Rroxscaffold_5G00370510

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011976
Physical Location & Seq
Forward (+)
52212357 .. 52215199
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370510.1

Sequence Viewer

Length: 645 bp
ATGGAATCCAATGGGAAAGATGAGGGACTGGAAGAGCAACAGAGCTTGGAAAGCAGTGAGATTGAGAAAAGCCAAGTGGGGATCATGAGAGCCCTTGTGGAAAGAGAAGATCCCTCCTCCAAGGAAGTGGATGATTTCATGATCCGGAGATTTCTTCGGGCTCGTGATCATGACATCGAGAAGGCTTCTAACCTCTTCCTCAAGTACCTGAGTTGGAGGAAAGCATTTATGCCAAATGGTTCCATCTCAGAGTCGGAGATTCCAAGAGAACTAGCTCATAACAAGTTGTTTATGCAAGGCGTGGACAAGAAGGGGCGCCCCATTGTTGTTGTCTATGGTGGGAGGCATAAGCACACCAAGCTAGAGGAGTTTAAACGTTTTGTAGTCTACACTCTTGAGAAGATATGTGCCAGAATGCCAGTGGGGCAGGAAAAGTTTTTATCCATTGCAGATCTTGAAGGATGGGGGTATGCCAATAGTGATGTTCGTGGATACTTAGCAGCTCTAACAATCTTACAGGATTGCTATCCGGAGAGGCTTGGCAAGTTATTTATTGTCCATTCACCGTACCTATTCATGACTGCTTGGAAGATGGTTTACCCTTTTATCGATGACAAAACCAAAAAAAAAGATCATTTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

214

Amino Acids

24.91

Weight (kDa)

