AT1G01630

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011976
Physical Location & Seq
Forward (+)
228799 .. 230979
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01630.1

Sequence Viewer

Length: 768 bp
ATGGAAAACAAAGAGACCAAGCAAGAACCAGCAGCAGCAGCTGAGCAAAAGACCGTTCCGTTGATCGAAGATGAGATCGAGAGGAGCAAAGTCGGGATCATGAGAGCTCTCTGCGACCGACAAGATCCTGAAACTAAGGAGGTGGATGATCTGATGATAAGGAGGTTTCTGAGAGCGCGTGACCTGGACATTGAAAAGGCTTCAACGATGTTTCTAAATTACCTGACTTGGAAGAGAAGCATGCTCCCAAAGGGGCACATACCAGAAGCAGAGATTGCAAATGATCTATCGCATAACAAGATGTGTATGCAAGGTCATGACAAGATGGGTCGACCTATCGCTGTTGCCATTGGGAACAGACATAACCCTTCCAAAGGCAACCCTGACGAGTTCAAGCGTTTTGTTGTCTACACGCTCGAGAAGATTTGTGCTAGAATGCCGAGAGGTCAAGAGAAATTCGTAGCAATTGGAGATCTGCAAGGCTGGGGATATTCTAATTGTGACATCCGTGGCTACCTTGCTGCTCTTTCCACTTTGCAGGATTGTTACCCAGAGAGATTAGGGAAACTCTATATAGTTCATGCCCCCTACATTTTCATGACCGCATGGAAGGTCATTTATCCTTTTATCGACGCCAACACCAAGAAAAAGATTGTTTTCGTGGAGAACAAGAAACTCACTCCAACGCTGCTTGAAGACATAGACGAAAGCCAACTTCCCGACATCTACGGAGGCAAATTGCCACTTGTTCCTATTCAGGAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

255

Amino Acids

29.22

Weight (kDa)

