Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0010853

Physical Location & Seq

Genomic Coordinates Reverse (-)

15804993 .. 15810223

Gene Structure 5231 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00176340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGAAGTTCCAAATGGACGACGTCACCGTGTACTTCCCCTACGACCACATCTACCCGGAGCAATACGCCTACATGCTGGAACTCAAACGCGCCCTGGACGCCCGAGGCCACTGCCTCCTCGAAATGCCCACCGGCACCGGAAAAACCATCGCCCTCCTCTCCCTCATCACCAGCTACACTCTCTCCAAGCCCTACAACCCCGTCAAGCTCATCTACTGCACCCGCACCGTCCACGAGATGGAGAAGACGCTCGCCGAGCTCAAGCTCCTCCATAAATACCAGGTCCAGCATCTCGGAAAACAGGCGGAGATCCTCGCCATCGGGCTCTCTAGTCGCAAGAACCTCTGCGTTAACCCTAATGTGCTTGCCGCCGAGAATCGCGACTCCGTGGACGCCGCTTGCCGCAAATTGACCGCCAGCTGGGTCCGCGCCGCCGCGATCGGGAACCCTAATGTGCCGAGCTGTGAGTTTTTTGAGCAGTTTGAGAGGGTCGGGGAAGGCACCGTCTTGCCTCCTGGGGTTTACACCTTGCAGGATTTGAGGGCGTTTGGGAAGAACAAAGGGTGGTGCCCTTACTTTTTGGCGAGGAGAATGGTGGAGGCGGCTAATGTGGTGGTTTATAGTTACCAGTATTTGCTTGATCCCAAAGTGGCTGGGATTATATCCAAGGAGTTGCAGAAGGAGTCGGTTGTGGTGTTCGATGAGGCTCACAATATAGACAATGTGTGTATCGAGGCGCTTAGTGTTAGTGTGAGGAGGCAGACCCTTGAGGGGGCTAGAAGGAATCTCAGTAAGATGCAGCAGGAAATTGAAAGGTTCAAGGCCACAGACGCAGGAAGATTGCGACAAGAATACAACCGGCTTGTTGAGGGTTTGGCACAAGGAGGAAACCTACCCATCACGGATGCTTGGCTTCAGAACCCTGCCCTACCTGACGATATTCTGAAGGAGGCTGTGCCCGGAAATATTCGCAAAGCGGACCATTTTCTGCATGTCTTACGAAGATTGGTCCAGTATCTCGAAGGGCGTCTTGAAACCGAGAACGTTGAGAAGGAGCTTCCTGTTGGCTTTGTTAACTCTATTAATACCCAAGCTGGAATTGACCAGAAAACTCTCAAGTTCTGCTATGATCGTCTACACTCACTCATGATGACATTAGAAATTACCGACACAGACGAGTTCTTACACATCCAAACTATATGCGACTTTGCGACGCTTGTGGGAACATATGCTCGTGGTTTCTCTATCATAATTGAGCCATTTGACGAGAGAATGCCACACATTCCAGATCCTGTTTTGCAGCTTAGTTGTCATGATGCTTCTCTTGCTATAAAGCCTGTATTTGATCGATTTCAATCTGTTGTGATTACATCTGGAACCTTGAGCCCGATTGATCTCTACCCCCGTCTTCTCAATTTTAACCCTGTTGTGAGTCGAAGCTTTACAATGTCACTAACGAGAGAATGCATATGCCCAATGGTTCTCACCCGGGGAAGTGATCAGCTTCCTGTAAGTACGAAATTTGATATGAGAAGTGATCTTGGAGTGGTAAGGAATTATGGGAGGCTGCTGCTGGAGATGGTTTCTGTTGTTCCAGATGGGGTCGAAATAATGCAGCATAAGCTGGTGTTCATTGAGACCCAAGATGTGGTAGAGACAACTTTGGCTTTGGACAACTACCGCAAAGCTTGCGATTGTGGGAGAGGTGCTGTCTTCTTTTCTGTAGCCAGGGGTAAAGTAGCTGAAGGAATAGACTTTGATCGTCATTATGGACGATTGGTCATCATGTACGGTGTTCCATTTCAGTACACGTTAAGCAAGATTTTGCTTGCACGGCTTGAGTACTTGAGGGAGACCTTTCAGATAAAGGAAGGAGATTTCTTGACCTTTGACGCTTTGAGACAAGCTGCCCAATGTGTCGGCCGGGTTATCCGGTCAAAAGCGGACTATGGCATGATGATTTTCGCGGATAAAAGGTATAGCCGTCATGACAAGCGGTCTAAGCTGCCAGGATGGATCCTCTCCCATCTGAGGGATGCGCATCTCAACTTGAGCACTGATATGGCTCTCCATATAGCACGAGAGTTCCTCAGAAAGATGGCCCAGCCATATGACAGGACTGGTGGCAGCAGCGGCAGGAAGACACTCCTATCCCAGGACGACTTGGAGAGGATGGGCCAAGGAATCACTTATGAAATGTTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

DEAD_2 HBB Helicase_C_2

Protein Analysis

735

Amino Acids

83.49

Weight (kDa)

7.55

Isoelectric Point (pI)

45.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	72 - 256	1.3e-52	DEAD_2
HBB	PF06777	270 - 412	2.8e-40	Helical and beta-bridge domain
Helicase_C_2	PF13307	540 - 673	6.1e-29	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03190 AT1G03190
fragaria_vesca	FvH4_2g23690
malus_domestica	MD03G1266200.v1.1 MD11G1286800.v1.1
prunus_persica	Prupe.8G242000_v2.0.a1 Prupe.8G242000_v2.0.a1
pyrus_communis	pycom03g21270
rosa_chinensis	RchiOBHm_Chr6g0291151
rosa_laevigata	RLG00000012140
rosa_multiflora	Rmu_co8311277.1_g000001 Rmu_sc0004642.1_g000023 Rmu_sc0013275.1_g000002
rosa_roxburghii	Rroxscaffold_7G00176340
rosa_rugosa	Rorug06G0218000
rosa_samantha	Rh6AG328400 Rh6BG336000 Rh6CG342000 Rh6DG329300
rosa_wichuraiana	Rw6G028570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	24
Acc16I	TGCGCA	1 cut(s)	2040
AccB1I	GGYRCC	3 cut(s)	134, 500, 567
AccB7I	CCANNNNNTGG	2 cut(s)	238, 1648
AccI	GTMKAC	1 cut(s)	1135
AccII	CGCG	5 cut(s)	90, 381, 429, 437, 1967
AclI	AACGTT	1 cut(s)	1044
AclWI	GGATC	5 cut(s)	304, 635, 1283, 2011, 2024
AcoI	YGGCCR	1 cut(s)	1921
AcsI	RAATTY	1 cut(s)	1520
AcuI	CTGAAG	3 cut(s)	899, 965, 1764
AcyI	GRCGYC	4 cut(s)	21, 99, 393, 1027
AfaI	GTAC	5 cut(s)	32, 1516, 1790, 1808, 1844
AfiI	CCNNNNNNNGG	9 cut(s)	238, 420, 441, 771, 772, 1648, 2031, 2032, 2155
AflIII	ACRYGT	1 cut(s)	1809
AgsI	TTSAA	4 cut(s)	812, 820, 1034, 1355
AhdI	GACNNNNNGTC	2 cut(s)	1778, 1996
AjnI	CCWGG	6 cut(s)	93, 279, 514, 1727, 2008, 2154
Alw21I	GWGCWC	2 cut(s)	261, 2057
Alw26I	GTCTC	4 cut(s)	1631, 1649, 1847, 1894
