Rorug04G0229300

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011976
Physical Location & Seq
Reverse (-)
39337292 .. 39337867
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0229300.1

Sequence Viewer

Length: 576 bp
ATGGCGGACCCCAACTTCTCCTTCCTCCTCCTCCTCCTCCACTTCATCGTCAGCCCTCGCCCCACCGCCGTCCCAATCAAGAACCGCCACGTCTTCATCACCGGCGGTTCCAGCAGCATCGGCCTCGCCCTGGCCCACCGCGCCGCCTCCGAGGGCGCCAAAATCTCCATCCTCGCCCGCTCCCTCAACAAGCTCGAAGAGGCCAAGAACTACATCCAGCTCTCCATCCGCATCCACGTCGCCGTCTTCGCCGCTGACGTCCGTGACTACGACGCAGTTTCGAAGGCTATCGAGGCGGCAGGCCCGATCCACGTGTTGGTCGTCAACCAGGGAGTGTTTATGGCCAGGGAGTTGGAGAAGCAGGTATTGGATGAGGTCAGGTTCATGGTGGACGTCAATCTGATCGGTAGCTTCAACATGATAAAAGCTGCTTTGCCGGGAATGAAAGACAGGAAGGACCGTGGGCCGGGCTCGACTTTTCTAATGTTGTCTCAGGCAGGTCAGGTTGGGATTTATCTCTATGCTGCTTATTCAGCTAGCAAATTTGGGCTTCAGGGTTTGACTGAAACTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

191

Amino Acids

20.73

Weight (kDa)

