AT1G05350

Ubiquitin-like modifier-activating enzyme 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010013
Physical Location & Seq
Reverse (-)
1560678 .. 1564409
3732 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05350.1

Sequence Viewer

Length: 1296 bp
ATGGAGGTTGGATTCAAGGCATTGCTCGATGATCTTGACGTTCTCGAGAAATCTCTCTCAGATCCGGCTCTGATCAACAAGCTGCGATCACATGTTGAGAATCTAGCCACTCTGTCTAAATGTAATCCTCACCGGCGTTCCAAAGTCAAGGAACTAAGCTCGGAAGTGGTAGATAGTAATCCTTACAGTAGGCTTATGGCACTTCAGCGGATGGGCATTGTGGACAACTATGAGAGAATTAGAGAGTTTTCCGTCGCCATTGTTGGAATTGGTGGTGTTGGAAGTGTTGCGGCTGAGATGCTTACCAGATGTGGTATTGGTCGTCTTTTGTTGTATGACTATGACACTGTCGAACTAGCTAACATGAACAGACTGTTTTTTAGACCGGACCAGGTTGGTATGACAAAGACTGATGCTGCTGTCCAAACCCTTGCAGAAATAAACCCTGACGTAGTTCTTGAAAGTTTCACAATGAACATAACAACAGTGCAAGGCTTTGAAACCTTCACGTCCAGCTTGACAAACAAGTCATTTTGTCCTAGCAAAGAGGGCGGCAGTGGTGTGGATCTTGTTTTGAGTTGTGTCGATAACTATGAAGCAAGGATGGCTGTCAACCAGGCATGCAATGAGTTACGTCAAACATGGATGGAGTCAGGTGTATCCGAAGATGCTGTTTCAGGTCACATACAGCTCTTGGTTCCCGGAGAAACTGCTTGTTTTGCTTGTGCACCTCCCCTGGTCGTGGCATCTGGAATTGATGAACGGACACTTAAACGGGAAGGGGTCTGTGCTGCATCTCTGCCAACTACTATGGGTGTTGTTGCGGGGCTTTTGGTTCAAAACTCTCTCAAGTTCTTACTAAATTTTGGTGAAGTTTCTCCATACTTGGGTTATAATTCACTGAAGGATTTTTTCCCAACCATGAAAATGAGACCAAATCCACAATGCTCGAATGTAGCTTGTCTAGAGCGGCAGAAAGAATATATGCTTGCAAAACCAGAAAGGGATGCTGCTGCTAAAGCTAAGATGGAAGCTGATGCATCCACAACTATCGATGAAGGTCCACTCCATGATGATAATGAGTGGAACATAAGTGTTGTCGATGATGAAAATGAGAAGGATACTACAAAAGCTGCCTCAAGTTCAGATACATTACCCGAAGGGCTTACTCGGGAGCTTCCAGTTGCAGATGAGTATGAGAAAGCAATAGCCATAGCCTCAGGGTCAGGAGAAACAGAGGAGGAAGATGACCTTGAAGATCTGAAGAAACAGCTCGAAGCTCTCAACGCTGCTTGA

Protein Analysis

431

Amino Acids

47.02

Weight (kDa)