AlwI	GGATC	5 cut(s)	304, 635, 1283, 2011, 2024
AlwNI	CAGNNNCTG	1 cut(s)	1292
Ama87I	CYCGRG	2 cut(s)	102, 1488
AoxI	GGCC	5 cut(s)	106, 822, 1921, 2100, 2176
ApeKI	GCWGC	9 cut(s)	799, 1300, 1567, 1570, 1615, 1907, 2005, 2127, 2130
ApoI	RAATTY	1 cut(s)	1520
AseI	ATTAAT	1 cut(s)	1083
AspLEI	GCGC	4 cut(s)	92, 431, 739, 2041
AspS9I	GGNCC	6 cut(s)	283, 424, 979, 1009, 2101, 2176
AsuC2I	CCSGG	5 cut(s)	56, 960, 1489, 1490, 1925
AsuHPI	GGTGA	3 cut(s)	16, 160, 1477
AvaI	CYCGRG	2 cut(s)	102, 1488
AvaII	GGWCC	4 cut(s)	283, 424, 979, 1009
BaeGI	GKGCMC	2 cut(s)	572, 960
BamHI	GGATCC	1 cut(s)	2016
BanI	GGYRCC	3 cut(s)	134, 500, 567
BanII	GRGCYC	3 cut(s)	261, 327, 1388
BauI	CACGAG	3 cut(s)	233, 1233, 2079
BbsI	GAAGAC	4 cut(s)	251, 1400, 1705, 2147
Bbv12I	GWGCWC	2 cut(s)	261, 2057
BbvI	GCAGC	9 cut(s)	811, 1312, 1554, 1557, 1627, 1894, 1992, 2139, 2142
BceAI	ACGGC	2 cut(s)	1850, 1968
BcgI	CGANNNNNNTGC	2 cut(s)	93, 127
BciT130I	CCWGG	6 cut(s)	95, 281, 516, 1729, 2010, 2156
BclI	TGATCA	1 cut(s)	1498
BcnI	CCSGG	5 cut(s)	56, 960, 1489, 1490, 1925
BcoDI	GTCTC	4 cut(s)	1631, 1649, 1847, 1894
BfaI	CTAG	2 cut(s)	330, 777
BfmI	CTRYAG	1 cut(s)	1722
BfoI	RGCGCY	1 cut(s)	740
BmcAI	AGTACT	1 cut(s)	1844
Bme18I	GGWCC	4 cut(s)	283, 424, 979, 1009
BmeRI	GACNNNNNGTC	2 cut(s)	1778, 1996
BmeT110I	CYCGRG	2 cut(s)	102, 1488
BmgT120I	GGNCC	6 cut(s)	283, 424, 979, 1009, 2101, 2176
BmiI	GGNNCC	7 cut(s)	136, 425, 446, 502, 569, 1378, 2018
BmsI	GCATC	6 cut(s)	298, 786, 895, 1306, 2026, 2050
BpiI	GAAGAC	4 cut(s)	251, 1400, 1705, 2147
BplI	GAGNNNNNCTC	2 cut(s)	2073, 2105
BpmI	CTGGAG	1 cut(s)	1595
BpuEI	CTTGAG	7 cut(s)	245, 788, 1100, 1402, 1859, 1867, 2071
BpuMI	CCSGG	5 cut(s)	56, 960, 1489, 1490, 1925
Bsa29I	ATCGAT	1 cut(s)	1348
BsaBI	GATNNNNATC	2 cut(s)	1354, 2040
BsaHI	GRCGYC	4 cut(s)	21, 99, 393, 1027
BsaI	GGTCTC	2 cut(s)	1631, 1847
BsaWI	WCCGGW	2 cut(s)	137, 1932
BsaXI	ACNNNNNCTCC	2 cut(s)	167, 197
Bsc4I	CCNNNNNNNGG	9 cut(s)	238, 420, 441, 771, 772, 1648, 2031, 2032, 2155
Bse118I	RCCGGY	2 cut(s)	131, 858
Bse1I	ACTGG	3 cut(s)	628, 1012, 2125
Bse8I	GATNNNNATC	2 cut(s)	1354, 2040
BseBI	CCWGG	6 cut(s)	95, 281, 516, 1729, 2010, 2156
