Rh4CG308000

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011976
Physical Location & Seq
Forward (+)
56975087 .. 56980432
5346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG308000.1

Sequence Viewer

Length: 639 bp
ATGGAATCCAATGGGAAAGATGAGGGAATGGAAGAGCAACAGAGCTTGGAAAGCAGTGAGATTGAGAAAAGCAAAATGGGGATCATGAGAGCCCTTGTGGAAAGAGAAGATCCCTCCTCCAAGGAAGTGGATGATTTCATGATCCGGAGATTTCTTCGGGCTCGTGATCATGACATTGAGAAGGCTTCTAACCTCTTCCTCAAGTACCTGAGTTGGAGGAAAGCATTTATGCCAAATGGTTCCATCTCAGAGTCGGAGATTCCAAGAGAACTAGCTCATAACAAGTTGTTTATGCAAGGCGTGGACAAGACGGGGCGCCCCATTGTTGTTGTCTATGGTGGGAGGCATAAGCACACCAAGCTAGAGGAGTTTAAACGTTTTGTAGTCTACACTCTTGAGAAGATATGTGCCAGAATGCCAGCGGGGCAGGAAAAGTTTTTATCCATTGCAGATATTGAAGGATGGGGGTATGCCAACAGTGATGTTCGTGGATACTTAGCAGCTCTAACAATCTTACAGGATTGCTATCCGGAGAGGCTTGGCAAGTTATTTATTGTCCATTCACCGTATCTATTCATGACTGCTTGGAAGATGGTTTACCCTTTTATCGATGACAAAACCAAAAAAAAGGTAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

212

Amino Acids

24.63

Weight (kDa)

