Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0010853

Physical Location & Seq

Genomic Coordinates Forward (+)

19387504 .. 19393693

Gene Structure 6190 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g23690.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGAAGTTCCAGATAGACGACATCACCGTATACTTCCCCTACGACCACATCTACCCAGAACAATACGCCTACATGCTCGAACTCAAACGCACTCTCGACGCGCGCGGCCACTGCCTCCTCGAAATGCCCACCGGCACTGGCAAAACCATCGCCCTCCTCTCCCTCATCACCAGCTACACTCTCTCCAAGCCCCACAACCCCGTCAAGCTCATCTACTGCACCCGCACCGTCCACGAGATGGAGAAGACCCTCGCCGAGCTCAAGCTCCTCCACAACTACCAGCTCCGCCACCTCGGAAACCACGCCGCGATCCTCGCCATCGGCCTCTCCAGCCGGAAGAACCTCTGCGTTAACCCTACCGTCCTCGCCGCCGAGAATCGCGACTCCGTCGACGCCGCGTGCCGGAAACTGACGGCCAGCTGGGTCCGCGCCGCGGCGGTTGCGAACCCTAATGTCCCGAGCTGTGAGTTTTTCGAGCAGTTCGAGAGGGACCGGGAGGGTACGGTGTTGCCTCCTGGGGTTTACACATTGCAGGATTTGAGGGCGTTTGGGAAGGCGAAAAGGTGGTGCCCTTATTTTTTGGCGAGGAGAATGGTGGAGGCGGCCAATGTGGTGGTTTATAGCTATCAGTATTTGCTGGATCCCAAGGTGGCGGGGATTATATCCAAGGAGATGCAGAAGGAGTCGGTTGTGGTGTTTGATGAGGCTCATAATATCGACAATGTGTGTATTGAGGCACTTAGTGTCAGTGTGAGGAGGCAGACGCTTGAGGGGGCCAGGAGGAATCTCAGTAAGATGCAGCAGGAAATTGAAAGGTTCAAGGCCACAGACGCAGGAAGATTGCGACAAGAATACAACCGGCTAGTTGAGGGTTTGGCACAAGGAGGAAACCTACCCATGACGGATGCTTGGCTTCAGAATCCTGCCCTACCTGACGATATTCTGAAGGAGGCTGTGCCCGGGAATATTCGCAAAGCAGATCATTTTCTGCATGTCTTACGAAGATTGGTCCAGTATCTTGAAGACCGTCTTGGAAATGAGAATGTTGAGAAGGAGCTCCCTCTTGTCTTTGTTAAGTCTATTAATACCCAGGCTGGAATTGACCAGAAAACTCTCAAGTTCTGCTATGATCGTCTGCACTCACTCATGATGACATTAGAAATTACTGACACGGATGAATTCTTACACATCCAAACTATATGCGACTTTGCGACGCTTGTGGGAACATATGCTCGTGGTTTCTCTATCATATTTGAGCCATTTGACGAGAGAATGCCACACATTCCTGATCCTGTTTTGCAGCTTAGTTGTCATGATGCTTCTCTTGCCATAAAGCCTGTATTTGATCGATTTCAATCAGTTGTGATTACATCTGGAACCCTGAGCCCAATTGATCTCTATCCCCGTCTTCTCAATTTTAACCCTGTTGTGAGTCGAAGCTTTACAATGTCACTAACCAGAGAATGCATATGCCCAATGGTTCTCACCCGTGGAAGTGATCAGCTTCCTGTTAGTACAAAATTTGATATGAGAAGTGATCTCGGAGTGGTAAGGAATTATGGGAGGCTTCTGCTGGAGATGGTTTCTGTTGTTCCAGATGGGGTTGTATGTTTTTTCGTCAGCTACTCATATATGGATGGTATAGTGAACAGCTGGAATGAAAGTGGAATATTAAAGGAGATAATGCAGCATAAGCTCGTGTTTATTGAGACCCAGGACGTGGTAGAGACGACTTTGGCTTTGGACAACTACCGCAAAGCTTGCGATTGTGGGAGAGGTGCTGTCTTCTTTTCTGTAGCGAGGGGAAAGGTAGCTGAAGGAATAGACTTTGATCGTCATTATGGACGATTGGTCATCATGTACGGTGTTCCTTTTCAGTACACGTTAAGCAAGATTTTGGTTGCGCGGCTCGAGTACTTGAGGGAGAACTTTCAGATAAAGGAAGGAGATTTCTTGACCTTCGATGCTTTGAGACAAGCTGCCCAATGTGTCGGCAGGGTCATCCGATCAAAAGCGGACTACGGAATGATGATTTTCGCGGATAAAAGATATAGCCGACATGACAAGCGGTCTAAGTTGCCAGGATGGATCCTCTCTCATCTGAGGGATGCACACCTCAACTTGAGCACAGATATGGCTCTCCATATAGCACGAGAGTTCCTCAGAAAGATGGCCCAGCCATATGACAAGACTGGCAGCAGCAGCGGCAGGAAGACACTCCTGTCCCAGGATGACTTGGAGAGGATGGGCCAAGGAATCACTGATGAAATGTTGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

DEAD_2 HBB Helicase_C_2

Protein Analysis

760

Amino Acids

86.45

Weight (kDa)

7.55

Isoelectric Point (pI)

44.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	72 - 256	1.1e-51	DEAD_2
HBB	PF06777	270 - 412	8.2e-40	Helical and beta-bridge domain
Helicase_C_2	PF13307	524 - 697	3.4e-38	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03190 AT1G03190
fragaria_vesca	FvH4_2g23690
malus_domestica	MD03G1266200.v1.1 MD11G1286800.v1.1
prunus_persica	Prupe.8G242000_v2.0.a1 Prupe.8G242000_v2.0.a1
pyrus_communis	pycom03g21270
rosa_chinensis	RchiOBHm_Chr6g0291151
rosa_laevigata	RLG00000012140
rosa_multiflora	Rmu_co8311277.1_g000001 Rmu_sc0004642.1_g000023 Rmu_sc0013275.1_g000002
rosa_roxburghii	Rroxscaffold_7G00176340
rosa_rugosa	Rorug06G0218000
rosa_samantha	Rh6AG328400 Rh6BG336000 Rh6CG342000 Rh6DG329300
rosa_wichuraiana	Rw6G028570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2221
AccB1I	GGYRCC	1 cut(s)	567
AccB7I	CCANNNNNTGG	2 cut(s)	238, 1720
AccI	GTMKAC	2 cut(s)	30, 390
AclWI	GGATC	6 cut(s)	304, 635, 648, 1283, 2083, 2096
AcoI	YGGCCR	3 cut(s)	106, 414, 603
AcsI	RAATTY	2 cut(s)	1178, 1520
AcuI	CTGAAG	3 cut(s)	899, 965, 1836
AcyI	GRCGYC	1 cut(s)	393
AdeI	CACNNNGTG	1 cut(s)	743
AfaI	GTAC	5 cut(s)	502, 1516, 1862, 1880, 1916
AfiI	CCNNNNNNNGG	5 cut(s)	238, 402, 433, 1720, 2227
AflIII	ACRYGT	1 cut(s)	1881
AgsI	TTSAA	4 cut(s)	812, 820, 1022, 1355
AhdI	GACNNNNNGTC	2 cut(s)	1850, 2068
AjiI	CACGTC	1 cut(s)	1720
AjnI	CCWGG	6 cut(s)	514, 776, 1089, 1713, 2080, 2226
AjuI	GAANNNNNNNTTGG	2 cut(s)	1014, 1046
Alw21I	GWGCWC	3 cut(s)	261, 1059, 2129
Alw26I	GTCTC	3 cut(s)	1703, 1721, 1966
AlwI	GGATC	6 cut(s)	304, 635, 648, 1283, 2083, 2096
Ama87I	CYCGRG	3 cut(s)	457, 959, 1910
AoxI	GGCC	8 cut(s)	106, 322, 414, 603, 774, 822, 2172, 2248
