasI CCCWGGG 1 cut(s) 123
PciSI GCTCTTC 1 cut(s) 232
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 1 cut(s) 146
PkrI GCNGC 4 cut(s) 133, 154, 157, 460
PleI GAGTC 1 cut(s) 708
PpsI GAGTC 1 cut(s) 708
Psp6I CCWGG 4 cut(s) 66, 122, 573, 719
PspGI CCWGG 4 cut(s) 66, 122, 573, 719
PspN4I GGNNCC 2 cut(s) 45, 274
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 4 cut(s) 132, 153, 156, 459
Sau3AI GATC 3 cut(s) 373, 598, 697
SchI GAGTC 1 cut(s) 708
ScrFI CCNGG 4 cut(s) 68, 124, 575, 721
SduI GDGCHC 5 cut(s) 244, 277, 283, 300, 335
SetI ASST 9 cut(s) 49, 310, 463, 525, 540, 579, 646, 739, 804
SfaNI GCATC 3 cut(s) 118, 221, 571
SfcI CTRYAG 1 cut(s) 732
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 3 cut(s) 218, 510, 785
SsiI CCGC 3 cut(s) 40, 234, 630
SspMI CTAG 3 cut(s) 161, 393, 615
StyD4I CCNGG 4 cut(s) 66, 122, 573, 719
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 540
TaqI TCGA 2 cut(s) 382, 579
TasI AATT 3 cut(s) 218, 510, 785
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TscAI CASTG 3 cut(s) 232, 494, 751
TseI GCWGC 4 cut(s) 131, 152, 155, 458
TspDTI ATGAA 4 cut(s) 47, 201, 521, 807
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 3 cut(s) 232, 494, 751
VneI GTGCAC 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 3 cut(s) 161, 393, 615
ZraI GACGTC 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.