MD13G1131200.v1.1

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011976
Physical Location & Seq
Reverse (-)
9970389 .. 9972257
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1131200.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCAGAGGCATATGAGGAAGTGGAAGGAGAAGCAAACTTTGAGAGCGATGAGATAGAGAGAAACAAAGTGGGAATCATGAGAGCACTTGTGGAAAGAGAAGATCCCTCCTCCAAGGATGTGGATGATTTCATGATCCGGAGATTTCTTCGGGCGCGTGATCTGGATATTGAGAAGGCTACTAACATGTTCCTCAAGTATCTGAGTTGGAAGAAGTCATTTGTGCCGAATGGCTCCATCTCGGAGTCAGAGATTCCAAGCCAACTTGCTCAGAACAAGCTGTTCATGCAAGGCGTGGACAAGACTGGACGCCCCATTATCGTTGTGTATGGTGGGCGGCATAAGCAGACCACACCTGAAGAGCTCAAACGTTTTGTAGTATACACTCTTGATAAAATATGTTCCAGAATGCCAGCAGGAAAGGAAAAGTTTGTATCCATTGCAGATCTTGAAGGATGGGGCTATACCAATAGCGATGTTCGTGGATACATAGCAGCGCTGACGATCTTACAGGACTGCTACCCGGAGAGGCTTGGCAAGTTATTTCTTGTCCATGTGCCCTATGTATTCATGACTGCTTGGAAGATGGTTTACCCATTTATTGACAACAAAACCAAAAAGAAGATCCTTTTTGTTGAAAACAAAAAACTGAACTCAACCCTGCTGAAAGATATCGACGAGAGCCAGCTCCCAGATGCATATGGAGGCAGACTGCCATTAGTTCCCATCCAGAACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

247

Amino Acids

28.28

Weight (kDa)

