Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010853

Physical Location & Seq

Genomic Coordinates Forward (+)

775527 .. 780062

Gene Structure 4536 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G03190.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGATCTTTAAAATCGAAGACGTAACTGTCTACTTCCCTTATGACAACATATATCCAGAACAATATGAATACATGGTTGAATTGAAGCGAGCCCTAGACGCTAAGGGACATTGTCTTCTCGAGATGCCTACCGGAACTGGTAAAACCATTGCTCTTCTCTCTCTAATCACCAGCTATCGACTCTCTCGTCCTGATTCTCCGATCAAGCTTGTTTACTGTACTCGTACTGTCCACGAGATGGAGAAAACGTTAGGTGAGCTTAAGCTACTACATGACTACCAAGTACGCCATCTCGGCACCCAGGCTAAGATCCTCGCCCTTGGTCTTTCCTCTCGGAAGAATCTTTGCGTTAATACTAAGGTTTTAGCAGCCGAGAATCGTGATTCTGTTGATGCGGCCTGTAGGAAACGAACTGCTAGCTGGGTTAGGGCTTTGTCCACGGAGAATCCAAATGTAGAACTCTGTGACTTCTTTGAGAACTATGAAAAGGCTGCTGAGAATGCGTTGTTGCCTCCTGGTGTTTATACTTTAGAGGATCTAAGGGCGTTTGGTAAGAATCGTGGATGGTGCCCTTACTTTCTCGCGAGGCATATGATTCAGTTCGCCAACGTTATTGTTTACAGCTACCAATACCTACTGGATCCTAAGGTTGCTGGGTTCATATCAAAGGAGTTACAAAAAGAGTCAGTTGTAGTGTTTGACGAGGCCCATAATATCGATAACGTGTGTATTGAAGCACTCAGCGTCAGTGTGAGGAGGGTTACACTTGAAGGAGCTAATCGAAATCTTAATAAGATAAGGCAAGAAATTGATAGGTTCAAGGCTACTGATGCAGGAAGATTGCGAGCTGAATACAACCGGCTGGTTGAGGGTTTAGCACTGAGAGGGGACTTATCTGGAGGTGATCAATGGCTTGCAAACCCTGCACTGCCTCATGATATTCTAAAGGAGGCTGTCCCGGGGAATATTAGACGGGCTGAGCATTTTGTGCATGTTTTACGCCGATTACTTCAGTACTTGGGGGTACGGCTGGACACTGAGAATGTAGAGAAAGAAAGCCCTGTTAGCTTTGTATCGTCACTGAATTCTCAGGCTGGAATTGAGCAGAAAACACTGAAGTTCTGTTATGACCGGCTCCAGTCTCTCATGCTAACCCTTGAAATTACAGATACAGATGAGTTTTTGCCTATCCAGACAGTGTGCGACTTTGCAACTCTTGTTGGGACATATGCCCGGGGATTTTCCATCATCATCGAGCCTTATGACGAGAGAATGCCCCATATTCCAGATCCTATATTGCAGTTGAGCTGTCATGATGCGTCTCTGGCGATCAAACCGGTGTTTGATCGTTTCCAGTCAGTCGTGATTACATCAGGCACATTGAGCCCAATTGATCTTTACCCCCGTCTTCTTAATTTCACTCCTGTTGTTAGTCGAAGCTTTAAGATGTCAATGACACGAGACTGCATCTGCCCTATGGTTCTAACTCGGGGAAGTGATCAGCTTCCTGTTAGCACCAAATTTGACATGAGAAGTGATCCTGGTGTTGTGAGGAATTACGGAAAGCTTTTGGTAGAGATGGTATCTATTGTTCCTGATGGAGTTGTCTGCTTCTTTGTTAGTTACTCATATATGGATGGCATTATTGCTACATGGAATGAAACTGGAATTCTGAAGGAAATAATGCAACAGAAGCTTGTTTTCATTGAAACACAAGATGTTGTAGAAACAACATTGGCACTGGATAATTATCGCAGGGCTTGCGATTGCGGAAGAGGTGCAGTTTTCTTCTCTGTGGCCAGGGGGAAAGTTGCTGAAGGTATCGATTTTGATCGTCATTATGGAAGACTGGTTGTAATGTACGGAGTACCATTCCAGTATACATTAAGCAAGATATTACGAGCAAGATTGGAGTATTTGCACGATACATTTCAAATAAAAGAAGGCGATTTCCTAACTTTTGATGCCTTGAGGCAAGCAGCTCAATGTGTAGGGCGAGTAATCCGGTCAAAGGCTGATTATGGGATGATGATATTTGCAGACAAAAGATACAGCCGTCATGATAAGCGGTCCAAACTACCTGGTTGGATACTTTCGCATCTGCGTGATGCACACTTGAATCTAAGCACTGATATGGCTATTCACATTGCTCGAGAGTTTCTTCGGAAAATGGCTCAACCGTATGATAAGGCGGGTACAATGGGAAGGAAAACTCTGTTGACACAAGAAGATTTGGAGAAGATGGCTGAGACTGGTGTGCAAGACATGGCTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

DEAD_2 HBB Helicase_C_2

Protein Analysis

758

Amino Acids

86.23

Weight (kDa)

7.23

Isoelectric Point (pI)

45.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	72 - 255	5.8e-53	DEAD_2
HBB	PF06777	270 - 412	1.1e-37	Helical and beta-bridge domain
Helicase_C_2	PF13307	524 - 697	6.1e-38	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03190 AT1G03190
fragaria_vesca	FvH4_2g23690
malus_domestica	MD03G1266200.v1.1 MD11G1286800.v1.1
prunus_persica	Prupe.8G242000_v2.0.a1 Prupe.8G242000_v2.0.a1
pyrus_communis	pycom03g21270
rosa_chinensis	RchiOBHm_Chr6g0291151
rosa_laevigata	RLG00000012140
rosa_multiflora	Rmu_co8311277.1_g000001 Rmu_sc0004642.1_g000023 Rmu_sc0013275.1_g000002
rosa_roxburghii	Rroxscaffold_7G00176340
rosa_rugosa	Rorug06G0218000
rosa_samantha	Rh6AG328400 Rh6BG336000 Rh6CG342000 Rh6DG329300
rosa_wichuraiana	Rw6G028570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	26, 186
AccB1I	GGYRCC	2 cut(s)	296, 567
AccB7I	CCANNNNNTGG	1 cut(s)	238
AccI	GTMKAC	2 cut(s)	30, 1880
AccII	CGCG	1 cut(s)	584
AciI	CCGC	4 cut(s)	395, 1770, 2068, 2192
AclI	AACGTT	2 cut(s)	248, 609
AclWI	GGATC	6 cut(s)	304, 543, 635, 648, 1283, 1532
AcoI	YGGCCR	1 cut(s)	1797
AcsI	RAATTY	3 cut(s)	1084, 1520, 1668
AcuI	CTGAAG	4 cut(s)	995, 1136, 1694, 1836
AfaI	GTAC	8 cut(s)	220, 226, 285, 1016, 1026, 1862, 1869, 2197
AfiI	CCNNNNNNNGG	2 cut(s)	238, 2011
AflII	CTTAAG	1 cut(s)	260
AflIII	ACRYGT	1 cut(s)	723