ApeKI	GCWGC	7 cut(s)	799, 1300, 1687, 1979, 2196, 2199, 2202
ApoI	RAATTY	2 cut(s)	1178, 1520
ArsI	GACNNNNNNTTYG	2 cut(s)	1723, 1755
AseI	ATTAAT	1 cut(s)	1083
AspLEI	GCGC	4 cut(s)	103, 105, 431, 1906
AspS9I	GGNCC	6 cut(s)	424, 490, 774, 1009, 2173, 2248
AsuC2I	CCSGG	3 cut(s)	494, 960, 961
AsuHPI	GGTGA	3 cut(s)	16, 160, 1477
AvaI	CYCGRG	3 cut(s)	457, 959, 1910
AvaII	GGWCC	3 cut(s)	424, 490, 1009
BaeGI	GKGCMC	2 cut(s)	572, 960
BamHI	GGATCC	2 cut(s)	640, 2088
BanI	GGYRCC	1 cut(s)	567
BanII	GRGCYC	3 cut(s)	261, 1059, 1388
BauI	CACGAG	4 cut(s)	233, 1233, 1697, 2151
BbsI	GAAGAC	5 cut(s)	251, 1029, 1400, 1777, 2219
Bbv12I	GWGCWC	3 cut(s)	261, 1059, 2129
BbvI	GCAGC	7 cut(s)	811, 1312, 1699, 1966, 2208, 2211, 2214
BccI	CCATC	9 cut(s)	155, 232, 326, 1573, 1592, 1631, 2079, 2164, 2239
BceAI	ACGGC	1 cut(s)	429
BcgI	CGANNNNNNTGC	2 cut(s)	130, 164
BciT130I	CCWGG	6 cut(s)	516, 778, 1091, 1715, 2082, 2228
BclI	TGATCA	1 cut(s)	1498
BcnI	CCSGG	3 cut(s)	494, 960, 961
BcoDI	GTCTC	3 cut(s)	1703, 1721, 1966
BfaI	CTAG	2 cut(s)	863, 2278
BfmI	CTRYAG	1 cut(s)	1794
BmcAI	AGTACT	1 cut(s)	1916
Bme1390I	CCNGG	9 cut(s)	494, 516, 778, 960, 961, 1091, 1715, 2082, 2228
Bme18I	GGWCC	3 cut(s)	424, 490, 1009
BmeRI	GACNNNNNGTC	2 cut(s)	1850, 2068
BmeT110I	CYCGRG	3 cut(s)	457, 959, 1910
BmgBI	CACGTC	1 cut(s)	1720
BmgT120I	GGNCC	6 cut(s)	424, 490, 774, 1009, 2173, 2248
BmiI	GGNNCC	7 cut(s)	425, 491, 569, 642, 775, 1378, 2090
BmrFI	CCNGG	9 cut(s)	494, 516, 778, 960, 961, 1091, 1715, 2082, 2228
BmsI	GCATC	6 cut(s)	663, 786, 895, 1306, 1954, 2098
BpiI	GAAGAC	5 cut(s)	251, 1029, 1400, 1777, 2219
BplI	GAGNNNNNCTC	2 cut(s)	2145, 2177
BpmI	CTGGAG	2 cut(s)	313, 1595
Bpu10I	CCTNAGC	1 cut(s)	1382
BpuEI	CTTGAG	5 cut(s)	245, 788, 1100, 1939, 2143
BpuMI	CCSGG	3 cut(s)	494, 960, 961
Bsa29I	ATCGAT	1 cut(s)	1348
BsaBI	GATNNNNATC	2 cut(s)	1354, 1398
BsaHI	GRCGYC	1 cut(s)	393
BsaI	GGTCTC	1 cut(s)	1703
BsaXI	ACNNNNNCTCC	2 cut(s)	167, 197
Bsc4I	CCNNNNNNNGG	5 cut(s)	238, 402, 433, 1720, 2227
Bse118I	RCCGGY	2 cut(s)	131, 858
Bse1I	ACTGG	3 cut(s)	142, 1012, 2197
Bse3DI	GCAATG	1 cut(s)	527
Bse8I	GATNNNNATC	2 cut(s)	1354, 1398
BseBI	CCWGG	6 cut(s)	516, 778, 1091, 1715, 2082, 2228
