Prupe.1G214400_v2.0.a1

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011976
Physical Location & Seq
Forward (+)
22758366 .. 22760863
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G214400.1

Sequence Viewer

Length: 789 bp
ATGGAGTCATCCAATGGGAAAGAGAGTGTGATGAGCATGGAAATAAATGAGGGGATGGAAGTAAATGAGGGGATCGAAGAATCAAACTTTGAAAGCAATGAGATTGAGAGGAACAAGATGGGCATCATGAGAGCTCTTGTGGAAAGGGAAGATCCCTCCTCCAAGGACGTGGATGATTTCATGATCCGGAGATTTCTGCGGGCACGTGATCTGGATATTGAGAAGGCTTCTACCCTGTTCCTCAAGTATCTGAGTTGGAGAAAGTCATTTGTGCCAAATGGTTCCATCTCAGAGTCAGAGATTCCAAGAGAGCTTGCTCATAACAAGCTGTTTATGCAAGGTCTGGACAAGACGGGACGCCCCATCGTCGTCGTTTTTGGTGGGAGGCATAAGCGCACCAAACTAGAGGAGTTCAAACGTTTTGTAGTCTATAGCCTTGACAAAATAAGTGCCAGAATGCCAGCAGGCAAGGAAAAGTTTGTATCCATTGCAGATCTTGAAGGATGGGGATATGTCAATAGCGACATTCGTGGATACTTGGCAGCTCTGTCGATCTTGCAGGATTGCTACCCAGAAAGGCTTGGCAAGTTATATCTTGTTCATGTGCCTTACGTTTTCATGACGGCATGGAAGATGGTTTACCCATTTATTGACAACAAAACCAAGAAGAAGATAGTTTTTGTTGAGAACAAAAAACTGAGCTCCACCCTGCTCAGTGATATTGATGAGAGCCAACTCCCAGATACATATGGAGGCAAATTGCCGTTAGTTCCTATCCAAGACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

263

Amino Acids

30.1

Weight (kDa)