AgeI	ACCGGT	1 cut(s)	1336
AgsI	TTSAA	9 cut(s)	80, 85, 734, 770, 820, 1160, 1709, 1934, 2119
AjnI	CCWGG	5 cut(s)	300, 514, 1540, 1799, 2080
Alw26I	GTCTC	4 cut(s)	1146, 1326, 1455, 2243
AlwI	GGATC	6 cut(s)	304, 543, 635, 648, 1283, 1532
Ama87I	CYCGRG	5 cut(s)	119, 958, 1233, 1488, 2151
AoxI	GGCC	3 cut(s)	396, 705, 1797
ApeKI	GCWGC	3 cut(s)	368, 491, 1979
ApoI	RAATTY	3 cut(s)	1084, 1520, 1668
AsiGI	ACCGGT	1 cut(s)	1336
AspS9I	GGNCC	2 cut(s)	706, 2070
AsuC2I	CCSGG	4 cut(s)	959, 960, 1234, 1235
AsuHPI	GGTGA	3 cut(s)	160, 266, 914
AsuNHI	GCTAGC	1 cut(s)	416
AvaI	CYCGRG	5 cut(s)	119, 958, 1233, 1488, 2151
AvaII	GGWCC	1 cut(s)	2070
AxyI	CCTNAGG	1 cut(s)	645
BaeGI	GKGCMC	1 cut(s)	572
BaeI	ACNNNNGTAYC	2 cut(s)	2041, 2074
BalI	TGGCCA	1 cut(s)	1799
BamHI	GGATCC	1 cut(s)	640
BanI	GGYRCC	2 cut(s)	296, 567
BanII	GRGCYC	2 cut(s)	94, 1388
BauI	CACGAG	2 cut(s)	233, 1458
BbsI	GAAGAC	4 cut(s)	24, 107, 1400, 1852
BbvI	GCAGC	3 cut(s)	380, 478, 1991
BccI	CCATC	8 cut(s)	232, 297, 558, 1253, 1573, 1592, 1631, 2236
BceAI	ACGGC	2 cut(s)	1043, 2040
BciT130I	CCWGG	5 cut(s)	302, 516, 1542, 1801, 2082
BciVI	GTATCC	1 cut(s)	2082
BclI	TGATCA	2 cut(s)	904, 1498
BcnI	CCSGG	4 cut(s)	959, 960, 1234, 1235
BcoDI	GTCTC	4 cut(s)	1146, 1326, 1455, 2243
BfaI	CTAG	2 cut(s)	95, 417
BfmI	CTRYAG	1 cut(s)	400
BfrI	CTTAAG	1 cut(s)	260
BfuI	GTATCC	1 cut(s)	2082
BglI	GCCNNNNNGGC	1 cut(s)	294
BisI	GCNGC	4 cut(s)	369, 396, 492, 1980
BlpI	GCTNAGC	1 cut(s)	978
BlsI	GCNGC	4 cut(s)	370, 397, 493, 1981
BmcAI	AGTACT	1 cut(s)	1016
Bme1390I	CCNGG	9 cut(s)	302, 516, 959, 960, 1234, 1235, 1542, 1801, 2082
Bme18I	GGWCC	1 cut(s)	2070
BmeT110I	CYCGRG	5 cut(s)	119, 958, 1233, 1488, 2151
BmgT120I	GGNCC	2 cut(s)	706, 2070
BmiI	GGNNCC	4 cut(s)	298, 569, 642, 1136
BmrFI	CCNGG	9 cut(s)	302, 516, 959, 960, 1234, 1235, 1542, 1801, 2082
BmsI	GCATC	8 cut(s)	114, 382, 820, 1306, 1476, 1954, 2098, 2107
BmtI	GCTAGC	1 cut(s)	420
BpiI	GAAGAC	4 cut(s)	24, 107, 1400, 1852
BpmI	CTGGAG	2 cut(s)	918, 1121
Bpu10I	CCTNAGC	1 cut(s)	102
Bpu1102I	GCTNAGC	1 cut(s)	978