BseCI	ATCGAT	1 cut(s)	1348
BseGI	GGATG	9 cut(s)	910, 1180, 1188, 1642, 2001, 2090, 2113, 2236, 2250
BseJI	GATNNNNATC	2 cut(s)	1354, 1398
BseLI	CCNNNNNNNGG	5 cut(s)	238, 402, 433, 1720, 2227
BseMI	GCAATG	1 cut(s)	527
BseMII	CTCAG	4 cut(s)	802, 1373, 2093, 2176
BseNI	ACTGG	3 cut(s)	142, 1012, 2197
BsePI	GCGCGC	1 cut(s)	101
BseRI	GAGGAG	5 cut(s)	107, 146, 257, 601, 769
BseSI	GKGCMC	2 cut(s)	572, 960
BseXI	GCAGC	7 cut(s)	811, 1312, 1699, 1966, 2208, 2211, 2214
BseYI	CCCAGC	2 cut(s)	420, 2175
BsgI	GTGCAG	2 cut(s)	202, 1121
BshFI	GGCC	8 cut(s)	108, 324, 416, 605, 776, 824, 2174, 2250
BshNI	GGYRCC	1 cut(s)	567
BshVI	ATCGAT	1 cut(s)	1348
BsiHKAI	GWGCWC	3 cut(s)	261, 1059, 2129
BsiHKCI	CYCGRG	3 cut(s)	457, 959, 1910
BsiSI	CCGG	6 cut(s)	132, 334, 403, 493, 859, 960
BslFI	GGGAC	3 cut(s)	440, 503, 2209
BslI	CCNNNNNNNGG	5 cut(s)	238, 402, 433, 1720, 2227
BsmAI	GTCTC	3 cut(s)	1703, 1721, 1966
BsmBI	CGTCTC	1 cut(s)	1721
BsmFI	GGGAC	3 cut(s)	440, 503, 2209
BsmI	GAATGC	2 cut(s)	1278, 1469
BsnI	GGCC	8 cut(s)	108, 324, 416, 605, 776, 824, 2174, 2250
Bso31I	GGTCTC	1 cut(s)	1703
BsoBI	CYCGRG	3 cut(s)	457, 959, 1910
Bsp1286I	GDGCHC	6 cut(s)	261, 572, 960, 1059, 1388, 2129
Bsp68I	TCGCGA	1 cut(s)	381
BspANI	GGCC	8 cut(s)	108, 324, 416, 605, 776, 824, 2174, 2250
BspCNI	CTCAG	4 cut(s)	801, 1374, 2094, 2175
BspDI	ATCGAT	1 cut(s)	1348
BspHI	TCATGA	2 cut(s)	1146, 1312
BspLI	GGNNCC	7 cut(s)	425, 491, 569, 642, 775, 1378, 2090
BspPI	GGATC	6 cut(s)	304, 635, 648, 1283, 2083, 2096
BspT107I	GGYRCC	1 cut(s)	567
BspTNI	GGTCTC	1 cut(s)	1703
BsrDI	GCAATG	1 cut(s)	527
BsrFI	RCCGGY	2 cut(s)	131, 858
BsrI	ACTGG	3 cut(s)	142, 1012, 2197
BssAI	RCCGGY	2 cut(s)	131, 858
BssHII	GCGCGC	1 cut(s)	101
BssNAI	GTATAC	1 cut(s)	31
BssNI	GRCGYC	1 cut(s)	393
BssSI	CACGAG	4 cut(s)	233, 1233, 1697, 2151
BssT1I	CCWWGG	3 cut(s)	645, 666, 2251
Bst1107I	GTATAC	1 cut(s)	31
Bst2BI	CACGAG	4 cut(s)	233, 1233, 1697, 2151
Bst2UI	CCWGG	6 cut(s)	516, 778, 1091, 1715, 2082, 2228
Bst4CI	ACNGT	6 cut(s)	28, 229, 361, 505, 1028, 1865
BstACI	GRCGYC	1 cut(s)	393
BstAPI	GCANNNNNTGC	1 cut(s)	1761
BstC8I	GCNNGC	4 cut(s)	103, 400, 418, 1762
BstDEI	CTNAG	7 cut(s)	740, 788, 1304, 1382, 2073, 2102, 2162
