AT1G04850

PUB domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008996
Physical Location & Seq
Reverse (-)
1365107 .. 1368940
3834 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04850.2

Sequence Viewer

Length: 1242 bp
ATGGCAGGAGTATCGCTCAAGTGTGGCGATTGTGGGACGCTGCTGAAGTCCGTGGAGGAGGCTCAGGAGCACGCTGAGCTCACCTCTCACTCCAATTTCGCCGAGTCCACTGAGGCCGTGCTCAATCTCGTTTGTACTACTTGCACCAAGCCTTGTCGCTCAAAAATCGAAAGCGATTTGCACACGAAAAGAACAGGGCATACTGAGTTTGTAGATAAGACATTGGAGACAATCAAACCCATCAGCTTGGAAGCTCCTAAGGTTGCCATGGAGATTGATGATAATGCTAGTGGTAGTGGAGAGGCTGCAGAAGAGATGGTTGTTCCAGACGTTGACAATAACATTCTCGAGGAACTTGAAGCGATGGGCTTCCCTAAAGCTCGTGCTACCCGAGCACTCCATTACTCTGGTAATGCCAGTCTTGAGGCTGCAGTTAATTGGGTGGTGGAGCATGAGAATGATCCTGACGTAGATGAAATGCCAAAGGTGCCTTCCAACTCCAATGTTGGGCCTGCCAAACCTGCTCTTACGCCAGAAGAAGTGAAGCTAAAAGCACAAGAATTAAGGGAACGTGCACGGAAGAAGAAAGAAGAGGAAGAAAAACGGATGGAACGTGAAAGAGAGAAGGAGCGAATTAGGATTGGCAAGGAACTCTTAGAAGCAAAGCGGATGGAAGAAGTAAACGAAAGAAAACGTCTTATGTTTTTGCGTAAAGCTGAGAAAGAAGAAGAAAAACGGGCTAGGGAAAAGATCCGTCAAAAGCTTGAAGAAGACAAGGCTGAAAGAAGGCGAAAACTCGGATTGCCTCCGGAAGATCCTGCAACTGCAGCTGCAAAACCCTCAGTGCCAGTTGTGGAGGAGAAGAAGGTTACATTGCCAATTAGACCGGCCACAAAGACTGAACAAATGAGAGAATGCTTGAGGTCCCTTAAGCAAGCCCACAAGGAGGATGATGCTAAAGTGAAGAGAGCGTTCCAGACTCTGCTGACTTACATGGGAAATGTCGCTAAGAATCCAGATGAGGAAAAATTCCGGAAAATTAGACTCACCAACCAAACATTTCAGGAAAGGGTTGGTTCGCTAAGAGGGGGCATAGAGTTCATGGAGCTATGTGGATTTGAGAAAATTGAAGGAGGAGAGTTCTTGTTCTTACCTAGAGACAAGATCGATTCAGCCATCATAAACTCAGCTGGAACAGAACTCAACTCCGCCATCAACAACCCTTTCTTTGGAGTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUB UBA

Protein Analysis

413

Amino Acids

46.6

Weight (kDa)