4.58

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 62 - 317 1.4e-57 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 894
AasI GACNNNNNNGTC 1 cut(s) 526
AccBSI CCGCTC 1 cut(s) 970
AciI CCGC 5 cut(s) 208, 290, 552, 824, 970
AclWI GGATC 2 cut(s) 56, 573
AcsI RAATTY 1 cut(s) 862
AcuI CTGAAG 3 cut(s) 188, 923, 1283
AfiI CCNNNNNNNGG 2 cut(s) 742, 887
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 5 cut(s) 16, 461, 500, 839, 1256
AjiI CACGTC 1 cut(s) 510
AjnI CCWGG 3 cut(s) 390, 615, 735
Alw21I GWGCWC 1 cut(s) 730
Alw26I GTCTC 1 cut(s) 925
Alw44I GTGCAC 1 cut(s) 726
AlwI GGATC 2 cut(s) 56, 573
Ama87I CYCGRG 2 cut(s) 44, 1170
ApaLI GTGCAC 1 cut(s) 726
ApeKI GCWGC 7 cut(s) 82, 416, 791, 1010, 1013, 1133, 1289
ApoI RAATTY 1 cut(s) 862
AspS9I GGNCC 2 cut(s) 388, 1061
AsuC2I CCSGG 1 cut(s) 702
AsuHPI GGTGA 2 cut(s) 122, 881
AvaI CYCGRG 2 cut(s) 44, 1170
AvaII GGWCC 2 cut(s) 388, 1061
AxyI CCTNAGG 1 cut(s) 1219
BaeGI GKGCMC 1 cut(s) 730
Bbv12I GWGCWC 1 cut(s) 730
BbvI GCAGC 7 cut(s) 69, 403, 778, 997, 1000, 1120, 1276
BccI CCATC 4 cut(s) 205, 598, 640, 1021
BciT130I CCWGG 3 cut(s) 392, 617, 737
BciVI GTATCC 2 cut(s) 670, 1114
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 702
BcoDI GTCTC 1 cut(s) 925
BfaI CTAG 4 cut(s) 104, 356, 540, 965
BfuI GTATCC 2 cut(s) 670, 1114
BglII AGATCT 1 cut(s) 1258
Bme1390I CCNGG 4 cut(s) 392, 617, 702, 737
Bme18I GGWCC 2 cut(s) 388, 1061
BmeT110I CYCGRG 2 cut(s) 44, 1170
BmgBI CACGTC 1 cut(s) 510
BmgT120I GGNCC 2 cut(s) 388, 1061
BmiI GGNNCC 1 cut(s) 699
BmrFI CCNGG 4 cut(s) 392, 617, 702, 737
BmsI GCATC 8 cut(s) 288, 403, 658, 755, 803, 997, 1027, 1049
BpuEI CTTGAG 2 cut(s) 833, 1123
BpuMI CCSGG 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 1053
BsaBI GATNNNNATC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 925
BsaJI CCNNGG 1 cut(s) 735
BsaWI WCCGGW 1 cut(s) 385
Bsc4I CCNNNNNNNGG 2 cut(s) 742, 887
Bse118I RCCGGY 1 cut(s) 132
Bse1I ACTGG 1 cut(s) 1181
Bse21I CCTNAGG 1 cut(s) 1219
Bse3DI GCAATG 2 cut(s) 20, 631
Bse8I GATNNNNATC 1 cut(s) 177
BseBI CCWGG 3 cut(s) 392, 617, 737
BseCI ATCGAT 1 cut(s) 1053
BseDI CCNNGG 1 cut(s) 735
BseGI GGATG 5 cut(s) 216, 609, 651, 1012, 1040
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 742, 887
BseMI GCAATG 2 cut(s) 20, 631
BseMII CTCAG 3 cut(s) 72, 285, 1233
BseNI ACTGG 1 cut(s) 1181
BseRI GAGGAG 1 cut(s) 1253
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 7 cut(s) 69, 403, 778, 997, 1000, 1120, 1276
BshVI ATCGAT 1 cut(s) 1053
BsiHKAI GWGCWC 1 cut(s) 730
BsiHKCI CYCGRG 2 cut(s) 44, 1170
BsiSI CCGG 4 cut(s) 65, 133, 386, 702
BslI CCNNNNNNNGG 2 cut(s) 742, 887
BsmAI GTCTC 1 cut(s) 925
Bso31I GGTCTC 1 cut(s) 925
BsoBI CYCGRG 2 cut(s) 44, 1170
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
BspACI CCGC 5 cut(s) 208, 290, 552, 824, 970
BspCNI CTCAG 3 cut(s) 71, 286, 1232
BspDI ATCGAT 1 cut(s) 1053
BspLI GGNNCC 1 cut(s) 699
BspPI GGATC 2 cut(s) 56, 573
BspTNI GGTCTC 1 cut(s) 925
BsrBI CCGCTC 1 cut(s) 970
BsrDI GCAATG 2 cut(s) 20, 631
BsrFI RCCGGY 1 cut(s) 132
BsrI ACTGG 1 cut(s) 1181
BssAI RCCGGY 1 cut(s) 132
BssECI CCNNGG 1 cut(s) 735