8.69

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 93 - 212 3.4e-29 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 315
AccI GTMKAC 1 cut(s) 387
AccIII TCCGGA 2 cut(s) 144, 529
AciI CCGC 1 cut(s) 422
AclI AACGTT 1 cut(s) 376
AclWI GGATC 3 cut(s) 89, 104, 136
AcyI GRCGYC 1 cut(s) 316
AfaI GTAC 1 cut(s) 206
AgsI TTSAA 1 cut(s) 458
AluBI AGCT 4 cut(s) 45, 275, 361, 503
AluI AGCT 4 cut(s) 45, 275, 361, 503
AlwI GGATC 3 cut(s) 89, 104, 136
Aor13HI TCCGGA 2 cut(s) 144, 529
ApeKI GCWGC 1 cut(s) 500
AspLEI GCGC 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 555
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 2 cut(s) 94, 163
BarI GAAGNNNNNNTAC 2 cut(s) 581, 613
BauI CACGAG 1 cut(s) 162
BbvI GCAGC 1 cut(s) 512
BccI CCATC 3 cut(s) 251, 456, 586
BciVI GTATCC 1 cut(s) 485
BclI TGATCA 1 cut(s) 166
BfaI CTAG 2 cut(s) 272, 362
BfoI RGCGCY 1 cut(s) 319
BfuI GTATCC 1 cut(s) 485
BglI GCCNNNNNGGC 1 cut(s) 424
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
BmiI GGNNCC 2 cut(s) 241, 317
BpuEI CTTGAG 2 cut(s) 185, 416
Bsa29I ATCGAT 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 120
BsaWI WCCGGW 2 cut(s) 144, 529
Bse3DI GCAATG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 525
BseAI TCCGGA 2 cut(s) 144, 529
BseCI ATCGAT 1 cut(s) 609
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 136, 467
BseJI GATNNNNATC 1 cut(s) 525
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 2 cut(s) 200, 261
BseRI GAGGAG 2 cut(s) 106, 380
BseXI GCAGC 1 cut(s) 512
BshNI GGYRCC 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 609
BsiSI CCGG 2 cut(s) 145, 530
BsmI GAATGC 1 cut(s) 420
Bsp1286I GDGCHC 2 cut(s) 94, 163
Bsp13I TCCGGA 2 cut(s) 144, 529
Bsp143I GATC 4 cut(s) 81, 109, 141, 166
BspACI CCGC 1 cut(s) 422
BspCNI CTCAG 2 cut(s) 201, 260
BspDI ATCGAT 1 cut(s) 609
BspEI TCCGGA 2 cut(s) 144, 529
BspHI TCATGA 4 cut(s) 84, 138, 169, 576
BspLI GGNNCC 2 cut(s) 241, 317
BspPI GGATC 3 cut(s) 89, 104, 136
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 315
BsrDI GCAATG 1 cut(s) 444
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 4 cut(s) 81, 109, 141, 166
BssNI GRCGYC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 120
Bst2BI CACGAG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 479, 567
Bst6I CTCTTC 2 cut(s) 27, 200
BstACI GRCGYC 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 3 cut(s) 209, 247, 496
BstF5I GGATG 2 cut(s) 136, 467
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 4 cut(s) 84, 112, 144, 169
BstMBI GATC 4 cut(s) 81, 109, 141, 166
BstMWI GCNNNNNNNGC 3 cut(s) 51, 358, 424
BstV1I GCAGC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 109
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 609
BsuI GTATCC 1 cut(s) 485
BsuTUI ATCGAT 1 cut(s) 609
BtsCI GGATG 2 cut(s) 136, 467
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 484
Cac8I GCNNGC 1 cut(s) 420
CciI TCATGA 4 cut(s) 84, 138, 169, 576
CfoI GCGC 1 cut(s) 318
ClaI ATCGAT 1 cut(s) 609
Csp6I GTAC 1 cut(s) 205
CviAII CATG 4 cut(s) 85, 139, 170, 577
CviJI RGCY 8 cut(s) 45, 92, 161, 185, 275, 361, 503, 538
CviKI_1 RGCY 8 cut(s) 45, 92, 161, 185, 275, 361, 503, 538
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 3 cut(s) 209, 247, 496
DinI GGCGCC 1 cut(s) 317
DpnI GATC 4 cut(s) 83, 111, 143, 168
DpnII GATC 4 cut(s) 81, 109, 141, 166
DraI TTTAAA 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 27, 200
EarI CTCTTC 2 cut(s) 27, 200
Eco130I CCWWGG 1 cut(s) 120
Eco24I GRGCYC 2 cut(s) 94, 163
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 2 cut(s) 94, 163
EgeI GGCGCC 1 cut(s) 317
EheI GGCGCC 1 cut(s) 317
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 4 cut(s) 88, 142, 173, 580
FatI CATG 4 cut(s) 84, 138, 169, 576
FauI CCCGC 1 cut(s) 415
FbaI TGATCA 1 cut(s) 166
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 2 cut(s) 143, 474
FriOI GRGCYC 2 cut(s) 94, 163
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 2 cut(s) 272, 362
GlaI GCGC 1 cut(s) 317
GluI GCNGC 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 319
HapII CCGG 2 cut(s) 145, 530
HhaI GCGC 1 cut(s) 318
Hin1I GRCGYC 1 cut(s) 316
Hin1II CATG 4 cut(s) 88, 142, 173, 580
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 3 cut(s) 5, 251, 259
HpaII CCGG 2 cut(s) 145, 530
HphI GGTGA 1 cut(s) 555
Hpy166II GTNNAC 3 cut(s) 304, 388, 598
Hpy188I TCNGA 2 cut(s) 250, 256
Hpy188III TCNNGA 8 cut(s) 85, 139, 145, 164, 170, 395, 530, 577
Hpy8I GTNNAC 3 cut(s) 304, 388, 598
HpyAV CCTTC 2 cut(s) 175, 452
HpyCH4III ACNGT 2 cut(s) 479, 567
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 2 cut(s) 295, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 358, 424
HpyF3I CTNAG 3 cut(s) 209, 247, 496
HpySE526I ACGT 1 cut(s) 376
Hsp92I GRCGYC 1 cut(s) 316
Hsp92II CATG 4 cut(s) 88, 142, 173, 580
HspAI GCGC 1 cut(s) 316
KasI GGCGCC 1 cut(s) 315
Kpn2I TCCGGA 2 cut(s) 144, 529
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 4 cut(s) 81, 109, 141, 166
LguI GCTCTTC 1 cut(s) 27
LpnPI CCDG 7 cut(s) 158, 221, 413, 424, 432, 503, 543
Lsp1109I GCAGC 1 cut(s) 512
MaeI CTAG 2 cut(s) 272, 362
MaeII ACGT 1 cut(s) 376
MalI GATC 4 cut(s) 83, 111, 143, 168
MboI GATC 4 cut(s) 81, 109, 141, 166
MboII GAAGA 6 cut(s) 44, 119, 146, 187, 412, 601
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 94, 163
Mly113I GGCGCC 1 cut(s) 316
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 194, 234
MnlI CCTC 9 cut(s) 16, 124, 127, 203, 209, 210, 336, 358, 528
MroI TCCGGA 2 cut(s) 144, 529
MseI TTAA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 2 cut(s) 145, 530
MssI GTTTAAAC 1 cut(s) 373
Mva1269I GAATGC 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 51, 358, 424
NarI GGCGCC 1 cut(s) 316
NdeII GATC 4 cut(s) 81, 109, 141, 166
NlaIII CATG 4 cut(s) 88, 142, 173, 580
NlaIV GGNNCC 2 cut(s) 241, 317
PagI TCATGA 4 cut(s) 84, 138, 169, 576
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 5, 259
PkrI GCNGC 1 cut(s) 502
PleI GAGTC 1 cut(s) 259
PluTI GGCGCC 1 cut(s) 319
PmeI GTTTAAAC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 259
Psp1406I AACGTT 1 cut(s) 376
PspN4I GGNNCC 2 cut(s) 241, 317
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 4 cut(s) 81, 109, 141, 166
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 2 cut(s) 94, 163
SetI ASST 8 cut(s) 47, 195, 210, 277, 363, 379, 505, 633
SfoI GGCGCC 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 200, 395
SmoI CTYRAG 2 cut(s) 200, 395
SsiI CCGC 1 cut(s) 422
SspDI GGCGCC 1 cut(s) 315
SspMI CTAG 2 cut(s) 272, 362
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 479, 567
TaiI ACGT 1 cut(s) 379
TaqI TCGA 1 cut(s) 609
TfiI GAWTC 2 cut(s) 5, 259
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 2 cut(s) 61, 484
TseI GCWGC 1 cut(s) 500
TspDTI ATGAA 2 cut(s) 127, 565
TspRI CASTG 2 cut(s) 61, 484
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 2 cut(s) 272, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.