Rw7G009380

Ubiquitin-like modifier-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010013
Physical Location & Seq
Forward (+)
8811842 .. 8815863
4022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009380.1

Sequence Viewer

Length: 1239 bp
ATGGAGGTGGAATTGAAGGAGATGCTTAACGACCTCGATTCGCTGAAGAGATCGCTCACCGATCCTTCGCAGAGCGCTCCAATCGATAAACTACAACTGCGTGTTGAACGCCTTACCAGTCTTGCAAAAACTGGACCTGTTCGGCGATCCAAAGTTCAGGATATGAGCGCGGAGGTGGTAGATAGCAATCCTTACAGTAGGCTTATGGCACTTCAGAGGATGGGCATTGTGAAAAACTATGAAAGAATAAGGGAGTTCTCCGTAGCCATAGTTGGAGTAGGTGGCGTAGGAAGTGTAGCAGCTGAGATGCTAACAAGATGTGGTATTGGTCGCCTTCTATTGTACGATTATGATAAAGTAGAGCTAGCTAACATGAATAGGTTATTCTTTCGTCCAGAGCAGAGTGGTATGACAAAAACAGATGCTGCTGTACAGACCCTTTCAGACATAAATCCTGATGTTGTGCTTGAGAGCTATACACTGAACATCACAACAGTGCAAGGTTTTGAAACCTTTACGTCCAGTTTGAAAAATAAATCATTTTGCCCAGATAAGGAAGGCAGTGGAGTAGACCTTGTGTTAAGCTGTGCTTGCAATGAACTGAATCAGACATGGATGGAGGTTTCTGAGGATGCTGTTTCAGGTCATATTCAGTTGCTTATACCTGGTGAAACTGCCTGTTTTGCATGTGCCCCACCTTTGGTTGTAGCATCTGGAATAGATGAACGCACACTTAAGCGAGAAGGGGTTTGTGCTGCATCTTTACCTACTACTATGGGAGTTGTTGCTGGGCTGCTAGTTCAGAATACACTCAAATACTTGCTGAACTTTGGAAATGTCTCCCCATACTTGGGATATAATTCTCTTAAGGACTTCTTCCCCACCATGGAAATGAGGCCAAATCCTCAGTGTTCAAATTTTGCTTGTCTGGAACGTCAGAAAGAATACTTGCTTGCAAAGCCAGCTAGGGATGCTGCTAGTAAAGCAAAGATGGAATCTGACGCATCATCATCTGTAATAGAAGCTCCACTCCATCTTGATAATGAATGGGAAATAAGCGTTGTCGATGATACTGAGCCAGATAGGATAGAGGCCAAACGTTCAGATACTCTTCCAGAAGGTCTTGTTCGGGAGCTTCCCAGTGCGGATGACTTCCAAAAACCACCTGCTGAAGCCACAGATACTACTGTTGATGACCTAGAAGAGCTTCGGAAACAACTTGAGGCCCTAAATTCTTAA

Protein Analysis

412

Amino Acids

45.38

Weight (kDa)