BseCI	ATCGAT	1 cut(s)	1348
BseGI	GGATG	5 cut(s)	910, 1188, 2018, 2041, 2178
BseJI	GATNNNNATC	2 cut(s)	1354, 2040
BseLI	CCNNNNNNNGG	9 cut(s)	238, 420, 441, 771, 772, 1648, 2031, 2032, 2155
BseMII	CTCAG	3 cut(s)	802, 2021, 2104
BseNI	ACTGG	3 cut(s)	628, 1012, 2125
BseRI	GAGGAG	5 cut(s)	107, 146, 257, 601, 769
BseSI	GKGCMC	2 cut(s)	572, 960
BseX3I	CGGCCG	1 cut(s)	1921
BseXI	GCAGC	9 cut(s)	811, 1312, 1554, 1557, 1627, 1894, 1992, 2139, 2142
BseYI	CCCAGC	3 cut(s)	420, 653, 2103
BsgI	GTGCAG	1 cut(s)	202
Bsh1236I	CGCG	5 cut(s)	90, 381, 429, 437, 1967
Bsh1285I	CGRYCG	2 cut(s)	441, 1924
BshFI	GGCC	5 cut(s)	108, 824, 1923, 2102, 2178
BshNI	GGYRCC	3 cut(s)	134, 500, 567
BshVI	ATCGAT	1 cut(s)	1348
BsiEI	CGRYCG	2 cut(s)	441, 1924
BsiHKAI	GWGCWC	2 cut(s)	261, 2057
BsiHKCI	CYCGRG	2 cut(s)	102, 1488
BsiSI	CCGG	8 cut(s)	56, 132, 138, 859, 960, 1489, 1924, 1933
BslI	CCNNNNNNNGG	9 cut(s)	238, 420, 441, 771, 772, 1648, 2031, 2032, 2155
BsmAI	GTCTC	4 cut(s)	1631, 1649, 1847, 1894
BsmI	GAATGC	2 cut(s)	1278, 1469
BsnI	GGCC	5 cut(s)	108, 824, 1923, 2102, 2178
Bso31I	GGTCTC	2 cut(s)	1631, 1847
BsoBI	CYCGRG	2 cut(s)	102, 1488
Bsp1286I	GDGCHC	6 cut(s)	261, 327, 572, 960, 1388, 2057
Bsp68I	TCGCGA	1 cut(s)	381
BspANI	GGCC	5 cut(s)	108, 824, 1923, 2102, 2178
BspCNI	CTCAG	3 cut(s)	801, 2022, 2103
BspDI	ATCGAT	1 cut(s)	1348
BspFNI	CGCG	5 cut(s)	90, 381, 429, 437, 1967
BspHI	TCATGA	3 cut(s)	1146, 1312, 1987
BspLI	GGNNCC	7 cut(s)	136, 425, 446, 502, 569, 1378, 2018
BspPI	GGATC	5 cut(s)	304, 635, 1283, 2011, 2024
BspT107I	GGYRCC	3 cut(s)	134, 500, 567
BspTNI	GGTCTC	2 cut(s)	1631, 1847
BsrFI	RCCGGY	2 cut(s)	131, 858
BsrI	ACTGG	3 cut(s)	628, 1012, 2125
BssAI	RCCGGY	2 cut(s)	131, 858
BssNI	GRCGYC	4 cut(s)	21, 99, 393, 1027
BssSI	CACGAG	3 cut(s)	233, 1233, 2079
BssT1I	CCWWGG	2 cut(s)	666, 2179
Bst2BI	CACGAG	3 cut(s)	233, 1233, 2079
Bst2UI	CCWGG	6 cut(s)	95, 281, 516, 1729, 2010, 2156
Bst4CI	ACNGT	4 cut(s)	28, 229, 505, 1793
BstACI	GRCGYC	4 cut(s)	21, 99, 393, 1027
BstAPI	GCANNNNNTGC	1 cut(s)	1689
BstC8I	GCNNGC	6 cut(s)	252, 366, 400, 418, 1690, 1830
