Rw6G040630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016021
Physical Location & Seq
Reverse (-)
64415273 .. 64416168
896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040630.1

Sequence Viewer

Length: 858 bp
ATGGGTTCTTGCATAAGCAAATGCAGTCCCAAAAACCACTTCCAAGTCGAAGAAGATGATCAATTCTGCAATCATGTCCAAGACAAACTGGTAATCTCGACCCAACCTCCTAAACCTACCACTCCAATTCCTTCTCACCATTCAAACAAAATCTCACCCTTTCCTTCTTCTCCTTCAAATTCCACTTCCTCAGCTTCTTCCTTCAGTTATTGCCCCACCACCACTACTAGTAGTACTACTAATTCTAACTCCACCATCAGCTCATCGAGCTCAACCTTGTCAAGCAGCGCTTCTTCAGTTTCGAGCAGTTCAAAGATCGACAGATCATTTTCGAACGAGTTCTTGTGGTCTTGCTACAAAGAAAACCAGCATATATCTCGCATTGCTTCAATCAAGGAAGCACAAAAACCAGTTGCAGCGGCAACGTTGAAAAAACAGCAGCAGCCGAGCCCAATAAACAGAGGAAATGGGTGTACTAAAACGACGCCGCAGAAGAGAGTCCGGACAAGTACTTCACCAACTCTGAAGCGACAGAAGAGTTTCCGTAAAGAGCCCGAGAAGCCCGTGATCTCTGCATATTCTAGAAGCTTGAGGTCACCGTCTCCGAGTAGAAGGTTCAATGTTTCAGAGAAGAACAGGGAACAGCCGGCTAGTCCGAACAACAACCCAAGTGGGTTAATGGCTCGGCCTTGTTTGAAGAGTCCTGCGAGAGAGACACAGACAAGGATTCACAGGATCAGTTCCAAAATCGATGAAGTTGCTGTTAGAGAAGCTCTGGCTCATGACCATTACATGGAGTCTGTTCCTGAGGACATTGATAACCCTCTAATCTCTTTGGATTGTTTTATTTTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

31.4

Weight (kDa)

9.58

Isoelectric Point (pI)

