FvH4_4g22590

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011976
Physical Location & Seq
Forward (+)
25355951 .. 25359016
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22590.t1

Sequence Viewer

Length: 771 bp
ATGGGATCAAATGAGAAAGATAGTGTGATTGGTGTGGAAGCAAATGAGGGTGTGGAAGAGCAACAGGGTTTGGAAAGCAATGAGATTGAGAAGAGCAAAGTGGGTATCATGAGAGCCCTTGTGGAAAGAGAAGATCCCTCCTCCAAGGAAGTGGATGATTTCATGATCCGGAGATTTCTACGGGCTCGTGATCAGGACATCGAGAAGGCTTCTAACCTATTCCTCAAGTACCTGAGTTGGAGGAAAGCATTTATGCCAAATGGTTCAATCTCAGAGTCAGAGATTCCAAGAGAACTAGCACATAACAAGCTGTTTATGCAAGGTGTGGACAAGAAGGGGCGCCCCATTGTTGTTGTCTATGGTGGGAGGCATAAACACACGAAGCTAGAGGAGTTTAAACGGTTTGTAGTCTACACTCTTGAAAAGATATGTGCCAGAATGCCAGCAGGGCAGGAAAAGTTTTTATCCATTGCAGATCTCGAAGGATGGGGCTATGTCAATAGTGACGTTCGTGGATACTTAGCTGCTCTAACGATCTTACAGGATTGCTATCCGGAGAGGCTTGGCAAGTTATTCATCGTCCATGCACCTTACCTATTCATGACTGCTTGGAAGATGGTTTACCCGTTTATCGATGACAAAACCAAAAAAAAGATCATTTTTGTCGAGAACAAAAAGCTGAACTCCACATTACTTGGTGATATTGATGAGAGCCAGCTGCCTGATGCATACGGCGGAAAATTGCCATTAGTTCCAATCCAAGATTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

257

Amino Acids

29.27

Weight (kDa)