BstDEI	CTNAG	6 cut(s)	740, 788, 1304, 2001, 2030, 2090
BstDSI	CCRYGG	1 cut(s)	387
BstENI	CCTNNNNNAGG	1 cut(s)	2153
BstF5I	GGATG	5 cut(s)	910, 1188, 2018, 2041, 2178
BstFNI	CGCG	5 cut(s)	90, 381, 429, 437, 1967
BstH2I	RGCGCY	1 cut(s)	740
BstHHI	GCGC	4 cut(s)	92, 431, 739, 2041
BstMAI	GTCTC	4 cut(s)	1631, 1649, 1847, 1894
BstMCI	CGRYCG	2 cut(s)	441, 1924
BstNI	CCWGG	6 cut(s)	95, 281, 516, 1729, 2010, 2156
BstNSI	RCATGY	2 cut(s)	76, 995
BstSFI	CTRYAG	1 cut(s)	1722
BstSLI	GKGCMC	2 cut(s)	572, 960
BstUI	CGCG	5 cut(s)	90, 381, 429, 437, 1967
BstV1I	GCAGC	9 cut(s)	811, 1312, 1554, 1557, 1627, 1894, 1992, 2139, 2142
BstV2I	GAAGAC	4 cut(s)	251, 1400, 1705, 2147
BstX2I	RGATCY	3 cut(s)	309, 1288, 2016
BstYI	RGATCY	3 cut(s)	309, 1288, 2016
BstZI	CGGCCG	1 cut(s)	1921
Bsu15I	ATCGAT	1 cut(s)	1348
BsuRI	GGCC	5 cut(s)	108, 824, 1923, 2102, 2178
BsuTUI	ATCGAT	1 cut(s)	1348
BtgI	CCRYGG	1 cut(s)	387
BtgZI	GCGATG	1 cut(s)	133
BtsCI	GGATG	5 cut(s)	910, 1188, 2018, 2041, 2178
BtsI	GCAGTG	1 cut(s)	109
BtsIMutI	CAGTG	2 cut(s)	109, 2055
BtuMI	TCGCGA	1 cut(s)	381
Cac8I	GCNNGC	6 cut(s)	252, 366, 400, 418, 1690, 1830
CaiI	CAGNNNCTG	1 cut(s)	1292
CciI	TCATGA	3 cut(s)	1146, 1312, 1987
CfoI	GCGC	4 cut(s)	92, 431, 739, 2041
Cfr10I	RCCGGY	2 cut(s)	131, 858
Cfr13I	GGNCC	6 cut(s)	283, 424, 979, 1009, 2101, 2176
Cfr9I	CCCGGG	1 cut(s)	1488
ClaI	ATCGAT	1 cut(s)	1348
CseI	GACGC	7 cut(s)	107, 256, 401, 839, 1016, 1222, 1901
CsiI	ACCWGGT	1 cut(s)	279
Csp6I	GTAC	5 cut(s)	31, 1515, 1789, 1807, 1843
CviAII	CATG	7 cut(s)	73, 992, 1147, 1313, 1786, 1954, 1988
CviQI	GTAC	5 cut(s)	31, 1515, 1789, 1807, 1843
DdeI	CTNAG	6 cut(s)	740, 788, 1304, 2001, 2030, 2090
DriI	GACNNNNNGTC	2 cut(s)	1778, 1996
EaeI	YGGCCR	1 cut(s)	1921
EagI	CGGCCG	1 cut(s)	1921
Eam1105I	GACNNNNNGTC	2 cut(s)	1778, 1996
EciI	GGCGGA	1 cut(s)	320
Ecl136II	GAGCTC	1 cut(s)	259
EclXI	CGGCCG	1 cut(s)	1921
Eco130I	CCWWGG	2 cut(s)	666, 2179
Eco24I	GRGCYC	3 cut(s)	261, 327, 1388
Eco31I	GGTCTC	2 cut(s)	1631, 1847
Eco47I	GGWCC	4 cut(s)	283, 424, 979, 1009
Eco52I	CGGCCG	1 cut(s)	1921
Eco53kI	GAGCTC	1 cut(s)	259
Eco57I	CTGAAG	3 cut(s)	899, 965, 1764