BpuEI	CTTGAG	1 cut(s)	1990
BpuMI	CCSGG	4 cut(s)	959, 960, 1234, 1235
Bsa29I	ATCGAT	2 cut(s)	717, 1824
BsaBI	GATNNNNATC	2 cut(s)	1749, 1830
BsaJI	CCNNGG	8 cut(s)	300, 319, 438, 958, 959, 1233, 1234, 1800
BsaWI	WCCGGW	3 cut(s)	131, 1336, 2004
BsaXI	ACNNNNNCTCC	2 cut(s)	233, 263
Bsc4I	CCNNNNNNNGG	2 cut(s)	238, 2011
Bse118I	RCCGGY	3 cut(s)	858, 1131, 1336
Bse1I	ACTGG	9 cut(s)	142, 642, 1138, 1354, 1669, 1746, 1854, 1876, 2257
Bse21I	CCTNAGG	1 cut(s)	645
Bse3DI	GCAATG	2 cut(s)	147, 2145
Bse8I	GATNNNNATC	2 cut(s)	1749, 1830
BseBI	CCWGG	5 cut(s)	302, 516, 1542, 1801, 2082
BseCI	ATCGAT	2 cut(s)	717, 1824
BseDI	CCNNGG	8 cut(s)	300, 319, 438, 958, 959, 1233, 1234, 1800
BseGI	GGATG	3 cut(s)	569, 1642, 2031
BseJI	GATNNNNATC	2 cut(s)	1749, 1830
BseLI	CCNNNNNNNGG	2 cut(s)	238, 2011
BseMI	GCAATG	2 cut(s)	147, 2145
BseMII	CTCAG	7 cut(s)	486, 754, 872, 969, 1029, 1103, 2238
BseNI	ACTGG	9 cut(s)	142, 642, 1138, 1354, 1669, 1746, 1854, 1876, 2257
BseRI	GAGGAG	1 cut(s)	769
BseSI	GKGCMC	1 cut(s)	572
BseXI	GCAGC	3 cut(s)	380, 478, 1991
BseYI	CCCAGC	2 cut(s)	420, 653
BsgI	GTGCAG	2 cut(s)	909, 1800
Bsh1236I	CGCG	1 cut(s)	584
BshFI	GGCC	3 cut(s)	398, 707, 1799
BshNI	GGYRCC	2 cut(s)	296, 567
BshTI	ACCGGT	1 cut(s)	1336
BshVI	ATCGAT	2 cut(s)	717, 1824
BsiHKCI	CYCGRG	5 cut(s)	119, 958, 1233, 1488, 2151
BsiSI	CCGG	7 cut(s)	132, 859, 959, 1132, 1234, 1337, 2005
BslFI	GGGAC	4 cut(s)	120, 902, 941, 1237
BslI	CCNNNNNNNGG	2 cut(s)	238, 2011
BsmAI	GTCTC	4 cut(s)	1146, 1326, 1455, 2243
BsmBI	CGTCTC	1 cut(s)	1326
BsmFI	GGGAC	4 cut(s)	120, 902, 941, 1237
BsmI	GAATGC	2 cut(s)	505, 1278
BsnI	GGCC	3 cut(s)	398, 707, 1799
BsoBI	CYCGRG	5 cut(s)	119, 958, 1233, 1488, 2151
Bsp1286I	GDGCHC	3 cut(s)	94, 572, 1388
Bsp1720I	GCTNAGC	1 cut(s)	978
Bsp68I	TCGCGA	1 cut(s)	584
BspACI	CCGC	4 cut(s)	395, 1770, 2068, 2192
BspANI	GGCC	3 cut(s)	398, 707, 1799
BspCNI	CTCAG	7 cut(s)	487, 753, 873, 970, 1030, 1102, 2239
BspDI	ATCGAT	2 cut(s)	717, 1824
BspFNI	CGCG	1 cut(s)	584
BspHI	TCATGA	3 cut(s)	934, 1312, 2059
BspLI	GGNNCC	4 cut(s)	298, 569, 642, 1136