6.38

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 1 cut(s) 199
AclI AACGTT 1 cut(s) 418
AclWI GGATC 3 cut(s) 80, 146, 178
AcvI CACGTG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 358
AgsI TTSAA 3 cut(s) 92, 415, 500
AjiI CACGTC 1 cut(s) 169
AluBI AGCT 5 cut(s) 134, 313, 328, 545, 702
AluI AGCT 5 cut(s) 134, 313, 328, 545, 702
Alw21I GWGCWC 2 cut(s) 136, 704
AlwI GGATC 3 cut(s) 80, 146, 178
Aor13HI TCCGGA 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 542
AspLEI GCGC 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 205
BanII GRGCYC 2 cut(s) 136, 704
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BbrPI CACGTG 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 136, 704
BbvI GCAGC 1 cut(s) 554
BccI CCATC 6 cut(s) 49, 112, 293, 371, 498, 628
BceAI ACGGC 2 cut(s) 639, 748
BcgI CGANNNNNNTGC 2 cut(s) 531, 565
BciVI GTATCC 2 cut(s) 493, 527
BfaI CTAG 2 cut(s) 404, 787
BfmI CTRYAG 1 cut(s) 430
BfuI GTATCC 2 cut(s) 493, 527
BglII AGATCT 1 cut(s) 493
BisI GCNGC 1 cut(s) 543
BlsI GCNGC 1 cut(s) 544
BmgBI CACGTC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 283
BmsI GCATC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 186
Bse3DI GCAATG 2 cut(s) 103, 486
Bse8I GATNNNNATC 1 cut(s) 122
BseAI TCCGGA 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 4 cut(s) 8, 60, 178, 509
BseJI GATNNNNATC 1 cut(s) 122
BseMI GCAATG 2 cut(s) 103, 486
BseMII CTCAG 4 cut(s) 242, 303, 689, 727
BseRI GAGGAG 2 cut(s) 148, 422
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 1 cut(s) 554
BsiHKAI GWGCWC 2 cut(s) 136, 704
BsiSI CCGG 1 cut(s) 187
BslFI GGGAC 1 cut(s) 369
BsmFI GGGAC 1 cut(s) 369
BsmI GAATGC 1 cut(s) 462
Bsp1286I GDGCHC 3 cut(s) 136, 205, 704
Bsp13I TCCGGA 1 cut(s) 186
Bsp143I GATC 6 cut(s) 72, 151, 183, 208, 493, 552
BspACI CCGC 1 cut(s) 199
BspCNI CTCAG 4 cut(s) 243, 302, 690, 726
BspEI TCCGGA 1 cut(s) 186
BspHI TCATGA 3 cut(s) 126, 180, 618
BspLI GGNNCC 1 cut(s) 283
BspPI GGATC 3 cut(s) 80, 146, 178
BsrDI GCAATG 2 cut(s) 103, 486
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 6 cut(s) 72, 151, 183, 208, 493, 552
BssNI GRCGYC 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 358
BstBAI YACGTR 1 cut(s) 206
BstC8I GCNNGC 4 cut(s) 201, 315, 462, 466
BstDEI CTNAG 4 cut(s) 251, 289, 698, 713
BstF5I GGATG 4 cut(s) 8, 60, 178, 509
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 6 cut(s) 75, 154, 186, 211, 496, 555
BstMBI GATC 6 cut(s) 72, 151, 183, 208, 493, 552
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstSFI CTRYAG 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 554
BstX2I RGATCY 2 cut(s) 151, 493
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 2 cut(s) 151, 493
BsuI GTATCC 2 cut(s) 493, 527
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 4 cut(s) 8, 60, 178, 509
BtsIMutI CAGTG 1 cut(s) 721
Cac8I GCNNGC 4 cut(s) 201, 315, 462, 466
CciI TCATGA 3 cut(s) 126, 180, 618
CfoI GCGC 1 cut(s) 396
CseI GACGC 1 cut(s) 366
CviAII CATG 6 cut(s) 37, 127, 181, 602, 619, 627
CviJI RGCY 9 cut(s) 134, 227, 313, 328, 435, 545, 580, 702, 732
CviKI_1 RGCY 9 cut(s) 134, 227, 313, 328, 435, 545, 580, 702, 732
DdeI CTNAG 4 cut(s) 251, 289, 698, 713
DpnI GATC 6 cut(s) 74, 153, 185, 210, 495, 554
DpnII GATC 6 cut(s) 72, 151, 183, 208, 493, 552
Ecl136II GAGCTC 2 cut(s) 134, 702
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 2 cut(s) 136, 704