BssMI GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
Bst2UI CCWGG 3 cut(s) 392, 617, 737
Bst4CI ACNGT 4 cut(s) 188, 349, 375, 487
BstC8I GCNNGC 2 cut(s) 622, 990
BstDEI CTNAG 5 cut(s) 58, 155, 294, 1023, 1219
BstF5I GGATG 5 cut(s) 216, 609, 651, 1012, 1040
BstKTI GATC 6 cut(s) 34, 64, 75, 89, 568, 1261
BstMAI GTCTC 1 cut(s) 925
BstMBI GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
BstMWI GCNNNNNNNGC 5 cut(s) 549, 605, 719, 1019, 1286
BstNI CCWGG 3 cut(s) 392, 617, 737
BstNSI RCATGY 2 cut(s) 95, 624
BstSCI CCNGG 4 cut(s) 390, 615, 700, 735
BstSLI GKGCMC 1 cut(s) 730
BstV1I GCAGC 7 cut(s) 69, 403, 778, 997, 1000, 1120, 1276
BstX2I RGATCY 3 cut(s) 61, 565, 1258
BstYI RGATCY 3 cut(s) 61, 565, 1258
Bsu15I ATCGAT 1 cut(s) 1053
Bsu36I CCTNAGG 1 cut(s) 1219
BsuI GTATCC 2 cut(s) 670, 1114
BsuTUI ATCGAT 1 cut(s) 1053
BtrI CACGTC 1 cut(s) 510
BtsCI GGATG 5 cut(s) 216, 609, 651, 1012, 1040
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 4 cut(s) 345, 492, 562, 899
Cac8I GCNNGC 2 cut(s) 622, 990
Cfr10I RCCGGY 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 388, 1061
ClaI ATCGAT 1 cut(s) 1053
CsiI ACCWGGT 1 cut(s) 390
CviAII CATG 6 cut(s) 92, 364, 621, 642, 922, 1070
DdeI CTNAG 5 cut(s) 58, 155, 294, 1023, 1219
DpnI GATC 6 cut(s) 33, 63, 74, 88, 567, 1260
DpnII GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
DrdI GACNNNNNNGTC 1 cut(s) 526
DseDI GACNNNNNNGTC 1 cut(s) 526
Eco31I GGTCTC 1 cut(s) 925
Eco47I GGWCC 2 cut(s) 388, 1061
Eco57I CTGAAG 3 cut(s) 188, 923, 1283
Eco81I CCTNAGG 1 cut(s) 1219
Eco88I CYCGRG 2 cut(s) 44, 1170
EcoRII CCWGG 3 cut(s) 390, 615, 735
EcoT22I ATGCAT 1 cut(s) 1042
FaeI CATG 6 cut(s) 95, 367, 624, 645, 925, 1073
FalI AAGNNNNNCTT 2 cut(s) 1236, 1268
FatI CATG 6 cut(s) 91, 363, 620, 641, 921, 1069
FauI CCCGC 1 cut(s) 817
FbaI TGATCA 1 cut(s) 72
FokI GGATG 5 cut(s) 223, 616, 658, 1019, 1027
FspBI CTAG 4 cut(s) 104, 356, 540, 965
HapII CCGG 4 cut(s) 65, 133, 386, 702
Hin1II CATG 6 cut(s) 95, 367, 624, 645, 925, 1073
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HinfI GANTC 3 cut(s) 12, 100, 650
HpaII CCGG 4 cut(s) 65, 133, 386, 702
HphI GGTGA 2 cut(s) 122, 881
Hpy166II GTNNAC 4 cut(s) 223, 613, 728, 1064
Hpy188I TCNGA 6 cut(s) 61, 72, 163, 664, 1147, 1263
Hpy188III TCNNGA 8 cut(s) 35, 44, 46, 458, 750, 965, 1172, 1227
Hpy8I GTNNAC 4 cut(s) 223, 613, 728, 1064
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 6 cut(s) 514, 773, 898, 1052, 1111, 1154
HpyCH4III ACNGT 4 cut(s) 188, 349, 375, 487
HpyCH4IV ACGT 4 cut(s) 39, 450, 509, 634
HpyCH4V TGCA 8 cut(s) 434, 490, 624, 728, 794, 992, 1040, 1187
HpyF10VI GCNNNNNNNGC 5 cut(s) 549, 605, 719, 1019, 1286
HpyF3I CTNAG 5 cut(s) 58, 155, 294, 1023, 1219
HpySE526I ACGT 4 cut(s) 39, 450, 509, 634
Hsp92II CATG 6 cut(s) 95, 367, 624, 645, 925, 1073
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
LmnI GCTCC 1 cut(s) 1174
Lsp1109I GCAGC 7 cut(s) 69, 403, 778, 997, 1000, 1120, 1276
LweI GCATC 8 cut(s) 288, 403, 658, 755, 803, 997, 1027, 1049
MabI ACCWGGT 1 cut(s) 390
MaeI CTAG 4 cut(s) 104, 356, 540, 965
MaeII ACGT 4 cut(s) 39, 450, 509, 634