BstDSI	CCRYGG	2 cut(s)	432, 1489
BstENI	CCTNNNNNAGG	1 cut(s)	2225
BstF5I	GGATG	9 cut(s)	910, 1180, 1188, 1642, 2001, 2090, 2113, 2236, 2250
BstHHI	GCGC	4 cut(s)	103, 105, 431, 1906
BstMAI	GTCTC	3 cut(s)	1703, 1721, 1966
BstNI	CCWGG	6 cut(s)	516, 778, 1091, 1715, 2082, 2228
BstNSI	RCATGY	2 cut(s)	76, 995
BstSCI	CCNGG	9 cut(s)	492, 514, 776, 958, 959, 1089, 1713, 2080, 2226
BstSFI	CTRYAG	1 cut(s)	1794
BstSLI	GKGCMC	2 cut(s)	572, 960
BstV1I	GCAGC	7 cut(s)	811, 1312, 1699, 1966, 2208, 2211, 2214
BstV2I	GAAGAC	5 cut(s)	251, 1029, 1400, 1777, 2219
BstX2I	RGATCY	2 cut(s)	640, 2088
BstXI	CCANNNNNNTGG	1 cut(s)	613
BstYI	RGATCY	2 cut(s)	640, 2088
BstZ17I	GTATAC	1 cut(s)	31
Bsu15I	ATCGAT	1 cut(s)	1348
BsuRI	GGCC	8 cut(s)	108, 324, 416, 605, 776, 824, 2174, 2250
BsuTUI	ATCGAT	1 cut(s)	1348
BtgI	CCRYGG	2 cut(s)	432, 1489
BtgZI	GCGATG	1 cut(s)	133
BtrI	CACGTC	1 cut(s)	1720
BtsCI	GGATG	9 cut(s)	910, 1180, 1188, 1642, 2001, 2090, 2113, 2236, 2250
BtsI	GCAGTG	1 cut(s)	109
BtsIMutI	CAGTG	4 cut(s)	109, 135, 754, 2259
BtuMI	TCGCGA	1 cut(s)	381
Cac8I	GCNNGC	4 cut(s)	103, 400, 418, 1762
CciI	TCATGA	2 cut(s)	1146, 1312
CfoI	GCGC	4 cut(s)	103, 105, 431, 1906
Cfr10I	RCCGGY	2 cut(s)	131, 858
Cfr13I	GGNCC	6 cut(s)	424, 490, 774, 1009, 2173, 2248
Cfr42I	CCGCGG	1 cut(s)	435
Cfr9I	CCCGGG	1 cut(s)	959
ClaI	ATCGAT	1 cut(s)	1348
CseI	GACGC	5 cut(s)	107, 401, 772, 839, 1222
Csp6I	GTAC	5 cut(s)	501, 1515, 1861, 1879, 1915
CviAII	CATG	7 cut(s)	73, 898, 992, 1147, 1313, 1858, 2060
CviQI	GTAC	5 cut(s)	501, 1515, 1861, 1879, 1915
DdeI	CTNAG	7 cut(s)	740, 788, 1304, 1382, 2073, 2102, 2162
DraIII	CACNNNGTG	1 cut(s)	743
DrdI	GACNNNNNNGTC	1 cut(s)	2221
DriI	GACNNNNNGTC	2 cut(s)	1850, 2068
DseDI	GACNNNNNNGTC	1 cut(s)	2221
EaeI	YGGCCR	3 cut(s)	106, 414, 603
Eam1105I	GACNNNNNGTC	2 cut(s)	1850, 2068
EciI	GGCGGA	1 cut(s)	275
Ecl136II	GAGCTC	2 cut(s)	259, 1057
Eco130I	CCWWGG	3 cut(s)	645, 666, 2251
Eco24I	GRGCYC	3 cut(s)	261, 1059, 1388
Eco31I	GGTCTC	1 cut(s)	1703
Eco47I	GGWCC	3 cut(s)	424, 490, 1009
Eco53kI	GAGCTC	2 cut(s)	259, 1057
Eco57I	CTGAAG	3 cut(s)	899, 965, 1836
Eco88I	CYCGRG	3 cut(s)	457, 959, 1910
EcoICRI	GAGCTC	2 cut(s)	259, 1057
EcoNI	CCTNNNNNAGG	1 cut(s)	2225
