MD14G1157100.v1.1

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010013
Physical Location & Seq
Forward (+)
25132105 .. 25136313
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1157100.v1.1.491

Sequence Viewer

Length: 1470 bp
ATGGCCCAATTTTTCGGGGCCCAAATTGTTGGTCCACTCTCGGTATCACAGGCTCACAGACCAGCTCCGGCAAAGTGGCAAACAACAGTGACACCATTTCCTTCGCTGCCGCAAGGCAGGTACCGGTTCCCGAATTTAAGAGCGTTTTTTAGAGAGAGAGAGAGAGAGAGAGAGAGAGGAGAGAGAGAGGCAATGGAGGTGGAACTGAAGGAGTTGTTGAGCGACCTTGAATCGCTGAAGAAATCGCTGGCCGATCCTTCTCATCATGCTCCCATCGATAAGCTACAATTGCGTGTAGAACGCCTTACGAGCCTTGCAAATTCTGGACCTGTTCGGCGTTCAAAAGTGAAGGATATGAGTGCCGAGGTGGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAGAGGATGGGTATTGTGGAAAATTATGAAAGAATACGGGACTTCTCAGTTGCCATTGTTGGAGTAGGCGGTGTAGGAAGTGTAGCAGCTGAGATGCTAACAAGATGTGGTATTGGTCGCCTTTTGTTGTATGATTATGACAAAGTAGAGTTAGCTAACATGAATAGGTTATTCTTTCGACCAGAGCAGAGTGGTATGACGAAAACAGATGCTGCTGTACAGACCCTTTCAGACATAAATCCCGATGTTGTGCTTGAGAGCTATACACTGAACATCACAACTGTGCAAGGTTTTGATACCTTTATGGCCAGTTTAAAAAATAAATCGTTTCGCCCAAATAAAGAAGGCAGTGGAGTGGACCTTGTACTAAGCTGTGTTGATAATTATGAAGCAAGAATGGCTGTTAATCAGGCTTGCAATGAACTGAATCAGACATGGATGGAGTCTGGTGTGTCTGAGGATGCTGTTTCAGGCCATATTCAGTTGCTGATCCCTGGTGAGACTGCCTGTTTTGCATGTGCACCACCTTTGGTTGTGGCATCTGGAGTGGATGAACGTACACTTAAGCGTGAAGGGGTGTGTGCTGCATCTTTACCTACCACTATGGGAGTTGTTGCTGGGCTTCTAGTCCAAAATACACTAAAATACTTGCTGAACTTTGGAAACGTCTCCCCATACTTGGGATATAATTCTCTTAAAGACTTCTTCCCAACTATGGAAATGAAGCCAAACCCTCAGTGTTCTAATTTTGCTTGTCTAGATCGGCAGAAAGAATACTTGCTCGTAAAGCCAGCTAGGGATGCTGCAATTAAAGCAAAGATGGAAGCTGAAGCATCATCAGTTCCGGAATCTCCACTTCATCTTGATAATGAATGGGAAATAAGTGTTGTTGATGATAACGAGCCAGAAAGTACGGAGGCCAAAACTCCAGATGCTCTTCCAGAAGGTCTTGTTCGCGAGCTTCCCAGTGCAGATGAGTTCCAAAAACCGCCTACTGAAGCTACAGAGGGTACAGTTGACGACCTAGAAGATCTCCGAAAGCAACTTGAGGCCCTAAATTCTTGA

Protein Analysis

490

Amino Acids

54.11

Weight (kDa)