4.71

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 65 - 305 3.6e-45 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1174
Acc36I ACCTGC 1 cut(s) 1174
AccI GTMKAC 1 cut(s) 570
AccII CGCG 1 cut(s) 170
AciI CCGC 2 cut(s) 170, 1145
AclI AACGTT 1 cut(s) 1099
AclWI GGATC 2 cut(s) 56, 141
AcsI RAATTY 2 cut(s) 916, 1231
AcuI CTGAAG 3 cut(s) 65, 197, 1191
AfaI GTAC 2 cut(s) 344, 432
AfeI AGCGCT 1 cut(s) 76
AfiI CCNNNNNNNGG 5 cut(s) 553, 700, 850, 851, 886
AflII CTTAAG 2 cut(s) 734, 866
AgsI TTSAA 5 cut(s) 16, 107, 509, 529, 915
AjnI CCWGG 1 cut(s) 664
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 9 cut(s) 302, 364, 368, 474, 585, 965, 1025, 1135, 1207
AluI AGCT 9 cut(s) 302, 364, 368, 474, 585, 965, 1025, 1135, 1207
Alw26I GTCTC 1 cut(s) 844
AlwI GGATC 2 cut(s) 56, 141
AlwNI CAGNNNCTG 1 cut(s) 425
Aor51HI AGCGCT 1 cut(s) 76
AoxI GGCC 3 cut(s) 896, 1092, 1224
ApeKI GCWGC 5 cut(s) 299, 425, 755, 793, 974
ApoI RAATTY 2 cut(s) 916, 1231
Asp700I GAANNNNTTC 1 cut(s) 1206
AspLEI GCGC 2 cut(s) 77, 170
AspS9I GGNCC 2 cut(s) 134, 1225
AsuHPI GGTGA 2 cut(s) 49, 680
AsuNHI GCTAGC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 694
BaeI ACNNNNGTAYC 2 cut(s) 1173, 1206
BbvI GCAGC 5 cut(s) 311, 412, 742, 780, 961
BccI CCATC 4 cut(s) 214, 610, 985, 1041
BciT130I CCWGG 1 cut(s) 666
BcoDI GTCTC 1 cut(s) 844
BfaI CTAG 5 cut(s) 365, 797, 966, 978, 1199
BfoI RGCGCY 1 cut(s) 78
BfrI CTTAAG 2 cut(s) 734, 866
BfuAI ACCTGC 1 cut(s) 1174
BisI GCNGC 5 cut(s) 300, 426, 756, 794, 975
BlsI GCNGC 5 cut(s) 301, 427, 757, 795, 976
Bme1390I CCNGG 1 cut(s) 666
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 2 cut(s) 134, 1225
BmrFI CCNGG 1 cut(s) 666
BmrI ACTGGG 1 cut(s) 1134
BmsI GCATC 8 cut(s) 12, 297, 412, 622, 719, 767, 961, 1013
BmtI GCTAGC 1 cut(s) 368
BmuI ACTGGG 1 cut(s) 1134
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 885
Bsc4I CCNNNNNNNGG 5 cut(s) 553, 700, 850, 851, 886
Bse1I ACTGG 4 cut(s) 117, 136, 522, 1140
Bse3DI GCAATG 1 cut(s) 601
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 1 cut(s) 666
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 885
BseGI GGATG 5 cut(s) 225, 621, 637, 976, 1153
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 5 cut(s) 553, 700, 850, 851, 886
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 4 cut(s) 294, 618, 920, 1065
BseNI ACTGG 4 cut(s) 117, 136, 522, 1140
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 5 cut(s) 311, 412, 742, 780, 961
BseYI CCCAGC 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 3 cut(s) 898, 1094, 1226
BshVI ATCGAT 1 cut(s) 84
BslI CCNNNNNNNGG 5 cut(s) 553, 700, 850, 851, 886
BsmAI GTCTC 1 cut(s) 844
BsnI GGCC 3 cut(s) 898, 1094, 1226
Bsp1286I GDGCHC 1 cut(s) 694
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 3 cut(s) 50, 61, 146
Bsp19I CCATGG 1 cut(s) 885
BspACI CCGC 2 cut(s) 170, 1145
BspANI GGCC 3 cut(s) 898, 1094, 1226
BspCNI CTCAG 4 cut(s) 295, 619, 919, 1066
BspDI ATCGAT 1 cut(s) 84
BspFNI CGCG 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 1174
BspOI GCTAGC 1 cut(s) 368
BspPI GGATC 2 cut(s) 56, 141
BspQI GCTCTTC 1 cut(s) 1197
BspTI CTTAAG 2 cut(s) 734, 866
BsrDI GCAATG 1 cut(s) 601