EcoRI	GAATTC	1 cut(s)	1178
EcoRII	CCWGG	6 cut(s)	514, 776, 1089, 1713, 2080, 2226
EcoT14I	CCWWGG	3 cut(s)	645, 666, 2251
EcoT22I	ATGCAT	1 cut(s)	1469
EcoT38I	GRGCYC	3 cut(s)	261, 1059, 1388
ErhI	CCWWGG	3 cut(s)	645, 666, 2251
Esp3I	CGTCTC	1 cut(s)	1721
FaeI	CATG	7 cut(s)	76, 901, 995, 1150, 1316, 1861, 2063
FalI	AAGNNNNNCTT	2 cut(s)	1014, 1046
FaqI	GGGAC	3 cut(s)	440, 503, 2209
FatI	CATG	7 cut(s)	72, 897, 991, 1146, 1312, 1857, 2059
FauI	CCCGC	2 cut(s)	230, 646
FauNDI	CATATG	3 cut(s)	1228, 1469, 2182
FbaI	TGATCA	1 cut(s)	1498
FblI	GTMKAC	2 cut(s)	30, 390
FokI	GGATG	9 cut(s)	917, 1175, 1187, 1649, 1988, 2097, 2120, 2243, 2257
FriOI	GRGCYC	3 cut(s)	261, 1059, 1388
FspBI	CTAG	2 cut(s)	863, 2278
GlaI	GCGC	4 cut(s)	102, 104, 430, 1905
GsaI	CCCAGC	2 cut(s)	424, 2179
GsuI	CTGGAG	2 cut(s)	313, 1595
HaeIII	GGCC	8 cut(s)	108, 324, 416, 605, 776, 824, 2174, 2250
HapII	CCGG	6 cut(s)	132, 334, 403, 493, 859, 960
HgaI	GACGC	5 cut(s)	107, 401, 772, 839, 1222
HhaI	GCGC	4 cut(s)	103, 105, 431, 1906
Hin1I	GRCGYC	1 cut(s)	393
Hin1II	CATG	7 cut(s)	76, 901, 995, 1150, 1316, 1861, 2063
Hin6I	GCGC	4 cut(s)	101, 103, 429, 1904
HinP1I	GCGC	4 cut(s)	101, 103, 429, 1904
HincII	GTYRAC	2 cut(s)	352, 391
HindII	GTYRAC	2 cut(s)	352, 391
HindIII	AAGCTT	2 cut(s)	1438, 1758
HinfI	GANTC	7 cut(s)	376, 383, 683, 784, 919, 1432, 2256
HpaI	GTTAAC	1 cut(s)	352
HpaII	CCGG	6 cut(s)	132, 334, 403, 493, 859, 960
HphI	GGTGA	3 cut(s)	16, 160, 1477
Hpy166II	GTNNAC	7 cut(s)	31, 232, 352, 391, 523, 1648, 1881
Hpy188I	TCNGA	8 cut(s)	296, 918, 945, 1544, 1935, 2006, 2103, 2165
Hpy8I	GTNNAC	7 cut(s)	31, 232, 352, 391, 523, 1648, 1881
Hpy99I	CGWCG	4 cut(s)	101, 392, 395, 1216
HpyAV	CCTTC	7 cut(s)	547, 673, 940, 1045, 1811, 1937, 1969
HpyCH4III	ACNGT	6 cut(s)	28, 229, 361, 505, 1028, 1865
HpyCH4IV	ACGT	2 cut(s)	1719, 1883
HpyF3I	CTNAG	7 cut(s)	740, 788, 1304, 1382, 2073, 2102, 2162
HpySE526I	ACGT	2 cut(s)	1719, 1883
Hsp92I	GRCGYC	1 cut(s)	393
Hsp92II	CATG	7 cut(s)	76, 901, 995, 1150, 1316, 1861, 2063
HspAI	GCGC	4 cut(s)	101, 103, 429, 1904
Ksp22I	TGATCA	1 cut(s)	1498
KspAI	GTTAAC	1 cut(s)	352
KspI	CCGCGG	1 cut(s)	435
LmnI	GCTCC	4 cut(s)	270, 288, 1054, 1062