7.71

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 93 - 214 5.9e-30 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 315
AccI GTMKAC 1 cut(s) 387
AccIII TCCGGA 2 cut(s) 144, 529
AclI AACGTT 1 cut(s) 376
AclWI GGATC 3 cut(s) 89, 104, 136
AcyI GRCGYC 1 cut(s) 316
AfaI GTAC 2 cut(s) 206, 569
AgsI TTSAA 1 cut(s) 458
AluBI AGCT 4 cut(s) 45, 275, 361, 503
AluI AGCT 4 cut(s) 45, 275, 361, 503
AlwI GGATC 3 cut(s) 89, 104, 136
Aor13HI TCCGGA 2 cut(s) 144, 529
ApeKI GCWGC 1 cut(s) 500
AspLEI GCGC 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 555
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 2 cut(s) 94, 163
BarI GAAGNNNNNNTAC 2 cut(s) 581, 613
BauI CACGAG 1 cut(s) 162
BbvI GCAGC 1 cut(s) 512
BccI CCATC 3 cut(s) 251, 456, 586
BciVI GTATCC 1 cut(s) 485
BclI TGATCA 1 cut(s) 166
BfaI CTAG 2 cut(s) 272, 362
BfoI RGCGCY 1 cut(s) 319
BfuI GTATCC 1 cut(s) 485
BglI GCCNNNNNGGC 1 cut(s) 424
BglII AGATCT 1 cut(s) 451
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
BmiI GGNNCC 2 cut(s) 241, 317
BpuEI CTTGAG 2 cut(s) 185, 416
Bsa29I ATCGAT 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 120
BsaWI WCCGGW 2 cut(s) 144, 529
Bse1I ACTGG 2 cut(s) 33, 419
Bse3DI GCAATG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 525
BseAI TCCGGA 2 cut(s) 144, 529
BseCI ATCGAT 1 cut(s) 609
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 136, 467
BseJI GATNNNNATC 1 cut(s) 525
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 2 cut(s) 200, 261
BseNI ACTGG 2 cut(s) 33, 419
BseRI GAGGAG 2 cut(s) 106, 380
BseXI GCAGC 1 cut(s) 512
BshNI GGYRCC 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 609
BsiSI CCGG 2 cut(s) 145, 530
BslFI GGGAC 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 39
BsmI GAATGC 1 cut(s) 420
Bsp1286I GDGCHC 2 cut(s) 94, 163
Bsp13I TCCGGA 2 cut(s) 144, 529
Bsp143I GATC 6 cut(s) 81, 109, 141, 166, 451, 631
BspCNI CTCAG 2 cut(s) 201, 260
BspDI ATCGAT 1 cut(s) 609
BspEI TCCGGA 2 cut(s) 144, 529
BspHI TCATGA 4 cut(s) 84, 138, 169, 576
BspLI GGNNCC 2 cut(s) 241, 317
BspPI GGATC 3 cut(s) 89, 104, 136
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 315
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 2 cut(s) 33, 419
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 6 cut(s) 81, 109, 141, 166, 451, 631
BssNI GRCGYC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 120
Bst2BI CACGAG 1 cut(s) 162
Bst4CI ACNGT 1 cut(s) 567
Bst6I CTCTTC 2 cut(s) 27, 200
BstACI GRCGYC 1 cut(s) 316
BstDEI CTNAG 3 cut(s) 209, 247, 496
BstF5I GGATG 2 cut(s) 136, 467
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 6 cut(s) 84, 112, 144, 169, 454, 634
BstMBI GATC 6 cut(s) 81, 109, 141, 166, 451, 631
BstMWI GCNNNNNNNGC 3 cut(s) 51, 358, 424
BstV1I GCAGC 1 cut(s) 512
BstX2I RGATCY 2 cut(s) 109, 451
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 2 cut(s) 109, 451
Bsu15I ATCGAT 1 cut(s) 609
BsuI GTATCC 1 cut(s) 485
BsuTUI ATCGAT 1 cut(s) 609
BtsCI GGATG 2 cut(s) 136, 467
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 426
CciI TCATGA 4 cut(s) 84, 138, 169, 576
CfoI GCGC 1 cut(s) 318
ClaI ATCGAT 1 cut(s) 609
Csp6I GTAC 2 cut(s) 205, 568
CviAII CATG 4 cut(s) 85, 139, 170, 577
CviJI RGCY 9 cut(s) 45, 72, 92, 161, 185, 275, 361, 503, 538
CviKI_1 RGCY 9 cut(s) 45, 72, 92, 161, 185, 275, 361, 503, 538
CviQI GTAC 2 cut(s) 205, 568
DdeI CTNAG 3 cut(s) 209, 247, 496
DinI GGCGCC 1 cut(s) 317
DpnI GATC 6 cut(s) 83, 111, 143, 168, 453, 633
DpnII GATC 6 cut(s) 81, 109, 141, 166, 451, 631
DraI TTTAAA 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 27, 200
EarI CTCTTC 2 cut(s) 27, 200
Eco130I CCWWGG 1 cut(s) 120
Eco24I GRGCYC 2 cut(s) 94, 163
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 2 cut(s) 94, 163
EgeI GGCGCC 1 cut(s) 317
EheI GGCGCC 1 cut(s) 317
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 4 cut(s) 88, 142, 173, 580
FaqI GGGAC 1 cut(s) 39
FatI CATG 4 cut(s) 84, 138, 169, 576
FbaI TGATCA 1 cut(s) 166
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 2 cut(s) 143, 474
FriOI GRGCYC 2 cut(s) 94, 163
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 2 cut(s) 272, 362
GlaI GCGC 1 cut(s) 317
GluI GCNGC 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 319
HapII CCGG 2 cut(s) 145, 530
HhaI GCGC 1 cut(s) 318
Hin1I GRCGYC 1 cut(s) 316
Hin1II CATG 4 cut(s) 88, 142, 173, 580
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 3 cut(s) 5, 251, 259
HpaII CCGG 2 cut(s) 145, 530
HphI GGTGA 1 cut(s) 555
Hpy166II GTNNAC 3 cut(s) 304, 388, 598
Hpy188I TCNGA 2 cut(s) 250, 256
Hpy8I GTNNAC 3 cut(s) 304, 388, 598
HpyAV CCTTC 3 cut(s) 175, 304, 452
HpyCH4III ACNGT 1 cut(s) 567
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 2 cut(s) 295, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 358, 424
HpyF3I CTNAG 3 cut(s) 209, 247, 496
HpySE526I ACGT 1 cut(s) 376
Hsp92I GRCGYC 1 cut(s) 316
Hsp92II CATG 4 cut(s) 88, 142, 173, 580
HspAI GCGC 1 cut(s) 316
KasI GGCGCC 1 cut(s) 315
Kpn2I TCCGGA 2 cut(s) 144, 529
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 6 cut(s) 81, 109, 141, 166, 451, 631
LguI GCTCTTC 1 cut(s) 27
LpnPI CCDG 8 cut(s) 14, 158, 221, 413, 424, 432, 503, 543
Lsp1109I GCAGC 1 cut(s) 512
MaeI CTAG 2 cut(s) 272, 362
MaeII ACGT 1 cut(s) 376
MalI GATC 6 cut(s) 83, 111, 143, 168, 453, 633
MboI GATC 6 cut(s) 81, 109, 141, 166, 451, 631
MboII GAAGA 6 cut(s) 44, 119, 146, 187, 412, 601
MflI RGATCY 2 cut(s) 109, 451
MhlI GDGCHC 2 cut(s) 94, 163
Mly113I GGCGCC 1 cut(s) 316
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 194, 234
MnlI CCTC 9 cut(s) 16, 124, 127, 203, 209, 210, 336, 358, 528
MroI TCCGGA 2 cut(s) 144, 529
MseI TTAA 1 cut(s) 372
MspI CCGG 2 cut(s) 145, 530
MssI GTTTAAAC 1 cut(s) 373
Mva1269I GAATGC 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 51, 358, 424
NarI GGCGCC 1 cut(s) 316
NdeII GATC 6 cut(s) 81, 109, 141, 166, 451, 631
NlaIII CATG 4 cut(s) 88, 142, 173, 580
NlaIV GGNNCC 2 cut(s) 241, 317
PagI TCATGA 4 cut(s) 84, 138, 169, 576
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 5, 259
PkrI GCNGC 1 cut(s) 502
PleI GAGTC 1 cut(s) 259
PluTI GGCGCC 1 cut(s) 319
PmeI GTTTAAAC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 259
Psp1406I AACGTT 1 cut(s) 376
PspN4I GGNNCC 2 cut(s) 241, 317
PsuI RGATCY 2 cut(s) 109, 451
RsaI GTAC 2 cut(s) 206, 569
RsaNI GTAC 2 cut(s) 205, 568
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 6 cut(s) 81, 109, 141, 166, 451, 631
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 2 cut(s) 94, 163
SetI ASST 8 cut(s) 47, 195, 210, 277, 363, 379, 505, 573
SfoI GGCGCC 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 200, 395
SmoI CTYRAG 2 cut(s) 200, 395
SspDI GGCGCC 1 cut(s) 315
SspMI CTAG 2 cut(s) 272, 362
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 567
TaiI ACGT 1 cut(s) 379
TaqI TCGA 2 cut(s) 177, 609
TfiI GAWTC 2 cut(s) 5, 259
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 2 cut(s) 61, 426
TseI GCWGC 1 cut(s) 500
TspDTI ATGAA 2 cut(s) 127, 565
TspRI CASTG 2 cut(s) 61, 426
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 2 cut(s) 272, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.