pycom13g11450

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011976
Physical Location & Seq
Reverse (-)
7864020 .. 7865967
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g11450.2

Sequence Viewer

Length: 771 bp
ATGGAATCATCGAGCGGGAATGGGAAAGGCATGGCAGAGGCATATGAGGAAGTGGAAGAAGAAGCAAACTTTGAGAGCGATGAGATAGAGAGAAACAAAGTGGGAATCATGAGAGCACTTGTGGAAAGAGAAGATCCCTCCTCCAAGGATGTGGATGATTTCATGATTCGGAGATTTCTTCGGGCGCGTGATCTGGATATTGAGAAGGCTACTAACATGTTCCTCAAGTATCTGAGTTGGAAGAAGTCATTTATGCCGAATGGCTCCATCTCGGAGTCAGAGATTCCAAGCCAACTTGCTCAGAACAAGCTGTTCATGCAAGGCGTGGACAAGACTGGACGCCCCATTGTCGTTGTGTATGGTGGGCGGCATAAGCAGACCACACCTGAAGAGTTCAAACGTTTTGTAGTCTACACTCTTGATAAAATATGTTCCAGAATGCCAGCAGGAAAGGAAAAGTTTGCATGCATTGCGGATCTTGAAGGATGGGGCTATACCAATAGCGATGTTCGTGGATACATAGCAGCGCTGACGATCTTACAGGACAGCTACCCGGAGAGGCTTGGCAAGTTATTTTTTGTCCATGTGCCTTACATATTCATGACTGCTTGGAACATGGTTTACCCATTTATTGACAACAAAACCAAAAAGAAGATCCTTTTCGTCAAAGACAAAAAACTGAACTCAACCCTGCTGAAGGATATCGACGAGAGCCAGCTCCCAGATGCATATGGAGGCAGACTGCCATTAGTTCCCATCCAGAACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

257

Amino Acids

29.34

Weight (kDa)