BspOI	GCTAGC	1 cut(s)	420
BspPI	GGATC	6 cut(s)	304, 543, 635, 648, 1283, 1532
BspQI	GCTCTTC	1 cut(s)	159
BspT107I	GGYRCC	2 cut(s)	296, 567
BspTI	CTTAAG	1 cut(s)	260
BsrDI	GCAATG	2 cut(s)	147, 2145
BsrFI	RCCGGY	3 cut(s)	858, 1131, 1336
BsrI	ACTGG	9 cut(s)	142, 642, 1138, 1354, 1669, 1746, 1854, 1876, 2257
BssAI	RCCGGY	3 cut(s)	858, 1131, 1336
BssECI	CCNNGG	8 cut(s)	300, 319, 438, 958, 959, 1233, 1234, 1800
BssNAI	GTATAC	1 cut(s)	1881
BssSI	CACGAG	2 cut(s)	233, 1458
BssT1I	CCWWGG	1 cut(s)	319
Bst1107I	GTATAC	1 cut(s)	1881
Bst2BI	CACGAG	2 cut(s)	233, 1458
Bst2UI	CCWGG	5 cut(s)	302, 516, 1542, 1801, 2082
Bst4CI	ACNGT	5 cut(s)	28, 218, 229, 1198, 2181
Bst6I	CTCTTC	2 cut(s)	159, 1768
BstAFI	CTTAAG	1 cut(s)	260
BstAPI	GCANNNNNTGC	3 cut(s)	923, 988, 1761
BstC8I	GCNNGC	6 cut(s)	90, 418, 846, 915, 1762, 1977
BstDSI	CCRYGG	1 cut(s)	438
BstF5I	GGATG	3 cut(s)	569, 1642, 2031
BstFNI	CGCG	1 cut(s)	584
BstMAI	GTCTC	4 cut(s)	1146, 1326, 1455, 2243
BstNI	CCWGG	5 cut(s)	302, 516, 1542, 1801, 2082
BstNSI	RCATGY	1 cut(s)	995
BstSCI	CCNGG	9 cut(s)	300, 514, 957, 958, 1232, 1233, 1540, 1799, 2080
BstSFI	CTRYAG	1 cut(s)	400
BstSLI	GKGCMC	1 cut(s)	572
BstUI	CGCG	1 cut(s)	584
BstV1I	GCAGC	3 cut(s)	380, 478, 1991
BstV2I	GAAGAC	4 cut(s)	24, 107, 1400, 1852
BstX2I	RGATCY	4 cut(s)	309, 535, 640, 1288
BstYI	RGATCY	4 cut(s)	309, 535, 640, 1288
BstZ17I	GTATAC	1 cut(s)	1881
Bsu15I	ATCGAT	2 cut(s)	717, 1824
Bsu36I	CCTNAGG	1 cut(s)	645
BsuI	GTATCC	1 cut(s)	2082
BsuRI	GGCC	3 cut(s)	398, 707, 1799
BsuTUI	ATCGAT	2 cut(s)	717, 1824
BtgI	CCRYGG	1 cut(s)	438
BtsCI	GGATG	3 cut(s)	569, 1642, 2031
BtsI	GCAGTG	1 cut(s)	926
BtsIMutI	CAGTG	9 cut(s)	754, 878, 926, 1035, 1079, 1112, 1203, 1739, 2127
BtuMI	TCGCGA	1 cut(s)	584
Cac8I	GCNNGC	6 cut(s)	90, 418, 846, 915, 1762, 1977
CciI	TCATGA	3 cut(s)	934, 1312, 2059
Cfr10I	RCCGGY	3 cut(s)	858, 1131, 1336
Cfr13I	GGNCC	2 cut(s)	706, 2070
Cfr9I	CCCGGG	2 cut(s)	958, 1233
ClaI	ATCGAT	2 cut(s)	717, 1824
CseI	GACGC	3 cut(s)	107, 733, 1308
CsiI	ACCWGGT	1 cut(s)	2080