5.91

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_7 PF22562 23 - 70 2.3e-18 UBA-like domain
UBA PF00627 23 - 58 8.6e-09 UBA/TS-N domain
PUB PF09409 231 - 302 6.4e-25 PUB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 1 cut(s) 487
AccIII TCCGGA 2 cut(s) 808, 1032
AciI CCGC 2 cut(s) 667, 1209
AclWI GGATC 3 cut(s) 455, 745, 809
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 1 cut(s) 1028
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 3 cut(s) 507, 946, 1229
AflII CTTAAG 1 cut(s) 929
AgsI TTSAA 3 cut(s) 359, 767, 1130
AjuI GAANNNNNNNTTGG 2 cut(s) 475, 507
Alw21I GWGCWC 5 cut(s) 72, 81, 123, 397, 577
Alw26I GTCTC 2 cut(s) 221, 1152
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 3 cut(s) 455, 745, 809
AlwNI CAGNNNCTG 1 cut(s) 982
Ama87I CYCGRG 2 cut(s) 347, 390
Aor13HI TCCGGA 2 cut(s) 808, 1032
AoxI GGCC 3 cut(s) 114, 509, 888
ApaLI GTGCAC 1 cut(s) 573
ApeKI GCWGC 5 cut(s) 40, 305, 428, 827, 830
ApoI RAATTY 1 cut(s) 1028
AspS9I GGNCC 2 cut(s) 509, 924
AsuHPI GGTGA 2 cut(s) 73, 1039
AvaI CYCGRG 2 cut(s) 347, 390
AvaII GGWCC 1 cut(s) 924
AxyI CCTNAGG 1 cut(s) 258
BaeGI GKGCMC 1 cut(s) 577
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 81
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 777
Bbv12I GWGCWC 5 cut(s) 72, 81, 123, 397, 577
BbvI GCAGC 5 cut(s) 27, 292, 415, 817, 839
BccI CCATC 7 cut(s) 248, 310, 358, 601, 664, 1184, 1220
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 1 cut(s) 28
BcoDI GTCTC 2 cut(s) 221, 1152
BfaI CTAG 3 cut(s) 288, 741, 1155
BfmI CTRYAG 3 cut(s) 306, 429, 825
BfrI CTTAAG 1 cut(s) 929
BfuAI ACCTGC 1 cut(s) 529
BisI GCNGC 5 cut(s) 41, 306, 429, 828, 831
BlpI GCTNAGC 1 cut(s) 75
BlsI GCNGC 5 cut(s) 42, 307, 430, 829, 832
Bme18I GGWCC 1 cut(s) 924
BmeT110I CYCGRG 2 cut(s) 347, 390
BmgT120I GGNCC 2 cut(s) 509, 924
BmiI GGNNCC 2 cut(s) 489, 926
BmsI GCATC 1 cut(s) 943
BpiI GAAGAC 1 cut(s) 777
BplI GAGNNNNNCTC 3 cut(s) 32, 68, 100
Bpu10I CCTNAGC 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 75
BpuEI CTTGAG 2 cut(s) 443, 940
Bsa29I ATCGAT 1 cut(s) 1167
BsaJI CCNNGG 2 cut(s) 51, 267
BsaWI WCCGGW 2 cut(s) 808, 1032
Bsc4I CCNNNNNNNGG 3 cut(s) 507, 946, 1229
Bse118I RCCGGY 1 cut(s) 886
Bse1I ACTGG 2 cut(s) 417, 848
Bse21I CCTNAGG 1 cut(s) 258
Bse3DI GCAATG 1 cut(s) 872
BseAI TCCGGA 2 cut(s) 808, 1032
BseCI ATCGAT 1 cut(s) 1167
BseDI CCNNGG 2 cut(s) 51, 267
BseGI GGATG 3 cut(s) 612, 675, 955
BseLI CCNNNNNNNGG 3 cut(s) 507, 946, 1229
BseMI GCAATG 1 cut(s) 872
BseMII CTCAG 7 cut(s) 66, 77, 102, 195, 708, 855, 1200
BseNI ACTGG 2 cut(s) 417, 848
BseRI GAGGAG 3 cut(s) 71, 872, 1149
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 5 cut(s) 27, 292, 415, 817, 839
BshFI GGCC 3 cut(s) 116, 511, 890
BshNI GGYRCC 1 cut(s) 487
BshVI ATCGAT 1 cut(s) 1167
BsiHKAI GWGCWC 5 cut(s) 72, 81, 123, 397, 577
BsiHKCI CYCGRG 2 cut(s) 347, 390
BsiSI CCGG 3 cut(s) 809, 887, 1033
BslFI GGGAC 2 cut(s) 49, 910
BslI CCNNNNNNNGG 3 cut(s) 507, 946, 1229
BsmAI GTCTC 2 cut(s) 221, 1152
BsmFI GGGAC 2 cut(s) 49, 910
BsmI GAATGC 1 cut(s) 920
BsnI GGCC 3 cut(s) 116, 511, 890
BsoBI CYCGRG 2 cut(s) 347, 390
Bsp1286I GDGCHC 5 cut(s) 72, 81, 123, 397, 577
Bsp13I TCCGGA 2 cut(s) 808, 1032
Bsp143I GATC 4 cut(s) 460, 750, 814, 1164
Bsp1720I GCTNAGC 1 cut(s) 75