4.99

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 129 - 383 9.6e-56 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 108
Acc65I GGTACC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 1 cut(s) 1362
AccIII TCCGGA 1 cut(s) 1249
AciI CCGC 3 cut(s) 110, 474, 1394
AclWI GGATC 2 cut(s) 248, 889
AcoI YGGCCR 2 cut(s) 249, 711
AcsI RAATTY 3 cut(s) 133, 319, 1462
AcuI CTGAAG 5 cut(s) 227, 257, 389, 1254, 1422
AfaI GTAC 6 cut(s) 122, 624, 771, 964, 1318, 1417
AfiI CCNNNNNNNGG 3 cut(s) 1084, 1085, 1120
AflII CTTAAG 1 cut(s) 968
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 2 cut(s) 230, 342
AjnI CCWGG 1 cut(s) 898
AleI CACNNNNGTG 1 cut(s) 686
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 2 cut(s) 899, 1078
Alw44I GTGCAC 1 cut(s) 924
AlwI GGATC 2 cut(s) 248, 889
AlwNI CAGNNNCTG 2 cut(s) 617, 892
Aor13HI TCCGGA 1 cut(s) 1249
AoxI GGCC 7 cut(s) 3, 18, 249, 711, 877, 1323, 1455
ApaI GGGCCC 1 cut(s) 22
ApaLI GTGCAC 1 cut(s) 924
ApeKI GCWGC 5 cut(s) 106, 491, 617, 989, 1208
ApoI RAATTY 3 cut(s) 133, 319, 1462
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AsiGI ACCGGT 1 cut(s) 123
Asp718I GGTACC 1 cut(s) 120
AspS9I GGNCC 7 cut(s) 4, 18, 19, 32, 326, 763, 1456
AsuHPI GGTGA 1 cut(s) 914
AvaII GGWCC 3 cut(s) 32, 326, 763
BaeGI GKGCMC 2 cut(s) 22, 928
BalI TGGCCA 1 cut(s) 713
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 5 cut(s) 93, 503, 604, 976, 1195
BccI CCATC 4 cut(s) 281, 406, 838, 1219
BciT130I CCWGG 1 cut(s) 900
BcoDI GTCTC 2 cut(s) 899, 1078
BfaI CTAG 4 cut(s) 1031, 1163, 1200, 1430
BfmI CTRYAG 1 cut(s) 1407
BfrI CTTAAG 1 cut(s) 968
BfuAI ACCTGC 1 cut(s) 108
BglII AGATCT 1 cut(s) 1435
BisI GCNGC 6 cut(s) 107, 110, 492, 618, 990, 1209
BlsI GCNGC 6 cut(s) 108, 111, 493, 619, 991, 1210
Bme1390I CCNGG 1 cut(s) 900
Bme18I GGWCC 3 cut(s) 32, 326, 763
BmgT120I GGNCC 7 cut(s) 4, 18, 19, 32, 326, 763, 1456
BmiI GGNNCC 4 cut(s) 19, 20, 122, 128
BmrFI CCNGG 1 cut(s) 900
BmrI ACTGGG 1 cut(s) 1365
BmsI GCATC 8 cut(s) 489, 604, 856, 953, 1001, 1195, 1247, 1327
BmuI ACTGGG 1 cut(s) 1365
BpmI CTGGAG 2 cut(s) 969, 1317
BpuEI CTTGAG 1 cut(s) 680
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 363, 898
BsaWI WCCGGW 2 cut(s) 123, 1249
Bsc4I CCNNNNNNNGG 3 cut(s) 1084, 1085, 1120
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 2 cut(s) 714, 1371
Bse3DI GCAATG 2 cut(s) 198, 829
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 1 cut(s) 1249
BseBI CCWGG 1 cut(s) 900
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 363, 898
BseGI GGATG 5 cut(s) 417, 849, 871, 961, 1210
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 3 cut(s) 1084, 1085, 1120
BseMI GCAATG 2 cut(s) 198, 829
BseMII CTCAG 4 cut(s) 465, 486, 852, 1154
BseNI ACTGG 2 cut(s) 714, 1371