6.77

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 91 - 236 4.2e-38 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 103 - 235 6.6e-09 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01630
fragaria_vesca FvH4_4g22590
malus_domestica MD13G1131200.v1.1 MD16G1141400.v1.1
prunus_persica Prupe.1G214400_v2.0.a1
pyrus_communis pycom13g11450 pycom16g11190
rosa_chinensis RchiOBHm_Chr4g0428831
rosa_laevigata RLG00000007122
rosa_multiflora Rmu_sc0008612.1_g000001
rosa_roxburghii Rroxscaffold_5G00370510
rosa_rugosa Rorug04G0229300 Rorug04G0229400 Rorug04G0229500
rosa_samantha Rh4AG286200 Rh4BG292000 Rh4CG308000 Rh4DG289200
rosa_wichuraiana Rw4G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 381
AccII CGCG 1 cut(s) 157
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 1 cut(s) 337
AclI AACGTT 1 cut(s) 370
AclWI GGATC 3 cut(s) 98, 130, 619
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 310
AfeI AGCGCT 1 cut(s) 498
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 2 cut(s) 452, 638
AluBI AGCT 3 cut(s) 280, 364, 688
AluI AGCT 3 cut(s) 280, 364, 688
Alw21I GWGCWC 2 cut(s) 88, 366
AlwI GGATC 3 cut(s) 98, 130, 619
Aor13HI TCCGGA 1 cut(s) 138
Aor51HI AGCGCT 1 cut(s) 498
ApeKI GCWGC 1 cut(s) 494
AspLEI GCGC 2 cut(s) 157, 499
AsuC2I CCSGG 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 561
BanII GRGCYC 1 cut(s) 366
BarI GAAGNNNNNNTAC 2 cut(s) 575, 607
Bbv12I GWGCWC 2 cut(s) 88, 366
BbvI GCAGC 1 cut(s) 506
BccI CCATC 4 cut(s) 245, 450, 580, 734
BciVI GTATCC 2 cut(s) 445, 479
BcnI CCSGG 1 cut(s) 524
BfaI CTAG 1 cut(s) 739
BfoI RGCGCY 1 cut(s) 500
BfuI GTATCC 2 cut(s) 445, 479
BglII AGATCT 1 cut(s) 445
BisI GCNGC 2 cut(s) 338, 495
BlsI GCNGC 2 cut(s) 339, 496
Bme1390I CCNGG 1 cut(s) 524
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 1 cut(s) 685
BpuEI CTTGAG 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 138
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 4 cut(s) 124, 130, 461, 726
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 2 cut(s) 194, 284
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 1 cut(s) 506
Bsh1236I CGCG 1 cut(s) 157
BsiHKAI GWGCWC 2 cut(s) 88, 366
BsiSI CCGG 2 cut(s) 139, 524
BsmI GAATGC 1 cut(s) 414
Bsp1286I GDGCHC 3 cut(s) 88, 366, 561
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 6 cut(s) 103, 135, 160, 445, 504, 624
BspACI CCGC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 195, 283
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 1 cut(s) 157
BspHI TCATGA 3 cut(s) 78, 132, 570
BspLI GGNNCC 1 cut(s) 235
BspPI GGATC 3 cut(s) 98, 130, 619
BspQI GCTCTTC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 6 cut(s) 103, 135, 160, 445, 504, 624
BssNAI GTATAC 1 cut(s) 382
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 114
Bst1107I GTATAC 1 cut(s) 382
Bst6I CTCTTC 1 cut(s) 354
BstACI GRCGYC 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 414, 686
BstDEI CTNAG 2 cut(s) 203, 270
BstF5I GGATG 4 cut(s) 124, 130, 461, 726
BstFNI CGCG 1 cut(s) 157
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 2 cut(s) 157, 499
BstKTI GATC 6 cut(s) 106, 138, 163, 448, 507, 627
BstMBI GATC 6 cut(s) 103, 135, 160, 445, 504, 624
BstMWI GCNNNNNNNGC 2 cut(s) 286, 343
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 506
BstX2I RGATCY 3 cut(s) 103, 445, 624
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 3 cut(s) 103, 445, 624
BstZ17I GTATAC 1 cut(s) 382
BsuI GTATCC 2 cut(s) 445, 479
BtgZI GCGATG 2 cut(s) 63, 489
BtsCI GGATG 4 cut(s) 124, 130, 461, 726
Cac8I GCNNGC 2 cut(s) 414, 686
CciI TCATGA 3 cut(s) 78, 132, 570
CfoI GCGC 2 cut(s) 157, 499
CseI GACGC 1 cut(s) 318
CviAII CATG 6 cut(s) 79, 133, 187, 286, 554, 571
CviJI RGCY 9 cut(s) 179, 234, 261, 280, 364, 462, 532, 684, 688
CviKI_1 RGCY 9 cut(s) 179, 234, 261, 280, 364, 462, 532, 684, 688
DdeI CTNAG 2 cut(s) 203, 270
DpnI GATC 6 cut(s) 105, 137, 162, 447, 506, 626
DpnII GATC 6 cut(s) 103, 135, 160, 445, 504, 624
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 673
Eco47III AGCGCT 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 364
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 6 cut(s) 82, 136, 190, 289, 557, 574
FatI CATG 6 cut(s) 78, 132, 186, 285, 553, 570
FauNDI CATATG 2 cut(s) 13, 700
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 2 cut(s) 338, 495
FokI GGATG 4 cut(s) 131, 137, 468, 713
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 2 cut(s) 338, 495
FspBI CTAG 1 cut(s) 739
GlaI GCGC 2 cut(s) 156, 498
GluI GCNGC 2 cut(s) 338, 495
HaeII RGCGCY 1 cut(s) 500
HapII CCGG 2 cut(s) 139, 524
HgaI GACGC 1 cut(s) 318
HhaI GCGC 2 cut(s) 157, 499
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 6 cut(s) 82, 136, 190, 289, 557, 574
Hin6I GCGC 2 cut(s) 155, 497
HinP1I GCGC 2 cut(s) 155, 497
HinfI GANTC 3 cut(s) 75, 245, 253
HpaII CCGG 2 cut(s) 139, 524
Hpy166II GTNNAC 3 cut(s) 298, 382, 592
Hpy188I TCNGA 4 cut(s) 204, 244, 250, 273
Hpy188III TCNNGA 9 cut(s) 79, 133, 139, 164, 389, 405, 449, 571, 730
Hpy8I GTNNAC 3 cut(s) 298, 382, 592
Hpy99I CGWCG 1 cut(s) 680
HpyAV CCTTC 3 cut(s) 20, 169, 446
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 3 cut(s) 289, 443, 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 343
HpyF3I CTNAG 2 cut(s) 203, 270
HpySE526I ACGT 1 cut(s) 370
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 6 cut(s) 82, 136, 190, 289, 557, 574
HspAI GCGC 2 cut(s) 155, 497
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 6 cut(s) 103, 135, 160, 445, 504, 624
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 239, 693
Lsp1109I GCAGC 1 cut(s) 506
LweI GCATC 1 cut(s) 685
MaeI CTAG 1 cut(s) 739
MaeII ACGT 1 cut(s) 370
MalI GATC 6 cut(s) 105, 137, 162, 447, 506, 626
MboI GATC 6 cut(s) 103, 135, 160, 445, 504, 624
MboII GAAGA 6 cut(s) 113, 140, 223, 371, 595, 634
MflI RGATCY 3 cut(s) 103, 445, 624
MhlI GDGCHC 3 cut(s) 88, 366, 561
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 188
MnlI CCTC 6 cut(s) 10, 118, 121, 203, 522, 698
Mph1103I ATGCAT 1 cut(s) 700
MroI TCCGGA 1 cut(s) 138
MspI CCGG 2 cut(s) 139, 524
MspR9I CCNGG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 414
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 2 cut(s) 286, 343
NciI CCSGG 1 cut(s) 524
NdeI CATATG 2 cut(s) 13, 700
NdeII GATC 6 cut(s) 103, 135, 160, 445, 504, 624
NlaIII CATG 6 cut(s) 82, 136, 190, 289, 557, 574
NlaIV GGNNCC 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 1 cut(s) 190
PagI TCATGA 3 cut(s) 78, 132, 570
PciI ACATGT 1 cut(s) 186
PciSI GCTCTTC 1 cut(s) 354
PctI GAATGC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 75, 253
PkrI GCNGC 2 cut(s) 339, 496
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PscI ACATGT 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 235
PsuI RGATCY 3 cut(s) 103, 445, 624
SacI GAGCTC 1 cut(s) 366
SapI GCTCTTC 1 cut(s) 354
SatI GCNGC 2 cut(s) 338, 495
Sau3AI GATC 6 cut(s) 103, 135, 160, 445, 504, 624
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 1 cut(s) 524
SduI GDGCHC 3 cut(s) 88, 366, 561
SetI ASST 5 cut(s) 282, 358, 366, 373, 690
SfaNI GCATC 1 cut(s) 685
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SsiI CCGC 1 cut(s) 337
SspMI CTAG 1 cut(s) 739
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 1 cut(s) 114
TaiI ACGT 1 cut(s) 373
TaqI TCGA 1 cut(s) 675
TauI GCSGC 1 cut(s) 340
TfiI GAWTC 2 cut(s) 75, 253
TseI GCWGC 1 cut(s) 494
TspDTI ATGAA 3 cut(s) 121, 274, 559
XceI RCATGY 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 1 cut(s) 739
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.