Eco88I	CYCGRG	2 cut(s)	102, 1488
EcoICRI	GAGCTC	1 cut(s)	259
EcoNI	CCTNNNNNAGG	1 cut(s)	2153
EcoRII	CCWGG	6 cut(s)	93, 279, 514, 1727, 2008, 2154
EcoT14I	CCWWGG	2 cut(s)	666, 2179
EcoT22I	ATGCAT	1 cut(s)	1469
EcoT38I	GRGCYC	3 cut(s)	261, 327, 1388
ErhI	CCWWGG	2 cut(s)	666, 2179
FaeI	CATG	7 cut(s)	76, 995, 1150, 1316, 1789, 1957, 1991
FalI	AAGNNNNNCTT	2 cut(s)	1014, 1046
FatI	CATG	7 cut(s)	72, 991, 1146, 1312, 1785, 1953, 1987
FauI	CCCGC	1 cut(s)	230
FauNDI	CATATG	3 cut(s)	1228, 1469, 2110
FbaI	TGATCA	1 cut(s)	1498
FblI	GTMKAC	1 cut(s)	1135
FokI	GGATG	5 cut(s)	917, 1175, 2025, 2048, 2185
FriOI	GRGCYC	3 cut(s)	261, 327, 1388
FspAI	RTGCGCAY	1 cut(s)	2040
FspBI	CTAG	2 cut(s)	330, 777
FspI	TGCGCA	1 cut(s)	2040
GlaI	GCGC	4 cut(s)	91, 430, 738, 2040
GsaI	CCCAGC	3 cut(s)	424, 657, 2107
GsuI	CTGGAG	1 cut(s)	1595
HaeII	RGCGCY	1 cut(s)	740
HaeIII	GGCC	5 cut(s)	108, 824, 1923, 2102, 2178
HapII	CCGG	8 cut(s)	56, 132, 138, 859, 960, 1489, 1924, 1933
HgaI	GACGC	7 cut(s)	107, 256, 401, 839, 1016, 1222, 1901
HhaI	GCGC	4 cut(s)	92, 431, 739, 2041
Hin1I	GRCGYC	4 cut(s)	21, 99, 393, 1027
Hin1II	CATG	7 cut(s)	76, 995, 1150, 1316, 1789, 1957, 1991
Hin6I	GCGC	4 cut(s)	90, 429, 737, 2039
HinP1I	GCGC	4 cut(s)	90, 429, 737, 2039
HincII	GTYRAC	2 cut(s)	352, 1075
HindII	GTYRAC	2 cut(s)	352, 1075
HindIII	AAGCTT	2 cut(s)	1438, 1686
HinfI	GANTC	6 cut(s)	376, 383, 683, 784, 1432, 2184
HpaI	GTTAAC	2 cut(s)	352, 1075
HpaII	CCGG	8 cut(s)	56, 132, 138, 859, 960, 1489, 1924, 1933
HphI	GGTGA	3 cut(s)	16, 160, 1477
Hpy166II	GTNNAC	8 cut(s)	31, 232, 352, 391, 523, 1075, 1136, 1809
Hpy188I	TCNGA	6 cut(s)	296, 918, 945, 1863, 2031, 2093
Hpy8I	GTNNAC	8 cut(s)	31, 232, 352, 391, 523, 1075, 1136, 1809
Hpy99I	CGWCG	2 cut(s)	23, 1216
HpyAV	CCTTC	8 cut(s)	491, 673, 774, 940, 1016, 1045, 1739, 1865
HpyCH4III	ACNGT	4 cut(s)	28, 229, 505, 1793
HpyCH4IV	ACGT	3 cut(s)	21, 1044, 1811
HpyCH4V	TGCA	9 cut(s)	219, 532, 676, 799, 991, 1300, 1467, 1615, 1832
HpyF3I	CTNAG	6 cut(s)	740, 788, 1304, 2001, 2030, 2090
HpySE526I	ACGT	3 cut(s)	21, 1044, 1811
Hsp92I	GRCGYC	4 cut(s)	21, 99, 393, 1027
Hsp92II	CATG	7 cut(s)	76, 995, 1150, 1316, 1789, 1957, 1991