Eco53kI GAGCTC 2 cut(s) 134, 702
Eco72I CACGTG 1 cut(s) 206
EcoICRI GAGCTC 2 cut(s) 134, 702
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 2 cut(s) 136, 704
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 6 cut(s) 40, 130, 184, 605, 622, 630
FaqI GGGAC 1 cut(s) 369
FatI CATG 6 cut(s) 36, 126, 180, 601, 618, 626
FauI CCCGC 1 cut(s) 192
FauNDI CATATG 1 cut(s) 748
Fnu4HI GCNGC 1 cut(s) 543
FokI GGATG 3 cut(s) 67, 185, 516
FriOI GRGCYC 2 cut(s) 136, 704
Fsp4HI GCNGC 1 cut(s) 543
FspBI CTAG 2 cut(s) 404, 787
GlaI GCGC 1 cut(s) 395
GluI GCNGC 1 cut(s) 543
HapII CCGG 1 cut(s) 187
HgaI GACGC 1 cut(s) 366
HhaI GCGC 1 cut(s) 396
Hin1I GRCGYC 1 cut(s) 358
Hin1II CATG 6 cut(s) 40, 130, 184, 605, 622, 630
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HinfI GANTC 4 cut(s) 5, 80, 293, 301
HpaII CCGG 1 cut(s) 187
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 3 cut(s) 252, 292, 298
Hpy188III TCNNGA 7 cut(s) 127, 181, 187, 212, 344, 497, 619
Hpy8I GTNNAC 1 cut(s) 640
Hpy99I CGWCG 2 cut(s) 371, 374
HpyAV CCTTC 2 cut(s) 217, 494
HpyCH4IV ACGT 4 cut(s) 168, 205, 418, 612
HpyCH4V TGCA 3 cut(s) 337, 491, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 4 cut(s) 251, 289, 698, 713
HpySE526I ACGT 4 cut(s) 168, 205, 418, 612
Hsp92I GRCGYC 1 cut(s) 358
Hsp92II CATG 6 cut(s) 40, 130, 184, 605, 622, 630
HspAI GCGC 1 cut(s) 394
Kpn2I TCCGGA 1 cut(s) 186
Kzo9I GATC 6 cut(s) 72, 151, 183, 208, 493, 552
LmnI GCTCC 1 cut(s) 707
Lsp1109I GCAGC 1 cut(s) 554
LweI GCATC 1 cut(s) 132
MaeI CTAG 2 cut(s) 404, 787
MaeII ACGT 4 cut(s) 168, 205, 418, 612
MalI GATC 6 cut(s) 74, 153, 185, 210, 495, 554
MboI GATC 6 cut(s) 72, 151, 183, 208, 493, 552
MboII GAAGA 5 cut(s) 89, 161, 643, 679, 682
MflI RGATCY 2 cut(s) 151, 493
MhlI GDGCHC 3 cut(s) 136, 205, 704
MluCI AATT 1 cut(s) 758
MlyI GAGTC 2 cut(s) 14, 302
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 9 cut(s) 43, 61, 102, 166, 169, 251, 378, 400, 746
MroI TCCGGA 1 cut(s) 186
MspI CCGG 1 cut(s) 187
Mva1269I GAATGC 1 cut(s) 462
MwoI GCNNNNNNNGC 1 cut(s) 334
NdeI CATATG 1 cut(s) 748
NdeII GATC 6 cut(s) 72, 151, 183, 208, 493, 552
NlaIII CATG 6 cut(s) 40, 130, 184, 605, 622, 630
NlaIV GGNNCC 1 cut(s) 283
PagI TCATGA 3 cut(s) 126, 180, 618
PctI GAATGC 1 cut(s) 462
PfeI GAWTC 2 cut(s) 80, 301
PkrI GCNGC 1 cut(s) 544
PleI GAGTC 2 cut(s) 13, 301
PmaCI CACGTG 1 cut(s) 206
PmlI CACGTG 1 cut(s) 206
PpsI GAGTC 2 cut(s) 13, 301
Ppu21I YACGTR 1 cut(s) 206
Psp124BI GAGCTC 2 cut(s) 136, 704
Psp1406I AACGTT 1 cut(s) 418
PspCI CACGTG 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 283
PsuI RGATCY 2 cut(s) 151, 493
SacI GAGCTC 2 cut(s) 136, 704
SatI GCNGC 1 cut(s) 543
Sau3AI GATC 6 cut(s) 72, 151, 183, 208, 493, 552
SchI GAGTC 2 cut(s) 14, 302
SduI GDGCHC 3 cut(s) 136, 205, 704
SfaNI GCATC 1 cut(s) 132
SfcI CTRYAG 1 cut(s) 430
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 1 cut(s) 758
SsiI CCGC 1 cut(s) 199
SspMI CTAG 2 cut(s) 404, 787
SstI GAGCTC 2 cut(s) 136, 704
StyI CCWWGG 1 cut(s) 162
TaiI ACGT 4 cut(s) 171, 208, 421, 615
TaqI TCGA 2 cut(s) 75, 551
TasI AATT 1 cut(s) 758
TfiI GAWTC 2 cut(s) 80, 301
TscAI CASTG 1 cut(s) 721
TseI GCWGC 1 cut(s) 542
TspDTI ATGAA 3 cut(s) 169, 590, 607
TspRI CASTG 1 cut(s) 721
XspI CTAG 2 cut(s) 404, 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.