Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0010853

Physical Location & Seq

Genomic Coordinates Forward (+)

40637841 .. 40641074

Gene Structure 3234 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1286800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 969 bp

ATGCCACACATTCCTGATCCTGTTTTACAGCTTTGCTGTCATGATGCTTCACTTGCCATAAAGCCTGTATTTGATCGATTTCAATCAGTTGTGATTACATCTGGAACCTTGAGCCCAATTGATCTCTACCCCCATCTTCTTAATTTCCATCCTGTCATCAGTCGAAGTTTTACTATGTCATTAACAAGAGATTGCATATGCCCAATGGTTCTCACCCGTGGAAGTGATCAGGTTCCAGTAAGTACCAAATATGATATGAGAAGTGATCTTGGCGTGGTAAGGAACTATGGGAGGTTGCTGCTGGAGATGGTTTCTGCTGTTCCAGATGGGGTTGTATGTTTTTTCGTCAGTTACTCTTATATGGATATGATAGTCAATAGCTGGAATGAAAATGGAATTTTGAAGGAAATAATGCAGCATAAGCTTGTTTTCATCGAGACCCAAGATGTGGTAGAGACTACTTTGGCTCCTGACAATTACCGTAAGGCTTGTGACTGTGGGAGGGGTGCTGTCTTCTTGTCTGTTGCCAGAGGAAAAGTAGCTGAAGGAATAGACTTTGATCGATATTATGGACGATTGGTGATCATGTTTGGCATTCCTTTCCAGTACACATTAAGCAAGATTTTGCTTGCACAGCTGGAATACTTGCGGGATACCTTTCAAATAAAGGAGGGAGACTTTTTGACATTTGATGCCTTGAGACAAGCAGCTCAATGTGTAGGCCGAGTCATCCGGTCAAAAGCGGACTATGGCATGAAGATTTTTGCTGACAAAAGATGTAGCCGTCATGACAAGCGATCCAAATTGCCTGGATGGATACTGTCGCATTTACGTGATGCACATCTGAACTTGAGCACGGACATGGCTCTGCATATAGCACGAGAGTTCCTTAGGAAGATGGCTCAACCATATGACAAGGTTGGTGGCGGCAGCGGTAAGAAAACCCTTTTATCCCAAGAAGACATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

DEAD_2 HBB Helicase_C_2

Protein Analysis

323

Amino Acids

36.45

Weight (kDa)