HspAI	GCGC	4 cut(s)	90, 429, 737, 2039
Ksp22I	TGATCA	1 cut(s)	1498
KspAI	GTTAAC	2 cut(s)	352, 1075
LmnI	GCTCC	3 cut(s)	58, 270, 1054
Lsp1109I	GCAGC	9 cut(s)	811, 1312, 1554, 1557, 1627, 1894, 1992, 2139, 2142
LweI	GCATC	6 cut(s)	298, 786, 895, 1306, 2026, 2050
MabI	ACCWGGT	1 cut(s)	279
MaeI	CTAG	2 cut(s)	330, 777
MaeII	ACGT	3 cut(s)	21, 1044, 1811
MaeIII	GTNAC	3 cut(s)	22, 623, 1449
MboII	GAAGA	7 cut(s)	256, 565, 849, 1014, 1400, 1705, 2152
MflI	RGATCY	3 cut(s)	309, 1288, 2016
MhlI	GDGCHC	6 cut(s)	261, 327, 572, 960, 1388, 2057
MluCI	AATT	8 cut(s)	407, 807, 1098, 1161, 1251, 1414, 1520, 1555
MlyI	GAGTC	3 cut(s)	377, 692, 1441
Mph1103I	ATGCAT	1 cut(s)	1469
MseI	TTAA	5 cut(s)	351, 1074, 1083, 1419, 1814
MslI	CAYNNNNRTG	1 cut(s)	2060
MspA1I	CMGCKG	2 cut(s)	420, 2133
MspI	CCGG	8 cut(s)	56, 132, 138, 859, 960, 1489, 1924, 1933
Mva1269I	GAATGC	2 cut(s)	1278, 1469
MvaI	CCWGG	6 cut(s)	95, 281, 516, 1729, 2010, 2156
MvnI	CGCG	5 cut(s)	90, 381, 429, 437, 1967
NciI	CCSGG	5 cut(s)	56, 960, 1489, 1490, 1925
NdeI	CATATG	3 cut(s)	1228, 1469, 2110
NlaIII	CATG	7 cut(s)	76, 995, 1150, 1316, 1789, 1957, 1991
NlaIV	GGNNCC	7 cut(s)	136, 425, 446, 502, 569, 1378, 2018
NmeAIII	GCCGAG	3 cut(s)	280, 397, 483
NmuCI	GTSAC	2 cut(s)	22, 1449
NruI	TCGCGA	1 cut(s)	381
NsbI	TGCGCA	1 cut(s)	2040
NsiI	ATGCAT	1 cut(s)	1469
NspI	RCATGY	2 cut(s)	76, 995
PagI	TCATGA	3 cut(s)	1146, 1312, 1987
PcsI	WCGNNNNNNNCGW	1 cut(s)	24
PctI	GAATGC	2 cut(s)	1278, 1469
PfeI	GAWTC	3 cut(s)	376, 784, 2184
PflFI	GACNNNGTC	1 cut(s)	20
PflMI	CCANNNNNTGG	2 cut(s)	238, 1648
Ple19I	CGATCG	1 cut(s)	441
PleI	GAGTC	3 cut(s)	377, 691, 1440
PpsI	GAGTC	3 cut(s)	377, 691, 1440
PshBI	ATTAAT	1 cut(s)	1083
Psp124BI	GAGCTC	1 cut(s)	261
Psp1406I	AACGTT	1 cut(s)	1044
Psp6I	CCWGG	6 cut(s)	93, 279, 514, 1727, 2008, 2154
PspFI	CCCAGC	3 cut(s)	420, 653, 2103
PspGI	CCWGG	6 cut(s)	93, 279, 514, 1727, 2008, 2154
PspN4I	GGNNCC	7 cut(s)	136, 425, 446, 502, 569, 1378, 2018
PspPI	GGNCC	6 cut(s)	283, 424, 979, 1009, 2101, 2176
PstNI	CAGNNNCTG	1 cut(s)	1292
PsuI	RGATCY	3 cut(s)	309, 1288, 2016
PsyI	GACNNNGTC	1 cut(s)	20