BseRI GAGGAG 1 cut(s) 192
BseSI GKGCMC 2 cut(s) 22, 928
BseXI GCAGC 5 cut(s) 93, 503, 604, 976, 1195
BseYI CCCAGC 1 cut(s) 1022
BsgI GTGCAG 1 cut(s) 1395
Bsh1236I CGCG 1 cut(s) 1362
BshFI GGCC 7 cut(s) 5, 20, 251, 713, 879, 1325, 1457
BshNI GGYRCC 1 cut(s) 120
BshTI ACCGGT 1 cut(s) 123
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 3 cut(s) 68, 124, 1250
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 3 cut(s) 1084, 1085, 1120
BsmAI GTCTC 2 cut(s) 899, 1078
BsmBI CGTCTC 1 cut(s) 1078
BsmFI GGGAC 1 cut(s) 458
BsnI GGCC 7 cut(s) 5, 20, 251, 713, 879, 1325, 1457
Bsp120I GGGCCC 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 22, 928
Bsp13I TCCGGA 1 cut(s) 1249
Bsp1407I TGTACA 1 cut(s) 622
Bsp143I GATC 4 cut(s) 253, 894, 1165, 1435
Bsp68I TCGCGA 1 cut(s) 1362
BspACI CCGC 3 cut(s) 110, 474, 1394
BspANI GGCC 7 cut(s) 5, 20, 251, 713, 879, 1325, 1457
BspCNI CTCAG 4 cut(s) 464, 487, 853, 1153
BspDI ATCGAT 1 cut(s) 276
BspEI TCCGGA 1 cut(s) 1249
BspFNI CGCG 1 cut(s) 1362
BspLI GGNNCC 4 cut(s) 19, 20, 122, 128
BspMI ACCTGC 1 cut(s) 108
BspPI GGATC 2 cut(s) 248, 889
BspQI GCTCTTC 1 cut(s) 1347
BspT107I GGYRCC 1 cut(s) 120
BspTI CTTAAG 1 cut(s) 968
BsrDI GCAATG 2 cut(s) 198, 829
BsrFI RCCGGY 1 cut(s) 123
BsrGI TGTACA 1 cut(s) 622
BsrI ACTGG 2 cut(s) 714, 1371
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 2 cut(s) 363, 898
BssMI GATC 4 cut(s) 253, 894, 1165, 1435
Bst2UI CCWGG 1 cut(s) 900
Bst4CI ACNGT 4 cut(s) 88, 389, 688, 1420
Bst6I CTCTTC 1 cut(s) 1347
BstAFI CTTAAG 1 cut(s) 968
BstAUI TGTACA 1 cut(s) 622
BstC8I GCNNGC 4 cut(s) 249, 820, 1197, 1364
BstDEI CTNAG 5 cut(s) 451, 495, 773, 861, 1140
BstF5I GGATG 5 cut(s) 417, 849, 871, 961, 1210
BstFNI CGCG 1 cut(s) 1362
BstKTI GATC 4 cut(s) 256, 897, 1168, 1438
BstMAI GTCTC 2 cut(s) 899, 1078
BstMBI GATC 4 cut(s) 253, 894, 1165, 1435
BstMWI GCNNNNNNNGC 7 cut(s) 289, 309, 803, 917, 1192, 1205, 1217
BstNI CCWGG 1 cut(s) 900
BstNSI RCATGY 1 cut(s) 924
BstSCI CCNGG 1 cut(s) 898
BstSFI CTRYAG 1 cut(s) 1407
BstSLI GKGCMC 2 cut(s) 22, 928
BstUI CGCG 1 cut(s) 1362
BstV1I GCAGC 5 cut(s) 93, 503, 604, 976, 1195
BstX2I RGATCY 1 cut(s) 1435
BstXI CCANNNNNNTGG 1 cut(s) 29
BstYI RGATCY 1 cut(s) 1435
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 7 cut(s) 5, 20, 251, 713, 879, 1325, 1457
BsuTUI ATCGAT 1 cut(s) 276
BtsCI GGATG 5 cut(s) 417, 849, 871, 961, 1210
BtsI GCAGTG 1 cut(s) 760
BtsIMutI CAGTG 5 cut(s) 93, 671, 760, 1148, 1378
BtuMI TCGCGA 1 cut(s) 1362
BveI ACCTGC 1 cut(s) 108
Cac8I GCNNGC 4 cut(s) 249, 820, 1197, 1364
CaiI CAGNNNCTG 2 cut(s) 617, 892
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 7 cut(s) 4, 18, 19, 32, 326, 763, 1456
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 6 cut(s) 121, 623, 770, 963, 1317, 1416