Lsp1109I	GCAGC	7 cut(s)	811, 1312, 1699, 1966, 2208, 2211, 2214
LweI	GCATC	6 cut(s)	663, 786, 895, 1306, 1954, 2098
MaeI	CTAG	2 cut(s)	863, 2278
MaeII	ACGT	2 cut(s)	1719, 1883
MaeIII	GTNAC	1 cut(s)	1449
MauBI	CGCGCGCG	1 cut(s)	101
MboII	GAAGA	8 cut(s)	256, 349, 849, 1014, 1034, 1400, 1777, 2224
MfeI	CAATTG	1 cut(s)	1389
MflI	RGATCY	2 cut(s)	640, 2088
MhlI	GDGCHC	6 cut(s)	261, 572, 960, 1059, 1388, 2129
MluCI	AATT	8 cut(s)	807, 1098, 1161, 1178, 1389, 1414, 1520, 1555
MlyI	GAGTC	3 cut(s)	377, 692, 1441
Mph1103I	ATGCAT	1 cut(s)	1469
MseI	TTAA	6 cut(s)	351, 1074, 1083, 1419, 1673, 1886
MslI	CAYNNNNRTG	1 cut(s)	2132
MspA1I	CMGCKG	4 cut(s)	420, 434, 1653, 2205
MspI	CCGG	6 cut(s)	132, 334, 403, 493, 859, 960
MspR9I	CCNGG	9 cut(s)	494, 516, 778, 960, 961, 1091, 1715, 2082, 2228
MunI	CAATTG	1 cut(s)	1389
Mva1269I	GAATGC	2 cut(s)	1278, 1469
MvaI	CCWGG	6 cut(s)	516, 778, 1091, 1715, 2082, 2228
NciI	CCSGG	3 cut(s)	494, 960, 961
NdeI	CATATG	3 cut(s)	1228, 1469, 2182
NlaIII	CATG	7 cut(s)	76, 901, 995, 1150, 1316, 1861, 2063
NlaIV	GGNNCC	7 cut(s)	425, 491, 569, 642, 775, 1378, 2090
NmeAIII	GCCGAG	2 cut(s)	280, 397
NmuCI	GTSAC	1 cut(s)	1449
NruI	TCGCGA	1 cut(s)	381
NsiI	ATGCAT	1 cut(s)	1469
NspI	RCATGY	2 cut(s)	76, 995
PaeR7I	CTCGAG	1 cut(s)	1910
PagI	TCATGA	2 cut(s)	1146, 1312
PauI	GCGCGC	1 cut(s)	101
PcsI	WCGNNNNNNNCGW	2 cut(s)	24, 480
PctI	GAATGC	2 cut(s)	1278, 1469
PfeI	GAWTC	4 cut(s)	376, 784, 919, 2256
PflFI	GACNNNGTC	1 cut(s)	386
PflMI	CCANNNNNTGG	2 cut(s)	238, 1720
PleI	GAGTC	3 cut(s)	377, 691, 1440
PpsI	GAGTC	3 cut(s)	377, 691, 1440
PshBI	ATTAAT	1 cut(s)	1083
Psp124BI	GAGCTC	2 cut(s)	261, 1059
Psp6I	CCWGG	6 cut(s)	514, 776, 1089, 1713, 2080, 2226
PspFI	CCCAGC	2 cut(s)	420, 2175
PspGI	CCWGG	6 cut(s)	514, 776, 1089, 1713, 2080, 2226
PspN4I	GGNNCC	7 cut(s)	425, 491, 569, 642, 775, 1378, 2090
PspPI	GGNCC	6 cut(s)	424, 490, 774, 1009, 2173, 2248
PspXI	VCTCGAGB	1 cut(s)	1910
PsuI	RGATCY	2 cut(s)	640, 2088
PsyI	GACNNNGTC	1 cut(s)	386
PteI	GCGCGC	1 cut(s)	101
PvuII	CAGCTG	2 cut(s)	420, 1653
RruI	TCGCGA	1 cut(s)	381
RsaI	GTAC	5 cut(s)	502, 1516, 1862, 1880, 1916
RsaNI	GTAC	5 cut(s)	501, 1515, 1861, 1879, 1915
RseI	CAYNNNNRTG	1 cut(s)	2132
SacI	GAGCTC	2 cut(s)	261, 1059