8.5

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C_2	PF13307	100 - 273	2.8e-36	Helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03190 AT1G03190
fragaria_vesca	FvH4_2g23690
malus_domestica	MD03G1266200.v1.1 MD11G1286800.v1.1
prunus_persica	Prupe.8G242000_v2.0.a1 Prupe.8G242000_v2.0.a1
pyrus_communis	pycom03g21270
rosa_chinensis	RchiOBHm_Chr6g0291151
rosa_laevigata	RLG00000012140
rosa_multiflora	Rmu_co8311277.1_g000001 Rmu_sc0004642.1_g000023 Rmu_sc0013275.1_g000002
rosa_roxburghii	Rroxscaffold_7G00176340
rosa_rugosa	Rorug06G0218000
rosa_samantha	Rh6AG328400 Rh6BG336000 Rh6CG342000 Rh6DG329300
rosa_wichuraiana	Rw6G028570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	448
AciI	CCGC	4 cut(s)	649, 743, 927, 933
AclWI	GGATC	2 cut(s)	11, 792
AcsI	RAATTY	1 cut(s)	396
AcuI	CTGAAG	1 cut(s)	564
AfaI	GTAC	2 cut(s)	244, 608
AfiI	CCNNNNNNNGG	1 cut(s)	448
AgsI	TTSAA	3 cut(s)	83, 403, 662
AjnI	CCWGG	1 cut(s)	808
AluBI	AGCT	6 cut(s)	31, 381, 424, 542, 637, 710
AluI	AGCT	6 cut(s)	31, 381, 424, 542, 637, 710
Alw21I	GWGCWC	1 cut(s)	857
Alw26I	GTCTC	4 cut(s)	431, 449, 669, 694
AlwI	GGATC	2 cut(s)	11, 792
AoxI	GGCC	1 cut(s)	721
ApeKI	GCWGC	4 cut(s)	298, 415, 707, 930
ApoI	RAATTY	1 cut(s)	396
AsuHPI	GGTGA	2 cut(s)	205, 592
AxyI	CCTNAGG	1 cut(s)	890
BanII	GRGCYC	1 cut(s)	116
BauI	CACGAG	1 cut(s)	879
BbsI	GAAGAC	1 cut(s)	505
Bbv12I	GWGCWC	1 cut(s)	857
BbvI	GCAGC	4 cut(s)	285, 427, 719, 942
BccI	CCATC	6 cut(s)	141, 156, 301, 320, 807, 892
BceAI	ACGGC	1 cut(s)	768
BciT130I	CCWGG	1 cut(s)	810
BciVI	GTATCC	2 cut(s)	646, 810
BclI	TGATCA	2 cut(s)	226, 582
BcoDI	GTCTC	4 cut(s)	431, 449, 669, 694
BfuI	GTATCC	2 cut(s)	646, 810
BisI	GCNGC	5 cut(s)	299, 416, 708, 928, 931
BlsI	GCNGC	5 cut(s)	300, 417, 709, 929, 932
Bme1390I	CCNGG	1 cut(s)	810
BmiI	GGNNCC	3 cut(s)	106, 234, 468
BmrFI	CCNGG	1 cut(s)	810
BmsI	GCATC	3 cut(s)	34, 682, 826
BpiI	GAAGAC	1 cut(s)	505
BpmI	CTGGAG	1 cut(s)	323
BpuEI	CTTGAG	3 cut(s)	130, 718, 871
Bsa29I	ATCGAT	2 cut(s)	76, 562
BsaAI	YACGTR	1 cut(s)	833
BsaBI	GATNNNNATC	2 cut(s)	82, 840
BsaI	GGTCTC	1 cut(s)	431
BsaJI	CCNNGG	1 cut(s)	217
BsaWI	WCCGGW	1 cut(s)	732
BsaXI	ACNNNNNCTCC	2 cut(s)	451, 481
Bsc4I	CCNNNNNNNGG	1 cut(s)	448
Bse1I	ACTGG	2 cut(s)	236, 604
Bse21I	CCTNAGG	1 cut(s)	890
Bse8I	GATNNNNATC	2 cut(s)	82, 840
BseBI	CCWGG	1 cut(s)	810
BseCI	ATCGAT	2 cut(s)	76, 562
BseDI	CCNNGG	1 cut(s)	217
BseGI	GGATG	3 cut(s)	148, 729, 818
BseJI	GATNNNNATC	2 cut(s)	82, 840
BseLI	CCNNNNNNNGG	1 cut(s)	448
BseNI	ACTGG	2 cut(s)	236, 604
BseXI	GCAGC	4 cut(s)	285, 427, 719, 942
BshFI	GGCC	1 cut(s)	723
BshVI	ATCGAT	2 cut(s)	76, 562
BsiHKAI	GWGCWC	1 cut(s)	857
BsiSI	CCGG	1 cut(s)	733
BslI	CCNNNNNNNGG	1 cut(s)	448