CspAI ACCGGT 1 cut(s) 123
CspCI CAANNNNNGTGG 2 cut(s) 180, 215
CviAII CATG 4 cut(s) 266, 565, 840, 921
CviQI GTAC 6 cut(s) 121, 623, 770, 963, 1317, 1416
DdeI CTNAG 5 cut(s) 451, 495, 773, 861, 1140
DpnI GATC 4 cut(s) 255, 896, 1167, 1437
DpnII GATC 4 cut(s) 253, 894, 1165, 1435
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 2 cut(s) 249, 711
Eam1104I CTCTTC 1 cut(s) 1347
EarI CTCTTC 1 cut(s) 1347
Eco24I GRGCYC 1 cut(s) 22
Eco47I GGWCC 3 cut(s) 32, 326, 763
Eco57I CTGAAG 5 cut(s) 227, 257, 389, 1254, 1422
EcoO109I RGGNCCY 2 cut(s) 18, 1456
EcoRII CCWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 22
Esp3I CGTCTC 1 cut(s) 1078
FaeI CATG 4 cut(s) 269, 568, 843, 924
FaqI GGGAC 1 cut(s) 458
FatI CATG 4 cut(s) 265, 564, 839, 920
Fnu4HI GCNGC 6 cut(s) 107, 110, 492, 618, 990, 1209
FokI GGATG 5 cut(s) 424, 856, 878, 968, 1217
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 6 cut(s) 107, 110, 492, 618, 990, 1209
FspBI CTAG 4 cut(s) 1031, 1163, 1200, 1430
GluI GCNGC 6 cut(s) 107, 110, 492, 618, 990, 1209
GsaI CCCAGC 1 cut(s) 1026
GsuI CTGGAG 2 cut(s) 969, 1317
HaeIII GGCC 7 cut(s) 5, 20, 251, 713, 879, 1325, 1457
HapII CCGG 3 cut(s) 68, 124, 1250
Hin1II CATG 4 cut(s) 269, 568, 843, 924
HincII GTYRAC 1 cut(s) 1423
HindII GTYRAC 1 cut(s) 1423
HinfI GANTC 4 cut(s) 230, 832, 848, 1253
HpaII CCGG 3 cut(s) 68, 124, 1250
HphI GGTGA 1 cut(s) 914
Hpy166II GTNNAC 5 cut(s) 35, 763, 926, 965, 1423
Hpy188I TCNGA 5 cut(s) 408, 637, 837, 862, 1442
Hpy8I GTNNAC 5 cut(s) 35, 763, 926, 965, 1423
HpyAV CCTTC 7 cut(s) 111, 202, 267, 343, 743, 971, 1343
HpyCH4III ACNGT 4 cut(s) 88, 389, 688, 1420
HpyCH4IV ACGT 2 cut(s) 961, 1071
HpyCH4V TGCA 8 cut(s) 317, 691, 822, 920, 926, 992, 1211, 1376
HpyF10VI GCNNNNNNNGC 7 cut(s) 289, 309, 803, 917, 1192, 1205, 1217
HpyF3I CTNAG 5 cut(s) 451, 495, 773, 861, 1140
HpySE526I ACGT 2 cut(s) 961, 1071
Hsp92II CATG 4 cut(s) 269, 568, 843, 924
Kpn2I TCCGGA 1 cut(s) 1249
KpnI GGTACC 1 cut(s) 124
Kzo9I GATC 4 cut(s) 253, 894, 1165, 1435
LguI GCTCTTC 1 cut(s) 1347
LmnI GCTCC 2 cut(s) 70, 274
Lsp1109I GCAGC 5 cut(s) 93, 503, 604, 976, 1195
LweI GCATC 8 cut(s) 489, 604, 856, 953, 1001, 1195, 1247, 1327
MaeI CTAG 4 cut(s) 1031, 1163, 1200, 1430
MaeII ACGT 2 cut(s) 961, 1071
MaeIII GTNAC 1 cut(s) 88
MalI GATC 4 cut(s) 255, 896, 1167, 1437
MboI GATC 4 cut(s) 253, 894, 1165, 1435
MboII GAAGA 4 cut(s) 250, 1102, 1334, 1445
MfeI CAATTG 1 cut(s) 287
MflI RGATCY 1 cut(s) 1435
MhlI GDGCHC 2 cut(s) 22, 928
MlsI TGGCCA 1 cut(s) 713
MluNI TGGCCA 1 cut(s) 713
MlyI GAGTC 1 cut(s) 857
MmeI TCCRAC 1 cut(s) 445
Mox20I TGGCCA 1 cut(s) 713
MroI TCCGGA 1 cut(s) 1249
MscI TGGCCA 1 cut(s) 713
MseI TTAA 6 cut(s) 137, 719, 810, 969, 1101, 1215
MslI CAYNNNNRTG 1 cut(s) 686