Csp6I	GTAC	8 cut(s)	219, 225, 284, 1015, 1025, 1861, 1868, 2196
CspAI	ACCGGT	1 cut(s)	1336
CviQI	GTAC	8 cut(s)	219, 225, 284, 1015, 1025, 1861, 1868, 2196
DraI	TTTAAA	1 cut(s)	10
DrdI	GACNNNNNNGTC	2 cut(s)	26, 186
DseDI	GACNNNNNNGTC	2 cut(s)	26, 186
EaeI	YGGCCR	1 cut(s)	1797
Eam1104I	CTCTTC	2 cut(s)	159, 1768
EarI	CTCTTC	2 cut(s)	159, 1768
Eco130I	CCWWGG	1 cut(s)	319
Eco24I	GRGCYC	2 cut(s)	94, 1388
Eco47I	GGWCC	1 cut(s)	2070
Eco57I	CTGAAG	4 cut(s)	995, 1136, 1694, 1836
Eco81I	CCTNAGG	1 cut(s)	645
Eco88I	CYCGRG	5 cut(s)	119, 958, 1233, 1488, 2151
EcoRI	GAATTC	2 cut(s)	1084, 1668
EcoRII	CCWGG	5 cut(s)	300, 514, 1540, 1799, 2080
EcoT14I	CCWWGG	1 cut(s)	319
EcoT38I	GRGCYC	2 cut(s)	94, 1388
ErhI	CCWWGG	1 cut(s)	319
Esp3I	CGTCTC	1 cut(s)	1326
FaqI	GGGAC	4 cut(s)	120, 902, 941, 1237
FauI	CCCGC	1 cut(s)	2185
FauNDI	CATATG	2 cut(s)	591, 1228
FbaI	TGATCA	2 cut(s)	904, 1498
FblI	GTMKAC	2 cut(s)	30, 1880
Fnu4HI	GCNGC	4 cut(s)	369, 396, 492, 1980
FokI	GGATG	3 cut(s)	576, 1649, 2038
FriOI	GRGCYC	2 cut(s)	94, 1388
Fsp4HI	GCNGC	4 cut(s)	369, 396, 492, 1980
FspBI	CTAG	2 cut(s)	95, 417
GluI	GCNGC	4 cut(s)	369, 396, 492, 1980
GsaI	CCCAGC	2 cut(s)	424, 657
GsuI	CTGGAG	2 cut(s)	918, 1121
HaeIII	GGCC	3 cut(s)	398, 707, 1799
HapII	CCGG	7 cut(s)	132, 859, 959, 1132, 1234, 1337, 2005
HgaI	GACGC	3 cut(s)	107, 733, 1308
HincII	GTYRAC	1 cut(s)	2220
HindII	GTYRAC	1 cut(s)	2220
HindIII	AAGCTT	4 cut(s)	206, 1438, 1565, 1694
HpaII	CCGG	7 cut(s)	132, 859, 959, 1132, 1234, 1337, 2005
HphI	GGTGA	3 cut(s)	160, 266, 914
Hpy166II	GTNNAC	7 cut(s)	31, 214, 232, 438, 619, 1881, 2220
Hpy188I	TCNGA	4 cut(s)	201, 336, 1674, 2166
Hpy8I	GTNNAC	7 cut(s)	31, 214, 232, 438, 619, 1881, 2220
HpyAV	CCTTC	5 cut(s)	764, 1669, 1811, 1937, 2199
HpyCH4III	ACNGT	5 cut(s)	28, 218, 229, 1198, 2181
HpyCH4IV	ACGT	4 cut(s)	21, 248, 609, 723
HpySE526I	ACGT	4 cut(s)	21, 248, 609, 723
Ksp22I	TGATCA	2 cut(s)	904, 1498
LguI	GCTCTTC	1 cut(s)	159
LmnI	GCTCC	2 cut(s)	773, 1140
Lsp1109I	GCAGC	3 cut(s)	380, 478, 1991