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 2 cut(s) 667, 1209
BspANI GGCC 3 cut(s) 116, 511, 890
BspCNI CTCAG 7 cut(s) 67, 76, 103, 196, 709, 854, 1199
BspDI ATCGAT 1 cut(s) 1167
BspEI TCCGGA 2 cut(s) 808, 1032
BspLI GGNNCC 2 cut(s) 489, 926
BspMAI CTGCAG 3 cut(s) 310, 433, 829
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 3 cut(s) 455, 745, 809
BspT107I GGYRCC 1 cut(s) 487
BspTI CTTAAG 1 cut(s) 929
BsrDI GCAATG 1 cut(s) 872
BsrFI RCCGGY 1 cut(s) 886
BsrI ACTGG 2 cut(s) 417, 848
BssAI RCCGGY 1 cut(s) 886
BssECI CCNNGG 2 cut(s) 51, 267
BssMI GATC 4 cut(s) 460, 750, 814, 1164
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 267
Bst2BI CACGAG 1 cut(s) 381
Bst6I CTCTTC 3 cut(s) 306, 585, 959
BstAFI CTTAAG 1 cut(s) 929
BstC8I GCNNGC 3 cut(s) 72, 513, 936
BstDSI CCRYGG 2 cut(s) 51, 267
BstF5I GGATG 3 cut(s) 612, 675, 955
BstKTI GATC 4 cut(s) 463, 753, 817, 1167
BstMAI GTCTC 2 cut(s) 221, 1152
BstMBI GATC 4 cut(s) 460, 750, 814, 1164
BstMWI GCNNNNNNNGC 5 cut(s) 76, 392, 487, 521, 827
BstSFI CTRYAG 3 cut(s) 306, 429, 825
BstSLI GKGCMC 1 cut(s) 577
BstV1I GCAGC 5 cut(s) 27, 292, 415, 817, 839
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 2 cut(s) 750, 814
BstXI CCANNNNNNTGG 2 cut(s) 247, 407
BstYI RGATCY 2 cut(s) 750, 814
Bsu15I ATCGAT 1 cut(s) 1167
Bsu36I CCTNAGG 1 cut(s) 258
BsuRI GGCC 3 cut(s) 116, 511, 890
BsuTUI ATCGAT 1 cut(s) 1167
BtgI CCRYGG 2 cut(s) 51, 267
BtgZI GCGATG 1 cut(s) 377
BtsCI GGATG 3 cut(s) 612, 675, 955
BtsIMutI CAGTG 2 cut(s) 108, 849
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 3 cut(s) 72, 513, 936
CaiI CAGNNNCTG 1 cut(s) 982
Cfr10I RCCGGY 1 cut(s) 886
Cfr13I GGNCC 2 cut(s) 509, 924
ClaI ATCGAT 1 cut(s) 1167
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 135
CviAII CATG 4 cut(s) 268, 452, 994, 1102
CviQI GTAC 1 cut(s) 135
DpnI GATC 4 cut(s) 462, 752, 816, 1166
DpnII GATC 4 cut(s) 460, 750, 814, 1164
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 3 cut(s) 306, 585, 959
EarI CTCTTC 3 cut(s) 306, 585, 959
EciI GGCGGA 1 cut(s) 1198
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 924
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 258
Eco88I CYCGRG 2 cut(s) 347, 390
EcoICRI GAGCTC 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 924
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 4 cut(s) 271, 455, 997, 1105
FaiI YATR 9 cut(s) 201, 269, 453, 701, 995, 1094, 1103, 1111, 1181
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 2 cut(s) 49, 910
FatI CATG 4 cut(s) 267, 451, 993, 1101
Fnu4HI GCNGC 5 cut(s) 41, 306, 429, 828, 831
FokI GGATG 3 cut(s) 619, 682, 962
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 5 cut(s) 41, 306, 429, 828, 831
FspBI CTAG 3 cut(s) 288, 741, 1155
GluI GCNGC 5 cut(s) 41, 306, 429, 828, 831
HaeIII GGCC 3 cut(s) 116, 511, 890
HapII CCGG 3 cut(s) 809, 887, 1033
HgaI GACGC 1 cut(s) 46
Hin1II CATG 4 cut(s) 271, 455, 997, 1105
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 5 cut(s) 104, 979, 1012, 1044, 1169
HpaII CCGG 3 cut(s) 809, 887, 1033
HphI GGTGA 2 cut(s) 73, 1039
Hpy166II GTNNAC 4 cut(s) 108, 334, 575, 682
Hpy188I TCNGA 1 cut(s) 800
Hpy8I GTNNAC 4 cut(s) 108, 334, 575, 682
HpyAV CCTTC 5 cut(s) 501, 619, 780, 859, 1124
HpyCH4IV ACGT 5 cut(s) 330, 468, 571, 613, 694