PvuI	CGATCG	1 cut(s)	441
PvuII	CAGCTG	1 cut(s)	420
RruI	TCGCGA	1 cut(s)	381
RsaI	GTAC	5 cut(s)	32, 1516, 1790, 1808, 1844
RsaNI	GTAC	5 cut(s)	31, 1515, 1789, 1807, 1843
RseI	CAYNNNNRTG	1 cut(s)	2060
SacI	GAGCTC	1 cut(s)	261
SaqAI	TTAA	5 cut(s)	351, 1074, 1083, 1419, 1814
Sau96I	GGNCC	6 cut(s)	283, 424, 979, 1009, 2101, 2176
ScaI	AGTACT	1 cut(s)	1844
SchI	GAGTC	3 cut(s)	377, 692, 1441
SduI	GDGCHC	6 cut(s)	261, 327, 572, 960, 1388, 2057
SexAI	ACCWGGT	1 cut(s)	279
SfaNI	GCATC	6 cut(s)	298, 786, 895, 1306, 2026, 2050
SfcI	CTRYAG	1 cut(s)	1722
SinI	GGWCC	4 cut(s)	283, 424, 979, 1009
SmaI	CCCGGG	1 cut(s)	1490
SmiMI	CAYNNNNRTG	1 cut(s)	2060
SmlI	CTYRAG	7 cut(s)	260, 767, 1115, 1381, 1838, 1846, 2050
SmoI	CTYRAG	7 cut(s)	260, 767, 1115, 1381, 1838, 1846, 2050
Sse9I	AATT	8 cut(s)	407, 807, 1098, 1161, 1251, 1414, 1520, 1555
SspI	AATATT	1 cut(s)	967
SspMI	CTAG	2 cut(s)	330, 777
SstI	GAGCTC	1 cut(s)	261
StyI	CCWWGG	2 cut(s)	666, 2179
TaaI	ACNGT	4 cut(s)	28, 229, 505, 1793
TaiI	ACGT	3 cut(s)	24, 1047, 1814
TaqI	TCGA	7 cut(s)	120, 699, 732, 1020, 1348, 1435, 1605
TasI	AATT	8 cut(s)	407, 807, 1098, 1161, 1251, 1414, 1520, 1555
TatI	WGTACW	3 cut(s)	30, 1806, 1842
TauI	GCSGC	7 cut(s)	371, 398, 405, 434, 437, 605, 2136
TfiI	GAWTC	3 cut(s)	376, 784, 2184
Tru1I	TTAA	5 cut(s)	351, 1074, 1083, 1419, 1814
Tru9I	TTAA	5 cut(s)	351, 1074, 1083, 1419, 1814
TscAI	CASTG	2 cut(s)	116, 2062
TseFI	GTSAC	2 cut(s)	22, 1449
TseI	GCWGC	9 cut(s)	799, 1300, 1567, 1570, 1615, 1907, 2005, 2127, 2130
Tsp45I	GTSAC	2 cut(s)	22, 1449
TspDTI	ATGAA	3 cut(s)	17, 1621, 2208
TspGWI	ACGGA	2 cut(s)	376, 917
TspMI	CCCGGG	1 cut(s)	1488
TspRI	CASTG	2 cut(s)	116, 2062
Tth111I	GACNNNGTC	1 cut(s)	20
Van91I	CCANNNNNTGG	2 cut(s)	238, 1648
VpaK11BI	GGWCC	4 cut(s)	283, 424, 979, 1009
VspI	ATTAAT	1 cut(s)	1083
XagI	CCTNNNNNAGG	1 cut(s)	2153
XapI	RAATTY	1 cut(s)	1520
XceI	RCATGY	2 cut(s)	76, 995
XmaI	CCCGGG	1 cut(s)	1488
XmiI	GTMKAC	1 cut(s)	1135
XspI	CTAG	2 cut(s)	330, 777
ZraI	GACGTC	1 cut(s)	22
ZrmI	AGTACT	1 cut(s)	1844
Zsp2I	ATGCAT	1 cut(s)	1469

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.