LweI	GCATC	8 cut(s)	114, 382, 820, 1306, 1476, 1954, 2098, 2107
MabI	ACCWGGT	1 cut(s)	2080
MaeI	CTAG	2 cut(s)	95, 417
MaeII	ACGT	4 cut(s)	21, 248, 609, 723
MaeIII	GTNAC	6 cut(s)	22, 464, 672, 760, 1077, 1622
MfeI	CAATTG	1 cut(s)	1389
MflI	RGATCY	4 cut(s)	309, 535, 640, 1288
MhlI	GDGCHC	3 cut(s)	94, 572, 1388
MlsI	TGGCCA	1 cut(s)	1799
MluNI	TGGCCA	1 cut(s)	1799
MlyI	GAGTC	2 cut(s)	174, 692
MmeI	TCCRAC	1 cut(s)	2066
Mox20I	TGGCCA	1 cut(s)	1799
MscI	TGGCCA	1 cut(s)	1799
MseI	TTAA	7 cut(s)	9, 261, 351, 789, 1413, 1443, 1886
MslI	CAYNNNNRTG	2 cut(s)	2103, 2132
Msp20I	TGGCCA	1 cut(s)	1799
MspCI	CTTAAG	1 cut(s)	260
MspI	CCGG	7 cut(s)	132, 859, 959, 1132, 1234, 1337, 2005
MspR9I	CCNGG	9 cut(s)	302, 516, 959, 960, 1234, 1235, 1542, 1801, 2082
MunI	CAATTG	1 cut(s)	1389
Mva1269I	GAATGC	2 cut(s)	505, 1278
MvaI	CCWGG	5 cut(s)	302, 516, 1542, 1801, 2082
MvnI	CGCG	1 cut(s)	584
NciI	CCSGG	4 cut(s)	959, 960, 1234, 1235
NdeI	CATATG	2 cut(s)	591, 1228
NheI	GCTAGC	1 cut(s)	416
NlaIV	GGNNCC	4 cut(s)	298, 569, 642, 1136
NmeAIII	GCCGAG	2 cut(s)	273, 397
NmuCI	GTSAC	2 cut(s)	464, 1077
NruI	TCGCGA	1 cut(s)	584
NspI	RCATGY	1 cut(s)	995
PaeR7I	CTCGAG	2 cut(s)	119, 2151
PagI	TCATGA	3 cut(s)	934, 1312, 2059
PciSI	GCTCTTC	1 cut(s)	159
PcsI	WCGNNNNNNNCGW	1 cut(s)	184
PctI	GAATGC	2 cut(s)	505, 1278
PfeI	GAWTC	8 cut(s)	194, 340, 376, 383, 445, 556, 595, 2119
PflFI	GACNNNGTC	1 cut(s)	111
PflMI	CCANNNNNTGG	1 cut(s)	238
PinAI	ACCGGT	1 cut(s)	1336
PkrI	GCNGC	4 cut(s)	370, 397, 493, 1981
PleI	GAGTC	2 cut(s)	174, 691
PpsI	GAGTC	2 cut(s)	174, 691
Psp1406I	AACGTT	2 cut(s)	248, 609
Psp6I	CCWGG	5 cut(s)	300, 514, 1540, 1799, 2080
PspFI	CCCAGC	2 cut(s)	420, 653
PspGI	CCWGG	5 cut(s)	300, 514, 1540, 1799, 2080
PspN4I	GGNNCC	4 cut(s)	298, 569, 642, 1136
PspPI	GGNCC	2 cut(s)	706, 2070
PsrI	GAACNNNNNNTAC	2 cut(s)	1575, 1607
PsuI	RGATCY	4 cut(s)	309, 535, 640, 1288
PsyI	GACNNNGTC	1 cut(s)	111
RruI	TCGCGA	1 cut(s)	584
RsaI	GTAC	8 cut(s)	220, 226, 285, 1016, 1026, 1862, 1869, 2197
RsaNI	GTAC	8 cut(s)	219, 225, 284, 1015, 1025, 1861, 1868, 2196