HpyCH4V TGCA 8 cut(s) 144, 181, 308, 431, 575, 821, 827, 833
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 392, 487, 521, 827
HpySE526I ACGT 5 cut(s) 330, 468, 571, 613, 694
Hsp92II CATG 4 cut(s) 271, 455, 997, 1105
Kpn2I TCCGGA 2 cut(s) 808, 1032
Kzo9I GATC 4 cut(s) 460, 750, 814, 1164
LmnI GCTCC 5 cut(s) 67, 259, 448, 628, 1105
Lsp1109I GCAGC 5 cut(s) 27, 292, 415, 817, 839
LweI GCATC 1 cut(s) 943
MaeI CTAG 3 cut(s) 288, 741, 1155
MaeII ACGT 5 cut(s) 330, 468, 571, 613, 694
MaeIII GTNAC 1 cut(s) 868
MalI GATC 4 cut(s) 462, 752, 816, 1166
MboI GATC 4 cut(s) 460, 750, 814, 1164
MflI RGATCY 2 cut(s) 750, 814
MhlI GDGCHC 5 cut(s) 72, 81, 123, 397, 577
MluCI AATT 8 cut(s) 94, 436, 560, 633, 879, 1028, 1038, 1125
MlyI GAGTC 3 cut(s) 113, 973, 1038
MmeI TCCRAC 1 cut(s) 519
MroI TCCGGA 2 cut(s) 808, 1032
MseI TTAA 4 cut(s) 435, 563, 930, 1240
MslI CAYNNNNRTG 1 cut(s) 456
MspA1I CMGCKG 2 cut(s) 830, 1190
MspCI CTTAAG 1 cut(s) 929
MspI CCGG 3 cut(s) 809, 887, 1033
Mva1269I GAATGC 1 cut(s) 920
MwoI GCNNNNNNNGC 5 cut(s) 76, 392, 487, 521, 827
NcoI CCATGG 1 cut(s) 267
NdeII GATC 4 cut(s) 460, 750, 814, 1164
NlaIII CATG 4 cut(s) 271, 455, 997, 1105
NlaIV GGNNCC 2 cut(s) 489, 926
NmeAIII GCCGAG 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 347
PcsI WCGNNNNNNNCGW 2 cut(s) 388, 610
PctI GAATGC 1 cut(s) 920
PfeI GAWTC 2 cut(s) 1012, 1169
PkrI GCNGC 5 cut(s) 42, 307, 430, 829, 832
PleI GAGTC 3 cut(s) 112, 973, 1038
PpsI GAGTC 3 cut(s) 112, 973, 1038
PpuMI RGGWCCY 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 924
PspN4I GGNNCC 2 cut(s) 489, 926
PspPI GGNCC 2 cut(s) 509, 924
PspPPI RGGWCCY 1 cut(s) 924
PsrI GAACNNNNNNTAC 2 cut(s) 184, 216
PstI CTGCAG 3 cut(s) 310, 433, 829
PstNI CAGNNNCTG 1 cut(s) 982
PsuI RGATCY 2 cut(s) 750, 814
PvuII CAGCTG 2 cut(s) 830, 1190
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 456
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 4 cut(s) 435, 563, 930, 1240
SatI GCNGC 5 cut(s) 41, 306, 429, 828, 831
Sau3AI GATC 4 cut(s) 460, 750, 814, 1164
Sau96I GGNCC 2 cut(s) 509, 924
SchI GAGTC 3 cut(s) 113, 973, 1038
SduI GDGCHC 5 cut(s) 72, 81, 123, 397, 577
SfaNI GCATC 1 cut(s) 943
SfcI CTRYAG 3 cut(s) 306, 429, 825
Sfr274I CTCGAG 1 cut(s) 347
SinI GGWCC 1 cut(s) 924
SlaI CTCGAG 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 5 cut(s) 17, 347, 422, 919, 929
SmoI CTYRAG 5 cut(s) 17, 347, 422, 919, 929
Sse9I AATT 8 cut(s) 94, 436, 560, 633, 879, 1028, 1038, 1125
SsiI CCGC 2 cut(s) 667, 1209
SspMI CTAG 3 cut(s) 288, 741, 1155
SstI GAGCTC 1 cut(s) 81
StyI CCWWGG 1 cut(s) 267
TaiI ACGT 5 cut(s) 333, 471, 574, 616, 697
TaqI TCGA 3 cut(s) 168, 348, 1167
TasI AATT 8 cut(s) 94, 436, 560, 633, 879, 1028, 1038, 1125
TatI WGTACW 1 cut(s) 134
TfiI GAWTC 2 cut(s) 1012, 1169
Tru1I TTAA 4 cut(s) 435, 563, 930, 1240
Tru9I TTAA 4 cut(s) 435, 563, 930, 1240
TscAI CASTG 2 cut(s) 115, 849
TseI GCWGC 5 cut(s) 40, 305, 428, 827, 830
TspDTI ATGAA 2 cut(s) 489, 1090
TspGWI ACGGA 4 cut(s) 40, 592, 619, 743
TspRI CASTG 2 cut(s) 115, 849
Vha464I CTTAAG 1 cut(s) 929
VneI GTGCAC 1 cut(s) 573
VpaK11BI GGWCC 1 cut(s) 924
XapI RAATTY 1 cut(s) 1028
XhoI CTCGAG 1 cut(s) 347
XspI CTAG 3 cut(s) 288, 741, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.