73.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21510
fragaria_vesca FvH4_2g35040
malus_domestica MD15G1088100.v1.1
prunus_persica Prupe.1G442100_v2.0.a1
pyrus_communis pycom08g08960
rosa_chinensis RchiOBHm_Chr6g0306951
rosa_laevigata RLG00000010751
rosa_multiflora Rmu_sc0007920.1_g000035
rosa_roxburghii Rroxscaffold_7G00161330
rosa_rugosa Rorug06G0354700
rosa_samantha Rh6AG466400 Rh6BG434600 Rh6CG481300 Rh6DG467700
rosa_wichuraiana Rw6G040630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 793
AccIII TCCGGA 1 cut(s) 501
AciI CCGC 2 cut(s) 419, 488
AclI AACGTT 1 cut(s) 425
AclWI GGATC 1 cut(s) 743
AcsI RAATTY 1 cut(s) 178
AcuI CTGAAG 3 cut(s) 187, 279, 545
AcyI GRCGYC 1 cut(s) 485
AfaI GTAC 3 cut(s) 235, 475, 511
AfeI AGCGCT 1 cut(s) 289
AfiI CCNNNNNNNGG 1 cut(s) 793
AgsI TTSAA 7 cut(s) 144, 177, 312, 390, 430, 619, 697
AhlI ACTAGT 1 cut(s) 227
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 5 cut(s) 194, 261, 270, 588, 773
AluI AGCT 5 cut(s) 194, 261, 270, 588, 773
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 2 cut(s) 606, 707
AlwI GGATC 1 cut(s) 743
Ama87I CYCGRG 1 cut(s) 554
Aor13HI TCCGGA 1 cut(s) 501
Aor51HI AGCGCT 1 cut(s) 289
AoxI GGCC 1 cut(s) 686
ApeKI GCWGC 4 cut(s) 285, 416, 439, 442
ApoI RAATTY 1 cut(s) 178
Asp700I GAANNNNTTC 2 cut(s) 338, 539
AspLEI GCGC 1 cut(s) 290
AsuHPI GGTGA 4 cut(s) 128, 147, 507, 588
AsuII TTCGAA 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 554
AxyI CCTNAGG 1 cut(s) 807
BanII GRGCYC 3 cut(s) 272, 452, 555
Bbv12I GWGCWC 1 cut(s) 272
BbvCI CCTCAGC 1 cut(s) 190
BbvI GCAGC 4 cut(s) 297, 428, 451, 454
BccI CCATC 1 cut(s) 263
BcgI CGANNNNNNTGC 4 cut(s) 359, 393, 740, 774
BclI TGATCA 1 cut(s) 58
BcoDI GTCTC 2 cut(s) 606, 707
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 3 cut(s) 228, 582, 651
BfoI RGCGCY 1 cut(s) 291
BisI GCNGC 6 cut(s) 286, 417, 420, 440, 443, 488
BlsI GCNGC 6 cut(s) 287, 418, 421, 441, 444, 489
BmcAI AGTACT 2 cut(s) 235, 511
BmeT110I CYCGRG 1 cut(s) 554
Bpu10I CCTNAGC 1 cut(s) 190
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 610
Bsa29I ATCGAT 1 cut(s) 750
BsaHI GRCGYC 1 cut(s) 485
BsaWI WCCGGW 1 cut(s) 501
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 1 cut(s) 793
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 2 cut(s) 93, 410
Bse21I CCTNAGG 1 cut(s) 807
Bse3DI GCAATG 1 cut(s) 381
BseAI TCCGGA 1 cut(s) 501
BseCI ATCGAT 1 cut(s) 750
BseLI CCNNNNNNNGG 1 cut(s) 793
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 204, 798
BseNI ACTGG 2 cut(s) 93, 410
BseXI GCAGC 4 cut(s) 297, 428, 451, 454
BshFI GGCC 1 cut(s) 688
BshVI ATCGAT 1 cut(s) 750
BsiHKAI GWGCWC 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 554
BsiSI CCGG 2 cut(s) 502, 647
BslFI GGGAC 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 793
BsmAI GTCTC 2 cut(s) 606, 707
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 12
BsnI GGCC 1 cut(s) 688
BsoBI CYCGRG 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 3 cut(s) 272, 452, 555
Bsp13I TCCGGA 1 cut(s) 501
Bsp143I GATC 5 cut(s) 58, 315, 323, 567, 735
BspACI CCGC 2 cut(s) 419, 488
BspANI GGCC 1 cut(s) 688
BspCNI CTCAG 2 cut(s) 203, 799
BspDI ATCGAT 1 cut(s) 750
BspEI TCCGGA 1 cut(s) 501
BspHI TCATGA 1 cut(s) 781
BspPI GGATC 1 cut(s) 743
BspT104I TTCGAA 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 2 cut(s) 93, 410
BssAI RCCGGY 1 cut(s) 646
BssMI GATC 5 cut(s) 58, 315, 323, 567, 735
BssNI GRCGYC 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 600
Bst6I CTCTTC 3 cut(s) 488, 530, 692
BstACI GRCGYC 1 cut(s) 485
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 2 cut(s) 190, 807
BstEII GGTNACC 1 cut(s) 594
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 5 cut(s) 61, 318, 326, 570, 738
BstMAI GTCTC 2 cut(s) 606, 707
BstMBI GATC 5 cut(s) 58, 315, 323, 567, 735
BstMWI GCNNNNNNNGC 2 cut(s) 267, 559
BstPI GGTNACC 1 cut(s) 594
BstV1I GCAGC 4 cut(s) 297, 428, 451, 454
Bsu15I ATCGAT 1 cut(s) 750
Bsu36I CCTNAGG 1 cut(s) 807
BsuRI GGCC 1 cut(s) 688
BsuTUI ATCGAT 1 cut(s) 750
Cac8I GCNNGC 1 cut(s) 648
CciI TCATGA 1 cut(s) 781
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 1 cut(s) 646
ClaI ATCGAT 1 cut(s) 750
CseI GACGC 1 cut(s) 493
Csp6I GTAC 3 cut(s) 234, 474, 510
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 3 cut(s) 74, 782, 793
CviQI GTAC 3 cut(s) 234, 474, 510
DdeI CTNAG 2 cut(s) 190, 807
DpnI GATC 5 cut(s) 60, 317, 325, 569, 737
DpnII GATC 5 cut(s) 58, 315, 323, 567, 735