BsmAI	GTCTC	4 cut(s)	431, 449, 669, 694
BsmI	GAATGC	1 cut(s)	594
BsnI	GGCC	1 cut(s)	723
Bso31I	GGTCTC	1 cut(s)	431
Bsp1286I	GDGCHC	2 cut(s)	116, 857
Bsp143I	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
BspACI	CCGC	4 cut(s)	649, 743, 927, 933
BspANI	GGCC	1 cut(s)	723
BspDI	ATCGAT	2 cut(s)	76, 562
BspHI	TCATGA	2 cut(s)	40, 787
BspLI	GGNNCC	3 cut(s)	106, 234, 468
BspPI	GGATC	2 cut(s)	11, 792
BspTNI	GGTCTC	1 cut(s)	431
BsrI	ACTGG	2 cut(s)	236, 604
BssECI	CCNNGG	1 cut(s)	217
BssMI	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
BssSI	CACGAG	1 cut(s)	879
Bst2BI	CACGAG	1 cut(s)	879
Bst2UI	CCWGG	1 cut(s)	810
Bst4CI	ACNGT	3 cut(s)	482, 497, 822
BstBAI	YACGTR	1 cut(s)	833
BstC8I	GCNNGC	1 cut(s)	630
BstDEI	CTNAG	1 cut(s)	890
BstDSI	CCRYGG	1 cut(s)	217
BstF5I	GGATG	3 cut(s)	148, 729, 818
BstKTI	GATC	8 cut(s)	19, 76, 124, 229, 268, 562, 585, 800
BstMAI	GTCTC	4 cut(s)	431, 449, 669, 694
BstMBI	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
BstMWI	GCNNNNNNNGC	3 cut(s)	53, 421, 634
BstNI	CCWGG	1 cut(s)	810
BstSCI	CCNGG	1 cut(s)	808
BstV1I	GCAGC	4 cut(s)	285, 427, 719, 942
BstV2I	GAAGAC	1 cut(s)	505
Bsu15I	ATCGAT	2 cut(s)	76, 562
Bsu36I	CCTNAGG	1 cut(s)	890
BsuI	GTATCC	2 cut(s)	646, 810
BsuRI	GGCC	1 cut(s)	723
BsuTUI	ATCGAT	2 cut(s)	76, 562
BtgI	CCRYGG	1 cut(s)	217
BtsCI	GGATG	3 cut(s)	148, 729, 818
Cac8I	GCNNGC	1 cut(s)	630
CciI	TCATGA	2 cut(s)	40, 787
ClaI	ATCGAT	2 cut(s)	76, 562
Csp6I	GTAC	2 cut(s)	243, 607
CspCI	CAANNNNNGTGG	2 cut(s)	904, 939
CviAII	CATG	5 cut(s)	41, 586, 754, 788, 862
CviQI	GTAC	2 cut(s)	243, 607
DdeI	CTNAG	1 cut(s)	890
DpnI	GATC	8 cut(s)	18, 75, 123, 228, 267, 561, 584, 799
DpnII	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
Eco24I	GRGCYC	1 cut(s)	116
Eco31I	GGTCTC	1 cut(s)	431
Eco57I	CTGAAG	1 cut(s)	564
Eco81I	CCTNAGG	1 cut(s)	890
EcoRII	CCWGG	1 cut(s)	808
EcoT38I	GRGCYC	1 cut(s)	116
FaeI	CATG	5 cut(s)	44, 589, 757, 791, 865
FatI	CATG	5 cut(s)	40, 585, 753, 787, 861
FauI	CCCGC	1 cut(s)	642
FauNDI	CATATG	2 cut(s)	197, 910
FbaI	TGATCA	2 cut(s)	226, 582
Fnu4HI	GCNGC	5 cut(s)	299, 416, 708, 928, 931
FokI	GGATG	3 cut(s)	135, 716, 825
FriOI	GRGCYC	1 cut(s)	116
Fsp4HI	GCNGC	5 cut(s)	299, 416, 708, 928, 931
GluI	GCNGC	5 cut(s)	299, 416, 708, 928, 931
GsuI	CTGGAG	1 cut(s)	323
HaeIII	GGCC	1 cut(s)	723
HapII	CCGG	1 cut(s)	733
Hin1II	CATG	5 cut(s)	44, 589, 757, 791, 865
HindIII	AAGCTT	1 cut(s)	422
HinfI	GANTC	1 cut(s)	726
HpaII	CCGG	1 cut(s)	733
HphI	GGTGA	2 cut(s)	205, 592
Hpy166II	GTNNAC	1 cut(s)	609
Hpy188I	TCNGA	1 cut(s)	846
Hpy188III	TCNNGA	7 cut(s)	14, 41, 102, 323, 436, 470, 788
Hpy8I	GTNNAC	1 cut(s)	609
HpyAV	CCTTC	2 cut(s)	397, 539
HpyCH4III	ACNGT	3 cut(s)	482, 497, 822