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 4 cut(s) 117, 136, 522, 1140
BssECI CCNNGG 1 cut(s) 885
BssMI GATC 3 cut(s) 50, 61, 146
BssT1I CCWWGG 1 cut(s) 885
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 3 cut(s) 197, 496, 1189
Bst6I CTCTTC 3 cut(s) 41, 1116, 1197
BstAFI CTTAAG 2 cut(s) 734, 866
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 4 cut(s) 366, 592, 954, 963
BstDEI CTNAG 4 cut(s) 303, 627, 906, 1074
BstDSI CCRYGG 1 cut(s) 885
BstF5I GGATG 5 cut(s) 225, 621, 637, 976, 1153
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 77, 170
BstKTI GATC 3 cut(s) 53, 64, 149
BstMAI GTCTC 1 cut(s) 844
BstMBI GATC 3 cut(s) 50, 61, 146
BstMWI GCNNNNNNNGC 6 cut(s) 591, 683, 958, 962, 971, 983
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 1 cut(s) 690
BstSCI CCNGG 1 cut(s) 664
BstSLI GKGCMC 1 cut(s) 694
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 5 cut(s) 311, 412, 742, 780, 961
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 3 cut(s) 898, 1094, 1226
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 885
BtsCI GGATG 5 cut(s) 225, 621, 637, 976, 1153
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 5 cut(s) 479, 501, 568, 914, 1147
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 4 cut(s) 366, 592, 954, 963
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 2 cut(s) 77, 170
Cfr13I GGNCC 2 cut(s) 134, 1225
ClaI ATCGAT 1 cut(s) 84
CseI GACGC 1 cut(s) 1010
CsiI ACCWGGT 1 cut(s) 664
Csp6I GTAC 2 cut(s) 343, 431
CviAII CATG 4 cut(s) 373, 612, 687, 886
CviQI GTAC 2 cut(s) 343, 431
DdeI CTNAG 4 cut(s) 303, 627, 906, 1074
DpnI GATC 3 cut(s) 52, 63, 148
DpnII GATC 3 cut(s) 50, 61, 146
Eam1104I CTCTTC 3 cut(s) 41, 1116, 1197
EarI CTCTTC 3 cut(s) 41, 1116, 1197
Eco130I CCWWGG 1 cut(s) 885
Eco47I GGWCC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 76
Eco57I CTGAAG 3 cut(s) 65, 197, 1191
EcoO109I RGGNCCY 1 cut(s) 1225
EcoRII CCWGG 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 885
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 4 cut(s) 376, 615, 690, 889
FalI AAGNNNNNCTT 4 cut(s) 574, 606, 860, 892
FatI CATG 4 cut(s) 372, 611, 686, 885
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 300, 426, 756, 794, 975
FokI GGATG 5 cut(s) 232, 628, 644, 983, 1160
Fsp4HI GCNGC 5 cut(s) 300, 426, 756, 794, 975
FspBI CTAG 5 cut(s) 365, 797, 966, 978, 1199
GlaI GCGC 2 cut(s) 76, 169
GluI GCNGC 5 cut(s) 300, 426, 756, 794, 975
GsaI CCCAGC 1 cut(s) 792
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 3 cut(s) 898, 1094, 1226
HgaI GACGC 1 cut(s) 1010
HhaI GCGC 2 cut(s) 77, 170
Hin1II CATG 4 cut(s) 376, 615, 690, 889
Hin6I GCGC 2 cut(s) 75, 168
HinP1I GCGC 2 cut(s) 75, 168
HinfI GANTC 3 cut(s) 38, 604, 995
HphI GGTGA 2 cut(s) 49, 680
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 9 cut(s) 216, 445, 609, 628, 804, 939, 1000, 1105, 1212
Hpy188III TCNNGA 8 cut(s) 158, 395, 455, 714, 929, 1037, 1115, 1130
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 6 cut(s) 10, 75, 344, 551, 737, 1112
HpyCH4III ACNGT 3 cut(s) 197, 496, 1189
HpyCH4IV ACGT 3 cut(s) 518, 934, 1099
HpyCH4V TGCA 6 cut(s) 125, 499, 594, 686, 758, 956
HpyF10VI GCNNNNNNNGC 6 cut(s) 591, 683, 958, 962, 971, 983