Msp20I TGGCCA 1 cut(s) 713
MspA1I CMGCKG 1 cut(s) 494
MspCI CTTAAG 1 cut(s) 968
MspI CCGG 3 cut(s) 68, 124, 1250
MspR9I CCNGG 1 cut(s) 900
MunI CAATTG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 900
MvnI CGCG 1 cut(s) 1362
MwoI GCNNNNNNNGC 7 cut(s) 289, 309, 803, 917, 1192, 1205, 1217
NdeII GATC 4 cut(s) 253, 894, 1165, 1435
NlaIII CATG 4 cut(s) 269, 568, 843, 924
NlaIV GGNNCC 4 cut(s) 19, 20, 122, 128
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 88
NruI TCGCGA 1 cut(s) 1362
NspI RCATGY 1 cut(s) 924
OliI CACNNNNGTG 1 cut(s) 686
PciSI GCTCTTC 1 cut(s) 1347
PfeI GAWTC 3 cut(s) 230, 832, 1253
PinAI ACCGGT 1 cut(s) 123
PkrI GCNGC 6 cut(s) 108, 111, 493, 619, 991, 1210
PleI GAGTC 1 cut(s) 856
PpsI GAGTC 1 cut(s) 856
Psp6I CCWGG 1 cut(s) 898
PspFI CCCAGC 1 cut(s) 1022
PspGI CCWGG 1 cut(s) 898
PspN4I GGNNCC 4 cut(s) 19, 20, 122, 128
PspOMI GGGCCC 1 cut(s) 18
PspPI GGNCC 7 cut(s) 4, 18, 19, 32, 326, 763, 1456
PstNI CAGNNNCTG 2 cut(s) 617, 892
PsuI RGATCY 1 cut(s) 1435
PvuII CAGCTG 1 cut(s) 494
RruI TCGCGA 1 cut(s) 1362
RsaI GTAC 6 cut(s) 122, 624, 771, 964, 1318, 1417
RsaNI GTAC 6 cut(s) 121, 623, 770, 963, 1317, 1416
RseI CAYNNNNRTG 1 cut(s) 686
SapI GCTCTTC 1 cut(s) 1347
SaqAI TTAA 6 cut(s) 137, 719, 810, 969, 1101, 1215
SatI GCNGC 6 cut(s) 107, 110, 492, 618, 990, 1209
Sau3AI GATC 4 cut(s) 253, 894, 1165, 1435
Sau96I GGNCC 7 cut(s) 4, 18, 19, 32, 326, 763, 1456
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 1 cut(s) 900
SduI GDGCHC 2 cut(s) 22, 928
SfaNI GCATC 8 cut(s) 489, 604, 856, 953, 1001, 1195, 1247, 1327
SfcI CTRYAG 1 cut(s) 1407
SinI GGWCC 3 cut(s) 32, 326, 763
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 3 cut(s) 659, 968, 1451
SmoI CTYRAG 3 cut(s) 659, 968, 1451
SsiI CCGC 3 cut(s) 110, 474, 1394
SspMI CTAG 4 cut(s) 1031, 1163, 1200, 1430
StyD4I CCNGG 1 cut(s) 898
TaaI ACNGT 4 cut(s) 88, 389, 688, 1420
TaiI ACGT 2 cut(s) 964, 1074
TaqI TCGA 2 cut(s) 276, 583
TatI WGTACW 2 cut(s) 622, 769
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 3 cut(s) 230, 832, 1253
Tru1I TTAA 6 cut(s) 137, 719, 810, 969, 1101, 1215
Tru9I TTAA 6 cut(s) 137, 719, 810, 969, 1101, 1215
TscAI CASTG 5 cut(s) 93, 678, 760, 1148, 1378
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 5 cut(s) 106, 491, 617, 989, 1208
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 8 cut(s) 447, 581, 807, 840, 972, 1142, 1253, 1290
TspGWI ACGGA 1 cut(s) 1334
TspRI CASTG 5 cut(s) 93, 678, 760, 1148, 1378
Vha464I CTTAAG 1 cut(s) 968
VneI GTGCAC 1 cut(s) 924
VpaK11BI GGWCC 3 cut(s) 32, 326, 763
XapI RAATTY 3 cut(s) 133, 319, 1462
XbaI TCTAGA 1 cut(s) 1162
XceI RCATGY 1 cut(s) 924
XspI CTAG 4 cut(s) 1031, 1163, 1200, 1430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.