SacII	CCGCGG	1 cut(s)	435
SalI	GTCGAC	1 cut(s)	389
SaqAI	TTAA	6 cut(s)	351, 1074, 1083, 1419, 1673, 1886
Sau96I	GGNCC	6 cut(s)	424, 490, 774, 1009, 2173, 2248
ScaI	AGTACT	1 cut(s)	1916
SchI	GAGTC	3 cut(s)	377, 692, 1441
ScrFI	CCNGG	9 cut(s)	494, 516, 778, 960, 961, 1091, 1715, 2082, 2228
SduI	GDGCHC	6 cut(s)	261, 572, 960, 1059, 1388, 2129
SfaNI	GCATC	6 cut(s)	663, 786, 895, 1306, 1954, 2098
SfcI	CTRYAG	1 cut(s)	1794
Sfr274I	CTCGAG	1 cut(s)	1910
Sfr303I	CCGCGG	1 cut(s)	435
SgrBI	CCGCGG	1 cut(s)	435
SgrDI	CGTCGACG	1 cut(s)	389
SinI	GGWCC	3 cut(s)	424, 490, 1009
SlaI	CTCGAG	1 cut(s)	1910
SmaI	CCCGGG	1 cut(s)	961
SmiMI	CAYNNNNRTG	1 cut(s)	2132
SmlI	CTYRAG	6 cut(s)	260, 767, 1115, 1910, 1918, 2122
SmoI	CTYRAG	6 cut(s)	260, 767, 1115, 1910, 1918, 2122
Sse9I	AATT	8 cut(s)	807, 1098, 1161, 1178, 1389, 1414, 1520, 1555
SspI	AATATT	2 cut(s)	967, 1671
SspMI	CTAG	2 cut(s)	863, 2278
SstI	GAGCTC	2 cut(s)	261, 1059
StyD4I	CCNGG	9 cut(s)	492, 514, 776, 958, 959, 1089, 1713, 2080, 2226
StyI	CCWWGG	3 cut(s)	645, 666, 2251
TaaI	ACNGT	6 cut(s)	28, 229, 361, 505, 1028, 1865
TaiI	ACGT	2 cut(s)	1722, 1886
TasI	AATT	8 cut(s)	807, 1098, 1161, 1178, 1389, 1414, 1520, 1555
TatI	WGTACW	3 cut(s)	1514, 1878, 1914
TauI	GCSGC	9 cut(s)	108, 308, 371, 398, 434, 437, 605, 1909, 2208
TfiI	GAWTC	4 cut(s)	376, 784, 919, 2256
Tru1I	TTAA	6 cut(s)	351, 1074, 1083, 1419, 1673, 1886
Tru9I	TTAA	6 cut(s)	351, 1074, 1083, 1419, 1673, 1886
TscAI	CASTG	4 cut(s)	116, 142, 754, 2266
TseFI	GTSAC	1 cut(s)	1449
TseI	GCWGC	7 cut(s)	799, 1300, 1687, 1979, 2196, 2199, 2202
Tsp45I	GTSAC	1 cut(s)	1449
TspDTI	ATGAA	4 cut(s)	17, 1191, 1674, 2280
TspGWI	ACGGA	4 cut(s)	376, 917, 1187, 2037
TspMI	CCCGGG	1 cut(s)	959
TspRI	CASTG	4 cut(s)	116, 142, 754, 2266
Tth111I	GACNNNGTC	1 cut(s)	386
Van91I	CCANNNNNTGG	2 cut(s)	238, 1720
VpaK11BI	GGWCC	3 cut(s)	424, 490, 1009
VspI	ATTAAT	1 cut(s)	1083
XagI	CCTNNNNNAGG	1 cut(s)	2225
XapI	RAATTY	2 cut(s)	1178, 1520
XceI	RCATGY	2 cut(s)	76, 995
XhoI	CTCGAG	1 cut(s)	1910
XmaI	CCCGGG	1 cut(s)	959
XmiI	GTMKAC	2 cut(s)	30, 390
XspI	CTAG	2 cut(s)	863, 2278
ZrmI	AGTACT	1 cut(s)	1916
Zsp2I	ATGCAT	1 cut(s)	1469

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.