9.39

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 30 - 191 2.7e-33 short chain dehydrogenase
KR PF08659 31 - 181 4.1e-08 KR domain
adh_short_C2 PF13561 38 - 191 1.9e-19 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 261, 396
Acc36I ACCTGC 2 cut(s) 352, 488
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 316
AccBSI CCGCTC 1 cut(s) 180
AccII CGCG 1 cut(s) 141
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 1 cut(s) 536
AcvI CACGTG 1 cut(s) 313
AcyI GRCGYC 3 cut(s) 156, 258, 393
AfiI CCNNNNNNNGG 3 cut(s) 130, 316, 466
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 1 cut(s) 415
AjiI CACGTC 2 cut(s) 91, 238
AjnI CCWGG 3 cut(s) 129, 327, 344
AjuI GAANNNNNNNTTGG 3 cut(s) 37, 350, 382
AluBI AGCT 5 cut(s) 193, 220, 411, 428, 536
AluI AGCT 5 cut(s) 193, 220, 411, 428, 536
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 1 cut(s) 301
AoxI GGCC 6 cut(s) 121, 132, 201, 301, 342, 464
ApeKI GCWGC 3 cut(s) 114, 428, 524
ApoI RAATTY 1 cut(s) 542
AspLEI GCGC 2 cut(s) 143, 158
AspS9I GGNCC 5 cut(s) 7, 133, 302, 457, 464
AsuC2I CCSGG 2 cut(s) 438, 468
AsuHPI GGTGA 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 281
AsuNHI GCTAGC 1 cut(s) 536
AvaII GGWCC 2 cut(s) 7, 457
BalI TGGCCA 1 cut(s) 344
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 473
BbrPI CACGTG 1 cut(s) 313
BbsI GAAGAC 2 cut(s) 85, 238
BbvI GCAGC 3 cut(s) 126, 415, 511
BccI CCATC 2 cut(s) 176, 233
BceAI ACGGC 2 cut(s) 53, 227
BcgI CGANNNNNNTGC 4 cut(s) 106, 140, 220, 254
BciT130I CCWGG 3 cut(s) 131, 329, 346
BcnI CCSGG 2 cut(s) 438, 468
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 1 cut(s) 572
BfoI RGCGCY 1 cut(s) 159
BfuAI ACCTGC 2 cut(s) 352, 488
BisI GCNGC 6 cut(s) 115, 144, 252, 297, 429, 525
BlsI GCNGC 6 cut(s) 116, 145, 253, 298, 430, 526
Bme1390I CCNGG 5 cut(s) 131, 329, 346, 438, 468
Bme18I GGWCC 2 cut(s) 7, 457
BmgBI CACGTC 2 cut(s) 91, 238
BmgT120I GGNCC 5 cut(s) 7, 133, 302, 457, 464
BmiI GGNNCC 3 cut(s) 9, 109, 157
BmrFI CCNGG 5 cut(s) 131, 329, 346, 438, 468
BmsI GCATC 2 cut(s) 126, 240
BmtI GCTAGC 1 cut(s) 540
BpiI GAAGAC 2 cut(s) 85, 238
Bpu14I TTCGAA 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 438, 468
BsaAI YACGTR 1 cut(s) 313
BsaHI GRCGYC 3 cut(s) 156, 258, 393
BsaJI CCNNGG 5 cut(s) 129, 150, 328, 345, 460
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 316, 466
Bse118I RCCGGY 1 cut(s) 101
BseBI CCWGG 3 cut(s) 131, 329, 346
BseDI CCNNGG 5 cut(s) 129, 150, 328, 345, 460
BseGI GGATG 5 cut(s) 168, 213, 225, 231, 376
BseLI CCNNNNNNNGG 3 cut(s) 130, 316, 466
BseMII CTCAG 1 cut(s) 506
BseRI GAGGAG 4 cut(s) 17, 20, 23, 26
BseXI GCAGC 3 cut(s) 126, 415, 511
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 6 cut(s) 123, 134, 203, 303, 344, 466
BshNI GGYRCC 1 cut(s) 155
BsiSI CCGG 3 cut(s) 102, 437, 467
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 3 cut(s) 130, 316, 466
BsmAI GTCTC 1 cut(s) 495
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 6 cut(s) 123, 134, 203, 303, 344, 466
Bsp119I TTCGAA 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 2 cut(s) 306, 402
BspANI GGCC 6 cut(s) 123, 134, 203, 303, 344, 466
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 141
BspLI GGNNCC 3 cut(s) 9, 109, 157
BspMI ACCTGC 2 cut(s) 352, 488
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 1 cut(s) 301
BspT104I TTCGAA 1 cut(s) 281
BspT107I GGYRCC 1 cut(s) 155
BsrBI CCGCTC 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 5 cut(s) 129, 150, 328, 345, 460
BssMI GATC 2 cut(s) 306, 402
BssNI GRCGYC 3 cut(s) 156, 258, 393
Bst2UI CCWGG 3 cut(s) 131, 329, 346
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 1 cut(s) 192
BstACI GRCGYC 3 cut(s) 156, 258, 393
BstBAI YACGTR 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 281
BstC8I GCNNGC 3 cut(s) 178, 301, 538
BstDEI CTNAG 1 cut(s) 492
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 5 cut(s) 168, 213, 225, 231, 376
BstFNI CGCG 1 cut(s) 141
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 2 cut(s) 143, 158
BstKTI GATC 2 cut(s) 309, 405
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 2 cut(s) 306, 402
BstMWI GCNNNNNNNGC 6 cut(s) 111, 120, 140, 248, 293, 533
BstNI CCWGG 3 cut(s) 131, 329, 346
BstSCI CCNGG 5 cut(s) 129, 327, 344, 436, 466
BstSFI CTRYAG 1 cut(s) 572
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 3 cut(s) 126, 415, 511
BstV2I GAAGAC 2 cut(s) 85, 238
BstXI CCANNNNNNTGG 1 cut(s) 352
BsuRI GGCC 6 cut(s) 123, 134, 203, 303, 344, 466
BtgI CCRYGG 1 cut(s) 460
BtrI CACGTC 2 cut(s) 91, 238
BtsCI GGATG 5 cut(s) 168, 213, 225, 231, 376