MaeIII GTNAC 2 cut(s) 630, 680
MalI GATC 6 cut(s) 33, 63, 74, 88, 567, 1260
MbiI CCGCTC 1 cut(s) 970
MboI GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
MboII GAAGA 4 cut(s) 677, 1256, 1268, 1276
MflI RGATCY 3 cut(s) 61, 565, 1258
MhlI GDGCHC 1 cut(s) 730
MluCI AATT 5 cut(s) 237, 267, 753, 862, 895
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 2 cut(s) 244, 259
MnlI CCTC 7 cut(s) 138, 541, 741, 1147, 1228, 1231, 1234
Mph1103I ATGCAT 1 cut(s) 1042
MseI TTAA 1 cut(s) 771
MslI CAYNNNNRTG 1 cut(s) 926
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 4 cut(s) 65, 133, 386, 702
MspR9I CCNGG 4 cut(s) 392, 617, 702, 737
MvaI CCWGG 3 cut(s) 392, 617, 737
MwoI GCNNNNNNNGC 5 cut(s) 549, 605, 719, 1019, 1286
NciI CCSGG 1 cut(s) 702
NdeII GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
NlaIII CATG 6 cut(s) 95, 367, 624, 645, 925, 1073
NlaIV GGNNCC 1 cut(s) 699
NmuCI GTSAC 1 cut(s) 680
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 2 cut(s) 95, 624
PaeI GCATGC 1 cut(s) 624
PaeR7I CTCGAG 1 cut(s) 44
PciI ACATGT 1 cut(s) 91
PfeI GAWTC 2 cut(s) 12, 100
PflFI GACNNNGTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 700
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
PscI ACATGT 1 cut(s) 91
PsiI TTATAA 1 cut(s) 894
Psp6I CCWGG 3 cut(s) 390, 615, 735
PspGI CCWGG 3 cut(s) 390, 615, 735
PspN4I GGNNCC 1 cut(s) 699
PspPI GGNCC 2 cut(s) 388, 1061
PsuI RGATCY 3 cut(s) 61, 565, 1258
PsyI GACNNNGTC 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 926
SaqAI TTAA 1 cut(s) 771
Sau3AI GATC 6 cut(s) 31, 61, 72, 86, 565, 1258
Sau96I GGNCC 2 cut(s) 388, 1061
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 4 cut(s) 392, 617, 702, 737
SduI GDGCHC 1 cut(s) 730
SexAI ACCWGGT 1 cut(s) 390
SfaNI GCATC 8 cut(s) 288, 403, 658, 755, 803, 997, 1027, 1049
Sfr274I CTCGAG 1 cut(s) 44
SgrAI CRCCGGYG 1 cut(s) 132
SinI GGWCC 2 cut(s) 388, 1061
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 926
SmlI CTYRAG 3 cut(s) 44, 848, 1138
SmoI CTYRAG 3 cut(s) 44, 848, 1138
SphI GCATGC 1 cut(s) 624
Sse9I AATT 5 cut(s) 237, 267, 753, 862, 895
SsiI CCGC 5 cut(s) 208, 290, 552, 824, 970
SspMI CTAG 4 cut(s) 104, 356, 540, 965
StyD4I CCNGG 4 cut(s) 390, 615, 700, 735
TaaI ACNGT 4 cut(s) 188, 349, 375, 487
TaiI ACGT 4 cut(s) 42, 453, 512, 637
TaqI TCGA 8 cut(s) 27, 45, 351, 585, 950, 1053, 1101, 1275
TasI AATT 5 cut(s) 237, 267, 753, 862, 895
TauI GCSGC 3 cut(s) 293, 555, 973
TfiI GAWTC 2 cut(s) 12, 100
Tru1I TTAA 1 cut(s) 771
Tru9I TTAA 1 cut(s) 771
TscAI CASTG 4 cut(s) 352, 492, 562, 906
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 7 cut(s) 82, 416, 791, 1010, 1013, 1133, 1289
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 7 cut(s) 380, 488, 609, 774, 938, 1071, 1122
TspGWI ACGGA 2 cut(s) 241, 778
TspRI CASTG 4 cut(s) 352, 492, 562, 906
Tth111I GACNNNGTC 1 cut(s) 347
VneI GTGCAC 1 cut(s) 726
VpaK11BI GGWCC 2 cut(s) 388, 1061
XapI RAATTY 1 cut(s) 862
XbaI TCTAGA 1 cut(s) 964
XceI RCATGY 2 cut(s) 95, 624
XhoI CTCGAG 1 cut(s) 44
XspI CTAG 4 cut(s) 104, 356, 540, 965
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.