6.47

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 101 - 246 1.7e-38 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 113 - 245 8.9e-08 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 339
AccI GTMKAC 1 cut(s) 411
AccIII TCCGGA 2 cut(s) 168, 553
AciI CCGC 1 cut(s) 735
AclWI GGATC 3 cut(s) 13, 128, 160
AcyI GRCGYC 1 cut(s) 340
AfaI GTAC 1 cut(s) 230
AgsI TTSAA 2 cut(s) 267, 422
AluBI AGCT 5 cut(s) 310, 385, 524, 679, 718
AluI AGCT 5 cut(s) 310, 385, 524, 679, 718
AlwI GGATC 3 cut(s) 13, 128, 160
Aor13HI TCCGGA 2 cut(s) 168, 553
ApeKI GCWGC 2 cut(s) 524, 718
AspLEI GCGC 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 710
BanI GGYRCC 1 cut(s) 339
BanII GRGCYC 2 cut(s) 118, 187
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BauI CACGAG 1 cut(s) 186
BbvI GCAGC 2 cut(s) 511, 705
BccI CCATC 2 cut(s) 480, 610
BceAI ACGGC 1 cut(s) 748
BciVI GTATCC 1 cut(s) 509
BclI TGATCA 1 cut(s) 190
BfaI CTAG 2 cut(s) 296, 386
BfoI RGCGCY 1 cut(s) 343
BfuI GTATCC 1 cut(s) 509
BglI GCCNNNNNGGC 1 cut(s) 448
BglII AGATCT 1 cut(s) 475
BisI GCNGC 2 cut(s) 525, 719
BlsI GCNGC 2 cut(s) 526, 720
BmiI GGNNCC 1 cut(s) 341
BmsI GCATC 1 cut(s) 715
BpuEI CTTGAG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 633
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 340
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 2 cut(s) 168, 553
Bse3DI GCAATG 2 cut(s) 85, 468
Bse8I GATNNNNATC 1 cut(s) 549
BseAI TCCGGA 2 cut(s) 168, 553
BseCI ATCGAT 1 cut(s) 633
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 2 cut(s) 160, 491
BseJI GATNNNNATC 1 cut(s) 549
BseMI GCAATG 2 cut(s) 85, 468
BseMII CTCAG 2 cut(s) 224, 285
BseRI GAGGAG 2 cut(s) 130, 404
BseXI GCAGC 2 cut(s) 511, 705
BshNI GGYRCC 1 cut(s) 339
BshVI ATCGAT 1 cut(s) 633
BsiSI CCGG 2 cut(s) 169, 554
BsmI GAATGC 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 118, 187
Bsp13I TCCGGA 2 cut(s) 168, 553
Bsp143I GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
BspACI CCGC 1 cut(s) 735
BspCNI CTCAG 2 cut(s) 225, 284
BspDI ATCGAT 1 cut(s) 633
BspEI TCCGGA 2 cut(s) 168, 553
BspHI TCATGA 3 cut(s) 108, 162, 600
BspLI GGNNCC 1 cut(s) 341
BspPI GGATC 3 cut(s) 13, 128, 160
BspQI GCTCTTC 2 cut(s) 51, 86
BspT107I GGYRCC 1 cut(s) 339
BsrDI GCAATG 2 cut(s) 85, 468
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
BssNI GRCGYC 1 cut(s) 340
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 402
Bst6I CTCTTC 2 cut(s) 51, 86
BstACI GRCGYC 1 cut(s) 340
BstC8I GCNNGC 2 cut(s) 444, 716
BstDEI CTNAG 3 cut(s) 233, 271, 520
BstF5I GGATG 2 cut(s) 160, 491
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 7 cut(s) 8, 136, 168, 193, 478, 537, 657
BstMBI GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
BstMWI GCNNNNNNNGC 2 cut(s) 316, 448
BstV1I GCAGC 2 cut(s) 511, 705
BstX2I RGATCY 2 cut(s) 133, 475
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 2 cut(s) 133, 475
Bsu15I ATCGAT 1 cut(s) 633
BsuI GTATCC 1 cut(s) 509
BsuTUI ATCGAT 1 cut(s) 633
BtsCI GGATG 2 cut(s) 160, 491
Cac8I GCNNGC 2 cut(s) 444, 716
CciI TCATGA 3 cut(s) 108, 162, 600
CfoI GCGC 1 cut(s) 342
ClaI ATCGAT 1 cut(s) 633
Csp6I GTAC 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 676, 711
CviAII CATG 4 cut(s) 109, 163, 584, 601
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 3 cut(s) 233, 271, 520
DinI GGCGCC 1 cut(s) 341
DpnI GATC 7 cut(s) 7, 135, 167, 192, 477, 536, 656
DpnII GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
DraI TTTAAA 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 51, 86
EarI CTCTTC 2 cut(s) 51, 86
EciI GGCGGA 1 cut(s) 750
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 2 cut(s) 118, 187
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 730
EcoT38I GRGCYC 2 cut(s) 118, 187
EgeI GGCGCC 1 cut(s) 341
EheI GGCGCC 1 cut(s) 341
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 4 cut(s) 112, 166, 587, 604
FatI CATG 4 cut(s) 108, 162, 583, 600
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 525, 719
FokI GGATG 2 cut(s) 167, 498
FriOI GRGCYC 2 cut(s) 118, 187
Fsp4HI GCNGC 2 cut(s) 525, 719
FspBI CTAG 2 cut(s) 296, 386
GlaI GCGC 1 cut(s) 341
GluI GCNGC 2 cut(s) 525, 719
HaeII RGCGCY 1 cut(s) 343
HapII CCGG 2 cut(s) 169, 554
HhaI GCGC 1 cut(s) 342
Hin1I GRCGYC 1 cut(s) 340
Hin1II CATG 4 cut(s) 112, 166, 587, 604
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HinfI GANTC 2 cut(s) 275, 283
HpaII CCGG 2 cut(s) 169, 554
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 3 cut(s) 328, 412, 622
Hpy188I TCNGA 2 cut(s) 274, 280
Hpy8I GTNNAC 3 cut(s) 328, 412, 622
HpyAV CCTTC 3 cut(s) 199, 328, 476
HpyCH4III ACNGT 1 cut(s) 402
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 4 cut(s) 319, 473, 587, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 316, 448
HpyF3I CTNAG 3 cut(s) 233, 271, 520
HpySE526I ACGT 1 cut(s) 507
Hsp92I GRCGYC 1 cut(s) 340
Hsp92II CATG 4 cut(s) 112, 166, 587, 604
HspAI GCGC 1 cut(s) 340
KasI GGCGCC 1 cut(s) 339
Kpn2I TCCGGA 2 cut(s) 168, 553
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
LguI GCTCTTC 2 cut(s) 51, 86
Lsp1109I GCAGC 2 cut(s) 511, 705
LweI GCATC 1 cut(s) 715
MaeI CTAG 2 cut(s) 296, 386
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 1 cut(s) 503
MalI GATC 7 cut(s) 7, 135, 167, 192, 477, 536, 656
MboI GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
MboII GAAGA 4 cut(s) 68, 103, 143, 625
MflI RGATCY 2 cut(s) 133, 475
MhlI GDGCHC 2 cut(s) 118, 187
MluCI AATT 1 cut(s) 740
Mly113I GGCGCC 1 cut(s) 340
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 8 cut(s) 40, 148, 151, 233, 234, 360, 382, 552
Mph1103I ATGCAT 1 cut(s) 730
MroI TCCGGA 2 cut(s) 168, 553
MseI TTAA 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 718
MspI CCGG 2 cut(s) 169, 554
MssI GTTTAAAC 1 cut(s) 397
Mva1269I GAATGC 1 cut(s) 444
MwoI GCNNNNNNNGC 2 cut(s) 316, 448
NarI GGCGCC 1 cut(s) 340
NdeII GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
NlaIII CATG 4 cut(s) 112, 166, 587, 604
NlaIV GGNNCC 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 730
PagI TCATGA 3 cut(s) 108, 162, 600
PciSI GCTCTTC 2 cut(s) 51, 86
PctI GAATGC 1 cut(s) 444
PfeI GAWTC 1 cut(s) 283
PkrI GCNGC 2 cut(s) 526, 720
PleI GAGTC 1 cut(s) 283
PluTI GGCGCC 1 cut(s) 343
PmeI GTTTAAAC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 341
PsuI RGATCY 2 cut(s) 133, 475
PvuII CAGCTG 1 cut(s) 718
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SapI GCTCTTC 2 cut(s) 51, 86
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 2 cut(s) 525, 719
Sau3AI GATC 7 cut(s) 5, 133, 165, 190, 475, 534, 654
SchI GAGTC 1 cut(s) 284
SduI GDGCHC 2 cut(s) 118, 187
SfaNI GCATC 1 cut(s) 715
SfoI GGCGCC 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 1 cut(s) 740
SsiI CCGC 1 cut(s) 735
SspDI GGCGCC 1 cut(s) 339
SspMI CTAG 2 cut(s) 296, 386
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 402
TaiI ACGT 1 cut(s) 510
TaqI TCGA 4 cut(s) 201, 480, 633, 666
TasI AATT 1 cut(s) 740
TfiI GAWTC 1 cut(s) 283
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 2 cut(s) 524, 718
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 3 cut(s) 151, 565, 589
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 2 cut(s) 296, 386
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.