BveI ACCTGC 2 cut(s) 352, 488
Cac8I GCNNGC 3 cut(s) 178, 301, 538
CfoI GCGC 2 cut(s) 143, 158
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 5 cut(s) 7, 133, 302, 457, 464
CseI GACGC 1 cut(s) 281
CviAII CATG 2 cut(s) 385, 418
DdeI CTNAG 1 cut(s) 492
DinI GGCGCC 1 cut(s) 157
DpnI GATC 2 cut(s) 308, 404
DpnII GATC 2 cut(s) 306, 402
EaeI YGGCCR 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 473
Eco47I GGWCC 2 cut(s) 7, 457
Eco57I CTGAAG 1 cut(s) 536
Eco72I CACGTG 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 129, 327, 344
EcoT38I GRGCYC 1 cut(s) 473
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
FaeI CATG 2 cut(s) 388, 421
FaiI YATR 5 cut(s) 341, 386, 419, 522, 574
FaqI GGGAC 1 cut(s) 56
FatI CATG 2 cut(s) 384, 417
FauI CCCGC 1 cut(s) 185
Fnu4HI GCNGC 6 cut(s) 115, 144, 252, 297, 429, 525
FokI GGATG 5 cut(s) 155, 200, 212, 218, 383
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 6 cut(s) 115, 144, 252, 297, 429, 525
FspBI CTAG 1 cut(s) 537
GlaI GCGC 2 cut(s) 142, 157
GluI GCNGC 6 cut(s) 115, 144, 252, 297, 429, 525
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 6 cut(s) 123, 134, 203, 303, 344, 466
HapII CCGG 3 cut(s) 102, 437, 467
HgaI GACGC 1 cut(s) 281
HhaI GCGC 2 cut(s) 143, 158
Hin1I GRCGYC 3 cut(s) 156, 258, 393
Hin1II CATG 2 cut(s) 388, 421
Hin6I GCGC 2 cut(s) 141, 156
HinP1I GCGC 2 cut(s) 141, 156
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HpaII CCGG 3 cut(s) 102, 437, 467
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 325, 391
Hpy188I TCNGA 2 cut(s) 151, 402
Hpy188III TCNNGA 1 cut(s) 79
Hpy8I GTNNAC 2 cut(s) 325, 391
Hpy99I CGWCG 2 cut(s) 242, 275
HpyAV CCTTC 3 cut(s) 31, 277, 448
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 5 cut(s) 90, 237, 258, 312, 393
HpyF10VI GCNNNNNNNGC 6 cut(s) 111, 120, 140, 248, 293, 533
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 5 cut(s) 90, 237, 258, 312, 393
Hsp92I GRCGYC 3 cut(s) 156, 258, 393
Hsp92II CATG 2 cut(s) 388, 421
HspAI GCGC 2 cut(s) 141, 156
KasI GGCGCC 1 cut(s) 155
Kzo9I GATC 2 cut(s) 306, 402
LmnI GCTCC 1 cut(s) 185
Lsp1109I GCAGC 3 cut(s) 126, 415, 511
LweI GCATC 2 cut(s) 126, 240
MaeI CTAG 1 cut(s) 537
MaeII ACGT 5 cut(s) 90, 237, 258, 312, 393
MaeIII GTNAC 1 cut(s) 263
MalI GATC 2 cut(s) 308, 404
MbiI CCGCTC 1 cut(s) 180
MboI GATC 2 cut(s) 306, 402
MboII GAAGA 3 cut(s) 85, 209, 238
MhlI GDGCHC 1 cut(s) 473
MlsI TGGCCA 1 cut(s) 344
MluCI AATT 1 cut(s) 542
MluNI TGGCCA 1 cut(s) 344
Mly113I GGCGCC 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 333
Mox20I TGGCCA 1 cut(s) 344
MscI TGGCCA 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 3 cut(s) 102, 437, 467
MspR9I CCNGG 5 cut(s) 131, 329, 346, 438, 468
MvaI CCWGG 3 cut(s) 131, 329, 346
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 6 cut(s) 111, 120, 140, 248, 293, 533
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 2 cut(s) 438, 468
NdeII GATC 2 cut(s) 306, 402
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 2 cut(s) 388, 421
NlaIV GGNNCC 3 cut(s) 9, 109, 157
NmuCI GTSAC 1 cut(s) 263
NspV TTCGAA 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 318
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 6 cut(s) 116, 145, 253, 298, 430, 526
PluTI GGCGCC 1 cut(s) 159
PmaCI CACGTG 1 cut(s) 313
PmlI CACGTG 1 cut(s) 313
Ppu21I YACGTR 1 cut(s) 313
Psp6I CCWGG 3 cut(s) 129, 327, 344
PspCI CACGTG 1 cut(s) 313
PspGI CCWGG 3 cut(s) 129, 327, 344
PspN4I GGNNCC 3 cut(s) 9, 109, 157
PspPI GGNCC 5 cut(s) 7, 133, 302, 457, 464
SatI GCNGC 6 cut(s) 115, 144, 252, 297, 429, 525
Sau3AI GATC 2 cut(s) 306, 402
Sau96I GGNCC 5 cut(s) 7, 133, 302, 457, 464
ScrFI CCNGG 5 cut(s) 131, 329, 346, 438, 468
SduI GDGCHC 1 cut(s) 473
SfaNI GCATC 2 cut(s) 126, 240
SfcI CTRYAG 1 cut(s) 572
SfoI GGCGCC 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 281
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 2 cut(s) 7, 457
Sse9I AATT 1 cut(s) 542
SspDI GGCGCC 1 cut(s) 155
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 5 cut(s) 129, 327, 344, 436, 466
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 5 cut(s) 93, 240, 261, 315, 396
TaqI TCGA 4 cut(s) 195, 281, 291, 473
TasI AATT 1 cut(s) 542
TauI GCSGC 3 cut(s) 146, 254, 299
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 3 cut(s) 114, 428, 524
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 4 cut(s) 34, 85, 373, 458
TspGWI ACGGA 1 cut(s) 251
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 7, 457
XapI RAATTY 1 cut(s) 542
XspI CTAG 1 cut(s) 537
ZraI GACGTC 2 cut(s) 259, 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.