6.24

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 411
AccII CGCG 1 cut(s) 187
AciI CCGC 3 cut(s) 15, 367, 473
AclI AACGTT 1 cut(s) 400
AclWI GGATC 3 cut(s) 128, 483, 649
AcuI CTGAAG 2 cut(s) 408, 716
AcyI GRCGYC 1 cut(s) 340
AfeI AGCGCT 1 cut(s) 528
AfiI CCNNNNNNNGG 1 cut(s) 697
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 2 cut(s) 397, 482
AluBI AGCT 3 cut(s) 310, 549, 718
AluI AGCT 3 cut(s) 310, 549, 718
Alw21I GWGCWC 1 cut(s) 118
AlwI GGATC 3 cut(s) 128, 483, 649
Aor51HI AGCGCT 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 524
AspLEI GCGC 2 cut(s) 187, 529
AsuC2I CCSGG 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 1 cut(s) 536
BccI CCATC 3 cut(s) 275, 480, 764
BciVI GTATCC 1 cut(s) 509
BcnI CCSGG 1 cut(s) 554
BfaI CTAG 1 cut(s) 769
BfoI RGCGCY 1 cut(s) 530
BfuI GTATCC 1 cut(s) 509
BisI GCNGC 2 cut(s) 368, 525
BlsI GCNGC 2 cut(s) 369, 526
Bme1390I CCNGG 1 cut(s) 554
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 554
BmsI GCATC 1 cut(s) 715
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 340
BsaJI CCNNGG 1 cut(s) 144
Bsc4I CCNNNNNNNGG 1 cut(s) 697
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 4 cut(s) 154, 160, 491, 756
BseLI CCNNNNNNNGG 1 cut(s) 697
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 2 cut(s) 224, 314
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 130
BseXI GCAGC 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 1 cut(s) 554
BslI CCNNNNNNNGG 1 cut(s) 697
BsmI GAATGC 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 5 cut(s) 133, 190, 475, 534, 654
BspACI CCGC 3 cut(s) 15, 367, 473
BspCNI CTCAG 2 cut(s) 225, 313
BspFNI CGCG 1 cut(s) 187
BspHI TCATGA 3 cut(s) 108, 162, 600
BspLI GGNNCC 1 cut(s) 265
BspPI GGATC 3 cut(s) 128, 483, 649
BsrBI CCGCTC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 5 cut(s) 133, 190, 475, 534, 654
BssNI GRCGYC 1 cut(s) 340
BssT1I CCWWGG 1 cut(s) 144
Bst6I CTCTTC 1 cut(s) 384
BstACI GRCGYC 1 cut(s) 340
BstAPI GCANNNNNTGC 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 444, 466, 716
BstDEI CTNAG 2 cut(s) 233, 300
BstENI CCTNNNNNAGG 1 cut(s) 695
BstF5I GGATG 4 cut(s) 154, 160, 491, 756
BstFNI CGCG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 2 cut(s) 187, 529
BstKTI GATC 5 cut(s) 136, 193, 478, 537, 657
BstMBI GATC 5 cut(s) 133, 190, 475, 534, 654
BstMWI GCNNNNNNNGC 3 cut(s) 316, 373, 470
BstNSI RCATGY 2 cut(s) 220, 468
BstSCI CCNGG 1 cut(s) 552
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 536
BstX2I RGATCY 3 cut(s) 133, 475, 654
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 3 cut(s) 133, 475, 654
BsuI GTATCC 1 cut(s) 509
BtgZI GCGATG 2 cut(s) 93, 519
BtsCI GGATG 4 cut(s) 154, 160, 491, 756
Cac8I GCNNGC 3 cut(s) 444, 466, 716
CciI TCATGA 3 cut(s) 108, 162, 600
CfoI GCGC 2 cut(s) 187, 529
CseI GACGC 1 cut(s) 348
CviAII CATG 9 cut(s) 31, 109, 163, 217, 316, 465, 584, 601, 616
CviJI RGCY 9 cut(s) 209, 264, 291, 310, 492, 549, 562, 714, 718
CviKI_1 RGCY 9 cut(s) 209, 264, 291, 310, 492, 549, 562, 714, 718
DdeI CTNAG 2 cut(s) 233, 300
DpnI GATC 5 cut(s) 135, 192, 477, 536, 656
DpnII GATC 5 cut(s) 133, 190, 475, 534, 654
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 144
Eco32I GATATC 1 cut(s) 703
Eco47III AGCGCT 1 cut(s) 528
Eco57I CTGAAG 2 cut(s) 408, 716
EcoNI CCTNNNNNAGG 1 cut(s) 695
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 2 cut(s) 470, 730
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 9 cut(s) 34, 112, 166, 220, 319, 468, 587, 604, 619