6.96

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 97 - 245 2.3e-39 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 110 - 245 1.1e-09 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 331, 408
AccII CGCG 1 cut(s) 178
AciI CCGC 1 cut(s) 603
AclWI GGATC 2 cut(s) 104, 119
AcsI RAATTY 1 cut(s) 455
AcyI GRCGYC 1 cut(s) 633
AfiI CCNNNNNNNGG 1 cut(s) 374
AgsI TTSAA 4 cut(s) 194, 204, 394, 695
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 2 cut(s) 41, 107
AluI AGCT 2 cut(s) 41, 107
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 2 cut(s) 8, 755
AlwI GGATC 2 cut(s) 104, 119
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 416
ApeKI GCWGC 5 cut(s) 32, 35, 38, 521, 688
ApoI RAATTY 1 cut(s) 455
AspLEI GCGC 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 258
BanII GRGCYC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 5 cut(s) 44, 47, 50, 508, 675
BccI CCATC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 8, 755
BfaI CTAG 1 cut(s) 432
BglII AGATCT 1 cut(s) 472
BisI GCNGC 5 cut(s) 33, 36, 39, 522, 689
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 5 cut(s) 34, 37, 40, 523, 690
Bme1390I CCNGG 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 416
BmrFI CCNGG 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 702
Bpu1102I GCTNAGC 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 633
BsaI GGTCTC 2 cut(s) 8, 755
BsaJI CCNNGG 1 cut(s) 508
Bsc4I CCNNNNNNNGG 1 cut(s) 374
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 2 cut(s) 151, 504
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMII CTCAG 2 cut(s) 33, 161
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 5 cut(s) 44, 47, 50, 508, 675
BseYI CCCAGC 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 178
Bsh1285I CGRYCG 1 cut(s) 118
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 109
BsiHKCI CYCGRG 1 cut(s) 416
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 2 cut(s) 8, 755
BsmI GAATGC 1 cut(s) 441
Bso31I GGTCTC 2 cut(s) 8, 755
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 2 cut(s) 109, 258
Bsp143I GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 1 cut(s) 603
BspCNI CTCAG 2 cut(s) 34, 162
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 3 cut(s) 99, 316, 597
BspPI GGATC 2 cut(s) 104, 119
BspTNI GGTCTC 2 cut(s) 8, 755
BssECI CCNNGG 1 cut(s) 508
BssMI GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
BssNI GRCGYC 1 cut(s) 633
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 633
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 3 cut(s) 42, 135, 170
BstDSI CCRYGG 1 cut(s) 508
BstENI CCTNNNNNAGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 151, 504
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 7 cut(s) 66, 78, 99, 127, 151, 286, 475
BstMAI GTCTC 2 cut(s) 8, 755
BstMBI GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 2 cut(s) 38, 275
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 5 cut(s) 44, 47, 50, 508, 675
BstV2I GAAGAC 1 cut(s) 702
BstX2I RGATCY 2 cut(s) 124, 472
BstYI RGATCY 2 cut(s) 124, 472
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 2 cut(s) 151, 504
Cac8I GCNNGC 1 cut(s) 242
CaiI CAGNNNCTG 1 cut(s) 41
CciI TCATGA 3 cut(s) 99, 316, 597
CfoI GCGC 1 cut(s) 178
CseI GACGC 1 cut(s) 641
CviAII CATG 6 cut(s) 100, 241, 317, 581, 598, 606
CviJI RGCY 6 cut(s) 41, 107, 200, 483, 513, 711
CviKI_1 RGCY 6 cut(s) 41, 107, 200, 483, 513, 711
DdeI CTNAG 3 cut(s) 42, 135, 170
DpnI GATC 7 cut(s) 65, 77, 98, 126, 150, 285, 474
DpnII GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Ecl136II GAGCTC 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 109
Eco31I GGTCTC 2 cut(s) 8, 755
Eco53kI GAGCTC 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 109
FaeI CATG 6 cut(s) 103, 244, 320, 584, 601, 609
FatI CATG 6 cut(s) 99, 240, 316, 580, 597, 605
FblI GTMKAC 2 cut(s) 331, 408
Fnu4HI GCNGC 5 cut(s) 33, 36, 39, 522, 689
FokI GGATG 2 cut(s) 158, 491
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 5 cut(s) 33, 36, 39, 522, 689
FspBI CTAG 1 cut(s) 432
GlaI GCGC 1 cut(s) 177
GluI GCNGC 5 cut(s) 33, 36, 39, 522, 689
GsaI CCCAGC 1 cut(s) 487
HgaI GACGC 1 cut(s) 641
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 6 cut(s) 103, 244, 320, 584, 601, 609
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 1 cut(s) 332
HindII GTYRAC 1 cut(s) 332
Hpy166II GTNNAC 2 cut(s) 332, 409
Hpy188I TCNGA 2 cut(s) 153, 171
Hpy8I GTNNAC 2 cut(s) 332, 409
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 2 cut(s) 378, 604
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4V TGCA 4 cut(s) 278, 310, 478, 538
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 275
HpyF3I CTNAG 3 cut(s) 42, 135, 170
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 6 cut(s) 103, 244, 320, 584, 601, 609
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
LmnI GCTCC 2 cut(s) 84, 249
Lsp1109I GCAGC 5 cut(s) 44, 47, 50, 508, 675
MaeI CTAG 1 cut(s) 432
MaeIII GTNAC 3 cut(s) 179, 500, 545
MalI GATC 7 cut(s) 65, 77, 98, 126, 150, 285, 474
MboI GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
MboII GAAGA 4 cut(s) 80, 244, 433, 707
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 2 cut(s) 124, 472
MhlI GDGCHC 2 cut(s) 109, 258
MluCI AATT 5 cut(s) 217, 455, 465, 496, 737
MmeI TCCRAC 1 cut(s) 707
MnlI CCTC 5 cut(s) 75, 133, 156, 437, 725
MslI CAYNNNNRTG 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 185
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 2 cut(s) 38, 275
NdeII GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
NlaIII CATG 6 cut(s) 103, 244, 320, 584, 601, 609
NmeAIII GCCGAG 1 cut(s) 465
NmuCI GTSAC 2 cut(s) 179, 500
NspI RCATGY 1 cut(s) 244
PaeI GCATGC 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 416
PagI TCATGA 3 cut(s) 99, 316, 597
PctI GAATGC 1 cut(s) 441
PkrI GCNGC 5 cut(s) 34, 37, 40, 523, 690
Psp124BI GAGCTC 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 483
PspGI CCWGG 1 cut(s) 183
PstNI CAGNNNCTG 1 cut(s) 41
PsuI RGATCY 2 cut(s) 124, 472
PvuII CAGCTG 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 596
SacI GAGCTC 1 cut(s) 109
SalI GTCGAC 1 cut(s) 330
SatI GCNGC 5 cut(s) 33, 36, 39, 522, 689
Sau3AI GATC 7 cut(s) 63, 75, 96, 124, 148, 283, 472
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 2 cut(s) 109, 258
Sfr274I CTCGAG 1 cut(s) 416
SlaI CTCGAG 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SphI GCATGC 1 cut(s) 244
Sse9I AATT 5 cut(s) 217, 455, 465, 496, 737
SsiI CCGC 1 cut(s) 603
SspMI CTAG 1 cut(s) 432
SstI GAGCTC 1 cut(s) 109
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 1 cut(s) 55
TaqI TCGA 5 cut(s) 66, 78, 331, 417, 630
TaqII GACCGA 1 cut(s) 132
TasI AATT 5 cut(s) 217, 455, 465, 496, 737
TseFI GTSAC 2 cut(s) 179, 500
TseI GCWGC 5 cut(s) 32, 35, 38, 521, 688
Tsp45I GTSAC 2 cut(s) 179, 500
TspDTI ATGAA 2 cut(s) 569, 586
TspGWI ACGGA 3 cut(s) 48, 497, 744
XagI CCTNNNNNAGG 1 cut(s) 372
XapI RAATTY 1 cut(s) 455
XceI RCATGY 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 416
XmiI GTMKAC 2 cut(s) 331, 408
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.