RseI	CAYNNNNRTG	2 cut(s)	2103, 2132
SapI	GCTCTTC	1 cut(s)	159
SaqAI	TTAA	7 cut(s)	9, 261, 351, 789, 1413, 1443, 1886
SatI	GCNGC	4 cut(s)	369, 396, 492, 1980
Sau96I	GGNCC	2 cut(s)	706, 2070
ScaI	AGTACT	1 cut(s)	1016
SchI	GAGTC	2 cut(s)	174, 692
ScrFI	CCNGG	9 cut(s)	302, 516, 959, 960, 1234, 1235, 1542, 1801, 2082
SduI	GDGCHC	3 cut(s)	94, 572, 1388
SexAI	ACCWGGT	1 cut(s)	2080
SfaNI	GCATC	8 cut(s)	114, 382, 820, 1306, 1476, 1954, 2098, 2107
SfcI	CTRYAG	1 cut(s)	400
Sfr274I	CTCGAG	2 cut(s)	119, 2151
SinI	GGWCC	1 cut(s)	2070
SlaI	CTCGAG	2 cut(s)	119, 2151
SmaI	CCCGGG	2 cut(s)	960, 1235
SmiMI	CAYNNNNRTG	2 cut(s)	2103, 2132
SmlI	CTYRAG	4 cut(s)	119, 260, 1969, 2151
SmoI	CTYRAG	4 cut(s)	119, 260, 1969, 2151
SsiI	CCGC	4 cut(s)	395, 1770, 2068, 2192
SspI	AATATT	1 cut(s)	967
SspMI	CTAG	2 cut(s)	95, 417
StyD4I	CCNGG	9 cut(s)	300, 514, 957, 958, 1232, 1233, 1540, 1799, 2080
StyI	CCWWGG	1 cut(s)	319
TaaI	ACNGT	5 cut(s)	28, 218, 229, 1198, 2181
TaiI	ACGT	4 cut(s)	24, 251, 612, 726
TaqI	TCGA	9 cut(s)	15, 120, 178, 717, 781, 1254, 1435, 1824, 2152
TatI	WGTACW	2 cut(s)	218, 1014
TauI	GCSGC	1 cut(s)	398
TfiI	GAWTC	8 cut(s)	194, 340, 376, 383, 445, 556, 595, 2119
Tru1I	TTAA	7 cut(s)	9, 261, 351, 789, 1413, 1443, 1886
Tru9I	TTAA	7 cut(s)	9, 261, 351, 789, 1413, 1443, 1886
TscAI	CASTG	9 cut(s)	754, 885, 933, 1042, 1086, 1119, 1203, 1746, 2134
TseFI	GTSAC	2 cut(s)	464, 1077
TseI	GCWGC	3 cut(s)	368, 491, 1979
Tsp45I	GTSAC	2 cut(s)	464, 1077
TspDTI	ATGAA	5 cut(s)	81, 498, 649, 1674, 1693
TspGWI	ACGGA	3 cut(s)	455, 1575, 1878
TspMI	CCCGGG	2 cut(s)	958, 1233
TspRI	CASTG	9 cut(s)	754, 885, 933, 1042, 1086, 1119, 1203, 1746, 2134
Tth111I	GACNNNGTC	1 cut(s)	111
Van91I	CCANNNNNTGG	1 cut(s)	238
Vha464I	CTTAAG	1 cut(s)	260
VpaK11BI	GGWCC	1 cut(s)	2070
XapI	RAATTY	3 cut(s)	1084, 1520, 1668
XceI	RCATGY	1 cut(s)	995
XhoI	CTCGAG	2 cut(s)	119, 2151
XmaI	CCCGGG	2 cut(s)	958, 1233
XmiI	GTMKAC	2 cut(s)	30, 1880
XspI	CTAG	2 cut(s)	95, 417
ZrmI	AGTACT	1 cut(s)	1016

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.