FatI CATG 9 cut(s) 30, 108, 162, 216, 315, 464, 583, 600, 615
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 2 cut(s) 43, 730
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 368, 525
FokI GGATG 4 cut(s) 161, 167, 498, 743
Fsp4HI GCNGC 2 cut(s) 368, 525
FspBI CTAG 1 cut(s) 769
GlaI GCGC 2 cut(s) 186, 528
GluI GCNGC 2 cut(s) 368, 525
HaeII RGCGCY 1 cut(s) 530
HapII CCGG 1 cut(s) 554
HgaI GACGC 1 cut(s) 348
HhaI GCGC 2 cut(s) 187, 529
Hin1I GRCGYC 1 cut(s) 340
Hin1II CATG 9 cut(s) 34, 112, 166, 220, 319, 468, 587, 604, 619
Hin6I GCGC 2 cut(s) 185, 527
HinP1I GCGC 2 cut(s) 185, 527
HinfI GANTC 5 cut(s) 5, 105, 166, 275, 283
HpaII CCGG 1 cut(s) 554
Hpy166II GTNNAC 3 cut(s) 328, 412, 622
Hpy188I TCNGA 5 cut(s) 171, 234, 274, 280, 303
Hpy188III TCNNGA 8 cut(s) 109, 163, 194, 419, 435, 479, 601, 760
Hpy8I GTNNAC 3 cut(s) 328, 412, 622
Hpy99I CGWCG 1 cut(s) 710
HpyAV CCTTC 3 cut(s) 199, 476, 691
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 4 cut(s) 319, 464, 468, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 373, 470
HpyF3I CTNAG 2 cut(s) 233, 300
HpySE526I ACGT 1 cut(s) 400
Hsp92I GRCGYC 1 cut(s) 340
Hsp92II CATG 9 cut(s) 34, 112, 166, 220, 319, 468, 587, 604, 619
HspAI GCGC 2 cut(s) 185, 527
Kzo9I GATC 5 cut(s) 133, 190, 475, 534, 654
LmnI GCTCC 2 cut(s) 269, 723
Lsp1109I GCAGC 1 cut(s) 536
LweI GCATC 1 cut(s) 715
MaeI CTAG 1 cut(s) 769
MaeII ACGT 1 cut(s) 400
MalI GATC 5 cut(s) 135, 192, 477, 536, 656
MbiI CCGCTC 1 cut(s) 15
MboI GATC 5 cut(s) 133, 190, 475, 534, 654
MboII GAAGA 7 cut(s) 68, 71, 143, 170, 253, 401, 664
MflI RGATCY 3 cut(s) 133, 475, 654
MhlI GDGCHC 1 cut(s) 118
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 7 cut(s) 31, 40, 148, 151, 233, 552, 728
Mph1103I ATGCAT 2 cut(s) 470, 730
MslI CAYNNNNRTG 1 cut(s) 599
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 1 cut(s) 554
Mva1269I GAATGC 1 cut(s) 444
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 3 cut(s) 316, 373, 470
NciI CCSGG 1 cut(s) 554
NdeI CATATG 2 cut(s) 43, 730
NdeII GATC 5 cut(s) 133, 190, 475, 534, 654
NlaIII CATG 9 cut(s) 34, 112, 166, 220, 319, 468, 587, 604, 619
NlaIV GGNNCC 1 cut(s) 265
NsiI ATGCAT 2 cut(s) 470, 730
NspI RCATGY 2 cut(s) 220, 468
PaeI GCATGC 1 cut(s) 468
PagI TCATGA 3 cut(s) 108, 162, 600
PciI ACATGT 1 cut(s) 216
PctI GAATGC 1 cut(s) 444
PfeI GAWTC 4 cut(s) 5, 105, 166, 283
PkrI GCNGC 2 cut(s) 369, 526
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PscI ACATGT 1 cut(s) 216
Psp1406I AACGTT 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 265
PsrI GAACNNNNNNTAC 2 cut(s) 605, 637
PsuI RGATCY 3 cut(s) 133, 475, 654
RseI CAYNNNNRTG 1 cut(s) 599
SatI GCNGC 2 cut(s) 368, 525
Sau3AI GATC 5 cut(s) 133, 190, 475, 534, 654
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 554
SduI GDGCHC 1 cut(s) 118
SetI ASST 5 cut(s) 312, 388, 403, 551, 720
SfaNI GCATC 1 cut(s) 715
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SphI GCATGC 1 cut(s) 468
SsiI CCGC 3 cut(s) 15, 367, 473
SspMI CTAG 1 cut(s) 769
StyD4I CCNGG 1 cut(s) 552
StyI CCWWGG 1 cut(s) 144
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 11, 705
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 4 cut(s) 5, 105, 166, 283
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 3 cut(s) 151, 304, 589
XagI CCTNNNNNAGG 1 cut(s) 695
XceI RCATGY 2 cut(s) 220, 468
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 1 cut(s) 769
Zsp2I ATGCAT 2 cut(s) 470, 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.