Eam1104I CTCTTC 3 cut(s) 488, 530, 692
EarI CTCTTC 3 cut(s) 488, 530, 692
Ecl136II GAGCTC 1 cut(s) 270
Eco24I GRGCYC 3 cut(s) 272, 452, 555
Eco47III AGCGCT 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 3 cut(s) 187, 279, 545
Eco81I CCTNAGG 1 cut(s) 807
Eco88I CYCGRG 1 cut(s) 554
Eco91I GGTNACC 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 270
EcoO65I GGTNACC 1 cut(s) 594
EcoT38I GRGCYC 3 cut(s) 272, 452, 555
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 3 cut(s) 77, 785, 796
FaiI YATR 7 cut(s) 14, 75, 372, 374, 577, 783, 794
FalI AAGNNNNNCTT 2 cut(s) 274, 306
FaqI GGGAC 1 cut(s) 12
FatI CATG 3 cut(s) 73, 781, 792
FbaI TGATCA 1 cut(s) 58
Fnu4HI GCNGC 6 cut(s) 286, 417, 420, 440, 443, 488
FriOI GRGCYC 3 cut(s) 272, 452, 555
Fsp4HI GCNGC 6 cut(s) 286, 417, 420, 440, 443, 488
FspBI CTAG 3 cut(s) 228, 582, 651
GlaI GCGC 1 cut(s) 289
GluI GCNGC 6 cut(s) 286, 417, 420, 440, 443, 488
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 1 cut(s) 688
HapII CCGG 2 cut(s) 502, 647
HgaI GACGC 1 cut(s) 493
HhaI GCGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 3 cut(s) 77, 785, 796
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 586
HinfI GANTC 4 cut(s) 498, 700, 727, 797
HpaII CCGG 2 cut(s) 502, 647
HphI GGTGA 4 cut(s) 128, 147, 507, 588
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 4 cut(s) 525, 606, 628, 657
Hpy188III TCNNGA 5 cut(s) 97, 502, 582, 782, 806
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 1 cut(s) 487
HpyAV CCTTC 5 cut(s) 141, 174, 183, 211, 606
HpyCH4III ACNGT 1 cut(s) 600
HpyCH4IV ACGT 1 cut(s) 425
HpyCH4V TGCA 5 cut(s) 12, 24, 69, 416, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 559
HpyF3I CTNAG 2 cut(s) 190, 807
HpySE526I ACGT 1 cut(s) 425
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 3 cut(s) 77, 785, 796
HspAI GCGC 1 cut(s) 288
Kpn2I TCCGGA 1 cut(s) 501
KroI GCCGGC 1 cut(s) 646
KroNI GCCGGC 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 58
Kzo9I GATC 5 cut(s) 58, 315, 323, 567, 735
Lsp1109I GCAGC 4 cut(s) 297, 428, 451, 454
MaeI CTAG 3 cut(s) 228, 582, 651
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 1 cut(s) 594
MalI GATC 5 cut(s) 60, 317, 325, 569, 737
MboI GATC 5 cut(s) 58, 315, 323, 567, 735
MboII GAAGA 9 cut(s) 62, 65, 159, 189, 285, 505, 547, 643, 709
MhlI GDGCHC 3 cut(s) 272, 452, 555
MluCI AATT 4 cut(s) 62, 126, 178, 241
MlyI GAGTC 3 cut(s) 507, 709, 806
MnlI CCTC 6 cut(s) 117, 199, 455, 585, 802, 834
MroI TCCGGA 1 cut(s) 501
MroNI GCCGGC 1 cut(s) 646
MroXI GAANNNNTTC 2 cut(s) 338, 539
MseI TTAA 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 2 cut(s) 502, 647
MwoI GCNNNNNNNGC 2 cut(s) 267, 559
NaeI GCCGGC 1 cut(s) 648
NdeII GATC 5 cut(s) 58, 315, 323, 567, 735
NgoMIV GCCGGC 1 cut(s) 646
NlaIII CATG 3 cut(s) 77, 785, 796
NmeAIII GCCGAG 2 cut(s) 471, 664
NmuCI GTSAC 1 cut(s) 594
NspV TTCGAA 1 cut(s) 332
PagI TCATGA 1 cut(s) 781
PdiI GCCGGC 1 cut(s) 648
PdmI GAANNNNTTC 2 cut(s) 338, 539
PfeI GAWTC 1 cut(s) 727
PflMI CCANNNNNTGG 1 cut(s) 793
PkrI GCNGC 6 cut(s) 287, 418, 421, 441, 444, 489
PleI GAGTC 3 cut(s) 506, 708, 805
PpsI GAGTC 3 cut(s) 506, 708, 805
Psp124BI GAGCTC 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 425
PspEI GGTNACC 1 cut(s) 594
RsaI GTAC 3 cut(s) 235, 475, 511
RsaNI GTAC 3 cut(s) 234, 474, 510
SacI GAGCTC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 677
SatI GCNGC 6 cut(s) 286, 417, 420, 440, 443, 488
Sau3AI GATC 5 cut(s) 58, 315, 323, 567, 735
ScaI AGTACT 2 cut(s) 235, 511
SchI GAGTC 3 cut(s) 507, 709, 806
SduI GDGCHC 3 cut(s) 272, 452, 555
SfuI TTCGAA 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 589
SmoI CTYRAG 1 cut(s) 589
SpeI ACTAGT 1 cut(s) 227
Sse9I AATT 4 cut(s) 62, 126, 178, 241
SsiI CCGC 2 cut(s) 419, 488
SspMI CTAG 3 cut(s) 228, 582, 651
SstI GAGCTC 1 cut(s) 272
TaaI ACNGT 1 cut(s) 600
TaiI ACGT 1 cut(s) 428
TaqI TCGA 7 cut(s) 48, 98, 266, 302, 318, 332, 750
TasI AATT 4 cut(s) 62, 126, 178, 241
TatI WGTACW 3 cut(s) 233, 473, 509
TauI GCSGC 2 cut(s) 422, 490
TfiI GAWTC 1 cut(s) 727
Tru1I TTAA 1 cut(s) 677
Tru9I TTAA 1 cut(s) 677
TseFI GTSAC 1 cut(s) 594
TseI GCWGC 4 cut(s) 285, 416, 439, 442
Tsp45I GTSAC 1 cut(s) 594
TspDTI ATGAA 1 cut(s) 768
TspGWI ACGGA 1 cut(s) 533
Van91I CCANNNNNTGG 1 cut(s) 793
XapI RAATTY 1 cut(s) 178
XbaI TCTAGA 1 cut(s) 581
XmnI GAANNNNTTC 2 cut(s) 338, 539
XspI CTAG 3 cut(s) 228, 582, 651
ZrmI AGTACT 2 cut(s) 235, 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.