HpyCH4IV	ACGT	1 cut(s)	832
HpyCH4V	TGCA	5 cut(s)	195, 415, 632, 839, 871
HpyF10VI	GCNNNNNNNGC	3 cut(s)	53, 421, 634
HpyF3I	CTNAG	1 cut(s)	890
HpySE526I	ACGT	1 cut(s)	832
Hsp92II	CATG	5 cut(s)	44, 589, 757, 791, 865
Ksp22I	TGATCA	2 cut(s)	226, 582
Kzo9I	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
LmnI	GCTCC	1 cut(s)	472
Lsp1109I	GCAGC	4 cut(s)	285, 427, 719, 942
LweI	GCATC	3 cut(s)	34, 682, 826
MaeII	ACGT	1 cut(s)	832
MaeIII	GTNAC	2 cut(s)	350, 491
MalI	GATC	8 cut(s)	18, 75, 123, 228, 267, 561, 584, 799
MboI	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
MboII	GAAGA	4 cut(s)	128, 505, 769, 907
MfeI	CAATTG	1 cut(s)	117
MhlI	GDGCHC	2 cut(s)	116, 857
MluCI	AATT	5 cut(s)	117, 142, 396, 475, 803
MlyI	GAGTC	1 cut(s)	735
MnlI	CCTC	4 cut(s)	285, 495, 524, 664
MseI	TTAA	3 cut(s)	141, 182, 614
MslI	CAYNNNNRTG	2 cut(s)	831, 860
MspA1I	CMGCKG	2 cut(s)	637, 933
MspI	CCGG	1 cut(s)	733
MspR9I	CCNGG	1 cut(s)	810
MunI	CAATTG	1 cut(s)	117
Mva1269I	GAATGC	1 cut(s)	594
MvaI	CCWGG	1 cut(s)	810
MwoI	GCNNNNNNNGC	3 cut(s)	53, 421, 634
NdeI	CATATG	2 cut(s)	197, 910
NdeII	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
NlaIII	CATG	5 cut(s)	44, 589, 757, 791, 865
NlaIV	GGNNCC	3 cut(s)	106, 234, 468
NmeAIII	GCCGAG	1 cut(s)	749
NmuCI	GTSAC	1 cut(s)	491
PagI	TCATGA	2 cut(s)	40, 787
PctI	GAATGC	1 cut(s)	594
PflMI	CCANNNNNTGG	1 cut(s)	448
PkrI	GCNGC	5 cut(s)	300, 417, 709, 929, 932
PleI	GAGTC	1 cut(s)	734
PpsI	GAGTC	1 cut(s)	734
Ppu21I	YACGTR	1 cut(s)	833
Psp6I	CCWGG	1 cut(s)	808
PspGI	CCWGG	1 cut(s)	808
PspN4I	GGNNCC	3 cut(s)	106, 234, 468
PvuII	CAGCTG	1 cut(s)	637
RsaI	GTAC	2 cut(s)	244, 608
RsaNI	GTAC	2 cut(s)	243, 607
RseI	CAYNNNNRTG	2 cut(s)	831, 860
SaqAI	TTAA	3 cut(s)	141, 182, 614
SatI	GCNGC	5 cut(s)	299, 416, 708, 928, 931
Sau3AI	GATC	8 cut(s)	16, 73, 121, 226, 265, 559, 582, 797
SchI	GAGTC	1 cut(s)	735
ScrFI	CCNGG	1 cut(s)	810
SduI	GDGCHC	2 cut(s)	116, 857
SfaNI	GCATC	3 cut(s)	34, 682, 826
SmiMI	CAYNNNNRTG	2 cut(s)	831, 860
SmlI	CTYRAG	3 cut(s)	109, 697, 850
SmoI	CTYRAG	3 cut(s)	109, 697, 850
Sse9I	AATT	5 cut(s)	117, 142, 396, 475, 803
SsiI	CCGC	4 cut(s)	649, 743, 927, 933
StyD4I	CCNGG	1 cut(s)	808
TaaI	ACNGT	3 cut(s)	482, 497, 822
TaiI	ACGT	1 cut(s)	835
TaqI	TCGA	4 cut(s)	76, 163, 435, 562
TasI	AATT	5 cut(s)	117, 142, 396, 475, 803
TatI	WGTACW	1 cut(s)	606
TauI	GCSGC	1 cut(s)	930
Tru1I	TTAA	3 cut(s)	141, 182, 614
Tru9I	TTAA	3 cut(s)	141, 182, 614
TseFI	GTSAC	1 cut(s)	491
TseI	GCWGC	4 cut(s)	298, 415, 707, 930
Tsp45I	GTSAC	1 cut(s)	491
TspDTI	ATGAA	3 cut(s)	402, 421, 770
TspGWI	ACGGA	1 cut(s)	872
Van91I	CCANNNNNTGG	1 cut(s)	448
XapI	RAATTY	1 cut(s)	396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.