HpyF3I CTNAG 4 cut(s) 303, 627, 906, 1074
HpySE526I ACGT 3 cut(s) 518, 934, 1099
Hsp92II CATG 4 cut(s) 376, 615, 690, 889
HspAI GCGC 2 cut(s) 75, 168
Kzo9I GATC 3 cut(s) 50, 61, 146
LguI GCTCTTC 1 cut(s) 1197
LmnI GCTCC 3 cut(s) 82, 1030, 1132
Lsp1109I GCAGC 5 cut(s) 311, 412, 742, 780, 961
LweI GCATC 8 cut(s) 12, 297, 412, 622, 719, 767, 961, 1013
MabI ACCWGGT 1 cut(s) 664
MaeI CTAG 5 cut(s) 365, 797, 966, 978, 1199
MaeII ACGT 3 cut(s) 518, 934, 1099
MalI GATC 3 cut(s) 52, 63, 148
MboI GATC 3 cut(s) 50, 61, 146
MboII GAAGA 4 cut(s) 58, 868, 1103, 1214
MhlI GDGCHC 1 cut(s) 694
MluCI AATT 4 cut(s) 11, 859, 916, 1231
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 9 cut(s) 44, 166, 210, 613, 622, 888, 915, 1084, 1216
MroXI GAANNNNTTC 1 cut(s) 1206
MseI TTAA 5 cut(s) 27, 581, 735, 867, 1237
MslI CAYNNNNRTG 2 cut(s) 494, 890
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 2 cut(s) 734, 866
MspR9I CCNGG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 666
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 6 cut(s) 591, 683, 958, 962, 971, 983
NcoI CCATGG 1 cut(s) 885
NdeII GATC 3 cut(s) 50, 61, 146
NheI GCTAGC 1 cut(s) 364
NlaIII CATG 4 cut(s) 376, 615, 690, 889
NspI RCATGY 1 cut(s) 690
OliI CACNNNNGTG 1 cut(s) 494
PaqCI CACCTGC 1 cut(s) 1174
PciSI GCTCTTC 1 cut(s) 1197
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 3 cut(s) 38, 604, 995
PkrI GCNGC 5 cut(s) 301, 427, 757, 795, 976
Psp1406I AACGTT 1 cut(s) 1099
Psp6I CCWGG 1 cut(s) 664
PspFI CCCAGC 1 cut(s) 788
PspGI CCWGG 1 cut(s) 664
PspPI GGNCC 2 cut(s) 134, 1225
PstNI CAGNNNCTG 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 344, 432
RsaNI GTAC 2 cut(s) 343, 431
RseI CAYNNNNRTG 2 cut(s) 494, 890
SapI GCTCTTC 1 cut(s) 1197
SaqAI TTAA 5 cut(s) 27, 581, 735, 867, 1237
SatI GCNGC 5 cut(s) 300, 426, 756, 794, 975
Sau3AI GATC 3 cut(s) 50, 61, 146
Sau96I GGNCC 2 cut(s) 134, 1225
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 1 cut(s) 694
SexAI ACCWGGT 1 cut(s) 664
SfaNI GCATC 8 cut(s) 12, 297, 412, 622, 719, 767, 961, 1013
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 494, 890
SmlI CTYRAG 4 cut(s) 467, 734, 866, 1220
SmoI CTYRAG 4 cut(s) 467, 734, 866, 1220
Sse9I AATT 4 cut(s) 11, 859, 916, 1231
SsiI CCGC 2 cut(s) 170, 1145
SspMI CTAG 5 cut(s) 365, 797, 966, 978, 1199
StyD4I CCNGG 1 cut(s) 664
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 3 cut(s) 197, 496, 1189
TaiI ACGT 3 cut(s) 521, 937, 1102
TaqI TCGA 3 cut(s) 36, 84, 1065
TasI AATT 4 cut(s) 11, 859, 916, 1231
TatI WGTACW 1 cut(s) 430
TfiI GAWTC 3 cut(s) 38, 604, 995
Tru1I TTAA 5 cut(s) 27, 581, 735, 867, 1237
Tru9I TTAA 5 cut(s) 27, 581, 735, 867, 1237
TscAI CASTG 5 cut(s) 486, 501, 568, 914, 1147
TseI GCWGC 5 cut(s) 299, 425, 755, 793, 974
TspDTI ATGAA 5 cut(s) 255, 389, 612, 738, 1059
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 5 cut(s) 486, 501, 568, 914, 1147
Vha464I CTTAAG 2 cut(s) 734, 866
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 2 cut(s) 916, 1231
XceI RCATGY 1 cut(s) 690
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 1206
XspI CTAG 5 cut(s) 365, 797, 966, 978, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.